mbc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mbc
Entrez Gene ID	42817
Full Name	myoblast city
Synonyms	CG10379,DOCK180,Dm MBC,Dmel\CG10379,Dock180,MBC,MBC/DOCK180,Mbc,Su(rac)1,dock180
General protein information	Preferred Names myoblast city Names CG10379 gene product from transcript CG10379-RB CG10379-PA CG10379-PB mbc-PA mbc-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95B5-95B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260338.2	NP_001247267.1	myoblast city, isoform B
NM_057796.4	NP_477144.1	myoblast city, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dock1	NP_001028592.1
Danio rerio (zebrafish)	dock1	NP_001103481.1
Bos taurus (cattle)	DOCK1	XP_005225929.1
Rattus norvegicus (Norway rat)	Dock1	NP_001137330.1
Caenorhabditis elegans (roundworm)	ced-5	NP_501938.1
Homo sapiens (human)	DOCK1	NP_001371.1
Canis lupus familiaris (dog)	DOCK1	XP_544064.4
Drosophila melanogaster (fruit fly)	mbc	NP_001247267.1
Xenopus tropicalis (tropical clawed frog)	dock1	XP_002932708.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004320	XP_313591.5
Gallus gallus (chicken)	DOCK1	XP_421819.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mbc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mbc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05840	NM_057796.4 Latest version!	Drosophila melanogaster myoblast city (mbc), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa08001	NM_001260338.2 Latest version!	Drosophila melanogaster myoblast city (mbc), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mbc ( NM_057796.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477144.1 Drosophila melanogaster myoblast city (mbc), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05840
Clone ID Related Accession (Same CDS sequence)	NM_057796.4
Accession Version	NM_057796.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	myoblast city, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057796.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTGT GGAGCGACTG CAATGCCAAG CAGGCAGAAT TCGGCATTGC CAAGTGCAAT 
TTCGACCAGG AGTCGAAGCC ACATCGCCTT AATCTGGATG TGGGTGATGC GGTCATAATT 
CTTAAGGAGA CCACCCATTG GTACTACGGC TACAGACAAA AAGCAAAGGA AATACGCGGC 
ATATTTCCCA AGAGCTATAT ACACTTATGC GAATATAATA TCGTGAATGG GGAGTACTGC 
ATCCAGCGCA CGGATATTGT CGAGGAGATC ACTAAAGTTC TTCTCGAGTG GGGCTCCATA 
GCCAAGGATT ACTTTCTGAC CACAAATCCC AGCTTTCCCA AAATTCGACG AAAAATGAAC 
GAATTAAATA ATAATAGGGC GGCTTTGATC TCCGGAAACC TGCCATTGGA CGAGGTGCGA 
AAGGTGAAGC TGCTGGCCAC CAATCAGATC GATACTGGCA ACAAGCTCCT TGGCCTGGAC 
ATGGTGGTTC GCGACGAGAG CGGAGACATC TTGGACACCA ATGCCATCTG CACCACCGAG 
CTCTACGAGC AACATATGCA CGCTGTTCAG CGCATCGACA AGGCCAATCG GCTGTCAAGC 
GAGCGGGGAA CTACAAGAAC ACCTAACAAG TACTCTCACA ACATCCTGCT ACACGTGAAC 
GCCTTCGTTT GCAAGTTCCA GGAGGACTCT GACCTGCTCT TCACGCTTTT CGACGGCGAA 
ACACACAAAC CCATTAGCGA GAACTATGTG GTCAAGTGGT CACGGACAGG TATTGCGCGT 
GATACTGACC AGATCGACAA CAACCGCGTG CTGTTCACGG ATCTCAGTAA GAGCGACCTA 
GCCATTGCCA AAATGTACCT GGTGTGCTAT GCCATCCGAA TCGGTTCCAT GGAGTTTAAG 
GATTCTGCCG AGTCGAAACG GACCAGCATG AGCATTGCCA ACAGCATGCT GAACGCCTCC 
AGTCGCAAGG CCTCCCAGCT ATCGGTGAGC TCCAGTGGAT CGTCCAGCAG CAATGGGGAA 
TACATCATTC GACGTCCGTT TGGTGTGGCC TGCAAGGACC TCACGCCCTT CATCAATAAG 
TCGGACGACT TCCGAGGCAA CATAGACCTG CCCTTCATCA TGTGCGAAAA GGAAACGCTG 
GACGGAACGC TGCGGAAGCT AATCGCGAAT AAGGATATTG GCAAGATTGA CTCCAAGATG 
GCTGTAACTA TCGAGGTGCT TCGGGGCGAC ATCAAGCAGA TAAAGGAAGA ATTTCCACGC 
TTGATGCACA CAAATGTGCC AGTTGCCCGA AAAATGGGAT TTCCTGAGGT CATTTTGCCA 
GGCGATGTGC GAAATGATTT GTACCTTACC ATCTGCAGTG GAGAATTCGC ACGCATTGCC 
AAGACCTCGG AGAAGAACGT TGAGGTGTCG GTGTGTGTGG CCAATGAGCA GGGATACCTT 
ATGCCCGGTG TGCTAAGCAT TGGAGCTGGA CATCAACCCA TTGATGAGTA CAAATCGGTT 
GTATACTACC ACGATGACAA GCCTAAGTGG CAGGAGACCT TCAAGATCCA CGTGCCCATT 
GAAGACTTTA AACAATGTCA TTTGCGCTTT GTGCTCAAAC ATCGTAGCAG TAATGAGCAG 
AAGGATCGAA CTGAAAAACC CTTCGGTCTC GCGTATGTGC GTCTAATGCA GGCCAACGGG 
ACAACCATCA CGCAGGGTCA ACATATCCTG GCCGTTTACA AAATTGATCA CAAAAAGTAC 
GACAAAACAG TAGCCAATTG TTATTTAGAA CTTCCAGCCA CGGTTGCAGA GCTGCAAGGT 
GCAAAGCCTT CTATCGGTGG ACTTACTCTC TTGCCAAAGG ATCAGCTATC GATTGGGGTT 
AACCTGTGCA GCACCAAGCT CACGCAGAGC GTGAGCTTGC TGGGACTTCT GAATTGGTCG 
GCGCACAAGG AGACGCTGGA ACAGTCGCTA AACGCCTTGT CCACAGTGCC TGGAGAGGAG 
GTGGTGAAGT TCCTGCAGGA CATACTCGAT GCCTTGTTTA ATATACTGGT GGAGAATGAT 
CATCCTGAGA AATATGACCA GCTCGTCTTT ATGAGCATTA TACATTTGAT TGAAACGGTG 
TCCGATCTCA AGTACCAACA CTTTCTCAGT GTTCTCGATG TGTACATTAA TGAAAGCTTC 
