SdhA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	SdhA
Entrez Gene ID	37228
Full Name	Succinate dehydrogenase, subunit A (flavoprotein)
Synonyms	CG17246,DHSA,Dmel\CG17246,SDH,Scs-fp,Sdh,Sdh A,Sdha,scs-fp,sdh
General protein information	Preferred Names Succinate dehydrogenase, subunit A (flavoprotein) Names CG17246 gene product from transcript CG17246-RB CG17246-PA CG17246-PB CG17246-PC COMPLEX II: flavoprotein subunit SUCCINYL COENZIME A SYNTHETASE FLAVOPROTEIN SUBUNIT SdhA-PA SdhA-PB SdhA-PC succinate dehydrogenase succinate dehydrogenase A succinate dehydrogenase [ubiquinone] flavoprotein subunit succinate dehydrogenase flavoprotein succinate dehydrogenase flavoprotein subunit succinate dehydrogenase, subunit A (flavoprotein) succinyl CoA synthetase flavoprotein subunit of complex II succinyl coenzyme A synthetase flavoprotein subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56D3-56D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_166344.3	NP_725882.1	succinate dehydrogenase, subunit A (flavoprotein), isoform C
NM_057862.5	NP_477210.1	succinate dehydrogenase, subunit A (flavoprotein), isoform A
NM_166343.3	NP_725881.1	succinate dehydrogenase, subunit A (flavoprotein), isoform B

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os07g0134800	NP_001058845.1
Schizosaccharomyces pombe (fission yeast)	sdh1	NP_594319.1
Eremothecium gossypii	AGOS_ACR052W	NP_983455.2
Gallus gallus (chicken)	SDHA	NP_001264327.1
Drosophila melanogaster (fruit fly)	SdhA	NP_725882.1
Bos taurus (cattle)	SDHA	NP_776603.1
Mus musculus (house mouse)	Sdha	NP_075770.1
Rattus norvegicus (Norway rat)	Sdha	NP_569112.1
Danio rerio (zebrafish)	sdha	NP_957204.1
Caenorhabditis elegans (roundworm)	sdha-2	NP_492798.1
Arabidopsis thaliana (thale cress)	SDH1-1	NP_201477.1
Kluyveromyces lactis	KLLA0D04444g	XP_453260.1
Saccharomyces cerevisiae (baker's yeast)	SDH1	NP_012774.1
Neurospora crassa	NCU08336	XP_001728186.1
Macaca mulatta (Rhesus monkey)	LOC708018	XP_001094170.2
Canis lupus familiaris (dog)	SDHA	XP_535807.4
Xenopus tropicalis (tropical clawed frog)	sdha	NP_001015989.1
Magnaporthe oryzae (rice blast fungus)	MGG_00168	XP_003718957.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010429	XP_311518.4
Pan troglodytes (chimpanzee)	SDHA	XP_003310656.1
Homo sapiens (human)	SDHA	NP_004159.2
Caenorhabditis elegans (roundworm)	sdha-1	NP_509446.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SdhA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SdhA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10626	NM_057862.5 Latest version!	Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant A (SdhA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa10626	NM_166343.3 Latest version!	Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant B (SdhA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa10626	NM_166344.3 Latest version!	Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant C (SdhA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SdhA ( NM_166344.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725882.1 Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant C (SdhA), mRNA.

