Thbs2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thbs2
Entrez Gene ID	21826
Full Name	thrombospondin 2
Synonyms	TSP2,Thbs-2
General protein information	Preferred Names thrombospondin 2 Names thrombospondin-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A2\|17 8.95 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011581.3	NP_035711.2	thrombospondin-2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	THBS2	NP_003238.2
Macaca mulatta (Rhesus monkey)	THBS2	XP_001096616.2
Mus musculus (house mouse)	Thbs2	NP_035711.2
Gallus gallus (chicken)	THBS2	NP_001001755.1
Danio rerio (zebrafish)	thbs2b	XP_694690.6
Danio rerio (zebrafish)	LOC100535241	XP_003199793.2
Pan troglodytes (chimpanzee)	THBS2	XP_003311640.2
Canis lupus familiaris (dog)	THBS2	NP_001184085.1
Danio rerio (zebrafish)	thbs2a	XP_005157071.1
Bos taurus (cattle)	THBS2	NP_789861.1
Rattus norvegicus (Norway rat)	Thbs2	NP_001162609.1
Xenopus tropicalis (tropical clawed frog)	thbs2	XP_002931559.2

Gene Ontology
Bibliography

Related articles in PubMed

Molecular codes and in vitro generation of hypocretin and melanin concentrating hormone neurons.
Seifinejad A, Li S, Mikhail C, Vassalli A, Pradervand S, Arribat Y, Pezeshgi Modarres H, Allen B, John RM, Amati F, Tafti M
Proceedings of the National Academy of Sciences of the United States of America116(34)17061-17070(2019 Aug)

Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability.
Schips TG, Vanhoutte D, Vo A, Correll RN, Brody MJ, Khalil H, Karch J, Tjondrokoesoemo A, Sargent MA, Maillet M, Ross RS, Molkentin JD
Nature communications10(1)76(2019 01)

Myh10 deficiency leads to defective extracellular matrix remodeling and pulmonary disease.
Kim HT, Yin W, Jin YJ, Panza P, Gunawan F, Grohmann B, Buettner C, Sokol AM, Preussner J, Guenther S, Kostin S, Ruppert C, Bhagwat AM, Ma X, Graumann J, Looso M, Guenther A, Adelstein RS, Offermanns S, Stainier DYR
Nature communications9(1)4600(2018 11)

Sexually dimorphic behavior, neuronal activity, and gene expression in Chd8-mutant mice.
Jung H, Park H, Choi Y, Kang H, Lee E, Kweon H, Roh JD, Ellegood J, Choi W, Kang J, Rhim I, Choi SY, Bae M, Kim SG, Lee J, Chung C, Yoo T, Park H, Kim Y, Ha S, Um SM, Mo S, Kwon Y, Mah W, Bae YC, Kim H, Lerch JP, Paik SB, Kim E
Nature neuroscience21(9)1218-1228(2018 09)

MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2.
Wei WF, Zhou CF, Wu XG, He LN, Wu LF, Chen XJ, Yan RM, Zhong M, Yu YH, Liang L, Wang W
Cell death & disease8(12)3220(2017 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Thbs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thbs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16247	NM_011581.3 Latest version!	Mus musculus thrombospondin 2 (Thbs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thbs2 ( NM_011581.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035711.2 Mus musculus thrombospondin 2 (Thbs2), mRNA.

