gamt cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gamt
Entrez Gene ID	796865
Full Name	guanidinoacetate N-methyltransferase
Synonyms	zgc:123136
General protein information	Preferred Names guanidinoacetate N-methyltransferase Names guanidinoacetate N-methyltransferase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105595.1	NP_001099065.1	guanidinoacetate N-methyltransferase

Pathways from BioSystems

Homologies

Homo sapiens (human)	GAMT	NP_000147.1
Macaca mulatta (Rhesus monkey)	GAMT	XP_001117362.2
Bos taurus (cattle)	GAMT	NP_001033633.1
Danio rerio (zebrafish)	gamt	NP_001099065.1
Pan troglodytes (chimpanzee)	GAMT	XP_001172605.2
Xenopus tropicalis (tropical clawed frog)	gamt	NP_988896.1
Rattus norvegicus (Norway rat)	Gamt	NP_036925.2
Mus musculus (house mouse)	Gamt	NP_034385.1

Gene Ontology
Bibliography

Related articles in PubMed

Partial depletion of yolk during zebrafish embryogenesis changes the dynamics of methionine cycle and metabolic genes.
Huang Y, Linsen SE
BMC genomics16427(2015 Jun)

Toxicological assessment of trace β-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
Yin X, Wang H, Zhang Y, Dahlgren RA, Zhang H, Shi M, Gao M, Wang X
PloS one9(7)e102731(2014)

α3Na+/K+-ATPase deficiency causes brain ventricle dilation and abrupt embryonic motility in zebrafish.
Doğanli C, Beck HC, Ribera AB, Oxvig C, Lykke-Hartmann K
The Journal of biological chemistry288(13)8862-74(2013 Mar)

Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.
Wang L, Chen D, Yang L, Huang S, Zhang Y, Zhang H
Journal of fish biology76(5)1212-9(2010 Apr)

Spatiotemporal expression of the creatine metabolism related genes agat, gamt and ct1 during zebrafish embryogenesis.
Wang L, Zhang Y, Shao M, Zhang H
The International journal of developmental biology51(3)247-53(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gamt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gamt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01178	NM_001105595.1 Latest version!	Danio rerio guanidinoacetate N-methyltransferase (gamt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gamt ( NM_001105595.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001099065.1 Danio rerio guanidinoacetate N-methyltransferase (gamt), mRNA.

Price & Availability↑

CloneID	ODa01178
Clone ID Related Accession (Same CDS sequence)	NM_001105595.1
Accession Version	NM_001105595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Danio rerio(zebrafish)
Product	guanidinoacetate N-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC153601.1. On or before Feb 21, 2008 this sequence version replaced NM_205741.1, XM_001334562.1. ##Evidence-Data-START## Transcript exon combination :: BC153601.1, AF425745.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGCCG CTCAGCCGAT CTTCTCAAAG GGCGAAAACT GCAAACAAGT GTGGCACGAC 
GCGAACGCAG ACTATAATGC GGCCGACACA CACCTGGAGA TCATGGGGAA ACCCGTCATG 
GAGCGCTGGG AAACTCCATA CATGCACTCG CTTGCAACAG TCGCGGCGTC TAAAGGTGGA 
AGGGTGCTGG AGATCGGCTT TGGGATGGCC ATAGCAGCTA CTAAAGTGGA GTCCTTCCCC 
ATCGAGGAGC ACTGGATCAT TGAGTGTAAT GACGGCGTAT TCCAGAGGCT ACAGGAATGG 
GCCAAGAGTC AGCCACATAA AGTTGTCCCG CTGAAAGGCC TGTGGGAAGA GGTCGCCCCA 
ACCCTACCTG ACAATCACTT TGACGGCATT CTCTATGACA CATACCCTTT GTCAGAGGAG 
ACCTGGCACA CACATCAGTT CAACTTTATC AAGGCTCACG CTCACAGAAT GCTGAAGCCT 
GGCGGCGTGC TCACATACTG TAACCTCACG TCCTGGGGAG AGCTGCTCAA AAACAAATAC 
GACAACATAG ACAAAATGTT CCAAGAGACA CAAGTTCCTC ACCTGCTGGA AGCTGGGTTC 
AAGAAAGAGA AGATCAGCAC CACATTGATG GACATCAGTC CTCCCAGCGA ATGCAAATAC 
TATTCCTTCA ATAAGATGAT CACACCAACT ATTATTAAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099065.1
CDS	34..738
Translation	MSAAQPIFSKGENCKQVWHDANADYNAADTHLEIMGKPVMERWETPYMHSLATVAASKGGRVLEIGFGMAIAATKVESFPIEEHWIIECNDGVFQRLQEWAKSQPHKVVPLKGLWEEVAPTLPDNHFDGILYDTYPLSEETWHTHQFNFIKAHAHRMLKPGGVLTYCNLTSWGELLKNKYDNIDKMFQETQVPHLLEAGFKKEKISTTLMDISPPSECKYYSFNKMITPTIIKE

