THBS2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	THBS2
Entrez Gene ID	414837
Full Name	thrombospondin 2
Synonyms	thrombospondin-2
General protein information	Preferred Names thrombospondin 2 Names thrombospondin-2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015284053.1	XP_015139539.1	thrombospondin-2 isoform X2
NM_001001755.1	NP_001001755.1	thrombospondin-2 precursor
XM_015284051.2	XP_015139537.1	thrombospondin-2 isoform X1
XM_015284051.1	XP_015139537.1	thrombospondin-2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	THBS2	NP_003238.2
Macaca mulatta (Rhesus monkey)	THBS2	XP_001096616.2
Mus musculus (house mouse)	Thbs2	NP_035711.2
Gallus gallus (chicken)	THBS2	NP_001001755.1
Danio rerio (zebrafish)	thbs2b	XP_694690.6
Danio rerio (zebrafish)	LOC100535241	XP_003199793.2
Pan troglodytes (chimpanzee)	THBS2	XP_003311640.2
Canis lupus familiaris (dog)	THBS2	NP_001184085.1
Danio rerio (zebrafish)	thbs2a	XP_005157071.1
Bos taurus (cattle)	THBS2	NP_789861.1
Rattus norvegicus (Norway rat)	Thbs2	NP_001162609.1
Xenopus tropicalis (tropical clawed frog)	thbs2	XP_002931559.2

Gene Ontology
Bibliography

Related articles in PubMed

Cloning and sequencing of chicken thrombospondin.
Lawler J, Duquette M, Ferro P
The Journal of biological chemistry266(13)8039-43(1991 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following THBS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa27135	NM_001001755.1 Latest version!	Gallus gallus thrombospondin 2 (THBS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa33580	XM_015284051.2 Latest version!	Gallus gallus thrombospondin 2 (THBS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa33581	XM_015284053.1 Latest version!	Gallus gallus thrombospondin 2 (THBS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OGa33580	XM_015284051.1	Gallus gallus thrombospondin 2 (THBS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THBS2 ( NM_001001755.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001001755.1 Gallus gallus thrombospondin 2 (THBS2), mRNA.

Price & Availability↑

CloneID	OGa27135
Clone ID Related Accession (Same CDS sequence)	NM_001001755.1
Accession Version	NM_001001755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550160000000
Organism	Gallus gallus(chicken)
Product	thrombospondin-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M60853.1. On Jan 18, 2005 this sequence version replaced XM_419599.1. ##Evidence-Data-START## Transcript exon combination :: M60853.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTGCAGA GGAGCAGGCT GTTGTGGCTT GCTGTCTTCA TAACCCTGTG GGTTTCCTCA 
GATGCTCAGG ATGATGCCAA AGAAGAGGAG AATACCTTTG ATTTACTTCA AATCAGTAAC 
ATAAACCGAA AGACAATTGG AGCAAAATTG TTTCGGGGTC CAGATCCCGC TATCCCAGCA 
TATCGTTTCA TTCGGTTTGA CCATATACCT CCATTTAAAC CAGAGAAGTT AAAAAAGATT 
GTTAAACTCA TACGGCAAAA TGAGGGCTTT ATCCTTTCAG CCACCCTGAG ACAGGACAGG 
CAATCTAGAG GCACTATCCT TGCTTTAGAG GGTCCTGGCA TCAGTGAGAG GCAGTTTGAG 
ATAATTTCCA ATGGCCGAGC CAACACCCTG GACCTCATTT ATTGGGTGGA TGGCTTCCAG 
AACGTGATTT CCCTGGAAGA CGTGGACCTT GCTGACTCGC AGTGGAAGAA CCTCACCGTG 
CAAGTAACAG GAGAGAACTA CAACCTCTAT GTTGGCTGTG ACCTTATTGA CAGCTTCATC 
CTGGAGGAGC CTTTCTATGA GCAACTGAAA GCAGAGAACA GCAGAATGTA TGTGGCGAAA 
GGGTCCATTC GGGAGAATCA TTTCAGGGGT CTTTTGCAAA ATATTCATTT AATCTTTGAT 
ACTTCAATAG AGGATGTTCT ACGTAAAAAA GGATGCCAGA GGAGCCAATC AACTGAAGTG 
AACACCATCA ATGAGAGCAC TGAGATTCTT CACTTGAGCC CTGCTGTCAC GACTGAGTAT 
GTAGGTGAAA AGACAGAGAA GAAGGCTGAG TTCTGTGACC GCTCTTGTGA AGAGCTGGGA 
ACAATGTTCA CTGAACTCAC TGGCCTGCGC ATTGTGGTGA ACAATTTAGC TGATAACCTC 
CAAAAAGTGT CTGAAGAGAA CCAAATAATG TGGGAATTGA TTGGCCCTAA CAAAACACTC 
AAGAACCAAT CAGTTTGCTG GCAGGATGGC CGAGTCTTTG CAGATAGTGA AAGCTGGATT 
GTAGATAGCT GCACAAAGTG CACCTGCCAG GACTCTAAAA TTGTGTGCCA TCAAATCACC 
TGTCCACCAG TTTCTTGTGC TGATCCGTCC TTTATTGAAG GTGAATGCTG TCCAGTCTGC 
TCTCACTCTG ATGATAGTGA GGAAGGCTGG TCACCCTGGT CAGACTGGAC AAAGTGCTCA 
GTTACCTGTG GCTCTGGGAC TCAGATGAGA GGAAGGTCTT GTGATGTCAC CAGGAGTGCT 
TGTACAGGCC CACACATTCA GACGAGGATG TGCAGCTTCA AGAAATGTGA CCATCGCATA 
CGTCAAGATG GTGGTTGGAG TCACTGGTCT CCCTGGTCCT CATGTTCAGT TACCTGTGGA 
GTAGGAAATA TCACCCGCAT CCGCCTCTGC AATTCCCCTA TTCCCCAGAT GGGTGGGAAA 
AACTGTGTGG GAAATGGACG TGAAACAGAG AAATGTGAGA AGGCCCCGTG CCCAGTGAAT 
GGCCAGTGGG GTCCTTGGTC TCCATGGTCT GCTTGCACTG TTACCTGTGG AGGAGGAATC 
CGTGAGAGGT CTCGCCTCTG TAACAGTCCA GAGCCACAGT ATGGAGGAAA GCCATGTGTG 
GGAGATACCA AGCAACATGA TATGTGCAAT AAAAGGGATT GCCCAATTGA TGGTTGTTTG 
TCAAATCCTT GCTTTCCTGG AGCAGAATGC AACAGTTACC CGGATGGATC GTGGTCATGT 
GGGCCATGCC CAGCAGGTTT CCTTGGCAAT GGTACAGTCT GTGAGGATCT TGATGAGTGT 
ATAGCTGTGT CAGATGTTTG CTTTAAGGTG AATCAGGTCC ATCGCTGTGT GAACACAAAT 
CCGGGTTTCC ATTGCTTGCC ATGTCCTCCG CGTTATAAAG GAAGTCAGCC TTACGGAGTA 
GGGCTGGAAG TTGCCAAGAC AGAGAAGCAA GTCTGTGAAC CTGAGAATCC ATGCAAAGAC 
AAGACACACA GCTGCCATAA ATCTGCAGAG TGCATTTATC TGGGTCATTT CAGTGATCCT 
ATGTACAAAT GTGAATGTAG GACAGGTTAT GCTGGTGATG GACGCATCTG TGGTGAGGAT 
TCAGATTTGG ATGGATGGCC AAATAATAAC TTGGTTTGTG CTGCTAATGC TACGTATCAC 
TGTGTGAAGG ATAACTGCCC CCTCCTGCCT AACTCTGGCC AAGAAGACTT TGATAAAGAT 
GGCAAAGGAG ATGCCTGTGA TGAGGATGAT GACAATGATG GTGTTGAAGA TGACAAAGAC 
AATTGCCCTC TTCTGTTCAA TCCTCGTCAG TTTGATTATG ACAAAGATGA AGTTGGTGAC 
CGCTGTGATA ATTGCCCTTA TGTGCACAAC CCCGCACAGA TTGACACAGA TAATAATGGG 
GAGGGTGATT CATGTGCTGT GGATATTGAT GGAGATGACA TTTTTAATGA ACGAGATAAT 
TGTCCTTACG TTTATAATAC AGATCAGAGT GATACAGATG GTGATGGAGT TGGTGATCAG 
TGTGATAACT GTCCGCTAAT GCATAATCCA GACCAGACTG ATGCAGATAA CGATCTTGTT 
GGTGACCAGT GTGACAACAA TGAGGACATA GATGAAGATG GCCACCAGAA CAACCAAGAT 
AACTGCCCTT ACATCCCCAA TGCTAACCAG GCTGACCATG ATAAAGATGG TAAAGGGGAT 
GCCTGTGATC CTGATGATGA CAATGATGGT ATTCCAGATG ACAGGGACAA TTGTCGCCTC 
AGATACAACC CTGAGCAGGA GGACTCTGAT GGTGATGGCA GAGGTGATAT TTGCAAAGAT 
GACTTTGACG ATGACAATGT TCCAGATATT TTTGATGTAT GTCCTGAAAA CAATGCCATC 
AGTGAAACTG ATTTCAGAAA GTTCCAGATG GTCCCTCTGG ACCCCAAGGG AACAGCTCAG 
ATTGATCCCA ACTGGGTTAT TCGCCACCAA GGCAAGGAAC TGGTGCAGAC TGCCAACTCT 
GATCCTGGAA TAGCTGTAGG TTATGATGAG TTCAGCTCTG TTGACTTCAG TGGCACATTC 
TATGTTAACA CAGACCGTGA TGATGACTAT GCTGGCTTTG TTTTTGGCTA CCAGTCCAGC 
AGCCGTTTTT ATGTTCTGAT GTGGAAGCAG GTCACTCAGA CCTACTGGGA GGACAAACCC 
ACCAGGGCTT ATGGCTATTC TGGTGTGTCA CTCAAGGTAG TGAATTCAAC AACTGGTACT 
GGGGAACACT TGCGAAATGC TCTTTGGCAT ACAGGAAACA CCCCTGGACA GGTGCGCACT 
TTATGGCATG ATCCTAAGAA CATTGGCTGG AAAGATTATA CTGCATACAG GTGGCATTTG 
ATTCACAGGC CTAAAACAGG ACTAATAAAA GTTCTAGTGT ATGAAGGGAA GCAAGTCATG 
GTGGATTCTG GACCAATCTA TGATACAACC TTTGCTGGTG GACGATTAGG TCTATTTGTC 
TTCTCTCAAG AAATGGTCTA TTTCTCTGAC CTCAAATATG AATGCAGAGA TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001755.1
CDS	1..3537
Translation	MLQRSRLLWLAVFITLWVSSDAQDDAKEEENTFDLLQISNINRKTIGAKLFRGPDPAIPAYRFIRFDHIPPFKPEKLKKIVKLIRQNEGFILSATLRQDRQSRGTILALEGPGISERQFEIISNGRANTLDLIYWVDGFQNVISLEDVDLADSQWKNLTVQVTGENYNLYVGCDLIDSFILEEPFYEQLKAENSRMYVAKGSIRENHFRGLLQNIHLIFDTSIEDVLRKKGCQRSQSTEVNTINESTEILHLSPAVTTEYVGEKTEKKAEFCDRSCEELGTMFTELTGLRIVVNNLADNLQKVSEENQIMWELIGPNKTLKNQSVCWQDGRVFADSESWIVDSCTKCTCQDSKIVCHQITCPPVSCADPSFIEGECCPVCSHSDDSEEGWSPWSDWTKCSVTCGSGTQMRGRSCDVTRSACTGPHIQTRMCSFKKCDHRIRQDGGWSHWSPWSSCSVTCGVGNITRIRLCNSPIPQMGGKNCVGNGRETEKCEKAPCPVNGQWGPWSPWSACTVTCGGGIRERSRLCNSPEPQYGGKPCVGDTKQHDMCNKRDCPIDGCLSNPCFPGAECNSYPDGSWSCGPCPAGFLGNGTVCEDLDECIAVSDVCFKVNQVHRCVNTNPGFHCLPCPPRYKGSQPYGVGLEVAKTEKQVCEPENPCKDKTHSCHKSAECIYLGHFSDPMYKCECRTGYAGDGRICGEDSDLDGWPNNNLVCAANATYHCVKDNCPLLPNSGQEDFDKDGKGDACDEDDDNDGVEDDKDNCPLLFNPRQFDYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDSCAVDIDGDDIFNERDNCPYVYNTDQSDTDGDGVGDQCDNCPLMHNPDQTDADNDLVGDQCDNNEDIDEDGHQNNQDNCPYIPNANQADHDKDGKGDACDPDDDNDGIPDDRDNCRLRYNPEQEDSDGDGRGDICKDDFDDDNVPDIFDVCPENNAISETDFRKFQMVPLDPKGTAQIDPNWVIRHQGKELVQTANSDPGIAVGYDEFSSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVLMWKQVTQTYWEDKPTRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTPGQVRTLWHDPKNIGWKDYTAYRWHLIHRPKTGLIKVLVYEGKQVMVDSGPIYDTTFAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	NM_001001755.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thrombospondin 2 (THBS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001755.1

