Etfdh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Etfdh
Entrez Gene ID	295143
Full Name	electron transfer flavoprotein dehydrogenase
General protein information	Preferred Names electron transfer flavoprotein dehydrogenase Names electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial ETF dehydrogenase ETF-QO ETF-ubiquinone oxidoreductase electron transferring flavoprotein dehydrogenase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q33
Summary	human homolog is an iron-sulphur flavoprotein involved in the mitochondrial electron-transfer system; accepts electrons from electron-transfer flavoprotein and in turn reduces ubiquinone [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_198742.2	NP_942037.2	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor
XM_008761084.1	XP_008759306.1	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform X2
XM_006232507.1	XP_006232569.1	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	etfdh	NP_001004598.1
Caenorhabditis elegans (roundworm)	let-721	NP_498415.2
Saccharomyces cerevisiae (baker's yeast)	CIR2	NP_015001.1
Macaca mulatta (Rhesus monkey)	ETFDH	XP_001097120.1
Mus musculus (house mouse)	Etfdh	NP_080070.2
Magnaporthe oryzae (rice blast fungus)	MGG_08880	XP_003713904.1
Gallus gallus (chicken)	ETFDH	NP_001026705.1
Arabidopsis thaliana (thale cress)	ETFQO	NP_181868.1
Xenopus tropicalis (tropical clawed frog)	etfdh	NP_001005631.1
Eremothecium gossypii	AGOS_ADR387W	NP_984483.2
Schizosaccharomyces pombe (fission yeast)	SPAC20G8.04c	NP_593321.1
Kluyveromyces lactis	KLLA0C12991g	XP_452779.1
Rattus norvegicus (Norway rat)	Etfdh	NP_942037.2
Drosophila melanogaster (fruit fly)	Etf-QO	NP_610536.1
Neurospora crassa	NCU04768	XP_960217.1
Oryza sativa (rice)	Os10g0516300	NP_001065062.1
Homo sapiens (human)	ETFDH	NP_004444.2
Canis lupus familiaris (dog)	ETFDH	XP_853781.2
Bos taurus (cattle)	ETFDH	NP_001070598.1
Pan troglodytes (chimpanzee)	ETFDH	XP_517508.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005894	XP_315923.3

Gene Ontology
Bibliography

Related articles in PubMed

Systematic polymorphism discovery after genome-wide identification of potential susceptibility loci in a hereditary rodent model of human hypertension.
Friese RS, Schmid-Schönbein GW, O'Connor DT
Blood pressure20(4)222-31(2011 Aug)

Electron spin relaxation enhancement measurements of interspin distances in human, porcine, and Rhodobacter electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO).
Fielding AJ, Usselman RJ, Watmough N, Simkovic M, Frerman FE, Eaton GR, Eaton SS
Journal of magnetic resonance (San Diego, Calif. : 1997)190(2)222-32(2008 Feb)

The myopathic form of coenzyme Q10 deficiency is caused by mutations in the electron-transferring-flavoprotein dehydrogenase (ETFDH) gene.
Gempel K, Topaloglu H, Talim B, Schneiderat P, Schoser BG, Hans VH, Pálmafy B, Kale G, Tokatli A, Quinzii C, Hirano M, Naini A, DiMauro S, Prokisch H, Lochmüller H, Horvath R
Brain : a journal of neurology130(Pt 8)2037-44(2007 Aug)

Redox proteomic identification of oxidized cardiac proteins in adriamycin-treated mice.
Chen Y, Daosukho C, Opii WO, Turner DM, Pierce WM, Klein JB, Vore M, Butterfield DA, St Clair DK
Free radical biology & medicine41(9)1470-7(2006 Nov)

Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool.
Zhang J, Frerman FE, Kim JJ
Proceedings of the National Academy of Sciences of the United States of America103(44)16212-7(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Etfdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Etfdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12824	NM_198742.2 Latest version!	Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa24787	XM_006232507.1 Latest version!	Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa45280	XM_008761084.1 Latest version!	Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Etfdh ( NM_198742.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_942037.2 Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), mRNA.

Price & Availability↑

CloneID	ORa12824
Clone ID Related Accession (Same CDS sequence)	NM_198742.2
Accession Version	NM_198742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Rattus norvegicus(Norway rat)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081890.1. On Sep 16, 2004 this sequence version replaced NM_198742.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081890.1, FQ211230.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTGGTGC GACTAACCAA GCTGTCCTGC CCTGCATATC AGTGGTTTCA TGCCTTAAAA 
ATTAAAAAGT GCCTACCTCT GTGTGCTCCA AGATGCTCTT CAACCTCTGC TGTACCTCAG 
ATTACCACTC ACTACACTAT TCATCCCCGG GAAAAAGACA AAAGATGGGA AGGAGTGAAT 
ATGGAGAGAT TTGCAGAAGA AGCAGATGTG GTAATAGTTG GTGCCGGCCC AGCAGGGCTC 
TCTGCAGCTA TTCGGCTAAA GCAGCTGGCT GCTGAACAGG AAAAGGACAT CCGTGTGTGT 
CTGGTGGAGA AAGCTGCTCA GATAGGAGCT CATACGCTCT CAGGGGCTTG CCTTGATCCA 
GCTGCTTTTA AAGAGCTCTT CCCAGACTGG AAGGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGGTTTGC GATTTTAACA GAGAAACACA GAATTCCTGT GCCAATTCTT 
CCAGGTCTTC CGATGAACAA TCATGGCAAT TACATCGTAC GCCTCGGACA CCTTGTGAGC 
TGGATGGGAG AACAGGCAGA GGCTCTGGGA GTTGAAGTGT ACCCTGGATA TGCTGCTGCT 
GAGGTCCTTT ATCATGAAGA TGGTAGTGTG AAAGGAATCG CCACTAACGA TGTGGGAATA 
CAGAAGGATG GCGCACCAAA GACAACATTT GAGAGAGGCC TGGAGTTGCA TGCCAAAGTC 
ACAATCTTTG CAGAAGGCTG CCATGGACAC CTAGCCAAGC AGCTTTATAA AAAGTTTGAT 
TTGAGGGCCA GCTGTGATGC CCAGACTTAC GGAATTGGTT TGAAGGAGTT ATGGGTTATT 
GATGAGAAGA AGTGGAAACC TGGGAGAGTA GATCACACTG TTGGCTGGCC CTTGGACAGA 
CATACTTATG GAGGCTCTTT CCTCTATCAC TTAAATGAAG GTGAACCTCT AGTAGCTGTT 
GGTTTTGTGG TTGGCCTGGA CTATCAGAAT CCATACCTGA GTCCATTCAG AGAGTTCCAG 
AGGTGGAAGC ATCACCCCAG CATCCGACCA ACCCTGGAAG GTGGGAAAAG GATAGCCTAT 
GGAGCCCGAG CTCTCAATGA AGGTGGCTTG CAGTCCATAC CAAAACTCAC TTTTCCTGGT 
GGCTTACTAA TTGGTTGCAG TCCTGGATTC ATGAATGTTC CCAAGATCAA AGGTACCCAC 
ACAGCAATGA AAAGTGGAAG CTTGGCAGCA GAAGCAATTT TTAAGCAACT AACTAGTGAA 
AATCTCCAAT CAAAGACAGC AGGACTCCAC GTAACTGAGT ATGAGGACAA TTTGAAGCAA 
TCCTGGGTGT GGAAAGAGCT ACATGCTGTC AGAAATATAA GGCCATCCTG CCACGGGATC 
CTGGGGGTAT ATGGAGGGAT GATTTACACT GGAATATTTT ACTGGATATT GAGAGGAATG 
GAGCCATGGA CTCTAAAACA TAAAGGCTCA GACTCTGAAC AGCTCAAACC AGCCAAGGAC 
TGTACACCCA TTGAGTATCC AAAACCTGAC GGACAGATCA GTTTTGACCT CTTATCCTCT 
GTGGCTCTGA GTGGTACTAA TCATGAACAT GACCAGCCAG CACATTTAAC CTTGAAGGAT 
GACAGCATAC CTGTTAATAG AAATCTGTCA ATATATGATG GGCCTGAGCA GCGATTCTGC 
CCTGCAGGAG TTTATGAATT TGTTCCTCTG GAACAAGGTG ATGGATTTCG GTTACAGATA 
AATGCTCAGA ACTGTGTGCA TTGTAAAACA TGTGATATCA AAGACCCAAG TCAAAATATT 
AACTGGGTGG TCCCAGAAGG TGGAGGAGGA CCTGCTTACA ATGGCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942037.2
CDS	175..2025
Translation	MLVRLTKLSCPAYQWFHALKIKKCLPLCAPRCSSTSAVPQITTHYTIHPREKDKRWEGVNMERFAEEADVVIVGAGPAGLSAAIRLKQLAAEQEKDIRVCLVEKAAQIGAHTLSGACLDPAAFKELFPDWKEKGAPLNTPVTEDRFAILTEKHRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLYHEDGSVKGIATNDVGIQKDGAPKTTFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRASCDAQTYGIGLKELWVIDEKKWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVAVGFVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGLQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGSLAAEAIFKQLTSENLQSKTAGLHVTEYEDNLKQSWVWKELHAVRNIRPSCHGILGVYGGMIYTGIFYWILRGMEPWTLKHKGSDSEQLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLKDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	NM_198742.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198742.2

ORF Insert Sequence:

ATGTTGGTGC GACTAACCAA GCTGTCCTGC CCTGCATATC AGTGGTTTCA TGCCTTAAAA 
ATTAAAAAGT GCCTACCTCT GTGTGCTCCA AGATGCTCTT CAACCTCTGC TGTACCTCAG 
ATTACCACTC ACTACACTAT TCATCCCCGG GAAAAAGACA AAAGATGGGA AGGAGTGAAT 
ATGGAGAGAT TTGCAGAAGA AGCAGATGTG GTAATAGTTG GTGCCGGCCC AGCAGGGCTC 
TCTGCAGCTA TTCGGCTAAA GCAGCTGGCT GCTGAACAGG AAAAGGACAT CCGTGTGTGT 
CTGGTGGAGA AAGCTGCTCA GATAGGAGCT CATACGCTCT CAGGGGCTTG CCTTGATCCA 
GCTGCTTTTA AAGAGCTCTT CCCAGACTGG AAGGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGGTTTGC GATTTTAACA GAGAAACACA GAATTCCTGT GCCAATTCTT 
CCAGGTCTTC CGATGAACAA TCATGGCAAT TACATCGTAC GCCTCGGACA CCTTGTGAGC 
TGGATGGGAG AACAGGCAGA GGCTCTGGGA GTTGAAGTGT ACCCTGGATA TGCTGCTGCT 
GAGGTCCTTT ATCATGAAGA TGGTAGTGTG AAAGGAATCG CCACTAACGA TGTGGGAATA 
CAGAAGGATG GCGCACCAAA GACAACATTT GAGAGAGGCC TGGAGTTGCA TGCCAAAGTC 
ACAATCTTTG CAGAAGGCTG CCATGGACAC CTAGCCAAGC AGCTTTATAA AAAGTTTGAT 
TTGAGGGCCA GCTGTGATGC CCAGACTTAC GGAATTGGTT TGAAGGAGTT ATGGGTTATT 
GATGAGAAGA AGTGGAAACC TGGGAGAGTA GATCACACTG TTGGCTGGCC CTTGGACAGA 
CATACTTATG GAGGCTCTTT CCTCTATCAC TTAAATGAAG GTGAACCTCT AGTAGCTGTT 
GGTTTTGTGG TTGGCCTGGA CTATCAGAAT CCATACCTGA GTCCATTCAG AGAGTTCCAG 
AGGTGGAAGC ATCACCCCAG CATCCGACCA ACCCTGGAAG GTGGGAAAAG GATAGCCTAT 
GGAGCCCGAG CTCTCAATGA AGGTGGCTTG CAGTCCATAC CAAAACTCAC TTTTCCTGGT 
GGCTTACTAA TTGGTTGCAG TCCTGGATTC ATGAATGTTC CCAAGATCAA AGGTACCCAC 
ACAGCAATGA AAAGTGGAAG CTTGGCAGCA GAAGCAATTT TTAAGCAACT AACTAGTGAA 
AATCTCCAAT CAAAGACAGC AGGACTCCAC GTAACTGAGT ATGAGGACAA TTTGAAGCAA 
TCCTGGGTGT GGAAAGAGCT ACATGCTGTC AGAAATATAA GGCCATCCTG CCACGGGATC 
CTGGGGGTAT ATGGAGGGAT GATTTACACT GGAATATTTT ACTGGATATT GAGAGGAATG 
GAGCCATGGA CTCTAAAACA TAAAGGCTCA GACTCTGAAC AGCTCAAACC AGCCAAGGAC 
TGTACACCCA TTGAGTATCC AAAACCTGAC GGACAGATCA GTTTTGACCT CTTATCCTCT 
GTGGCTCTGA GTGGTACTAA TCATGAACAT GACCAGCCAG CACATTTAAC CTTGAAGGAT 
GACAGCATAC CTGTTAATAG AAATCTGTCA ATATATGATG GGCCTGAGCA GCGATTCTGC 
CCTGCAGGAG TTTATGAATT TGTTCCTCTG GAACAAGGTG ATGGATTTCG GTTACAGATA 
AATGCTCAGA ACTGTGTGCA TTGTAAAACA TGTGATATCA AAGACCCAAG TCAAAATATT 
AACTGGGTGG TCCCAGAAGG TGGAGGAGGA CCTGCTTACA ATGGCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Etfdh ( XM_006232507.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232569.1 Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24787
Clone ID Related Accession (Same CDS sequence)	XM_006232507.1
Accession Version	XM_006232507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGC GACTAACCAA GCTGTCCTGC CCTGCATATC AGTGGTTTCA TGCCTTAAAA 
ATTAAAAAGT GCCTACCTCT GTGTGCTCCA AGATGCTCTT CAACCTCTGC TGTACCTCAG 
ATTACCACTC ACTACACTAT TCATCCCCGG GAAAAAGACA AAAGATGGGA AGGAGTGAAT 
ATGGAGAGAT TTGCAGAAGA AGCAGATGTG GTAATAGTTG GTGCCGGCCC AGCAGGGCTC 
TCTGCAGCTA TTCGGCTAAA GCAGCTGGCT GCTGAACAGG AAAAGGACAT CCGTGTGTGT 
CTGGTGGAGA AAGCTGCTCA GATAGGAGCT CATACGCTCT CAGGGGCTTG CCTTGATCCA 
GCTGCTTTTA AAGAGCTCTT CCCAGACTGG AAGGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGGTTTGC GATTTTAACA GAGAAACACA GAATTCCTGT GCCAATTCTT 
CCAGGTCTTC CGATGAACAA TCATGGCAAT TACATCGTAC GCCTCGGACA CCTTGTGAGC 
TGGATGGGAG AACAGGCAGA GGCTCTGGGA GTTGAAGTGT ACCCTGGATA TGCTGCTGCT 
GAGGTCCTTT ATCATGAAGA TGGTAGTGTG AAAGGAATCG CCACTAACGA TGTGGGAATA 
CAGAAGGATG GCGCACCAAA GTTATGGGTT ATTGATGAGA AGAAGTGGAA ACCTGGGAGA 
GTAGATCACA CTGTTGGCTG GCCCTTGGAC AGACATACTT ATGGAGGCTC TTTCCTCTAT 
CACTTAAATG AAGGTGAACC TCTAGTAGCT GTTGGTTTTG TGGTTGGCCT GGACTATCAG 
AATCCATACC TGAGTCCATT CAGAGAGTTC CAGAGGTGGA AGCATCACCC CAGCATCCGA 
CCAACCCTGG AAGGTGGGAA AAGGATAGCC TATGGAGCCC GAGCTCTCAA TGAAGGTGGC 
TTGCAGTCCA TACCAAAACT CACTTTTCCT GGTGGCTTAC TAATTGGTTG CAGTCCTGGA 
TTCATGAATG TTCCCAAGAT CAAAGGTACC CACACAGCAA TGAAAAGTGG AAGCTTGGCA 
GCAGAAGCAA TTTTTAAGCA ACTAACTAGT GAAAATCTCC AATCAAAGAC AGCAGGACTC 
CACGTAACTG AGTATGAGGA CAATTTGAAG CAATCCTGGG TGTGGAAAGA GCTACATGCT 
GTCAGAAATA TAAGGCCATC CTGCCACGGG ATCCTGGGGG TATATGGAGG GATGATTTAC 
ACTGGAATAT TTTACTGGAT ATTGAGAGGA ATGGAGCCAT GGACTCTAAA ACATAAAGGC 
TCAGACTCTG AACAGCTCAA ACCAGCCAAG GACTGTACAC CCATTGAGTA TCCAAAACCT 
GACGGACAGA TCAGTTTTGA CCTCTTATCC TCTGTGGCTC TGAGTGGTAC TAATCATGAA 
CATGACCAGC CAGCACATTT AACCTTGAAG GATGACAGCA TACCTGTTAA TAGAAATCTG 
TCAATATATG ATGGGCCTGA GCAGCGATTC TGCCCTGCAG GAGTTTATGA ATTTGTTCCT 
CTGGAACAAG GTGATGGATT TCGGTTACAG ATAAATGCTC AGAACTGTGT GCATTGTAAA 
ACATGTGATA TCAAAGACCC AAGTCAAAAT ATTAACTGGG TGGTCCCAGA AGGTGGAGGA 
GGACCTGCTT ACAATGGCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232569.1
CDS	175..1878
Translation	MLVRLTKLSCPAYQWFHALKIKKCLPLCAPRCSSTSAVPQITTHYTIHPREKDKRWEGVNMERFAEEADVVIVGAGPAGLSAAIRLKQLAAEQEKDIRVCLVEKAAQIGAHTLSGACLDPAAFKELFPDWKEKGAPLNTPVTEDRFAILTEKHRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLYHEDGSVKGIATNDVGIQKDGAPKLWVIDEKKWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVAVGFVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGLQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGSLAAEAIFKQLTSENLQSKTAGLHVTEYEDNLKQSWVWKELHAVRNIRPSCHGILGVYGGMIYTGIFYWILRGMEPWTLKHKGSDSEQLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLKDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	XM_006232507.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232507.1

