Cul5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cul5
Entrez Gene ID	43434
Full Name	Cullin 5
Synonyms	CG1401,CUL5,Cul-5,Cullin5,Dmel\CG1401,cul-5,cul5
General protein information	Preferred Names Cullin 5 Names CG1401 gene product from transcript CG1401-RA CG1401-PA Cul5-PA Cullin-5 cullin cullin 5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F6-98F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_143408.3	NP_651665.2	cullin 5

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cul5	NP_082083.2
Canis lupus familiaris (dog)	CUL5	XP_005619886.1
Rattus norvegicus (Norway rat)	Cul5	NP_073174.1
Drosophila melanogaster (fruit fly)	Cul-5	NP_651665.2
Xenopus tropicalis (tropical clawed frog)	cul5	NP_001072836.1
Homo sapiens (human)	CUL5	NP_003469.2
Macaca mulatta (Rhesus monkey)	CUL5	XP_001103587.2
Caenorhabditis elegans (roundworm)	cul-5	NP_505616.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002404	XP_312548.5
Danio rerio (zebrafish)	cul5b	XP_005161301.1
Pan troglodytes (chimpanzee)	CUL5	XP_522173.3
Bos taurus (cattle)	CUL5	XP_002693012.1
Gallus gallus (chicken)	CUL5	XP_417163.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cul5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cul5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06454	NM_143408.3 Latest version!	Drosophila melanogaster cullin 5 (Cul5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cul5 ( NM_143408.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651665.2 Drosophila melanogaster cullin 5 (Cul5), mRNA.

Price & Availability↑

CloneID	OFa06454
Clone ID Related Accession (Same CDS sequence)	NM_143408.3
Accession Version	NM_143408.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	cullin 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143408.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTACGGG CAATCGGGAA ACGGGACAGG GACATCTTCG AGGAAGTTTG GCCGGACAAA 
AGGCGAATAG TTTTAAAGCT ACTGCGCCAG GACACGGTGT CGCAGCGCGA ATGGCAGGAC 
CTATTCTTCG GCGTACACTT CGTGTGCCTG TGGGACGAGA AGGGCGCGGC CAAGATCTAC 
GACTGCCTGC AGCAGGACAT CGTTGAGTTT ATCGTCCAGG CCCAAAGCCA GGTGCAAGCG 
CAACGGGGCG AGCAGGCCCT TCTGGCCACC TATATTGTGG AGTGGCGGAA GTTCTTTACA 
CAGTCCAACT ACCTGCCGCT GCCTTTCCGC CAGCTGGAGC AGTCGCCCCA GGTGAAGCCC 
TCCTCCAGTG GCAGCAGCTC CTCCGCATCC GTCTCCGCCT CGGGAGCGAG TACGAGTGCC 
GCCGCGGCGG CAGCAGCTAC TTCCTGCAGC GGCCAGGCTG GAAAGTCAGG AGCCTCCACC 
TCAGCCGCAG CTGGAGCCTC CACTTCAGCC GCCGCTGCTG CAGCCTCCAC ATCCGCCTCC 
ACAAGCTCAG CAGCGGCAGC TGCAGCTGCT TCCGGAAGTG GGAGCTCCAG CAGCAGCACC 
TCTACCTCCA AGAAAAACCC CACGGAGGAC AGTCCTGTAC GAAAACTAAT GCTGGACTCC 
TGGAACAAGC ACATATTCCA CGACATCAAG CATCGTCTGC AGGAGTCGGC CATGAAGATT 
GTGCACGCCG AGCGGAACGG CGATGCCTAT GATGCCCAGC TAGTGGTAGG CGTGCGCGAG 
AGCTATGTGA ATCTCTCCTC GAATGCAGAG GACAAACTAG AGATCTACAG GGAGAACTTT 
GAGATGGCGT ACCTTAAGGC CACTGTCGAG TTCTATCGCC TGAAATCCGC CGAACAACAG 
CAAGAGAACG GAGTGCTGGC CTATATGAAA TACGCCGACT CAAAGTTGCG TGAGGAGGAG 
GTTAGGGCCA AGCGGTATCT GGAGCCCAGC AGCTTCAGTA TACTTACTTA CACGCTGGTT 
AATGTGCTCA TAGTGGACCA CCTCAACTCA ATCATCGCCG AGTGTCCGGC TTTGATCAGG 
GACTATGAAA CGGAGCGATT GAATCTCATG TTCCGCCTGA TGGATAGAGT GATGCACGGC 
GTTGGCGTGG AGCCCATGAT GGGTGACCTG CAGCGTCACA TCATGTCCGC CGGACTGGCC 
GACATGCTGT CCGCTTCGGA GGTGATTACC CAGGATTCGG AAAAGTACGT TGAGCGGCTA 
CTTGAGCTGT TCAACAAGTT CAGCGACCTG GTACGCAACG CCTTCAACGA CGATCCCCGC 
TTCCTCACTG CCCGCGACAT TGCTTTTAAG ACGGTAGTCA ACGACACCAG CGTGTTCAAG 
ATGGAGCTAC CCACGAGTAT CGCCAATCGG GGTGTAAAGT ACACAGCGCC GGAGAGCAAA 
TGCCCGGAAC TACTGGCCAA CTATTGCGAC ATGCTGCTGC GGCGAACGCC GCTCAGCAAA 
CGCCTCACCA GCGAACAGAT CGACGCCCGT CTGCGCGATG TCCTCTTGGT CCTGAAGTAC 
GTCAACAACA AGGACGTCTT TATGCGCTAT CACAAAGTTC ATTTGACGAG GCGCTTGATT 
CTGGGGACCA GTGCGGACAG CGAAAAGGAG GAGGACATAG TCGAGTGGCT GAGGGAGGTG 
GGCATGCCGG CGGACTACGT GAATCGGCTG GCGCGCATGT TCCAAGACAT AAAGGTCAGC 
GAAGACTTAA ATACTCAATT CCGCACCTCG ATAAGTCGGC ACGATGCTAT CAACATAAAA 
ATACTCAACG CGGGTGCCTG GGCCCGCTGT TCGGAGCGAG TCTCGGTCTC ACTGCCCATC 
GAGCTCGAGG ACTATATTCC TGATGTGGAG GAGTTTTACA AGAAGAAGCA TTCCGGCCGT 
AAGCTGCAGT GGTACCATCA CATGAGCAAT GGCACTATCA CCTTTGTGAA TAACTTCGGG 
CGCTACGATC TGGATGTGAC CACTTTCCAG ATGGCCGTCT TGTTTGCCTG GAATCAGCGT 
CAGCATGACA AAATTTCCTA CGAGAATCTC CGCCTGGCCA CAGAACTACC AGATCCCGAA 
CTGAGGCGAA CGTTGTGGTC GCTGGTAGCG TTCCCTAAGA TAAAGAAACA GATCCTGCTC 
ATGGAACCGG CGGCGATCAG TTCGCCCAAG GACTTTGCCG AGAACACAAT GTTCTATATT 
AATCAGGAAT TTGCTATTGT CAAGAATGGC AAATCCCAGC GTCGTGGAAA GCTGAATCTC 
ATCGGGCGAC TGCAATTGAG CACGGAGCGC TCGCAGCAGG AGGACAATCA ATCCATTGTG 
CAACTGCGTA TTCTGCGCAC ACAAGAGGCC ATTATCAAAA TCATGAAAGT GCGCAAGCGG 
ATGAACAACG CAGCGCTGCA GGGTGAACTC ATCGACATAT TGAAGAACAT GTTCCTGCCG 
TCCAAGAAGA TGATCAAGGA GCAGCTGGAG TGGCTGATTG AGAACAAGTA TATGAGACGG 
GACGACGATG ATATCAACAT GTTCATTTAC GTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651665.2
CDS	86..2644
Translation	MLRAIGKRDRDIFEEVWPDKRRIVLKLLRQDTVSQREWQDLFFGVHFVCLWDEKGAAKIYDCLQQDIVEFIVQAQSQVQAQRGEQALLATYIVEWRKFFTQSNYLPLPFRQLEQSPQVKPSSSGSSSSASVSASGASTSAAAAAAATSCSGQAGKSGASTSAAAGASTSAAAAAASTSASTSSAAAAAAASGSGSSSSSTSTSKKNPTEDSPVRKLMLDSWNKHIFHDIKHRLQESAMKIVHAERNGDAYDAQLVVGVRESYVNLSSNAEDKLEIYRENFEMAYLKATVEFYRLKSAEQQQENGVLAYMKYADSKLREEEVRAKRYLEPSSFSILTYTLVNVLIVDHLNSIIAECPALIRDYETERLNLMFRLMDRVMHGVGVEPMMGDLQRHIMSAGLADMLSASEVITQDSEKYVERLLELFNKFSDLVRNAFNDDPRFLTARDIAFKTVVNDTSVFKMELPTSIANRGVKYTAPESKCPELLANYCDMLLRRTPLSKRLTSEQIDARLRDVLLVLKYVNNKDVFMRYHKVHLTRRLILGTSADSEKEEDIVEWLREVGMPADYVNRLARMFQDIKVSEDLNTQFRTSISRHDAINIKILNAGAWARCSERVSVSLPIELEDYIPDVEEFYKKKHSGRKLQWYHHMSNGTITFVNNFGRYDLDVTTFQMAVLFAWNQRQHDKISYENLRLATELPDPELRRTLWSLVAFPKIKKQILLMEPAAISSPKDFAENTMFYINQEFAIVKNGKSQRRGKLNLIGRLQLSTERSQQEDNQSIVQLRILRTQEAIIKIMKVRKRMNNAALQGELIDILKNMFLPSKKMIKEQLEWLIENKYMRRDDDDINMFIYVA