ORF Insert Sequence:

ATGCTGCAGA GGAGCAGGCT GTTGTGGCTT GCTGTCTTCA TAACCCTGTG GGTTTCCTCA 
GATGCTCAGG ATGATGCCAA AGAAGAGGAG AATACCTTTG ATTTACTTCA AATCAGTAAC 
ATAAACCGAA AGACAATTGG AGCAAAATTG TTTCGGGGTC CAGATCCCGC TATCCCAGCA 
TATCGTTTCA TTCGGTTTGA CCATATACCT CCATTTAAAC CAGAGAAGTT AAAAAAGATT 
GTTAAACTCA TACGGCAAAA TGAGGGCTTT ATCCTTTCAG CCACCCTGAG ACAGGACAGG 
CAATCTAGAG GCACTATCCT TGCTTTAGAG GGTCCTGGCA TCAGTGAGAG GCAGTTTGAG 
ATAATTTCCA ATGGCCGAGC CAACACCCTG GACCTCATTT ATTGGGTGGA TGGCTTCCAG 
AACGTGATTT CCCTGGAAGA CGTGGACCTT GCTGACTCGC AGTGGAAGAA CCTCACCGTG 
CAAGTAACAG GAGAGAACTA CAACCTCTAT GTTGGCTGTG ACCTTATTGA CAGCTTCATC 
CTGGAGGAGC CTTTCTATGA GCAACTGAAA GCAGAGAACA GCAGAATGTA TGTGGCGAAA 
GGGTCCATTC GGGAGAATCA TTTCAGGGGT CTTTTGCAAA ATATTCATTT AATCTTTGAT 
ACTTCAATAG AGGATGTTCT ACGTAAAAAA GGATGCCAGA GGAGCCAATC AACTGAAGTG 
AACACCATCA ATGAGAGCAC TGAGATTCTT CACTTGAGCC CTGCTGTCAC GACTGAGTAT 
GTAGGTGAAA AGACAGAGAA GAAGGCTGAG TTCTGTGACC GCTCTTGTGA AGAGCTGGGA 
ACAATGTTCA CTGAACTCAC TGGCCTGCGC ATTGTGGTGA ACAATTTAGC TGATAACCTC 
CAAAAAGTGT CTGAAGAGAA CCAAATAATG TGGGAATTGA TTGGCCCTAA CAAAACACTC 
AAGAACCAAT CAGTTTGCTG GCAGGATGGC CGAGTCTTTG CAGATAGTGA AAGCTGGATT 
GTAGATAGCT GCACAAAGTG CACCTGCCAG GACTCTAAAA TTGTGTGCCA TCAAATCACC 
TGTCCACCAG TTTCTTGTGC TGATCCGTCC TTTATTGAAG GTGAATGCTG TCCAGTCTGC 
TCTCACTCTG ATGATAGTGA GGAAGGCTGG TCACCCTGGT CAGACTGGAC AAAGTGCTCA 
GTTACCTGTG GCTCTGGGAC TCAGATGAGA GGAAGGTCTT GTGATGTCAC CAGGAGTGCT 
TGTACAGGCC CACACATTCA GACGAGGATG TGCAGCTTCA AGAAATGTGA CCATCGCATA 
CGTCAAGATG GTGGTTGGAG TCACTGGTCT CCCTGGTCCT CATGTTCAGT TACCTGTGGA 
GTAGGAAATA TCACCCGCAT CCGCCTCTGC AATTCCCCTA TTCCCCAGAT GGGTGGGAAA 
AACTGTGTGG GAAATGGACG TGAAACAGAG AAATGTGAGA AGGCCCCGTG CCCAGTGAAT 
GGCCAGTGGG GTCCTTGGTC TCCATGGTCT GCTTGCACTG TTACCTGTGG AGGAGGAATC 
CGTGAGAGGT CTCGCCTCTG TAACAGTCCA GAGCCACAGT ATGGAGGAAA GCCATGTGTG 
GGAGATACCA AGCAACATGA TATGTGCAAT AAAAGGGATT GCCCAATTGA TGGTTGTTTG 
TCAAATCCTT GCTTTCCTGG AGCAGAATGC AACAGTTACC CGGATGGATC GTGGTCATGT 
GGGCCATGCC CAGCAGGTTT CCTTGGCAAT GGTACAGTCT GTGAGGATCT TGATGAGTGT 
ATAGCTGTGT CAGATGTTTG CTTTAAGGTG AATCAGGTCC ATCGCTGTGT GAACACAAAT 
CCGGGTTTCC ATTGCTTGCC ATGTCCTCCG CGTTATAAAG GAAGTCAGCC TTACGGAGTA 
GGGCTGGAAG TTGCCAAGAC AGAGAAGCAA GTCTGTGAAC CTGAGAATCC ATGCAAAGAC 
AAGACACACA GCTGCCATAA ATCTGCAGAG TGCATTTATC TGGGTCATTT CAGTGATCCT 
ATGTACAAAT GTGAATGTAG GACAGGTTAT GCTGGTGATG GACGCATCTG TGGTGAGGAT 
TCAGATTTGG ATGGATGGCC AAATAATAAC TTGGTTTGTG CTGCTAATGC TACGTATCAC 
TGTGTGAAGG ATAACTGCCC CCTCCTGCCT AACTCTGGCC AAGAAGACTT TGATAAAGAT 
GGCAAAGGAG ATGCCTGTGA TGAGGATGAT GACAATGATG GTGTTGAAGA TGACAAAGAC 
AATTGCCCTC TTCTGTTCAA TCCTCGTCAG TTTGATTATG ACAAAGATGA AGTTGGTGAC 
CGCTGTGATA ATTGCCCTTA TGTGCACAAC CCCGCACAGA TTGACACAGA TAATAATGGG 
GAGGGTGATT CATGTGCTGT GGATATTGAT GGAGATGACA TTTTTAATGA ACGAGATAAT 
TGTCCTTACG TTTATAATAC AGATCAGAGT GATACAGATG GTGATGGAGT TGGTGATCAG 
TGTGATAACT GTCCGCTAAT GCATAATCCA GACCAGACTG ATGCAGATAA CGATCTTGTT 
GGTGACCAGT GTGACAACAA TGAGGACATA GATGAAGATG GCCACCAGAA CAACCAAGAT 
AACTGCCCTT ACATCCCCAA TGCTAACCAG GCTGACCATG ATAAAGATGG TAAAGGGGAT 
GCCTGTGATC CTGATGATGA CAATGATGGT ATTCCAGATG ACAGGGACAA TTGTCGCCTC 
AGATACAACC CTGAGCAGGA GGACTCTGAT GGTGATGGCA GAGGTGATAT TTGCAAAGAT 
GACTTTGACG ATGACAATGT TCCAGATATT TTTGATGTAT GTCCTGAAAA CAATGCCATC 
AGTGAAACTG ATTTCAGAAA GTTCCAGATG GTCCCTCTGG ACCCCAAGGG AACAGCTCAG 
ATTGATCCCA ACTGGGTTAT TCGCCACCAA GGCAAGGAAC TGGTGCAGAC TGCCAACTCT 
GATCCTGGAA TAGCTGTAGG TTATGATGAG TTCAGCTCTG TTGACTTCAG TGGCACATTC 
TATGTTAACA CAGACCGTGA TGATGACTAT GCTGGCTTTG TTTTTGGCTA CCAGTCCAGC 
AGCCGTTTTT ATGTTCTGAT GTGGAAGCAG GTCACTCAGA CCTACTGGGA GGACAAACCC 
ACCAGGGCTT ATGGCTATTC TGGTGTGTCA CTCAAGGTAG TGAATTCAAC AACTGGTACT 
GGGGAACACT TGCGAAATGC TCTTTGGCAT ACAGGAAACA CCCCTGGACA GGTGCGCACT 
TTATGGCATG ATCCTAAGAA CATTGGCTGG AAAGATTATA CTGCATACAG GTGGCATTTG 
ATTCACAGGC CTAAAACAGG ACTAATAAAA GTTCTAGTGT ATGAAGGGAA GCAAGTCATG 
GTGGATTCTG GACCAATCTA TGATACAACC TTTGCTGGTG GACGATTAGG TCTATTTGTC 
TTCTCTCAAG AAATGGTCTA TTTCTCTGAC CTCAAATATG AATGCAGAGA TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS2 ( XM_015284051.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139537.1 Gallus gallus thrombospondin 2 (THBS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa33580
Clone ID Related Accession (Same CDS sequence)	XM_015284051.2 , XM_015284051.1
Accession Version	XM_015284051.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	thrombospondin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284051.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGACG GCGGTAGGGC GCCGCGATCC GCTCCGCAAC GCCGGCAGCG CCCCGGGAGC 
AGGCAGAGCC CTCTCGTCCC GTCGGCTCTC CTTATGCCAG GAGTTATGCT GCAGAGGAGC 
AGGCTGTTGT GGCTTGCTGT CTTCATAACC CTGTGGGTTT CCTCAGATGC TCAGGATGAT 
GCCAAGGAAG AGGAGAATAC CTTTGATTTA CTTCAAATCA GTAACATAAA CCGAAAGACA 
ATTGGAGCAA AATTGTTTCG GGGTCCAGAT CCCGCTATCC CAGCATATCG TTTCATTCGG 
TTTGACCATA TACCTCCATT TAAACCAGAG AAGTTAAAAA AGATTGTTAA ACTCATACGG 
CAAAATGAGG GCTTTATCCT TTCAGCCACC CTGAGACAGG ACAGGCAATC TAGAGGCACT 
ATCCTTGCTT TAGAGGGTCC TGGCATCAGT GAGAGGCAGT TTGAGATAAT TTCCAATGGC 