ORF Insert Sequence:

ATGTTGGTGC GACTAACCAA GCTGTCCTGC CCTGCATATC AGTGGTTTCA TGCCTTAAAA 
ATTAAAAAGT GCCTACCTCT GTGTGCTCCA AGATGCTCTT CAACCTCTGC TGTACCTCAG 
ATTACCACTC ACTACACTAT TCATCCCCGG GAAAAAGACA AAAGATGGGA AGGAGTGAAT 
ATGGAGAGAT TTGCAGAAGA AGCAGATGTG GTAATAGTTG GTGCCGGCCC AGCAGGGCTC 
TCTGCAGCTA TTCGGCTAAA GCAGCTGGCT GCTGAACAGG AAAAGGACAT CCGTGTGTGT 
CTGGTGGAGA AAGCTGCTCA GATAGGAGCT CATACGCTCT CAGGGGCTTG CCTTGATCCA 
GCTGCTTTTA AAGAGCTCTT CCCAGACTGG AAGGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGGTTTGC GATTTTAACA GAGAAACACA GAATTCCTGT GCCAATTCTT 
CCAGGTCTTC CGATGAACAA TCATGGCAAT TACATCGTAC GCCTCGGACA CCTTGTGAGC 
TGGATGGGAG AACAGGCAGA GGCTCTGGGA GTTGAAGTGT ACCCTGGATA TGCTGCTGCT 
GAGGTCCTTT ATCATGAAGA TGGTAGTGTG AAAGGAATCG CCACTAACGA TGTGGGAATA 
CAGAAGGATG GCGCACCAAA GTTATGGGTT ATTGATGAGA AGAAGTGGAA ACCTGGGAGA 
GTAGATCACA CTGTTGGCTG GCCCTTGGAC AGACATACTT ATGGAGGCTC TTTCCTCTAT 
CACTTAAATG AAGGTGAACC TCTAGTAGCT GTTGGTTTTG TGGTTGGCCT GGACTATCAG 
AATCCATACC TGAGTCCATT CAGAGAGTTC CAGAGGTGGA AGCATCACCC CAGCATCCGA 
CCAACCCTGG AAGGTGGGAA AAGGATAGCC TATGGAGCCC GAGCTCTCAA TGAAGGTGGC 
TTGCAGTCCA TACCAAAACT CACTTTTCCT GGTGGCTTAC TAATTGGTTG CAGTCCTGGA 
TTCATGAATG TTCCCAAGAT CAAAGGTACC CACACAGCAA TGAAAAGTGG AAGCTTGGCA 
GCAGAAGCAA TTTTTAAGCA ACTAACTAGT GAAAATCTCC AATCAAAGAC AGCAGGACTC 
CACGTAACTG AGTATGAGGA CAATTTGAAG CAATCCTGGG TGTGGAAAGA GCTACATGCT 
GTCAGAAATA TAAGGCCATC CTGCCACGGG ATCCTGGGGG TATATGGAGG GATGATTTAC 
ACTGGAATAT TTTACTGGAT ATTGAGAGGA ATGGAGCCAT GGACTCTAAA ACATAAAGGC 
TCAGACTCTG AACAGCTCAA ACCAGCCAAG GACTGTACAC CCATTGAGTA TCCAAAACCT 
GACGGACAGA TCAGTTTTGA CCTCTTATCC TCTGTGGCTC TGAGTGGTAC TAATCATGAA 
CATGACCAGC CAGCACATTT AACCTTGAAG GATGACAGCA TACCTGTTAA TAGAAATCTG 
TCAATATATG ATGGGCCTGA GCAGCGATTC TGCCCTGCAG GAGTTTATGA ATTTGTTCCT 
CTGGAACAAG GTGATGGATT TCGGTTACAG ATAAATGCTC AGAACTGTGT GCATTGTAAA 
ACATGTGATA TCAAAGACCC AAGTCAAAAT ATTAACTGGG TGGTCCCAGA AGGTGGAGGA 
GGACCTGCTT ACAATGGCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Etfdh ( XM_008761084.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759306.1 Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa45280
Clone ID Related Accession (Same CDS sequence)	XM_008761084.1
Accession Version	XM_008761084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAT TTGCAGAAGA AGCAGATGTG GTAATAGTTG GTGCCGGCCC AGCAGGGCTC 
TCTGCAGCTA TTCGGCTAAA GCAGCTGGCT GCTGAACAGG AAAAGGACAT CCGTGTGTGT 
