Vps16B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps16B
Entrez Gene ID	43521
Full Name	Vacuolar protein sorting 16B
Synonyms	CG18112,Dmel\CG18112,Vps16b,dVps16B,fob
General protein information	Preferred Names Vacuolar protein sorting 16B Names CG18112 gene product from transcript CG18112-RA CG18112-PA Vps16B-PA full-of-bacteria vacuolar protein sorting 16B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99C2-99C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_143474.3	NP_651731.1	vacuolar protein sorting 16B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VIPAS39	XP_001164738.1
Mus musculus (house mouse)	Vipas39	NP_001136053.1
Xenopus tropicalis (tropical clawed frog)	vipas39	NP_001120552.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001233	XP_321923.5
Homo sapiens (human)	VIPAS39	NP_001180243.1
Macaca mulatta (Rhesus monkey)	VIPAS39	XP_001102980.1
Gallus gallus (chicken)	VIPAR	XP_004941895.1
Canis lupus familiaris (dog)	VIPAS39	XP_005623764.1
Bos taurus (cattle)	VIPAS39	NP_001091469.1
Rattus norvegicus (Norway rat)	Vipas39	NP_001094474.1
Drosophila melanogaster (fruit fly)	Vps16B	NP_651731.1
Danio rerio (zebrafish)	vipas39	NP_001001836.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps16B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps16B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06548	NM_143474.3 Latest version!	Drosophila melanogaster vacuolar protein sorting 16B (Vps16B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps16B ( NM_143474.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651731.1 Drosophila melanogaster vacuolar protein sorting 16B (Vps16B), mRNA.

Price & Availability↑

CloneID	OFa06548
Clone ID Related Accession (Same CDS sequence)	NM_143474.3
Accession Version	NM_143474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 16B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143474.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCTGC AATTGGACAG CGAAAGCTAT TGGAACCGAT CCTCTCGCGC AGCGTTCAGC 
TTCGACGACG AAGACGAGGT GGACCTCGCC CCGGATCTGG TCAGCAATGG CATCCTCGCC 
GACGACACCA TTTCGGAGGC CAGCTTCAAT AACAGTGTCG CCCTGAATCT CTCGATCAAA 
TCCATTCTGA GCGAGGAGGC ATTGAAACTG GTGCTGCAGG AACAGGCGCT GGACGACAGG 
GTCCTGCCCA AGGGTGTCTC GCCGGAGGAG GAGCTGAAGC TGCTGCGCCG ACAGCTTCAG 
AGCACCCTGT ACAGCCCCAA TCTAGAAGCC ACTGCACAGA AGCTGCTGCA AGGAAAGACT 
GCGCCCTTGG AGATGTTCAA GTCGCTGCAT GAGAAGCAGC AGCTGCTGGA CACCCTGATG 
GCACAAGGTG GTGGGCATGC CGTCATTACA GTGTTGCTCT TCCTCAAGAG AACTCTGAAT 
ACCACGCAGT TTCATGGCAT TCTGAGGGAG AGACCCAAGG CCTTGGAGCA GTATCTGAGT 
TACCTGAAGG AAAGTGGCGA CCTGGCGGGC CATATTGAGC TACTGCAGCT ATTTGGACGC 
CACCAGGAGG CGGCTCTTAA GCAGTTTCAG GCAGCGCTCG CTTCCGGAGA TACGAGCACC 
AGGAAGAAAC ACCTCCAGCT TTTGGTGGAT GCCTATGCCA CAGCGGGAGT GGGTGTAATA 
CCTCTCTACG AGCAGGTTTT TCACGCTGCC CTCAAGATGC AGCAGCTAAT GGAGAAGGAT 
AGCGCGCTCG GCAAGCTGCT TCGGGATCAA CCCACTCCAG TGGAAGTGCT CTATGCTTGC 
TGCCAGGTGA ACAGCAATTG GAAAGAGCAG GATATGCTCA AACCAGTGTC ACCTCAGCGT 
TTTGCCGCCG ACCAGCAAAT ATCGCCGGCC CAGTACGAGT GGACGGCGCT CAATGAAAGG 
GCGCAGGCAC AGGCTTATGC GGATCTGGAG TGCATATTTG AGCGGGTGCC CAGTTGGCAT 
CCGCTGAAGA CCAAACAGTT TCACATCAGC TTCGATCTGA CGCTGGCCGT GCTCCGGCTG 
TACGAGCTGC AGGCTCCCTC GACCGTCCTT CAGCTATTCC TCTCCAAGAT GGGCAACAGC 
GTTGAGAAAC TGGCGCTAGC CCAGCGGGTG AAGTGCATCA AGGCGGTCAT AGATGCCATG 
GCCGGGTTGA AGCAGCAGCA GGAGCTACAG CAGCTCAAGG ACACCCTGCC CGAGCGATCC 
GAGGAGCAGT TCTACTGCGA GAATGCGCTG AAAAGCCTCC AGAGCAAGCG ATGGACTACG 
GACAACATCA AGCTCAAGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651731.1
CDS	46..1389
Translation	MDLQLDSESYWNRSSRAAFSFDDEDEVDLAPDLVSNGILADDTISEASFNNSVALNLSIKSILSEEALKLVLQEQALDDRVLPKGVSPEEELKLLRRQLQSTLYSPNLEATAQKLLQGKTAPLEMFKSLHEKQQLLDTLMAQGGGHAVITVLLFLKRTLNTTQFHGILRERPKALEQYLSYLKESGDLAGHIELLQLFGRHQEAALKQFQAALASGDTSTRKKHLQLLVDAYATAGVGVIPLYEQVFHAALKMQQLMEKDSALGKLLRDQPTPVEVLYACCQVNSNWKEQDMLKPVSPQRFAADQQISPAQYEWTALNERAQAQAYADLECIFERVPSWHPLKTKQFHISFDLTLAVLRLYELQAPSTVLQLFLSKMGNSVEKLALAQRVKCIKAVIDAMAGLKQQQELQQLKDTLPERSEEQFYCENALKSLQSKRWTTDNIKLKL