TCGTTTACAC TTGCCTACAC CAAATTGATG GATGTGCTGC AGAAGAATAT TAGTGAAGCG 
ATCTCACCAA AAGAAAAGTC TGCTGATGGC AATGATCTGG AGGAGAGTCC AGAGGTGCGC 
CGCCTGTACA AGACAACTCG TTACCTTCAC TATGTGATGA AGTTCGTGAT TCGATCGCGT 
GTGCTCTACG CCGAGATGAA CTGCAACACG GACTATATGG ACTTTGCTAC CCGGCTGCAG 
GAGCTTCTTC GCATGTTCAT CGACATGATT GGCTGTCCGA GCAATCTGCT TAAATCGGAG 
GGAGCGCTGC TCAAGAATCT GCACATCATC GCCACAGATT TAATGCAGGT CTTTGAACAT 
GTGCGCTTAA GCATATCCAT TGTGGAAATC CTTGAGAAAT TCCCGCCGCG ACGTCTTACT 
CAGTCTAAGA TGGGATGCAT TAAAGATTTT GTGGAGACAA AACTGTTTAC GTTGCCCAAG 
TGTCGGGCCA TCCTACTGCC CGTGTTCTGC AAACACATCA AGGATCACCT CGAGAGCAAG 
GAGGAGATTG CCGAGTGCAT CAACATCATG AACAACATAC TGAAACTTCT GTTCCGATCG 
GATGTGGGTT CCACGCACAA TGACATTCGA GACATTATGA TCATCCTGTT TCGCACTGTG 
ATGAAGGCAG CTCACGCCCT GGACAGGGAC ACGGGGTTGG TGGGCAAATT CTTTGCCATT 
ATGCTGGGTA TACTGCAGCG CATGGACGCC CAGCACTACG AGTACTTTGT GAAGGACCTG 
CACCAGAGCG GCGAGTTGAA ACACTTTGTC ATAGAGATCC TTCTGGTATT TGAGGAGCTG 
GTGTCTCCGC ACCAGAAAGC AGTGTTTCCG CGGGACTGGA TGGACATGAT TATGCACCAG 
AATACTGTGA TCCTTGGTGC TCTGAAGCAC CTCACCGTGG TCATTACGGA CTACTTCTTG 
TGCCCGTTCG AGAAACAGAT CTGGTCGAAC TTCTTCCAGT GCTCTATTGC CTTTCTGGTG 
CAATCCCCGC TTCAATTGAA TGATTTCAAC GATAATAAAC GGCAAATTGT GTTCGCAAGA 
TATCGTGACA TTCGCAAGGA TACGGCCATG GAGATCAGGA AGATGTGGTT CCAGCTGGGG 
CAGCACAAAC CCAAGTTTGT GCCGCAGTTG GTGGAGCCAA TACTGGAAAT GAGCATGATA 
CCGGAGAAGG AGCTGCGCCA GGAGACCATT CCTATCTTCT TCGACATGAT GCAATGCGAG 
TACTATAGCT CGCGATTGGA ACACGAAAGT TACGGCGACA CCAAATTCAA CAATGCCCAC 
CACAAGGGTA ACTTTTCCGA CTTCAAGACA GCGATGATTG AGAAGTTGGA CATCCTGATT 
GGAGCTGGTA AAGGCGACGC TGAATACAAG CATCTGTTCG AGACAATTAT GCTAGAGCGC 
TGCGCTGCCC ACAACACTCT TAACGTGGAT GGCACTGCCT TTGTGCAGAT GGTAACCCGG 
CTGATGGACA AGTTGTTGGA GTATCGCTTT ATAATCCAGG ATGAGAGCAA GGAGAATCGC 
ATGGCTTGCA CGTTCTCTCT GTTGCAGTTC TATTCGGAGG TGGATCTCAA GGAGATGTAC 
ATACGTTATG TCAACAAACT GTGCGCTCTG CACATGGAGT TCGAGAACTA CACGGAGGCG 
GCGTTCACGC TTAAGCTGCA CACGGAACTG CTGCGTTGGA CGGATACGGA GCTGTCGCAC 
CAACTGCGCA GCTATCGGCA TAATAACTGC CGTACTCACC GGCAACTGAA GGAGGCTCTT 
TACTTCGAGA TCATGGAGTA CTTCGACAAG GGCAAACAGT GGGAGTGCGC CATCGATATG 
TGCAGGGTGC TGGCCCGCCA GTACGAGGAG GAGATCTTCG ACTATCTCAA GCTGGCGGAA 
CTTCTGAATC GCATGGCATT GTTCTATGAG AAGATCATCA AGGAGCTGCG CCACAATTCT 
GAATATTTCC GCGTGTGCTT CTATGGGCGT GGATTCCCGC GGTTTCTTCA GAACCGTGTC 
TACATCTTCC GTGGCAAGGA GTACGAGCGG CACAGTGACT TCTGCGCCCG TATGCTAGTA 
CAGCATCCAC AGGCTGAGCT CATGCAAACG CTGGAGGCTC CGGGCGACGA CATCACCAAC 
AGTGATGGTC AGTACATACA GGTGAACAAG GTGGAGCCGA TAATGGGTCA GGCGTTCAAC 
AAGTTCAACG ATAAGATTAT CAACAACGAA ATCGTGAAAT ACTTCACCGC CAACAACGTG 
CAAAAGTTTC AGTTTTCACG TCCCTTCCGG GACAGCACGA ACGGTGGCGA TAGGGACGAT 
GTACGAAATC TGTGGCTGGA GCGCACAGAG CTGCGTATCA GCTACCCACT TCCGGGTATT 
CTGCGCTGGT TCCCCGTCGT TGAGACCAAT ACTTTTAAAA TCTCTCCCCT AGAGCGGGCG 
GTCGAAATTA TGAAAGATAC GAACCGTGAC ATTCGACAAC TGGTGATCCT GCACAAGAGC 
GACGAAACTC TGCACATTAA TCCGCTGAGT ATGAAACTTA ATGGCATTGT GGATCCGGCT 
GTAATGGGTG GCTTTGCCAA GTACGAGGAA GCCTTCCTAA CCGACGACTA CTTGGAGCAG 
AATCCGGACG ACAAGGAGTT GGTAGAAGAG CTCAAAGAAC TGATTGCGAA CCAGATTCCA 
CTGCTCGATT TAGCAATTCA GCTTCATCGC TTGCGGGCAC CAGATAGTTT GAAAGCGCTG 
CAGGAACACC TGGAGCGTTG CTTCGCCGAC ATGCAGCAGC ATGTGGAGCA GCGCTATGGT 
CGCAAGTCCT GCGACCTCAA AATCGAACGG GATTCCGTGG TGATGCGACG TCCAAACTCC 
TTTCTGCCTT CGCTTTTCGA TGGCAGCAAC AATCGACACT CCGAAACCAG CATGGGATCT 
TCAGACAGCG GCCTGTCAAA GTCAACGTTT CTGCCGCGTC CACAGACGAA TTCTATTAAA 
AACCCATTTT CCGGCCTAAG TTTCAACACA AGGCCCAGTT TGGGACACTC GCCGAGCATT 
AAGAGCAACA AGAGCAAGGA TAAGACTCCG AGTAAGCGAA GAACCAAGGA TGGAAAGGTT 
AAGGAGCGTG AAGCCCATAG CCTGTCCAGC AGTCAGTGGT ACACACCGCC ATTGTCCACA 
ATAACATCGA CGCCGGAGAA GGAGATCAAC ACATCCATAG CCTCGCTAGC GAGCACATCA 
AATAGTTCTC TAAGTGGTCC CAAGACGCCA GATCCTCATG TCCTGACAGA GGAGCTAACC 
CCTAAGCGAC CTCTGCGTTC GGAAATGGAG AAGGAACGTC GACTCTCTCG TCCTGCTAGC 
ATCGCTACGC CAACCGCCAG TATCAAGAAT TTCCCTGACA CGCGCTCCCT GTCGGAAAGC 
AGCAACCGCA ACTCTGTTGA AACCACCGAT TCCACTTCAG AGGAGGACAT TCGACCTCCG 
CCTTTGCCCG CCAAGGCACG TGACTCAACT GATTTTACCA GCCTGTCGCA AAACATGGAT 
TGGACCCCGA ATGGTTATGC TATGCTCAGC ACTATTAGCA ATACCAGCAG CATGAGCACC 
ACCTCAACCC TGACGAAGAC CAGTATTACC AATACCACGT ACGAGTATTT GGAGACCACG 
AACTTCAGTT TGGTGGGTGC CATCGATGGA AACAAACCGC GTCCGCCGAC GCCGCCACCG 
AAGCCATCGC GCCACAGCAA ACACATTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477144.1
CDS	576..6488