CTGGTGGAGA AAGCTGCTCA GATAGGAGCT CATACGCTCT CAGGGGCTTG CCTTGATCCA 
GCTGCTTTTA AAGAGCTCTT CCCAGACTGG AAGGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGGTTTGC GATTTTAACA GAGAAACACA GAATTCCTGT GCCAATTCTT 
CCAGGTCTTC CGATGAACAA TCATGGCAAT TACATCGTAC GCCTCGGACA CCTTGTGAGC 
TGGATGGGAG AACAGGCAGA GGCTCTGGGA GTTGAAGTGT ACCCTGGATA TGCTGCTGCT 
GAGGTCCTTT ATCATGAAGA TGGTAGTGTG AAAGGAATCG CCACTAACGA TGTGGGAATA 
CAGAAGGATG GCGCACCAAA GACAACATTT GAGAGAGGCC TGGAGTTGCA TGCCAAAGTC 
ACAATCTTTG CAGAAGGCTG CCATGGACAC CTAGCCAAGC AGCTTTATAA AAAGTTTGAT 
TTGAGGGCCA GCTGTGATGC CCAGACTTAC GGAATTGGTT TGAAGGAGTT ATGGGTTATT 
GATGAGAAGA AGTGGAAACC TGGGAGAGTA GATCACACTG TTGGCTGGCC CTTGGACAGA 
CATACTTATG GAGGCTCTTT CCTCTATCAC TTAAATGAAG GTGAACCTCT AGTAGCTGTT 
GGTTTTGTGG TTGGCCTGGA CTATCAGAAT CCATACCTGA GTCCATTCAG AGAGTTCCAG 
AGGTGGAAGC ATCACCCCAG CATCCGACCA ACCCTGGAAG GTGGGAAAAG GATAGCCTAT 
GGAGCCCGAG CTCTCAATGA AGGTGGCTTG CAGTCCATAC CAAAACTCAC TTTTCCTGGT 
GGCTTACTAA TTGGTTGCAG TCCTGGATTC ATGAATGTTC CCAAGATCAA AGGTACCCAC 
ACAGCAATGA AAAGTGGAAG CTTGGCAGCA GAAGCAATTT TTAAGCAACT AACTAGTGAA 
AATCTCCAAT CAAAGACAGC AGGACTCCAC GTAACTGAGT ATGAGGACAA TTTGAAGCAA 
TCCTGGGTGT GGAAAGAGCT ACATGCTGTC AGAAATATAA GGCCATCCTG CCACGGGATC 
CTGGGGGTAT ATGGAGGGAT GATTTACACT GGAATATTTT ACTGGATATT GAGAGGAATG 
GAGCCATGGA CTCTAAAACA TAAAGGCTCA GACTCTGAAC AGCTCAAACC AGCCAAGGAC 
TGTACACCCA TTGAGTATCC AAAACCTGAC GGACAGATCA GTTTTGACCT CTTATCCTCT 
GTGGCTCTGA GTGGTACTAA TCATGAACAT GACCAGCCAG CACATTTAAC CTTGAAGGAT 
GACAGCATAC CTGTTAATAG AAATCTGTCA ATATATGATG GGCCTGAGCA GCGATTCTGC 
CCTGCAGGAG TTTATGAATT TGTTCCTCTG GAACAAGGTG ATGGATTTCG GTTACAGATA 
AATGCTCAGA ACTGTGTGCA TTGTAAAACA TGTGATATCA AAGACCCAAG TCAAAATATT 
AACTGGGTGG TCCCAGAAGG TGGAGGAGGA CCTGCTTACA ATGGCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759306.1
CDS	581..2251
Translation	MERFAEEADVVIVGAGPAGLSAAIRLKQLAAEQEKDIRVCLVEKAAQIGAHTLSGACLDPAAFKELFPDWKEKGAPLNTPVTEDRFAILTEKHRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLYHEDGSVKGIATNDVGIQKDGAPKTTFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRASCDAQTYGIGLKELWVIDEKKWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVAVGFVVGLDYQNPYLSPFREFQRWKHHPSIRPTLEGGKRIAYGARALNEGGLQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGSLAAEAIFKQLTSENLQSKTAGLHVTEYEDNLKQSWVWKELHAVRNIRPSCHGILGVYGGMIYTGIFYWILRGMEPWTLKHKGSDSEQLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLKDDSIPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