Target ORF information:

RefSeq Version	NM_001105595.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanidinoacetate N-methyltransferase (gamt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105595.1

ORF Insert Sequence:

ATGAGCGCCG CTCAGCCGAT CTTCTCAAAG GGCGAAAACT GCAAACAAGT GTGGCACGAC 
GCGAACGCAG ACTATAATGC GGCCGACACA CACCTGGAGA TCATGGGGAA ACCCGTCATG 
GAGCGCTGGG AAACTCCATA CATGCACTCG CTTGCAACAG TCGCGGCGTC TAAAGGTGGA 
AGGGTGCTGG AGATCGGCTT TGGGATGGCC ATAGCAGCTA CTAAAGTGGA GTCCTTCCCC 
ATCGAGGAGC ACTGGATCAT TGAGTGTAAT GACGGCGTAT TCCAGAGGCT ACAGGAATGG 
GCCAAGAGTC AGCCACATAA AGTTGTCCCG CTGAAAGGCC TGTGGGAAGA GGTCGCCCCA 
ACCCTACCTG ACAATCACTT TGACGGCATT CTCTATGACA CATACCCTTT GTCAGAGGAG 
ACCTGGCACA CACATCAGTT CAACTTTATC AAGGCTCACG CTCACAGAAT GCTGAAGCCT 
GGCGGCGTGC TCACATACTG TAACCTCACG TCCTGGGGAG AGCTGCTCAA AAACAAATAC 
GACAACATAG ACAAAATGTT CCAAGAGACA CAAGTTCCTC ACCTGCTGGA AGCTGGGTTC 
AAGAAAGAGA AGATCAGCAC CACATTGATG GACATCAGTC CTCCCAGCGA ATGCAAATAC 
TATTCCTTCA ATAAGATGAT CACACCAACT ATTATTAAAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Partial depletion of yolk during zebrafish embryogenesis changes the dynamics of methionine cycle and metabolic genes.
BMC genomics16427(2015 Jun)
Huang Y,Linsen SE

Toxicological assessment of trace β-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
PloS one9(7)e102731(2014)
Yin X,Wang H,Zhang Y,Dahlgren RA,Zhang H,Shi M,Gao M,Wang X

α3Na+/K+-ATPase deficiency causes brain ventricle dilation and abrupt embryonic motility in zebrafish.
The Journal of biological chemistry288(13)8862-74(2013 Mar)
Doğanli C,Beck HC,Ribera AB,Oxvig C,Lykke-Hartmann K

Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.
Journal of fish biology76(5)1212-9(2010 Apr)
Wang L,Chen D,Yang L,Huang S,Zhang Y,Zhang H

Spatiotemporal expression of the creatine metabolism related genes agat, gamt and ct1 during zebrafish embryogenesis.
The International journal of developmental biology51(3)247-53(2007)
Wang L,Zhang Y,Shao M,Zhang H