Price & Availability↑

CloneID	OMu16247
Clone ID Related Accession (Same CDS sequence)	NM_011581.3
Accession Version	NM_011581.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574697600000
Organism	Mus musculus(house mouse)
Product	thrombospondin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ049934.1, AK132508.1, AK142552.1 and AK165881.1. On Jun 11, 2009 this sequence version replaced NM_011581.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK142552.1, AK132508.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCTGGG CACTGGCCCT GCTGGCTCTG GGCATAGGGC CAAGAGCTTC TGCTGGTGAC 
CACGTCAAGG ACACTTCATT TGACCTTTTC AGCATCAGCA ACATTAACCG GAAGACCATC 
GGTGCCAAGC AGTTCCGAGG GCCTGACCCC GGGGTGCCCG CCTACCGTTT TGTACGGTTT 
GACTACATCC CCCCAGTGAA CACAGATGAT CTCAACAGGA TTGTCAAGCT TGCAAGGAGA 
AAGGAGGGCT TCTTCCTCAC AGCCCAACTG AAGCAGGACC GCAAGTCTCG GGGAACGCTC 
CTGGTGTTGG AAGGCCCCGG CACCTCCCAG AGGCAGTTTG AGATTGTGTC CAATGGCCCA 
GGGGACACTT TGGACCTCAA CTACTGGGTA GAAGGCAATC AGCATACCAA CTTCCTGGAG 
GATGTGGGCC TGGCTGACTC CCAGTGGAAG AATGTGACTG TGCAGGTGGC CAGTGACACC 
TATAGCCTGT ATGTGGGCTG CGATCTTATC GACAGTGTCA CCCTGGAAGA ACCATTCTAT 
GAGCAGCTAG AAGTAGACAG GAGCAGGATG TACGTGGCCA AAGGTGCATC TCGAGAGAGT 
CACTTCAGGG GCTTGCTGCA GAATGTCCAT CTCGTGTTTG CAGATTCTGT GGAAGATATC 
TTAAGCAAGA AAGGCTGTCA ACACAGCCAG GGAGCTGAAG TCAACACCAT CAGTGAACAT 
ACAGAGACTC TCCATCTGAG CCCTCACATC ACCACAGATC TCGTGGTCCA GGGTGTGGAG 
AAGGCACAGG AGGTGTGTAC GCACTCCTGC GAGGAGTTGA GCAACATGAT GAATGAGCTC 
TCTGGACTGC ACGTCATGGT GAACCAGCTG AGCAAGAACC TGGAGAGAGT GTCTAGTGAT 
AACCAGTTCC TTTTGGAGCT CATTGGGGGC CCTCTGAAGA CAAGAAACAT GTCAGCCTGT 
GTGCAGGAGG GCCGAATCTT TGCAGAAAAT GAAACCTGGG TTGTGGATAG TTGTACCACA 
TGCACCTGCA AGAAATTTAA AACAGTCTGC CATCAGATCA CCTGCTCACC TGCAACTTGT 
GCCAACCCAT CTTTTGTGGA AGGCGAGTGC TGTCCATCCT GTTCACACTC TGCAGACAGT 
GATGAGGGCT GGTCTCCGTG GGCAGAGTGG ACCGAGTGTT CTGTCACCTG TGGCTCTGGG 
ACCCAGCAGA GAGGCCGGTC TTGTGATGTC ACCAGCAACA CCTGCCTGGG CCCCTCCATT 
CAGACAAGGA CATGCAGCCT GGGCAAATGT GATACGAGAA TCCGTCAGAA TGGAGGCTGG 
AGTCACTGGT CACCCTGGTC TTCATGCTCC GTGACTTGTG GAGTTGGCAA TGTCACCCGC 
ATACGTCTCT GCAACTCACC AGTGCCCCAG ATGGGTGGCA AGAACTGCAA GGGCAGCGGC 
CGGGAAACCA AACCCTGTCA GCGTGATCCG TGCCCAATTG ATGGCCGCTG GAGCCCCTGG 
TCCCCTTGGT CAGCCTGCAC AGTTACCTGT GCTGGAGGGA TCCGTGAGCG CTCACGTGTT 
TGCAACAGCC CTGAGCCCCA GTATGGAGGG AAGGACTGTG TCGGGGATGT GACAGAACAC 
CAAATGTGCA ACAAGAGAAG CTGCCCTATT GATGGGTGCT TATCCAACCC GTGTTTTCCT 
GGAGCCAAGT GCAACAGCTT CCCTGATGGG TCCTGGTCCT GTGGCTCCTG CCCAGTGGGC 
TTTCTGGGCA ATGGTACCCA CTGTGAGGAC CTGGATGAGT GTGCTGTGGT CACAGATATT 
TGCTTCTCAA CTAACAAAGC TCCCCGCTGT GTCAACACCA ACCCGGGCTT CCACTGCCTG 
CCTTGTCCAC CACGCTACAA GGGGAACCAA CCCTTCGGTG TTGGCCTGGA GGATGCTAGG 
ACAGAAAAAC AAGTGTGTGA GCCAGAGAAT CCATGTAAGG ACAAGACTCA CAGCTGCCAC 
AAGAATGCAG AGTGCATCTA CCTGGGCCAC TTTAGTGACC CCATGTACAA GTGTGAGTGC 
CAGATTGGCT ACGCAGGTGA TGGGCTCATC TGCGGGGAGG ACTCAGACCT GGATGGCTGG 
CCCAACAACA ACCTGGTGTG TGCTACTAAT GCCACCTACC ACTGCATCAA GGACAACTGC 
CCCAAACTGC CAAATTCCGG GCAGGAGGAT TTTGATAAGG ATGGAATCGG AGATGCTTGT 
GACGAGGACG ATGACAATGA CGGTGTGAGC GATGAGAAGG ACAATTGCCA GCTTCTCTTC 
AATCCCCGTC AATTAGACTA TGACAAGGAT GAGGTTGGAG ACCGCTGTGA CAACTGCCCC 
TATGTGCACA ACCCAGCACA GATCGACACA GACAACAATG GCGAGGGGGA TGCCTGCTCT 
GTGGACATTG ACGGAGACGA TGTTTTCAAT GAGCGAGACA ATTGTCCATA TGTCTACAAC 
ACTGACCAGA GAGATACGGA TGGTGATGGC GTGGGTGACC ACTGTGACAA TTGTCCTCTG 
ATGCACAACC CAGATCAGAT CGATCAGGAC AATGATCTCG TTGGAGACCA GTGTGACAAC 
AATGAGGACA TAGATGATGA CGGCCACCAG AACAACCAAG ACAACTGCCC ATACATCTCC 
AACTCCAACC AGGCTGACCA TGACAACGAC GGCAAGGGCG ATGCCTGCGA CTCTGATGAT 
GACAATGATG GTGTTCCAGA TGACAGGGAC AACTGTCGGC TTGTGTTCAA CCCAGACCAG 
GAAGACTCGG ACGGTGACGG CCGAGGTGAC ATTTGTAAAG ATGACTTTGA CAATGATAAT 
GTCCCAGATA TTGATGATGT GTGCCCTGAG AACAATGCCA TCACTGAGAC AGACTTCAGA 
AACTTCCAGA TGGTCCCTCT GGATCCCAAG GGGACCACAC AAATTGATCC CAACTGGGTA 
ATTCGTCACC AAGGCAAAGA GCTGGTGCAG ACAGCAAACT CAGACCCTGG CATCGCTGTA 
GGTTTCGACG AGTTTGGGTC TGTGGACTTC AGTGGCACTT TCTATGTCAA CACTGACCGG 
GATGATGACT ACGCTGGCTT TGTCTTTGGC TATCAGTCAA GCAGCCGCTT CTATGTGGTG 
ATGTGGAAGC AGGTGACCCA GACCTACTGG GAAGACAAGC CCAGTCGGGC TTACGGCTAC 
TCTGGTGTGT CACTCAAAGT GGTAAACTCC ACGACTGGTA CTGGCGAGCA CCTGAGGAAT 
GCCCTGTGGC ACACGGGAAA CACAGAAGGC CAGGTCCGGA CTCTATGGCA TGACCCCAAA 
AACATTGGCT GGAAAGACTA CACTGCCTAC AGGTGGCACC TGATTCACAG GCCTAAGACA 
GGCTACATGA GAGTCTTAGT GCATGAAGGA AAGCAAGTCA TGGCTGACTC AGGACCAATT 
TATGACCAAA CCTACGCTGG TGGACGGCTG GGCCTGTTTG TCTTCTCCCA AGAGATGGTC 
TATTTCTCGG ACCTCAAGTA TGAGTGCAGA GATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035711.2
CDS	391..3909
Translation	MLWALALLALGIGPRASAGDHVKDTSFDLFSISNINRKTIGAKQFRGPDPGVPAYRFVRFDYIPPVNTDDLNRIVKLARRKEGFFLTAQLKQDRKSRGTLLVLEGPGTSQRQFEIVSNGPGDTLDLNYWVEGNQHTNFLEDVGLADSQWKNVTVQVASDTYSLYVGCDLIDSVTLEEPFYEQLEVDRSRMYVAKGASRESHFRGLLQNVHLVFADSVEDILSKKGCQHSQGAEVNTISEHTETLHLSPHITTDLVVQGVEKAQEVCTHSCEELSNMMNELSGLHVMVNQLSKNLERVSSDNQFLLELIGGPLKTRNMSACVQEGRIFAENETWVVDSCTTCTCKKFKTVCHQITCSPATCANPSFVEGECCPSCSHSADSDEGWSPWAEWTECSVTCGSGTQQRGRSCDVTSNTCLGPSIQTRTCSLGKCDTRIRQNGGWSHWSPWSSCSVTCGVGNVTRIRLCNSPVPQMGGKNCKGSGRETKPCQRDPCPIDGRWSPWSPWSACTVTCAGGIRERSRVCNSPEPQYGGKDCVGDVTEHQMCNKRSCPIDGCLSNPCFPGAKCNSFPDGSWSCGSCPVGFLGNGTHCEDLDECAVVTDICFSTNKAPRCVNTNPGFHCLPCPPRYKGNQPFGVGLEDARTEKQVCEPENPCKDKTHSCHKNAECIYLGHFSDPMYKCECQIGYAGDGLICGEDSDLDGWPNNNLVCATNATYHCIKDNCPKLPNSGQEDFDKDGIGDACDEDDDNDGVSDEKDNCQLLFNPRQLDYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDACSVDIDGDDVFNERDNCPYVYNTDQRDTDGDGVGDHCDNCPLMHNPDQIDQDNDLVGDQCDNNEDIDDDGHQNNQDNCPYISNSNQADHDNDGKGDACDSDDDNDGVPDDRDNCRLVFNPDQEDSDGDGRGDICKDDFDNDNVPDIDDVCPENNAITETDFRNFQMVPLDPKGTTQIDPNWVIRHQGKELVQTANSDPGIAVGFDEFGSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVVMWKQVTQTYWEDKPSRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTEGQVRTLWHDPKNIGWKDYTAYRWHLIHRPKTGYMRVLVHEGKQVMADSGPIYDQTYAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	NM_011581.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin 2 (Thbs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011581.3

ORF Insert Sequence:

ATGCTCTGGG CACTGGCCCT GCTGGCTCTG GGCATAGGGC CAAGAGCTTC TGCTGGTGAC 
CACGTCAAGG ACACTTCATT TGACCTTTTC AGCATCAGCA ACATTAACCG GAAGACCATC 
GGTGCCAAGC AGTTCCGAGG GCCTGACCCC GGGGTGCCCG CCTACCGTTT TGTACGGTTT 
GACTACATCC CCCCAGTGAA CACAGATGAT CTCAACAGGA TTGTCAAGCT TGCAAGGAGA 
AAGGAGGGCT TCTTCCTCAC AGCCCAACTG AAGCAGGACC GCAAGTCTCG GGGAACGCTC 
CTGGTGTTGG AAGGCCCCGG CACCTCCCAG AGGCAGTTTG AGATTGTGTC CAATGGCCCA 
GGGGACACTT TGGACCTCAA CTACTGGGTA GAAGGCAATC AGCATACCAA CTTCCTGGAG 
GATGTGGGCC TGGCTGACTC CCAGTGGAAG AATGTGACTG TGCAGGTGGC CAGTGACACC 
TATAGCCTGT ATGTGGGCTG CGATCTTATC GACAGTGTCA CCCTGGAAGA ACCATTCTAT 
GAGCAGCTAG AAGTAGACAG GAGCAGGATG TACGTGGCCA AAGGTGCATC TCGAGAGAGT 
CACTTCAGGG GCTTGCTGCA GAATGTCCAT CTCGTGTTTG CAGATTCTGT GGAAGATATC 
TTAAGCAAGA AAGGCTGTCA ACACAGCCAG GGAGCTGAAG TCAACACCAT CAGTGAACAT 
ACAGAGACTC TCCATCTGAG CCCTCACATC ACCACAGATC TCGTGGTCCA GGGTGTGGAG 
AAGGCACAGG AGGTGTGTAC GCACTCCTGC GAGGAGTTGA GCAACATGAT GAATGAGCTC 
TCTGGACTGC ACGTCATGGT GAACCAGCTG AGCAAGAACC TGGAGAGAGT GTCTAGTGAT 
AACCAGTTCC TTTTGGAGCT CATTGGGGGC CCTCTGAAGA CAAGAAACAT GTCAGCCTGT 
GTGCAGGAGG GCCGAATCTT TGCAGAAAAT GAAACCTGGG TTGTGGATAG TTGTACCACA 
TGCACCTGCA AGAAATTTAA AACAGTCTGC CATCAGATCA CCTGCTCACC TGCAACTTGT 
GCCAACCCAT CTTTTGTGGA AGGCGAGTGC TGTCCATCCT GTTCACACTC TGCAGACAGT 
GATGAGGGCT GGTCTCCGTG GGCAGAGTGG ACCGAGTGTT CTGTCACCTG TGGCTCTGGG 
ACCCAGCAGA GAGGCCGGTC TTGTGATGTC ACCAGCAACA CCTGCCTGGG CCCCTCCATT 
CAGACAAGGA CATGCAGCCT GGGCAAATGT GATACGAGAA TCCGTCAGAA TGGAGGCTGG 
AGTCACTGGT CACCCTGGTC TTCATGCTCC GTGACTTGTG GAGTTGGCAA TGTCACCCGC 
ATACGTCTCT GCAACTCACC AGTGCCCCAG ATGGGTGGCA AGAACTGCAA GGGCAGCGGC 
CGGGAAACCA AACCCTGTCA GCGTGATCCG TGCCCAATTG ATGGCCGCTG GAGCCCCTGG 
TCCCCTTGGT CAGCCTGCAC AGTTACCTGT GCTGGAGGGA TCCGTGAGCG CTCACGTGTT 
TGCAACAGCC CTGAGCCCCA GTATGGAGGG AAGGACTGTG TCGGGGATGT GACAGAACAC 
CAAATGTGCA ACAAGAGAAG CTGCCCTATT GATGGGTGCT TATCCAACCC GTGTTTTCCT 
GGAGCCAAGT GCAACAGCTT CCCTGATGGG TCCTGGTCCT GTGGCTCCTG CCCAGTGGGC 
TTTCTGGGCA ATGGTACCCA CTGTGAGGAC CTGGATGAGT GTGCTGTGGT CACAGATATT 
TGCTTCTCAA CTAACAAAGC TCCCCGCTGT GTCAACACCA ACCCGGGCTT CCACTGCCTG 
CCTTGTCCAC CACGCTACAA GGGGAACCAA CCCTTCGGTG TTGGCCTGGA GGATGCTAGG 
ACAGAAAAAC AAGTGTGTGA GCCAGAGAAT CCATGTAAGG ACAAGACTCA CAGCTGCCAC 
AAGAATGCAG AGTGCATCTA CCTGGGCCAC TTTAGTGACC CCATGTACAA GTGTGAGTGC 
CAGATTGGCT ACGCAGGTGA TGGGCTCATC TGCGGGGAGG ACTCAGACCT GGATGGCTGG 
CCCAACAACA ACCTGGTGTG TGCTACTAAT GCCACCTACC ACTGCATCAA GGACAACTGC 
CCCAAACTGC CAAATTCCGG GCAGGAGGAT TTTGATAAGG ATGGAATCGG AGATGCTTGT 
GACGAGGACG ATGACAATGA CGGTGTGAGC GATGAGAAGG ACAATTGCCA GCTTCTCTTC 
AATCCCCGTC AATTAGACTA TGACAAGGAT GAGGTTGGAG ACCGCTGTGA CAACTGCCCC 
TATGTGCACA ACCCAGCACA GATCGACACA GACAACAATG GCGAGGGGGA TGCCTGCTCT 
GTGGACATTG ACGGAGACGA TGTTTTCAAT GAGCGAGACA ATTGTCCATA TGTCTACAAC 
ACTGACCAGA GAGATACGGA TGGTGATGGC GTGGGTGACC ACTGTGACAA TTGTCCTCTG 
ATGCACAACC CAGATCAGAT CGATCAGGAC AATGATCTCG TTGGAGACCA GTGTGACAAC 
AATGAGGACA TAGATGATGA CGGCCACCAG AACAACCAAG ACAACTGCCC ATACATCTCC 
AACTCCAACC AGGCTGACCA TGACAACGAC GGCAAGGGCG ATGCCTGCGA CTCTGATGAT 
GACAATGATG GTGTTCCAGA TGACAGGGAC AACTGTCGGC TTGTGTTCAA CCCAGACCAG 
GAAGACTCGG ACGGTGACGG CCGAGGTGAC ATTTGTAAAG ATGACTTTGA CAATGATAAT 
GTCCCAGATA TTGATGATGT GTGCCCTGAG AACAATGCCA TCACTGAGAC AGACTTCAGA 
AACTTCCAGA TGGTCCCTCT GGATCCCAAG GGGACCACAC AAATTGATCC CAACTGGGTA 
ATTCGTCACC AAGGCAAAGA GCTGGTGCAG ACAGCAAACT CAGACCCTGG CATCGCTGTA 
GGTTTCGACG AGTTTGGGTC TGTGGACTTC AGTGGCACTT TCTATGTCAA CACTGACCGG 
GATGATGACT ACGCTGGCTT TGTCTTTGGC TATCAGTCAA GCAGCCGCTT CTATGTGGTG 
ATGTGGAAGC AGGTGACCCA GACCTACTGG GAAGACAAGC CCAGTCGGGC TTACGGCTAC 
TCTGGTGTGT CACTCAAAGT GGTAAACTCC ACGACTGGTA CTGGCGAGCA CCTGAGGAAT 
GCCCTGTGGC ACACGGGAAA CACAGAAGGC CAGGTCCGGA CTCTATGGCA TGACCCCAAA 
AACATTGGCT GGAAAGACTA CACTGCCTAC AGGTGGCACC TGATTCACAG GCCTAAGACA 
GGCTACATGA GAGTCTTAGT GCATGAAGGA AAGCAAGTCA TGGCTGACTC AGGACCAATT 
TATGACCAAA CCTACGCTGG TGGACGGCTG GGCCTGTTTG TCTTCTCCCA AGAGATGGTC 
TATTTCTCGG ACCTCAAGTA TGAGTGCAGA GATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular codes and in vitro generation of hypocretin and melanin concentrating hormone neurons.
Proceedings of the National Academy of Sciences of the United States of America116(34)17061-17070(2019 Aug)
Seifinejad A,Li S,Mikhail C,Vassalli A,Pradervand S,Arribat Y,Pezeshgi Modarres H,Allen B,John RM,Amati F,Tafti M

Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability.
Nature communications10(1)76(2019 01)
Schips TG,Vanhoutte D,Vo A,Correll RN,Brody MJ,Khalil H,Karch J,Tjondrokoesoemo A,Sargent MA,Maillet M,Ross RS,Molkentin JD

Myh10 deficiency leads to defective extracellular matrix remodeling and pulmonary disease.
Nature communications9(1)4600(2018 11)
Kim HT,Yin W,Jin YJ,Panza P,Gunawan F,Grohmann B,Buettner C,Sokol AM,Preussner J,Guenther S,Kostin S,Ruppert C,Bhagwat AM,Ma X,Graumann J,Looso M,Guenther A,Adelstein RS,Offermanns S,Stainier DYR

Sexually dimorphic behavior, neuronal activity, and gene expression in Chd8-mutant mice.
Nature neuroscience21(9)1218-1228(2018 09)
Jung H,Park H,Choi Y,Kang H,Lee E,Kweon H,Roh JD,Ellegood J,Choi W,Kang J,Rhim I,Choi SY,Bae M,Kim SG,Lee J,Chung C,Yoo T,Park H,Kim Y,Ha S,Um SM,Mo S,Kwon Y,Mah W,Bae YC,Kim H,Lerch JP,Paik SB,Kim E

MicroRNA-221-3p, a TWIST2 target, promotes cervical cancer metastasis by directly targeting THBS2.
Cell death & disease8(12)3220(2017 12)
Wei WF,Zhou CF,Wu XG,He LN,Wu LF,Chen XJ,Yan RM,Zhong M,Yu YH,Liang L,Wang W

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Thbs2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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