alpha-PheRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alpha-PheRS
Entrez Gene ID	31740
Full Name	Phenylalanyl-tRNA synthetase, alpha-subunit
Synonyms	CG2263,Dmel\CG2263
General protein information	Preferred Names Phenylalanyl-tRNA synthetase, alpha-subunit Names CG2263 gene product from transcript CG2263-RA CG2263-PA Phenylalanyl-tRNA synthetase alpha subunit alpha-PheRS-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7D15-7D15

mRNA and Protein(s)

mRNA	Protein	Name
NM_132220.4	NP_572448.1	Phenylalanyl-tRNA synthetase, alpha-subunit

Pathways from BioSystems

Homologies

Homo sapiens (human)	FARSA	NP_004452.1
Pan troglodytes (chimpanzee)	FARSA	NP_001233393.1
Macaca mulatta (Rhesus monkey)	FARSA	XP_001110025.1
Danio rerio (zebrafish)	farsa	NP_001038760.2
Caenorhabditis elegans (roundworm)	fars-1	NP_491792.1
Saccharomyces cerevisiae (baker's yeast)	FRS2	NP_116631.1
Gallus gallus (chicken)	FARSA	NP_001258086.1
Canis lupus familiaris (dog)	FARSA	XP_533900.1
Bos taurus (cattle)	FARSA	NP_001094577.1
Rattus norvegicus (Norway rat)	Farsa	NP_001019408.1
Drosophila melanogaster (fruit fly)	CG2263	NP_572448.1
Arabidopsis thaliana (thale cress)	AT4G39280	NP_195639.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002653	XP_312272.1
Kluyveromyces lactis	KLLA0F25234g	XP_456205.1
Oryza sativa (rice)	Os10g0400800	NP_001064545.1
Schizosaccharomyces pombe (fission yeast)	frs2	NP_594078.1
Eremothecium gossypii	AGOS_AFR516W	NP_986063.1
Xenopus tropicalis (tropical clawed frog)	farsa	NP_001072827.1
Magnaporthe oryzae (rice blast fungus)	MGG_04425	XP_003713518.1
Mus musculus (house mouse)	Farsa	NP_079924.2
Neurospora crassa	NCU05095	XP_957081.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alpha-PheRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-PheRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01084	NM_132220.4 Latest version!	Drosophila melanogaster Phenylalanyl-tRNA synthetase, alpha-subunit (alpha-PheRS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alpha-PheRS ( NM_132220.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572448.1 Drosophila melanogaster Phenylalanyl-tRNA synthetase, alpha-subunit (alpha-PheRS), mRNA.

Price & Availability↑

CloneID	OFa01084
Clone ID Related Accession (Same CDS sequence)	NM_132220.4
Accession Version	NM_132220.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Phenylalanyl-tRNA synthetase, alpha-subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132220.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATCCCG ATCTCACGGA GCGCATTCTC CAGCATTTGG AGACCACCGA CAAGGCGGAC 
ACACTGGACC TGGCCGCACT CTTTGCCGAG GATCACCAGA AGATTGTGGG CTCATTGAAG 
AGCATACAGG CGCATGGCGA ACTAGTCACC GCGGAAACGG TCACCCACAA GAGTTTGGGC 
CTCACAGACG AGGGTCGCGC CGTCGTGGAG AATGGCAGTC ATGAGGCACT GGTCTACGAC 
CTAGTTCCGC CGGAGGGAAT CGCCCAGGCG GCTCTAATGG CAGCCGGTGG CGCAAATGCC 
AAAGTGGGCT TTAGCAAAGC CATGTCGCAC GGCTGGATTT TGGTGGACAA GAGCGTCACG 
CCGCCGCTGG TGCGCCGCAA GGTGGACACC ATTACGGATG TGGTGCGCAA TCAACTGCAG 
CAGGTGGCGC TGGGAAAGGG TGACCAGCTG CCTGCCAAGG AGGTGGCCGA CTTCAAGAAA 
CGCAAGCTCC TCCAGGAAAC GACCACCAAG AGCTTCGTGC TGGCACGCGG CCCTGAATTT 
GCCACCACTC TCACCAAGTT GGAGACGGAT CTCACGGTGG AAATGCTCGC AAATGGATTG 
TGGGACCAGC TCAAGTTCAA GGCCTATAAC TTTGATGCGT TGGGTGCACC ACCAACGCGT 
GGACATCTCC ATCCCTTGCT CAAAGTGCGC ACTGAGTTCC GGCAGATATT CCTCGAGATG 
GGCTTCTCAG AGATGCCTAC GAACAACTAT GTGGAATCGT CATTCTGGAA CTTCGATGCG 
CTCTATCAGC CGCAACAGCA TCCAGCTCGC GATGCCCACG ACACCTTCTT CGTGAATCAT 
CCGGCCAAAA GCCACAAGTT TCCGCAGGAC TATCTGGAGC GCGTGAAGAA GGTGCACTCT 
GTGGGTGGCT ATGGATCGAA GGGTTACGGC TACGACTGGA AGCTGGAGGA GGCGCAGAAG 
AATCTACTGC GCACGCATAC GACGGCCGTG AGTGCGAGGA TGCTGTACAA GCTGGCCAAC 
CAGGAGGGCG GCTTCAAAGC AGCCAAGTAC TTCAGCATCG ACAAGGTGTT CCGCAACGAA 
ACACTGGACG CCACACATCT GGCGGAGTTC CATCAGGTGG AGGGCGTGAT TGCAGATGTT 
GGTCTGACGC TGGGCGATCT CATTGGCACG CTGTACGAGT TCTTCAGAAA GCTGGGCATC 
ACGCAGCTTG AATTCAAGCC GGCGTACAAT CCGTACACCG AGCCCAGCAT GGAGATCTTC 
TGCTATCACC CCGGCCTGGC CAAGTGGATC GAGGTGGGCA ACTCGGGCGT CTTCCGGCCG 
GAGATGCTGC TGCCCATGGG ACTGCCGGAG AACGTGAATG TAATCGCCTG GGGTCTGTCG 
CTGGAGCGAC CCACCATGAT CAAGTACGGC ATCAACAACA TTCGCGATTT GGTCGGACCC 
AAAGTGGATC TAAAGATGGT GGAGGAGGGC CCCATCTGCC GGCTGGATCA TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572448.1
CDS	108..1604
Translation	MHPDLTERILQHLETTDKADTLDLAALFAEDHQKIVGSLKSIQAHGELVTAETVTHKSLGLTDEGRAVVENGSHEALVYDLVPPEGIAQAALMAAGGANAKVGFSKAMSHGWILVDKSVTPPLVRRKVDTITDVVRNQLQQVALGKGDQLPAKEVADFKKRKLLQETTTKSFVLARGPEFATTLTKLETDLTVEMLANGLWDQLKFKAYNFDALGAPPTRGHLHPLLKVRTEFRQIFLEMGFSEMPTNNYVESSFWNFDALYQPQQHPARDAHDTFFVNHPAKSHKFPQDYLERVKKVHSVGGYGSKGYGYDWKLEEAQKNLLRTHTTAVSARMLYKLANQEGGFKAAKYFSIDKVFRNETLDATHLAEFHQVEGVIADVGLTLGDLIGTLYEFFRKLGITQLEFKPAYNPYTEPSMEIFCYHPGLAKWIEVGNSGVFRPEMLLPMGLPENVNVIAWGLSLERPTMIKYGINNIRDLVGPKVDLKMVEEGPICRLDHA

