Gprk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gprk1
Entrez Gene ID	3355013
Full Name	G protein-coupled receptor kinase 1
Synonyms	CG40129,CT4320,Dmel\CG40129,GPRK1,Gprk-1,RK,Rok,gprk1
General protein information	Preferred Names G protein-coupled receptor kinase 1 Names CG40129 gene product from transcript CG40129-RC CG40129-PA CG40129-PB CG40129-PC Gprk1-PA Gprk1-PB Gprk1-PC rhodopsin kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 41B3-41C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299206.1	NP_001286135.1	G protein-coupled receptor kinase 1, isoform C
NM_001169580.2	NP_001163051.1	G protein-coupled receptor kinase 1, isoform B
NM_001042973.3	NP_001036438.1	G protein-coupled receptor kinase 1, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	ADRBK2	NP_005151.2
Caenorhabditis elegans (roundworm)	grk-2	NP_497235.2
Mus musculus (house mouse)	Adrbk2	NP_796052.2
Pan troglodytes (chimpanzee)	ADRBK2	XP_003317197.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012026	XP_320502.4
Drosophila melanogaster (fruit fly)	Gprk1	NP_001163051.1
Canis lupus familiaris (dog)	ADRBK2	XP_543453.3
Xenopus tropicalis (tropical clawed frog)	adrbk2	XP_002932117.2
Macaca mulatta (Rhesus monkey)	ADRBK2	XP_001104308.1
Bos taurus (cattle)	ADRBK2	NP_776925.1
Rattus norvegicus (Norway rat)	Adrbk2	NP_037029.1
Gallus gallus (chicken)	ADRBK2	XP_415195.4
Danio rerio (zebrafish)	adrbk2	NP_001128197.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gprk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gprk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11822	NM_001169580.2 Latest version!	Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa11822	NM_001299206.1 Latest version!	Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa11822	NM_001042973.3 Latest version!	Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gprk1 ( NM_001299206.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286135.1 Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11822
Clone ID Related Accession (Same CDS sequence)	NM_001299206.1 , NM_001169580.2 , NM_001042973.3
Accession Version	NM_001299206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	G protein-coupled receptor kinase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGATT TGGAAGCAGT CTTGGCTGAT GTCAGCTATT TAATGGCAAT GGAAAAGTCT 
AAATGTACTC CAGCTGCGAG GGCAAGCAAG AAATTAAATT TACCCGACCC GAGTGTTAGA 
AGTGTTATGT ATAAATATTT GGAAAAAGAA GGCGAATTGA ATTTTCATAA AATATTTAAC 
GAAGTTTTGG GCTATTTGTT GTTTAAAGAC TTTTGTGAAA ACGACTCAGA AGAACCTATA 
CAGCAACTTA AGTTTTTTGA ACAGATAAAA CTCTTTGAAA AAACAGAATG TTATGATGAG 
CGGAAAAAAA TGGCAAGGGA TATTTATGAC AATTTTATAA TGGAGGAAAT GTTGTCCCAC 
ACCTATGAAT ACTCCAAACA TGCTGTTGCG AGTGTACAAA AATATTTATT AAAAAATGAG 
GTCCCGGTTG ACTTATTTGA ACCATACTTG GAAGAAATAT TTACACAACT TAAAGGAAAG 
CCATTTAAAA AATTTTTAGA AAGCGATAAA TTTACTAGGT TTTGCCAGTG GAAAAATTTA 
GAACTGAACA TACAACTAAC TATGAACGAT TTCAGCGTTC ATAGAATAAT TGGACGGGGG 
GGTTTCGGAG AAGTATATGG ATGCCGAAAA GCTGACACTG GAAAAATGTA TGCTATGAAA 
TGCTTGGACA AAAAACGGAT TAAAATGAAA CAAGGAGAAA TGCTAGCTCT TAATGAAAGA 
AATATGCTGC AAGCAGTCAG TACTGGAATT GATTGTCCGT TCATAGTGTG TATGACGTAT 
GCCTTTCATA CGCCGGATAA GCTATGTTTT ATATTAGATC TAATGAATGG TGGTGATCTA 
CATTACCATT TATCTCAACA TGGTATTTTC AGTGAGGATG AAATGAAATT TTATGCAGCG 
GAGGTGATTT TGGGATTGGA ACACATGCAT AAACGTTGCA TTGTGTACCG AGACTTAAAA 
CCCGCCAACA TACTGCTCGA TGAAAACGGA CACATACGAA TTTCTGATTT GGGATTGGCT 
TGCGACTTCT CAAAAAAGAA ACCGCATGCT TCAGTGGGAA CTCATGGATA CATGGCTCCA 
GAGGTGCTAT CTAAAGGAAC ATCATACGAT TCGTGCGCAG ATTGGTTTAG CTTCGGATGC 
ATGTTATATA AATTGCTTAA AGGTCATTCT CCATTTCGAC AGCATAAAAC AAAGGATAAG 
CTAGAAATTG ACAAAATGAC ATTAACTATG AACGTAGAAC TTCCAGAATC GTTTTCTTTG 
GAACTAAAGA ATTTGTTGGA AATGTTACTT CAAAGGGACG TCTCTAAAAG GCTTGGGTGC 
ATGGGAAATG GCGCGGATGA AGTAAAAATG CACAATTTTT TCTGTGGAAT TGATTGGCAC 
CAGGTTTACA TACAAAAATA TACTCCACCT TTAGTACCCC CTAGAGGGGA AGTAAATGCC 
GCAGATGCCT TCGATATTGG ATCATTTGAT GAGGAAGATA CCAAGGGCAT AAAACTAAAC 
GACGCCGATC AAGATCTATA TAAAATGTTT TCGTTGACAA TATCTGAAAG GTGGCAACAG 
GAAGTTTCTG AAACAGTATT TGATACAGTC AACACTGAGA CAGATAAACT AGAACAAAAA 
AGAAAATTGA AGCAAAAGCA GCACTTCGAT GCAGATGAAA AGGAATCCGA TTGTATATTA 
CATGGCTACA TAAAAAAGCT TGGGGGCTCT TTTGCATCTT TATGGCAAAC AAAATATGCC 
AAGCTATATC CAAATAGGCT GGAGCTACAT TCGGAAAGTG GAAATAATAA ACCAGAACTA 
ATTTTTATGG ACCAAGTAGA AGATATATCA TCGGATTTCA TTCTGCACAA AAACGAGAAT 
TGCATTCAAA TTCGCATTAA TGATGGAACC CGTGATGGCA GAATCATTTT GACAAACTCA 
GATGAAATAG GATTAAAAGA ATGGTCATCT TCCCTAAGAT CGGCACACAA AATTTCTCAA 
GACCTCTTGG GGTCAATGGC TAAAAAAGCT GGAAAAATAT ATGGCAGTGA AAGGGATGTC 
AACAAATCTA TGTATATTTT TGGTGGGAAC TGCTCAACGA AAACTTCTAA CGGGTCTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286135.1
CDS	179..2281
Translation	MADLEAVLADVSYLMAMEKSKCTPAARASKKLNLPDPSVRSVMYKYLEKEGELNFHKIFNEVLGYLLFKDFCENDSEEPIQQLKFFEQIKLFEKTECYDERKKMARDIYDNFIMEEMLSHTYEYSKHAVASVQKYLLKNEVPVDLFEPYLEEIFTQLKGKPFKKFLESDKFTRFCQWKNLELNIQLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGEMLALNERNMLQAVSTGIDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGIFSEDEMKFYAAEVILGLEHMHKRCIVYRDLKPANILLDENGHIRISDLGLACDFSKKKPHASVGTHGYMAPEVLSKGTSYDSCADWFSFGCMLYKLLKGHSPFRQHKTKDKLEIDKMTLTMNVELPESFSLELKNLLEMLLQRDVSKRLGCMGNGADEVKMHNFFCGIDWHQVYIQKYTPPLVPPRGEVNAADAFDIGSFDEEDTKGIKLNDADQDLYKMFSLTISERWQQEVSETVFDTVNTETDKLEQKRKLKQKQHFDADEKESDCILHGYIKKLGGSFASLWQTKYAKLYPNRLELHSESGNNKPELIFMDQVEDISSDFILHKNENCIQIRINDGTRDGRIILTNSDEIGLKEWSSSLRSAHKISQDLLGSMAKKAGKIYGSERDVNKSMYIFGGNCSTKTSNGSN

Target ORF information:

RefSeq Version	NM_001299206.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299206.1

ORF Insert Sequence:

ATGGCTGATT TGGAAGCAGT CTTGGCTGAT GTCAGCTATT TAATGGCAAT GGAAAAGTCT 
AAATGTACTC CAGCTGCGAG GGCAAGCAAG AAATTAAATT TACCCGACCC GAGTGTTAGA 
AGTGTTATGT ATAAATATTT GGAAAAAGAA GGCGAATTGA ATTTTCATAA AATATTTAAC 
GAAGTTTTGG GCTATTTGTT GTTTAAAGAC TTTTGTGAAA ACGACTCAGA AGAACCTATA 
CAGCAACTTA AGTTTTTTGA ACAGATAAAA CTCTTTGAAA AAACAGAATG TTATGATGAG 
CGGAAAAAAA TGGCAAGGGA TATTTATGAC AATTTTATAA TGGAGGAAAT GTTGTCCCAC 
ACCTATGAAT ACTCCAAACA TGCTGTTGCG AGTGTACAAA AATATTTATT AAAAAATGAG 
GTCCCGGTTG ACTTATTTGA ACCATACTTG GAAGAAATAT TTACACAACT TAAAGGAAAG 
CCATTTAAAA AATTTTTAGA AAGCGATAAA TTTACTAGGT TTTGCCAGTG GAAAAATTTA 
GAACTGAACA TACAACTAAC TATGAACGAT TTCAGCGTTC ATAGAATAAT TGGACGGGGG 
GGTTTCGGAG AAGTATATGG ATGCCGAAAA GCTGACACTG GAAAAATGTA TGCTATGAAA 
TGCTTGGACA AAAAACGGAT TAAAATGAAA CAAGGAGAAA TGCTAGCTCT TAATGAAAGA 
AATATGCTGC AAGCAGTCAG TACTGGAATT GATTGTCCGT TCATAGTGTG TATGACGTAT 
GCCTTTCATA CGCCGGATAA GCTATGTTTT ATATTAGATC TAATGAATGG TGGTGATCTA 
CATTACCATT TATCTCAACA TGGTATTTTC AGTGAGGATG AAATGAAATT TTATGCAGCG 
GAGGTGATTT TGGGATTGGA ACACATGCAT AAACGTTGCA TTGTGTACCG AGACTTAAAA 
CCCGCCAACA TACTGCTCGA TGAAAACGGA CACATACGAA TTTCTGATTT GGGATTGGCT 
TGCGACTTCT CAAAAAAGAA ACCGCATGCT TCAGTGGGAA CTCATGGATA CATGGCTCCA 
GAGGTGCTAT CTAAAGGAAC ATCATACGAT TCGTGCGCAG ATTGGTTTAG CTTCGGATGC 
ATGTTATATA AATTGCTTAA AGGTCATTCT CCATTTCGAC AGCATAAAAC AAAGGATAAG 
CTAGAAATTG ACAAAATGAC ATTAACTATG AACGTAGAAC TTCCAGAATC GTTTTCTTTG 
GAACTAAAGA ATTTGTTGGA AATGTTACTT CAAAGGGACG TCTCTAAAAG GCTTGGGTGC 
ATGGGAAATG GCGCGGATGA AGTAAAAATG CACAATTTTT TCTGTGGAAT TGATTGGCAC 
CAGGTTTACA TACAAAAATA TACTCCACCT TTAGTACCCC CTAGAGGGGA AGTAAATGCC 
GCAGATGCCT TCGATATTGG ATCATTTGAT GAGGAAGATA CCAAGGGCAT AAAACTAAAC 
GACGCCGATC AAGATCTATA TAAAATGTTT TCGTTGACAA TATCTGAAAG GTGGCAACAG 
GAAGTTTCTG AAACAGTATT TGATACAGTC AACACTGAGA CAGATAAACT AGAACAAAAA 
AGAAAATTGA AGCAAAAGCA GCACTTCGAT GCAGATGAAA AGGAATCCGA TTGTATATTA 
CATGGCTACA TAAAAAAGCT TGGGGGCTCT TTTGCATCTT TATGGCAAAC AAAATATGCC 
AAGCTATATC CAAATAGGCT GGAGCTACAT TCGGAAAGTG GAAATAATAA ACCAGAACTA 
ATTTTTATGG ACCAAGTAGA AGATATATCA TCGGATTTCA TTCTGCACAA AAACGAGAAT 
TGCATTCAAA TTCGCATTAA TGATGGAACC CGTGATGGCA GAATCATTTT GACAAACTCA 
GATGAAATAG GATTAAAAGA ATGGTCATCT TCCCTAAGAT CGGCACACAA AATTTCTCAA 
GACCTCTTGG GGTCAATGGC TAAAAAAGCT GGAAAAATAT ATGGCAGTGA AAGGGATGTC 
AACAAATCTA TGTATATTTT TGGTGGGAAC TGCTCAACGA AAACTTCTAA CGGGTCTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gprk1 ( NM_001169580.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163051.1 Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11822
Clone ID Related Accession (Same CDS sequence)	NM_001299206.1 , NM_001169580.2 , NM_001042973.3
Accession Version	NM_001169580.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	G protein-coupled receptor kinase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169580.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGATT TGGAAGCAGT CTTGGCTGAT GTCAGCTATT TAATGGCAAT GGAAAAGTCT 
AAATGTACTC CAGCTGCGAG GGCAAGCAAG AAATTAAATT TACCCGACCC GAGTGTTAGA 
AGTGTTATGT ATAAATATTT GGAAAAAGAA GGCGAATTGA ATTTTCATAA AATATTTAAC 
GAAGTTTTGG GCTATTTGTT GTTTAAAGAC TTTTGTGAAA ACGACTCAGA AGAACCTATA 
CAGCAACTTA AGTTTTTTGA ACAGATAAAA CTCTTTGAAA AAACAGAATG TTATGATGAG 
CGGAAAAAAA TGGCAAGGGA TATTTATGAC AATTTTATAA TGGAGGAAAT GTTGTCCCAC 
ACCTATGAAT ACTCCAAACA TGCTGTTGCG AGTGTACAAA AATATTTATT AAAAAATGAG 
GTCCCGGTTG ACTTATTTGA ACCATACTTG GAAGAAATAT TTACACAACT TAAAGGAAAG 
CCATTTAAAA AATTTTTAGA AAGCGATAAA TTTACTAGGT TTTGCCAGTG GAAAAATTTA 
GAACTGAACA TACAACTAAC TATGAACGAT TTCAGCGTTC ATAGAATAAT TGGACGGGGG 
GGTTTCGGAG AAGTATATGG ATGCCGAAAA GCTGACACTG GAAAAATGTA TGCTATGAAA 
TGCTTGGACA AAAAACGGAT TAAAATGAAA CAAGGAGAAA TGCTAGCTCT TAATGAAAGA 
AATATGCTGC AAGCAGTCAG TACTGGAATT GATTGTCCGT TCATAGTGTG TATGACGTAT 
GCCTTTCATA CGCCGGATAA GCTATGTTTT ATATTAGATC TAATGAATGG TGGTGATCTA 
CATTACCATT TATCTCAACA TGGTATTTTC AGTGAGGATG AAATGAAATT TTATGCAGCG 
GAGGTGATTT TGGGATTGGA ACACATGCAT AAACGTTGCA TTGTGTACCG AGACTTAAAA 
CCCGCCAACA TACTGCTCGA TGAAAACGGA CACATACGAA TTTCTGATTT GGGATTGGCT 
TGCGACTTCT CAAAAAAGAA ACCGCATGCT TCAGTGGGAA CTCATGGATA CATGGCTCCA 
GAGGTGCTAT CTAAAGGAAC ATCATACGAT TCGTGCGCAG ATTGGTTTAG CTTCGGATGC 
ATGTTATATA AATTGCTTAA AGGTCATTCT CCATTTCGAC AGCATAAAAC AAAGGATAAG 
CTAGAAATTG ACAAAATGAC ATTAACTATG AACGTAGAAC TTCCAGAATC GTTTTCTTTG 
GAACTAAAGA ATTTGTTGGA AATGTTACTT CAAAGGGACG TCTCTAAAAG GCTTGGGTGC 
ATGGGAAATG GCGCGGATGA AGTAAAAATG CACAATTTTT TCTGTGGAAT TGATTGGCAC 
CAGGTTTACA TACAAAAATA TACTCCACCT TTAGTACCCC CTAGAGGGGA AGTAAATGCC 
GCAGATGCCT TCGATATTGG ATCATTTGAT GAGGAAGATA CCAAGGGCAT AAAACTAAAC 
GACGCCGATC AAGATCTATA TAAAATGTTT TCGTTGACAA TATCTGAAAG GTGGCAACAG 
GAAGTTTCTG AAACAGTATT TGATACAGTC AACACTGAGA CAGATAAACT AGAACAAAAA 
AGAAAATTGA AGCAAAAGCA GCACTTCGAT GCAGATGAAA AGGAATCCGA TTGTATATTA 
CATGGCTACA TAAAAAAGCT TGGGGGCTCT TTTGCATCTT TATGGCAAAC AAAATATGCC 
AAGCTATATC CAAATAGGCT GGAGCTACAT TCGGAAAGTG GAAATAATAA ACCAGAACTA 
ATTTTTATGG ACCAAGTAGA AGATATATCA TCGGATTTCA TTCTGCACAA AAACGAGAAT 
TGCATTCAAA TTCGCATTAA TGATGGAACC CGTGATGGCA GAATCATTTT GACAAACTCA 
GATGAAATAG GATTAAAAGA ATGGTCATCT TCCCTAAGAT CGGCACACAA AATTTCTCAA 
GACCTCTTGG GGTCAATGGC TAAAAAAGCT GGAAAAATAT ATGGCAGTGA AAGGGATGTC 
AACAAATCTA TGTATATTTT TGGTGGGAAC TGCTCAACGA AAACTTCTAA CGGGTCTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163051.1
CDS	94..2196
Translation	MADLEAVLADVSYLMAMEKSKCTPAARASKKLNLPDPSVRSVMYKYLEKEGELNFHKIFNEVLGYLLFKDFCENDSEEPIQQLKFFEQIKLFEKTECYDERKKMARDIYDNFIMEEMLSHTYEYSKHAVASVQKYLLKNEVPVDLFEPYLEEIFTQLKGKPFKKFLESDKFTRFCQWKNLELNIQLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGEMLALNERNMLQAVSTGIDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGIFSEDEMKFYAAEVILGLEHMHKRCIVYRDLKPANILLDENGHIRISDLGLACDFSKKKPHASVGTHGYMAPEVLSKGTSYDSCADWFSFGCMLYKLLKGHSPFRQHKTKDKLEIDKMTLTMNVELPESFSLELKNLLEMLLQRDVSKRLGCMGNGADEVKMHNFFCGIDWHQVYIQKYTPPLVPPRGEVNAADAFDIGSFDEEDTKGIKLNDADQDLYKMFSLTISERWQQEVSETVFDTVNTETDKLEQKRKLKQKQHFDADEKESDCILHGYIKKLGGSFASLWQTKYAKLYPNRLELHSESGNNKPELIFMDQVEDISSDFILHKNENCIQIRINDGTRDGRIILTNSDEIGLKEWSSSLRSAHKISQDLLGSMAKKAGKIYGSERDVNKSMYIFGGNCSTKTSNGSN

Target ORF information:

RefSeq Version	NM_001169580.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169580.2

ORF Insert Sequence:

ATGGCTGATT TGGAAGCAGT CTTGGCTGAT GTCAGCTATT TAATGGCAAT GGAAAAGTCT 
AAATGTACTC CAGCTGCGAG GGCAAGCAAG AAATTAAATT TACCCGACCC GAGTGTTAGA 
AGTGTTATGT ATAAATATTT GGAAAAAGAA GGCGAATTGA ATTTTCATAA AATATTTAAC 
GAAGTTTTGG GCTATTTGTT GTTTAAAGAC TTTTGTGAAA ACGACTCAGA AGAACCTATA 
CAGCAACTTA AGTTTTTTGA ACAGATAAAA CTCTTTGAAA AAACAGAATG TTATGATGAG 
CGGAAAAAAA TGGCAAGGGA TATTTATGAC AATTTTATAA TGGAGGAAAT GTTGTCCCAC 
ACCTATGAAT ACTCCAAACA TGCTGTTGCG AGTGTACAAA AATATTTATT AAAAAATGAG 
GTCCCGGTTG ACTTATTTGA ACCATACTTG GAAGAAATAT TTACACAACT TAAAGGAAAG 
CCATTTAAAA AATTTTTAGA AAGCGATAAA TTTACTAGGT TTTGCCAGTG GAAAAATTTA 
GAACTGAACA TACAACTAAC TATGAACGAT TTCAGCGTTC ATAGAATAAT TGGACGGGGG 
GGTTTCGGAG AAGTATATGG ATGCCGAAAA GCTGACACTG GAAAAATGTA TGCTATGAAA 
TGCTTGGACA AAAAACGGAT TAAAATGAAA CAAGGAGAAA TGCTAGCTCT TAATGAAAGA 
AATATGCTGC AAGCAGTCAG TACTGGAATT GATTGTCCGT TCATAGTGTG TATGACGTAT 
GCCTTTCATA CGCCGGATAA GCTATGTTTT ATATTAGATC TAATGAATGG TGGTGATCTA 
CATTACCATT TATCTCAACA TGGTATTTTC AGTGAGGATG AAATGAAATT TTATGCAGCG 
GAGGTGATTT TGGGATTGGA ACACATGCAT AAACGTTGCA TTGTGTACCG AGACTTAAAA 