Target ORF information:

RefSeq Version	NM_143474.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 16B (Vps16B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143474.3

ORF Insert Sequence:

ATGGACCTGC AATTGGACAG CGAAAGCTAT TGGAACCGAT CCTCTCGCGC AGCGTTCAGC 
TTCGACGACG AAGACGAGGT GGACCTCGCC CCGGATCTGG TCAGCAATGG CATCCTCGCC 
GACGACACCA TTTCGGAGGC CAGCTTCAAT AACAGTGTCG CCCTGAATCT CTCGATCAAA 
TCCATTCTGA GCGAGGAGGC ATTGAAACTG GTGCTGCAGG AACAGGCGCT GGACGACAGG 
GTCCTGCCCA AGGGTGTCTC GCCGGAGGAG GAGCTGAAGC TGCTGCGCCG ACAGCTTCAG 
AGCACCCTGT ACAGCCCCAA TCTAGAAGCC ACTGCACAGA AGCTGCTGCA AGGAAAGACT 
GCGCCCTTGG AGATGTTCAA GTCGCTGCAT GAGAAGCAGC AGCTGCTGGA CACCCTGATG 
GCACAAGGTG GTGGGCATGC CGTCATTACA GTGTTGCTCT TCCTCAAGAG AACTCTGAAT 
ACCACGCAGT TTCATGGCAT TCTGAGGGAG AGACCCAAGG CCTTGGAGCA GTATCTGAGT 
TACCTGAAGG AAAGTGGCGA CCTGGCGGGC CATATTGAGC TACTGCAGCT ATTTGGACGC 
CACCAGGAGG CGGCTCTTAA GCAGTTTCAG GCAGCGCTCG CTTCCGGAGA TACGAGCACC 
AGGAAGAAAC ACCTCCAGCT TTTGGTGGAT GCCTATGCCA CAGCGGGAGT GGGTGTAATA 
CCTCTCTACG AGCAGGTTTT TCACGCTGCC CTCAAGATGC AGCAGCTAAT GGAGAAGGAT 
AGCGCGCTCG GCAAGCTGCT TCGGGATCAA CCCACTCCAG TGGAAGTGCT CTATGCTTGC 
TGCCAGGTGA ACAGCAATTG GAAAGAGCAG GATATGCTCA AACCAGTGTC ACCTCAGCGT 
TTTGCCGCCG ACCAGCAAAT ATCGCCGGCC CAGTACGAGT GGACGGCGCT CAATGAAAGG 
GCGCAGGCAC AGGCTTATGC GGATCTGGAG TGCATATTTG AGCGGGTGCC CAGTTGGCAT 
CCGCTGAAGA CCAAACAGTT TCACATCAGC TTCGATCTGA CGCTGGCCGT GCTCCGGCTG 
TACGAGCTGC AGGCTCCCTC GACCGTCCTT CAGCTATTCC TCTCCAAGAT GGGCAACAGC 
GTTGAGAAAC TGGCGCTAGC CCAGCGGGTG AAGTGCATCA AGGCGGTCAT AGATGCCATG 
GCCGGGTTGA AGCAGCAGCA GGAGCTACAG CAGCTCAAGG ACACCCTGCC CGAGCGATCC 
GAGGAGCAGT TCTACTGCGA GAATGCGCTG AAAAGCCTCC AGAGCAAGCG ATGGACTACG 
GACAACATCA AGCTCAAGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps16B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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