Price & Availability↑

CloneID	OFa10626
Clone ID Related Accession (Same CDS sequence)	NM_166344.3 , NM_057862.5 , NM_166343.3
Accession Version	NM_166344.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	succinate dehydrogenase, subunit A (flavoprotein), isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166344.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGGAA TCATGCGTGT GCCATCGATT TTGGCCAAAA ATGCAGTCGC CTCCATGCAA 
CGTGCCGCAG CCGTTGGAGT GCAGCGCAGT TACCACATCA CACACGGCCG CCAGCAGGCC 
TCGGCGGCGA ATCCGGACAA GATATCGAAG CAGTACCCGG TGGTGGATCA CGCCTACGAT 
GCGATCGTCG TGGGAGCAGG AGGAGCCGGC TTGCGGGCGG CTTTTGGCCT GGTGGCTGAG 
GGATTTCGCA CGGCGGTGAT CACCAAGCTT TTCCCCACCC GCTCGCACAC GATCGCCGCC 
CAGGGCGGTA TCAATGCCGC TCTCGGCAAC ATGGAGGAGG ACGACTGGAA GTGGCACATG 
TACGACACGG TCAAGGGCTC CGATTGGCTG GGCGACCAGG ATGCCATCCA CTACATGACC 
CGCGAGGCGC CCAAGGCTGT CATTGAGCTG GAGAACTACG GCATGCCCTT CTCGCGTACC 
CAGGACGGTA AGATCTACCA GCGCGCCTTC GGTGGACAGA GTCTGAAGTT CGGCAAGGGC 
GGACAGGCCC ATCGCTGCTG TGCTGTGGCT GATCGTACTG GTCACTCGCT GCTGCACACG 
CTATACGGTC AATCGCTGAG CTACGACTGC AACTACTTTG TGGAGTACTT TGCCCTGGAT 
CTGATCTTCG AGGACGGCGA GTGCCGTGGT GTGCTGGCCC TGAACCTGGA GGATGGCACA 
CTGCACCGAT TCCGCGCTAA GAACACGGTC ATTGCCACCG GTGGATATGG ACGAGCATTC 
TTCTCCTGCA CCTCGGCGCA CACATGTACC GGTGACGGTA CTGCTATGGT TGCACGCCAG 
GGACTGCCCT CTCAGGATCT GGAGTTCGTG CAGTTCCATC CTACTGGCAT CTACGGCGCC 
GGATGTCTCA TCACCGAGGG CTGCCGCGGT GAGGGTGGTT ACCTGATCAA CGGTAATGGT 
GAGCGCTTCA TGGAGCGCTA TGCTCCTGTG GCCAAGGATC TGGCCTCTCG CGACGTCGTC 
TCGCGGTCGA TGACCATCGA GATCATGGAG GGTCGTGGCG CTGGACCCGA GAAGGATCAC 
GTGTACCTGC AACTGCACCA CTTGCCGCCC AAGCAGCTCG CGGAGCGTCT GCCTGGCATC 
TCCGAGACCG CCATGATCTT CGCCGGTGTT GATGTGACCC GTGAGCCCAT CCCCGTGTTG 
CCCACCGTGC ATTACAACAT GGGCGGTGTG CCGACTAACT ATCGCGGCCA GGTAATCACC 
ATTGACAAGG ATGGCAAGGA TGTGATTGTG CCGGGACTGT ATGCCGCTGG TGAGGCTGCT 
TCCAGCTCGG TGCATGGTGC CAACCGTCTG GGTGCCAACT CTCTGCTGGA TCTGGTGGTC 
TTCGGACGTG CATGCGCCAA GACCATCGCC GAGCTGAATA AGCCTGGTGC ACCGGCTCCC 
ACCCTCAAGG AAAACGCTGG CGAGGCCTCT GTTGCTAACC TCGATAAGCT GCGCCATGCC 
AACGGCCAGA TCACCACCGC CGATCTGCGT CTGAAGATGC AGAAGACCAT GCAGCATCAT 
GCTGCTGTGT TCCGCGATGG CCCCATCCTG CAGGACGGTG TGAACAAGAT GAAGGAGATC 
TACAAGCAGT TCAAGGACAT CAAGGTTGTC GATAGGTCGC TTATCTGGAA CTCCGATCTG 
GTGGAGACGC TGGAGCTGCA GAATCTGTTG GCCAATGCCC AGATGACTAT TGTGAGCGCT 
GAGGCGCGCA AGGAATCGCG AGGTGCCCAC GCCCGCGAGG ACTTCAAGGT TCGCGAGGAT 
GAATACGATT TCAGCAAGCC CCTGGATGGT CAGCAGAAGA AGCCCATGGA TCAGCACTGG 
CGCAAGCACA CGCTCTCGTG GGTGTGCAAT GACAACGGAG ACATTACGCT GGACTACAGA 
AACGTGATCG ATACCACGCT GGACAACGAA GTCTCTACTG TTCCACCAGC GATTCGCTCC 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725882.1
CDS	142..2127
Translation	MSGIMRVPSILAKNAVASMQRAAAVGVQRSYHITHGRQQASAANPDKISKQYPVVDHAYDAIVVGAGGAGLRAAFGLVAEGFRTAVITKLFPTRSHTIAAQGGINAALGNMEEDDWKWHMYDTVKGSDWLGDQDAIHYMTREAPKAVIELENYGMPFSRTQDGKIYQRAFGGQSLKFGKGGQAHRCCAVADRTGHSLLHTLYGQSLSYDCNYFVEYFALDLIFEDGECRGVLALNLEDGTLHRFRAKNTVIATGGYGRAFFSCTSAHTCTGDGTAMVARQGLPSQDLEFVQFHPTGIYGAGCLITEGCRGEGGYLINGNGERFMERYAPVAKDLASRDVVSRSMTIEIMEGRGAGPEKDHVYLQLHHLPPKQLAERLPGISETAMIFAGVDVTREPIPVLPTVHYNMGGVPTNYRGQVITIDKDGKDVIVPGLYAAGEAASSSVHGANRLGANSLLDLVVFGRACAKTIAELNKPGAPAPTLKENAGEASVANLDKLRHANGQITTADLRLKMQKTMQHHAAVFRDGPILQDGVNKMKEIYKQFKDIKVVDRSLIWNSDLVETLELQNLLANAQMTIVSAEARKESRGAHAREDFKVREDEYDFSKPLDGQQKKPMDQHWRKHTLSWVCNDNGDITLDYRNVIDTTLDNEVSTVPPAIRSY

Target ORF information:

RefSeq Version	NM_166344.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant C (SdhA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166344.3

ORF Insert Sequence:

ATGTCCGGAA TCATGCGTGT GCCATCGATT TTGGCCAAAA ATGCAGTCGC CTCCATGCAA 
CGTGCCGCAG CCGTTGGAGT GCAGCGCAGT TACCACATCA CACACGGCCG CCAGCAGGCC 
TCGGCGGCGA ATCCGGACAA GATATCGAAG CAGTACCCGG TGGTGGATCA CGCCTACGAT 
GCGATCGTCG TGGGAGCAGG AGGAGCCGGC TTGCGGGCGG CTTTTGGCCT GGTGGCTGAG 
GGATTTCGCA CGGCGGTGAT CACCAAGCTT TTCCCCACCC GCTCGCACAC GATCGCCGCC 
CAGGGCGGTA TCAATGCCGC TCTCGGCAAC ATGGAGGAGG ACGACTGGAA GTGGCACATG 
TACGACACGG TCAAGGGCTC CGATTGGCTG GGCGACCAGG ATGCCATCCA CTACATGACC 
CGCGAGGCGC CCAAGGCTGT CATTGAGCTG GAGAACTACG GCATGCCCTT CTCGCGTACC 
CAGGACGGTA AGATCTACCA GCGCGCCTTC GGTGGACAGA GTCTGAAGTT CGGCAAGGGC 
GGACAGGCCC ATCGCTGCTG TGCTGTGGCT GATCGTACTG GTCACTCGCT GCTGCACACG 
CTATACGGTC AATCGCTGAG CTACGACTGC AACTACTTTG TGGAGTACTT TGCCCTGGAT 
CTGATCTTCG AGGACGGCGA GTGCCGTGGT GTGCTGGCCC TGAACCTGGA GGATGGCACA 
CTGCACCGAT TCCGCGCTAA GAACACGGTC ATTGCCACCG GTGGATATGG ACGAGCATTC 
TTCTCCTGCA CCTCGGCGCA CACATGTACC GGTGACGGTA CTGCTATGGT TGCACGCCAG 
GGACTGCCCT CTCAGGATCT GGAGTTCGTG CAGTTCCATC CTACTGGCAT CTACGGCGCC 
GGATGTCTCA TCACCGAGGG CTGCCGCGGT GAGGGTGGTT ACCTGATCAA CGGTAATGGT 
GAGCGCTTCA TGGAGCGCTA TGCTCCTGTG GCCAAGGATC TGGCCTCTCG CGACGTCGTC 
TCGCGGTCGA TGACCATCGA GATCATGGAG GGTCGTGGCG CTGGACCCGA GAAGGATCAC 
GTGTACCTGC AACTGCACCA CTTGCCGCCC AAGCAGCTCG CGGAGCGTCT GCCTGGCATC 
TCCGAGACCG CCATGATCTT CGCCGGTGTT GATGTGACCC GTGAGCCCAT CCCCGTGTTG 
CCCACCGTGC ATTACAACAT GGGCGGTGTG CCGACTAACT ATCGCGGCCA GGTAATCACC 
ATTGACAAGG ATGGCAAGGA TGTGATTGTG CCGGGACTGT ATGCCGCTGG TGAGGCTGCT 
TCCAGCTCGG TGCATGGTGC CAACCGTCTG GGTGCCAACT CTCTGCTGGA TCTGGTGGTC 
TTCGGACGTG CATGCGCCAA GACCATCGCC GAGCTGAATA AGCCTGGTGC ACCGGCTCCC 
ACCCTCAAGG AAAACGCTGG CGAGGCCTCT GTTGCTAACC TCGATAAGCT GCGCCATGCC 
AACGGCCAGA TCACCACCGC CGATCTGCGT CTGAAGATGC AGAAGACCAT GCAGCATCAT 
GCTGCTGTGT TCCGCGATGG CCCCATCCTG CAGGACGGTG TGAACAAGAT GAAGGAGATC 
TACAAGCAGT TCAAGGACAT CAAGGTTGTC GATAGGTCGC TTATCTGGAA CTCCGATCTG 
GTGGAGACGC TGGAGCTGCA GAATCTGTTG GCCAATGCCC AGATGACTAT TGTGAGCGCT 
GAGGCGCGCA AGGAATCGCG AGGTGCCCAC GCCCGCGAGG ACTTCAAGGT TCGCGAGGAT 
GAATACGATT TCAGCAAGCC CCTGGATGGT CAGCAGAAGA AGCCCATGGA TCAGCACTGG 
CGCAAGCACA CGCTCTCGTG GGTGTGCAAT GACAACGGAG ACATTACGCT GGACTACAGA 
AACGTGATCG ATACCACGCT GGACAACGAA GTCTCTACTG TTCCACCAGC GATTCGCTCC 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SdhA ( NM_057862.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477210.1 Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant A (SdhA), mRNA.

Price & Availability↑

CloneID	OFa10626
Clone ID Related Accession (Same CDS sequence)	NM_166344.3 , NM_057862.5 , NM_166343.3
Accession Version	NM_057862.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	succinate dehydrogenase, subunit A (flavoprotein), isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057862.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGGAA TCATGCGTGT GCCATCGATT TTGGCCAAAA ATGCAGTCGC CTCCATGCAA 
CGTGCCGCAG CCGTTGGAGT GCAGCGCAGT TACCACATCA CACACGGCCG CCAGCAGGCC 
TCGGCGGCGA ATCCGGACAA GATATCGAAG CAGTACCCGG TGGTGGATCA CGCCTACGAT 
GCGATCGTCG TGGGAGCAGG AGGAGCCGGC TTGCGGGCGG CTTTTGGCCT GGTGGCTGAG 
GGATTTCGCA CGGCGGTGAT CACCAAGCTT TTCCCCACCC GCTCGCACAC GATCGCCGCC 
CAGGGCGGTA TCAATGCCGC TCTCGGCAAC ATGGAGGAGG ACGACTGGAA GTGGCACATG 
TACGACACGG TCAAGGGCTC CGATTGGCTG GGCGACCAGG ATGCCATCCA CTACATGACC 
CGCGAGGCGC CCAAGGCTGT CATTGAGCTG GAGAACTACG GCATGCCCTT CTCGCGTACC 
CAGGACGGTA AGATCTACCA GCGCGCCTTC GGTGGACAGA GTCTGAAGTT CGGCAAGGGC 
GGACAGGCCC ATCGCTGCTG TGCTGTGGCT GATCGTACTG GTCACTCGCT GCTGCACACG 
CTATACGGTC AATCGCTGAG CTACGACTGC AACTACTTTG TGGAGTACTT TGCCCTGGAT 
CTGATCTTCG AGGACGGCGA GTGCCGTGGT GTGCTGGCCC TGAACCTGGA GGATGGCACA 
CTGCACCGAT TCCGCGCTAA GAACACGGTC ATTGCCACCG GTGGATATGG ACGAGCATTC 
TTCTCCTGCA CCTCGGCGCA CACATGTACC GGTGACGGTA CTGCTATGGT TGCACGCCAG 
GGACTGCCCT CTCAGGATCT GGAGTTCGTG CAGTTCCATC CTACTGGCAT CTACGGCGCC 
GGATGTCTCA TCACCGAGGG CTGCCGCGGT GAGGGTGGTT ACCTGATCAA CGGTAATGGT 
GAGCGCTTCA TGGAGCGCTA TGCTCCTGTG GCCAAGGATC TGGCCTCTCG CGACGTCGTC 
TCGCGGTCGA TGACCATCGA GATCATGGAG GGTCGTGGCG CTGGACCCGA GAAGGATCAC 
GTGTACCTGC AACTGCACCA CTTGCCGCCC AAGCAGCTCG CGGAGCGTCT GCCTGGCATC 
TCCGAGACCG CCATGATCTT CGCCGGTGTT GATGTGACCC GTGAGCCCAT CCCCGTGTTG 
CCCACCGTGC ATTACAACAT GGGCGGTGTG CCGACTAACT ATCGCGGCCA GGTAATCACC 
ATTGACAAGG ATGGCAAGGA TGTGATTGTG CCGGGACTGT ATGCCGCTGG TGAGGCTGCT 
TCCAGCTCGG TGCATGGTGC CAACCGTCTG GGTGCCAACT CTCTGCTGGA TCTGGTGGTC 
TTCGGACGTG CATGCGCCAA GACCATCGCC GAGCTGAATA AGCCTGGTGC ACCGGCTCCC 
ACCCTCAAGG AAAACGCTGG CGAGGCCTCT GTTGCTAACC TCGATAAGCT GCGCCATGCC 
AACGGCCAGA TCACCACCGC CGATCTGCGT CTGAAGATGC AGAAGACCAT GCAGCATCAT 
GCTGCTGTGT TCCGCGATGG CCCCATCCTG CAGGACGGTG TGAACAAGAT GAAGGAGATC 
TACAAGCAGT TCAAGGACAT CAAGGTTGTC GATAGGTCGC TTATCTGGAA CTCCGATCTG 
GTGGAGACGC TGGAGCTGCA GAATCTGTTG GCCAATGCCC AGATGACTAT TGTGAGCGCT 
GAGGCGCGCA AGGAATCGCG AGGTGCCCAC GCCCGCGAGG ACTTCAAGGT TCGCGAGGAT 
GAATACGATT TCAGCAAGCC CCTGGATGGT CAGCAGAAGA AGCCCATGGA TCAGCACTGG 
CGCAAGCACA CGCTCTCGTG GGTGTGCAAT GACAACGGAG ACATTACGCT GGACTACAGA 
AACGTGATCG ATACCACGCT GGACAACGAA GTCTCTACTG TTCCACCAGC GATTCGCTCC 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477210.1
CDS	229..2214
Translation	MSGIMRVPSILAKNAVASMQRAAAVGVQRSYHITHGRQQASAANPDKISKQYPVVDHAYDAIVVGAGGAGLRAAFGLVAEGFRTAVITKLFPTRSHTIAAQGGINAALGNMEEDDWKWHMYDTVKGSDWLGDQDAIHYMTREAPKAVIELENYGMPFSRTQDGKIYQRAFGGQSLKFGKGGQAHRCCAVADRTGHSLLHTLYGQSLSYDCNYFVEYFALDLIFEDGECRGVLALNLEDGTLHRFRAKNTVIATGGYGRAFFSCTSAHTCTGDGTAMVARQGLPSQDLEFVQFHPTGIYGAGCLITEGCRGEGGYLINGNGERFMERYAPVAKDLASRDVVSRSMTIEIMEGRGAGPEKDHVYLQLHHLPPKQLAERLPGISETAMIFAGVDVTREPIPVLPTVHYNMGGVPTNYRGQVITIDKDGKDVIVPGLYAAGEAASSSVHGANRLGANSLLDLVVFGRACAKTIAELNKPGAPAPTLKENAGEASVANLDKLRHANGQITTADLRLKMQKTMQHHAAVFRDGPILQDGVNKMKEIYKQFKDIKVVDRSLIWNSDLVETLELQNLLANAQMTIVSAEARKESRGAHAREDFKVREDEYDFSKPLDGQQKKPMDQHWRKHTLSWVCNDNGDITLDYRNVIDTTLDNEVSTVPPAIRSY

Target ORF information:

RefSeq Version	NM_057862.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant A (SdhA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057862.5

ORF Insert Sequence:

ATGTCCGGAA TCATGCGTGT GCCATCGATT TTGGCCAAAA ATGCAGTCGC CTCCATGCAA 
CGTGCCGCAG CCGTTGGAGT GCAGCGCAGT TACCACATCA CACACGGCCG CCAGCAGGCC 
TCGGCGGCGA ATCCGGACAA GATATCGAAG CAGTACCCGG TGGTGGATCA CGCCTACGAT 
GCGATCGTCG TGGGAGCAGG AGGAGCCGGC TTGCGGGCGG CTTTTGGCCT GGTGGCTGAG 
GGATTTCGCA CGGCGGTGAT CACCAAGCTT TTCCCCACCC GCTCGCACAC GATCGCCGCC 
CAGGGCGGTA TCAATGCCGC TCTCGGCAAC ATGGAGGAGG ACGACTGGAA GTGGCACATG 
TACGACACGG TCAAGGGCTC CGATTGGCTG GGCGACCAGG ATGCCATCCA CTACATGACC 
CGCGAGGCGC CCAAGGCTGT CATTGAGCTG GAGAACTACG GCATGCCCTT CTCGCGTACC 
CAGGACGGTA AGATCTACCA GCGCGCCTTC GGTGGACAGA GTCTGAAGTT CGGCAAGGGC 
GGACAGGCCC ATCGCTGCTG TGCTGTGGCT GATCGTACTG GTCACTCGCT GCTGCACACG 
CTATACGGTC AATCGCTGAG CTACGACTGC AACTACTTTG TGGAGTACTT TGCCCTGGAT 
CTGATCTTCG AGGACGGCGA GTGCCGTGGT GTGCTGGCCC TGAACCTGGA GGATGGCACA 
CTGCACCGAT TCCGCGCTAA GAACACGGTC ATTGCCACCG GTGGATATGG ACGAGCATTC 
TTCTCCTGCA CCTCGGCGCA CACATGTACC GGTGACGGTA CTGCTATGGT TGCACGCCAG 
GGACTGCCCT CTCAGGATCT GGAGTTCGTG CAGTTCCATC CTACTGGCAT CTACGGCGCC 
GGATGTCTCA TCACCGAGGG CTGCCGCGGT GAGGGTGGTT ACCTGATCAA CGGTAATGGT 
GAGCGCTTCA TGGAGCGCTA TGCTCCTGTG GCCAAGGATC TGGCCTCTCG CGACGTCGTC 
TCGCGGTCGA TGACCATCGA GATCATGGAG GGTCGTGGCG CTGGACCCGA GAAGGATCAC 
GTGTACCTGC AACTGCACCA CTTGCCGCCC AAGCAGCTCG CGGAGCGTCT GCCTGGCATC 
TCCGAGACCG CCATGATCTT CGCCGGTGTT GATGTGACCC GTGAGCCCAT CCCCGTGTTG 
CCCACCGTGC ATTACAACAT GGGCGGTGTG CCGACTAACT ATCGCGGCCA GGTAATCACC 
ATTGACAAGG ATGGCAAGGA TGTGATTGTG CCGGGACTGT ATGCCGCTGG TGAGGCTGCT 
TCCAGCTCGG TGCATGGTGC CAACCGTCTG GGTGCCAACT CTCTGCTGGA TCTGGTGGTC 
TTCGGACGTG CATGCGCCAA GACCATCGCC GAGCTGAATA AGCCTGGTGC ACCGGCTCCC 
ACCCTCAAGG AAAACGCTGG CGAGGCCTCT GTTGCTAACC TCGATAAGCT GCGCCATGCC 
AACGGCCAGA TCACCACCGC CGATCTGCGT CTGAAGATGC AGAAGACCAT GCAGCATCAT 
GCTGCTGTGT TCCGCGATGG CCCCATCCTG CAGGACGGTG TGAACAAGAT GAAGGAGATC 
TACAAGCAGT TCAAGGACAT CAAGGTTGTC GATAGGTCGC TTATCTGGAA CTCCGATCTG 
GTGGAGACGC TGGAGCTGCA GAATCTGTTG GCCAATGCCC AGATGACTAT TGTGAGCGCT 
GAGGCGCGCA AGGAATCGCG AGGTGCCCAC GCCCGCGAGG ACTTCAAGGT TCGCGAGGAT 
GAATACGATT TCAGCAAGCC CCTGGATGGT CAGCAGAAGA AGCCCATGGA TCAGCACTGG 
CGCAAGCACA CGCTCTCGTG GGTGTGCAAT GACAACGGAG ACATTACGCT GGACTACAGA 
AACGTGATCG ATACCACGCT GGACAACGAA GTCTCTACTG TTCCACCAGC GATTCGCTCC 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SdhA ( NM_166343.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725881.1 Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant B (SdhA), mRNA.

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CloneID	OFa10626
Clone ID Related Accession (Same CDS sequence)	NM_166344.3 , NM_057862.5 , NM_166343.3
Accession Version	NM_166343.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	succinate dehydrogenase, subunit A (flavoprotein), isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166343.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGGAA TCATGCGTGT GCCATCGATT TTGGCCAAAA ATGCAGTCGC CTCCATGCAA 
CGTGCCGCAG CCGTTGGAGT GCAGCGCAGT TACCACATCA CACACGGCCG CCAGCAGGCC 
TCGGCGGCGA ATCCGGACAA GATATCGAAG CAGTACCCGG TGGTGGATCA CGCCTACGAT 
GCGATCGTCG TGGGAGCAGG AGGAGCCGGC TTGCGGGCGG CTTTTGGCCT GGTGGCTGAG 
GGATTTCGCA CGGCGGTGAT CACCAAGCTT TTCCCCACCC GCTCGCACAC GATCGCCGCC 
CAGGGCGGTA TCAATGCCGC TCTCGGCAAC ATGGAGGAGG ACGACTGGAA GTGGCACATG 
TACGACACGG TCAAGGGCTC CGATTGGCTG GGCGACCAGG ATGCCATCCA CTACATGACC 
CGCGAGGCGC CCAAGGCTGT CATTGAGCTG GAGAACTACG GCATGCCCTT CTCGCGTACC 
CAGGACGGTA AGATCTACCA GCGCGCCTTC GGTGGACAGA GTCTGAAGTT CGGCAAGGGC 
GGACAGGCCC ATCGCTGCTG TGCTGTGGCT GATCGTACTG GTCACTCGCT GCTGCACACG 
CTATACGGTC AATCGCTGAG CTACGACTGC AACTACTTTG TGGAGTACTT TGCCCTGGAT 
CTGATCTTCG AGGACGGCGA GTGCCGTGGT GTGCTGGCCC TGAACCTGGA GGATGGCACA 
CTGCACCGAT TCCGCGCTAA GAACACGGTC ATTGCCACCG GTGGATATGG ACGAGCATTC 
TTCTCCTGCA CCTCGGCGCA CACATGTACC GGTGACGGTA CTGCTATGGT TGCACGCCAG 
GGACTGCCCT CTCAGGATCT GGAGTTCGTG CAGTTCCATC CTACTGGCAT CTACGGCGCC 
GGATGTCTCA TCACCGAGGG CTGCCGCGGT GAGGGTGGTT ACCTGATCAA CGGTAATGGT 
GAGCGCTTCA TGGAGCGCTA TGCTCCTGTG GCCAAGGATC TGGCCTCTCG CGACGTCGTC 
TCGCGGTCGA TGACCATCGA GATCATGGAG GGTCGTGGCG CTGGACCCGA GAAGGATCAC 
GTGTACCTGC AACTGCACCA CTTGCCGCCC AAGCAGCTCG CGGAGCGTCT GCCTGGCATC 
TCCGAGACCG CCATGATCTT CGCCGGTGTT GATGTGACCC GTGAGCCCAT CCCCGTGTTG 
CCCACCGTGC ATTACAACAT GGGCGGTGTG CCGACTAACT ATCGCGGCCA GGTAATCACC 
ATTGACAAGG ATGGCAAGGA TGTGATTGTG CCGGGACTGT ATGCCGCTGG TGAGGCTGCT 
TCCAGCTCGG TGCATGGTGC CAACCGTCTG GGTGCCAACT CTCTGCTGGA TCTGGTGGTC 
TTCGGACGTG CATGCGCCAA GACCATCGCC GAGCTGAATA AGCCTGGTGC ACCGGCTCCC 
ACCCTCAAGG AAAACGCTGG CGAGGCCTCT GTTGCTAACC TCGATAAGCT GCGCCATGCC 
AACGGCCAGA TCACCACCGC CGATCTGCGT CTGAAGATGC AGAAGACCAT GCAGCATCAT 
GCTGCTGTGT TCCGCGATGG CCCCATCCTG CAGGACGGTG TGAACAAGAT GAAGGAGATC 
TACAAGCAGT TCAAGGACAT CAAGGTTGTC GATAGGTCGC TTATCTGGAA CTCCGATCTG 
GTGGAGACGC TGGAGCTGCA GAATCTGTTG GCCAATGCCC AGATGACTAT TGTGAGCGCT 
GAGGCGCGCA AGGAATCGCG AGGTGCCCAC GCCCGCGAGG ACTTCAAGGT TCGCGAGGAT 
GAATACGATT TCAGCAAGCC CCTGGATGGT CAGCAGAAGA AGCCCATGGA TCAGCACTGG 
CGCAAGCACA CGCTCTCGTG GGTGTGCAAT GACAACGGAG ACATTACGCT GGACTACAGA 
AACGTGATCG ATACCACGCT GGACAACGAA GTCTCTACTG TTCCACCAGC GATTCGCTCC 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725881.1
CDS	96..2081
Translation	MSGIMRVPSILAKNAVASMQRAAAVGVQRSYHITHGRQQASAANPDKISKQYPVVDHAYDAIVVGAGGAGLRAAFGLVAEGFRTAVITKLFPTRSHTIAAQGGINAALGNMEEDDWKWHMYDTVKGSDWLGDQDAIHYMTREAPKAVIELENYGMPFSRTQDGKIYQRAFGGQSLKFGKGGQAHRCCAVADRTGHSLLHTLYGQSLSYDCNYFVEYFALDLIFEDGECRGVLALNLEDGTLHRFRAKNTVIATGGYGRAFFSCTSAHTCTGDGTAMVARQGLPSQDLEFVQFHPTGIYGAGCLITEGCRGEGGYLINGNGERFMERYAPVAKDLASRDVVSRSMTIEIMEGRGAGPEKDHVYLQLHHLPPKQLAERLPGISETAMIFAGVDVTREPIPVLPTVHYNMGGVPTNYRGQVITIDKDGKDVIVPGLYAAGEAASSSVHGANRLGANSLLDLVVFGRACAKTIAELNKPGAPAPTLKENAGEASVANLDKLRHANGQITTADLRLKMQKTMQHHAAVFRDGPILQDGVNKMKEIYKQFKDIKVVDRSLIWNSDLVETLELQNLLANAQMTIVSAEARKESRGAHAREDFKVREDEYDFSKPLDGQQKKPMDQHWRKHTLSWVCNDNGDITLDYRNVIDTTLDNEVSTVPPAIRSY

Target ORF information:

RefSeq Version	NM_166343.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster succinate dehydrogenase, subunit A (flavoprotein), transcript variant B (SdhA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166343.3

ORF Insert Sequence:

ATGTCCGGAA TCATGCGTGT GCCATCGATT TTGGCCAAAA ATGCAGTCGC CTCCATGCAA 
CGTGCCGCAG CCGTTGGAGT GCAGCGCAGT TACCACATCA CACACGGCCG CCAGCAGGCC 
TCGGCGGCGA ATCCGGACAA GATATCGAAG CAGTACCCGG TGGTGGATCA CGCCTACGAT 
GCGATCGTCG TGGGAGCAGG AGGAGCCGGC TTGCGGGCGG CTTTTGGCCT GGTGGCTGAG 
GGATTTCGCA CGGCGGTGAT CACCAAGCTT TTCCCCACCC GCTCGCACAC GATCGCCGCC 
CAGGGCGGTA TCAATGCCGC TCTCGGCAAC ATGGAGGAGG ACGACTGGAA GTGGCACATG 
TACGACACGG TCAAGGGCTC CGATTGGCTG GGCGACCAGG ATGCCATCCA CTACATGACC 
CGCGAGGCGC CCAAGGCTGT CATTGAGCTG GAGAACTACG GCATGCCCTT CTCGCGTACC 
CAGGACGGTA AGATCTACCA GCGCGCCTTC GGTGGACAGA GTCTGAAGTT CGGCAAGGGC 
GGACAGGCCC ATCGCTGCTG TGCTGTGGCT GATCGTACTG GTCACTCGCT GCTGCACACG 
CTATACGGTC AATCGCTGAG CTACGACTGC AACTACTTTG TGGAGTACTT TGCCCTGGAT 
CTGATCTTCG AGGACGGCGA GTGCCGTGGT GTGCTGGCCC TGAACCTGGA GGATGGCACA 
CTGCACCGAT TCCGCGCTAA GAACACGGTC ATTGCCACCG GTGGATATGG ACGAGCATTC 
TTCTCCTGCA CCTCGGCGCA CACATGTACC GGTGACGGTA CTGCTATGGT TGCACGCCAG 
GGACTGCCCT CTCAGGATCT GGAGTTCGTG CAGTTCCATC CTACTGGCAT CTACGGCGCC 
GGATGTCTCA TCACCGAGGG CTGCCGCGGT GAGGGTGGTT ACCTGATCAA CGGTAATGGT 
GAGCGCTTCA TGGAGCGCTA TGCTCCTGTG GCCAAGGATC TGGCCTCTCG CGACGTCGTC 
TCGCGGTCGA TGACCATCGA GATCATGGAG GGTCGTGGCG CTGGACCCGA GAAGGATCAC 
GTGTACCTGC AACTGCACCA CTTGCCGCCC AAGCAGCTCG CGGAGCGTCT GCCTGGCATC 
TCCGAGACCG CCATGATCTT CGCCGGTGTT GATGTGACCC GTGAGCCCAT CCCCGTGTTG 
CCCACCGTGC ATTACAACAT GGGCGGTGTG CCGACTAACT ATCGCGGCCA GGTAATCACC 
ATTGACAAGG ATGGCAAGGA TGTGATTGTG CCGGGACTGT ATGCCGCTGG TGAGGCTGCT 
TCCAGCTCGG TGCATGGTGC CAACCGTCTG GGTGCCAACT CTCTGCTGGA TCTGGTGGTC 
TTCGGACGTG CATGCGCCAA GACCATCGCC GAGCTGAATA AGCCTGGTGC ACCGGCTCCC 
ACCCTCAAGG AAAACGCTGG CGAGGCCTCT GTTGCTAACC TCGATAAGCT GCGCCATGCC 
AACGGCCAGA TCACCACCGC CGATCTGCGT CTGAAGATGC AGAAGACCAT GCAGCATCAT 
GCTGCTGTGT TCCGCGATGG CCCCATCCTG CAGGACGGTG TGAACAAGAT GAAGGAGATC 
TACAAGCAGT TCAAGGACAT CAAGGTTGTC GATAGGTCGC TTATCTGGAA CTCCGATCTG 
GTGGAGACGC TGGAGCTGCA GAATCTGTTG GCCAATGCCC AGATGACTAT TGTGAGCGCT 
GAGGCGCGCA AGGAATCGCG AGGTGCCCAC GCCCGCGAGG ACTTCAAGGT TCGCGAGGAT 
GAATACGATT TCAGCAAGCC CCTGGATGGT CAGCAGAAGA AGCCCATGGA TCAGCACTGG 
CGCAAGCACA CGCTCTCGTG GGTGTGCAAT GACAACGGAG ACATTACGCT GGACTACAGA 
AACGTGATCG ATACCACGCT GGACAACGAA GTCTCTACTG TTCCACCAGC GATTCGCTCC 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SdhA cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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