Mcm2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcm2
Entrez Gene ID	40973
Full Name	Minichromosome maintenance 2
Synonyms	CG7538,DmMCM2,DmMcm2,DmeMCM2,Dmel\CG7538,MCM2,MCM2-7,MCM2_DROME,McM2,PCR3,dMCM2,dMcm2,l(3)rL074,mcm2-7
General protein information	Preferred Names Minichromosome maintenance 2 Names CG7538 gene product from transcript CG7538-RA CG7538-PA Mcm2-PA minichromosome maintenance 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84F6-84F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_057773.4	NP_477121.1	minichromosome maintenance 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MCM2	XP_516724.2
Macaca mulatta (Rhesus monkey)	MCM2	XP_001099580.2
Rattus norvegicus (Norway rat)	Mcm2	NP_001101343.2
Xenopus tropicalis (tropical clawed frog)	mcm2	NP_001006772.1
Kluyveromyces lactis	KLLA0F12584g	XP_455649.1
Oryza sativa (rice)	Os11g0484300	NP_001067910.1
Canis lupus familiaris (dog)	MCM2	XP_541736.3
Gallus gallus (chicken)	MCM2	NP_001006139.1
Drosophila melanogaster (fruit fly)	Mcm2	NP_477121.1
Arabidopsis thaliana (thale cress)	MCM2	NP_175112.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004275	XP_313198.5
Bos taurus (cattle)	MCM2	XP_002707828.1
Mus musculus (house mouse)	Mcm2	NP_032590.2
Caenorhabditis elegans (roundworm)	mcm-2	NP_001022416.1
Saccharomyces cerevisiae (baker's yeast)	MCM2	NP_009530.1
Magnaporthe oryzae (rice blast fungus)	MGG_08122	XP_003715082.1
Eremothecium gossypii	AGOS_AFR178W	NP_985725.1
Neurospora crassa	NCU04327	XP_960973.1
Homo sapiens (human)	MCM2	NP_004517.2
Danio rerio (zebrafish)	mcm2	NP_775364.1
Schizosaccharomyces pombe (fission yeast)	mcm2	NP_595477.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mcm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08114	NM_057773.4 Latest version!	Drosophila melanogaster minichromosome maintenance 2 (Mcm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcm2 ( NM_057773.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477121.1 Drosophila melanogaster minichromosome maintenance 2 (Mcm2), mRNA.

Price & Availability↑

CloneID	OFa08114
Clone ID Related Accession (Same CDS sequence)	NM_057773.4
Accession Version	NM_057773.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	minichromosome maintenance 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057773.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAATC CCAGTTCACC ACCGCCAAAT ACGCCCAGCG ATGCCGCCGA ACGCCGCGAT 