Target ORF information:

RefSeq Version	NM_132220.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Phenylalanyl-tRNA synthetase, alpha-subunit (alpha-PheRS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132220.4

ORF Insert Sequence:

ATGCATCCCG ATCTCACGGA GCGCATTCTC CAGCATTTGG AGACCACCGA CAAGGCGGAC 
ACACTGGACC TGGCCGCACT CTTTGCCGAG GATCACCAGA AGATTGTGGG CTCATTGAAG 
AGCATACAGG CGCATGGCGA ACTAGTCACC GCGGAAACGG TCACCCACAA GAGTTTGGGC 
CTCACAGACG AGGGTCGCGC CGTCGTGGAG AATGGCAGTC ATGAGGCACT GGTCTACGAC 
CTAGTTCCGC CGGAGGGAAT CGCCCAGGCG GCTCTAATGG CAGCCGGTGG CGCAAATGCC 
AAAGTGGGCT TTAGCAAAGC CATGTCGCAC GGCTGGATTT TGGTGGACAA GAGCGTCACG 
CCGCCGCTGG TGCGCCGCAA GGTGGACACC ATTACGGATG TGGTGCGCAA TCAACTGCAG 
CAGGTGGCGC TGGGAAAGGG TGACCAGCTG CCTGCCAAGG AGGTGGCCGA CTTCAAGAAA 
CGCAAGCTCC TCCAGGAAAC GACCACCAAG AGCTTCGTGC TGGCACGCGG CCCTGAATTT 
GCCACCACTC TCACCAAGTT GGAGACGGAT CTCACGGTGG AAATGCTCGC AAATGGATTG 
TGGGACCAGC TCAAGTTCAA GGCCTATAAC TTTGATGCGT TGGGTGCACC ACCAACGCGT 
GGACATCTCC ATCCCTTGCT CAAAGTGCGC ACTGAGTTCC GGCAGATATT CCTCGAGATG 
GGCTTCTCAG AGATGCCTAC GAACAACTAT GTGGAATCGT CATTCTGGAA CTTCGATGCG 
CTCTATCAGC CGCAACAGCA TCCAGCTCGC GATGCCCACG ACACCTTCTT CGTGAATCAT 
CCGGCCAAAA GCCACAAGTT TCCGCAGGAC TATCTGGAGC GCGTGAAGAA GGTGCACTCT 
GTGGGTGGCT ATGGATCGAA GGGTTACGGC TACGACTGGA AGCTGGAGGA GGCGCAGAAG 
AATCTACTGC GCACGCATAC GACGGCCGTG AGTGCGAGGA TGCTGTACAA GCTGGCCAAC 
CAGGAGGGCG GCTTCAAAGC AGCCAAGTAC TTCAGCATCG ACAAGGTGTT CCGCAACGAA 
ACACTGGACG CCACACATCT GGCGGAGTTC CATCAGGTGG AGGGCGTGAT TGCAGATGTT 
GGTCTGACGC TGGGCGATCT CATTGGCACG CTGTACGAGT TCTTCAGAAA GCTGGGCATC 
ACGCAGCTTG AATTCAAGCC GGCGTACAAT CCGTACACCG AGCCCAGCAT GGAGATCTTC 
TGCTATCACC CCGGCCTGGC CAAGTGGATC GAGGTGGGCA ACTCGGGCGT CTTCCGGCCG 
GAGATGCTGC TGCCCATGGG ACTGCCGGAG AACGTGAATG TAATCGCCTG GGGTCTGTCG 
CTGGAGCGAC CCACCATGAT CAAGTACGGC ATCAACAACA TTCGCGATTT GGTCGGACCC 
AAAGTGGATC TAAAGATGGT GGAGGAGGGC CCCATCTGCC GGCTGGATCA TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

alpha-PheRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM