Arhgap35 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arhgap35
Entrez Gene ID	306400
Full Name	Rho GTPase activating protein 35
Synonyms	Grlf1,p190RhoGAP
General protein information	Preferred Names Rho GTPase activating protein 35 Names rho GTPase-activating protein 35 GAP-associated protein p190 glucocorticoid receptor DNA-binding factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271132.1	NP_001258061.1	rho GTPase-activating protein 35
XM_008758919.1	XP_008757141.1	rho GTPase-activating protein 35 isoform X1
XM_006228412.3	XP_006228474.1	rho GTPase-activating protein 35 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGAP35	XP_003316510.1
Macaca mulatta (Rhesus monkey)	GRLF1	XP_001112719.1
Rattus norvegicus (Norway rat)	Arhgap35	NP_001258061.1
Gallus gallus (chicken)	ARHGAP35	XP_003643808.2
Homo sapiens (human)	ARHGAP35	NP_004482.4
Bos taurus (cattle)	ARHGAP35	NP_001179327.1
Danio rerio (zebrafish)	arhgap35	XP_003198691.2
Danio rerio (zebrafish)	LOC100004299	XP_001343636.1
Xenopus tropicalis (tropical clawed frog)	arhgap35	XP_004917009.1
Drosophila melanogaster (fruit fly)	RhoGAPp190	NP_001259658.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000870	XP_316844.5
Mus musculus (house mouse)	Grlf1	NP_766327.3
Canis lupus familiaris (dog)	ARHGAP35	NP_001003022.1

Gene Ontology
Bibliography

Related articles in PubMed

The N-Terminal GTPase Domain of p190RhoGAP Proteins Is a PseudoGTPase.
Stiegler AL, Boggon TJ
Structure (London, England : 1993)26(11)1451-1461.e4(2018 11)

Symmetry breaking in spreading RAT2 fibroblasts requires the MAPK/ERK pathway scaffold RACK1 that integrates FAK, p190A-RhoGAP and ERK2 signaling.
Klímová Z, Bráborec V, Maninová M, Čáslavský J, Weber MJ, Vomastek T
Biochimica et biophysica acta1863(9)2189-200(2016 09)

Cancer-associated mutations in the protrusion-targeting region of p190RhoGAP impact tumor cell migration.
Binamé F, Bidaud-Meynard A, Magnan L, Piquet L, Montibus B, Chabadel A, Saltel F, Lagrée V, Moreau V
The Journal of cell biology214(7)859-73(2016 09)

A Point Mutation in p190A RhoGAP Affects Ciliogenesis and Leads to Glomerulocystic Kidney Defects.
Stewart K, Gaitan Y, Shafer ME, Aoudjit L, Hu D, Sharma R, Tremblay M, Ishii H, Marcotte M, Stanga D, Tang YC, Boualia SK, Nguyen AH, Takano T, Lamarche-Vane N, Vidal S, Bouchard M
PLoS genetics12(2)e1005785(2016 Feb)

Integrin α3 is required for late postnatal stability of dendrite arbors, dendritic spines and synapses, and mouse behavior.
Kerrisk ME, Greer CA, Koleske AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience33(16)6742-52(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Arhgap35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgap35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10371	NM_001271132.1 Latest version!	Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa10371	XM_006228412.3 Latest version!	Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa10371	XM_008758919.1 Latest version!	Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arhgap35 ( NM_001271132.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258061.1 Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), mRNA.

Price & Availability↑

CloneID	ORa10371
Clone ID Related Accession (Same CDS sequence)	NM_001271132.1 , XM_008758919.1 , XM_006228412.3
Accession Version	NM_001271132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	rho GTPase-activating protein 35
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07002648.1 and AABR07002647.1. On Sep 1, 2012 this sequence version replaced XM_001053554.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906353, SAMN01906354 [ECO:0000348] ##Evidence-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCCGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTATGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTCAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCATTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGACTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTAGGG 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TCGATGTTAG CAGGGGCATG AACAGGAACT TCGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTAAT AGTCCTGACC 
AAGTGTGATG AGGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CGAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTG 
GTGAGCCGCA TTGTGAAAAG TCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAT CTGGAAGGGA CACAGAAAGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGCCGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTAGACCAC 
CTGAGCTGCA TTAAAGCAAA AAAGCTGTTA GAGACTAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAGACACC ATGGGATGAA ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAATGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG GGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTATTCA 
GAATTGTTTT ATGAGCTGGA GCTGGATGCT AAACCCAGTA AGGAAAAGAT GGGTGTTATC 
CAGGATGTTC TGGGTGAAGA GCAACGATTT AAAGCATTGC AGAAACTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCA 
AGCTGTCCAG CTTGCGTAGA TGCTAAGATT GAACATTTGA TCAGTTCTCG CTTTATCCGA 
CCATCTGATA GGAATCAGAA GAACTCTTTG TCTGACCCCA ATATTGATAG GATCAATTTG 
GTTATTTTAG GCAAAGATGG CCTTGCCCGA GAGTTAGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TTCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCG CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAAT CTACACTGGG CAGGCGGGAT AATCACTTAG TCCACCTCCC CTTGACTTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT AATACAGCAA 
GGCCAGCAGA TTGCTAGCAA ACTTCAGTGT GTCTTTCTTG ATCCTGCGTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA CGAGAAGCAG ATCAGTCAAG TTCTGAAGGG ACTCCTGGAC 
TCTAAGCGCA ACTTAAACCT GGTTAGTTCT ACTGCTAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTCATGTGT GGTGATCCTT TTAGTGCAGA TGACATTCTC 
TCTCCTGTCC TGCAGTCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG AGTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTGGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AAATTGATGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTCGAGCTCT TCCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATCGAGGCC ACACACATGT ACGATAATGT GGCTGAGGCC 
TGCAGCACCA CTGAGGAGGT ATTCAACTCC CCCAGGGCTG GGTCACCCCT CTGCAATTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCGT ACCACCTTTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CAATGGAGCT GGAGGGAAAC 
GACGGGCTGT CTTTCATAAT GAGCAACTTT GAGAGTAAAC TGAACAACAA AGTACCTCCA 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GAGATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGTCATC GGGAGGGACA GAGGAAGTCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGATTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCAGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGCAATGGTT CTGACAGTGA GATGGACACA 
AGCTCTCTAG AGCGAGGCCG CAAAGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACG 
AGATGCACCC GCCTGGGGCG GTTTGCTAGT TACCGCACCA GCTTCAGTGT TGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCTTA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGGA GGAACACCAA GAAACCAAAG CCCAAACCCC GACCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCT TTAACAACAG TGGTGACTCC AGAGAAGCCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATTGAAG CCACAGGACT AAGCACAGAA 
GGCATCTACC GGGTCAGCGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGATCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCGGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAGAT CAACGACAGG GAGCAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCTAAGGA GAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AGAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGT TGATGCGGCC TGACTTCAGC AGCATGGACG CACTCACAGC CACTCGATCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCG 
ATCAGTGAGC CACCGGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCACC CACTGTCCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTCTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258061.1
CDS	691..5190
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVIVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKSHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDETSHIDNMENERIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILSPVLQSQTCKSSHCGSSNSVLLELPIGVHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNRVSHNNKVNLMTSENLSICFWPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQPSPPQSPPPTPQSPMQPLLSSQLQAEHTL

Target ORF information:

RefSeq Version	NM_001271132.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271132.1

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCCGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTATGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTCAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCATTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGACTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTAGGG 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TCGATGTTAG CAGGGGCATG AACAGGAACT TCGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTAAT AGTCCTGACC 
AAGTGTGATG AGGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CGAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTG 
GTGAGCCGCA TTGTGAAAAG TCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAT CTGGAAGGGA CACAGAAAGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGCCGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTAGACCAC 
CTGAGCTGCA TTAAAGCAAA AAAGCTGTTA GAGACTAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAGACACC ATGGGATGAA ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAATGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG GGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTATTCA 
GAATTGTTTT ATGAGCTGGA GCTGGATGCT AAACCCAGTA AGGAAAAGAT GGGTGTTATC 
CAGGATGTTC TGGGTGAAGA GCAACGATTT AAAGCATTGC AGAAACTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCA 
AGCTGTCCAG CTTGCGTAGA TGCTAAGATT GAACATTTGA TCAGTTCTCG CTTTATCCGA 
CCATCTGATA GGAATCAGAA GAACTCTTTG TCTGACCCCA ATATTGATAG GATCAATTTG 
GTTATTTTAG GCAAAGATGG CCTTGCCCGA GAGTTAGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TTCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCG CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAAT CTACACTGGG CAGGCGGGAT AATCACTTAG TCCACCTCCC CTTGACTTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT AATACAGCAA 
GGCCAGCAGA TTGCTAGCAA ACTTCAGTGT GTCTTTCTTG ATCCTGCGTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA CGAGAAGCAG ATCAGTCAAG TTCTGAAGGG ACTCCTGGAC 
TCTAAGCGCA ACTTAAACCT GGTTAGTTCT ACTGCTAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTCATGTGT GGTGATCCTT TTAGTGCAGA TGACATTCTC 
TCTCCTGTCC TGCAGTCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG AGTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTGGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AAATTGATGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTCGAGCTCT TCCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATCGAGGCC ACACACATGT ACGATAATGT GGCTGAGGCC 
TGCAGCACCA CTGAGGAGGT ATTCAACTCC CCCAGGGCTG GGTCACCCCT CTGCAATTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCGT ACCACCTTTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CAATGGAGCT GGAGGGAAAC 
GACGGGCTGT CTTTCATAAT GAGCAACTTT GAGAGTAAAC TGAACAACAA AGTACCTCCA 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GAGATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGTCATC GGGAGGGACA GAGGAAGTCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGATTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCAGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGCAATGGTT CTGACAGTGA GATGGACACA 
AGCTCTCTAG AGCGAGGCCG CAAAGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACG 
AGATGCACCC GCCTGGGGCG GTTTGCTAGT TACCGCACCA GCTTCAGTGT TGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCTTA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGGA GGAACACCAA GAAACCAAAG CCCAAACCCC GACCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCT TTAACAACAG TGGTGACTCC AGAGAAGCCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATTGAAG CCACAGGACT AAGCACAGAA 
GGCATCTACC GGGTCAGCGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGATCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCGGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAGAT CAACGACAGG GAGCAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCTAAGGA GAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AGAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGT TGATGCGGCC TGACTTCAGC AGCATGGACG CACTCACAGC CACTCGATCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCG 
ATCAGTGAGC CACCGGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCACC CACTGTCCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTCTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap35 ( XM_008758919.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757141.1 Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10371
Clone ID Related Accession (Same CDS sequence)	NM_001271132.1 , XM_008758919.1 , XM_006228412.3
Accession Version	XM_008758919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCCGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTATGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTCAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCATTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGACTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTAGGG 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TCGATGTTAG CAGGGGCATG AACAGGAACT TCGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTAAT AGTCCTGACC 
AAGTGTGATG AGGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CGAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTG 
GTGAGCCGCA TTGTGAAAAG TCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAT CTGGAAGGGA CACAGAAAGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGCCGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTAGACCAC 
CTGAGCTGCA TTAAAGCAAA AAAGCTGTTA GAGACTAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAGACACC ATGGGATGAA ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAATGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG GGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTATTCA 
GAATTGTTTT ATGAGCTGGA GCTGGATGCT AAACCCAGTA AGGAAAAGAT GGGTGTTATC 
CAGGATGTTC TGGGTGAAGA GCAACGATTT AAAGCATTGC AGAAACTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCA 
AGCTGTCCAG CTTGCGTAGA TGCTAAGATT GAACATTTGA TCAGTTCTCG CTTTATCCGA 
CCATCTGATA GGAATCAGAA GAACTCTTTG TCTGACCCCA ATATTGATAG GATCAATTTG 
GTTATTTTAG GCAAAGATGG CCTTGCCCGA GAGTTAGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TTCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCG CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAAT CTACACTGGG CAGGCGGGAT AATCACTTAG TCCACCTCCC CTTGACTTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT AATACAGCAA 
GGCCAGCAGA TTGCTAGCAA ACTTCAGTGT GTCTTTCTTG ATCCTGCGTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA CGAGAAGCAG ATCAGTCAAG TTCTGAAGGG ACTCCTGGAC 
TCTAAGCGCA ACTTAAACCT GGTTAGTTCT ACTGCTAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTCATGTGT GGTGATCCTT TTAGTGCAGA TGACATTCTC 
TCTCCTGTCC TGCAGTCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG AGTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTGGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AAATTGATGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTCGAGCTCT TCCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATCGAGGCC ACACACATGT ACGATAATGT GGCTGAGGCC 
TGCAGCACCA CTGAGGAGGT ATTCAACTCC CCCAGGGCTG GGTCACCCCT CTGCAATTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCGT ACCACCTTTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CAATGGAGCT GGAGGGAAAC 
GACGGGCTGT CTTTCATAAT GAGCAACTTT GAGAGTAAAC TGAACAACAA AGTACCTCCA 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GAGATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGTCATC GGGAGGGACA GAGGAAGTCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGATTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCAGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGCAATGGTT CTGACAGTGA GATGGACACA 
AGCTCTCTAG AGCGAGGCCG CAAAGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACG 
AGATGCACCC GCCTGGGGCG GTTTGCTAGT TACCGCACCA GCTTCAGTGT TGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCTTA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGGA GGAACACCAA GAAACCAAAG CCCAAACCCC GACCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCT TTAACAACAG TGGTGACTCC AGAGAAGCCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATTGAAG CCACAGGACT AAGCACAGAA 
GGCATCTACC GGGTCAGCGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGATCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCGGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAGAT CAACGACAGG GAGCAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCTAAGGA GAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AGAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGT TGATGCGGCC TGACTTCAGC AGCATGGACG CACTCACAGC CACTCGATCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCG 
ATCAGTGAGC CACCGGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCACC CACTGTCCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTCTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757141.1
CDS	309..4808
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVIVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKSHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDETSHIDNMENERIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILSPVLQSQTCKSSHCGSSNSVLLELPIGVHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNRVSHNNKVNLMTSENLSICFWPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQPSPPQSPPPTPQSPMQPLLSSQLQAEHTL

Target ORF information:

RefSeq Version	XM_008758919.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008758919.1

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCCGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTATGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTCAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCATTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGACTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTAGGG 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TCGATGTTAG CAGGGGCATG AACAGGAACT TCGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTAAT AGTCCTGACC 
AAGTGTGATG AGGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CGAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTG 
GTGAGCCGCA TTGTGAAAAG TCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAT CTGGAAGGGA CACAGAAAGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGCCGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTAGACCAC 
CTGAGCTGCA TTAAAGCAAA AAAGCTGTTA GAGACTAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAGACACC ATGGGATGAA ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAATGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG GGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTATTCA 
GAATTGTTTT ATGAGCTGGA GCTGGATGCT AAACCCAGTA AGGAAAAGAT GGGTGTTATC 
CAGGATGTTC TGGGTGAAGA GCAACGATTT AAAGCATTGC AGAAACTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCA 
AGCTGTCCAG CTTGCGTAGA TGCTAAGATT GAACATTTGA TCAGTTCTCG CTTTATCCGA 
CCATCTGATA GGAATCAGAA GAACTCTTTG TCTGACCCCA ATATTGATAG GATCAATTTG 
GTTATTTTAG GCAAAGATGG CCTTGCCCGA GAGTTAGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TTCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCG CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAAT CTACACTGGG CAGGCGGGAT AATCACTTAG TCCACCTCCC CTTGACTTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT AATACAGCAA 
GGCCAGCAGA TTGCTAGCAA ACTTCAGTGT GTCTTTCTTG ATCCTGCGTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA CGAGAAGCAG ATCAGTCAAG TTCTGAAGGG ACTCCTGGAC 
TCTAAGCGCA ACTTAAACCT GGTTAGTTCT ACTGCTAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTCATGTGT GGTGATCCTT TTAGTGCAGA TGACATTCTC 
TCTCCTGTCC TGCAGTCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG AGTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTGGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AAATTGATGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTCGAGCTCT TCCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATCGAGGCC ACACACATGT ACGATAATGT GGCTGAGGCC 
TGCAGCACCA CTGAGGAGGT ATTCAACTCC CCCAGGGCTG GGTCACCCCT CTGCAATTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCGT ACCACCTTTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CAATGGAGCT GGAGGGAAAC 
GACGGGCTGT CTTTCATAAT GAGCAACTTT GAGAGTAAAC TGAACAACAA AGTACCTCCA 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GAGATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGTCATC GGGAGGGACA GAGGAAGTCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGATTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCAGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGCAATGGTT CTGACAGTGA GATGGACACA 
AGCTCTCTAG AGCGAGGCCG CAAAGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACG 
AGATGCACCC GCCTGGGGCG GTTTGCTAGT TACCGCACCA GCTTCAGTGT TGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCTTA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGGA GGAACACCAA GAAACCAAAG CCCAAACCCC GACCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCT TTAACAACAG TGGTGACTCC AGAGAAGCCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATTGAAG CCACAGGACT AAGCACAGAA 
GGCATCTACC GGGTCAGCGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGATCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCGGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAGAT CAACGACAGG GAGCAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCTAAGGA GAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AGAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGT TGATGCGGCC TGACTTCAGC AGCATGGACG CACTCACAGC CACTCGATCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCG 
ATCAGTGAGC CACCGGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCACC CACTGTCCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTCTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap35 ( XM_006228412.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228474.1 Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.

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CloneID	ORa10371
Clone ID Related Accession (Same CDS sequence)	NM_001271132.1 , XM_008758919.1 , XM_006228412.3
Accession Version	XM_006228412.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228412.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCCGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTATGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTCAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCATTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGACTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTAGGG 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TCGATGTTAG CAGGGGCATG AACAGGAACT TCGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTAAT AGTCCTGACC 
AAGTGTGATG AGGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CGAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTG 
GTGAGCCGCA TTGTGAAAAG TCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAT CTGGAAGGGA CACAGAAAGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGCCGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTAGACCAC 
CTGAGCTGCA TTAAAGCAAA AAAGCTGTTA GAGACTAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAGACACC ATGGGATGAA ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAATGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG GGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTATTCA 
GAATTGTTTT ATGAGCTGGA GCTGGATGCT AAACCCAGTA AGGAAAAGAT GGGTGTTATC 
CAGGATGTTC TGGGTGAAGA GCAACGATTT AAAGCATTGC AGAAACTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCA 
AGCTGTCCAG CTTGCGTAGA TGCTAAGATT GAACATTTGA TCAGTTCTCG CTTTATCCGA 
CCATCTGATA GGAATCAGAA GAACTCTTTG TCTGACCCCA ATATTGATAG GATCAATTTG 
GTTATTTTAG GCAAAGATGG CCTTGCCCGA GAGTTAGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TTCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCG CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAAT CTACACTGGG CAGGCGGGAT AATCACTTAG TCCACCTCCC CTTGACTTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT AATACAGCAA 
GGCCAGCAGA TTGCTAGCAA ACTTCAGTGT GTCTTTCTTG ATCCTGCGTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA CGAGAAGCAG ATCAGTCAAG TTCTGAAGGG ACTCCTGGAC 
TCTAAGCGCA ACTTAAACCT GGTTAGTTCT ACTGCTAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTCATGTGT GGTGATCCTT TTAGTGCAGA TGACATTCTC 
TCTCCTGTCC TGCAGTCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG AGTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTGGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AAATTGATGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTCGAGCTCT TCCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATCGAGGCC ACACACATGT ACGATAATGT GGCTGAGGCC 
TGCAGCACCA CTGAGGAGGT ATTCAACTCC CCCAGGGCTG GGTCACCCCT CTGCAATTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCGT ACCACCTTTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CAATGGAGCT GGAGGGAAAC 
GACGGGCTGT CTTTCATAAT GAGCAACTTT GAGAGTAAAC TGAACAACAA AGTACCTCCA 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GAGATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGTCATC GGGAGGGACA GAGGAAGTCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGATTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCAGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGCAATGGTT CTGACAGTGA GATGGACACA 
AGCTCTCTAG AGCGAGGCCG CAAAGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACG 
AGATGCACCC GCCTGGGGCG GTTTGCTAGT TACCGCACCA GCTTCAGTGT TGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCTTA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGGA GGAACACCAA GAAACCAAAG CCCAAACCCC GACCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCT TTAACAACAG TGGTGACTCC AGAGAAGCCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATTGAAG CCACAGGACT AAGCACAGAA 
GGCATCTACC GGGTCAGCGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGATCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCGGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAGAT CAACGACAGG GAGCAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCTAAGGA GAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AGAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGT TGATGCGGCC TGACTTCAGC AGCATGGACG CACTCACAGC CACTCGATCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCG 
ATCAGTGAGC CACCGGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCACC CACTGTCCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTCTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228474.1
CDS	1255..5754
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVIVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKSHNENWLSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDETSHIDNMENERIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDPNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDILSPVLQSQTCKSSHCGSSNSVLLELPIGVHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFEITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNRVSHNNKVNLMTSENLSICFWPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQPSPPQSPPPTPQSPMQPLLSSQLQAEHTL

Target ORF information:

RefSeq Version	XM_006228412.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228412.3