Translation	MSVWSDCNAKQAEFGIAKCNFDQESKPHRLNLDVGDAVIILKETTHWYYGYRQKAKEIRGIFPKSYIHLCEYNIVNGEYCIQRTDIVEEITKVLLEWGSIAKDYFLTTNPSFPKIRRKMNELNNNRAALISGNLPLDEVRKVKLLATNQIDTGNKLLGLDMVVRDESGDILDTNAICTTELYEQHMHAVQRIDKANRLSSERGTTRTPNKYSHNILLHVNAFVCKFQEDSDLLFTLFDGETHKPISENYVVKWSRTGIARDTDQIDNNRVLFTDLSKSDLAIAKMYLVCYAIRIGSMEFKDSAESKRTSMSIANSMLNASSRKASQLSVSSSGSSSSNGEYIIRRPFGVACKDLTPFINKSDDFRGNIDLPFIMCEKETLDGTLRKLIANKDIGKIDSKMAVTIEVLRGDIKQIKEEFPRLMHTNVPVARKMGFPEVILPGDVRNDLYLTICSGEFARIAKTSEKNVEVSVCVANEQGYLMPGVLSIGAGHQPIDEYKSVVYYHDDKPKWQETFKIHVPIEDFKQCHLRFVLKHRSSNEQKDRTEKPFGLAYVRLMQANGTTITQGQHILAVYKIDHKKYDKTVANCYLELPATVAELQGAKPSIGGLTLLPKDQLSIGVNLCSTKLTQSVSLLGLLNWSAHKETLEQSLNALSTVPGEEVVKFLQDILDALFNILVENDHPEKYDQLVFMSIIHLIETVSDLKYQHFLSVLDVYINESFSFTLAYTKLMDVLQKNISEAISPKEKSADGNDLEESPEVRRLYKTTRYLHYVMKFVIRSRVLYAEMNCNTDYMDFATRLQELLRMFIDMIGCPSNLLKSEGALLKNLHIIATDLMQVFEHVRLSISIVEILEKFPPRRLTQSKMGCIKDFVETKLFTLPKCRAILLPVFCKHIKDHLESKEEIAECINIMNNILKLLFRSDVGSTHNDIRDIMIILFRTVMKAAHALDRDTGLVGKFFAIMLGILQRMDAQHYEYFVKDLHQSGELKHFVIEILLVFEELVSPHQKAVFPRDWMDMIMHQNTVILGALKHLTVVITDYFLCPFEKQIWSNFFQCSIAFLVQSPLQLNDFNDNKRQIVFARYRDIRKDTAMEIRKMWFQLGQHKPKFVPQLVEPILEMSMIPEKELRQETIPIFFDMMQCEYYSSRLEHESYGDTKFNNAHHKGNFSDFKTAMIEKLDILIGAGKGDAEYKHLFETIMLERCAAHNTLNVDGTAFVQMVTRLMDKLLEYRFIIQDESKENRMACTFSLLQFYSEVDLKEMYIRYVNKLCALHMEFENYTEAAFTLKLHTELLRWTDTELSHQLRSYRHNNCRTHRQLKEALYFEIMEYFDKGKQWECAIDMCRVLARQYEEEIFDYLKLAELLNRMALFYEKIIKELRHNSEYFRVCFYGRGFPRFLQNRVYIFRGKEYERHSDFCARMLVQHPQAELMQTLEAPGDDITNSDGQYIQVNKVEPIMGQAFNKFNDKIINNEIVKYFTANNVQKFQFSRPFRDSTNGGDRDDVRNLWLERTELRISYPLPGILRWFPVVETNTFKISPLERAVEIMKDTNRDIRQLVILHKSDETLHINPLSMKLNGIVDPAVMGGFAKYEEAFLTDDYLEQNPDDKELVEELKELIANQIPLLDLAIQLHRLRAPDSLKALQEHLERCFADMQQHVEQRYGRKSCDLKIERDSVVMRRPNSFLPSLFDGSNNRHSETSMGSSDSGLSKSTFLPRPQTNSIKNPFSGLSFNTRPSLGHSPSIKSNKSKDKTPSKRRTKDGKVKEREAHSLSSSQWYTPPLSTITSTPEKEINTSIASLASTSNSSLSGPKTPDPHVLTEELTPKRPLRSEMEKERRLSRPASIATPTASIKNFPDTRSLSESSNRNSVETTDSTSEEDIRPPPLPAKARDSTDFTSLSQNMDWTPNGYAMLSTISNTSSMSTTSTLTKTSITNTTYEYLETTNFSLVGAIDGNKPRPPTPPPKPSRHSKHIP

Target ORF information:

RefSeq Version	NM_057796.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myoblast city (mbc), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057796.4

ORF Insert Sequence:

ATGAGTGTGT GGAGCGACTG CAATGCCAAG CAGGCAGAAT TCGGCATTGC CAAGTGCAAT 
TTCGACCAGG AGTCGAAGCC ACATCGCCTT AATCTGGATG TGGGTGATGC GGTCATAATT 
CTTAAGGAGA CCACCCATTG GTACTACGGC TACAGACAAA AAGCAAAGGA AATACGCGGC 
ATATTTCCCA AGAGCTATAT ACACTTATGC GAATATAATA TCGTGAATGG GGAGTACTGC 
ATCCAGCGCA CGGATATTGT CGAGGAGATC ACTAAAGTTC TTCTCGAGTG GGGCTCCATA 
GCCAAGGATT ACTTTCTGAC CACAAATCCC AGCTTTCCCA AAATTCGACG AAAAATGAAC 
GAATTAAATA ATAATAGGGC GGCTTTGATC TCCGGAAACC TGCCATTGGA CGAGGTGCGA 
AAGGTGAAGC TGCTGGCCAC CAATCAGATC GATACTGGCA ACAAGCTCCT TGGCCTGGAC 
ATGGTGGTTC GCGACGAGAG CGGAGACATC TTGGACACCA ATGCCATCTG CACCACCGAG 
CTCTACGAGC AACATATGCA CGCTGTTCAG CGCATCGACA AGGCCAATCG GCTGTCAAGC 
GAGCGGGGAA CTACAAGAAC ACCTAACAAG TACTCTCACA ACATCCTGCT ACACGTGAAC 
GCCTTCGTTT GCAAGTTCCA GGAGGACTCT GACCTGCTCT TCACGCTTTT CGACGGCGAA 
ACACACAAAC CCATTAGCGA GAACTATGTG GTCAAGTGGT CACGGACAGG TATTGCGCGT 
GATACTGACC AGATCGACAA CAACCGCGTG CTGTTCACGG ATCTCAGTAA GAGCGACCTA 
GCCATTGCCA AAATGTACCT GGTGTGCTAT GCCATCCGAA TCGGTTCCAT GGAGTTTAAG 
GATTCTGCCG AGTCGAAACG GACCAGCATG AGCATTGCCA ACAGCATGCT GAACGCCTCC 
AGTCGCAAGG CCTCCCAGCT ATCGGTGAGC TCCAGTGGAT CGTCCAGCAG CAATGGGGAA 
TACATCATTC GACGTCCGTT TGGTGTGGCC TGCAAGGACC TCACGCCCTT CATCAATAAG 
TCGGACGACT TCCGAGGCAA CATAGACCTG CCCTTCATCA TGTGCGAAAA GGAAACGCTG 
GACGGAACGC TGCGGAAGCT AATCGCGAAT AAGGATATTG GCAAGATTGA CTCCAAGATG 
GCTGTAACTA TCGAGGTGCT TCGGGGCGAC ATCAAGCAGA TAAAGGAAGA ATTTCCACGC 
TTGATGCACA CAAATGTGCC AGTTGCCCGA AAAATGGGAT TTCCTGAGGT CATTTTGCCA 
GGCGATGTGC GAAATGATTT GTACCTTACC ATCTGCAGTG GAGAATTCGC ACGCATTGCC 
AAGACCTCGG AGAAGAACGT TGAGGTGTCG GTGTGTGTGG CCAATGAGCA GGGATACCTT 
ATGCCCGGTG TGCTAAGCAT TGGAGCTGGA CATCAACCCA TTGATGAGTA CAAATCGGTT 
GTATACTACC ACGATGACAA GCCTAAGTGG CAGGAGACCT TCAAGATCCA CGTGCCCATT 
GAAGACTTTA AACAATGTCA TTTGCGCTTT GTGCTCAAAC ATCGTAGCAG TAATGAGCAG 
AAGGATCGAA CTGAAAAACC CTTCGGTCTC GCGTATGTGC GTCTAATGCA GGCCAACGGG 
ACAACCATCA CGCAGGGTCA ACATATCCTG GCCGTTTACA AAATTGATCA CAAAAAGTAC 
GACAAAACAG TAGCCAATTG TTATTTAGAA CTTCCAGCCA CGGTTGCAGA GCTGCAAGGT 
GCAAAGCCTT CTATCGGTGG ACTTACTCTC TTGCCAAAGG ATCAGCTATC GATTGGGGTT 
AACCTGTGCA GCACCAAGCT CACGCAGAGC GTGAGCTTGC TGGGACTTCT GAATTGGTCG 
GCGCACAAGG AGACGCTGGA ACAGTCGCTA AACGCCTTGT CCACAGTGCC TGGAGAGGAG 
GTGGTGAAGT TCCTGCAGGA CATACTCGAT GCCTTGTTTA ATATACTGGT GGAGAATGAT 
CATCCTGAGA AATATGACCA GCTCGTCTTT ATGAGCATTA TACATTTGAT TGAAACGGTG 
TCCGATCTCA AGTACCAACA CTTTCTCAGT GTTCTCGATG TGTACATTAA TGAAAGCTTC 