CTGCGGGCAG CAATGACGTC GCCGGTTGGC GACTTTGAGC CGTTCGAGAA CGAGGACGAG 
ATCCTGGGAG ATCAGACCGT TCGCGATGAG GCCGAGGAGG AGGATGGCGA GGAGCTGTTC 
GGGGACAACA TGGAGAACGA TTACCGGCCC ATGCCTGAGC TGGATCACTA CGATCCGGCG 
CTGCTGGACG ACGAGGATGA CTTCTCGGAG ATGTCCCAGG GCGATCGCTT TGCCGCCGAG 
TCGGAGATGC GTCGACGCGA CCGGGCCGCC GGAATCCACC GCGACGATCG TGATCTGGGT 
TTCGGTCAGT CCGATGATGA GGACGACGTT GGACCCCGGG CCAAGAGACG AGCTGGCGAG 
AAGGCAGCTG TTGGCGAGGT AGAGGACACC GAGATGGTGG AGTCTATTGA AAACCTGGAG 
GATACCAAGG GACACTCAAC TAAAGAGTGG GTCAGTATGC TAGGACCACG CACGGAAATC 
GCCAATCGCT TTCAATCCTT TTTGAGAACT TTTGTGGATG AAAGGGGCGC ATACACCTAT 
CGTGATCGCA TACGTCGGAT GTGCGAGCAG AACATGTCTT CGTTCGTCGT CTCCTACACT 
GATCTGGCCA ACAAGGAACA CGTATTGGCC TATTTCCTGC CCGAGGCTCC CTTTCAAATG 
CTCGAGATTT TCGATAAAGT GGCTAAGGAC ATGGTGCTCT CCATTTTCCC CACTTACGAA 
CGTGTCACCA CAGAGATTCA CGTGCGCATC TCGGAGCTGC CGCTGATCGA GGAGCTGCGC 
ACATTCAGAA AACTGCATCT GAACCAGTTG GTTCGCACTT TGGGCGTGGT AACTGCCACC 
ACCGGAGTTC TTCCCCAGCT ATCCGTGATC AAGTACGACT GCGTTAAGTG CGGCTATGTG 
CTGGGTCCCT TCGTTCAGTC GCAAAATACG GAGATAAAGC CCGGTTCCTG CCCAGAGTGC 
CAGAGCACTG GACCCTTCTC AATCAACATG GAGCAGACGT TGTATCGAAA TTATCAAAAG 
ATCACGTTGC AGGAGTCGCC AGGCAGGATT CCAGCCGGAC GCATTCCTCG CAGCAAGGAT 
GTCATTTTGC TCGCTGATTT GTGTGATCAA TGCAAGCCTG GAGATGAACT TGAGGTCACA 
GGTATATATA CCAATAACTA CGATGGCTCC TTGAACACCG ACCAAGGATT TCCCGTGTTT 
GCCACCGTGA TTATTGCCAA TCATGTGGTT GTAAAGGACT CCAAGCAGGT AGTGCAGTCG 
CTTACGGACG AGGACATTGC CACCATTCAG AAGTTGAGCA AGGATCCGCG TATCGTAGAG 
CGTGTAGTAG CATCCATGGC GCCTTCTATA TACGGACATG ATTATATCAA GAGGGCGCTG 
GCCTTAGCTC TCTTTGGGGG TGAGTCAAAG AATCCCGGTG AGAAACACAA GGTTAGAGGT 
GATATAAACC TACTCATCTG TGGAGATCCC GGAACGGCAA AGTCGCAGTT CCTCAAGTAC 
ACCGAAAAGG TTGCGCCACG TGCGGTTTTC ACGACTGGGC AGGGAGCCAG TGCCGTGGGT 
CTCACGGCTT ATGTGCGCCG CAATCCAGTA TCTCGGGAGT GGACATTGGA AGCGGGTGCT 
CTGGTTCTGG CTGATCAGGG AGTTTGTCTA ATCGACGAGT TCGACAAAAT GAACGACCAG 
GATCGTACCT CTATTCACGA AGCCATGGAG CAGCAGTCTA TTTCGATTTC CAAGGCTGGT 
ATTGTCACCT CTCTTCAAGC TCGTTGCACC GTGATTGCTG CTGCCAATCC CATTGGCGGT 
CGCTATGATC CGTCGATGAC CTTCTCGGAG AACGTGAATC TCTCGGAGCC CATCCTGTCC 
CGTTTCGATG TGCTGTGCGT GGTGAAGGAC GAATTTGACC CCATGCAGGA TCAGCAGCTC 
GCCAAATTCG TGGTACACTC GCATATGAAG CATCACCCGA GTGAGGAGGA GCAGCCGGAG 
TTGGAGGAAC CACAGCTGAA GACCGTTGAT GAGATCCCGC AGGACTTGTT GCGACAATAC 
ATTGTATACG CTAAGGAGAA CATTCGGCCC AAGCTAACGA ACATCGACGA GGACAAGATC 
GCCAAGATGT ACGCCCAGCT GCGACAGGAG TCTTTTGCCA CGGGCTCACT GCCCATTACG 
GTGCGTCACA TTGAGAGTGT CATCCGAATG TCCGAAGCGC ACGCGCGGAT GCATCTGCGC 
GAAAACGTAA TGGAGGCAGA TGTCAGCATG GCCATCCGCA TGATGTTGGA GAGCTTTATC 
GAGGCGCAGA AGTTCAGCGT TATGAAGAAG ATGCGCAGCA CATTCCAGAA GTACCTGTCC 
TTCCAAAAGG ACCATTCCGA GCTGCTGTTC TTTATTCTGC GACAGCTGAC GCTCGACCAG 
CTCGCCTACA TTCGCTGCAA GGACGGGCCT GGCGCCACAC ATGTGGAGAT TATGGAGCGG 
GATTTGATCG AACGGGCTAA GCAGCTGGAT ATTGTAAACC TGAAGCCGTT CTACGAATCA 
GATCTGTTTC GCACAAATGG CTTCTCTTAC GATCCCAAGC GACGCATCAT CCTTCAAATT 
GTGGTCGACG GCAACACAGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477121.1
CDS	132..2795
Translation	MDNPSSPPPNTPSDAAERRDLRAAMTSPVGDFEPFENEDEILGDQTVRDEAEEEDGEELFGDNMENDYRPMPELDHYDPALLDDEDDFSEMSQGDRFAAESEMRRRDRAAGIHRDDRDLGFGQSDDEDDVGPRAKRRAGEKAAVGEVEDTEMVESIENLEDTKGHSTKEWVSMLGPRTEIANRFQSFLRTFVDERGAYTYRDRIRRMCEQNMSSFVVSYTDLANKEHVLAYFLPEAPFQMLEIFDKVAKDMVLSIFPTYERVTTEIHVRISELPLIEELRTFRKLHLNQLVRTLGVVTATTGVLPQLSVIKYDCVKCGYVLGPFVQSQNTEIKPGSCPECQSTGPFSINMEQTLYRNYQKITLQESPGRIPAGRIPRSKDVILLADLCDQCKPGDELEVTGIYTNNYDGSLNTDQGFPVFATVIIANHVVVKDSKQVVQSLTDEDIATIQKLSKDPRIVERVVASMAPSIYGHDYIKRALALALFGGESKNPGEKHKVRGDINLLICGDPGTAKSQFLKYTEKVAPRAVFTTGQGASAVGLTAYVRRNPVSREWTLEAGALVLADQGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSMTFSENVNLSEPILSRFDVLCVVKDEFDPMQDQQLAKFVVHSHMKHHPSEEEQPELEEPQLKTVDEIPQDLLRQYIVYAKENIRPKLTNIDEDKIAKMYAQLRQESFATGSLPITVRHIESVIRMSEAHARMHLRENVMEADVSMAIRMMLESFIEAQKFSVMKKMRSTFQKYLSFQKDHSELLFFILRQLTLDQLAYIRCKDGPGATHVEIMERDLIERAKQLDIVNLKPFYESDLFRTNGFSYDPKRRIILQIVVDGNTA

Target ORF information:

RefSeq Version	NM_057773.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster minichromosome maintenance 2 (Mcm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057773.4

ORF Insert Sequence:

ATGGACAATC CCAGTTCACC ACCGCCAAAT ACGCCCAGCG ATGCCGCCGA ACGCCGCGAT 
CTGCGGGCAG CAATGACGTC GCCGGTTGGC GACTTTGAGC CGTTCGAGAA CGAGGACGAG 
ATCCTGGGAG ATCAGACCGT TCGCGATGAG GCCGAGGAGG AGGATGGCGA GGAGCTGTTC 
GGGGACAACA TGGAGAACGA TTACCGGCCC ATGCCTGAGC TGGATCACTA CGATCCGGCG 
CTGCTGGACG ACGAGGATGA CTTCTCGGAG ATGTCCCAGG GCGATCGCTT TGCCGCCGAG 
TCGGAGATGC GTCGACGCGA CCGGGCCGCC GGAATCCACC GCGACGATCG TGATCTGGGT 
TTCGGTCAGT CCGATGATGA GGACGACGTT GGACCCCGGG CCAAGAGACG AGCTGGCGAG 
AAGGCAGCTG TTGGCGAGGT AGAGGACACC GAGATGGTGG AGTCTATTGA AAACCTGGAG 
GATACCAAGG GACACTCAAC TAAAGAGTGG GTCAGTATGC TAGGACCACG CACGGAAATC 
GCCAATCGCT TTCAATCCTT TTTGAGAACT TTTGTGGATG AAAGGGGCGC ATACACCTAT 
CGTGATCGCA TACGTCGGAT GTGCGAGCAG AACATGTCTT CGTTCGTCGT CTCCTACACT 