CGAGCCAACA CCCTGGACCT CATTTATTGG GTGGATGGCT TCCAGAACGT GATTTCCCTG 
GAAGACGTGG ACCTTGCTGA CTCGCAGTGG AAGAATCTCA CCGTGCAAGT AACAGGAGAG 
AACTACAACC TCTATGTTGG CTGTGACCTT ATTGACAGCT TCATCCTGGA GGAGCCTTTC 
TATGAGCAAC TGAAAGCAGA GAACAGCAGA ATGTATGTGG CGAAAGGGTC CATTCGGGAG 
AATCATTTCA GGGGTCTTTT GCAAAATATT CATTTAATCT TTGATACTTC AATAGAGGAT 
GTTCTACGTA AAAAAGGATG CCAGAGGAGC CAATCAACTG AAGTGAACAC CATCAATGAG 
AGCACTGAGA TCCTTCACTT GAGCCCTGCT GTCACAACTG AGTATGTAGG TGAAAAGACA 
GAGAAGAAGG CTGAGTTCTG TGACCGCTCT TGTGAAGAGC TGGGAACAAT GTTCACTGAA 
CTCACTGGCC TGCGCATTGT GGTGAACAAT TTAGCTGATA ACCTCCAGAA AGTGTCTGAA 
GAGAATCAAA TAATGTGGGA ATTGATTGGC CCTAACAAAA CACTCAAGAA CCAATCAGTT 
TGCTGGCAGG ATGGCCGAGT CTTTGCAGAT AGTGAAAGCT GGATTGTAGA TAGCTGCACA 
AAGTGCACCT GCCAGGACTC TAAAATTGTG TGCCATCAAA TCACCTGTCC ACCAGTTTCT 
TGTGCTGATC CGTCCTTTAT TGAAGGTGAA TGCTGTCCAG TCTGCTCTCA CTCTGATGAT 
AGTGAGGAAG GCTGGTCACC CTGGTCAGAC TGGACAAAGT GCTCAGTTAC CTGTGGCTCT 
GGGACACAGA TGAGAGGAAG GTCTTGTGAT GTCACCAGGA GTGCTTGTAC AGGCCCACAC 
ATTCAGACGA GAATGTGCAG CTTCAAGAAA TGTGACCATC GCATACGTCA AGATGGTGGT 
TGGAGTCACT GGTCTCCCTG GTCCTCATGT TCAGTTACCT GTGGAGTAGG AAACATCACC 
CGCATCCGCC TCTGCAATTC CCCTATTCCC CAGATGGGTG GGAAAAACTG TGTGGGAAAT 
GGACGTGAAA CAGAGAAATG TGAGAAGGCC CCGTGCCCAG TGAATGGCCA GTGGGGTCCT 
TGGTCTCCAT GGTCTGCTTG CACTGTTACC TGTGGAGGAG GAATCCGTGA GAGGTCTCGC 
CTCTGTAACA GTCCAGAGCC ACAGTATGGA GGAAAGCCGT GTGTGGGAGA TACCAAGCAA 
CATGATATGT GCAATAAAAG GGATTGCCCA ATTGATGGTT GTTTGTCAAA TCCTTGCTTT 
CCTGGAGCAG AATGCAACAG TTACCCGGAT GGATCGTGGT CATGTGGGCC ATGCCCAGCA 
GGTTTCCTTG GCAATGGTAC AGTCTGTGAG GATCTTGATG AGTGTATAGC TGTGTCAGAT 
GTTTGCTTTA AGGTGAATCA GGTCCATCGC TGTGTGAACA CAAATCCGGG TTTCCATTGC 
TTGCCATGTC CTCCGCGTTA TAAAGGAAGT CAGCCTTACG GAGTAGGGCT GGAAGTTGCC 
AAGACAGAGA AGCAAGTCTG TGAACCTGAG AATCCATGCA AAGACAAGAC ACACAGCTGC 
CATAAATCTG CAGAGTGCAT TTATCTGGGT CATTTCAGTG ATCCTATGTA CAAATGTGAA 
TGTAGGACAG GTTATGCTGG TGATGGACGC ATCTGTGGTG AGGATTCAGA TTTGGATGGA 
TGGCCAAATA ATAACTTGGT TTGTGCTGCT AATGCTACGT ATCACTGTGT GAAGGATAAC 
TGCCCCCTCC TGCCTAACTC TGGCCAAGAA GACTTTGATA AAGATGGCAA AGGAGATGCC 
TGTGATGAGG ATGATGACAA TGATGGTGTT GAAGATGACA AAGACAATTG CCCTCTTCTG 
TTCAATCCTC GTCAGTTTGA TTATGACAAA GATGAAGTTG GTGACCGCTG TGATAATTGC 
CCTTATGTGC ACAACCCCGC ACAGATTGAC ACAGATAATA ATGGGGAGGG TGATTCATGT 
GCTGTGGATA TTGATGGAGA TGACATTTTT AATGAACGAG ATAATTGTCC TTACGTTTAT 
AATACAGATC AGAGTGATAC AGATGGTGAT GGAGTTGGTG ATCAGTGTGA TAACTGTCCG 
CTAATGCATA ATCCAGACCA GACTGATGCA GATAACGATC TTGTTGGTGA CCAATGTGAC 
AACAATGAGG ACATAGATGA AGATGGCCAC CAGAACAACC AAGATAACTG CCCTTACATC 
CCCAATGCTA ACCAGGCTGA CCATGATAAA GATGGTAAAG GGGATGCCTG TGATCCTGAT 
GATGACAATG ATGGTATTCC AGATGACAGG GACAATTGTC GCCTCAGATA CAACCCTGAG 
CAGGAGGACT CTGATGGTGA TGGCAGAGGT GATATTTGCA AAGATGACTT TGACGATGAC 
AATGTTCCAG ATATTTTTGA TGTATGTCCT GAAAACAATG CCATCAGTGA AACTGATTTC 
AGAAAGTTCC AGATGGTCCC TCTGGACCCC AAGGGAACAG CTCAGATTGA TCCCAACTGG 
GTTATTCGCC ACCAAGGCAA GGAACTGGTG CAGACTGCCA ACTCTGATCC TGGAATAGCT 
GTAGGTTATG ATGAGTTCAG CTCTGTTGAC TTCAGTGGCA CATTCTATGT TAACACAGAC 
CGTGATGATG ACTATGCTGG CTTTGTTTTT GGCTACCAGT CCAGCAGCCG TTTTTATGTT 
CTAATGTGGA AGCAGGTCAC TCAAACCTAC TGGGAGGACA AACCCACGAG GGCTTATGGC 
TATTCTGGTG TGTCACTCAA GGTAGTGAAT TCAACAACTG GTACTGGGGA ACACTTGCGA 
AATGCTCTTT GGCATACAGG AAACACCCCC GGACAGGTGC GCACTTTATG GCATGATCCT 
AAGAACATTG GCTGGAAAGA TTATACTGCA TACAGGTGGC ATTTGATTCA CAGGCCTAAA 
ACAGGACTAA TAAAAGTTCT AGTGTATGAA GGGAAGCAAG TCATGGTGGA TTCTGGACCA 
ATCTATGATA CAACCTTTGC TGGTGGACGA TTAGGTCTAT TTGTCTTCTC TCAAGAAATG 
GTCTATTTCT CTGACCTCAA ATATGAATGC AGAGATGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139537.1
CDS	100..3741
Translation	MCDGGRAPRSAPQRRQRPGSRQSPLVPSALLMPGVMLQRSRLLWLAVFITLWVSSDAQDDAKEEENTFDLLQISNINRKTIGAKLFRGPDPAIPAYRFIRFDHIPPFKPEKLKKIVKLIRQNEGFILSATLRQDRQSRGTILALEGPGISERQFEIISNGRANTLDLIYWVDGFQNVISLEDVDLADSQWKNLTVQVTGENYNLYVGCDLIDSFILEEPFYEQLKAENSRMYVAKGSIRENHFRGLLQNIHLIFDTSIEDVLRKKGCQRSQSTEVNTINESTEILHLSPAVTTEYVGEKTEKKAEFCDRSCEELGTMFTELTGLRIVVNNLADNLQKVSEENQIMWELIGPNKTLKNQSVCWQDGRVFADSESWIVDSCTKCTCQDSKIVCHQITCPPVSCADPSFIEGECCPVCSHSDDSEEGWSPWSDWTKCSVTCGSGTQMRGRSCDVTRSACTGPHIQTRMCSFKKCDHRIRQDGGWSHWSPWSSCSVTCGVGNITRIRLCNSPIPQMGGKNCVGNGRETEKCEKAPCPVNGQWGPWSPWSACTVTCGGGIRERSRLCNSPEPQYGGKPCVGDTKQHDMCNKRDCPIDGCLSNPCFPGAECNSYPDGSWSCGPCPAGFLGNGTVCEDLDECIAVSDVCFKVNQVHRCVNTNPGFHCLPCPPRYKGSQPYGVGLEVAKTEKQVCEPENPCKDKTHSCHKSAECIYLGHFSDPMYKCECRTGYAGDGRICGEDSDLDGWPNNNLVCAANATYHCVKDNCPLLPNSGQEDFDKDGKGDACDEDDDNDGVEDDKDNCPLLFNPRQFDYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDSCAVDIDGDDIFNERDNCPYVYNTDQSDTDGDGVGDQCDNCPLMHNPDQTDADNDLVGDQCDNNEDIDEDGHQNNQDNCPYIPNANQADHDKDGKGDACDPDDDNDGIPDDRDNCRLRYNPEQEDSDGDGRGDICKDDFDDDNVPDIFDVCPENNAISETDFRKFQMVPLDPKGTAQIDPNWVIRHQGKELVQTANSDPGIAVGYDEFSSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVLMWKQVTQTYWEDKPTRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTPGQVRTLWHDPKNIGWKDYTAYRWHLIHRPKTGLIKVLVYEGKQVMVDSGPIYDTTFAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	XM_015284051.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thrombospondin 2 (THBS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284051.2