TCGTTTACAC TTGCCTACAC CAAATTGATG GATGTGCTGC AGAAGAATAT TAGTGAAGCG 
ATCTCACCAA AAGAAAAGTC TGCTGATGGC AATGATCTGG AGGAGAGTCC AGAGGTGCGC 
CGCCTGTACA AGACAACTCG TTACCTTCAC TATGTGATGA AGTTCGTGAT TCGATCGCGT 
GTGCTCTACG CCGAGATGAA CTGCAACACG GACTATATGG ACTTTGCTAC CCGGCTGCAG 
GAGCTTCTTC GCATGTTCAT CGACATGATT GGCTGTCCGA GCAATCTGCT TAAATCGGAG 
GGAGCGCTGC TCAAGAATCT GCACATCATC GCCACAGATT TAATGCAGGT CTTTGAACAT 
GTGCGCTTAA GCATATCCAT TGTGGAAATC CTTGAGAAAT TCCCGCCGCG ACGTCTTACT 
CAGTCTAAGA TGGGATGCAT TAAAGATTTT GTGGAGACAA AACTGTTTAC GTTGCCCAAG 
TGTCGGGCCA TCCTACTGCC CGTGTTCTGC AAACACATCA AGGATCACCT CGAGAGCAAG 
GAGGAGATTG CCGAGTGCAT CAACATCATG AACAACATAC TGAAACTTCT GTTCCGATCG 
GATGTGGGTT CCACGCACAA TGACATTCGA GACATTATGA TCATCCTGTT TCGCACTGTG 
ATGAAGGCAG CTCACGCCCT GGACAGGGAC ACGGGGTTGG TGGGCAAATT CTTTGCCATT 
ATGCTGGGTA TACTGCAGCG CATGGACGCC CAGCACTACG AGTACTTTGT GAAGGACCTG 
CACCAGAGCG GCGAGTTGAA ACACTTTGTC ATAGAGATCC TTCTGGTATT TGAGGAGCTG 
GTGTCTCCGC ACCAGAAAGC AGTGTTTCCG CGGGACTGGA TGGACATGAT TATGCACCAG 
AATACTGTGA TCCTTGGTGC TCTGAAGCAC CTCACCGTGG TCATTACGGA CTACTTCTTG 
TGCCCGTTCG AGAAACAGAT CTGGTCGAAC TTCTTCCAGT GCTCTATTGC CTTTCTGGTG 
CAATCCCCGC TTCAATTGAA TGATTTCAAC GATAATAAAC GGCAAATTGT GTTCGCAAGA 
TATCGTGACA TTCGCAAGGA TACGGCCATG GAGATCAGGA AGATGTGGTT CCAGCTGGGG 
CAGCACAAAC CCAAGTTTGT GCCGCAGTTG GTGGAGCCAA TACTGGAAAT GAGCATGATA 
CCGGAGAAGG AGCTGCGCCA GGAGACCATT CCTATCTTCT TCGACATGAT GCAATGCGAG 
TACTATAGCT CGCGATTGGA ACACGAAAGT TACGGCGACA CCAAATTCAA CAATGCCCAC 
CACAAGGGTA ACTTTTCCGA CTTCAAGACA GCGATGATTG AGAAGTTGGA CATCCTGATT 
GGAGCTGGTA AAGGCGACGC TGAATACAAG CATCTGTTCG AGACAATTAT GCTAGAGCGC 
TGCGCTGCCC ACAACACTCT TAACGTGGAT GGCACTGCCT TTGTGCAGAT GGTAACCCGG 
CTGATGGACA AGTTGTTGGA GTATCGCTTT ATAATCCAGG ATGAGAGCAA GGAGAATCGC 
ATGGCTTGCA CGTTCTCTCT GTTGCAGTTC TATTCGGAGG TGGATCTCAA GGAGATGTAC 
ATACGTTATG TCAACAAACT GTGCGCTCTG CACATGGAGT TCGAGAACTA CACGGAGGCG 
GCGTTCACGC TTAAGCTGCA CACGGAACTG CTGCGTTGGA CGGATACGGA GCTGTCGCAC 
CAACTGCGCA GCTATCGGCA TAATAACTGC CGTACTCACC GGCAACTGAA GGAGGCTCTT 
TACTTCGAGA TCATGGAGTA CTTCGACAAG GGCAAACAGT GGGAGTGCGC CATCGATATG 
TGCAGGGTGC TGGCCCGCCA GTACGAGGAG GAGATCTTCG ACTATCTCAA GCTGGCGGAA 
CTTCTGAATC GCATGGCATT GTTCTATGAG AAGATCATCA AGGAGCTGCG CCACAATTCT 
GAATATTTCC GCGTGTGCTT CTATGGGCGT GGATTCCCGC GGTTTCTTCA GAACCGTGTC 
TACATCTTCC GTGGCAAGGA GTACGAGCGG CACAGTGACT TCTGCGCCCG TATGCTAGTA 
CAGCATCCAC AGGCTGAGCT CATGCAAACG CTGGAGGCTC CGGGCGACGA CATCACCAAC 
AGTGATGGTC AGTACATACA GGTGAACAAG GTGGAGCCGA TAATGGGTCA GGCGTTCAAC 
AAGTTCAACG ATAAGATTAT CAACAACGAA ATCGTGAAAT ACTTCACCGC CAACAACGTG 
CAAAAGTTTC AGTTTTCACG TCCCTTCCGG GACAGCACGA ACGGTGGCGA TAGGGACGAT 
GTACGAAATC TGTGGCTGGA GCGCACAGAG CTGCGTATCA GCTACCCACT TCCGGGTATT 
CTGCGCTGGT TCCCCGTCGT TGAGACCAAT ACTTTTAAAA TCTCTCCCCT AGAGCGGGCG 
GTCGAAATTA TGAAAGATAC GAACCGTGAC ATTCGACAAC TGGTGATCCT GCACAAGAGC 
GACGAAACTC TGCACATTAA TCCGCTGAGT ATGAAACTTA ATGGCATTGT GGATCCGGCT 
GTAATGGGTG GCTTTGCCAA GTACGAGGAA GCCTTCCTAA CCGACGACTA CTTGGAGCAG 
AATCCGGACG ACAAGGAGTT GGTAGAAGAG CTCAAAGAAC TGATTGCGAA CCAGATTCCA 
CTGCTCGATT TAGCAATTCA GCTTCATCGC TTGCGGGCAC CAGATAGTTT GAAAGCGCTG 
CAGGAACACC TGGAGCGTTG CTTCGCCGAC ATGCAGCAGC ATGTGGAGCA GCGCTATGGT 
CGCAAGTCCT GCGACCTCAA AATCGAACGG GATTCCGTGG TGATGCGACG TCCAAACTCC 
TTTCTGCCTT CGCTTTTCGA TGGCAGCAAC AATCGACACT CCGAAACCAG CATGGGATCT 
TCAGACAGCG GCCTGTCAAA GTCAACGTTT CTGCCGCGTC CACAGACGAA TTCTATTAAA 
AACCCATTTT CCGGCCTAAG TTTCAACACA AGGCCCAGTT TGGGACACTC GCCGAGCATT 
AAGAGCAACA AGAGCAAGGA TAAGACTCCG AGTAAGCGAA GAACCAAGGA TGGAAAGGTT 
AAGGAGCGTG AAGCCCATAG CCTGTCCAGC AGTCAGTGGT ACACACCGCC ATTGTCCACA 
ATAACATCGA CGCCGGAGAA GGAGATCAAC ACATCCATAG CCTCGCTAGC GAGCACATCA 
AATAGTTCTC TAAGTGGTCC CAAGACGCCA GATCCTCATG TCCTGACAGA GGAGCTAACC 
CCTAAGCGAC CTCTGCGTTC GGAAATGGAG AAGGAACGTC GACTCTCTCG TCCTGCTAGC 
ATCGCTACGC CAACCGCCAG TATCAAGAAT TTCCCTGACA CGCGCTCCCT GTCGGAAAGC 
AGCAACCGCA ACTCTGTTGA AACCACCGAT TCCACTTCAG AGGAGGACAT TCGACCTCCG 
CCTTTGCCCG CCAAGGCACG TGACTCAACT GATTTTACCA GCCTGTCGCA AAACATGGAT 
TGGACCCCGA ATGGTTATGC TATGCTCAGC ACTATTAGCA ATACCAGCAG CATGAGCACC 
ACCTCAACCC TGACGAAGAC CAGTATTACC AATACCACGT ACGAGTATTT GGAGACCACG 
AACTTCAGTT TGGTGGGTGC CATCGATGGA AACAAACCGC GTCCGCCGAC GCCGCCACCG 
AAGCCATCGC GCCACAGCAA ACACATTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mbc ( NM_001260338.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247267.1 Drosophila melanogaster myoblast city (mbc), transcript variant B, mRNA.

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CloneID	OFa08001
Clone ID Related Accession (Same CDS sequence)	NM_001260338.2
Accession Version	NM_001260338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	myoblast city, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260338.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTGT GGAGCGACTG CAATGCCAAG CAGGCAGAAT TCGGCATTGC CAAGTGCAAT 
TTCGACCAGG AGTCGAAGCC ACATCGCCTT AATCTGGATG TGGGTGATGC GGTCATAATT 
CTTAAGGAGA CCACCCATTG GTACTACGGC TACAGACAAA AAGCAAAGGA AATACGCGGC 
ATATTTCCCA AGAGCTATAT ACACTTATGC GAATATAATA TCGTGAATGG GGAGTACTGC 
ATCCAGCGCA CGGATATTGT CGAGGAGATC ACTAAAGTTC TTCTCGAGTG GGGCTCCATA 
GCCAAGGATT ACTTTCTGAC CACAAATCCC AGCTTTCCCA AAATTCGACG AAAAATGAAC 
GAATTAAATA ATAATAGGGC GGCTTTGATC TCCGGAAACC TGCCATTGGA CGAGGTGCGA 
AAGGTGAAGC TGCTGGCCAC CAATCAGATC GATACTGGCA ACAAGCTCCT TGGCCTGGAC 
ATGGTGGTTC GCGACGAGAG CGGAGACATC TTGGACACCA ATGCCATCTG CACCACCGAG 
CTCTACGAGC AACATATGCA CGCTGTTCAG CGCATCGACA AGGCCAATCG GCTGTCAAGC 
GAGCGGGGAA CTACAAGAAC ACCTAACAAG TACTCTCACA ACATCCTGCT ACACGTGAAC 
GCCTTCGTTT GCAAGTTCCA GGAGGACTCT GACCTGCTCT TCACGCTTTT CGACGGCGAA 
ACACACAAAC CCATTAGCGA GAACTATGTG GTCAAGTGGT CACGGACAGG TATTGCGCGT 
GATACTGACC AGATCGACAA CAACCGCGTG CTGTTCACGG ATCTCAGTAA GAGCGACCTA 
GCCATTGCCA AAATGTACCT GGTGTGCTAT GCCATCCGAA TCGGTTCCAT GGAGTTTAAG 
GATTCTGCCG AGTCGAAACG GACCAGCATG AGCATTGCCA ACAGCATGCT GAACGCCTCC 
AGTCGCAAGG CCTCCCAGCT ATCGGTGAGC TCCAGTGGAT CGTCCAGCAG CAATGGGGAA 
TACATCATTC GACGTCCGTT TGGTGTGGCC TGCAAGGACC TCACGCCCTT CATCAATAAG 
TCGGACGACT TCCGAGGCAA CATAGACCTG CCCTTCATCA TGTGCGAAAA GGAAACGCTG 
GACGGAACGC TGCGGAAGCT AATCGCGAAT AAGGATATTG GCAAGATTGA CTCCAAGATG 
GCTGTAACTA TCGAGGTGCT TCGGGGCGAC ATCAAGCAGA TAAAGGAAGA ATTTCCACGC 
TTGATGCACA CAAATGTGCC AGTTGCCCGA AAAATGGGAT TTCCTGAGGT CATTTTGCCA 
GGCGATGTGC GAAATGATTT GTACCTTACC ATCTGCAGTG GAGAATTCGC ACGCATTGCC 
AAGACCTCGG AGAAGAACGT TGAGGTGTCG GTGTGTGTGG CCAATGAGCA GGGATACCTT 
ATGCCCGGTG TGCTAAGCAT TGGAGCTGGA CATCAACCCA TTGATGAGTA CAAATCGGTT 
GTATACTACC ACGATGACAA GCCTAAGTGG CAGGAGACCT TCAAGATCCA CGTGCCCATT 
GAAGACTTTA AACAATGTCA TTTGCGCTTT GTGCTCAAAC ATCGTAGCAG TAATGAGCAG 
AAGGATCGAA CTGAAAAACC CTTCGGTCTC GCGTATGTGC GTCTAATGCA GGCCAACGGG 
ACAACCATCA CGCAGGGTCA ACATATCCTG GCCGTTTACA AAATTGATCA CAAAAAGTAC 
GACAAAACAG TAGCCAATTG TTATTTAGAA CTTCCAGCCA CGGTTGCAGA GCTGCAAGGT 
GCAAAGCCTT CTATCGGTGG ACTTACTCTC TTGCCAAAGG ATCAGCTATC GATTGGGGTT 
AACCTGTGCA GCACCAAGCT CACGCAGAGC GTGAGCTTGC TGGGACTTCT GAATTGGTCG 
GCGCACAAGG AGACGCTGGA ACAGTCGCTA AACGCCTTGT CCACAGTGCC TGGAGAGGAG 
GTGGTGAAGT TCCTGCAGGA CATACTCGAT GCCTTGTTTA ATATACTGGT GGAGAATGAT 
CATCCTGAGA AATATGACCA GCTCGTCTTT ATGAGCATTA TACATTTGAT TGAAACGGTG 
TCCGATCTCA AGTACCAACA CTTTCTCAGT GTTCTCGATG TGTACATTAA TGAAAGCTTC 
TCGTTTACAC TTGCCTACAC CAAATTGATG GATGTGCTGC AGAAGAATAT TAGTGAAGCG 
ATCTCACCAA AAGAAAAGTC TGCTGATGGC AATGATCTGG AGGAGAGTCC AGAGGTGCGC 
CGCCTGTACA AGACAACTCG TTACCTTCAC TATGTGATGA AGTTCGTGAT TCGATCGCGT 
GTGCTCTACG CCGAGATGAA CTGCAACACG GACTATATGG ACTTTGCTAC CCGGCTGCAG 
GAGCTTCTTC GCATGTTCAT CGACATGATT GGCTGTCCGA GCAATCTGCT TAAATCGGAG 
GGAGCGCTGC TCAAGAATCT GCACATCATC GCCACAGATT TAATGCAGGT CTTTGAACAT 
GTGCGCTTAA GCATATCCAT TGTGGAAATC CTTGAGAAAT TCCCGCCGCG ACGTCTTACT 
CAGTCTAAGA TGGGATGCAT TAAAGATTTT GTGGAGACAA AACTGTTTAC GTTGCCCAAG 
TGTCGGGCCA TCCTACTGCC CGTGTTCTGC AAACACATCA AGGATCACCT CGAGAGCAAG 
GAGGAGGGAG ACTCGAAAAC CGATATCTGG CAGCAAGAAA AGAATCTTTC GAAAGCCGCC 
AAAGTGCTTG GACAGAAAAA ATCCCAACTG CATACATGTG ATACGACTGC CAACAAAAAG 
ATTGCCGAGT GCATCAACAT CATGAACAAC ATACTGAAAC TTCTGTTCCG ATCGGATGTG 
GGTTCCACGC ACAATGACAT TCGAGACATT ATGATCATCC TGTTTCGCAC TGTGATGAAG 
GCAGCTCACG CCCTGGACAG GGACACGGGG TTGGTGGGCA AATTCTTTGC CATTATGCTG 
GGTATACTGC AGCGCATGGA CGCCCAGCAC TACGAGTACT TTGTGAAGGA CCTGCACCAG 
AGCGGCGAGT TGAAACACTT TGTCATAGAG ATCCTTCTGG TATTTGAGGA GCTGGTGTCT 
CCGCACCAGA AAGCAGTGTT TCCGCGGGAC TGGATGGACA TGATTATGCA CCAGAATACT 
GTGATCCTTG GTGCTCTGAA GCACCTCACC GTGGTCATTA CGGACTACTT CTTGTGCCCG 
TTCGAGAAAC AGATCTGGTC GAACTTCTTC CAGTGCTCTA TTGCCTTTCT GGTGCAATCC 
CCGCTTCAAT TGAATGATTT CAACGATAAT AAACGGCAAA TTGTGTTCGC AAGATATCGT 
