RhoGAPp190 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGAPp190
Entrez Gene ID	32743
Full Name	Rho GTPase activating protein p190
Synonyms	8267,BcDNA:GH17919,BcDNA:GM05133,CG32555,CG8240,CG8267,D-p190,DRhoGAP,Dmel\CG32555,RhoGAP,RhoGAP P190,RhoGAP-16B12,mdcds_56566,p190,p190 RhoGAP,p190-RhoGAP,p190RhoGAP
General protein information	Preferred Names Rho GTPase activating protein p190 Names CG32555 gene product from transcript CG32555-RG CG32555-PA CG32555-PB CG32555-PC CG32555-PD CG32555-PE CG32555-PF CG32555-PG RhoGAPp190-PA RhoGAPp190-PB RhoGAPp190-PC RhoGAPp190-PD RhoGAPp190-PE RhoGAPp190-PF RhoGAPp190-PG Rp190RhoGAP
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 16B10-16B10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272728.1	NP_001259657.1	Rho GTPase activating protein p190, isoform E
NM_001272729.1	NP_001259658.1	Rho GTPase activating protein p190, isoform F
NM_001272730.1	NP_001259659.1	Rho GTPase activating protein p190, isoform G
NM_133003.3	NP_573231.2	Rho GTPase activating protein p190, isoform B
NM_001272727.1	NP_001259656.1	Rho GTPase activating protein p190, isoform D
NM_167572.2	NP_728089.1	Rho GTPase activating protein p190, isoform A
NM_167573.2	NP_728090.1	Rho GTPase activating protein p190, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGAP35	XP_003316510.1
Macaca mulatta (Rhesus monkey)	GRLF1	XP_001112719.1
Rattus norvegicus (Norway rat)	Arhgap35	NP_001258061.1
Gallus gallus (chicken)	ARHGAP35	XP_003643808.2
Homo sapiens (human)	ARHGAP35	NP_004482.4
Bos taurus (cattle)	ARHGAP35	NP_001179327.1
Danio rerio (zebrafish)	arhgap35	XP_003198691.2
Danio rerio (zebrafish)	LOC100004299	XP_001343636.1
Xenopus tropicalis (tropical clawed frog)	arhgap35	XP_004917009.1
Drosophila melanogaster (fruit fly)	RhoGAPp190	NP_001259658.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000870	XP_316844.5
Mus musculus (house mouse)	Grlf1	NP_766327.3
Canis lupus familiaris (dog)	ARHGAP35	NP_001003022.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RhoGAPp190 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGAPp190 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03700	NM_001272730.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFa01923	NM_167572.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFa01923	NM_133003.3 Latest version!	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFa01923	NM_167573.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFa03701	NM_001272729.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFa03702	NM_001272728.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFa03703	NM_001272727.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGAPp190 ( NM_001272730.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259659.1 Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa03700
Clone ID Related Accession (Same CDS sequence)	NM_001272730.1
Accession Version	NM_001272730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein p190, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAACT GCACCGAATC GGACAGCTGT 
GCCAGTTCCA CGGAGCGACG GGTGCGCCAG CAGAATGCCT ACTATAAGGC CAGCAAGAAG 
CCGGTGGCCG CCAAGAAGCA GAAAAAGAAG AAGGTGGCAA TTCCGGTGCA GACACCGCGT 
GTTCCTCCGT TCGGCTCGTA TGTGAGTCCG CCGGAGATTC CGCTGCACTA CCAGCGCATG 
GCCGTTGGCG GCAGCGGGCC AGGTGAGAAA AAGAAACCAG AGCCATGTGT TCCCGAGTTC 
ATGAAGAGTG ACAAGTCGCC AGAGTATTCC ATGGTGCCAG AACTGGCGGG TGCCGGCATC 
TTCGGTGCAG AGAATCTGCC CGAGTACAAC ATGAACCAGG CCAAATGCTT GAAGGACTTT 
GAGAAGCTGG AGAAGCGGCG CATCAAAGAG GAAACGGCCA GGCAGCGCAA ACTACAGGAG 
AAGGAGAAGG AGCAAGAGAA GAAGCTCAAG CGCAAACTAA AGCAGAATGC CAAGGGCCTG 
GTTGAATCCG CGGAGGCGCA ATTCGGCAAG CTGATGATTA CCTCCGAGCA AGGCGAGATC 
CCCATCTTCC TCAACAAGTG CGTCGAGTTT ATCGAGAAGG AGGGCCTCGA CTCCGAGGGC 
ATTTATAGGG TGCCCGGAAG TAGGGCGCAT GTCGACATGT TGTTCCAACG CTTCGAAGAG 
GATACCAATA CTGAGATTGA TGCGCTCGAC ATTCCCGTCA ATGCCGTGGC CACGGCACTG 
AAGGACTTCT TCTCCAAGCG CCTGCCTCCG CTGTTCAGCA AGGACATCAT CAAGGAGCTG 
GAGGAGATTG CCGGCTCGCG AGGCGTCGGC AATTCCAAAC TGAATGTGGA GGTCAAAACG 
GACCGAAGTT GCCGCTTGAT AGCTTTAAAA TCGCTGCTCC AGAAGCTGCC GCCCATCAAC 
TTTGCCATAC TCAAATACAT ATTCCAACAC TTTGTGCACG TATCAGACAA TTCGAAGCTG 
AATAGCATGG ACAGCAAGAA CTTGGCCATT TGCTGGTGGC CGACACTCAT ACCCATCGAC 
TTCACCGACA TGGGCCACTT CGAGCAGCTG CGTCCGTATC TGGAGGACAT TGTCCAGACA 
ATGATTGACC AGTTCCCGTA TCTGTTCTGC GGCAAGGATG CTTTTGTCAT GGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259659.1
CDS	75..4271
Translation	MRQFNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGDVRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEKEYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYELYVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELLDTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNKKLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEAEDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMGRECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQEDSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWKSSGSGSDRTLNLLIVGSEHLASDLLNDIRICTGSKGEYIYENQTYYLNYRIANGDMEAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDKFENLPIVLVYQPQDLKENEVEYLRNEGMRLSEMLHCDFIDHTQNHQKYVYDILNIVILSLKLTEMKSYEPYPSNHTDLRILCCIFCGDQYDIENIVQPLVEESTLVKANEHSIIVDVFIGDAKRRVEFILSSYHGTSQYRDELIHGYIYFYSTKRRSSLANLSILAAQNANIPLQIIAVTESGGVNAFFNSDICQFLITEGNAVADRFKGSFMTFSADQYVKFAFYNPFLKTAWDNKYEVENLHVEESITLDSGEGTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEERDISLDEIYDDNEKPKHLHQNCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGSGPGEKKKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIPIFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLFCGKDAFVMV

Target ORF information:

RefSeq Version	NM_001272730.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272730.1

ORF Insert Sequence:

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAACT GCACCGAATC GGACAGCTGT 
GCCAGTTCCA CGGAGCGACG GGTGCGCCAG CAGAATGCCT ACTATAAGGC CAGCAAGAAG 
CCGGTGGCCG CCAAGAAGCA GAAAAAGAAG AAGGTGGCAA TTCCGGTGCA GACACCGCGT 
GTTCCTCCGT TCGGCTCGTA TGTGAGTCCG CCGGAGATTC CGCTGCACTA CCAGCGCATG 
GCCGTTGGCG GCAGCGGGCC AGGTGAGAAA AAGAAACCAG AGCCATGTGT TCCCGAGTTC 
ATGAAGAGTG ACAAGTCGCC AGAGTATTCC ATGGTGCCAG AACTGGCGGG TGCCGGCATC 
TTCGGTGCAG AGAATCTGCC CGAGTACAAC ATGAACCAGG CCAAATGCTT GAAGGACTTT 
GAGAAGCTGG AGAAGCGGCG CATCAAAGAG GAAACGGCCA GGCAGCGCAA ACTACAGGAG 
AAGGAGAAGG AGCAAGAGAA GAAGCTCAAG CGCAAACTAA AGCAGAATGC CAAGGGCCTG 
GTTGAATCCG CGGAGGCGCA ATTCGGCAAG CTGATGATTA CCTCCGAGCA AGGCGAGATC 
CCCATCTTCC TCAACAAGTG CGTCGAGTTT ATCGAGAAGG AGGGCCTCGA CTCCGAGGGC 
ATTTATAGGG TGCCCGGAAG TAGGGCGCAT GTCGACATGT TGTTCCAACG CTTCGAAGAG 
GATACCAATA CTGAGATTGA TGCGCTCGAC ATTCCCGTCA ATGCCGTGGC CACGGCACTG 
AAGGACTTCT TCTCCAAGCG CCTGCCTCCG CTGTTCAGCA AGGACATCAT CAAGGAGCTG 
GAGGAGATTG CCGGCTCGCG AGGCGTCGGC AATTCCAAAC TGAATGTGGA GGTCAAAACG 
GACCGAAGTT GCCGCTTGAT AGCTTTAAAA TCGCTGCTCC AGAAGCTGCC GCCCATCAAC 
TTTGCCATAC TCAAATACAT ATTCCAACAC TTTGTGCACG TATCAGACAA TTCGAAGCTG 
AATAGCATGG ACAGCAAGAA CTTGGCCATT TGCTGGTGGC CGACACTCAT ACCCATCGAC 
TTCACCGACA TGGGCCACTT CGAGCAGCTG CGTCCGTATC TGGAGGACAT TGTCCAGACA 
ATGATTGACC AGTTCCCGTA TCTGTTCTGC GGCAAGGATG CTTTTGTCAT GGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAPp190 ( NM_133003.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573231.2 Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01923
Clone ID Related Accession (Same CDS sequence)	NM_133003.3 , NM_167572.2 , NM_167573.2
Accession Version	NM_133003.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein p190, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_133003.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAAGT GGCTGGAGGA CAAGAGCGAT 
GGGCGACGCA ACATGAACAA GAATTTGATT TGGAACAACT TCAGCGGATC CACACATGCC 
TACACCACCG GTCGCCGTCA CATCGACTCC AATTTGAACA AAATCCGCCC GAAGGGACCC 
AGTCAAACGC TCAAAGTGGG CGAGGCGCCC AGTCGCAATT GCCCGGCCAT GAGCTCCTCC 
ACCTTCACCC TGCCCACGCA GCAGCCGGGC AAGCTGAACA TGAAGAACTT CCAGCTGGTC 
AGCGATGCGG TGGCCAAGAT GAATTTCACC GGCTCCGGTT CGGGTTCGGG TTCCGGCTCG 
GGATCGGGCA GCACTGGACT GGGCCTGGGT CTGGGCTCCG GCAGTGGCTG CATGGGTGAT 
TCGTTTTTGG AGCCGGTCGA CAAGGACGGC AAGCGCTACG ATCATGCCCA ATTGGATGGC 
GAGGATGAGG ATTCCGAGGA GCTCGCCGAG TACGAGCAGA TCTACGAAAA TGAAGACTGC 
ACCGAATCGG ACAGCTGTGC CAGTTCCACG GAGCGACGGG TGCGCCAGCA GAATGCCTAC 
TATAAGGCCA GCAAGAAGCC GGTGGCCGCC AAGAAGCAGA AAAAGAAGAA GGTGGCAATT 
CCGGTGCAGA CACCGCGTGT TCCTCCGTTC GGCTCGTATG TGAGTCCGCC GGAGATTCCG 
CTGCACTACC AGCGCATGGC CGTTGGCGGC AGCGGGCCAG AGAAACCAGA GCCATGTGTT 
CCCGAGTTCA TGAAGAGTGA CAAGTCGCCA GAGTATTCCA TGGTGCCAGA ACTGGCGGGT 
GCCGGCATCT TCGGTGCAGA GAATCTGCCC GAGTACAACA TGAACCAGGC CAAATGCTTG 
AAGGACTTTG AGAAGCTGGA GAAGCGGCGC ATCAAAGAGG AAACGGCCAG GCAGCGCAAA 
CTACAGGAGA AGGAGAAGGA GCAAGAGAAG AAGCTCAAGC GCAAACTAAA GCAGAATGCC 
AAGGGCCTGG TTGAATCCGC GGAGGCGCAA TTCGGCAAGC TGATGATTAC CTCCGAGCAA 
GGCGAGATCC CCATCTTCCT CAACAAGTGC GTCGAGTTTA TCGAGAAGGA GGGCCTCGAC 
TCCGAGGGCA TTTATAGGGT GCCCGGAAGT AGGGCGCATG TCGACATGTT GTTCCAACGC 
TTCGAAGAGG ATACCAATAC TGAGATTGAT GCGCTCGACA TTCCCGTCAA TGCCGTGGCC 
ACGGCACTGA AGGACTTCTT CTCCAAGCGC CTGCCTCCGC TGTTCAGCAA GGACATCATC 
AAGGAGCTGG AGGAGATTGC CGGCTCGCGA GGCGTCGGCA ATTCCAAACT GAATGTGGAG 
GTCAAAACGG ACCGAAGTTG CCGCTTGATA GCTTTAAAAT CGCTGCTCCA GAAGCTGCCG 
CCCATCAACT TTGCCATACT CAAATACATA TTCCAACACT TTGTGCACGT ATCAGACAAT 
TCGAAGCTGA ATAGCATGGA CAGCAAGAAC TTGGCCATTT GCTGGTGGCC GACACTCATA 
CCCATCGACT TCACCGACAT GGGCCACTTC GAGCAGCTGC GTCCGTATCT GGAGGACATT 
GTCCAGACAA TGATTGACCA GTTCCCGTAT CTGTTCTGCG GCAAGGATGC TTTTGTCATG 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573231.2
CDS	75..4760
Translation	MRQFNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGDVRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEKEYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYELYVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELLDTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNKKLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEAEDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMGRECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQEDSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWKSSGSGSDRTLNLLIVGSEHLASDLLNDIRICTGSKGEYIYENQTYYLNYRIANGDMEAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDKFENLPIVLVYQPQDLKENEVEYLRNEGMRLSEMLHCDFIDHTQNHQKYVYDILNIVILSLKLTEMKSYEPYPSNHTDLRILCCIFCGDQYDIENIVQPLVEESTLVKANEHSIIVDVFIGDAKRRVEFILSSYHGTSQYRDELIHGYIYFYSTKRRSSLANLSILAAQNANIPLQIIAVTESGGVNAFFNSDICQFLITEGNAVADRFKGSFMTFSADQYVKFAFYNPFLKTAWDNKYEVENLHVEESITLDSGEGTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEERDISLDEIYDDNEKPKHLHQKWLEDKSDGRRNMNKNLIWNNFSGSTHAYTTGRRHIDSNLNKIRPKGPSQTLKVGEAPSRNCPAMSSSTFTLPTQQPGKLNMKNFQLVSDAVAKMNFTGSGSGSGSGSGSGSTGLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQIYENEDCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGSGPEKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIPIFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLFCGKDAFVMV

Target ORF information:

RefSeq Version	NM_133003.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133003.3

ORF Insert Sequence:

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAAGT GGCTGGAGGA CAAGAGCGAT 
GGGCGACGCA ACATGAACAA GAATTTGATT TGGAACAACT TCAGCGGATC CACACATGCC 
TACACCACCG GTCGCCGTCA CATCGACTCC AATTTGAACA AAATCCGCCC GAAGGGACCC 
AGTCAAACGC TCAAAGTGGG CGAGGCGCCC AGTCGCAATT GCCCGGCCAT GAGCTCCTCC 
ACCTTCACCC TGCCCACGCA GCAGCCGGGC AAGCTGAACA TGAAGAACTT CCAGCTGGTC 
AGCGATGCGG TGGCCAAGAT GAATTTCACC GGCTCCGGTT CGGGTTCGGG TTCCGGCTCG 
GGATCGGGCA GCACTGGACT GGGCCTGGGT CTGGGCTCCG GCAGTGGCTG CATGGGTGAT 
TCGTTTTTGG AGCCGGTCGA CAAGGACGGC AAGCGCTACG ATCATGCCCA ATTGGATGGC 
GAGGATGAGG ATTCCGAGGA GCTCGCCGAG TACGAGCAGA TCTACGAAAA TGAAGACTGC 
ACCGAATCGG ACAGCTGTGC CAGTTCCACG GAGCGACGGG TGCGCCAGCA GAATGCCTAC 
TATAAGGCCA GCAAGAAGCC GGTGGCCGCC AAGAAGCAGA AAAAGAAGAA GGTGGCAATT 
CCGGTGCAGA CACCGCGTGT TCCTCCGTTC GGCTCGTATG TGAGTCCGCC GGAGATTCCG 
CTGCACTACC AGCGCATGGC CGTTGGCGGC AGCGGGCCAG AGAAACCAGA GCCATGTGTT 
CCCGAGTTCA TGAAGAGTGA CAAGTCGCCA GAGTATTCCA TGGTGCCAGA ACTGGCGGGT 
GCCGGCATCT TCGGTGCAGA GAATCTGCCC GAGTACAACA TGAACCAGGC CAAATGCTTG 
AAGGACTTTG AGAAGCTGGA GAAGCGGCGC ATCAAAGAGG AAACGGCCAG GCAGCGCAAA 
CTACAGGAGA AGGAGAAGGA GCAAGAGAAG AAGCTCAAGC GCAAACTAAA GCAGAATGCC 
AAGGGCCTGG TTGAATCCGC GGAGGCGCAA TTCGGCAAGC TGATGATTAC CTCCGAGCAA 
GGCGAGATCC CCATCTTCCT CAACAAGTGC GTCGAGTTTA TCGAGAAGGA GGGCCTCGAC 
TCCGAGGGCA TTTATAGGGT GCCCGGAAGT AGGGCGCATG TCGACATGTT GTTCCAACGC 
TTCGAAGAGG ATACCAATAC TGAGATTGAT GCGCTCGACA TTCCCGTCAA TGCCGTGGCC 
ACGGCACTGA AGGACTTCTT CTCCAAGCGC CTGCCTCCGC TGTTCAGCAA GGACATCATC 
AAGGAGCTGG AGGAGATTGC CGGCTCGCGA GGCGTCGGCA ATTCCAAACT GAATGTGGAG 
GTCAAAACGG ACCGAAGTTG CCGCTTGATA GCTTTAAAAT CGCTGCTCCA GAAGCTGCCG 
CCCATCAACT TTGCCATACT CAAATACATA TTCCAACACT TTGTGCACGT ATCAGACAAT 
TCGAAGCTGA ATAGCATGGA CAGCAAGAAC TTGGCCATTT GCTGGTGGCC GACACTCATA 
CCCATCGACT TCACCGACAT GGGCCACTTC GAGCAGCTGC GTCCGTATCT GGAGGACATT 
GTCCAGACAA TGATTGACCA GTTCCCGTAT CTGTTCTGCG GCAAGGATGC TTTTGTCATG 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAPp190 ( NM_167572.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728089.1 Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant A, mRNA.