ORF Insert Sequence:

ATGTGCGACG GCGGTAGGGC GCCGCGATCC GCTCCGCAAC GCCGGCAGCG CCCCGGGAGC 
AGGCAGAGCC CTCTCGTCCC GTCGGCTCTC CTTATGCCAG GAGTTATGCT GCAGAGGAGC 
AGGCTGTTGT GGCTTGCTGT CTTCATAACC CTGTGGGTTT CCTCAGATGC TCAGGATGAT 
GCCAAGGAAG AGGAGAATAC CTTTGATTTA CTTCAAATCA GTAACATAAA CCGAAAGACA 
ATTGGAGCAA AATTGTTTCG GGGTCCAGAT CCCGCTATCC CAGCATATCG TTTCATTCGG 
TTTGACCATA TACCTCCATT TAAACCAGAG AAGTTAAAAA AGATTGTTAA ACTCATACGG 
CAAAATGAGG GCTTTATCCT TTCAGCCACC CTGAGACAGG ACAGGCAATC TAGAGGCACT 
ATCCTTGCTT TAGAGGGTCC TGGCATCAGT GAGAGGCAGT TTGAGATAAT TTCCAATGGC 
CGAGCCAACA CCCTGGACCT CATTTATTGG GTGGATGGCT TCCAGAACGT GATTTCCCTG 
GAAGACGTGG ACCTTGCTGA CTCGCAGTGG AAGAATCTCA CCGTGCAAGT AACAGGAGAG 
AACTACAACC TCTATGTTGG CTGTGACCTT ATTGACAGCT TCATCCTGGA GGAGCCTTTC 
TATGAGCAAC TGAAAGCAGA GAACAGCAGA ATGTATGTGG CGAAAGGGTC CATTCGGGAG 
AATCATTTCA GGGGTCTTTT GCAAAATATT CATTTAATCT TTGATACTTC AATAGAGGAT 
GTTCTACGTA AAAAAGGATG CCAGAGGAGC CAATCAACTG AAGTGAACAC CATCAATGAG 
AGCACTGAGA TCCTTCACTT GAGCCCTGCT GTCACAACTG AGTATGTAGG TGAAAAGACA 
GAGAAGAAGG CTGAGTTCTG TGACCGCTCT TGTGAAGAGC TGGGAACAAT GTTCACTGAA 
CTCACTGGCC TGCGCATTGT GGTGAACAAT TTAGCTGATA ACCTCCAGAA AGTGTCTGAA 
GAGAATCAAA TAATGTGGGA ATTGATTGGC CCTAACAAAA CACTCAAGAA CCAATCAGTT 
TGCTGGCAGG ATGGCCGAGT CTTTGCAGAT AGTGAAAGCT GGATTGTAGA TAGCTGCACA 
AAGTGCACCT GCCAGGACTC TAAAATTGTG TGCCATCAAA TCACCTGTCC ACCAGTTTCT 
TGTGCTGATC CGTCCTTTAT TGAAGGTGAA TGCTGTCCAG TCTGCTCTCA CTCTGATGAT 
AGTGAGGAAG GCTGGTCACC CTGGTCAGAC TGGACAAAGT GCTCAGTTAC CTGTGGCTCT 
GGGACACAGA TGAGAGGAAG GTCTTGTGAT GTCACCAGGA GTGCTTGTAC AGGCCCACAC 
ATTCAGACGA GAATGTGCAG CTTCAAGAAA TGTGACCATC GCATACGTCA AGATGGTGGT 
TGGAGTCACT GGTCTCCCTG GTCCTCATGT TCAGTTACCT GTGGAGTAGG AAACATCACC 
CGCATCCGCC TCTGCAATTC CCCTATTCCC CAGATGGGTG GGAAAAACTG TGTGGGAAAT 
GGACGTGAAA CAGAGAAATG TGAGAAGGCC CCGTGCCCAG TGAATGGCCA GTGGGGTCCT 
TGGTCTCCAT GGTCTGCTTG CACTGTTACC TGTGGAGGAG GAATCCGTGA GAGGTCTCGC 
CTCTGTAACA GTCCAGAGCC ACAGTATGGA GGAAAGCCGT GTGTGGGAGA TACCAAGCAA 
CATGATATGT GCAATAAAAG GGATTGCCCA ATTGATGGTT GTTTGTCAAA TCCTTGCTTT 
CCTGGAGCAG AATGCAACAG TTACCCGGAT GGATCGTGGT CATGTGGGCC ATGCCCAGCA 
GGTTTCCTTG GCAATGGTAC AGTCTGTGAG GATCTTGATG AGTGTATAGC TGTGTCAGAT 
GTTTGCTTTA AGGTGAATCA GGTCCATCGC TGTGTGAACA CAAATCCGGG TTTCCATTGC 
TTGCCATGTC CTCCGCGTTA TAAAGGAAGT CAGCCTTACG GAGTAGGGCT GGAAGTTGCC 
AAGACAGAGA AGCAAGTCTG TGAACCTGAG AATCCATGCA AAGACAAGAC ACACAGCTGC 
CATAAATCTG CAGAGTGCAT TTATCTGGGT CATTTCAGTG ATCCTATGTA CAAATGTGAA 
TGTAGGACAG GTTATGCTGG TGATGGACGC ATCTGTGGTG AGGATTCAGA TTTGGATGGA 
TGGCCAAATA ATAACTTGGT TTGTGCTGCT AATGCTACGT ATCACTGTGT GAAGGATAAC 
TGCCCCCTCC TGCCTAACTC TGGCCAAGAA GACTTTGATA AAGATGGCAA AGGAGATGCC 
TGTGATGAGG ATGATGACAA TGATGGTGTT GAAGATGACA AAGACAATTG CCCTCTTCTG 
TTCAATCCTC GTCAGTTTGA TTATGACAAA GATGAAGTTG GTGACCGCTG TGATAATTGC 
CCTTATGTGC ACAACCCCGC ACAGATTGAC ACAGATAATA ATGGGGAGGG TGATTCATGT 
GCTGTGGATA TTGATGGAGA TGACATTTTT AATGAACGAG ATAATTGTCC TTACGTTTAT 
AATACAGATC AGAGTGATAC AGATGGTGAT GGAGTTGGTG ATCAGTGTGA TAACTGTCCG 
CTAATGCATA ATCCAGACCA GACTGATGCA GATAACGATC TTGTTGGTGA CCAATGTGAC 
AACAATGAGG ACATAGATGA AGATGGCCAC CAGAACAACC AAGATAACTG CCCTTACATC 
CCCAATGCTA ACCAGGCTGA CCATGATAAA GATGGTAAAG GGGATGCCTG TGATCCTGAT 
GATGACAATG ATGGTATTCC AGATGACAGG GACAATTGTC GCCTCAGATA CAACCCTGAG 
CAGGAGGACT CTGATGGTGA TGGCAGAGGT GATATTTGCA AAGATGACTT TGACGATGAC 
AATGTTCCAG ATATTTTTGA TGTATGTCCT GAAAACAATG CCATCAGTGA AACTGATTTC 
AGAAAGTTCC AGATGGTCCC TCTGGACCCC AAGGGAACAG CTCAGATTGA TCCCAACTGG 
GTTATTCGCC ACCAAGGCAA GGAACTGGTG CAGACTGCCA ACTCTGATCC TGGAATAGCT 
GTAGGTTATG ATGAGTTCAG CTCTGTTGAC TTCAGTGGCA CATTCTATGT TAACACAGAC 
CGTGATGATG ACTATGCTGG CTTTGTTTTT GGCTACCAGT CCAGCAGCCG TTTTTATGTT 
CTAATGTGGA AGCAGGTCAC TCAAACCTAC TGGGAGGACA AACCCACGAG GGCTTATGGC 
TATTCTGGTG TGTCACTCAA GGTAGTGAAT TCAACAACTG GTACTGGGGA ACACTTGCGA 
AATGCTCTTT GGCATACAGG AAACACCCCC GGACAGGTGC GCACTTTATG GCATGATCCT 
AAGAACATTG GCTGGAAAGA TTATACTGCA TACAGGTGGC ATTTGATTCA CAGGCCTAAA 
ACAGGACTAA TAAAAGTTCT AGTGTATGAA GGGAAGCAAG TCATGGTGGA TTCTGGACCA 
ATCTATGATA CAACCTTTGC TGGTGGACGA TTAGGTCTAT TTGTCTTCTC TCAAGAAATG 
GTCTATTTCT CTGACCTCAA ATATGAATGC AGAGATGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS2 ( XM_015284051.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139537.1 Gallus gallus thrombospondin 2 (THBS2), transcript variant X1, mRNA.

