nphs1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nphs1
Entrez Gene ID	692352
Full Name	nephrosis 1, congenital, Finnish type (nephrin)
Synonyms	nphs1l
General protein information	Preferred Names nephrosis 1, congenital, Finnish type (nephrin) Names nephrin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351014.1	XP_017206503.1	nephrin isoform X1
XM_017351014.2	XP_017206503.1	nephrin isoform X1
NM_001040687.1	NP_001035777.1	nephrin
XM_021481465.1	XP_021337140.1	nephrin isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPHS1	XP_524228.3
Homo sapiens (human)	NPHS1	NP_004637.1
Rattus norvegicus (Norway rat)	Nphs1	NP_072150.1
Mus musculus (house mouse)	Nphs1	NP_062332.2
Drosophila melanogaster (fruit fly)	sns	NP_788286.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010742	XP_311455.4
Macaca mulatta (Rhesus monkey)	NPHS1	XP_001112314.1
Canis lupus familiaris (dog)	NPHS1	XP_541685.3
Bos taurus (cattle)	NPHS1	NP_001179441.1
Danio rerio (zebrafish)	nphs1	NP_001035777.1
Xenopus tropicalis (tropical clawed frog)	nphs1	XP_002940674.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic control of cellular morphogenesis in Müller glia.
Charlton-Perkins M, Almeida AD, MacDonald RB, Harris WA
Glia67(7)1401-1411(2019 07)

Wnt signaling mediates new nephron formation during zebrafish kidney regeneration.
Kamei CN, Gallegos TF, Liu Y, Hukriede N, Drummond IA
Development (Cambridge, England)146(8)(2019 04)

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development.
Tian J, Shao J, Liu C, Hou HY, Chou CW, Shboul M, Li GQ, El-Khateeb M, Samarah OQ, Kou Y, Chen YH, Chen MJ, Lyu Z, Chen WL, Chen YF, Sun YH, Liu YW
Cellular and molecular life sciences : CMLS76(1)163-178(2019 Jan)

RNA sequencing of isolated cell populations expressing human APOL1 G2 risk variant reveals molecular correlates of sickle cell nephropathy in zebrafish podocytes.
Bundy JL, Anderson BR, Francescatto L, Garrett ME, Soldano KL, Telen MJ, Davis EE, Ashley-Koch AE
PloS one14(6)e0217042(2019)

The transcription factor Dach1 is essential for podocyte function.
Endlich N, Kliewe F, Kindt F, Schmidt K, Kotb AM, Artelt N, Lindenmeyer MT, Cohen CD, Döring F, Kuss AW, Amann K, Moeller MJ, Kabgani N, Blumenthal A, Endlich K
Journal of cellular and molecular medicine22(5)2656-2669(2018 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02033	NM_001040687.1 Latest version!	Danio rerio NPHS1 adhesion molecule, nephrin (nphs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa55129	XM_021481465.1 Latest version!	Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa55129	XM_017351014.2 Latest version!	Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa55129	XM_017351014.1	Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nphs1 ( NM_001040687.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001035777.1 Danio rerio NPHS1 adhesion molecule, nephrin (nphs1), mRNA.

Price & Availability↑

CloneID	ODa02033
Clone ID Related Accession (Same CDS sequence)	NM_001040687.1
Accession Version	NM_001040687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	nephrin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ466904.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ466904.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATTCCT GGCTTTCTCT AGTCTTCTCG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCG 
GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT 
AAAGACGGGC TGCTCCTGAG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG 
ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC 
GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT 
ACAGTCTGGC TCAATGTGCA GATCCCTTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG 
GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT 
CCTGCAGAAA TCACAATATT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA 
ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA 
AGCTCGGACG ACACGCGACG ACTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT 
CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA 
TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG 
GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG 
GATGTAGACT TTGTGTCCCG TCGAGCCAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG 
GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC 
CTAACCCGCA CTCTTACTGT CCCCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT 
GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC 
GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC 
GAGGGGGATA ATGGTGGGAT GATGACAGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG 
GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT 
CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA 
CCAAATGTCT TTTTACACTC AGGCTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA 
AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG 
CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCACCCTCCA TCCCAGTGAC 
AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCGCCCAA 
ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG 
CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA 
AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG 
GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ACCCCATCAC 
TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT 
TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG CTGAGCAGCC TGATGAAGTC 
CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA 
ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT 
ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC 
ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA 
TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCTGTGTTTG TGAACATAGG AGACACTGCA 
GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG 
ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG 
TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC 
GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT 
CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT 
GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA GAATGGGTTC 
CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCAACAGG CACCATCCAC 
ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCCTTGG ACTATGCCAT ATTCACCTGC 
ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC 
CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG 
TGGATGCCCG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA 
GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC 
ACAGGCCTTA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGG 
GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGACAGTCA CCACTGCTGA TGCGAAGGAA 
GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCAGCTC TTGCAGGTGG TGGCCTTCCT 
GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC 
TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGAACAC CAGCAGCATT 
TCACAAGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG 
AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCGGAG 
CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA 
ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTGCGATGGC 
AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC 
CTGCAGGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC 
ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT 
GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG 
CTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035777.1
CDS	13..3741
Translation	MDSWLSLVFSSGCFLLLVDFKGVCGQQAFKTEPRNVTVRAGATALLKCEVLRASGAVQWVKDGLLLRPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESEEPWVEGEQYTVTCIAPDAKPPAEITIFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPRALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVCMSYGGNPLATLHWTKNGEVLSTSWDVDFVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVPFEPAEVTLLGSFEAVEGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLTLHPSDNLATYRCDSSNKAKKVLSAQTKFRVLFPAVSVKIVAKQKELRRGQTLDLDCLAGSSNPTANISWSLGPVILKGVDQAPKRAEFGGMSRTSKLSLTLEPHHWRKRITCQAFSNVLSEGVNNFYSLDVLFPPEFSAEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSASYMYVDVFPPTATVYTVTGLNPSTMYNFSVNALNSIGESSYADNNKILTVTTADAKEADGGVSTADQDSALAGGGLPVYVVVILVVGGVLFLLNPFICFLLARWKRRRSLKGNTSSISQETKSEEQRSTASSSTRYASRELINASAQRTLLVDSSSEPDSSVYESYGRQENPHYYYPTEAYSPALYAHPEGPEDCDGRHGTSPFTHVYEEVRGMYEDLQACSPLPPAAHIHPQAPFSTYYEGRAYSKADVDVTIGAYDSMVYRRRRDTDLPFELRGELV

Target ORF information:

RefSeq Version	NM_001040687.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NPHS1 adhesion molecule, nephrin (nphs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040687.1

ORF Insert Sequence:

ATGGATTCCT GGCTTTCTCT AGTCTTCTCG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCG 
GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT 
AAAGACGGGC TGCTCCTGAG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG 
ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC 
GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT 
ACAGTCTGGC TCAATGTGCA GATCCCTTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG 
GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT 
CCTGCAGAAA TCACAATATT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA 
ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA 
AGCTCGGACG ACACGCGACG ACTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT 
CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA 
TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG 
GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG 
GATGTAGACT TTGTGTCCCG TCGAGCCAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG 
GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC 
CTAACCCGCA CTCTTACTGT CCCCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT 
GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC 
GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC 
GAGGGGGATA ATGGTGGGAT GATGACAGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG 
GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT 
CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA 
CCAAATGTCT TTTTACACTC AGGCTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA 
AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG 
CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCACCCTCCA TCCCAGTGAC 
AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCGCCCAA 
ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG 
CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA 
AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG 
GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ACCCCATCAC 
TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT 
TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG CTGAGCAGCC TGATGAAGTC 
CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA 
ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT 
ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC 
ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA 
TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCTGTGTTTG TGAACATAGG AGACACTGCA 
GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG 
ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG 
TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC 
GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT 
CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT 
GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA GAATGGGTTC 
CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCAACAGG CACCATCCAC 
ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCCTTGG ACTATGCCAT ATTCACCTGC 
ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC 
CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG 
TGGATGCCCG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA 
GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC 
ACAGGCCTTA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGG 
GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGACAGTCA CCACTGCTGA TGCGAAGGAA 
GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCAGCTC TTGCAGGTGG TGGCCTTCCT 
GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC 
TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGAACAC CAGCAGCATT 
TCACAAGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG 
AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCGGAG 
CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA 
ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTGCGATGGC 
AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC 
CTGCAGGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC 
ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT 
GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG 
CTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphs1 ( XM_017351014.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206503.1 Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55129
Clone ID Related Accession (Same CDS sequence)	XM_017351014.1 , XM_017351014.2 , XM_021481465.1
Accession Version	XM_017351014.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nephrin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA 
GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT 
AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG 
ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC 
GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT 
ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG 
GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT 
CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA 
ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA 
AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT 
CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA 
TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG 
GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG 
GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG 
GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC 
CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT 
GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC 
GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC 
GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG 
GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT 
CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA 
CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA 
AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG 
CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC 
AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA 
ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG 
CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA 
AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG 
GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC 
TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT 
TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC 
CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA 
ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT 
ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC 
ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA 
TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA 
GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG 
ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG 
TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC 
GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT 
CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT 
GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC 
CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC 
ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC 
ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC 
CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG 
TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA 
GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC 
ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA 
GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA 
GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT 
GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC 
TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT 
TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG 
AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG 
CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA 
ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC 
AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC 
CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC 
ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT 
GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG 
CTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206503.1
CDS	290..4018
Translation	MDSWLSLVFLSGCFLLLVDFKGVCGQQAFKTEPRNVTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESEEPWVEGEQYTVTCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPRALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVCMSYGGNPLATLHWTKNGEVLSTSWDVDFVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLLGSFEAVEGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKFRVLFPAVSVKIVAKQKELRRGQTLDLDCLAGSSNPTANISWSLGPVILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEGVNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSASYMYVDVFPPTATVYTVTGLNPSTMYNFSVNALNSIGESSYADNNKILMVTTADAKEADGGVSTADQDSALAGGGLPVYVVVILVVGGVLFLLNPFICFLLARWKRRRSLKGDTSSISQETKSEEQRSTASSSTRYASRELINASAQRTLLVDSSSEPDSSVYESYGRQENPHYYYPTEAYSPALYAHPEGPEDYDGRHGTSPFTHVYEEVRGMYEDLQACSPLPPAAHIHPQAPFSTYYEGRAYSKADVDVTIGAYDSMVYRRRRDTDLPFELRGELV

Target ORF information:

RefSeq Version	XM_017351014.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351014.1

ORF Insert Sequence:

ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA 
GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT 
AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG 
ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC 
GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT 
ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG 
GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT 
CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA 
ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA 
AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT 
CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA 
TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG 
GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG 
GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG 
GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC 
CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT 
GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC 
GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC 
GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG 
GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT 
CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA 
CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA 
AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG 
CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC 
AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA 
ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG 
CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA 
AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG 
GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC 
TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT 
TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC 
CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA 
ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT 
ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC 
ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA 
TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA 
GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG 
ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG 
TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC 
GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT 
CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT 
GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC 
CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC 
ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC 
ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC 
CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG 
TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA 
GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC 
ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA 
GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA 
GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT 
GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC 
TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT 
TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG 
AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG 
CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA 
ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC 
AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC 
CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC 
ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT 
GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG 
CTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphs1 ( XM_017351014.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206503.1 Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X2, mRNA.

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CloneID	ODa55129
Clone ID Related Accession (Same CDS sequence)	XM_017351014.1 , XM_017351014.2 , XM_021481465.1
Accession Version	XM_017351014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nephrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA 
GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT 
AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG 
ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC 
GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT 
ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG 
GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT 
CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA 
ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA 
AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT 
CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA 
TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG 
GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG 
GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG 
GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC 
CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT 
GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC 
GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC 
GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG 
GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT 
CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA 
CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA 
AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG 
CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC 
AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA 
ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG 
CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA 
AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG 
GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC 
TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT 
TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC 
CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA 
ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT 
ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC 
ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA 
TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA 
GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG 
ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG 
TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC 
GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT 
CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT 
GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC 
CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC 
ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC 
ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC 
CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG 
TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA 
GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC 
ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA 
GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA 
GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT 
GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC 
TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT 
TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG 
AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG 
CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA 
ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC 
AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC 
CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC 
ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT 
GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG 
CTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206503.1
CDS	605..4333
Translation	MDSWLSLVFLSGCFLLLVDFKGVCGQQAFKTEPRNVTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESEEPWVEGEQYTVTCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPRALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVCMSYGGNPLATLHWTKNGEVLSTSWDVDFVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLLGSFEAVEGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKFRVLFPAVSVKIVAKQKELRRGQTLDLDCLAGSSNPTANISWSLGPVILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEGVNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSASYMYVDVFPPTATVYTVTGLNPSTMYNFSVNALNSIGESSYADNNKILMVTTADAKEADGGVSTADQDSALAGGGLPVYVVVILVVGGVLFLLNPFICFLLARWKRRRSLKGDTSSISQETKSEEQRSTASSSTRYASRELINASAQRTLLVDSSSEPDSSVYESYGRQENPHYYYPTEAYSPALYAHPEGPEDYDGRHGTSPFTHVYEEVRGMYEDLQACSPLPPAAHIHPQAPFSTYYEGRAYSKADVDVTIGAYDSMVYRRRRDTDLPFELRGELV

Target ORF information:

RefSeq Version	XM_017351014.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351014.2

ORF Insert Sequence:

ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA 
GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT 
AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG 
ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC 
GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT 
ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG 
GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT 
CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA 
ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA 
AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT 
CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA 
TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG 
GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG 
GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG 
GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC 
CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT 
GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC 
GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC 
GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG 
GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT 
CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA 
CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA 
AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG 
CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC 
AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA 
ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG 
CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA 
AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG 
GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC 
TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT 
TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC 
CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA 
ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT 
ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC 
ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA 
TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA 
GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG 
ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG 
TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC 
GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT 
CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT 
GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC 
CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC 
ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC 
ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC 
CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG 
TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA 
GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC 
ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA 
GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA 
GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT 
GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC 
TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT 
TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG 
AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG 
CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA 
ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC 
AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC 
CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC 
ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT 
GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG 
CTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphs1 ( XM_021481465.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337140.1 Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA.

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CloneID	ODa55129
Clone ID Related Accession (Same CDS sequence)	XM_017351014.1 , XM_017351014.2 , XM_021481465.1
Accession Version	XM_021481465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nephrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA 
GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT 
AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG 
ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC 
GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT 
ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG 
GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT 
CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA 
ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA 
AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT 
CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA 
TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG 
GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG 
GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG 
GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC 
CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT 
GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC 
GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC 
GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG 
GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT 
CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA 
CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA 
AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG 
CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC 
AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA 
ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG 
CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA 
AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG 
GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC 
TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT 
TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC 
CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA 
ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT 
ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC 
ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA 
TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA 
GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG 
ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG 
TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC 
GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT 
CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT 
GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC 
CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC 
ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC 
ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC 
CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG 
TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA 
GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC 
ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA 
GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA 
GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT 
GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC 
TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT 
TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG 
AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG 
CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA 
ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC 
AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC 
CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC 
ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT 
GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG 
CTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337140.1
CDS	489..4217
Translation	MDSWLSLVFLSGCFLLLVDFKGVCGQQAFKTEPRNVTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESEEPWVEGEQYTVTCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPRALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVCMSYGGNPLATLHWTKNGEVLSTSWDVDFVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLLGSFEAVEGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKFRVLFPAVSVKIVAKQKELRRGQTLDLDCLAGSSNPTANISWSLGPVILKGVDQAPKRAEFGGMSRTSKLSLTLESHHWRKRITCQAFSNVLSEGVNNFYSLDVLFPPEFSDEQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSASYMYVDVFPPTATVYTVTGLNPSTMYNFSVNALNSIGESSYADNNKILMVTTADAKEADGGVSTADQDSALAGGGLPVYVVVILVVGGVLFLLNPFICFLLARWKRRRSLKGDTSSISQETKSEEQRSTASSSTRYASRELINASAQRTLLVDSSSEPDSSVYESYGRQENPHYYYPTEAYSPALYAHPEGPEDYDGRHGTSPFTHVYEEVRGMYEDLQACSPLPPAAHIHPQAPFSTYYEGRAYSKADVDVTIGAYDSMVYRRRRDTDLPFELRGELV

Target ORF information:

RefSeq Version	XM_021481465.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481465.1

ORF Insert Sequence:

ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA 
GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT 
AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG 
ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC 
GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT 
ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG 
GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT 
CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA 
ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA 
AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT 
CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA 
TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG 
GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG 
GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG 
GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC 
CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT 
GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC 
GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC 
GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG 
GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT 
CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA 
CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA 
AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG 
CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC 
AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA 
ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG 
CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA 
AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG 
GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC 
TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT 
TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC 
CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA 
ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT 
ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC 
ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA 
TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA 
GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG 
ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG 
TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC 
GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT 
CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT 
GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC 
CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC 
ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC 
ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC 
CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG 
TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA 
GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC 
ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA 
GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA 
GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT 
GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC 
TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT 
TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG 
AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG 
CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA 
ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC 
AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC 
CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC 
ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT 
GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG 
CTGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic control of cellular morphogenesis in Müller glia.
Glia67(7)1401-1411(2019 07)
Charlton-Perkins M,Almeida AD,MacDonald RB,Harris WA

Wnt signaling mediates new nephron formation during zebrafish kidney regeneration.
Development (Cambridge, England)146(8)(2019 04)
Kamei CN,Gallegos TF,Liu Y,Hukriede N,Drummond IA

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development.
Cellular and molecular life sciences : CMLS76(1)163-178(2019 Jan)
Tian J,Shao J,Liu C,Hou HY,Chou CW,Shboul M,Li GQ,El-Khateeb M,Samarah OQ,Kou Y,Chen YH,Chen MJ,Lyu Z,Chen WL,Chen YF,Sun YH,Liu YW

RNA sequencing of isolated cell populations expressing human APOL1 G2 risk variant reveals molecular correlates of sickle cell nephropathy in zebrafish podocytes.
PloS one14(6)e0217042(2019)
Bundy JL,Anderson BR,Francescatto L,Garrett ME,Soldano KL,Telen MJ,Davis EE,Ashley-Koch AE

The transcription factor Dach1 is essential for podocyte function.
Journal of cellular and molecular medicine22(5)2656-2669(2018 05)
Endlich N,Kliewe F,Kindt F,Schmidt K,Kotb AM,Artelt N,Lindenmeyer MT,Cohen CD,Döring F,Kuss AW,Amann K,Moeller MJ,Kabgani N,Blumenthal A,Endlich K

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nphs1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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