Khc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Khc
Entrez Gene ID	36810
Full Name	Kinesin heavy chain
Synonyms	CG7765,DK,DKH,DmK,DmKHC,Dmel\CG7765,Dmkin,KHC,KIF 5A,KIF5,KIF5B,KIN,Kin,Kin-1,Kinesin-1,khc,kin,kinesin,kinesin-1,l(2)W12,l(2)k13219,l(2)k13314,l(2R)W12,pgs
General protein information	Preferred Names Kinesin heavy chain Names CG7765 gene product from transcript CG7765-RA CG7765-PA Khc-PA Kin-1 heavy chain Kinesin14 conventional kinesin heavy chain of kinesin kinesin kinesin 1 kinesin I kinesin alpha subunit kinesin alpha-chain kinesin heavy chain kinesin heavy chain I kinesin-1 kinesin-1 heavy chain partagas
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53A3-53A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057242.5	NP_476590.1	kinesin heavy chain

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIF5B	NP_004512.1
Bos taurus (cattle)	KIF5B	NP_001179294.1
Neurospora crassa	NCU09730	XP_964432.2
Danio rerio (zebrafish)	kif5ba	XP_005171465.1
Caenorhabditis elegans (roundworm)	unc-116	NP_498842.1
Canis lupus familiaris (dog)	KIF5B	XP_003638926.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000561	XP_310522.2
Macaca mulatta (Rhesus monkey)	KIF5B	XP_002805653.1
Drosophila melanogaster (fruit fly)	Khc	NP_476590.1
Magnaporthe oryzae (rice blast fungus)	MGG_01757	XP_003714755.1
Pan troglodytes (chimpanzee)	KIF5B	XP_507730.2
Mus musculus (house mouse)	Kif5b	NP_032474.2
Rattus norvegicus (Norway rat)	Kif5b	NP_476550.1
Gallus gallus (chicken)	KIF5B	XP_004939228.1
Danio rerio (zebrafish)	kif5bb	XP_002664065.1
Xenopus tropicalis (tropical clawed frog)	kif5b	NP_001122126.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Khc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Khc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10199	NM_057242.5 Latest version!	Drosophila melanogaster kinesin heavy chain (Khc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Khc ( NM_057242.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476590.1 Drosophila melanogaster kinesin heavy chain (Khc), mRNA.

