Etf-QO cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Etf-QO
Entrez Gene ID	36031
Full Name	Electron transfer flavoprotein-ubiquinone oxidoreductase
Synonyms	CG 12140,CG12140,Dmel\CG12140,ETF:QO,ETFD,ETFDH
General protein information	Preferred Names Electron transfer flavoprotein-ubiquinone oxidoreductase Names CG12140 gene product from transcript CG12140-RA CG12140-PA CG12140-PB Etf-QO-PA Etf-QO-PB electron transfer flavoprotein-ubiquinone oxidoreductase electron transfer flavoprotein:ubiquinone oxidoreductase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 46C4-46C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259302.1	NP_001246231.1	electron transfer flavoprotein-ubiquinone oxidoreductase, isoform B
NM_136692.1	NP_610536.1	electron transfer flavoprotein-ubiquinone oxidoreductase, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	etfdh	NP_001004598.1
Caenorhabditis elegans (roundworm)	let-721	NP_498415.2
Saccharomyces cerevisiae (baker's yeast)	CIR2	NP_015001.1
Macaca mulatta (Rhesus monkey)	ETFDH	XP_001097120.1
Mus musculus (house mouse)	Etfdh	NP_080070.2
Magnaporthe oryzae (rice blast fungus)	MGG_08880	XP_003713904.1
Gallus gallus (chicken)	ETFDH	NP_001026705.1
Arabidopsis thaliana (thale cress)	ETFQO	NP_181868.1
Xenopus tropicalis (tropical clawed frog)	etfdh	NP_001005631.1
Eremothecium gossypii	AGOS_ADR387W	NP_984483.2
Schizosaccharomyces pombe (fission yeast)	SPAC20G8.04c	NP_593321.1
Kluyveromyces lactis	KLLA0C12991g	XP_452779.1
Rattus norvegicus (Norway rat)	Etfdh	NP_942037.2
Drosophila melanogaster (fruit fly)	Etf-QO	NP_610536.1
Neurospora crassa	NCU04768	XP_960217.1
Oryza sativa (rice)	Os10g0516300	NP_001065062.1
Homo sapiens (human)	ETFDH	NP_004444.2
Canis lupus familiaris (dog)	ETFDH	XP_853781.2
Bos taurus (cattle)	ETFDH	NP_001070598.1
Pan troglodytes (chimpanzee)	ETFDH	XP_517508.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005894	XP_315923.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Etf-QO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Etf-QO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09419	NM_136692.1 Latest version!	Drosophila melanogaster electron transfer flavoprotein-ubiquinone oxidoreductase, transcript variant A (Etf-QO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa09419	NM_001259302.1 Latest version!	Drosophila melanogaster electron transfer flavoprotein-ubiquinone oxidoreductase, transcript variant B (Etf-QO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Etf-QO ( NM_001259302.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246231.1 Drosophila melanogaster electron transfer flavoprotein-ubiquinone oxidoreductase, transcript variant B (Etf-QO), mRNA.