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CloneID	OFa01923
Clone ID Related Accession (Same CDS sequence)	NM_133003.3 , NM_167572.2 , NM_167573.2
Accession Version	NM_167572.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein p190, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167572.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAAGT GGCTGGAGGA CAAGAGCGAT 
GGGCGACGCA ACATGAACAA GAATTTGATT TGGAACAACT TCAGCGGATC CACACATGCC 
TACACCACCG GTCGCCGTCA CATCGACTCC AATTTGAACA AAATCCGCCC GAAGGGACCC 
AGTCAAACGC TCAAAGTGGG CGAGGCGCCC AGTCGCAATT GCCCGGCCAT GAGCTCCTCC 
ACCTTCACCC TGCCCACGCA GCAGCCGGGC AAGCTGAACA TGAAGAACTT CCAGCTGGTC 
AGCGATGCGG TGGCCAAGAT GAATTTCACC GGCTCCGGTT CGGGTTCGGG TTCCGGCTCG 
GGATCGGGCA GCACTGGACT GGGCCTGGGT CTGGGCTCCG GCAGTGGCTG CATGGGTGAT 
TCGTTTTTGG AGCCGGTCGA CAAGGACGGC AAGCGCTACG ATCATGCCCA ATTGGATGGC 
GAGGATGAGG ATTCCGAGGA GCTCGCCGAG TACGAGCAGA TCTACGAAAA TGAAGACTGC 
ACCGAATCGG ACAGCTGTGC CAGTTCCACG GAGCGACGGG TGCGCCAGCA GAATGCCTAC 
TATAAGGCCA GCAAGAAGCC GGTGGCCGCC AAGAAGCAGA AAAAGAAGAA GGTGGCAATT 
CCGGTGCAGA CACCGCGTGT TCCTCCGTTC GGCTCGTATG TGAGTCCGCC GGAGATTCCG 
CTGCACTACC AGCGCATGGC CGTTGGCGGC AGCGGGCCAG AGAAACCAGA GCCATGTGTT 
CCCGAGTTCA TGAAGAGTGA CAAGTCGCCA GAGTATTCCA TGGTGCCAGA ACTGGCGGGT 
GCCGGCATCT TCGGTGCAGA GAATCTGCCC GAGTACAACA TGAACCAGGC CAAATGCTTG 
AAGGACTTTG AGAAGCTGGA GAAGCGGCGC ATCAAAGAGG AAACGGCCAG GCAGCGCAAA 
CTACAGGAGA AGGAGAAGGA GCAAGAGAAG AAGCTCAAGC GCAAACTAAA GCAGAATGCC 
AAGGGCCTGG TTGAATCCGC GGAGGCGCAA TTCGGCAAGC TGATGATTAC CTCCGAGCAA 
GGCGAGATCC CCATCTTCCT CAACAAGTGC GTCGAGTTTA TCGAGAAGGA GGGCCTCGAC 
TCCGAGGGCA TTTATAGGGT GCCCGGAAGT AGGGCGCATG TCGACATGTT GTTCCAACGC 
TTCGAAGAGG ATACCAATAC TGAGATTGAT GCGCTCGACA TTCCCGTCAA TGCCGTGGCC 
ACGGCACTGA AGGACTTCTT CTCCAAGCGC CTGCCTCCGC TGTTCAGCAA GGACATCATC 
AAGGAGCTGG AGGAGATTGC CGGCTCGCGA GGCGTCGGCA ATTCCAAACT GAATGTGGAG 
GTCAAAACGG ACCGAAGTTG CCGCTTGATA GCTTTAAAAT CGCTGCTCCA GAAGCTGCCG 
CCCATCAACT TTGCCATACT CAAATACATA TTCCAACACT TTGTGCACGT ATCAGACAAT 
TCGAAGCTGA ATAGCATGGA CAGCAAGAAC TTGGCCATTT GCTGGTGGCC GACACTCATA 
CCCATCGACT TCACCGACAT GGGCCACTTC GAGCAGCTGC GTCCGTATCT GGAGGACATT 
GTCCAGACAA TGATTGACCA GTTCCCGTAT CTGTTCTGCG GCAAGGATGC TTTTGTCATG 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728089.1
CDS	192..4877
Translation	MRQFNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGDVRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEKEYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYELYVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELLDTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNKKLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEAEDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMGRECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQEDSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWKSSGSGSDRTLNLLIVGSEHLASDLLNDIRICTGSKGEYIYENQTYYLNYRIANGDMEAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDKFENLPIVLVYQPQDLKENEVEYLRNEGMRLSEMLHCDFIDHTQNHQKYVYDILNIVILSLKLTEMKSYEPYPSNHTDLRILCCIFCGDQYDIENIVQPLVEESTLVKANEHSIIVDVFIGDAKRRVEFILSSYHGTSQYRDELIHGYIYFYSTKRRSSLANLSILAAQNANIPLQIIAVTESGGVNAFFNSDICQFLITEGNAVADRFKGSFMTFSADQYVKFAFYNPFLKTAWDNKYEVENLHVEESITLDSGEGTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEERDISLDEIYDDNEKPKHLHQKWLEDKSDGRRNMNKNLIWNNFSGSTHAYTTGRRHIDSNLNKIRPKGPSQTLKVGEAPSRNCPAMSSSTFTLPTQQPGKLNMKNFQLVSDAVAKMNFTGSGSGSGSGSGSGSTGLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQIYENEDCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGSGPEKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIPIFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLFCGKDAFVMV

Target ORF information:

RefSeq Version	NM_167572.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167572.2

ORF Insert Sequence:

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAAGT GGCTGGAGGA CAAGAGCGAT 
GGGCGACGCA ACATGAACAA GAATTTGATT TGGAACAACT TCAGCGGATC CACACATGCC 
TACACCACCG GTCGCCGTCA CATCGACTCC AATTTGAACA AAATCCGCCC GAAGGGACCC 
AGTCAAACGC TCAAAGTGGG CGAGGCGCCC AGTCGCAATT GCCCGGCCAT GAGCTCCTCC 
ACCTTCACCC TGCCCACGCA GCAGCCGGGC AAGCTGAACA TGAAGAACTT CCAGCTGGTC 
AGCGATGCGG TGGCCAAGAT GAATTTCACC GGCTCCGGTT CGGGTTCGGG TTCCGGCTCG 
GGATCGGGCA GCACTGGACT GGGCCTGGGT CTGGGCTCCG GCAGTGGCTG CATGGGTGAT 
TCGTTTTTGG AGCCGGTCGA CAAGGACGGC AAGCGCTACG ATCATGCCCA ATTGGATGGC 
GAGGATGAGG ATTCCGAGGA GCTCGCCGAG TACGAGCAGA TCTACGAAAA TGAAGACTGC 
ACCGAATCGG ACAGCTGTGC CAGTTCCACG GAGCGACGGG TGCGCCAGCA GAATGCCTAC 
TATAAGGCCA GCAAGAAGCC GGTGGCCGCC AAGAAGCAGA AAAAGAAGAA GGTGGCAATT 
CCGGTGCAGA CACCGCGTGT TCCTCCGTTC GGCTCGTATG TGAGTCCGCC GGAGATTCCG 
CTGCACTACC AGCGCATGGC CGTTGGCGGC AGCGGGCCAG AGAAACCAGA GCCATGTGTT 
CCCGAGTTCA TGAAGAGTGA CAAGTCGCCA GAGTATTCCA TGGTGCCAGA ACTGGCGGGT 
GCCGGCATCT TCGGTGCAGA GAATCTGCCC GAGTACAACA TGAACCAGGC CAAATGCTTG 
AAGGACTTTG AGAAGCTGGA GAAGCGGCGC ATCAAAGAGG AAACGGCCAG GCAGCGCAAA 
CTACAGGAGA AGGAGAAGGA GCAAGAGAAG AAGCTCAAGC GCAAACTAAA GCAGAATGCC 
AAGGGCCTGG TTGAATCCGC GGAGGCGCAA TTCGGCAAGC TGATGATTAC CTCCGAGCAA 
GGCGAGATCC CCATCTTCCT CAACAAGTGC GTCGAGTTTA TCGAGAAGGA GGGCCTCGAC 
TCCGAGGGCA TTTATAGGGT GCCCGGAAGT AGGGCGCATG TCGACATGTT GTTCCAACGC 
TTCGAAGAGG ATACCAATAC TGAGATTGAT GCGCTCGACA TTCCCGTCAA TGCCGTGGCC 
ACGGCACTGA AGGACTTCTT CTCCAAGCGC CTGCCTCCGC TGTTCAGCAA GGACATCATC 
AAGGAGCTGG AGGAGATTGC CGGCTCGCGA GGCGTCGGCA ATTCCAAACT GAATGTGGAG 
GTCAAAACGG ACCGAAGTTG CCGCTTGATA GCTTTAAAAT CGCTGCTCCA GAAGCTGCCG 
CCCATCAACT TTGCCATACT CAAATACATA TTCCAACACT TTGTGCACGT ATCAGACAAT 
TCGAAGCTGA ATAGCATGGA CAGCAAGAAC TTGGCCATTT GCTGGTGGCC GACACTCATA 
CCCATCGACT TCACCGACAT GGGCCACTTC GAGCAGCTGC GTCCGTATCT GGAGGACATT 
GTCCAGACAA TGATTGACCA GTTCCCGTAT CTGTTCTGCG GCAAGGATGC TTTTGTCATG 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAPp190 ( NM_167573.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728090.1 Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant C, mRNA.

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CloneID	OFa01923
Clone ID Related Accession (Same CDS sequence)	NM_133003.3 , NM_167572.2 , NM_167573.2
Accession Version	NM_167573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein p190, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167573.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAAGT GGCTGGAGGA CAAGAGCGAT 
GGGCGACGCA ACATGAACAA GAATTTGATT TGGAACAACT TCAGCGGATC CACACATGCC 
TACACCACCG GTCGCCGTCA CATCGACTCC AATTTGAACA AAATCCGCCC GAAGGGACCC 
AGTCAAACGC TCAAAGTGGG CGAGGCGCCC AGTCGCAATT GCCCGGCCAT GAGCTCCTCC 
ACCTTCACCC TGCCCACGCA GCAGCCGGGC AAGCTGAACA TGAAGAACTT CCAGCTGGTC 
AGCGATGCGG TGGCCAAGAT GAATTTCACC GGCTCCGGTT CGGGTTCGGG TTCCGGCTCG 
GGATCGGGCA GCACTGGACT GGGCCTGGGT CTGGGCTCCG GCAGTGGCTG CATGGGTGAT 
TCGTTTTTGG AGCCGGTCGA CAAGGACGGC AAGCGCTACG ATCATGCCCA ATTGGATGGC 
GAGGATGAGG ATTCCGAGGA GCTCGCCGAG TACGAGCAGA TCTACGAAAA TGAAGACTGC 
ACCGAATCGG ACAGCTGTGC CAGTTCCACG GAGCGACGGG TGCGCCAGCA GAATGCCTAC 
TATAAGGCCA GCAAGAAGCC GGTGGCCGCC AAGAAGCAGA AAAAGAAGAA GGTGGCAATT 
CCGGTGCAGA CACCGCGTGT TCCTCCGTTC GGCTCGTATG TGAGTCCGCC GGAGATTCCG 
CTGCACTACC AGCGCATGGC CGTTGGCGGC AGCGGGCCAG AGAAACCAGA GCCATGTGTT 
CCCGAGTTCA TGAAGAGTGA CAAGTCGCCA GAGTATTCCA TGGTGCCAGA ACTGGCGGGT 
GCCGGCATCT TCGGTGCAGA GAATCTGCCC GAGTACAACA TGAACCAGGC CAAATGCTTG 
AAGGACTTTG AGAAGCTGGA GAAGCGGCGC ATCAAAGAGG AAACGGCCAG GCAGCGCAAA 
CTACAGGAGA AGGAGAAGGA GCAAGAGAAG AAGCTCAAGC GCAAACTAAA GCAGAATGCC 
AAGGGCCTGG TTGAATCCGC GGAGGCGCAA TTCGGCAAGC TGATGATTAC CTCCGAGCAA 
GGCGAGATCC CCATCTTCCT CAACAAGTGC GTCGAGTTTA TCGAGAAGGA GGGCCTCGAC 
TCCGAGGGCA TTTATAGGGT GCCCGGAAGT AGGGCGCATG TCGACATGTT GTTCCAACGC 
TTCGAAGAGG ATACCAATAC TGAGATTGAT GCGCTCGACA TTCCCGTCAA TGCCGTGGCC 
ACGGCACTGA AGGACTTCTT CTCCAAGCGC CTGCCTCCGC TGTTCAGCAA GGACATCATC 
AAGGAGCTGG AGGAGATTGC CGGCTCGCGA GGCGTCGGCA ATTCCAAACT GAATGTGGAG 
GTCAAAACGG ACCGAAGTTG CCGCTTGATA GCTTTAAAAT CGCTGCTCCA GAAGCTGCCG 
CCCATCAACT TTGCCATACT CAAATACATA TTCCAACACT TTGTGCACGT ATCAGACAAT 
TCGAAGCTGA ATAGCATGGA CAGCAAGAAC TTGGCCATTT GCTGGTGGCC GACACTCATA 
CCCATCGACT TCACCGACAT GGGCCACTTC GAGCAGCTGC GTCCGTATCT GGAGGACATT 
GTCCAGACAA TGATTGACCA GTTCCCGTAT CTGTTCTGCG GCAAGGATGC TTTTGTCATG 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728090.1
CDS	483..5168
Translation	MRQFNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGDVRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEKEYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYELYVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELLDTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNKKLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEAEDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMGRECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQEDSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWKSSGSGSDRTLNLLIVGSEHLASDLLNDIRICTGSKGEYIYENQTYYLNYRIANGDMEAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDKFENLPIVLVYQPQDLKENEVEYLRNEGMRLSEMLHCDFIDHTQNHQKYVYDILNIVILSLKLTEMKSYEPYPSNHTDLRILCCIFCGDQYDIENIVQPLVEESTLVKANEHSIIVDVFIGDAKRRVEFILSSYHGTSQYRDELIHGYIYFYSTKRRSSLANLSILAAQNANIPLQIIAVTESGGVNAFFNSDICQFLITEGNAVADRFKGSFMTFSADQYVKFAFYNPFLKTAWDNKYEVENLHVEESITLDSGEGTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEERDISLDEIYDDNEKPKHLHQKWLEDKSDGRRNMNKNLIWNNFSGSTHAYTTGRRHIDSNLNKIRPKGPSQTLKVGEAPSRNCPAMSSSTFTLPTQQPGKLNMKNFQLVSDAVAKMNFTGSGSGSGSGSGSGSTGLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQIYENEDCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGSGPEKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIPIFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLFCGKDAFVMV

Target ORF information:

RefSeq Version	NM_167573.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167573.2

ORF Insert Sequence:

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAAGT GGCTGGAGGA CAAGAGCGAT 
GGGCGACGCA ACATGAACAA GAATTTGATT TGGAACAACT TCAGCGGATC CACACATGCC 
TACACCACCG GTCGCCGTCA CATCGACTCC AATTTGAACA AAATCCGCCC GAAGGGACCC 
AGTCAAACGC TCAAAGTGGG CGAGGCGCCC AGTCGCAATT GCCCGGCCAT GAGCTCCTCC 
ACCTTCACCC TGCCCACGCA GCAGCCGGGC AAGCTGAACA TGAAGAACTT CCAGCTGGTC 
AGCGATGCGG TGGCCAAGAT GAATTTCACC GGCTCCGGTT CGGGTTCGGG TTCCGGCTCG 
GGATCGGGCA GCACTGGACT GGGCCTGGGT CTGGGCTCCG GCAGTGGCTG CATGGGTGAT 
TCGTTTTTGG AGCCGGTCGA CAAGGACGGC AAGCGCTACG ATCATGCCCA ATTGGATGGC 
GAGGATGAGG ATTCCGAGGA GCTCGCCGAG TACGAGCAGA TCTACGAAAA TGAAGACTGC 
ACCGAATCGG ACAGCTGTGC CAGTTCCACG GAGCGACGGG TGCGCCAGCA GAATGCCTAC 
TATAAGGCCA GCAAGAAGCC GGTGGCCGCC AAGAAGCAGA AAAAGAAGAA GGTGGCAATT 
CCGGTGCAGA CACCGCGTGT TCCTCCGTTC GGCTCGTATG TGAGTCCGCC GGAGATTCCG 
CTGCACTACC AGCGCATGGC CGTTGGCGGC AGCGGGCCAG AGAAACCAGA GCCATGTGTT 
CCCGAGTTCA TGAAGAGTGA CAAGTCGCCA GAGTATTCCA TGGTGCCAGA ACTGGCGGGT 
GCCGGCATCT TCGGTGCAGA GAATCTGCCC GAGTACAACA TGAACCAGGC CAAATGCTTG 
AAGGACTTTG AGAAGCTGGA GAAGCGGCGC ATCAAAGAGG AAACGGCCAG GCAGCGCAAA 
CTACAGGAGA AGGAGAAGGA GCAAGAGAAG AAGCTCAAGC GCAAACTAAA GCAGAATGCC 
AAGGGCCTGG TTGAATCCGC GGAGGCGCAA TTCGGCAAGC TGATGATTAC CTCCGAGCAA 
GGCGAGATCC CCATCTTCCT CAACAAGTGC GTCGAGTTTA TCGAGAAGGA GGGCCTCGAC 
TCCGAGGGCA TTTATAGGGT GCCCGGAAGT AGGGCGCATG TCGACATGTT GTTCCAACGC 
TTCGAAGAGG ATACCAATAC TGAGATTGAT GCGCTCGACA TTCCCGTCAA TGCCGTGGCC 
ACGGCACTGA AGGACTTCTT CTCCAAGCGC CTGCCTCCGC TGTTCAGCAA GGACATCATC 
AAGGAGCTGG AGGAGATTGC CGGCTCGCGA GGCGTCGGCA ATTCCAAACT GAATGTGGAG 
GTCAAAACGG ACCGAAGTTG CCGCTTGATA GCTTTAAAAT CGCTGCTCCA GAAGCTGCCG 
CCCATCAACT TTGCCATACT CAAATACATA TTCCAACACT TTGTGCACGT ATCAGACAAT 
TCGAAGCTGA ATAGCATGGA CAGCAAGAAC TTGGCCATTT GCTGGTGGCC GACACTCATA 
CCCATCGACT TCACCGACAT GGGCCACTTC GAGCAGCTGC GTCCGTATCT GGAGGACATT 
GTCCAGACAA TGATTGACCA GTTCCCGTAT CTGTTCTGCG GCAAGGATGC TTTTGTCATG 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAPp190 ( NM_001272729.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259658.1 Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant F, mRNA.

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CloneID	OFa03701
Clone ID Related Accession (Same CDS sequence)	NM_001272729.1
Accession Version	NM_001272729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein p190, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAGAT TCCAGATATT CCCTCCTCCA 
ACAACTCCTC CAGAGCCAGC CCCTCCAGAT CATCAGCTCA TTACATGCAC ATATAAGAGT 
CAATTCGTTT CGGCTTCAGA ATCAAGTTTG GAAGAAATAA CATCGGATGT CAGCGGGTCC 
AAGGATTCAC TGGCCACCCA TGATGCAGAG TGGCTGGAGG ACAAGAGCGA TGGGCGACGC 
AACATGAACA AGAATTTGAT TTGGAACAAC TTCAGCGGAT CCACACATGC CTACACCACC 
GGTCGCCGTC ACATCGACTC CAATTTGAAC AAAATCCGCC CGAAGGGACC CAGTCAAACG 
CTCAAAGTGG GCGAGGCGCC CAGTCGCAAT TGCCCGGCCA TGAGCTCCTC CACCTTCACC 
CTGCCCACGC AGCAGCCGGG CAAGCTGAAC ATGAAGAACT TCCAGCTGGT CAGCGATGCG 
GTGGCCAAGA TGAATTTCAC CGGCTCCGGT TCGGGTTCGG GTTCCGGCTC GGGATCGGGC 
AGCACTGGAC TGGGCCTGGG TCTGGGCTCC GGCAGTGGCT GCATGGGTGA TTCGTTTTTG 
GAGCCGGTCG ACAAGGACGG CAAGCGCTAC GATCATGCCC AATTGGATGG CGAGGATGAG 
GATTCCGAGG AGCTCGCCGA GTACGAGCAG ATCTACGAAA ATGAAGACTG CACCGAATCG 
GACAGCTGTG CCAGTTCCAC GGAGCGACGG GTGCGCCAGC AGAATGCCTA CTATAAGGCC 
AGCAAGAAGC CGGTGGCCGC CAAGAAGCAG AAAAAGAAGA AGGTGGCAAT TCCGGTGCAG 
ACACCGCGTG TTCCTCCGTT CGGCTCGTAT GTGAGTCCGC CGGAGATTCC GCTGCACTAC 
CAGCGCATGG CCGTTGGCGG CAGCGGGCCA GGTGAGAAAA AGAAACCAGA GCCATGTGTT 
CCCGAGTTCA TGAAGAGTGA CAAGTCGCCA GAGTATTCCA TGGTGCCAGA ACTGGCGGGT 
GCCGGCATCT TCGGTGCAGA GAATCTGCCC GAGTACAACA TGAACCAGGC CAAATGCTTG 
AAGGACTTTG AGAAGCTGGA GAAGCGGCGC ATCAAAGAGG AAACGGCCAG GCAGCGCAAA 
CTACAGGAGA AGGAGAAGGA GCAAGAGAAG AAGCTCAAGC GCAAACTAAA GCAGAATGCC 
AAGGGCCTGG TTGAATCCGC GGAGGCGCAA TTCGGCAAGC TGATGATTAC CTCCGAGCAA 
GGCGAGATCC CCATCTTCCT CAACAAGTGC GTCGAGTTTA TCGAGAAGGA GGGCCTCGAC 
TCCGAGGGCA TTTATAGGGT GCCCGGAAGT AGGGCGCATG TCGACATGTT GTTCCAACGC 
TTCGAAGAGG ATACCAATAC TGAGATTGAT GCGCTCGACA TTCCCGTCAA TGCCGTGGCC 
ACGGCACTGA AGGACTTCTT CTCCAAGCGC CTGCCTCCGC TGTTCAGCAA GGACATCATC 
AAGGAGCTGG AGGAGATTGC CGGCTCGCGA GGCGTCGGCA ATTCCAAACT GAATGTGGAG 
GTCAAAACGG ACCGAAGTTG CCGCTTGATA GCTTTAAAAT CGCTGCTCCA GAAGCTGCCG 
CCCATCAACT TTGCCATACT CAAATACATA TTCCAACACT TTGTGCACGT ATCAGACAAT 
TCGAAGCTGA ATAGCATGGA CAGCAAGAAC TTGGCCATTT GCTGGTGGCC GACACTCATA 
CCCATCGACT TCACCGACAT GGGCCACTTC GAGCAGCTGC GTCCGTATCT GGAGGACATT 
GTCCAGACAA TGATTGACCA GTTCCCGTAT CTGTTCTGCG GCAAGGATGC TTTTGTCATG 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259658.1
CDS	75..4940
Translation	MRQFNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGDVRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEKEYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYELYVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELLDTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNKKLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEAEDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMGRECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQEDSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWKSSGSGSDRTLNLLIVGSEHLASDLLNDIRICTGSKGEYIYENQTYYLNYRIANGDMEAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDKFENLPIVLVYQPQDLKENEVEYLRNEGMRLSEMLHCDFIDHTQNHQKYVYDILNIVILSLKLTEMKSYEPYPSNHTDLRILCCIFCGDQYDIENIVQPLVEESTLVKANEHSIIVDVFIGDAKRRVEFILSSYHGTSQYRDELIHGYIYFYSTKRRSSLANLSILAAQNANIPLQIIAVTESGGVNAFFNSDICQFLITEGNAVADRFKGSFMTFSADQYVKFAFYNPFLKTAWDNKYEVENLHVEESITLDSGEGTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEERDISLDEIYDDNEKPKHLHQRFQIFPPPTTPPEPAPPDHQLITCTYKSQFVSASESSLEEITSDVSGSKDSLATHDAEWLEDKSDGRRNMNKNLIWNNFSGSTHAYTTGRRHIDSNLNKIRPKGPSQTLKVGEAPSRNCPAMSSSTFTLPTQQPGKLNMKNFQLVSDAVAKMNFTGSGSGSGSGSGSGSTGLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQIYENEDCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGSGPGEKKKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIPIFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLFCGKDAFVMV

Target ORF information:

RefSeq Version	NM_001272729.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272729.1

ORF Insert Sequence:

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAGAT TCCAGATATT CCCTCCTCCA 
ACAACTCCTC CAGAGCCAGC CCCTCCAGAT CATCAGCTCA TTACATGCAC ATATAAGAGT 
CAATTCGTTT CGGCTTCAGA ATCAAGTTTG GAAGAAATAA CATCGGATGT CAGCGGGTCC 
AAGGATTCAC TGGCCACCCA TGATGCAGAG TGGCTGGAGG ACAAGAGCGA TGGGCGACGC 
AACATGAACA AGAATTTGAT TTGGAACAAC TTCAGCGGAT CCACACATGC CTACACCACC 
GGTCGCCGTC ACATCGACTC CAATTTGAAC AAAATCCGCC CGAAGGGACC CAGTCAAACG 
CTCAAAGTGG GCGAGGCGCC CAGTCGCAAT TGCCCGGCCA TGAGCTCCTC CACCTTCACC 
CTGCCCACGC AGCAGCCGGG CAAGCTGAAC ATGAAGAACT TCCAGCTGGT CAGCGATGCG 
GTGGCCAAGA TGAATTTCAC CGGCTCCGGT TCGGGTTCGG GTTCCGGCTC GGGATCGGGC 
AGCACTGGAC TGGGCCTGGG TCTGGGCTCC GGCAGTGGCT GCATGGGTGA TTCGTTTTTG 
GAGCCGGTCG ACAAGGACGG CAAGCGCTAC GATCATGCCC AATTGGATGG CGAGGATGAG 
GATTCCGAGG AGCTCGCCGA GTACGAGCAG ATCTACGAAA ATGAAGACTG CACCGAATCG 
GACAGCTGTG CCAGTTCCAC GGAGCGACGG GTGCGCCAGC AGAATGCCTA CTATAAGGCC 
AGCAAGAAGC CGGTGGCCGC CAAGAAGCAG AAAAAGAAGA AGGTGGCAAT TCCGGTGCAG 
ACACCGCGTG TTCCTCCGTT CGGCTCGTAT GTGAGTCCGC CGGAGATTCC GCTGCACTAC 
CAGCGCATGG CCGTTGGCGG CAGCGGGCCA GGTGAGAAAA AGAAACCAGA GCCATGTGTT 
CCCGAGTTCA TGAAGAGTGA CAAGTCGCCA GAGTATTCCA TGGTGCCAGA ACTGGCGGGT 
GCCGGCATCT TCGGTGCAGA GAATCTGCCC GAGTACAACA TGAACCAGGC CAAATGCTTG 
AAGGACTTTG AGAAGCTGGA GAAGCGGCGC ATCAAAGAGG AAACGGCCAG GCAGCGCAAA 
CTACAGGAGA AGGAGAAGGA GCAAGAGAAG AAGCTCAAGC GCAAACTAAA GCAGAATGCC 
AAGGGCCTGG TTGAATCCGC GGAGGCGCAA TTCGGCAAGC TGATGATTAC CTCCGAGCAA 
GGCGAGATCC CCATCTTCCT CAACAAGTGC GTCGAGTTTA TCGAGAAGGA GGGCCTCGAC 
TCCGAGGGCA TTTATAGGGT GCCCGGAAGT AGGGCGCATG TCGACATGTT GTTCCAACGC 
TTCGAAGAGG ATACCAATAC TGAGATTGAT GCGCTCGACA TTCCCGTCAA TGCCGTGGCC 
ACGGCACTGA AGGACTTCTT CTCCAAGCGC CTGCCTCCGC TGTTCAGCAA GGACATCATC 
AAGGAGCTGG AGGAGATTGC CGGCTCGCGA GGCGTCGGCA ATTCCAAACT GAATGTGGAG 
GTCAAAACGG ACCGAAGTTG CCGCTTGATA GCTTTAAAAT CGCTGCTCCA GAAGCTGCCG 
CCCATCAACT TTGCCATACT CAAATACATA TTCCAACACT TTGTGCACGT ATCAGACAAT 
TCGAAGCTGA ATAGCATGGA CAGCAAGAAC TTGGCCATTT GCTGGTGGCC GACACTCATA 
CCCATCGACT TCACCGACAT GGGCCACTTC GAGCAGCTGC GTCCGTATCT GGAGGACATT 
GTCCAGACAA TGATTGACCA GTTCCCGTAT CTGTTCTGCG GCAAGGATGC TTTTGTCATG 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAPp190 ( NM_001272728.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259657.1 Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant E, mRNA.

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CloneID	OFa03702
Clone ID Related Accession (Same CDS sequence)	NM_001272728.1
Accession Version	NM_001272728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein p190, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAACGG ATGTCAGCGG GTCCAAGGAT 
TCACTGGCCA CCCATGATGC AGAGTGGCTG GAGGACAAGA GCGATGGGCG ACGCAACATG 
AACAAGAATT TGATTTGGAA CAACTTCAGC GGATCCACAC ATGCCTACAC CACCGGTCGC 
CGTCACATCG ACTCCAATTT GAACAAAATC CGCCCGAAGG GACCCAGTCA AACGCTCAAA 
GTGGGCGAGG CGCCCAGTCG CAATTGCCCG GCCATGAGCT CCTCCACCTT CACCCTGCCC 
ACGCAGCAGC CGGGCAAGCT GAACATGAAG AACTTCCAGC TGGTCAGCGA TGCGGTGGCC 
AAGATGAATT TCACCGGCTC CGGTTCGGGT TCGGGTTCCG GCTCGGGATC GGGCAGCACT 
GGACTGGGCC TGGGTCTGGG CTCCGGCAGT GGCTGCATGG GTGATTCGTT TTTGGAGCCG 
GTCGACAAGG ACGGCAAGCG CTACGATCAT GCCCAATTGG ATGGCGAGGA TGAGGATTCC 
GAGGAGCTCG CCGAGTACGA GCAGATCTAC GAAAATGAAG ACTGCACCGA ATCGGACAGC 
TGTGCCAGTT CCACGGAGCG ACGGGTGCGC CAGCAGAATG CCTACTATAA GGCCAGCAAG 
AAGCCGGTGG CCGCCAAGAA GCAGAAAAAG AAGAAGGTGG CAATTCCGGT GCAGACACCG 
CGTGTTCCTC CGTTCGGCTC GTATGTGAGT CCGCCGGAGA TTCCGCTGCA CTACCAGCGC 
ATGGCCGTTG GCGGCAGCGG GCCAGGTGAG AAAAAGAAAC CAGAGCCATG TGTTCCCGAG 
TTCATGAAGA GTGACAAGTC GCCAGAGTAT TCCATGGTGC CAGAACTGGC GGGTGCCGGC 
ATCTTCGGTG CAGAGAATCT GCCCGAGTAC AACATGAACC AGGCCAAATG CTTGAAGGAC 
TTTGAGAAGC TGGAGAAGCG GCGCATCAAA GAGGAAACGG CCAGGCAGCG CAAACTACAG 
GAGAAGGAGA AGGAGCAAGA GAAGAAGCTC AAGCGCAAAC TAAAGCAGAA TGCCAAGGGC 
CTGGTTGAAT CCGCGGAGGC GCAATTCGGC AAGCTGATGA TTACCTCCGA GCAAGGCGAG 
ATCCCCATCT TCCTCAACAA GTGCGTCGAG TTTATCGAGA AGGAGGGCCT CGACTCCGAG 
GGCATTTATA GGGTGCCCGG AAGTAGGGCG CATGTCGACA TGTTGTTCCA ACGCTTCGAA 
GAGGATACCA ATACTGAGAT TGATGCGCTC GACATTCCCG TCAATGCCGT GGCCACGGCA 
CTGAAGGACT TCTTCTCCAA GCGCCTGCCT CCGCTGTTCA GCAAGGACAT CATCAAGGAG 
CTGGAGGAGA TTGCCGGCTC GCGAGGCGTC GGCAATTCCA AACTGAATGT GGAGGTCAAA 
ACGGACCGAA GTTGCCGCTT GATAGCTTTA AAATCGCTGC TCCAGAAGCT GCCGCCCATC 
AACTTTGCCA TACTCAAATA CATATTCCAA CACTTTGTGC ACGTATCAGA CAATTCGAAG 
CTGAATAGCA TGGACAGCAA GAACTTGGCC ATTTGCTGGT GGCCGACACT CATACCCATC 
GACTTCACCG ACATGGGCCA CTTCGAGCAG CTGCGTCCGT ATCTGGAGGA CATTGTCCAG 
ACAATGATTG ACCAGTTCCC GTATCTGTTC TGCGGCAAGG ATGCTTTTGT CATGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259657.1
CDS	483..5222
Translation	MRQFNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGDVRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEKEYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYELYVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELLDTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNKKLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEAEDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMGRECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQEDSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWKSSGSGSDRTLNLLIVGSEHLASDLLNDIRICTGSKGEYIYENQTYYLNYRIANGDMEAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDKFENLPIVLVYQPQDLKENEVEYLRNEGMRLSEMLHCDFIDHTQNHQKYVYDILNIVILSLKLTEMKSYEPYPSNHTDLRILCCIFCGDQYDIENIVQPLVEESTLVKANEHSIIVDVFIGDAKRRVEFILSSYHGTSQYRDELIHGYIYFYSTKRRSSLANLSILAAQNANIPLQIIAVTESGGVNAFFNSDICQFLITEGNAVADRFKGSFMTFSADQYVKFAFYNPFLKTAWDNKYEVENLHVEESITLDSGEGTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEERDISLDEIYDDNEKPKHLHQTDVSGSKDSLATHDAEWLEDKSDGRRNMNKNLIWNNFSGSTHAYTTGRRHIDSNLNKIRPKGPSQTLKVGEAPSRNCPAMSSSTFTLPTQQPGKLNMKNFQLVSDAVAKMNFTGSGSGSGSGSGSGSTGLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQIYENEDCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGSGPGEKKKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIPIFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLFCGKDAFVMV

Target ORF information:

RefSeq Version	NM_001272728.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272728.1

ORF Insert Sequence:

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAACGG ATGTCAGCGG GTCCAAGGAT 
TCACTGGCCA CCCATGATGC AGAGTGGCTG GAGGACAAGA GCGATGGGCG ACGCAACATG 
AACAAGAATT TGATTTGGAA CAACTTCAGC GGATCCACAC ATGCCTACAC CACCGGTCGC 
CGTCACATCG ACTCCAATTT GAACAAAATC CGCCCGAAGG GACCCAGTCA AACGCTCAAA 
GTGGGCGAGG CGCCCAGTCG CAATTGCCCG GCCATGAGCT CCTCCACCTT CACCCTGCCC 
ACGCAGCAGC CGGGCAAGCT GAACATGAAG AACTTCCAGC TGGTCAGCGA TGCGGTGGCC 
AAGATGAATT TCACCGGCTC CGGTTCGGGT TCGGGTTCCG GCTCGGGATC GGGCAGCACT 
GGACTGGGCC TGGGTCTGGG CTCCGGCAGT GGCTGCATGG GTGATTCGTT TTTGGAGCCG 
GTCGACAAGG ACGGCAAGCG CTACGATCAT GCCCAATTGG ATGGCGAGGA TGAGGATTCC 
GAGGAGCTCG CCGAGTACGA GCAGATCTAC GAAAATGAAG ACTGCACCGA ATCGGACAGC 
TGTGCCAGTT CCACGGAGCG ACGGGTGCGC CAGCAGAATG CCTACTATAA GGCCAGCAAG 
AAGCCGGTGG CCGCCAAGAA GCAGAAAAAG AAGAAGGTGG CAATTCCGGT GCAGACACCG 
CGTGTTCCTC CGTTCGGCTC GTATGTGAGT CCGCCGGAGA TTCCGCTGCA CTACCAGCGC 
ATGGCCGTTG GCGGCAGCGG GCCAGGTGAG AAAAAGAAAC CAGAGCCATG TGTTCCCGAG 
TTCATGAAGA GTGACAAGTC GCCAGAGTAT TCCATGGTGC CAGAACTGGC GGGTGCCGGC 
ATCTTCGGTG CAGAGAATCT GCCCGAGTAC AACATGAACC AGGCCAAATG CTTGAAGGAC 
TTTGAGAAGC TGGAGAAGCG GCGCATCAAA GAGGAAACGG CCAGGCAGCG CAAACTACAG 
GAGAAGGAGA AGGAGCAAGA GAAGAAGCTC AAGCGCAAAC TAAAGCAGAA TGCCAAGGGC 
CTGGTTGAAT CCGCGGAGGC GCAATTCGGC AAGCTGATGA TTACCTCCGA GCAAGGCGAG 
ATCCCCATCT TCCTCAACAA GTGCGTCGAG TTTATCGAGA AGGAGGGCCT CGACTCCGAG 
GGCATTTATA GGGTGCCCGG AAGTAGGGCG CATGTCGACA TGTTGTTCCA ACGCTTCGAA 
GAGGATACCA ATACTGAGAT TGATGCGCTC GACATTCCCG TCAATGCCGT GGCCACGGCA 
CTGAAGGACT TCTTCTCCAA GCGCCTGCCT CCGCTGTTCA GCAAGGACAT CATCAAGGAG 
CTGGAGGAGA TTGCCGGCTC GCGAGGCGTC GGCAATTCCA AACTGAATGT GGAGGTCAAA 
ACGGACCGAA GTTGCCGCTT GATAGCTTTA AAATCGCTGC TCCAGAAGCT GCCGCCCATC 
AACTTTGCCA TACTCAAATA CATATTCCAA CACTTTGTGC ACGTATCAGA CAATTCGAAG 
CTGAATAGCA TGGACAGCAA GAACTTGGCC ATTTGCTGGT GGCCGACACT CATACCCATC 
GACTTCACCG ACATGGGCCA CTTCGAGCAG CTGCGTCCGT ATCTGGAGGA CATTGTCCAG 
ACAATGATTG ACCAGTTCCC GTATCTGTTC TGCGGCAAGG ATGCTTTTGT CATGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAPp190 ( NM_001272727.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259656.1 Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant D, mRNA.

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CloneID	OFa03703
Clone ID Related Accession (Same CDS sequence)	NM_001272727.1
Accession Version	NM_001272727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein p190, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAAGT GGCTGGAGGA CAAGAGCGAT 
GGGCGACGCA ACATGAACAA GAATTTGATT TGGAACAACT TCAGCGGATC CACACATGCC 
TACACCACCG GTCGCCGTCA CATCGACTCC AATTTGAACA AAATCCGCCC GAAGGGACCC 
AGTCAAACGC TCAAAGTGGG CGAGGCGCCC AGTCGCAATT GCCCGGCCAT GAGCTCCTCC 
ACCTTCACCC TGCCCACGCA GCAGCCGGGC AAGCTGAACA TGAAGAACTT CCAGCTGGTC 
AGCGATGCGG TGGCCAAGAT GAATTTCACC GGCTCCGGTT CGGGTTCGGG TTCCGGCTCG 
GGATCGGGCA GCACTGGACT GGGCCTGGGT CTGGGCTCCG GCAGTGGCTG CATGGGTGAT 
TCGTTTTTGG AGCCGGTCGA CAAGGACGGC AAGCGCTACG ATCATGCCCA ATTGGATGGC 
GAGGATGAGG ATTCCGAGGA GCTCGCCGAG TACGAGCAGA TCTACGAAAA TGAAGACTGC 
ACCGAATCGG ACAGCTGTGC CAGTTCCACG GAGCGACGGG TGCGCCAGCA GAATGCCTAC 
TATAAGGCCA GCAAGAAGCC GGTGGCCGCC AAGAAGCAGA AAAAGAAGAA GGTGGCAATT 
CCGGTGCAGA CACCGCGTGT TCCTCCGTTC GGCTCGTATG TGAGTCCGCC GGAGATTCCG 
CTGCACTACC AGCGCATGGC CGTTGGCGGC AGCGGGCCAG GTGAGAAAAA GAAACCAGAG 
CCATGTGTTC CCGAGTTCAT GAAGAGTGAC AAGTCGCCAG AGTATTCCAT GGTGCCAGAA 
CTGGCGGGTG CCGGCATCTT CGGTGCAGAG AATCTGCCCG AGTACAACAT GAACCAGGCC 
AAATGCTTGA AGGACTTTGA GAAGCTGGAG AAGCGGCGCA TCAAAGAGGA AACGGCCAGG 
CAGCGCAAAC TACAGGAGAA GGAGAAGGAG CAAGAGAAGA AGCTCAAGCG CAAACTAAAG 
CAGAATGCCA AGGGCCTGGT TGAATCCGCG GAGGCGCAAT TCGGCAAGCT GATGATTACC 
TCCGAGCAAG GCGAGATCCC CATCTTCCTC AACAAGTGCG TCGAGTTTAT CGAGAAGGAG 
GGCCTCGACT CCGAGGGCAT TTATAGGGTG CCCGGAAGTA GGGCGCATGT CGACATGTTG 
TTCCAACGCT TCGAAGAGGA TACCAATACT GAGATTGATG CGCTCGACAT TCCCGTCAAT 
GCCGTGGCCA CGGCACTGAA GGACTTCTTC TCCAAGCGCC TGCCTCCGCT GTTCAGCAAG 
GACATCATCA AGGAGCTGGA GGAGATTGCC GGCTCGCGAG GCGTCGGCAA TTCCAAACTG 
AATGTGGAGG TCAAAACGGA CCGAAGTTGC CGCTTGATAG CTTTAAAATC GCTGCTCCAG 
AAGCTGCCGC CCATCAACTT TGCCATACTC AAATACATAT TCCAACACTT TGTGCACGTA 
TCAGACAATT CGAAGCTGAA TAGCATGGAC AGCAAGAACT TGGCCATTTG CTGGTGGCCG 
ACACTCATAC CCATCGACTT CACCGACATG GGCCACTTCG AGCAGCTGCG TCCGTATCTG 
GAGGACATTG TCCAGACAAT GATTGACCAG TTCCCGTATC TGTTCTGCGG CAAGGATGCT 
TTTGTCATGG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259656.1
CDS	136..4830
Translation	MRQFNISVIGLSGTEKDRGQVGVGKSCLCNRFMRPMADDYFIDHISVLSQSDFSGRIVNNDHFLYWGDVRKTTEEGVEYQFNIIEQTEFMDDSTFQAFKVGKMDPYSKRCTATKVFSAEKLMYICKNQLGIEKEYEQKVMPDGRLSIDGFVVVFDVSPVPNRSVEKQVEFVQNVIATILKNKKPLVLVTTKNDDAYELYVREAEKISQRKDYKSTVQLIETSAHESINIDLAFLLLAQMIDKVKNRVKIISYQESAKSRKELLDTRSEAVTRLIRNQITDYHVLWSQGSKMLSQYREWNEFLNIFGHEAGQKLFRRHMKKLRDDHLNKKLHQYLDKFALALEYLLPDIGALNISDDDAWECARNYLQNHIEFEQYFFECPQASWTELVDMDEAEDEARIPFDVLETSEAETVFRNYLNSVQQDKKKIGWKQQFKMLLEESGFVTPGKQLSEVRVLFMGRECFEALSEHDCQQIYDIHQDDIIEKSKQNFVELLLEHAQYFLQFKNVDNITQEDVRQITDVIQEDSRYKMLDRLDQERRLMLVQHLRFIHCPIRDHCPFFYNCVDSLIEEVLSDKSASNHKTPSGGGWKSSGSGSDRTLNLLIVGSEHLASDLLNDIRICTGSKGEYIYENQTYYLNYRIANGDMEAFKAIDVYSSGLICVYSNQQSFETLKDNLERTLLCNLELEDKFENLPIVLVYQPQDLKENEVEYLRNEGMRLSEMLHCDFIDHTQNHQKYVYDILNIVILSLKLTEMKSYEPYPSNHTDLRILCCIFCGDQYDIENIVQPLVEESTLVKANEHSIIVDVFIGDAKRRVEFILSSYHGTSQYRDELIHGYIYFYSTKRRSSLANLSILAAQNANIPLQIIAVTESGGVNAFFNSDICQFLITEGNAVADRFKGSFMTFSADQYVKFAFYNPFLKTAWDNKYEVENLHVEESITLDSGEGTLENSVNQMPRPPPRHESYMLSNTLGTDGSGSENYEMAPTRSLNSLNEERDISLDEIYDDNEKPKHLHQKWLEDKSDGRRNMNKNLIWNNFSGSTHAYTTGRRHIDSNLNKIRPKGPSQTLKVGEAPSRNCPAMSSSTFTLPTQQPGKLNMKNFQLVSDAVAKMNFTGSGSGSGSGSGSGSTGLGLGLGSGSGCMGDSFLEPVDKDGKRYDHAQLDGEDEDSEELAEYEQIYENEDCTESDSCASSTERRVRQQNAYYKASKKPVAAKKQKKKKVAIPVQTPRVPPFGSYVSPPEIPLHYQRMAVGGSGPGEKKKPEPCVPEFMKSDKSPEYSMVPELAGAGIFGAENLPEYNMNQAKCLKDFEKLEKRRIKEETARQRKLQEKEKEQEKKLKRKLKQNAKGLVESAEAQFGKLMITSEQGEIPIFLNKCVEFIEKEGLDSEGIYRVPGSRAHVDMLFQRFEEDTNTEIDALDIPVNAVATALKDFFSKRLPPLFSKDIIKELEEIAGSRGVGNSKLNVEVKTDRSCRLIALKSLLQKLPPINFAILKYIFQHFVHVSDNSKLNSMDSKNLAICWWPTLIPIDFTDMGHFEQLRPYLEDIVQTMIDQFPYLFCGKDAFVMV

Target ORF information:

RefSeq Version	NM_001272727.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein p190 (RhoGAPp190), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272727.1

ORF Insert Sequence:

ATGCGTCAGT TTAACATCTC GGTCATTGGA CTATCCGGGA CCGAAAAGGA CCGCGGGCAG 
GTGGGAGTGG GCAAGTCATG CCTGTGCAAC AGATTCATGC GCCCGATGGC CGACGACTAC 
TTCATCGATC ACATATCAGT GCTTAGCCAA AGCGACTTTA GTGGCCGGAT CGTGAACAAC 
GACCACTTCC TTTACTGGGG CGATGTGCGC AAGACGACGG AGGAGGGCGT CGAGTACCAG 
TTTAATATCA TCGAGCAGAC GGAGTTCATG GACGACTCCA CGTTCCAAGC CTTTAAGGTG 
GGAAAGATGG ATCCATACTC AAAGCGGTGC ACGGCCACCA AGGTCTTCTC CGCGGAGAAG 
CTAATGTACA TATGCAAGAA TCAGTTGGGC ATCGAGAAGG AGTACGAACA GAAAGTCATG 
CCCGATGGCC GGCTCAGTAT CGATGGCTTT GTTGTCGTCT TCGACGTTAG TCCAGTGCCC 
AATCGCAGTG TGGAGAAGCA GGTGGAGTTC GTGCAGAATG TCATCGCAAC CATACTAAAG 
AACAAGAAGC CACTGGTGCT GGTGACCACA AAGAACGACG ACGCTTATGA GTTGTATGTT 
CGAGAGGCGG AGAAAATTAG CCAGCGAAAG GACTACAAGA GCACTGTGCA GCTAATCGAG 
ACGTCGGCCC ACGAGAGCAT CAACATCGAT TTGGCATTCC TCCTGCTCGC CCAGATGATC 
GACAAGGTTA AGAACCGTGT CAAGATCATC TCCTACCAGG AGTCGGCCAA GTCACGCAAA 
GAACTGCTGG ACACACGATC CGAGGCGGTG ACGCGACTAA TACGCAACCA GATCACCGAC 
TATCACGTCC TGTGGTCGCA GGGCTCCAAG ATGCTGTCAC AGTATCGCGA GTGGAACGAG 
TTCCTCAACA TATTCGGCCA CGAGGCCGGC CAAAAGCTAT TCCGGCGGCA CATGAAGAAG 
CTGCGCGACG ATCATCTGAA CAAAAAACTG CATCAGTACT TGGATAAGTT TGCCCTGGCC 
CTGGAGTACC TGCTGCCGGA CATCGGGGCT TTGAATATCA GCGATGACGA TGCTTGGGAG 
TGCGCCAGGA ACTATCTGCA GAATCACATC GAGTTCGAGC AGTACTTCTT CGAGTGCCCG 
CAGGCCTCGT GGACGGAGCT GGTGGACATG GACGAGGCGG AGGATGAGGC CCGCATTCCC 
TTTGATGTGC TAGAGACTTC CGAGGCAGAG ACTGTCTTCC GTAACTACTT GAACTCGGTG 
CAGCAGGACA AGAAAAAAAT TGGATGGAAG CAGCAATTCA AAATGCTACT TGAGGAGTCG 
GGCTTTGTGA CGCCCGGCAA GCAGCTGTCG GAGGTGCGCG TCCTCTTCAT GGGACGCGAA 
TGCTTTGAGG CGCTTTCGGA GCACGACTGC CAGCAGATCT ACGACATTCA CCAGGACGAC 
ATCATTGAAA AGAGCAAACA GAATTTTGTG GAGCTGCTGC TGGAACATGC TCAATATTTT 
CTACAGTTCA AGAACGTTGA CAACATCACT CAGGAAGATG TGCGCCAGAT AACCGATGTC 
ATACAGGAGG ATTCACGCTA CAAGATGCTC GATCGCTTGG ACCAGGAGCG GCGGTTGATG 
CTTGTCCAGC ACCTGCGCTT CATCCACTGC CCCATCCGCG ATCACTGCCC ATTCTTTTAC 
AACTGTGTGG ATAGCCTGAT TGAGGAGGTG TTGTCCGACA AGTCGGCCAG CAACCACAAG 
ACGCCCAGCG GCGGTGGCTG GAAGAGTTCC GGCAGCGGCA GTGACCGCAC GCTCAATCTT 
CTGATCGTGG GCTCCGAGCA CCTGGCCAGC GATCTGCTCA ACGACATCCG CATATGTACC 
GGTAGCAAGG GCGAGTACAT CTACGAGAAC CAGACGTATT ATCTCAATTA CCGAATCGCC 
AACGGCGACA TGGAGGCCTT CAAGGCCATA GATGTCTACT CGAGTGGTCT TATTTGCGTG 
TACTCCAATC AGCAATCTTT TGAGACGCTC AAGGATAACT TGGAGCGCAC GTTGCTCTGC 
AACCTGGAGC TGGAGGACAA GTTCGAGAAT CTGCCGATCG TGCTGGTTTA CCAGCCACAG 
GATCTCAAGG AGAACGAGGT GGAATACTTG CGCAACGAGG GCATGCGCCT GTCGGAGATG 
CTGCACTGCG ACTTCATCGA TCATACGCAA AATCATCAGA AGTATGTCTA TGATATACTT 
AACATAGTCA TCCTGTCGCT AAAGCTGACT GAGATGAAGA GCTACGAGCC GTATCCCTCC 
AATCACACCG ACCTGCGCAT CCTGTGCTGC ATCTTTTGTG GCGATCAGTA TGACATCGAG 
AACATTGTCC AGCCGCTGGT GGAGGAATCG ACGCTGGTCA AGGCCAACGA GCACTCCATC 
ATTGTCGACG TCTTTATTGG CGATGCCAAG CGACGGGTCG AGTTTATACT TTCCTCATAT 
CATGGCACTA GCCAGTACCG CGATGAACTA ATCCATGGCT ATATTTACTT TTACTCGACC 
AAGCGTCGAT CTTCGTTGGC AAATCTAAGC ATCCTGGCAG CCCAGAATGC CAATATTCCA 
TTGCAGATTA TCGCGGTGAC CGAGAGCGGG GGCGTTAATG CGTTCTTCAA CAGCGATATT 
TGCCAGTTTC TGATCACCGA GGGCAATGCG GTGGCCGATC GCTTCAAGGG CAGCTTTATG 
ACCTTCTCGG CGGATCAATA TGTCAAGTTT GCGTTCTATA ATCCATTCCT GAAGACAGCC 
TGGGACAACA AGTACGAGGT GGAGAACCTG CATGTGGAGG AGTCAATTAC TTTGGATTCG 
GGCGAGGGCA CGCTGGAGAA CTCGGTTAAT CAGATGCCAC GCCCGCCGCC GCGCCACGAG 
AGCTACATGC TGTCCAATAC GCTGGGAACC GACGGTTCCG GCAGTGAGAA TTACGAAATG 
GCTCCAACCC GATCTCTCAA CTCATTAAAT GAAGAAAGAG ATATATCATT AGATGAAATC 
TACGATGACA ACGAAAAGCC GAAGCACCTG CATCAAAAGT GGCTGGAGGA CAAGAGCGAT 
GGGCGACGCA ACATGAACAA GAATTTGATT TGGAACAACT TCAGCGGATC CACACATGCC 
TACACCACCG GTCGCCGTCA CATCGACTCC AATTTGAACA AAATCCGCCC GAAGGGACCC 
AGTCAAACGC TCAAAGTGGG CGAGGCGCCC AGTCGCAATT GCCCGGCCAT GAGCTCCTCC 
ACCTTCACCC TGCCCACGCA GCAGCCGGGC AAGCTGAACA TGAAGAACTT CCAGCTGGTC 
AGCGATGCGG TGGCCAAGAT GAATTTCACC GGCTCCGGTT CGGGTTCGGG TTCCGGCTCG 
GGATCGGGCA GCACTGGACT GGGCCTGGGT CTGGGCTCCG GCAGTGGCTG CATGGGTGAT 
TCGTTTTTGG AGCCGGTCGA CAAGGACGGC AAGCGCTACG ATCATGCCCA ATTGGATGGC 
GAGGATGAGG ATTCCGAGGA GCTCGCCGAG TACGAGCAGA TCTACGAAAA TGAAGACTGC 
ACCGAATCGG ACAGCTGTGC CAGTTCCACG GAGCGACGGG TGCGCCAGCA GAATGCCTAC 
TATAAGGCCA GCAAGAAGCC GGTGGCCGCC AAGAAGCAGA AAAAGAAGAA GGTGGCAATT 
CCGGTGCAGA CACCGCGTGT TCCTCCGTTC GGCTCGTATG TGAGTCCGCC GGAGATTCCG 
CTGCACTACC AGCGCATGGC CGTTGGCGGC AGCGGGCCAG GTGAGAAAAA GAAACCAGAG 
CCATGTGTTC CCGAGTTCAT GAAGAGTGAC AAGTCGCCAG AGTATTCCAT GGTGCCAGAA 
CTGGCGGGTG CCGGCATCTT CGGTGCAGAG AATCTGCCCG AGTACAACAT GAACCAGGCC 
AAATGCTTGA AGGACTTTGA GAAGCTGGAG AAGCGGCGCA TCAAAGAGGA AACGGCCAGG 
CAGCGCAAAC TACAGGAGAA GGAGAAGGAG CAAGAGAAGA AGCTCAAGCG CAAACTAAAG 
CAGAATGCCA AGGGCCTGGT TGAATCCGCG GAGGCGCAAT TCGGCAAGCT GATGATTACC 
TCCGAGCAAG GCGAGATCCC CATCTTCCTC AACAAGTGCG TCGAGTTTAT CGAGAAGGAG 
GGCCTCGACT CCGAGGGCAT TTATAGGGTG CCCGGAAGTA GGGCGCATGT CGACATGTTG 
TTCCAACGCT TCGAAGAGGA TACCAATACT GAGATTGATG CGCTCGACAT TCCCGTCAAT 
GCCGTGGCCA CGGCACTGAA GGACTTCTTC TCCAAGCGCC TGCCTCCGCT GTTCAGCAAG 
GACATCATCA AGGAGCTGGA GGAGATTGCC GGCTCGCGAG GCGTCGGCAA TTCCAAACTG 
AATGTGGAGG TCAAAACGGA CCGAAGTTGC CGCTTGATAG CTTTAAAATC GCTGCTCCAG 
AAGCTGCCGC CCATCAACTT TGCCATACTC AAATACATAT TCCAACACTT TGTGCACGTA 
TCAGACAATT CGAAGCTGAA TAGCATGGAC AGCAAGAACT TGGCCATTTG CTGGTGGCCG 
ACACTCATAC CCATCGACTT CACCGACATG GGCCACTTCG AGCAGCTGCG TCCGTATCTG 
GAGGACATTG TCCAGACAAT GATTGACCAG TTCCCGTATC TGTTCTGCGG CAAGGATGCT 
TTTGTCATGG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGAPp190 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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