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCCGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTATGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTCAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCATTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGACTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTAGGG 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TCGATGTTAG CAGGGGCATG AACAGGAACT TCGATGACCA GCTCAAGTTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTAAT AGTCCTGACC 
AAGTGTGATG AGGGTGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CGAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTG 
GTGAGCCGCA TTGTGAAAAG TCACAATGAG AACTGGCTGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAT CTGGAAGGGA CACAGAAAGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGCCGGAG AAAGCTGTAC 
CTGGCAGCCC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTAGACCAC 
CTGAGCTGCA TTAAAGCAAA AAAGCTGTTA GAGACTAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAGACACC ATGGGATGAA ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAATGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG GGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTATTCA 
GAATTGTTTT ATGAGCTGGA GCTGGATGCT AAACCCAGTA AGGAAAAGAT GGGTGTTATC 
CAGGATGTTC TGGGTGAAGA GCAACGATTT AAAGCATTGC AGAAACTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATTCATTTT GTGTACCACC CAACAAAGGA GACATGCCCA 
AGCTGTCCAG CTTGCGTAGA TGCTAAGATT GAACATTTGA TCAGTTCTCG CTTTATCCGA 
CCATCTGATA GGAATCAGAA GAACTCTTTG TCTGACCCCA ATATTGATAG GATCAATTTG 
GTTATTTTAG GCAAAGATGG CCTTGCCCGA GAGTTAGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TTCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCG CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAAT CTACACTGGG CAGGCGGGAT AATCACTTAG TCCACCTCCC CTTGACTTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT AATACAGCAA 
GGCCAGCAGA TTGCTAGCAA ACTTCAGTGT GTCTTTCTTG ATCCTGCGTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA CGAGAAGCAG ATCAGTCAAG TTCTGAAGGG ACTCCTGGAC 
TCTAAGCGCA ACTTAAACCT GGTTAGTTCT ACTGCTAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTCATGTGT GGTGATCCTT TTAGTGCAGA TGACATTCTC 
TCTCCTGTCC TGCAGTCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG AGTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTGGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AAATTGATGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTCGAGCTCT TCCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATCGAGGCC ACACACATGT ACGATAATGT GGCTGAGGCC 
TGCAGCACCA CTGAGGAGGT ATTCAACTCC CCCAGGGCTG GGTCACCCCT CTGCAATTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCGT ACCACCTTTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CAATGGAGCT GGAGGGAAAC 
GACGGGCTGT CTTTCATAAT GAGCAACTTT GAGAGTAAAC TGAACAACAA AGTACCTCCA 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GAGATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGTCATC GGGAGGGACA GAGGAAGTCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGATTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCAGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGCAATGGTT CTGACAGTGA GATGGACACA 
AGCTCTCTAG AGCGAGGCCG CAAAGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACG 
AGATGCACCC GCCTGGGGCG GTTTGCTAGT TACCGCACCA GCTTCAGTGT TGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCTTA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGGA GGAACACCAA GAAACCAAAG CCCAAACCCC GACCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCT TTAACAACAG TGGTGACTCC AGAGAAGCCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATTGAAG CCACAGGACT AAGCACAGAA 
GGCATCTACC GGGTCAGCGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGATCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCGGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAGAT CAACGACAGG GAGCAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCTAAGGA GAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AGAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGT TGATGCGGCC TGACTTCAGC AGCATGGACG CACTCACAGC CACTCGATCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCG 
ATCAGTGAGC CACCGGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCACC CACTGTCCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTCTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The N-Terminal GTPase Domain of p190RhoGAP Proteins Is a PseudoGTPase.
Structure (London, England : 1993)26(11)1451-1461.e4(2018 11)
Stiegler AL,Boggon TJ

Symmetry breaking in spreading RAT2 fibroblasts requires the MAPK/ERK pathway scaffold RACK1 that integrates FAK, p190A-RhoGAP and ERK2 signaling.
Biochimica et biophysica acta1863(9)2189-200(2016 09)
Klímová Z,Bráborec V,Maninová M,Čáslavský J,Weber MJ,Vomastek T

Cancer-associated mutations in the protrusion-targeting region of p190RhoGAP impact tumor cell migration.
The Journal of cell biology214(7)859-73(2016 09)
Binamé F,Bidaud-Meynard A,Magnan L,Piquet L,Montibus B,Chabadel A,Saltel F,Lagrée V,Moreau V

A Point Mutation in p190A RhoGAP Affects Ciliogenesis and Leads to Glomerulocystic Kidney Defects.
PLoS genetics12(2)e1005785(2016 Feb)
Stewart K,Gaitan Y,Shafer ME,Aoudjit L,Hu D,Sharma R,Tremblay M,Ishii H,Marcotte M,Stanga D,Tang YC,Boualia SK,Nguyen AH,Takano T,Lamarche-Vane N,Vidal S,Bouchard M

Integrin α3 is required for late postnatal stability of dendrite arbors, dendritic spines and synapses, and mouse behavior.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(16)6742-52(2013 Apr)
Kerrisk ME,Greer CA,Koleske AJ

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Arhgap35 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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