RfC38 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RfC38
Entrez Gene ID	34550
Full Name	Replication factor C 38kD subunit
Synonyms	BcDNA:LD06837,CG6258,DmRFC5,Dmel\CG6258,Rfc38,l(2)k13807,l(2)rfc38,n(2)k13807
General protein information	Preferred Names Replication factor C 38kD subunit Names CG6258 gene product from transcript CG6258-RB CG6258-PA CG6258-PB DNA replication factor, 38k subunit RfC38-PA RfC38-PB replication factor C 38kD subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32D4-32D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298904.1	NP_001285833.1	replication factor C 38kD subunit, isoform B
NM_135650.3	NP_609494.1	replication factor C 38kD subunit, isoform A

Pathways from BioSystems

Homologies

Neurospora crassa	NCU06769	XP_963541.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009047	XP_319799.4
Eremothecium gossypii	AGOS_ADL303C	NP_983793.1
Rattus norvegicus (Norway rat)	Rfc3	NP_001009629.1
Arabidopsis thaliana (thale cress)	EMB2775	NP_198126.1
Magnaporthe oryzae (rice blast fungus)	MGG_03202	XP_003716781.1
Homo sapiens (human)	RFC3	NP_002906.1
Canis lupus familiaris (dog)	RFC3	XP_534500.3
Mus musculus (house mouse)	Rfc3	NP_081285.1
Saccharomyces cerevisiae (baker's yeast)	RFC5	NP_009644.3
Oryza sativa (rice)	Os03g0792600	NP_001051525.1
Bos taurus (cattle)	RFC3	NP_001033636.1
Danio rerio (zebrafish)	rfc3	NP_958865.1
Drosophila melanogaster (fruit fly)	RfC38	NP_609494.1
Kluyveromyces lactis	KLLA0B12221g	XP_452074.1
Pan troglodytes (chimpanzee)	RFC3	XP_509625.2
Macaca mulatta (Rhesus monkey)	RFC3	XP_001102503.1
Gallus gallus (chicken)	RFC3	NP_001006276.1
Schizosaccharomyces pombe (fission yeast)	rfc5	NP_595646.1
Xenopus tropicalis (tropical clawed frog)	rfc3	XP_002934079.1
Caenorhabditis elegans (roundworm)	rfc-3	NP_502517.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RfC38 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RfC38 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16663	NM_001298904.1 Latest version!	Drosophila melanogaster replication factor C 38kD subunit, transcript variant B (RfC38), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa16663	NM_135650.3 Latest version!	Drosophila melanogaster replication factor C 38kD subunit, transcript variant A (RfC38), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RfC38 ( NM_001298904.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285833.1 Drosophila melanogaster replication factor C 38kD subunit, transcript variant B (RfC38), mRNA.

Price & Availability↑

CloneID	OFa16663
Clone ID Related Accession (Same CDS sequence)	NM_001298904.1 , NM_135650.3
Accession Version	NM_001298904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	replication factor C 38kD subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACTTT GGGTGGATAA ATACCGTCCG CGGGAGCTGT CCAAATTGGA CTTCCACAAG 
GATCAAGCGG AGAACCTACG CAACCTTTGC AAGCAGAGCG ATTTTCCTCA TCTGATGTTC 
TACGGACCCT CGGGTGCTGG CAAGAAGACA CGCATCATGT GCCTGCTACG GGAGATGTAC 
GGATCCGGCG TTGAGCGGTT GAGGAGCGAG ACCATGACAT TCACCACGCC ATCCAATCGG 
AAGGTAGAGG TGATGACGGT CAGCAGCAAC TACCACTTGG AGGTGAATCC CTCCGACGCT 
GGCATGTACG ACCGCACGGT GGTGATCGAT CTCATCAAGC AGGTGGCCCA GACGCATCAA 
ATTGAGATTA GTGGCCAACG GGAATTCAAG GTGATTGTAA TTTCGGAGGG CGACGAACTC 
ACCAAGGATG CCCAGCACGC CCTGCGCCGT ACGATGGAGA AATATGTGGC CACGTGCCGG 
ATAATCATAT CGGTGAACTC CACATCTCGG ATCATTCCGG CCATACGGTC GCGTTGCCTG 
GGCATACGGG TGGCGGCTCC CAACGAAACG GAGATCGTGT CCATCTTGCA GAACACTTGC 
AAGCGGGAGG GACTCGCACT GCCCGTGGAG CTGGCCAAAC GGGTTGTGGA CAAGTCGGAG 
CGCAATCTCA GGCGAGCACT TTTGATGCTG GAGGCCGCCA AGGTGGCCAA GGCGCCGTTT 
ACCGCCAATC AGGAGATTCC CGACCTGGAC TGGCAGGTGT TCCTGCGTGA GACAGCATCT 
CAGATTATCA GTGAGCAAAC GCCTGCCAAA CTGGAGAAGA TCCGCGAACG CCTGTACGAG 
CTACTCACGC AAGGCGTTCC ACCTAATCTC ATATTCCGCG GCCTGGTCGA ACAGCTGGTT 
AACAATTGTG ATATGTCCAT CAAGGCAAAG ACGCTCGAGT TCGCCACCGA GTACGAGCAC 
CGGATGCAGT CCGGCGCCAA GCACATTTTT CATCTTGAAG CGTTCGTCGC TCAGTTTATG 
AACATCTATA AAAAGTTCCT TTCTGAGCTG GACATGACGG ATGATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285833.1
CDS	475..1545
Translation	MALWVDKYRPRELSKLDFHKDQAENLRNLCKQSDFPHLMFYGPSGAGKKTRIMCLLREMYGSGVERLRSETMTFTTPSNRKVEVMTVSSNYHLEVNPSDAGMYDRTVVIDLIKQVAQTHQIEISGQREFKVIVISEGDELTKDAQHALRRTMEKYVATCRIIISVNSTSRIIPAIRSRCLGIRVAAPNETEIVSILQNTCKREGLALPVELAKRVVDKSERNLRRALLMLEAAKVAKAPFTANQEIPDLDWQVFLRETASQIISEQTPAKLEKIRERLYELLTQGVPPNLIFRGLVEQLVNNCDMSIKAKTLEFATEYEHRMQSGAKHIFHLEAFVAQFMNIYKKFLSELDMTDDF

Target ORF information:

RefSeq Version	NM_001298904.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster replication factor C 38kD subunit, transcript variant B (RfC38), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298904.1

ORF Insert Sequence:

ATGGCACTTT GGGTGGATAA ATACCGTCCG CGGGAGCTGT CCAAATTGGA CTTCCACAAG 
GATCAAGCGG AGAACCTACG CAACCTTTGC AAGCAGAGCG ATTTTCCTCA TCTGATGTTC 
TACGGACCCT CGGGTGCTGG CAAGAAGACA CGCATCATGT GCCTGCTACG GGAGATGTAC 
GGATCCGGCG TTGAGCGGTT GAGGAGCGAG ACCATGACAT TCACCACGCC ATCCAATCGG 
AAGGTAGAGG TGATGACGGT CAGCAGCAAC TACCACTTGG AGGTGAATCC CTCCGACGCT 
GGCATGTACG ACCGCACGGT GGTGATCGAT CTCATCAAGC AGGTGGCCCA GACGCATCAA 
ATTGAGATTA GTGGCCAACG GGAATTCAAG GTGATTGTAA TTTCGGAGGG CGACGAACTC 
ACCAAGGATG CCCAGCACGC CCTGCGCCGT ACGATGGAGA AATATGTGGC CACGTGCCGG 
ATAATCATAT CGGTGAACTC CACATCTCGG ATCATTCCGG CCATACGGTC GCGTTGCCTG 
GGCATACGGG TGGCGGCTCC CAACGAAACG GAGATCGTGT CCATCTTGCA GAACACTTGC 
AAGCGGGAGG GACTCGCACT GCCCGTGGAG CTGGCCAAAC GGGTTGTGGA CAAGTCGGAG 
CGCAATCTCA GGCGAGCACT TTTGATGCTG GAGGCCGCCA AGGTGGCCAA GGCGCCGTTT 
ACCGCCAATC AGGAGATTCC CGACCTGGAC TGGCAGGTGT TCCTGCGTGA GACAGCATCT 
CAGATTATCA GTGAGCAAAC GCCTGCCAAA CTGGAGAAGA TCCGCGAACG CCTGTACGAG 
CTACTCACGC AAGGCGTTCC ACCTAATCTC ATATTCCGCG GCCTGGTCGA ACAGCTGGTT 
AACAATTGTG ATATGTCCAT CAAGGCAAAG ACGCTCGAGT TCGCCACCGA GTACGAGCAC 
CGGATGCAGT CCGGCGCCAA GCACATTTTT CATCTTGAAG CGTTCGTCGC TCAGTTTATG 
AACATCTATA AAAAGTTCCT TTCTGAGCTG GACATGACGG ATGATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RfC38 ( NM_135650.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609494.1 Drosophila melanogaster replication factor C 38kD subunit, transcript variant A (RfC38), mRNA.

Price & Availability↑

CloneID	OFa16663
Clone ID Related Accession (Same CDS sequence)	NM_001298904.1 , NM_135650.3
Accession Version	NM_135650.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	replication factor C 38kD subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135650.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACTTT GGGTGGATAA ATACCGTCCG CGGGAGCTGT CCAAATTGGA CTTCCACAAG 
GATCAAGCGG AGAACCTACG CAACCTTTGC AAGCAGAGCG ATTTTCCTCA TCTGATGTTC 
TACGGACCCT CGGGTGCTGG CAAGAAGACA CGCATCATGT GCCTGCTACG GGAGATGTAC 
GGATCCGGCG TTGAGCGGTT GAGGAGCGAG ACCATGACAT TCACCACGCC ATCCAATCGG 
AAGGTAGAGG TGATGACGGT CAGCAGCAAC TACCACTTGG AGGTGAATCC CTCCGACGCT 
GGCATGTACG ACCGCACGGT GGTGATCGAT CTCATCAAGC AGGTGGCCCA GACGCATCAA 
ATTGAGATTA GTGGCCAACG GGAATTCAAG GTGATTGTAA TTTCGGAGGG CGACGAACTC 
ACCAAGGATG CCCAGCACGC CCTGCGCCGT ACGATGGAGA AATATGTGGC CACGTGCCGG 
ATAATCATAT CGGTGAACTC CACATCTCGG ATCATTCCGG CCATACGGTC GCGTTGCCTG 
GGCATACGGG TGGCGGCTCC CAACGAAACG GAGATCGTGT CCATCTTGCA GAACACTTGC 
AAGCGGGAGG GACTCGCACT GCCCGTGGAG CTGGCCAAAC GGGTTGTGGA CAAGTCGGAG 
CGCAATCTCA GGCGAGCACT TTTGATGCTG GAGGCCGCCA AGGTGGCCAA GGCGCCGTTT 
ACCGCCAATC AGGAGATTCC CGACCTGGAC TGGCAGGTGT TCCTGCGTGA GACAGCATCT 
CAGATTATCA GTGAGCAAAC GCCTGCCAAA CTGGAGAAGA TCCGCGAACG CCTGTACGAG 
CTACTCACGC AAGGCGTTCC ACCTAATCTC ATATTCCGCG GCCTGGTCGA ACAGCTGGTT 
AACAATTGTG ATATGTCCAT CAAGGCAAAG ACGCTCGAGT TCGCCACCGA GTACGAGCAC 
CGGATGCAGT CCGGCGCCAA GCACATTTTT CATCTTGAAG CGTTCGTCGC TCAGTTTATG 
AACATCTATA AAAAGTTCCT TTCTGAGCTG GACATGACGG ATGATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609494.1
CDS	147..1217
Translation	MALWVDKYRPRELSKLDFHKDQAENLRNLCKQSDFPHLMFYGPSGAGKKTRIMCLLREMYGSGVERLRSETMTFTTPSNRKVEVMTVSSNYHLEVNPSDAGMYDRTVVIDLIKQVAQTHQIEISGQREFKVIVISEGDELTKDAQHALRRTMEKYVATCRIIISVNSTSRIIPAIRSRCLGIRVAAPNETEIVSILQNTCKREGLALPVELAKRVVDKSERNLRRALLMLEAAKVAKAPFTANQEIPDLDWQVFLRETASQIISEQTPAKLEKIRERLYELLTQGVPPNLIFRGLVEQLVNNCDMSIKAKTLEFATEYEHRMQSGAKHIFHLEAFVAQFMNIYKKFLSELDMTDDF