Target ORF information:

RefSeq Version	NM_143408.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cullin 5 (Cul5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143408.3

ORF Insert Sequence:

ATGCTACGGG CAATCGGGAA ACGGGACAGG GACATCTTCG AGGAAGTTTG GCCGGACAAA 
AGGCGAATAG TTTTAAAGCT ACTGCGCCAG GACACGGTGT CGCAGCGCGA ATGGCAGGAC 
CTATTCTTCG GCGTACACTT CGTGTGCCTG TGGGACGAGA AGGGCGCGGC CAAGATCTAC 
GACTGCCTGC AGCAGGACAT CGTTGAGTTT ATCGTCCAGG CCCAAAGCCA GGTGCAAGCG 
CAACGGGGCG AGCAGGCCCT TCTGGCCACC TATATTGTGG AGTGGCGGAA GTTCTTTACA 
CAGTCCAACT ACCTGCCGCT GCCTTTCCGC CAGCTGGAGC AGTCGCCCCA GGTGAAGCCC 
TCCTCCAGTG GCAGCAGCTC CTCCGCATCC GTCTCCGCCT CGGGAGCGAG TACGAGTGCC 
GCCGCGGCGG CAGCAGCTAC TTCCTGCAGC GGCCAGGCTG GAAAGTCAGG AGCCTCCACC 
TCAGCCGCAG CTGGAGCCTC CACTTCAGCC GCCGCTGCTG CAGCCTCCAC ATCCGCCTCC 
ACAAGCTCAG CAGCGGCAGC TGCAGCTGCT TCCGGAAGTG GGAGCTCCAG CAGCAGCACC 
TCTACCTCCA AGAAAAACCC CACGGAGGAC AGTCCTGTAC GAAAACTAAT GCTGGACTCC 
TGGAACAAGC ACATATTCCA CGACATCAAG CATCGTCTGC AGGAGTCGGC CATGAAGATT 
GTGCACGCCG AGCGGAACGG CGATGCCTAT GATGCCCAGC TAGTGGTAGG CGTGCGCGAG 
AGCTATGTGA ATCTCTCCTC GAATGCAGAG GACAAACTAG AGATCTACAG GGAGAACTTT 
GAGATGGCGT ACCTTAAGGC CACTGTCGAG TTCTATCGCC TGAAATCCGC CGAACAACAG 
CAAGAGAACG GAGTGCTGGC CTATATGAAA TACGCCGACT CAAAGTTGCG TGAGGAGGAG 
GTTAGGGCCA AGCGGTATCT GGAGCCCAGC AGCTTCAGTA TACTTACTTA CACGCTGGTT 
AATGTGCTCA TAGTGGACCA CCTCAACTCA ATCATCGCCG AGTGTCCGGC TTTGATCAGG 
GACTATGAAA CGGAGCGATT GAATCTCATG TTCCGCCTGA TGGATAGAGT GATGCACGGC 
GTTGGCGTGG AGCCCATGAT GGGTGACCTG CAGCGTCACA TCATGTCCGC CGGACTGGCC 
GACATGCTGT CCGCTTCGGA GGTGATTACC CAGGATTCGG AAAAGTACGT TGAGCGGCTA 
CTTGAGCTGT TCAACAAGTT CAGCGACCTG GTACGCAACG CCTTCAACGA CGATCCCCGC 
TTCCTCACTG CCCGCGACAT TGCTTTTAAG ACGGTAGTCA ACGACACCAG CGTGTTCAAG 
ATGGAGCTAC CCACGAGTAT CGCCAATCGG GGTGTAAAGT ACACAGCGCC GGAGAGCAAA 
TGCCCGGAAC TACTGGCCAA CTATTGCGAC ATGCTGCTGC GGCGAACGCC GCTCAGCAAA 
CGCCTCACCA GCGAACAGAT CGACGCCCGT CTGCGCGATG TCCTCTTGGT CCTGAAGTAC 
GTCAACAACA AGGACGTCTT TATGCGCTAT CACAAAGTTC ATTTGACGAG GCGCTTGATT 
CTGGGGACCA GTGCGGACAG CGAAAAGGAG GAGGACATAG TCGAGTGGCT GAGGGAGGTG 
GGCATGCCGG CGGACTACGT GAATCGGCTG GCGCGCATGT TCCAAGACAT AAAGGTCAGC 
GAAGACTTAA ATACTCAATT CCGCACCTCG ATAAGTCGGC ACGATGCTAT CAACATAAAA 
ATACTCAACG CGGGTGCCTG GGCCCGCTGT TCGGAGCGAG TCTCGGTCTC ACTGCCCATC 
GAGCTCGAGG ACTATATTCC TGATGTGGAG GAGTTTTACA AGAAGAAGCA TTCCGGCCGT 
AAGCTGCAGT GGTACCATCA CATGAGCAAT GGCACTATCA CCTTTGTGAA TAACTTCGGG 
CGCTACGATC TGGATGTGAC CACTTTCCAG ATGGCCGTCT TGTTTGCCTG GAATCAGCGT 
CAGCATGACA AAATTTCCTA CGAGAATCTC CGCCTGGCCA CAGAACTACC AGATCCCGAA 
CTGAGGCGAA CGTTGTGGTC GCTGGTAGCG TTCCCTAAGA TAAAGAAACA GATCCTGCTC 
ATGGAACCGG CGGCGATCAG TTCGCCCAAG GACTTTGCCG AGAACACAAT GTTCTATATT 
AATCAGGAAT TTGCTATTGT CAAGAATGGC AAATCCCAGC GTCGTGGAAA GCTGAATCTC 
ATCGGGCGAC TGCAATTGAG CACGGAGCGC TCGCAGCAGG AGGACAATCA ATCCATTGTG 
CAACTGCGTA TTCTGCGCAC ACAAGAGGCC ATTATCAAAA TCATGAAAGT GCGCAAGCGG 
ATGAACAACG CAGCGCTGCA GGGTGAACTC ATCGACATAT TGAAGAACAT GTTCCTGCCG 
TCCAAGAAGA TGATCAAGGA GCAGCTGGAG TGGCTGATTG AGAACAAGTA TATGAGACGG 
GACGACGATG ATATCAACAT GTTCATTTAC GTGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cul5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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