GACATTCGCA AGGATACGGC CATGGAGATC AGGAAGATGT GGTTCCAGCT GGGGCAGCAC 
AAACCCAAGT TTGTGCCGCA GTTGGTGGAG CCAATACTGG AAATGAGCAT GATACCGGAG 
AAGGAGCTGC GCCAGGAGAC CATTCCTATC TTCTTCGACA TGATGCAATG CGAGTACTAT 
AGCTCGCGAT TGGAACACGA AAGTTACGGC GACACCAAAT TCAACAATGC CCACCACAAG 
GGTAACTTTT CCGACTTCAA GACAGCGATG ATTGAGAAGT TGGACATCCT GATTGGAGCT 
GGTAAAGGCG ACGCTGAATA CAAGCATCTG TTCGAGACAA TTATGCTAGA GCGCTGCGCT 
GCCCACAACA CTCTTAACGT GGATGGCACT GCCTTTGTGC AGATGGTAAC CCGGCTGATG 
GACAAGTTGT TGGAGTATCG CTTTATAATC CAGGATGAGA GCAAGGAGAA TCGCATGGCT 
TGCACGTTCT CTCTGTTGCA GTTCTATTCG GAGGTGGATC TCAAGGAGAT GTACATACGT 
TATGTCAACA AACTGTGCGC TCTGCACATG GAGTTCGAGA ACTACACGGA GGCGGCGTTC 
ACGCTTAAGC TGCACACGGA ACTGCTGCGT TGGACGGATA CGGAGCTGTC GCACCAACTG 
CGCAGCTATC GGCATAATAA CTGCCGTACT CACCGGCAAC TGAAGGAGGC TCTTTACTTC 
GAGATCATGG AGTACTTCGA CAAGGGCAAA CAGTGGGAGT GCGCCATCGA TATGTGCAGG 
GTGCTGGCCC GCCAGTACGA GGAGGAGATC TTCGACTATC TCAAGCTGGC GGAACTTCTG 
AATCGCATGG CATTGTTCTA TGAGAAGATC ATCAAGGAGC TGCGCCACAA TTCTGAATAT 
TTCCGCGTGT GCTTCTATGG GCGTGGATTC CCGCGGTTTC TTCAGAACCG TGTCTACATC 
TTCCGTGGCA AGGAGTACGA GCGGCACAGT GACTTCTGCG CCCGTATGCT AGTACAGCAT 
CCACAGGCTG AGCTCATGCA AACGCTGGAG GCTCCGGGCG ACGACATCAC CAACAGTGAT 
GGTCAGTACA TACAGGTGAA CAAGGTGGAG CCGATAATGG GTCAGGCGTT CAACAAGTTC 
AACGATAAGA TTATCAACAA CGAAATCGTG AAATACTTCA CCGCCAACAA CGTGCAAAAG 
TTTCAGTTTT CACGTCCCTT CCGGGACAGC ACGAACGGTG GCGATAGGGA CGATGTACGA 
AATCTGTGGC TGGAGCGCAC AGAGCTGCGT ATCAGCTACC CACTTCCGGG TATTCTGCGC 
TGGTTCCCCG TCGTTGAGAC CAATACTTTT AAAATCTCTC CCCTAGAGCG GGCGGTCGAA 
ATTATGAAAG ATACGAACCG TGACATTCGA CAACTGGTGA TCCTGCACAA GAGCGACGAA 
ACTCTGCACA TTAATCCGCT GAGTATGAAA CTTAATGGCA TTGTGGATCC GGCTGTAATG 
GGTGGCTTTG CCAAGTACGA GGAAGCCTTC CTAACCGACG ACTACTTGGA GCAGAATCCG 
GACGACAAGG AGTTGGTAGA AGAGCTCAAA GAACTGATTG CGAACCAGAT TCCACTGCTC 
GATTTAGCAA TTCAGCTTCA TCGCTTGCGG GCACCAGATA GTTTGAAAGC GCTGCAGGAA 
CACCTGGAGC GTTGCTTCGC CGACATGCAG CAGCATGTGG AGCAGCGCTA TGGTCGCAAG 
TCCTGCGACC TCAAAATCGA ACGGGATTCC GTGGTGATGC GACGTCCAAA CTCCTTTCTG 
CCTTCGCTTT TCGATGGCAG CAACAATCGA CACTCCGAAA CCAGCATGGG ATCTTCAGAC 
AGCGGCCTGT CAAAGTCAAC GTTTCTGCCG CGTCCACAGA CGAATTCTAT TAAAAACCCA 
TTTTCCGGCC TAAGTTTCAA CACAAGGCCC AGTTTGGGAC ACTCGCCGAG CATTAAGAGC 
AACAAGAGCA AGGATAAGAC TCCGAGTAAG CGAAGAACCA AGGATGGAAA GGTTAAGGAG 
CGTGAAGCCC ATAGCCTGTC CAGCAGTCAG TGGTACACAC CGCCATTGTC CACAATAACA 
TCGACGCCGG AGAAGGAGAT CAACACATCC ATAGCCTCGC TAGCGAGCAC ATCAAATAGT 
TCTCTAAGTG GTCCCAAGAC GCCAGATCCT CATGTCCTGA CAGAGGAGCT AACCCCTAAG 
CGACCTCTGC GTTCGGAAAT GGAGAAGGAA CGTCGACTCT CTCGTCCTGC TAGCATCGCT 
ACGCCAACCG CCAGTATCAA GAATTTCCCT GACACGCGCT CCCTGTCGGA AAGCAGCAAC 
CGCAACTCTG TTGAAACCAC CGATTCCACT TCAGAGGAGG ACATTCGACC TCCGCCTTTG 
CCCGCCAAGG CACGTGACTC AACTGATTTT ACCAGCCTGT CGCAAAACAT GGATTGGACC 
CCGAATGGTT ATGCTATGCT CAGCACTATT AGCAATACCA GCAGCATGAG CACCACCTCA 
ACCCTGACGA AGACCAGTAT TACCAATACC ACGTACGAGT ATTTGGAGAC CACGAACTTC 
AGTTTGGTGG GTGCCATCGA TGGAAACAAA CCGCGTCCGC CGACGCCGCC ACCGAAGCCA 
TCGCGCCACA GCAAACACAT TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247267.1
CDS	576..6602
Translation	MSVWSDCNAKQAEFGIAKCNFDQESKPHRLNLDVGDAVIILKETTHWYYGYRQKAKEIRGIFPKSYIHLCEYNIVNGEYCIQRTDIVEEITKVLLEWGSIAKDYFLTTNPSFPKIRRKMNELNNNRAALISGNLPLDEVRKVKLLATNQIDTGNKLLGLDMVVRDESGDILDTNAICTTELYEQHMHAVQRIDKANRLSSERGTTRTPNKYSHNILLHVNAFVCKFQEDSDLLFTLFDGETHKPISENYVVKWSRTGIARDTDQIDNNRVLFTDLSKSDLAIAKMYLVCYAIRIGSMEFKDSAESKRTSMSIANSMLNASSRKASQLSVSSSGSSSSNGEYIIRRPFGVACKDLTPFINKSDDFRGNIDLPFIMCEKETLDGTLRKLIANKDIGKIDSKMAVTIEVLRGDIKQIKEEFPRLMHTNVPVARKMGFPEVILPGDVRNDLYLTICSGEFARIAKTSEKNVEVSVCVANEQGYLMPGVLSIGAGHQPIDEYKSVVYYHDDKPKWQETFKIHVPIEDFKQCHLRFVLKHRSSNEQKDRTEKPFGLAYVRLMQANGTTITQGQHILAVYKIDHKKYDKTVANCYLELPATVAELQGAKPSIGGLTLLPKDQLSIGVNLCSTKLTQSVSLLGLLNWSAHKETLEQSLNALSTVPGEEVVKFLQDILDALFNILVENDHPEKYDQLVFMSIIHLIETVSDLKYQHFLSVLDVYINESFSFTLAYTKLMDVLQKNISEAISPKEKSADGNDLEESPEVRRLYKTTRYLHYVMKFVIRSRVLYAEMNCNTDYMDFATRLQELLRMFIDMIGCPSNLLKSEGALLKNLHIIATDLMQVFEHVRLSISIVEILEKFPPRRLTQSKMGCIKDFVETKLFTLPKCRAILLPVFCKHIKDHLESKEEGDSKTDIWQQEKNLSKAAKVLGQKKSQLHTCDTTANKKIAECINIMNNILKLLFRSDVGSTHNDIRDIMIILFRTVMKAAHALDRDTGLVGKFFAIMLGILQRMDAQHYEYFVKDLHQSGELKHFVIEILLVFEELVSPHQKAVFPRDWMDMIMHQNTVILGALKHLTVVITDYFLCPFEKQIWSNFFQCSIAFLVQSPLQLNDFNDNKRQIVFARYRDIRKDTAMEIRKMWFQLGQHKPKFVPQLVEPILEMSMIPEKELRQETIPIFFDMMQCEYYSSRLEHESYGDTKFNNAHHKGNFSDFKTAMIEKLDILIGAGKGDAEYKHLFETIMLERCAAHNTLNVDGTAFVQMVTRLMDKLLEYRFIIQDESKENRMACTFSLLQFYSEVDLKEMYIRYVNKLCALHMEFENYTEAAFTLKLHTELLRWTDTELSHQLRSYRHNNCRTHRQLKEALYFEIMEYFDKGKQWECAIDMCRVLARQYEEEIFDYLKLAELLNRMALFYEKIIKELRHNSEYFRVCFYGRGFPRFLQNRVYIFRGKEYERHSDFCARMLVQHPQAELMQTLEAPGDDITNSDGQYIQVNKVEPIMGQAFNKFNDKIINNEIVKYFTANNVQKFQFSRPFRDSTNGGDRDDVRNLWLERTELRISYPLPGILRWFPVVETNTFKISPLERAVEIMKDTNRDIRQLVILHKSDETLHINPLSMKLNGIVDPAVMGGFAKYEEAFLTDDYLEQNPDDKELVEELKELIANQIPLLDLAIQLHRLRAPDSLKALQEHLERCFADMQQHVEQRYGRKSCDLKIERDSVVMRRPNSFLPSLFDGSNNRHSETSMGSSDSGLSKSTFLPRPQTNSIKNPFSGLSFNTRPSLGHSPSIKSNKSKDKTPSKRRTKDGKVKEREAHSLSSSQWYTPPLSTITSTPEKEINTSIASLASTSNSSLSGPKTPDPHVLTEELTPKRPLRSEMEKERRLSRPASIATPTASIKNFPDTRSLSESSNRNSVETTDSTSEEDIRPPPLPAKARDSTDFTSLSQNMDWTPNGYAMLSTISNTSSMSTTSTLTKTSITNTTYEYLETTNFSLVGAIDGNKPRPPTPPPKPSRHSKHIP

Target ORF information:

RefSeq Version	NM_001260338.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myoblast city (mbc), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260338.2

ORF Insert Sequence:

ATGAGTGTGT GGAGCGACTG CAATGCCAAG CAGGCAGAAT TCGGCATTGC CAAGTGCAAT 
TTCGACCAGG AGTCGAAGCC ACATCGCCTT AATCTGGATG TGGGTGATGC GGTCATAATT 
CTTAAGGAGA CCACCCATTG GTACTACGGC TACAGACAAA AAGCAAAGGA AATACGCGGC 
ATATTTCCCA AGAGCTATAT ACACTTATGC GAATATAATA TCGTGAATGG GGAGTACTGC 
ATCCAGCGCA CGGATATTGT CGAGGAGATC ACTAAAGTTC TTCTCGAGTG GGGCTCCATA 
GCCAAGGATT ACTTTCTGAC CACAAATCCC AGCTTTCCCA AAATTCGACG AAAAATGAAC 
GAATTAAATA ATAATAGGGC GGCTTTGATC TCCGGAAACC TGCCATTGGA CGAGGTGCGA 
AAGGTGAAGC TGCTGGCCAC CAATCAGATC GATACTGGCA ACAAGCTCCT TGGCCTGGAC 
ATGGTGGTTC GCGACGAGAG CGGAGACATC TTGGACACCA ATGCCATCTG CACCACCGAG 
CTCTACGAGC AACATATGCA CGCTGTTCAG CGCATCGACA AGGCCAATCG GCTGTCAAGC 
GAGCGGGGAA CTACAAGAAC ACCTAACAAG TACTCTCACA ACATCCTGCT ACACGTGAAC 
GCCTTCGTTT GCAAGTTCCA GGAGGACTCT GACCTGCTCT TCACGCTTTT CGACGGCGAA 
ACACACAAAC CCATTAGCGA GAACTATGTG GTCAAGTGGT CACGGACAGG TATTGCGCGT 
GATACTGACC AGATCGACAA CAACCGCGTG CTGTTCACGG ATCTCAGTAA GAGCGACCTA 
GCCATTGCCA AAATGTACCT GGTGTGCTAT GCCATCCGAA TCGGTTCCAT GGAGTTTAAG 
GATTCTGCCG AGTCGAAACG GACCAGCATG AGCATTGCCA ACAGCATGCT GAACGCCTCC 
AGTCGCAAGG CCTCCCAGCT ATCGGTGAGC TCCAGTGGAT CGTCCAGCAG CAATGGGGAA 
TACATCATTC GACGTCCGTT TGGTGTGGCC TGCAAGGACC TCACGCCCTT CATCAATAAG 
TCGGACGACT TCCGAGGCAA CATAGACCTG CCCTTCATCA TGTGCGAAAA GGAAACGCTG 
GACGGAACGC TGCGGAAGCT AATCGCGAAT AAGGATATTG GCAAGATTGA CTCCAAGATG 
GCTGTAACTA TCGAGGTGCT TCGGGGCGAC ATCAAGCAGA TAAAGGAAGA ATTTCCACGC 
TTGATGCACA CAAATGTGCC AGTTGCCCGA AAAATGGGAT TTCCTGAGGT CATTTTGCCA 
GGCGATGTGC GAAATGATTT GTACCTTACC ATCTGCAGTG GAGAATTCGC ACGCATTGCC 
AAGACCTCGG AGAAGAACGT TGAGGTGTCG GTGTGTGTGG CCAATGAGCA GGGATACCTT 
ATGCCCGGTG TGCTAAGCAT TGGAGCTGGA CATCAACCCA TTGATGAGTA CAAATCGGTT 
GTATACTACC ACGATGACAA GCCTAAGTGG CAGGAGACCT TCAAGATCCA CGTGCCCATT 
GAAGACTTTA AACAATGTCA TTTGCGCTTT GTGCTCAAAC ATCGTAGCAG TAATGAGCAG 
AAGGATCGAA CTGAAAAACC CTTCGGTCTC GCGTATGTGC GTCTAATGCA GGCCAACGGG 
ACAACCATCA CGCAGGGTCA ACATATCCTG GCCGTTTACA AAATTGATCA CAAAAAGTAC 
GACAAAACAG TAGCCAATTG TTATTTAGAA CTTCCAGCCA CGGTTGCAGA GCTGCAAGGT 
GCAAAGCCTT CTATCGGTGG ACTTACTCTC TTGCCAAAGG ATCAGCTATC GATTGGGGTT 
AACCTGTGCA GCACCAAGCT CACGCAGAGC GTGAGCTTGC TGGGACTTCT GAATTGGTCG 
GCGCACAAGG AGACGCTGGA ACAGTCGCTA AACGCCTTGT CCACAGTGCC TGGAGAGGAG 
GTGGTGAAGT TCCTGCAGGA CATACTCGAT GCCTTGTTTA ATATACTGGT GGAGAATGAT 
CATCCTGAGA AATATGACCA GCTCGTCTTT ATGAGCATTA TACATTTGAT TGAAACGGTG 
TCCGATCTCA AGTACCAACA CTTTCTCAGT GTTCTCGATG TGTACATTAA TGAAAGCTTC 
TCGTTTACAC TTGCCTACAC CAAATTGATG GATGTGCTGC AGAAGAATAT TAGTGAAGCG 
ATCTCACCAA AAGAAAAGTC TGCTGATGGC AATGATCTGG AGGAGAGTCC AGAGGTGCGC 
CGCCTGTACA AGACAACTCG TTACCTTCAC TATGTGATGA AGTTCGTGAT TCGATCGCGT 
GTGCTCTACG CCGAGATGAA CTGCAACACG GACTATATGG ACTTTGCTAC CCGGCTGCAG 
GAGCTTCTTC GCATGTTCAT CGACATGATT GGCTGTCCGA GCAATCTGCT TAAATCGGAG 
GGAGCGCTGC TCAAGAATCT GCACATCATC GCCACAGATT TAATGCAGGT CTTTGAACAT 