Price & Availability↑

CloneID	OFa10199
Clone ID Related Accession (Same CDS sequence)	NM_057242.5
Accession Version	NM_057242.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin heavy chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057242.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCGG AACGAGAGAT TCCCGCCGAG GACAGCATCA AAGTGGTCTG CCGATTCCGA 
CCGCTGAACG ACAGCGAAGA GAAGGCCGGC TCCAAGTTCG TGGTCAAGTT CCCCAACAAT 
GTGGAGGAGA ACTGCATATC CATAGCGGGC AAGGTGTATT TGTTCGACAA GGTCTTCAAA 
CCGAATGCAT CCCAGGAGAA GGTCTACAAT GAGGCGGCCA AGTCCATTGT TACGGATGTC 
CTGGCCGGGT ACAATGGAAC GATATTCGCA TATGGTCAGA CGTCCTCCGG AAAAACGCAT 
ACGATGGAGG GCGTGATCGG GGACTCCGTA AAACAGGGTA TCATACCACG TATCGTCAAC 
GACATTTTCA ATCACATCTA CGCGATGGAG GTGAACCTGG AGTTTCACAT CAAGGTCTCC 
TACTACGAGA TCTACATGGA CAAGATTCGA GATCTGTTGG ACGTCTCCAA GGTGAACTTA 
AGCGTGCACG AGGACAAGAA CCGGGTGCCG TACGTCAAGG GCGCTACGGA ACGGTTCGTC 
TCGTCGCCGG AGGATGTTTT CGAGGTGATC GAGGAGGGCA AATCCAATCG TCACATCGCT 
GTGACAAACA TGAACGAGCA TTCTTCGCGA TCCCACTCAG TATTCCTTAT CAATGTGAAG 
CAGGAGAACC TGGAGAACCA GAAGAAACTA TCCGGCAAAC TCTACCTGGT GGATTTGGCC 
GGTTCCGAGA AGGTTTCCAA GACTGGAGCG GAGGGAACCG TTCTTGATGA AGCCAAGAAC 
ATCAACAAGT CGCTGTCGGC CTTGGGCAAC GTAATTTCAG CCCTGGCGGA CGGAAACAAA 
ACGCACATCC CCTACCGTGA TTCCAAGCTC ACGCGCATCC TGCAGGAGTC GCTGGGAGGC 
AATGCACGCA CAACCATCGT CATCTGCTGC TCTCCAGCCA GTTTCAACGA ATCTGAAACG 
AAGTCAACGC TGGACTTCGG TCGTAGAGCC AAGACAGTGA AGAACGTGGT CTGCGTTAAC 
GAGGAGCTTA CTGCCGAGGA ATGGAAGCGA CGCTATGAAA AGGAGAAGGA AAAGAACGCC 
CGACTAAAGG GTAAGGTGGA GAAGCTGGAG ATCGAGCTTG CGCGCTGGAG AGCGGGTGAA 
ACTGTTAAGG CGGAGGAGCA AATCAACATG GAGGATCTCA TGGAGGCAAG CACGCCCAAC 
CTGGAAGTGG AGGCAGCACA GACGGCGGCG GCCGAGGCCG CTTTGGCCGC CCAGCGAACG 
GCTCTCGCCA ATATGTCCGC ATCGGTTGCC GTGAACGAGC AGGCCAGGCT GGCTACAGAG 
TGCGAGCGTC TCTACCAGCA GCTGGACGAC AAGGATGAGG AGATCAATCA GCAGAGCCAG 
TACGCCGAGC AGCTCAAGGA GCAGGTGATG GAGCAGGAGG AACTCATCGC TAACGCTCGG 
CGTGAGTATG AGACTTTGCA GTCGGAGATG GCGCGAATCC AACAGGAGAA CGAGTCCGCC 
AAGGAAGAGG TTAAGGAGGT GCTCCAAGCT CTCGAAGAGC TGGCTGTAAA CTACGACCAG 
AAATCCCAGG AGATCGATAA CAAGAACAAG GATATCGATG CCCTCAACGA GGAGCTGCAG 
CAGAAGCAGT CTGTGTTCAA CGCCGCCTCC ACAGAGCTAC AGCAGCTCAA GGACATGTCC 
TCACACCAGA AGAAGCGCAT CACGGAAATG CTAACCAACC TACTGCGCGA CCTTGGCGAA 
GTGGGCCAGG CCATTGCCCC CGGCGAGTCC AGCATTGACC TCAAGATGAG TGCTCTGGCT 