Target ORF information:

RefSeq Version	XM_008761084.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus electron transfer flavoprotein dehydrogenase (Etfdh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761084.1

ORF Insert Sequence:

ATGGAGAGAT TTGCAGAAGA AGCAGATGTG GTAATAGTTG GTGCCGGCCC AGCAGGGCTC 
TCTGCAGCTA TTCGGCTAAA GCAGCTGGCT GCTGAACAGG AAAAGGACAT CCGTGTGTGT 
CTGGTGGAGA AAGCTGCTCA GATAGGAGCT CATACGCTCT CAGGGGCTTG CCTTGATCCA 
GCTGCTTTTA AAGAGCTCTT CCCAGACTGG AAGGAGAAGG GGGCTCCACT TAACACTCCT 
GTAACAGAAG ACAGGTTTGC GATTTTAACA GAGAAACACA GAATTCCTGT GCCAATTCTT 
CCAGGTCTTC CGATGAACAA TCATGGCAAT TACATCGTAC GCCTCGGACA CCTTGTGAGC 
TGGATGGGAG AACAGGCAGA GGCTCTGGGA GTTGAAGTGT ACCCTGGATA TGCTGCTGCT 
GAGGTCCTTT ATCATGAAGA TGGTAGTGTG AAAGGAATCG CCACTAACGA TGTGGGAATA 
CAGAAGGATG GCGCACCAAA GACAACATTT GAGAGAGGCC TGGAGTTGCA TGCCAAAGTC 
ACAATCTTTG CAGAAGGCTG CCATGGACAC CTAGCCAAGC AGCTTTATAA AAAGTTTGAT 
TTGAGGGCCA GCTGTGATGC CCAGACTTAC GGAATTGGTT TGAAGGAGTT ATGGGTTATT 
GATGAGAAGA AGTGGAAACC TGGGAGAGTA GATCACACTG TTGGCTGGCC CTTGGACAGA 
CATACTTATG GAGGCTCTTT CCTCTATCAC TTAAATGAAG GTGAACCTCT AGTAGCTGTT 
GGTTTTGTGG TTGGCCTGGA CTATCAGAAT CCATACCTGA GTCCATTCAG AGAGTTCCAG 
AGGTGGAAGC ATCACCCCAG CATCCGACCA ACCCTGGAAG GTGGGAAAAG GATAGCCTAT 
GGAGCCCGAG CTCTCAATGA AGGTGGCTTG CAGTCCATAC CAAAACTCAC TTTTCCTGGT 
GGCTTACTAA TTGGTTGCAG TCCTGGATTC ATGAATGTTC CCAAGATCAA AGGTACCCAC 
ACAGCAATGA AAAGTGGAAG CTTGGCAGCA GAAGCAATTT TTAAGCAACT AACTAGTGAA 
AATCTCCAAT CAAAGACAGC AGGACTCCAC GTAACTGAGT ATGAGGACAA TTTGAAGCAA 
TCCTGGGTGT GGAAAGAGCT ACATGCTGTC AGAAATATAA GGCCATCCTG CCACGGGATC 
CTGGGGGTAT ATGGAGGGAT GATTTACACT GGAATATTTT ACTGGATATT GAGAGGAATG 
GAGCCATGGA CTCTAAAACA TAAAGGCTCA GACTCTGAAC AGCTCAAACC AGCCAAGGAC 
TGTACACCCA TTGAGTATCC AAAACCTGAC GGACAGATCA GTTTTGACCT CTTATCCTCT 
GTGGCTCTGA GTGGTACTAA TCATGAACAT GACCAGCCAG CACATTTAAC CTTGAAGGAT 