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CloneID	OGa33580
Clone ID Related Accession (Same CDS sequence)	XM_015284051.2 , XM_015284051.1
Accession Version	XM_015284051.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	thrombospondin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGACG GCGGTAGGGC GCCGCGATCC GCTCCGCAAC GCCGGCAGCG CCCCGGGAGC 
AGGCAGAGCC CTCTCGTCCC GTCGGCTCTC CTTATGCCAG GAGTTATGCT GCAGAGGAGC 
AGGCTGTTGT GGCTTGCTGT CTTCATAACC CTGTGGGTTT CCTCAGATGC TCAGGATGAT 
GCCAAGGAAG AGGAGAATAC CTTTGATTTA CTTCAAATCA GTAACATAAA CCGAAAGACA 
ATTGGAGCAA AATTGTTTCG GGGTCCAGAT CCCGCTATCC CAGCATATCG TTTCATTCGG 
TTTGACCATA TACCTCCATT TAAACCAGAG AAGTTAAAAA AGATTGTTAA ACTCATACGG 
CAAAATGAGG GCTTTATCCT TTCAGCCACC CTGAGACAGG ACAGGCAATC TAGAGGCACT 
ATCCTTGCTT TAGAGGGTCC TGGCATCAGT GAGAGGCAGT TTGAGATAAT TTCCAATGGC 
CGAGCCAACA CCCTGGACCT CATTTATTGG GTGGATGGCT TCCAGAACGT GATTTCCCTG 
GAAGACGTGG ACCTTGCTGA CTCGCAGTGG AAGAATCTCA CCGTGCAAGT AACAGGAGAG 
AACTACAACC TCTATGTTGG CTGTGACCTT ATTGACAGCT TCATCCTGGA GGAGCCTTTC 
TATGAGCAAC TGAAAGCAGA GAACAGCAGA ATGTATGTGG CGAAAGGGTC CATTCGGGAG 
AATCATTTCA GGGGTCTTTT GCAAAATATT CATTTAATCT TTGATACTTC AATAGAGGAT 
GTTCTACGTA AAAAAGGATG CCAGAGGAGC CAATCAACTG AAGTGAACAC CATCAATGAG 
AGCACTGAGA TCCTTCACTT GAGCCCTGCT GTCACAACTG AGTATGTAGG TGAAAAGACA 
GAGAAGAAGG CTGAGTTCTG TGACCGCTCT TGTGAAGAGC TGGGAACAAT GTTCACTGAA 
CTCACTGGCC TGCGCATTGT GGTGAACAAT TTAGCTGATA ACCTCCAGAA AGTGTCTGAA 
GAGAATCAAA TAATGTGGGA ATTGATTGGC CCTAACAAAA CACTCAAGAA CCAATCAGTT 
TGCTGGCAGG ATGGCCGAGT CTTTGCAGAT AGTGAAAGCT GGATTGTAGA TAGCTGCACA 
AAGTGCACCT GCCAGGACTC TAAAATTGTG TGCCATCAAA TCACCTGTCC ACCAGTTTCT 
TGTGCTGATC CGTCCTTTAT TGAAGGTGAA TGCTGTCCAG TCTGCTCTCA CTCTGATGAT 
AGTGAGGAAG GCTGGTCACC CTGGTCAGAC TGGACAAAGT GCTCAGTTAC CTGTGGCTCT 
GGGACACAGA TGAGAGGAAG GTCTTGTGAT GTCACCAGGA GTGCTTGTAC AGGCCCACAC 
ATTCAGACGA GAATGTGCAG CTTCAAGAAA TGTGACCATC GCATACGTCA AGATGGTGGT 
TGGAGTCACT GGTCTCCCTG GTCCTCATGT TCAGTTACCT GTGGAGTAGG AAACATCACC 
CGCATCCGCC TCTGCAATTC CCCTATTCCC CAGATGGGTG GGAAAAACTG TGTGGGAAAT 
GGACGTGAAA CAGAGAAATG TGAGAAGGCC CCGTGCCCAG TGAATGGCCA GTGGGGTCCT 
TGGTCTCCAT GGTCTGCTTG CACTGTTACC TGTGGAGGAG GAATCCGTGA GAGGTCTCGC 
CTCTGTAACA GTCCAGAGCC ACAGTATGGA GGAAAGCCGT GTGTGGGAGA TACCAAGCAA 
CATGATATGT GCAATAAAAG GGATTGCCCA ATTGATGGTT GTTTGTCAAA TCCTTGCTTT 
CCTGGAGCAG AATGCAACAG TTACCCGGAT GGATCGTGGT CATGTGGGCC ATGCCCAGCA 
GGTTTCCTTG GCAATGGTAC AGTCTGTGAG GATCTTGATG AGTGTATAGC TGTGTCAGAT 
GTTTGCTTTA AGGTGAATCA GGTCCATCGC TGTGTGAACA CAAATCCGGG TTTCCATTGC 
TTGCCATGTC CTCCGCGTTA TAAAGGAAGT CAGCCTTACG GAGTAGGGCT GGAAGTTGCC 
AAGACAGAGA AGCAAGTCTG TGAACCTGAG AATCCATGCA AAGACAAGAC ACACAGCTGC 
CATAAATCTG CAGAGTGCAT TTATCTGGGT CATTTCAGTG ATCCTATGTA CAAATGTGAA 
TGTAGGACAG GTTATGCTGG TGATGGACGC ATCTGTGGTG AGGATTCAGA TTTGGATGGA 
TGGCCAAATA ATAACTTGGT TTGTGCTGCT AATGCTACGT ATCACTGTGT GAAGGATAAC 
TGCCCCCTCC TGCCTAACTC TGGCCAAGAA GACTTTGATA AAGATGGCAA AGGAGATGCC 
TGTGATGAGG ATGATGACAA TGATGGTGTT GAAGATGACA AAGACAATTG CCCTCTTCTG 
TTCAATCCTC GTCAGTTTGA TTATGACAAA GATGAAGTTG GTGACCGCTG TGATAATTGC 
CCTTATGTGC ACAACCCCGC ACAGATTGAC ACAGATAATA ATGGGGAGGG TGATTCATGT 
GCTGTGGATA TTGATGGAGA TGACATTTTT AATGAACGAG ATAATTGTCC TTACGTTTAT 
AATACAGATC AGAGTGATAC AGATGGTGAT GGAGTTGGTG ATCAGTGTGA TAACTGTCCG 
CTAATGCATA ATCCAGACCA GACTGATGCA GATAACGATC TTGTTGGTGA CCAATGTGAC 
AACAATGAGG ACATAGATGA AGATGGCCAC CAGAACAACC AAGATAACTG CCCTTACATC 
CCCAATGCTA ACCAGGCTGA CCATGATAAA GATGGTAAAG GGGATGCCTG TGATCCTGAT 
GATGACAATG ATGGTATTCC AGATGACAGG GACAATTGTC GCCTCAGATA CAACCCTGAG 
CAGGAGGACT CTGATGGTGA TGGCAGAGGT GATATTTGCA AAGATGACTT TGACGATGAC 
AATGTTCCAG ATATTTTTGA TGTATGTCCT GAAAACAATG CCATCAGTGA AACTGATTTC 
AGAAAGTTCC AGATGGTCCC TCTGGACCCC AAGGGAACAG CTCAGATTGA TCCCAACTGG 
GTTATTCGCC ACCAAGGCAA GGAACTGGTG CAGACTGCCA ACTCTGATCC TGGAATAGCT 
GTAGGTTATG ATGAGTTCAG CTCTGTTGAC TTCAGTGGCA CATTCTATGT TAACACAGAC 
CGTGATGATG ACTATGCTGG CTTTGTTTTT GGCTACCAGT CCAGCAGCCG TTTTTATGTT 
CTAATGTGGA AGCAGGTCAC TCAAACCTAC TGGGAGGACA AACCCACGAG GGCTTATGGC 
TATTCTGGTG TGTCACTCAA GGTAGTGAAT TCAACAACTG GTACTGGGGA ACACTTGCGA 
AATGCTCTTT GGCATACAGG AAACACCCCC GGACAGGTGC GCACTTTATG GCATGATCCT 
AAGAACATTG GCTGGAAAGA TTATACTGCA TACAGGTGGC ATTTGATTCA CAGGCCTAAA 
ACAGGACTAA TAAAAGTTCT AGTGTATGAA GGGAAGCAAG TCATGGTGGA TTCTGGACCA 
ATCTATGATA CAACCTTTGC TGGTGGACGA TTAGGTCTAT TTGTCTTCTC TCAAGAAATG 
GTCTATTTCT CTGACCTCAA ATATGAATGC AGAGATGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139537.1
CDS	98..3739
Translation	MCDGGRAPRSAPQRRQRPGSRQSPLVPSALLMPGVMLQRSRLLWLAVFITLWVSSDAQDDAKEEENTFDLLQISNINRKTIGAKLFRGPDPAIPAYRFIRFDHIPPFKPEKLKKIVKLIRQNEGFILSATLRQDRQSRGTILALEGPGISERQFEIISNGRANTLDLIYWVDGFQNVISLEDVDLADSQWKNLTVQVTGENYNLYVGCDLIDSFILEEPFYEQLKAENSRMYVAKGSIRENHFRGLLQNIHLIFDTSIEDVLRKKGCQRSQSTEVNTINESTEILHLSPAVTTEYVGEKTEKKAEFCDRSCEELGTMFTELTGLRIVVNNLADNLQKVSEENQIMWELIGPNKTLKNQSVCWQDGRVFADSESWIVDSCTKCTCQDSKIVCHQITCPPVSCADPSFIEGECCPVCSHSDDSEEGWSPWSDWTKCSVTCGSGTQMRGRSCDVTRSACTGPHIQTRMCSFKKCDHRIRQDGGWSHWSPWSSCSVTCGVGNITRIRLCNSPIPQMGGKNCVGNGRETEKCEKAPCPVNGQWGPWSPWSACTVTCGGGIRERSRLCNSPEPQYGGKPCVGDTKQHDMCNKRDCPIDGCLSNPCFPGAECNSYPDGSWSCGPCPAGFLGNGTVCEDLDECIAVSDVCFKVNQVHRCVNTNPGFHCLPCPPRYKGSQPYGVGLEVAKTEKQVCEPENPCKDKTHSCHKSAECIYLGHFSDPMYKCECRTGYAGDGRICGEDSDLDGWPNNNLVCAANATYHCVKDNCPLLPNSGQEDFDKDGKGDACDEDDDNDGVEDDKDNCPLLFNPRQFDYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDSCAVDIDGDDIFNERDNCPYVYNTDQSDTDGDGVGDQCDNCPLMHNPDQTDADNDLVGDQCDNNEDIDEDGHQNNQDNCPYIPNANQADHDKDGKGDACDPDDDNDGIPDDRDNCRLRYNPEQEDSDGDGRGDICKDDFDDDNVPDIFDVCPENNAISETDFRKFQMVPLDPKGTAQIDPNWVIRHQGKELVQTANSDPGIAVGYDEFSSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVLMWKQVTQTYWEDKPTRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTPGQVRTLWHDPKNIGWKDYTAYRWHLIHRPKTGLIKVLVYEGKQVMVDSGPIYDTTFAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	XM_015284051.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thrombospondin 2 (THBS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284051.1

ORF Insert Sequence:

ATGTGCGACG GCGGTAGGGC GCCGCGATCC GCTCCGCAAC GCCGGCAGCG CCCCGGGAGC 
AGGCAGAGCC CTCTCGTCCC GTCGGCTCTC CTTATGCCAG GAGTTATGCT GCAGAGGAGC 
AGGCTGTTGT GGCTTGCTGT CTTCATAACC CTGTGGGTTT CCTCAGATGC TCAGGATGAT 
GCCAAGGAAG AGGAGAATAC CTTTGATTTA CTTCAAATCA GTAACATAAA CCGAAAGACA 
ATTGGAGCAA AATTGTTTCG GGGTCCAGAT CCCGCTATCC CAGCATATCG TTTCATTCGG 
TTTGACCATA TACCTCCATT TAAACCAGAG AAGTTAAAAA AGATTGTTAA ACTCATACGG 
CAAAATGAGG GCTTTATCCT TTCAGCCACC CTGAGACAGG ACAGGCAATC TAGAGGCACT 
ATCCTTGCTT TAGAGGGTCC TGGCATCAGT GAGAGGCAGT TTGAGATAAT TTCCAATGGC 
CGAGCCAACA CCCTGGACCT CATTTATTGG GTGGATGGCT TCCAGAACGT GATTTCCCTG 
GAAGACGTGG ACCTTGCTGA CTCGCAGTGG AAGAATCTCA CCGTGCAAGT AACAGGAGAG 
AACTACAACC TCTATGTTGG CTGTGACCTT ATTGACAGCT TCATCCTGGA GGAGCCTTTC 
TATGAGCAAC TGAAAGCAGA GAACAGCAGA ATGTATGTGG CGAAAGGGTC CATTCGGGAG 
AATCATTTCA GGGGTCTTTT GCAAAATATT CATTTAATCT TTGATACTTC AATAGAGGAT 
GTTCTACGTA AAAAAGGATG CCAGAGGAGC CAATCAACTG AAGTGAACAC CATCAATGAG 
AGCACTGAGA TCCTTCACTT GAGCCCTGCT GTCACAACTG AGTATGTAGG TGAAAAGACA 
GAGAAGAAGG CTGAGTTCTG TGACCGCTCT TGTGAAGAGC TGGGAACAAT GTTCACTGAA 
CTCACTGGCC TGCGCATTGT GGTGAACAAT TTAGCTGATA ACCTCCAGAA AGTGTCTGAA 
GAGAATCAAA TAATGTGGGA ATTGATTGGC CCTAACAAAA CACTCAAGAA CCAATCAGTT 
TGCTGGCAGG ATGGCCGAGT CTTTGCAGAT AGTGAAAGCT GGATTGTAGA TAGCTGCACA 
AAGTGCACCT GCCAGGACTC TAAAATTGTG TGCCATCAAA TCACCTGTCC ACCAGTTTCT 
TGTGCTGATC CGTCCTTTAT TGAAGGTGAA TGCTGTCCAG TCTGCTCTCA CTCTGATGAT 
AGTGAGGAAG GCTGGTCACC CTGGTCAGAC TGGACAAAGT GCTCAGTTAC CTGTGGCTCT 
GGGACACAGA TGAGAGGAAG GTCTTGTGAT GTCACCAGGA GTGCTTGTAC AGGCCCACAC 
ATTCAGACGA GAATGTGCAG CTTCAAGAAA TGTGACCATC GCATACGTCA AGATGGTGGT 
TGGAGTCACT GGTCTCCCTG GTCCTCATGT TCAGTTACCT GTGGAGTAGG AAACATCACC 
CGCATCCGCC TCTGCAATTC CCCTATTCCC CAGATGGGTG GGAAAAACTG TGTGGGAAAT 
GGACGTGAAA CAGAGAAATG TGAGAAGGCC CCGTGCCCAG TGAATGGCCA GTGGGGTCCT 
TGGTCTCCAT GGTCTGCTTG CACTGTTACC TGTGGAGGAG GAATCCGTGA GAGGTCTCGC 
CTCTGTAACA GTCCAGAGCC ACAGTATGGA GGAAAGCCGT GTGTGGGAGA TACCAAGCAA 
CATGATATGT GCAATAAAAG GGATTGCCCA ATTGATGGTT GTTTGTCAAA TCCTTGCTTT 
CCTGGAGCAG AATGCAACAG TTACCCGGAT GGATCGTGGT CATGTGGGCC ATGCCCAGCA 
GGTTTCCTTG GCAATGGTAC AGTCTGTGAG GATCTTGATG AGTGTATAGC TGTGTCAGAT 
GTTTGCTTTA AGGTGAATCA GGTCCATCGC TGTGTGAACA CAAATCCGGG TTTCCATTGC 
TTGCCATGTC CTCCGCGTTA TAAAGGAAGT CAGCCTTACG GAGTAGGGCT GGAAGTTGCC 
AAGACAGAGA AGCAAGTCTG TGAACCTGAG AATCCATGCA AAGACAAGAC ACACAGCTGC 
CATAAATCTG CAGAGTGCAT TTATCTGGGT CATTTCAGTG ATCCTATGTA CAAATGTGAA 
TGTAGGACAG GTTATGCTGG TGATGGACGC ATCTGTGGTG AGGATTCAGA TTTGGATGGA 
TGGCCAAATA ATAACTTGGT TTGTGCTGCT AATGCTACGT ATCACTGTGT GAAGGATAAC 
TGCCCCCTCC TGCCTAACTC TGGCCAAGAA GACTTTGATA AAGATGGCAA AGGAGATGCC 
TGTGATGAGG ATGATGACAA TGATGGTGTT GAAGATGACA AAGACAATTG CCCTCTTCTG 
TTCAATCCTC GTCAGTTTGA TTATGACAAA GATGAAGTTG GTGACCGCTG TGATAATTGC 
CCTTATGTGC ACAACCCCGC ACAGATTGAC ACAGATAATA ATGGGGAGGG TGATTCATGT 
GCTGTGGATA TTGATGGAGA TGACATTTTT AATGAACGAG ATAATTGTCC TTACGTTTAT 
AATACAGATC AGAGTGATAC AGATGGTGAT GGAGTTGGTG ATCAGTGTGA TAACTGTCCG 
CTAATGCATA ATCCAGACCA GACTGATGCA GATAACGATC TTGTTGGTGA CCAATGTGAC 
AACAATGAGG ACATAGATGA AGATGGCCAC CAGAACAACC AAGATAACTG CCCTTACATC 
CCCAATGCTA ACCAGGCTGA CCATGATAAA GATGGTAAAG GGGATGCCTG TGATCCTGAT 
GATGACAATG ATGGTATTCC AGATGACAGG GACAATTGTC GCCTCAGATA CAACCCTGAG 
CAGGAGGACT CTGATGGTGA TGGCAGAGGT GATATTTGCA AAGATGACTT TGACGATGAC 
AATGTTCCAG ATATTTTTGA TGTATGTCCT GAAAACAATG CCATCAGTGA AACTGATTTC 
AGAAAGTTCC AGATGGTCCC TCTGGACCCC AAGGGAACAG CTCAGATTGA TCCCAACTGG 
GTTATTCGCC ACCAAGGCAA GGAACTGGTG CAGACTGCCA ACTCTGATCC TGGAATAGCT 
GTAGGTTATG ATGAGTTCAG CTCTGTTGAC TTCAGTGGCA CATTCTATGT TAACACAGAC 
CGTGATGATG ACTATGCTGG CTTTGTTTTT GGCTACCAGT CCAGCAGCCG TTTTTATGTT 
CTAATGTGGA AGCAGGTCAC TCAAACCTAC TGGGAGGACA AACCCACGAG GGCTTATGGC 
TATTCTGGTG TGTCACTCAA GGTAGTGAAT TCAACAACTG GTACTGGGGA ACACTTGCGA 
AATGCTCTTT GGCATACAGG AAACACCCCC GGACAGGTGC GCACTTTATG GCATGATCCT 
AAGAACATTG GCTGGAAAGA TTATACTGCA TACAGGTGGC ATTTGATTCA CAGGCCTAAA 
ACAGGACTAA TAAAAGTTCT AGTGTATGAA GGGAAGCAAG TCATGGTGGA TTCTGGACCA 
ATCTATGATA CAACCTTTGC TGGTGGACGA TTAGGTCTAT TTGTCTTCTC TCAAGAAATG 
GTCTATTTCT CTGACCTCAA ATATGAATGC AGAGATGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS2 ( XM_015284053.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139539.1 Gallus gallus thrombospondin 2 (THBS2), transcript variant X2, mRNA.