GGCACGGATG CCAGCAAGGT GGAGGAAGAT TTCACCATGG CGCGTTTGTT TATCAGCAAG 
ATGAAGACGG AGGCCAAGAA CATTGCCCAG CGATGCTCCA ACATGGAAAC ACAGCAGGCT 
GACTCCAACA AGAAGATCTC CGAATATGAG AAAGATCTGG GCGAGTACCG GCTACTCATT 
TCGCAGCACG AGGCACGCAT GAAGTCGCTG CAGGAGTCGA TGCGGGAGGC AGAGAACAAG 
AAGCGCACGC TCGAGGAACA AATCGATTCG CTGCGCGAGG AATGCGCCAA GCTCAAGGCC 
GCCGAGCACG TTTCCGCCGT TAACGCCGAG GAGAAACAGC GGGCTGAGGA GCTGCGCTCC 
ATGTTCGATT CTCAGATGGA CGAGCTACGC GAAGCCCACA CCCGGCAGGT GTCCGAGCTC 
CGGGATGAGA TTGCCGCCAA GCAGCACGAA ATGGACGAGA TGAAGGATGT CCATCAAAAG 
CTGCTCTTGG CGCACCAACA GATGACGGCC GACTACGAGA AGGTGCGCCA GGAGGATGCC 
GAGAAGTCCA GCGAGCTTCA GAACATCATC CTCACCAACG AGCGTCGGGA GCAAGCGCGC 
AAAGACCTCA AGGGCCTGGA GGACACGGTG GCCAAGGAGT TGCAGACGCT ACACAACCTG 
CGAAAACTTT TCGTTCAGGA TCTACAGCAA CGAATCCGAA AGAATGTCGT AAACGAGGAG 
AGCGAGGAGG ACGGTGGATC ACTCGCGCAG AAACAGAAGA TTTCCTTCTT GGAGAACAAC 
CTCGACCAGC TGACCAAGGT GCACAAGCAA TTGGTGCGGG ACAACGCCGA TCTGCGGTGC 
GAGCTGCCCA AGCTGGAGAA GCGTCTACGC TGTACCATGG AGCGGGTGAA AGCTCTGGAG 
ACAGCGCTCA AGGAGGCGAA GGAGGGCGCA ATGCGGGATC GCAAGCGCTA CCAATACGAG 
GTGGACCGCA TCAAGGAGGC GGTGCGACAG AAGCATCTGG GCAGACGTGG CCCACAGGCA 
CAGATCGCCA AGCCGATCCG GTCCGGCCAG GGTGCAATCG CCATTCGTGG TGGTGGCGCC 
GTTGGAGGAC CATCCCCGCT GGCCCAGGTT AATCCTGTCA ACTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476590.1
CDS	487..3414
Translation	MSAEREIPAEDSIKVVCRFRPLNDSEEKAGSKFVVKFPNNVEENCISIAGKVYLFDKVFKPNASQEKVYNEAAKSIVTDVLAGYNGTIFAYGQTSSGKTHTMEGVIGDSVKQGIIPRIVNDIFNHIYAMEVNLEFHIKVSYYEIYMDKIRDLLDVSKVNLSVHEDKNRVPYVKGATERFVSSPEDVFEVIEEGKSNRHIAVTNMNEHSSRSHSVFLINVKQENLENQKKLSGKLYLVDLAGSEKVSKTGAEGTVLDEAKNINKSLSALGNVISALADGNKTHIPYRDSKLTRILQESLGGNARTTIVICCSPASFNESETKSTLDFGRRAKTVKNVVCVNEELTAEEWKRRYEKEKEKNARLKGKVEKLEIELARWRAGETVKAEEQINMEDLMEASTPNLEVEAAQTAAAEAALAAQRTALANMSASVAVNEQARLATECERLYQQLDDKDEEINQQSQYAEQLKEQVMEQEELIANARREYETLQSEMARIQQENESAKEEVKEVLQALEELAVNYDQKSQEIDNKNKDIDALNEELQQKQSVFNAASTELQQLKDMSSHQKKRITEMLTNLLRDLGEVGQAIAPGESSIDLKMSALAGTDASKVEEDFTMARLFISKMKTEAKNIAQRCSNMETQQADSNKKISEYEKDLGEYRLLISQHEARMKSLQESMREAENKKRTLEEQIDSLREECAKLKAAEHVSAVNAEEKQRAEELRSMFDSQMDELREAHTRQVSELRDEIAAKQHEMDEMKDVHQKLLLAHQQMTADYEKVRQEDAEKSSELQNIILTNERREQARKDLKGLEDTVAKELQTLHNLRKLFVQDLQQRIRKNVVNEESEEDGGSLAQKQKISFLENNLDQLTKVHKQLVRDNADLRCELPKLEKRLRCTMERVKALETALKEAKEGAMRDRKRYQYEVDRIKEAVRQKHLGRRGPQAQIAKPIRSGQGAIAIRGGGAVGGPSPLAQVNPVNS

Target ORF information:

RefSeq Version	NM_057242.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin heavy chain (Khc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057242.5

ORF Insert Sequence:

ATGTCCGCGG AACGAGAGAT TCCCGCCGAG GACAGCATCA AAGTGGTCTG CCGATTCCGA 
CCGCTGAACG ACAGCGAAGA GAAGGCCGGC TCCAAGTTCG TGGTCAAGTT CCCCAACAAT 
GTGGAGGAGA ACTGCATATC CATAGCGGGC AAGGTGTATT TGTTCGACAA GGTCTTCAAA 
CCGAATGCAT CCCAGGAGAA GGTCTACAAT GAGGCGGCCA AGTCCATTGT TACGGATGTC 
CTGGCCGGGT ACAATGGAAC GATATTCGCA TATGGTCAGA CGTCCTCCGG AAAAACGCAT 
ACGATGGAGG GCGTGATCGG GGACTCCGTA AAACAGGGTA TCATACCACG TATCGTCAAC 
GACATTTTCA ATCACATCTA CGCGATGGAG GTGAACCTGG AGTTTCACAT CAAGGTCTCC 
TACTACGAGA TCTACATGGA CAAGATTCGA GATCTGTTGG ACGTCTCCAA GGTGAACTTA 
AGCGTGCACG AGGACAAGAA CCGGGTGCCG TACGTCAAGG GCGCTACGGA ACGGTTCGTC 
TCGTCGCCGG AGGATGTTTT CGAGGTGATC GAGGAGGGCA AATCCAATCG TCACATCGCT 
GTGACAAACA TGAACGAGCA TTCTTCGCGA TCCCACTCAG TATTCCTTAT CAATGTGAAG 
CAGGAGAACC TGGAGAACCA GAAGAAACTA TCCGGCAAAC TCTACCTGGT GGATTTGGCC 
GGTTCCGAGA AGGTTTCCAA GACTGGAGCG GAGGGAACCG TTCTTGATGA AGCCAAGAAC 
ATCAACAAGT CGCTGTCGGC CTTGGGCAAC GTAATTTCAG CCCTGGCGGA CGGAAACAAA 
ACGCACATCC CCTACCGTGA TTCCAAGCTC ACGCGCATCC TGCAGGAGTC GCTGGGAGGC 
AATGCACGCA CAACCATCGT CATCTGCTGC TCTCCAGCCA GTTTCAACGA ATCTGAAACG 
AAGTCAACGC TGGACTTCGG TCGTAGAGCC AAGACAGTGA AGAACGTGGT CTGCGTTAAC 
GAGGAGCTTA CTGCCGAGGA ATGGAAGCGA CGCTATGAAA AGGAGAAGGA AAAGAACGCC 
CGACTAAAGG GTAAGGTGGA GAAGCTGGAG ATCGAGCTTG CGCGCTGGAG AGCGGGTGAA 
ACTGTTAAGG CGGAGGAGCA AATCAACATG GAGGATCTCA TGGAGGCAAG CACGCCCAAC 
CTGGAAGTGG AGGCAGCACA GACGGCGGCG GCCGAGGCCG CTTTGGCCGC CCAGCGAACG 
GCTCTCGCCA ATATGTCCGC ATCGGTTGCC GTGAACGAGC AGGCCAGGCT GGCTACAGAG 
TGCGAGCGTC TCTACCAGCA GCTGGACGAC AAGGATGAGG AGATCAATCA GCAGAGCCAG 
TACGCCGAGC AGCTCAAGGA GCAGGTGATG GAGCAGGAGG AACTCATCGC TAACGCTCGG 
CGTGAGTATG AGACTTTGCA GTCGGAGATG GCGCGAATCC AACAGGAGAA CGAGTCCGCC 
AAGGAAGAGG TTAAGGAGGT GCTCCAAGCT CTCGAAGAGC TGGCTGTAAA CTACGACCAG 
AAATCCCAGG AGATCGATAA CAAGAACAAG GATATCGATG CCCTCAACGA GGAGCTGCAG 
CAGAAGCAGT CTGTGTTCAA CGCCGCCTCC ACAGAGCTAC AGCAGCTCAA GGACATGTCC 
TCACACCAGA AGAAGCGCAT CACGGAAATG CTAACCAACC TACTGCGCGA CCTTGGCGAA 
GTGGGCCAGG CCATTGCCCC CGGCGAGTCC AGCATTGACC TCAAGATGAG TGCTCTGGCT 
GGCACGGATG CCAGCAAGGT GGAGGAAGAT TTCACCATGG CGCGTTTGTT TATCAGCAAG 
ATGAAGACGG AGGCCAAGAA CATTGCCCAG CGATGCTCCA ACATGGAAAC ACAGCAGGCT 
GACTCCAACA AGAAGATCTC CGAATATGAG AAAGATCTGG GCGAGTACCG GCTACTCATT 
TCGCAGCACG AGGCACGCAT GAAGTCGCTG CAGGAGTCGA TGCGGGAGGC AGAGAACAAG 
AAGCGCACGC TCGAGGAACA AATCGATTCG CTGCGCGAGG AATGCGCCAA GCTCAAGGCC 
GCCGAGCACG TTTCCGCCGT TAACGCCGAG GAGAAACAGC GGGCTGAGGA GCTGCGCTCC 
ATGTTCGATT CTCAGATGGA CGAGCTACGC GAAGCCCACA CCCGGCAGGT GTCCGAGCTC 
CGGGATGAGA TTGCCGCCAA GCAGCACGAA ATGGACGAGA TGAAGGATGT CCATCAAAAG 
CTGCTCTTGG CGCACCAACA GATGACGGCC GACTACGAGA AGGTGCGCCA GGAGGATGCC 
GAGAAGTCCA GCGAGCTTCA GAACATCATC CTCACCAACG AGCGTCGGGA GCAAGCGCGC 
AAAGACCTCA AGGGCCTGGA GGACACGGTG GCCAAGGAGT TGCAGACGCT ACACAACCTG 
CGAAAACTTT TCGTTCAGGA TCTACAGCAA CGAATCCGAA AGAATGTCGT AAACGAGGAG 
AGCGAGGAGG ACGGTGGATC ACTCGCGCAG AAACAGAAGA TTTCCTTCTT GGAGAACAAC 
CTCGACCAGC TGACCAAGGT GCACAAGCAA TTGGTGCGGG ACAACGCCGA TCTGCGGTGC 
GAGCTGCCCA AGCTGGAGAA GCGTCTACGC TGTACCATGG AGCGGGTGAA AGCTCTGGAG 
ACAGCGCTCA AGGAGGCGAA GGAGGGCGCA ATGCGGGATC GCAAGCGCTA CCAATACGAG 
GTGGACCGCA TCAAGGAGGC GGTGCGACAG AAGCATCTGG GCAGACGTGG CCCACAGGCA 
CAGATCGCCA AGCCGATCCG GTCCGGCCAG GGTGCAATCG CCATTCGTGG TGGTGGCGCC 
GTTGGAGGAC CATCCCCGCT GGCCCAGGTT AATCCTGTCA ACTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Khc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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