GACAGCATAC CTGTTAATAG AAATCTGTCA ATATATGATG GGCCTGAGCA GCGATTCTGC 
CCTGCAGGAG TTTATGAATT TGTTCCTCTG GAACAAGGTG ATGGATTTCG GTTACAGATA 
AATGCTCAGA ACTGTGTGCA TTGTAAAACA TGTGATATCA AAGACCCAAG TCAAAATATT 
AACTGGGTGG TCCCAGAAGG TGGAGGAGGA CCTGCTTACA ATGGCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic polymorphism discovery after genome-wide identification of potential susceptibility loci in a hereditary rodent model of human hypertension.
Blood pressure20(4)222-31(2011 Aug)
Friese RS,Schmid-Schönbein GW,O'Connor DT

Electron spin relaxation enhancement measurements of interspin distances in human, porcine, and Rhodobacter electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO).
Journal of magnetic resonance (San Diego, Calif. : 1997)190(2)222-32(2008 Feb)
Fielding AJ,Usselman RJ,Watmough N,Simkovic M,Frerman FE,Eaton GR,Eaton SS

The myopathic form of coenzyme Q10 deficiency is caused by mutations in the electron-transferring-flavoprotein dehydrogenase (ETFDH) gene.
Brain : a journal of neurology130(Pt 8)2037-44(2007 Aug)
Gempel K,Topaloglu H,Talim B,Schneiderat P,Schoser BG,Hans VH,Pálmafy B,Kale G,Tokatli A,Quinzii C,Hirano M,Naini A,DiMauro S,Prokisch H,Lochmüller H,Horvath R

Redox proteomic identification of oxidized cardiac proteins in adriamycin-treated mice.
Free radical biology & medicine41(9)1470-7(2006 Nov)
Chen Y,Daosukho C,Opii WO,Turner DM,Pierce WM,Klein JB,Vore M,Butterfield DA,St Clair DK

Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool.
Proceedings of the National Academy of Sciences of the United States of America103(44)16212-7(2006 Oct)
Zhang J,Frerman FE,Kim JJ

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Etfdh cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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