Target ORF information:

RefSeq Version	NM_135650.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster replication factor C 38kD subunit, transcript variant A (RfC38), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135650.3

ORF Insert Sequence:

ATGGCACTTT GGGTGGATAA ATACCGTCCG CGGGAGCTGT CCAAATTGGA CTTCCACAAG 
GATCAAGCGG AGAACCTACG CAACCTTTGC AAGCAGAGCG ATTTTCCTCA TCTGATGTTC 
TACGGACCCT CGGGTGCTGG CAAGAAGACA CGCATCATGT GCCTGCTACG GGAGATGTAC 
GGATCCGGCG TTGAGCGGTT GAGGAGCGAG ACCATGACAT TCACCACGCC ATCCAATCGG 
AAGGTAGAGG TGATGACGGT CAGCAGCAAC TACCACTTGG AGGTGAATCC CTCCGACGCT 
GGCATGTACG ACCGCACGGT GGTGATCGAT CTCATCAAGC AGGTGGCCCA GACGCATCAA 
ATTGAGATTA GTGGCCAACG GGAATTCAAG GTGATTGTAA TTTCGGAGGG CGACGAACTC 
ACCAAGGATG CCCAGCACGC CCTGCGCCGT ACGATGGAGA AATATGTGGC CACGTGCCGG 
ATAATCATAT CGGTGAACTC CACATCTCGG ATCATTCCGG CCATACGGTC GCGTTGCCTG 
GGCATACGGG TGGCGGCTCC CAACGAAACG GAGATCGTGT CCATCTTGCA GAACACTTGC 
AAGCGGGAGG GACTCGCACT GCCCGTGGAG CTGGCCAAAC GGGTTGTGGA CAAGTCGGAG 
CGCAATCTCA GGCGAGCACT TTTGATGCTG GAGGCCGCCA AGGTGGCCAA GGCGCCGTTT 
ACCGCCAATC AGGAGATTCC CGACCTGGAC TGGCAGGTGT TCCTGCGTGA GACAGCATCT 
CAGATTATCA GTGAGCAAAC GCCTGCCAAA CTGGAGAAGA TCCGCGAACG CCTGTACGAG 
CTACTCACGC AAGGCGTTCC ACCTAATCTC ATATTCCGCG GCCTGGTCGA ACAGCTGGTT 
AACAATTGTG ATATGTCCAT CAAGGCAAAG ACGCTCGAGT TCGCCACCGA GTACGAGCAC 
CGGATGCAGT CCGGCGCCAA GCACATTTTT CATCTTGAAG CGTTCGTCGC TCAGTTTATG 
AACATCTATA AAAAGTTCCT TTCTGAGCTG GACATGACGG ATGATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RfC38 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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