GTGCGCTTAA GCATATCCAT TGTGGAAATC CTTGAGAAAT TCCCGCCGCG ACGTCTTACT 
CAGTCTAAGA TGGGATGCAT TAAAGATTTT GTGGAGACAA AACTGTTTAC GTTGCCCAAG 
TGTCGGGCCA TCCTACTGCC CGTGTTCTGC AAACACATCA AGGATCACCT CGAGAGCAAG 
GAGGAGGGAG ACTCGAAAAC CGATATCTGG CAGCAAGAAA AGAATCTTTC GAAAGCCGCC 
AAAGTGCTTG GACAGAAAAA ATCCCAACTG CATACATGTG ATACGACTGC CAACAAAAAG 
ATTGCCGAGT GCATCAACAT CATGAACAAC ATACTGAAAC TTCTGTTCCG ATCGGATGTG 
GGTTCCACGC ACAATGACAT TCGAGACATT ATGATCATCC TGTTTCGCAC TGTGATGAAG 
GCAGCTCACG CCCTGGACAG GGACACGGGG TTGGTGGGCA AATTCTTTGC CATTATGCTG 
GGTATACTGC AGCGCATGGA CGCCCAGCAC TACGAGTACT TTGTGAAGGA CCTGCACCAG 
AGCGGCGAGT TGAAACACTT TGTCATAGAG ATCCTTCTGG TATTTGAGGA GCTGGTGTCT 
CCGCACCAGA AAGCAGTGTT TCCGCGGGAC TGGATGGACA TGATTATGCA CCAGAATACT 
GTGATCCTTG GTGCTCTGAA GCACCTCACC GTGGTCATTA CGGACTACTT CTTGTGCCCG 
TTCGAGAAAC AGATCTGGTC GAACTTCTTC CAGTGCTCTA TTGCCTTTCT GGTGCAATCC 
CCGCTTCAAT TGAATGATTT CAACGATAAT AAACGGCAAA TTGTGTTCGC AAGATATCGT 
GACATTCGCA AGGATACGGC CATGGAGATC AGGAAGATGT GGTTCCAGCT GGGGCAGCAC 
AAACCCAAGT TTGTGCCGCA GTTGGTGGAG CCAATACTGG AAATGAGCAT GATACCGGAG 
AAGGAGCTGC GCCAGGAGAC CATTCCTATC TTCTTCGACA TGATGCAATG CGAGTACTAT 
AGCTCGCGAT TGGAACACGA AAGTTACGGC GACACCAAAT TCAACAATGC CCACCACAAG 
GGTAACTTTT CCGACTTCAA GACAGCGATG ATTGAGAAGT TGGACATCCT GATTGGAGCT 
GGTAAAGGCG ACGCTGAATA CAAGCATCTG TTCGAGACAA TTATGCTAGA GCGCTGCGCT 
GCCCACAACA CTCTTAACGT GGATGGCACT GCCTTTGTGC AGATGGTAAC CCGGCTGATG 
GACAAGTTGT TGGAGTATCG CTTTATAATC CAGGATGAGA GCAAGGAGAA TCGCATGGCT 
TGCACGTTCT CTCTGTTGCA GTTCTATTCG GAGGTGGATC TCAAGGAGAT GTACATACGT 
TATGTCAACA AACTGTGCGC TCTGCACATG GAGTTCGAGA ACTACACGGA GGCGGCGTTC 
ACGCTTAAGC TGCACACGGA ACTGCTGCGT TGGACGGATA CGGAGCTGTC GCACCAACTG 
CGCAGCTATC GGCATAATAA CTGCCGTACT CACCGGCAAC TGAAGGAGGC TCTTTACTTC 
GAGATCATGG AGTACTTCGA CAAGGGCAAA CAGTGGGAGT GCGCCATCGA TATGTGCAGG 
GTGCTGGCCC GCCAGTACGA GGAGGAGATC TTCGACTATC TCAAGCTGGC GGAACTTCTG 
AATCGCATGG CATTGTTCTA TGAGAAGATC ATCAAGGAGC TGCGCCACAA TTCTGAATAT 
TTCCGCGTGT GCTTCTATGG GCGTGGATTC CCGCGGTTTC TTCAGAACCG TGTCTACATC 
TTCCGTGGCA AGGAGTACGA GCGGCACAGT GACTTCTGCG CCCGTATGCT AGTACAGCAT 
CCACAGGCTG AGCTCATGCA AACGCTGGAG GCTCCGGGCG ACGACATCAC CAACAGTGAT 
GGTCAGTACA TACAGGTGAA CAAGGTGGAG CCGATAATGG GTCAGGCGTT CAACAAGTTC 
AACGATAAGA TTATCAACAA CGAAATCGTG AAATACTTCA CCGCCAACAA CGTGCAAAAG 
TTTCAGTTTT CACGTCCCTT CCGGGACAGC ACGAACGGTG GCGATAGGGA CGATGTACGA 
AATCTGTGGC TGGAGCGCAC AGAGCTGCGT ATCAGCTACC CACTTCCGGG TATTCTGCGC 
TGGTTCCCCG TCGTTGAGAC CAATACTTTT AAAATCTCTC CCCTAGAGCG GGCGGTCGAA 
ATTATGAAAG ATACGAACCG TGACATTCGA CAACTGGTGA TCCTGCACAA GAGCGACGAA 
ACTCTGCACA TTAATCCGCT GAGTATGAAA CTTAATGGCA TTGTGGATCC GGCTGTAATG 
GGTGGCTTTG CCAAGTACGA GGAAGCCTTC CTAACCGACG ACTACTTGGA GCAGAATCCG 
GACGACAAGG AGTTGGTAGA AGAGCTCAAA GAACTGATTG CGAACCAGAT TCCACTGCTC 
GATTTAGCAA TTCAGCTTCA TCGCTTGCGG GCACCAGATA GTTTGAAAGC GCTGCAGGAA 
CACCTGGAGC GTTGCTTCGC CGACATGCAG CAGCATGTGG AGCAGCGCTA TGGTCGCAAG 
TCCTGCGACC TCAAAATCGA ACGGGATTCC GTGGTGATGC GACGTCCAAA CTCCTTTCTG 
CCTTCGCTTT TCGATGGCAG CAACAATCGA CACTCCGAAA CCAGCATGGG ATCTTCAGAC 
AGCGGCCTGT CAAAGTCAAC GTTTCTGCCG CGTCCACAGA CGAATTCTAT TAAAAACCCA 
TTTTCCGGCC TAAGTTTCAA CACAAGGCCC AGTTTGGGAC ACTCGCCGAG CATTAAGAGC 
AACAAGAGCA AGGATAAGAC TCCGAGTAAG CGAAGAACCA AGGATGGAAA GGTTAAGGAG 
CGTGAAGCCC ATAGCCTGTC CAGCAGTCAG TGGTACACAC CGCCATTGTC CACAATAACA 
TCGACGCCGG AGAAGGAGAT CAACACATCC ATAGCCTCGC TAGCGAGCAC ATCAAATAGT 
TCTCTAAGTG GTCCCAAGAC GCCAGATCCT CATGTCCTGA CAGAGGAGCT AACCCCTAAG 
CGACCTCTGC GTTCGGAAAT GGAGAAGGAA CGTCGACTCT CTCGTCCTGC TAGCATCGCT 
ACGCCAACCG CCAGTATCAA GAATTTCCCT GACACGCGCT CCCTGTCGGA AAGCAGCAAC 
CGCAACTCTG TTGAAACCAC CGATTCCACT TCAGAGGAGG ACATTCGACC TCCGCCTTTG 
CCCGCCAAGG CACGTGACTC AACTGATTTT ACCAGCCTGT CGCAAAACAT GGATTGGACC 
CCGAATGGTT ATGCTATGCT CAGCACTATT AGCAATACCA GCAGCATGAG CACCACCTCA 
ACCCTGACGA AGACCAGTAT TACCAATACC ACGTACGAGT ATTTGGAGAC CACGAACTTC 
AGTTTGGTGG GTGCCATCGA TGGAAACAAA CCGCGTCCGC CGACGCCGCC ACCGAAGCCA 
TCGCGCCACA GCAAACACAT TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mbc cDNA ORF clone, Drosophila melanogaster(fruit fly)

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