GATCTGGCCA ACAAGGAACA CGTATTGGCC TATTTCCTGC CCGAGGCTCC CTTTCAAATG 
CTCGAGATTT TCGATAAAGT GGCTAAGGAC ATGGTGCTCT CCATTTTCCC CACTTACGAA 
CGTGTCACCA CAGAGATTCA CGTGCGCATC TCGGAGCTGC CGCTGATCGA GGAGCTGCGC 
ACATTCAGAA AACTGCATCT GAACCAGTTG GTTCGCACTT TGGGCGTGGT AACTGCCACC 
ACCGGAGTTC TTCCCCAGCT ATCCGTGATC AAGTACGACT GCGTTAAGTG CGGCTATGTG 
CTGGGTCCCT TCGTTCAGTC GCAAAATACG GAGATAAAGC CCGGTTCCTG CCCAGAGTGC 
CAGAGCACTG GACCCTTCTC AATCAACATG GAGCAGACGT TGTATCGAAA TTATCAAAAG 
ATCACGTTGC AGGAGTCGCC AGGCAGGATT CCAGCCGGAC GCATTCCTCG CAGCAAGGAT 
GTCATTTTGC TCGCTGATTT GTGTGATCAA TGCAAGCCTG GAGATGAACT TGAGGTCACA 
GGTATATATA CCAATAACTA CGATGGCTCC TTGAACACCG ACCAAGGATT TCCCGTGTTT 
GCCACCGTGA TTATTGCCAA TCATGTGGTT GTAAAGGACT CCAAGCAGGT AGTGCAGTCG 
CTTACGGACG AGGACATTGC CACCATTCAG AAGTTGAGCA AGGATCCGCG TATCGTAGAG 
CGTGTAGTAG CATCCATGGC GCCTTCTATA TACGGACATG ATTATATCAA GAGGGCGCTG 
GCCTTAGCTC TCTTTGGGGG TGAGTCAAAG AATCCCGGTG AGAAACACAA GGTTAGAGGT 
GATATAAACC TACTCATCTG TGGAGATCCC GGAACGGCAA AGTCGCAGTT CCTCAAGTAC 
ACCGAAAAGG TTGCGCCACG TGCGGTTTTC ACGACTGGGC AGGGAGCCAG TGCCGTGGGT 
CTCACGGCTT ATGTGCGCCG CAATCCAGTA TCTCGGGAGT GGACATTGGA AGCGGGTGCT 
CTGGTTCTGG CTGATCAGGG AGTTTGTCTA ATCGACGAGT TCGACAAAAT GAACGACCAG 
GATCGTACCT CTATTCACGA AGCCATGGAG CAGCAGTCTA TTTCGATTTC CAAGGCTGGT 
ATTGTCACCT CTCTTCAAGC TCGTTGCACC GTGATTGCTG CTGCCAATCC CATTGGCGGT 
CGCTATGATC CGTCGATGAC CTTCTCGGAG AACGTGAATC TCTCGGAGCC CATCCTGTCC 
CGTTTCGATG TGCTGTGCGT GGTGAAGGAC GAATTTGACC CCATGCAGGA TCAGCAGCTC 
GCCAAATTCG TGGTACACTC GCATATGAAG CATCACCCGA GTGAGGAGGA GCAGCCGGAG 
TTGGAGGAAC CACAGCTGAA GACCGTTGAT GAGATCCCGC AGGACTTGTT GCGACAATAC 
ATTGTATACG CTAAGGAGAA CATTCGGCCC AAGCTAACGA ACATCGACGA GGACAAGATC 
GCCAAGATGT ACGCCCAGCT GCGACAGGAG TCTTTTGCCA CGGGCTCACT GCCCATTACG 
GTGCGTCACA TTGAGAGTGT CATCCGAATG TCCGAAGCGC ACGCGCGGAT GCATCTGCGC 
GAAAACGTAA TGGAGGCAGA TGTCAGCATG GCCATCCGCA TGATGTTGGA GAGCTTTATC 
GAGGCGCAGA AGTTCAGCGT TATGAAGAAG ATGCGCAGCA CATTCCAGAA GTACCTGTCC 
TTCCAAAAGG ACCATTCCGA GCTGCTGTTC TTTATTCTGC GACAGCTGAC GCTCGACCAG 
CTCGCCTACA TTCGCTGCAA GGACGGGCCT GGCGCCACAC ATGTGGAGAT TATGGAGCGG 
GATTTGATCG AACGGGCTAA GCAGCTGGAT ATTGTAAACC TGAAGCCGTT CTACGAATCA 
GATCTGTTTC GCACAAATGG CTTCTCTTAC GATCCCAAGC GACGCATCAT CCTTCAAATT 
GTGGTCGACG GCAACACAGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mcm2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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