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CloneID	OGa33581
Clone ID Related Accession (Same CDS sequence)	XM_015284053.1
Accession Version	XM_015284053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	thrombospondin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGA GGAGCAGGCT GTTGTGGCTT GCTGTCTTCA TAACCCTGTG GGTTTCCTCA 
GATGCTCAGG ATGATGCCAA GGAAGAGGAG AATACCTTTG ATTTACTTCA AATCAGTAAC 
ATAAACCGAA AGACAATTGG AGCAAAATTG TTTCGGGGTC CAGATCCCGC TATCCCAGCA 
TATCGTTTCA TTCGGTTTGA CCATATACCT CCATTTAAAC CAGAGAAGTT AAAAAAGATT 
GTTAAACTCA TACGGCAAAA TGAGGGCTTT ATCCTTTCAG CCACCCTGAG ACAGGACAGG 
CAATCTAGAG GCACTATCCT TGCTTTAGAG GGTCCTGGCA TCAGTGAGAG GCAGTTTGAG 
ATAATTTCCA ATGGCCGAGC CAACACCCTG GACCTCATTT ATTGGGTGGA TGGCTTCCAG 
AACGTGATTT CCCTGGAAGA CGTGGACCTT GCTGACTCGC AGTGGAAGAA TCTCACCGTG 
CAAGTAACAG GAGAGAACTA CAACCTCTAT GTTGGCTGTG ACCTTATTGA CAGCTTCATC 
CTGGAGGAGC CTTTCTATGA GCAACTGAAA GCAGAGAACA GCAGAATGTA TGTGGCGAAA 
GGGTCCATTC GGGAGAATCA TTTCAGGGGT CTTTTGCAAA ATATTCATTT AATCTTTGAT 
ACTTCAATAG AGGATGTTCT ACGTAAAAAA GGATGCCAGA GGAGCCAATC AACTGAAGTG 
AACACCATCA ATGAGAGCAC TGAGATCCTT CACTTGAGCC CTGCTGTCAC AACTGAGTAT 
GTAGGTGAAA AGACAGAGAA GAAGGCTGAG TTCTGTGACC GCTCTTGTGA AGAGCTGGGA 
ACAATGTTCA CTGAACTCAC TGGCCTGCGC ATTGTGGTGA ACAATTTAGC TGATAACCTC 
CAGAAAGTGT CTGAAGAGAA TCAAATAATG TGGGAATTGA TTGGCCCTAA CAAAACACTC 
AAGAACCAAT CAGTTTGCTG GCAGGATGGC CGAGTCTTTG CAGATAGTGA AAGCTGGATT 
GTAGATAGCT GCACAAAGTG CACCTGCCAG GACTCTAAAA TTGTGTGCCA TCAAATCACC 
TGTCCACCAG TTTCTTGTGC TGATCCGTCC TTTATTGAAG GTGAATGCTG TCCAGTCTGC 
TCTCACTCTG ATGATAGTGA GGAAGGCTGG TCACCCTGGT CAGACTGGAC AAAGTGCTCA 
GTTACCTGTG GCTCTGGGAC ACAGATGAGA GGAAGGTCTT GTGATGTCAC CAGGAGTGCT 
TGTACAGGCC CACACATTCA GACGAGAATG TGCAGCTTCA AGAAATGTGA CCATCGCATA 
CGTCAAGATG GTGGTTGGAG TCACTGGTCT CCCTGGTCCT CATGTTCAGT TACCTGTGGA 
GTAGGAAACA TCACCCGCAT CCGCCTCTGC AATTCCCCTA TTCCCCAGAT GGGTGGGAAA 
AACTGTGTGG GAAATGGACG TGAAACAGAG AAATGTGAGA AGGCCCCGTG CCCAGTGAAT 
GGCCAGTGGG GTCCTTGGTC TCCATGGTCT GCTTGCACTG TTACCTGTGG AGGAGGAATC 
CGTGAGAGGT CTCGCCTCTG TAACAGTCCA GAGCCACAGT ATGGAGGAAA GCCGTGTGTG 
GGAGATACCA AGCAACATGA TATGTGCAAT AAAAGGGATT GCCCAATTGA TGGTTGTTTG 
TCAAATCCTT GCTTTCCTGG AGCAGAATGC AACAGTTACC CGGATGGATC GTGGTCATGT 
GGGCCATGCC CAGCAGGTTT CCTTGGCAAT GGTACAGTCT GTGAGGATCT TGATGAGTGT 
ATAGCTGTGT CAGATGTTTG CTTTAAGGTG AATCAGGTCC ATCGCTGTGT GAACACAAAT 
CCGGGTTTCC ATTGCTTGCC ATGTCCTCCG CGTTATAAAG GAAGTCAGCC TTACGGAGTA 
GGGCTGGAAG TTGCCAAGAC AGAGAAGCAA GTCTGTGAAC CTGAGAATCC ATGCAAAGAC 
AAGACACACA GCTGCCATAA ATCTGCAGAG TGCATTTATC TGGGTCATTT CAGTGATCCT 
ATGTACAAAT GTGAATGTAG GACAGGTTAT GCTGGTGATG GACGCATCTG TGGTGAGGAT 
TCAGATTTGG ATGGATGGCC AAATAATAAC TTGGTTTGTG CTGCTAATGC TACGTATCAC 
TGTGTGAAGG ATAACTGCCC CCTCCTGCCT AACTCTGGCC AAGAAGACTT TGATAAAGAT 
GGCAAAGGAG ATGCCTGTGA TGAGGATGAT GACAATGATG GTGTTGAAGA TGACAAAGAC 
AATTGCCCTC TTCTGTTCAA TCCTCGTCAG TTTGATTATG ACAAAGATGA AGTTGGTGAC 
CGCTGTGATA ATTGCCCTTA TGTGCACAAC CCCGCACAGA TTGACACAGA TAATAATGGG 
GAGGGTGATT CATGTGCTGT GGATATTGAT GGAGATGACA TTTTTAATGA ACGAGATAAT 
TGTCCTTACG TTTATAATAC AGATCAGAGT GATACAGATG GTGATGGAGT TGGTGATCAG 
TGTGATAACT GTCCGCTAAT GCATAATCCA GACCAGACTG ATGCAGATAA CGATCTTGTT 
GGTGACCAAT GTGACAACAA TGAGGACATA GATGAAGATG GCCACCAGAA CAACCAAGAT 
AACTGCCCTT ACATCCCCAA TGCTAACCAG GCTGACCATG ATAAAGATGG TAAAGGGGAT 
GCCTGTGATC CTGATGATGA CAATGATGGT ATTCCAGATG ACAGGGACAA TTGTCGCCTC 
AGATACAACC CTGAGCAGGA GGACTCTGAT GGTGATGGCA GAGGTGATAT TTGCAAAGAT 
GACTTTGACG ATGACAATGT TCCAGATATT TTTGATGTAT GTCCTGAAAA CAATGCCATC 
AGTGAAACTG ATTTCAGAAA GTTCCAGATG GTCCCTCTGG ACCCCAAGGG AACAGCTCAG 
ATTGATCCCA ACTGGGTTAT TCGCCACCAA GGCAAGGAAC TGGTGCAGAC TGCCAACTCT 
GATCCTGGAA TAGCTGTAGG TTATGATGAG TTCAGCTCTG TTGACTTCAG TGGCACATTC 
TATGTTAACA CAGACCGTGA TGATGACTAT GCTGGCTTTG TTTTTGGCTA CCAGTCCAGC 
AGCCGTTTTT ATGTTCTAAT GTGGAAGCAG GTCACTCAAA CCTACTGGGA GGACAAACCC 
ACGAGGGCTT ATGGCTATTC TGGTGTGTCA CTCAAGGTAG TGAATTCAAC AACTGGTACT 
GGGGAACACT TGCGAAATGC TCTTTGGCAT ACAGGAAACA CCCCCGGACA GGTGCGCACT 
TTATGGCATG ATCCTAAGAA CATTGGCTGG AAAGATTATA CTGCATACAG GTGGCATTTG 
ATTCACAGGC CTAAAACAGG ACTAATAAAA GTTCTAGTGT ATGAAGGGAA GCAAGTCATG 
GTGGATTCTG GACCAATCTA TGATACAACC TTTGCTGGTG GACGATTAGG TCTATTTGTC 
TTCTCTCAAG AAATGGTCTA TTTCTCTGAC CTCAAATATG AATGCAGAGA TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139539.1
CDS	24..3560
Translation	MLQRSRLLWLAVFITLWVSSDAQDDAKEEENTFDLLQISNINRKTIGAKLFRGPDPAIPAYRFIRFDHIPPFKPEKLKKIVKLIRQNEGFILSATLRQDRQSRGTILALEGPGISERQFEIISNGRANTLDLIYWVDGFQNVISLEDVDLADSQWKNLTVQVTGENYNLYVGCDLIDSFILEEPFYEQLKAENSRMYVAKGSIRENHFRGLLQNIHLIFDTSIEDVLRKKGCQRSQSTEVNTINESTEILHLSPAVTTEYVGEKTEKKAEFCDRSCEELGTMFTELTGLRIVVNNLADNLQKVSEENQIMWELIGPNKTLKNQSVCWQDGRVFADSESWIVDSCTKCTCQDSKIVCHQITCPPVSCADPSFIEGECCPVCSHSDDSEEGWSPWSDWTKCSVTCGSGTQMRGRSCDVTRSACTGPHIQTRMCSFKKCDHRIRQDGGWSHWSPWSSCSVTCGVGNITRIRLCNSPIPQMGGKNCVGNGRETEKCEKAPCPVNGQWGPWSPWSACTVTCGGGIRERSRLCNSPEPQYGGKPCVGDTKQHDMCNKRDCPIDGCLSNPCFPGAECNSYPDGSWSCGPCPAGFLGNGTVCEDLDECIAVSDVCFKVNQVHRCVNTNPGFHCLPCPPRYKGSQPYGVGLEVAKTEKQVCEPENPCKDKTHSCHKSAECIYLGHFSDPMYKCECRTGYAGDGRICGEDSDLDGWPNNNLVCAANATYHCVKDNCPLLPNSGQEDFDKDGKGDACDEDDDNDGVEDDKDNCPLLFNPRQFDYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDSCAVDIDGDDIFNERDNCPYVYNTDQSDTDGDGVGDQCDNCPLMHNPDQTDADNDLVGDQCDNNEDIDEDGHQNNQDNCPYIPNANQADHDKDGKGDACDPDDDNDGIPDDRDNCRLRYNPEQEDSDGDGRGDICKDDFDDDNVPDIFDVCPENNAISETDFRKFQMVPLDPKGTAQIDPNWVIRHQGKELVQTANSDPGIAVGYDEFSSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVLMWKQVTQTYWEDKPTRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTPGQVRTLWHDPKNIGWKDYTAYRWHLIHRPKTGLIKVLVYEGKQVMVDSGPIYDTTFAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	XM_015284053.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thrombospondin 2 (THBS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284053.1

ORF Insert Sequence:

ATGCTGCAGA GGAGCAGGCT GTTGTGGCTT GCTGTCTTCA TAACCCTGTG GGTTTCCTCA 
GATGCTCAGG ATGATGCCAA GGAAGAGGAG AATACCTTTG ATTTACTTCA AATCAGTAAC 
ATAAACCGAA AGACAATTGG AGCAAAATTG TTTCGGGGTC CAGATCCCGC TATCCCAGCA 
TATCGTTTCA TTCGGTTTGA CCATATACCT CCATTTAAAC CAGAGAAGTT AAAAAAGATT 
GTTAAACTCA TACGGCAAAA TGAGGGCTTT ATCCTTTCAG CCACCCTGAG ACAGGACAGG 
CAATCTAGAG GCACTATCCT TGCTTTAGAG GGTCCTGGCA TCAGTGAGAG GCAGTTTGAG 
ATAATTTCCA ATGGCCGAGC CAACACCCTG GACCTCATTT ATTGGGTGGA TGGCTTCCAG 
AACGTGATTT CCCTGGAAGA CGTGGACCTT GCTGACTCGC AGTGGAAGAA TCTCACCGTG 
CAAGTAACAG GAGAGAACTA CAACCTCTAT GTTGGCTGTG ACCTTATTGA CAGCTTCATC 
CTGGAGGAGC CTTTCTATGA GCAACTGAAA GCAGAGAACA GCAGAATGTA TGTGGCGAAA 
GGGTCCATTC GGGAGAATCA TTTCAGGGGT CTTTTGCAAA ATATTCATTT AATCTTTGAT 
ACTTCAATAG AGGATGTTCT ACGTAAAAAA GGATGCCAGA GGAGCCAATC AACTGAAGTG 
AACACCATCA ATGAGAGCAC TGAGATCCTT CACTTGAGCC CTGCTGTCAC AACTGAGTAT 
GTAGGTGAAA AGACAGAGAA GAAGGCTGAG TTCTGTGACC GCTCTTGTGA AGAGCTGGGA 
ACAATGTTCA CTGAACTCAC TGGCCTGCGC ATTGTGGTGA ACAATTTAGC TGATAACCTC 
CAGAAAGTGT CTGAAGAGAA TCAAATAATG TGGGAATTGA TTGGCCCTAA CAAAACACTC 
AAGAACCAAT CAGTTTGCTG GCAGGATGGC CGAGTCTTTG CAGATAGTGA AAGCTGGATT 
GTAGATAGCT GCACAAAGTG CACCTGCCAG GACTCTAAAA TTGTGTGCCA TCAAATCACC 
TGTCCACCAG TTTCTTGTGC TGATCCGTCC TTTATTGAAG GTGAATGCTG TCCAGTCTGC 
TCTCACTCTG ATGATAGTGA GGAAGGCTGG TCACCCTGGT CAGACTGGAC AAAGTGCTCA 
GTTACCTGTG GCTCTGGGAC ACAGATGAGA GGAAGGTCTT GTGATGTCAC CAGGAGTGCT 
TGTACAGGCC CACACATTCA GACGAGAATG TGCAGCTTCA AGAAATGTGA CCATCGCATA 
CGTCAAGATG GTGGTTGGAG TCACTGGTCT CCCTGGTCCT CATGTTCAGT TACCTGTGGA 
GTAGGAAACA TCACCCGCAT CCGCCTCTGC AATTCCCCTA TTCCCCAGAT GGGTGGGAAA 
AACTGTGTGG GAAATGGACG TGAAACAGAG AAATGTGAGA AGGCCCCGTG CCCAGTGAAT 
GGCCAGTGGG GTCCTTGGTC TCCATGGTCT GCTTGCACTG TTACCTGTGG AGGAGGAATC 
CGTGAGAGGT CTCGCCTCTG TAACAGTCCA GAGCCACAGT ATGGAGGAAA GCCGTGTGTG 
GGAGATACCA AGCAACATGA TATGTGCAAT AAAAGGGATT GCCCAATTGA TGGTTGTTTG 
TCAAATCCTT GCTTTCCTGG AGCAGAATGC AACAGTTACC CGGATGGATC GTGGTCATGT 
GGGCCATGCC CAGCAGGTTT CCTTGGCAAT GGTACAGTCT GTGAGGATCT TGATGAGTGT 
ATAGCTGTGT CAGATGTTTG CTTTAAGGTG AATCAGGTCC ATCGCTGTGT GAACACAAAT 
CCGGGTTTCC ATTGCTTGCC ATGTCCTCCG CGTTATAAAG GAAGTCAGCC TTACGGAGTA 
GGGCTGGAAG TTGCCAAGAC AGAGAAGCAA GTCTGTGAAC CTGAGAATCC ATGCAAAGAC 
AAGACACACA GCTGCCATAA ATCTGCAGAG TGCATTTATC TGGGTCATTT CAGTGATCCT 
ATGTACAAAT GTGAATGTAG GACAGGTTAT GCTGGTGATG GACGCATCTG TGGTGAGGAT 
TCAGATTTGG ATGGATGGCC AAATAATAAC TTGGTTTGTG CTGCTAATGC TACGTATCAC 
TGTGTGAAGG ATAACTGCCC CCTCCTGCCT AACTCTGGCC AAGAAGACTT TGATAAAGAT 
GGCAAAGGAG ATGCCTGTGA TGAGGATGAT GACAATGATG GTGTTGAAGA TGACAAAGAC 
AATTGCCCTC TTCTGTTCAA TCCTCGTCAG TTTGATTATG ACAAAGATGA AGTTGGTGAC 
CGCTGTGATA ATTGCCCTTA TGTGCACAAC CCCGCACAGA TTGACACAGA TAATAATGGG 
GAGGGTGATT CATGTGCTGT GGATATTGAT GGAGATGACA TTTTTAATGA ACGAGATAAT 
TGTCCTTACG TTTATAATAC AGATCAGAGT GATACAGATG GTGATGGAGT TGGTGATCAG 
TGTGATAACT GTCCGCTAAT GCATAATCCA GACCAGACTG ATGCAGATAA CGATCTTGTT 
GGTGACCAAT GTGACAACAA TGAGGACATA GATGAAGATG GCCACCAGAA CAACCAAGAT 
AACTGCCCTT ACATCCCCAA TGCTAACCAG GCTGACCATG ATAAAGATGG TAAAGGGGAT 
GCCTGTGATC CTGATGATGA CAATGATGGT ATTCCAGATG ACAGGGACAA TTGTCGCCTC 
AGATACAACC CTGAGCAGGA GGACTCTGAT GGTGATGGCA GAGGTGATAT TTGCAAAGAT 
GACTTTGACG ATGACAATGT TCCAGATATT TTTGATGTAT GTCCTGAAAA CAATGCCATC 
AGTGAAACTG ATTTCAGAAA GTTCCAGATG GTCCCTCTGG ACCCCAAGGG AACAGCTCAG 
ATTGATCCCA ACTGGGTTAT TCGCCACCAA GGCAAGGAAC TGGTGCAGAC TGCCAACTCT 
GATCCTGGAA TAGCTGTAGG TTATGATGAG TTCAGCTCTG TTGACTTCAG TGGCACATTC 
TATGTTAACA CAGACCGTGA TGATGACTAT GCTGGCTTTG TTTTTGGCTA CCAGTCCAGC 
AGCCGTTTTT ATGTTCTAAT GTGGAAGCAG GTCACTCAAA CCTACTGGGA GGACAAACCC 
ACGAGGGCTT ATGGCTATTC TGGTGTGTCA CTCAAGGTAG TGAATTCAAC AACTGGTACT 
GGGGAACACT TGCGAAATGC TCTTTGGCAT ACAGGAAACA CCCCCGGACA GGTGCGCACT 
TTATGGCATG ATCCTAAGAA CATTGGCTGG AAAGATTATA CTGCATACAG GTGGCATTTG 
ATTCACAGGC CTAAAACAGG ACTAATAAAA GTTCTAGTGT ATGAAGGGAA GCAAGTCATG 
GTGGATTCTG GACCAATCTA TGATACAACC TTTGCTGGTG GACGATTAGG TCTATTTGTC 
TTCTCTCAAG AAATGGTCTA TTTCTCTGAC CTCAAATATG AATGCAGAGA TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cloning and sequencing of chicken thrombospondin.
The Journal of biological chemistry266(13)8039-43(1991 May)
Lawler J,Duquette M,Ferro P

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THBS2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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