Price & Availability↑

CloneID	OFa09419
Clone ID Related Accession (Same CDS sequence)	NM_001259302.1 , NM_136692.1
Accession Version	NM_001259302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCAC TCATGAAAAT GCACAGAGTT CGGAGACTCT TCAGCCCCGC TCTGGCTCGC 
AGCATCTCCG ATGTGGCCAA ATATCCCAGG ATAACCACCC ACTATACGCT GAATCCCCGC 
GAGAAGGATG AGCGCTGGAC GGAAGTGGAT ATGGAGCGTT GTGTTGAGGA GGTGGACCTG 
GTCATCGTCG GTGGAGGACC TGCTGGAATG TCGGCGGCTA TTAGGGCGAA GCAACTGGCG 
GCGGAAAAGG ATCAGGAAAT CCGCGTCTGC GTAGTGGAAA AGGCCGCCGA AGTTGGCGGA 
CATATTCTCT CCGGCGCCGT CATAGATCCA ATCTCGCTGA ACGAGCTTAT CCCGGACTGG 
CAAGAGCAGG GAGCTCCTCT AAACACACCC GTGACCAAGG ATACTTTCTC CTTCCTCACC 
GGATCCGGCC GCATCTCCAT TCCGGTCTTT AAGGGCTGGC CCATGGACAA CCATGGCAAC 
TACGTAGTTC GCTTGGGCCA CTTGGTCAAG TGGCTTGGAG ATCAGGCGGA GGCTCTGGGC 
GTGGAGATCT ATCCCGGCTG TGCTGCCTCC GAGGTGCTCT TCCACGAGGA TGGCAGTGTC 
AAGGGCATTG CCACCAACGA TGTGGGCATT GCCAAGAGCG GAGCTCCCAA GGACACCTTT 
GCACGTGGCA TGGAGTTGCA CGCCAAGACT ACCATCTTTG CCGAAGGCTG CCGGGGTCAC 
CTGACCAAGC AGATTATGCA GAAGTTCGGA CTTAACGAGG GCAGCGAACC ACAGGCCTAT 
GGAATCGGTC TGAAAGAGGT CTGGGAGATT GCCCCCGAGA AGCATCAACC TGGTTTGGTG 
GAGCATACCA TTGGCTGGCC TTTGGACCGC TTCACATATG GCGGCTCCTT CTTGTACCAT 
TTGAACGAGC CCACACCCAC CATTGCAGTG GGCTTTGTGG TGGGCTTGAA CTACAAGAAC 
CCCTGGCTCA GTCCCTTCCA GGAGTTCCAG CGTTTCAAGA CGCATCCCAA GGTGCGAGAC 
GTCTTCGAGG GCGCCACTCG AATTGCCTAT GGAGCACGAG CAATCAATGA GGGTGGCTTC 
CAAAGTCTGC CGCAGAAGCT GTCCTTTCCG GGAGGTTGCC TTGTGGGCTG CAGTGCTGGC 
TTTTTGAATG TCCCTCGCAT TAAGGGCTCC CATTACGCGA TGAAGAGCGG CATGCTGGCG 
GCGGAGAGTG CCTTTGAAGC CATCAATGCG GATGCACAAT CGACGGCTGG TGTGGAACCC 
ACAGGCTATG CGGAGAAAAT CAAGGACTCC TTTGTATGGA AGGATCTGTA CAGTGTGCGA 
AATGTGCACC CTTCTTTCCA CAATCCATTG GGACTGTACG GTGGTCTTGT GCTCAGTGGC 
TTCTCCATTT TCATGGGCGG TCGGGAACCA TGGACCCTAA AACACGGTCC CCAGGATCAC 
GAGTCCTTGA AGCCAGCTAG CTCCTGCCCG CCCATTGTTT ACCCCAAGCC CGATGGCAAG 
ATCTCCTTCG ATCTGCTGTC TTCGGTAGCA CTGACGGGCA CAAACCACGA GGGCGACCAG 
CCCGCCCATC TGACCCTCAA GGACGACCGC ATCCCCGTGG ACCATAATTT GGCGCTGTAC 
GAGGGACCCG AGCAGAGATT CTGCCCCGCT GGCGTTTACG AGTACGTGCC CAACGAGGAG 
GGCGGCAACA TGAAGCTGCA AATTAACGCC CAGAACTGCA TCCACTGCAA GACCTGTGAT 
ATCAAGGACC CCAAGCAGAA CATCAACTGG GTGGTGCCCG AGGGAGGTGG CGGTCCCGCC 
TACAACGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246231.1
CDS	306..2120
Translation	MSALMKMHRVRRLFSPALARSISDVAKYPRITTHYTLNPREKDERWTEVDMERCVEEVDLVIVGGGPAGMSAAIRAKQLAAEKDQEIRVCVVEKAAEVGGHILSGAVIDPISLNELIPDWQEQGAPLNTPVTKDTFSFLTGSGRISIPVFKGWPMDNHGNYVVRLGHLVKWLGDQAEALGVEIYPGCAASEVLFHEDGSVKGIATNDVGIAKSGAPKDTFARGMELHAKTTIFAEGCRGHLTKQIMQKFGLNEGSEPQAYGIGLKEVWEIAPEKHQPGLVEHTIGWPLDRFTYGGSFLYHLNEPTPTIAVGFVVGLNYKNPWLSPFQEFQRFKTHPKVRDVFEGATRIAYGARAINEGGFQSLPQKLSFPGGCLVGCSAGFLNVPRIKGSHYAMKSGMLAAESAFEAINADAQSTAGVEPTGYAEKIKDSFVWKDLYSVRNVHPSFHNPLGLYGGLVLSGFSIFMGGREPWTLKHGPQDHESLKPASSCPPIVYPKPDGKISFDLLSSVALTGTNHEGDQPAHLTLKDDRIPVDHNLALYEGPEQRFCPAGVYEYVPNEEGGNMKLQINAQNCIHCKTCDIKDPKQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	NM_001259302.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster electron transfer flavoprotein-ubiquinone oxidoreductase, transcript variant B (Etf-QO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259302.1

ORF Insert Sequence:

ATGTCGGCAC TCATGAAAAT GCACAGAGTT CGGAGACTCT TCAGCCCCGC TCTGGCTCGC 
AGCATCTCCG ATGTGGCCAA ATATCCCAGG ATAACCACCC ACTATACGCT GAATCCCCGC 
GAGAAGGATG AGCGCTGGAC GGAAGTGGAT ATGGAGCGTT GTGTTGAGGA GGTGGACCTG 
GTCATCGTCG GTGGAGGACC TGCTGGAATG TCGGCGGCTA TTAGGGCGAA GCAACTGGCG 
GCGGAAAAGG ATCAGGAAAT CCGCGTCTGC GTAGTGGAAA AGGCCGCCGA AGTTGGCGGA 
CATATTCTCT CCGGCGCCGT CATAGATCCA ATCTCGCTGA ACGAGCTTAT CCCGGACTGG 
CAAGAGCAGG GAGCTCCTCT AAACACACCC GTGACCAAGG ATACTTTCTC CTTCCTCACC 
GGATCCGGCC GCATCTCCAT TCCGGTCTTT AAGGGCTGGC CCATGGACAA CCATGGCAAC 
TACGTAGTTC GCTTGGGCCA CTTGGTCAAG TGGCTTGGAG ATCAGGCGGA GGCTCTGGGC 
GTGGAGATCT ATCCCGGCTG TGCTGCCTCC GAGGTGCTCT TCCACGAGGA TGGCAGTGTC 
AAGGGCATTG CCACCAACGA TGTGGGCATT GCCAAGAGCG GAGCTCCCAA GGACACCTTT 
GCACGTGGCA TGGAGTTGCA CGCCAAGACT ACCATCTTTG CCGAAGGCTG CCGGGGTCAC 
CTGACCAAGC AGATTATGCA GAAGTTCGGA CTTAACGAGG GCAGCGAACC ACAGGCCTAT 
GGAATCGGTC TGAAAGAGGT CTGGGAGATT GCCCCCGAGA AGCATCAACC TGGTTTGGTG 
GAGCATACCA TTGGCTGGCC TTTGGACCGC TTCACATATG GCGGCTCCTT CTTGTACCAT 
TTGAACGAGC CCACACCCAC CATTGCAGTG GGCTTTGTGG TGGGCTTGAA CTACAAGAAC 
CCCTGGCTCA GTCCCTTCCA GGAGTTCCAG CGTTTCAAGA CGCATCCCAA GGTGCGAGAC 
GTCTTCGAGG GCGCCACTCG AATTGCCTAT GGAGCACGAG CAATCAATGA GGGTGGCTTC 
CAAAGTCTGC CGCAGAAGCT GTCCTTTCCG GGAGGTTGCC TTGTGGGCTG CAGTGCTGGC 
TTTTTGAATG TCCCTCGCAT TAAGGGCTCC CATTACGCGA TGAAGAGCGG CATGCTGGCG 
GCGGAGAGTG CCTTTGAAGC CATCAATGCG GATGCACAAT CGACGGCTGG TGTGGAACCC 
ACAGGCTATG CGGAGAAAAT CAAGGACTCC TTTGTATGGA AGGATCTGTA CAGTGTGCGA 
AATGTGCACC CTTCTTTCCA CAATCCATTG GGACTGTACG GTGGTCTTGT GCTCAGTGGC 
TTCTCCATTT TCATGGGCGG TCGGGAACCA TGGACCCTAA AACACGGTCC CCAGGATCAC 
GAGTCCTTGA AGCCAGCTAG CTCCTGCCCG CCCATTGTTT ACCCCAAGCC CGATGGCAAG 
ATCTCCTTCG ATCTGCTGTC TTCGGTAGCA CTGACGGGCA CAAACCACGA GGGCGACCAG 
CCCGCCCATC TGACCCTCAA GGACGACCGC ATCCCCGTGG ACCATAATTT GGCGCTGTAC 
GAGGGACCCG AGCAGAGATT CTGCCCCGCT GGCGTTTACG AGTACGTGCC CAACGAGGAG 
GGCGGCAACA TGAAGCTGCA AATTAACGCC CAGAACTGCA TCCACTGCAA GACCTGTGAT 
ATCAAGGACC CCAAGCAGAA CATCAACTGG GTGGTGCCCG AGGGAGGTGG CGGTCCCGCC 
TACAACGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Etf-QO ( NM_136692.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610536.1 Drosophila melanogaster electron transfer flavoprotein-ubiquinone oxidoreductase, transcript variant A (Etf-QO), mRNA.

Price & Availability↑

CloneID	OFa09419
Clone ID Related Accession (Same CDS sequence)	NM_001259302.1 , NM_136692.1
Accession Version	NM_136692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCAC TCATGAAAAT GCACAGAGTT CGGAGACTCT TCAGCCCCGC TCTGGCTCGC 
AGCATCTCCG ATGTGGCCAA ATATCCCAGG ATAACCACCC ACTATACGCT GAATCCCCGC 
GAGAAGGATG AGCGCTGGAC GGAAGTGGAT ATGGAGCGTT GTGTTGAGGA GGTGGACCTG 
GTCATCGTCG GTGGAGGACC TGCTGGAATG TCGGCGGCTA TTAGGGCGAA GCAACTGGCG 
GCGGAAAAGG ATCAGGAAAT CCGCGTCTGC GTAGTGGAAA AGGCCGCCGA AGTTGGCGGA 
CATATTCTCT CCGGCGCCGT CATAGATCCA ATCTCGCTGA ACGAGCTTAT CCCGGACTGG 
CAAGAGCAGG GAGCTCCTCT AAACACACCC GTGACCAAGG ATACTTTCTC CTTCCTCACC 
GGATCCGGCC GCATCTCCAT TCCGGTCTTT AAGGGCTGGC CCATGGACAA CCATGGCAAC 
TACGTAGTTC GCTTGGGCCA CTTGGTCAAG TGGCTTGGAG ATCAGGCGGA GGCTCTGGGC 
GTGGAGATCT ATCCCGGCTG TGCTGCCTCC GAGGTGCTCT TCCACGAGGA TGGCAGTGTC 
AAGGGCATTG CCACCAACGA TGTGGGCATT GCCAAGAGCG GAGCTCCCAA GGACACCTTT 
GCACGTGGCA TGGAGTTGCA CGCCAAGACT ACCATCTTTG CCGAAGGCTG CCGGGGTCAC 
CTGACCAAGC AGATTATGCA GAAGTTCGGA CTTAACGAGG GCAGCGAACC ACAGGCCTAT 