CCCGCCAACA TACTGCTCGA TGAAAACGGA CACATACGAA TTTCTGATTT GGGATTGGCT 
TGCGACTTCT CAAAAAAGAA ACCGCATGCT TCAGTGGGAA CTCATGGATA CATGGCTCCA 
GAGGTGCTAT CTAAAGGAAC ATCATACGAT TCGTGCGCAG ATTGGTTTAG CTTCGGATGC 
ATGTTATATA AATTGCTTAA AGGTCATTCT CCATTTCGAC AGCATAAAAC AAAGGATAAG 
CTAGAAATTG ACAAAATGAC ATTAACTATG AACGTAGAAC TTCCAGAATC GTTTTCTTTG 
GAACTAAAGA ATTTGTTGGA AATGTTACTT CAAAGGGACG TCTCTAAAAG GCTTGGGTGC 
ATGGGAAATG GCGCGGATGA AGTAAAAATG CACAATTTTT TCTGTGGAAT TGATTGGCAC 
CAGGTTTACA TACAAAAATA TACTCCACCT TTAGTACCCC CTAGAGGGGA AGTAAATGCC 
GCAGATGCCT TCGATATTGG ATCATTTGAT GAGGAAGATA CCAAGGGCAT AAAACTAAAC 
GACGCCGATC AAGATCTATA TAAAATGTTT TCGTTGACAA TATCTGAAAG GTGGCAACAG 
GAAGTTTCTG AAACAGTATT TGATACAGTC AACACTGAGA CAGATAAACT AGAACAAAAA 
AGAAAATTGA AGCAAAAGCA GCACTTCGAT GCAGATGAAA AGGAATCCGA TTGTATATTA 
CATGGCTACA TAAAAAAGCT TGGGGGCTCT TTTGCATCTT TATGGCAAAC AAAATATGCC 
AAGCTATATC CAAATAGGCT GGAGCTACAT TCGGAAAGTG GAAATAATAA ACCAGAACTA 
ATTTTTATGG ACCAAGTAGA AGATATATCA TCGGATTTCA TTCTGCACAA AAACGAGAAT 
TGCATTCAAA TTCGCATTAA TGATGGAACC CGTGATGGCA GAATCATTTT GACAAACTCA 
GATGAAATAG GATTAAAAGA ATGGTCATCT TCCCTAAGAT CGGCACACAA AATTTCTCAA 
GACCTCTTGG GGTCAATGGC TAAAAAAGCT GGAAAAATAT ATGGCAGTGA AAGGGATGTC 
AACAAATCTA TGTATATTTT TGGTGGGAAC TGCTCAACGA AAACTTCTAA CGGGTCTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gprk1 ( NM_001042973.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036438.1 Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11822
Clone ID Related Accession (Same CDS sequence)	NM_001299206.1 , NM_001169580.2 , NM_001042973.3
Accession Version	NM_001042973.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	G protein-coupled receptor kinase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001042973.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGATT TGGAAGCAGT CTTGGCTGAT GTCAGCTATT TAATGGCAAT GGAAAAGTCT 
AAATGTACTC CAGCTGCGAG GGCAAGCAAG AAATTAAATT TACCCGACCC GAGTGTTAGA 
AGTGTTATGT ATAAATATTT GGAAAAAGAA GGCGAATTGA ATTTTCATAA AATATTTAAC 
GAAGTTTTGG GCTATTTGTT GTTTAAAGAC TTTTGTGAAA ACGACTCAGA AGAACCTATA 
CAGCAACTTA AGTTTTTTGA ACAGATAAAA CTCTTTGAAA AAACAGAATG TTATGATGAG 
CGGAAAAAAA TGGCAAGGGA TATTTATGAC AATTTTATAA TGGAGGAAAT GTTGTCCCAC 
ACCTATGAAT ACTCCAAACA TGCTGTTGCG AGTGTACAAA AATATTTATT AAAAAATGAG 
GTCCCGGTTG ACTTATTTGA ACCATACTTG GAAGAAATAT TTACACAACT TAAAGGAAAG 
CCATTTAAAA AATTTTTAGA AAGCGATAAA TTTACTAGGT TTTGCCAGTG GAAAAATTTA 
GAACTGAACA TACAACTAAC TATGAACGAT TTCAGCGTTC ATAGAATAAT TGGACGGGGG 
GGTTTCGGAG AAGTATATGG ATGCCGAAAA GCTGACACTG GAAAAATGTA TGCTATGAAA 
TGCTTGGACA AAAAACGGAT TAAAATGAAA CAAGGAGAAA TGCTAGCTCT TAATGAAAGA 
AATATGCTGC AAGCAGTCAG TACTGGAATT GATTGTCCGT TCATAGTGTG TATGACGTAT 
GCCTTTCATA CGCCGGATAA GCTATGTTTT ATATTAGATC TAATGAATGG TGGTGATCTA 
CATTACCATT TATCTCAACA TGGTATTTTC AGTGAGGATG AAATGAAATT TTATGCAGCG 
GAGGTGATTT TGGGATTGGA ACACATGCAT AAACGTTGCA TTGTGTACCG AGACTTAAAA 
CCCGCCAACA TACTGCTCGA TGAAAACGGA CACATACGAA TTTCTGATTT GGGATTGGCT 
TGCGACTTCT CAAAAAAGAA ACCGCATGCT TCAGTGGGAA CTCATGGATA CATGGCTCCA 
GAGGTGCTAT CTAAAGGAAC ATCATACGAT TCGTGCGCAG ATTGGTTTAG CTTCGGATGC 
ATGTTATATA AATTGCTTAA AGGTCATTCT CCATTTCGAC AGCATAAAAC AAAGGATAAG 
CTAGAAATTG ACAAAATGAC ATTAACTATG AACGTAGAAC TTCCAGAATC GTTTTCTTTG 
GAACTAAAGA ATTTGTTGGA AATGTTACTT CAAAGGGACG TCTCTAAAAG GCTTGGGTGC 
ATGGGAAATG GCGCGGATGA AGTAAAAATG CACAATTTTT TCTGTGGAAT TGATTGGCAC 
CAGGTTTACA TACAAAAATA TACTCCACCT TTAGTACCCC CTAGAGGGGA AGTAAATGCC 
GCAGATGCCT TCGATATTGG ATCATTTGAT GAGGAAGATA CCAAGGGCAT AAAACTAAAC 
GACGCCGATC AAGATCTATA TAAAATGTTT TCGTTGACAA TATCTGAAAG GTGGCAACAG 
GAAGTTTCTG AAACAGTATT TGATACAGTC AACACTGAGA CAGATAAACT AGAACAAAAA 
AGAAAATTGA AGCAAAAGCA GCACTTCGAT GCAGATGAAA AGGAATCCGA TTGTATATTA 
CATGGCTACA TAAAAAAGCT TGGGGGCTCT TTTGCATCTT TATGGCAAAC AAAATATGCC 
AAGCTATATC CAAATAGGCT GGAGCTACAT TCGGAAAGTG GAAATAATAA ACCAGAACTA 
ATTTTTATGG ACCAAGTAGA AGATATATCA TCGGATTTCA TTCTGCACAA AAACGAGAAT 
TGCATTCAAA TTCGCATTAA TGATGGAACC CGTGATGGCA GAATCATTTT GACAAACTCA 
GATGAAATAG GATTAAAAGA ATGGTCATCT TCCCTAAGAT CGGCACACAA AATTTCTCAA 
GACCTCTTGG GGTCAATGGC TAAAAAAGCT GGAAAAATAT ATGGCAGTGA AAGGGATGTC 
AACAAATCTA TGTATATTTT TGGTGGGAAC TGCTCAACGA AAACTTCTAA CGGGTCTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036438.1
CDS	179..2281
Translation	MADLEAVLADVSYLMAMEKSKCTPAARASKKLNLPDPSVRSVMYKYLEKEGELNFHKIFNEVLGYLLFKDFCENDSEEPIQQLKFFEQIKLFEKTECYDERKKMARDIYDNFIMEEMLSHTYEYSKHAVASVQKYLLKNEVPVDLFEPYLEEIFTQLKGKPFKKFLESDKFTRFCQWKNLELNIQLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGEMLALNERNMLQAVSTGIDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGIFSEDEMKFYAAEVILGLEHMHKRCIVYRDLKPANILLDENGHIRISDLGLACDFSKKKPHASVGTHGYMAPEVLSKGTSYDSCADWFSFGCMLYKLLKGHSPFRQHKTKDKLEIDKMTLTMNVELPESFSLELKNLLEMLLQRDVSKRLGCMGNGADEVKMHNFFCGIDWHQVYIQKYTPPLVPPRGEVNAADAFDIGSFDEEDTKGIKLNDADQDLYKMFSLTISERWQQEVSETVFDTVNTETDKLEQKRKLKQKQHFDADEKESDCILHGYIKKLGGSFASLWQTKYAKLYPNRLELHSESGNNKPELIFMDQVEDISSDFILHKNENCIQIRINDGTRDGRIILTNSDEIGLKEWSSSLRSAHKISQDLLGSMAKKAGKIYGSERDVNKSMYIFGGNCSTKTSNGSN

Target ORF information:

RefSeq Version	NM_001042973.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein-coupled receptor kinase 1 (Gprk1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042973.3

ORF Insert Sequence:

ATGGCTGATT TGGAAGCAGT CTTGGCTGAT GTCAGCTATT TAATGGCAAT GGAAAAGTCT 
AAATGTACTC CAGCTGCGAG GGCAAGCAAG AAATTAAATT TACCCGACCC GAGTGTTAGA 
AGTGTTATGT ATAAATATTT GGAAAAAGAA GGCGAATTGA ATTTTCATAA AATATTTAAC 
GAAGTTTTGG GCTATTTGTT GTTTAAAGAC TTTTGTGAAA ACGACTCAGA AGAACCTATA 
CAGCAACTTA AGTTTTTTGA ACAGATAAAA CTCTTTGAAA AAACAGAATG TTATGATGAG 
CGGAAAAAAA TGGCAAGGGA TATTTATGAC AATTTTATAA TGGAGGAAAT GTTGTCCCAC 
ACCTATGAAT ACTCCAAACA TGCTGTTGCG AGTGTACAAA AATATTTATT AAAAAATGAG 
GTCCCGGTTG ACTTATTTGA ACCATACTTG GAAGAAATAT TTACACAACT TAAAGGAAAG 
CCATTTAAAA AATTTTTAGA AAGCGATAAA TTTACTAGGT TTTGCCAGTG GAAAAATTTA 
GAACTGAACA TACAACTAAC TATGAACGAT TTCAGCGTTC ATAGAATAAT TGGACGGGGG 
GGTTTCGGAG AAGTATATGG ATGCCGAAAA GCTGACACTG GAAAAATGTA TGCTATGAAA 
TGCTTGGACA AAAAACGGAT TAAAATGAAA CAAGGAGAAA TGCTAGCTCT TAATGAAAGA 
AATATGCTGC AAGCAGTCAG TACTGGAATT GATTGTCCGT TCATAGTGTG TATGACGTAT 
GCCTTTCATA CGCCGGATAA GCTATGTTTT ATATTAGATC TAATGAATGG TGGTGATCTA 
CATTACCATT TATCTCAACA TGGTATTTTC AGTGAGGATG AAATGAAATT TTATGCAGCG 
GAGGTGATTT TGGGATTGGA ACACATGCAT AAACGTTGCA TTGTGTACCG AGACTTAAAA 
CCCGCCAACA TACTGCTCGA TGAAAACGGA CACATACGAA TTTCTGATTT GGGATTGGCT 
TGCGACTTCT CAAAAAAGAA ACCGCATGCT TCAGTGGGAA CTCATGGATA CATGGCTCCA 
GAGGTGCTAT CTAAAGGAAC ATCATACGAT TCGTGCGCAG ATTGGTTTAG CTTCGGATGC 
ATGTTATATA AATTGCTTAA AGGTCATTCT CCATTTCGAC AGCATAAAAC AAAGGATAAG 
CTAGAAATTG ACAAAATGAC ATTAACTATG AACGTAGAAC TTCCAGAATC GTTTTCTTTG 
GAACTAAAGA ATTTGTTGGA AATGTTACTT CAAAGGGACG TCTCTAAAAG GCTTGGGTGC 
ATGGGAAATG GCGCGGATGA AGTAAAAATG CACAATTTTT TCTGTGGAAT TGATTGGCAC 
CAGGTTTACA TACAAAAATA TACTCCACCT TTAGTACCCC CTAGAGGGGA AGTAAATGCC 
GCAGATGCCT TCGATATTGG ATCATTTGAT GAGGAAGATA CCAAGGGCAT AAAACTAAAC 
GACGCCGATC AAGATCTATA TAAAATGTTT TCGTTGACAA TATCTGAAAG GTGGCAACAG 
GAAGTTTCTG AAACAGTATT TGATACAGTC AACACTGAGA CAGATAAACT AGAACAAAAA 
AGAAAATTGA AGCAAAAGCA GCACTTCGAT GCAGATGAAA AGGAATCCGA TTGTATATTA 
CATGGCTACA TAAAAAAGCT TGGGGGCTCT TTTGCATCTT TATGGCAAAC AAAATATGCC 
AAGCTATATC CAAATAGGCT GGAGCTACAT TCGGAAAGTG GAAATAATAA ACCAGAACTA 
ATTTTTATGG ACCAAGTAGA AGATATATCA TCGGATTTCA TTCTGCACAA AAACGAGAAT 
TGCATTCAAA TTCGCATTAA TGATGGAACC CGTGATGGCA GAATCATTTT GACAAACTCA 
GATGAAATAG GATTAAAAGA ATGGTCATCT TCCCTAAGAT CGGCACACAA AATTTCTCAA 
GACCTCTTGG GGTCAATGGC TAAAAAAGCT GGAAAAATAT ATGGCAGTGA AAGGGATGTC 
AACAAATCTA TGTATATTTT TGGTGGGAAC TGCTCAACGA AAACTTCTAA CGGGTCTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gprk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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