GGAATCGGTC TGAAAGAGGT CTGGGAGATT GCCCCCGAGA AGCATCAACC TGGTTTGGTG 
GAGCATACCA TTGGCTGGCC TTTGGACCGC TTCACATATG GCGGCTCCTT CTTGTACCAT 
TTGAACGAGC CCACACCCAC CATTGCAGTG GGCTTTGTGG TGGGCTTGAA CTACAAGAAC 
CCCTGGCTCA GTCCCTTCCA GGAGTTCCAG CGTTTCAAGA CGCATCCCAA GGTGCGAGAC 
GTCTTCGAGG GCGCCACTCG AATTGCCTAT GGAGCACGAG CAATCAATGA GGGTGGCTTC 
CAAAGTCTGC CGCAGAAGCT GTCCTTTCCG GGAGGTTGCC TTGTGGGCTG CAGTGCTGGC 
TTTTTGAATG TCCCTCGCAT TAAGGGCTCC CATTACGCGA TGAAGAGCGG CATGCTGGCG 
GCGGAGAGTG CCTTTGAAGC CATCAATGCG GATGCACAAT CGACGGCTGG TGTGGAACCC 
ACAGGCTATG CGGAGAAAAT CAAGGACTCC TTTGTATGGA AGGATCTGTA CAGTGTGCGA 
AATGTGCACC CTTCTTTCCA CAATCCATTG GGACTGTACG GTGGTCTTGT GCTCAGTGGC 
TTCTCCATTT TCATGGGCGG TCGGGAACCA TGGACCCTAA AACACGGTCC CCAGGATCAC 
GAGTCCTTGA AGCCAGCTAG CTCCTGCCCG CCCATTGTTT ACCCCAAGCC CGATGGCAAG 
ATCTCCTTCG ATCTGCTGTC TTCGGTAGCA CTGACGGGCA CAAACCACGA GGGCGACCAG 
CCCGCCCATC TGACCCTCAA GGACGACCGC ATCCCCGTGG ACCATAATTT GGCGCTGTAC 
GAGGGACCCG AGCAGAGATT CTGCCCCGCT GGCGTTTACG AGTACGTGCC CAACGAGGAG 
GGCGGCAACA TGAAGCTGCA AATTAACGCC CAGAACTGCA TCCACTGCAA GACCTGTGAT 
ATCAAGGACC CCAAGCAGAA CATCAACTGG GTGGTGCCCG AGGGAGGTGG CGGTCCCGCC 
TACAACGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610536.1
CDS	363..2177
Translation	MSALMKMHRVRRLFSPALARSISDVAKYPRITTHYTLNPREKDERWTEVDMERCVEEVDLVIVGGGPAGMSAAIRAKQLAAEKDQEIRVCVVEKAAEVGGHILSGAVIDPISLNELIPDWQEQGAPLNTPVTKDTFSFLTGSGRISIPVFKGWPMDNHGNYVVRLGHLVKWLGDQAEALGVEIYPGCAASEVLFHEDGSVKGIATNDVGIAKSGAPKDTFARGMELHAKTTIFAEGCRGHLTKQIMQKFGLNEGSEPQAYGIGLKEVWEIAPEKHQPGLVEHTIGWPLDRFTYGGSFLYHLNEPTPTIAVGFVVGLNYKNPWLSPFQEFQRFKTHPKVRDVFEGATRIAYGARAINEGGFQSLPQKLSFPGGCLVGCSAGFLNVPRIKGSHYAMKSGMLAAESAFEAINADAQSTAGVEPTGYAEKIKDSFVWKDLYSVRNVHPSFHNPLGLYGGLVLSGFSIFMGGREPWTLKHGPQDHESLKPASSCPPIVYPKPDGKISFDLLSSVALTGTNHEGDQPAHLTLKDDRIPVDHNLALYEGPEQRFCPAGVYEYVPNEEGGNMKLQINAQNCIHCKTCDIKDPKQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	NM_136692.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster electron transfer flavoprotein-ubiquinone oxidoreductase, transcript variant A (Etf-QO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136692.1

ORF Insert Sequence:

ATGTCGGCAC TCATGAAAAT GCACAGAGTT CGGAGACTCT TCAGCCCCGC TCTGGCTCGC 
AGCATCTCCG ATGTGGCCAA ATATCCCAGG ATAACCACCC ACTATACGCT GAATCCCCGC 
GAGAAGGATG AGCGCTGGAC GGAAGTGGAT ATGGAGCGTT GTGTTGAGGA GGTGGACCTG 
GTCATCGTCG GTGGAGGACC TGCTGGAATG TCGGCGGCTA TTAGGGCGAA GCAACTGGCG 
GCGGAAAAGG ATCAGGAAAT CCGCGTCTGC GTAGTGGAAA AGGCCGCCGA AGTTGGCGGA 
CATATTCTCT CCGGCGCCGT CATAGATCCA ATCTCGCTGA ACGAGCTTAT CCCGGACTGG 
CAAGAGCAGG GAGCTCCTCT AAACACACCC GTGACCAAGG ATACTTTCTC CTTCCTCACC 
GGATCCGGCC GCATCTCCAT TCCGGTCTTT AAGGGCTGGC CCATGGACAA CCATGGCAAC 
TACGTAGTTC GCTTGGGCCA CTTGGTCAAG TGGCTTGGAG ATCAGGCGGA GGCTCTGGGC 
GTGGAGATCT ATCCCGGCTG TGCTGCCTCC GAGGTGCTCT TCCACGAGGA TGGCAGTGTC 
AAGGGCATTG CCACCAACGA TGTGGGCATT GCCAAGAGCG GAGCTCCCAA GGACACCTTT 
GCACGTGGCA TGGAGTTGCA CGCCAAGACT ACCATCTTTG CCGAAGGCTG CCGGGGTCAC 
CTGACCAAGC AGATTATGCA GAAGTTCGGA CTTAACGAGG GCAGCGAACC ACAGGCCTAT 
GGAATCGGTC TGAAAGAGGT CTGGGAGATT GCCCCCGAGA AGCATCAACC TGGTTTGGTG 
GAGCATACCA TTGGCTGGCC TTTGGACCGC TTCACATATG GCGGCTCCTT CTTGTACCAT 
TTGAACGAGC CCACACCCAC CATTGCAGTG GGCTTTGTGG TGGGCTTGAA CTACAAGAAC 
CCCTGGCTCA GTCCCTTCCA GGAGTTCCAG CGTTTCAAGA CGCATCCCAA GGTGCGAGAC 
GTCTTCGAGG GCGCCACTCG AATTGCCTAT GGAGCACGAG CAATCAATGA GGGTGGCTTC 
CAAAGTCTGC CGCAGAAGCT GTCCTTTCCG GGAGGTTGCC TTGTGGGCTG CAGTGCTGGC 
TTTTTGAATG TCCCTCGCAT TAAGGGCTCC CATTACGCGA TGAAGAGCGG CATGCTGGCG 
GCGGAGAGTG CCTTTGAAGC CATCAATGCG GATGCACAAT CGACGGCTGG TGTGGAACCC 
ACAGGCTATG CGGAGAAAAT CAAGGACTCC TTTGTATGGA AGGATCTGTA CAGTGTGCGA 
AATGTGCACC CTTCTTTCCA CAATCCATTG GGACTGTACG GTGGTCTTGT GCTCAGTGGC 
TTCTCCATTT TCATGGGCGG TCGGGAACCA TGGACCCTAA AACACGGTCC CCAGGATCAC 
GAGTCCTTGA AGCCAGCTAG CTCCTGCCCG CCCATTGTTT ACCCCAAGCC CGATGGCAAG 
ATCTCCTTCG ATCTGCTGTC TTCGGTAGCA CTGACGGGCA CAAACCACGA GGGCGACCAG 
CCCGCCCATC TGACCCTCAA GGACGACCGC ATCCCCGTGG ACCATAATTT GGCGCTGTAC 
GAGGGACCCG AGCAGAGATT CTGCCCCGCT GGCGTTTACG AGTACGTGCC CAACGAGGAG 
GGCGGCAACA TGAAGCTGCA AATTAACGCC CAGAACTGCA TCCACTGCAA GACCTGTGAT 
ATCAAGGACC CCAAGCAGAA CATCAACTGG GTGGTGCCCG AGGGAGGTGG CGGTCCCGCC 
TACAACGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Etf-QO cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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