NPHS1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPHS1
Entrez Gene ID	484571
Full Name	NPHS1, nephrin
General protein information	Preferred Names NPHS1, nephrin Names nephrin nephrosis 1, congenital, Finnish type (nephrin)
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_541685.5	XP_541685.3	nephrin isoform X1
XM_022403540.1	XP_022259248.1	nephrin isoform X4
XM_005616692.3	XP_005616749.1	nephrin isoform X2
XM_022403537.1	XP_022259245.1	nephrin isoform X3
XM_541685.6	XP_541685.3	nephrin isoform X1
XM_005616691.2	XP_005616748.1	nephrin isoform X2
XM_005616692.2	XP_005616749.1	nephrin isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPHS1	XP_524228.3
Homo sapiens (human)	NPHS1	NP_004637.1
Rattus norvegicus (Norway rat)	Nphs1	NP_072150.1
Mus musculus (house mouse)	Nphs1	NP_062332.2
Drosophila melanogaster (fruit fly)	sns	NP_788286.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010742	XP_311455.4
Macaca mulatta (Rhesus monkey)	NPHS1	XP_001112314.1
Canis lupus familiaris (dog)	NPHS1	XP_541685.3
Bos taurus (cattle)	NPHS1	NP_001179441.1
Danio rerio (zebrafish)	nphs1	NP_001035777.1
Xenopus tropicalis (tropical clawed frog)	nphs1	XP_002940674.2

Gene Ontology

The following NPHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe02321	XM_541685.6 Latest version!	Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCe02322	XM_005616691.2 Latest version!	Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCe02323	XM_005616692.3 Latest version!	Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCe286452	XM_022403537.1 Latest version!	Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe286453	XM_022403540.1 Latest version!	Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe02321	XM_541685.5	Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCe02323	XM_005616692.2	Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPHS1 ( XM_541685.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_541685.3 Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe02321
Clone ID Related Accession (Same CDS sequence)	XM_541685.5 , XM_541685.6
Accession Version	XM_541685.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	nephrin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_541685.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC 
GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC 
ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG 
GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC 
GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT 
CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG 
TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG 
GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT 
CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG 
AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC 
GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT 
CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT 
ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG 
GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA 
CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA 
GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC 
AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC 
GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC 
AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC 
AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC 
TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA 
AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG 
GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC 
ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA 
CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG 
GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC 
CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT 
GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG 
CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG 
GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC 
TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC 
CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG 
GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT 
GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGGGCC CCTCTGACCC CTCCTGGCCT 
GAGGAGACAT ATGAGGATAC AAGAAGAATC TATGATCGGG TAGCAGGAAA CTTGGACCCT 
GTGGAAACTG ATTCTCTACC CTTTGAGCTG AGGGGACATC TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_541685.3
CDS	2..3889
Translation	MEREENREIEGDEWMKRDNKKSQTHGERDPAKDRTRDRRQAVGTVGATARGPRMALRTPSRAPLLLMGLLTTGLAQLPLSASAPRGFWALPENLTVVEGATAELQCGVHAPGSVVQWAKDGLLLGPDPRIPRFPRYRLEGDPSRGEFHLHIEACDLSDDAEYECQVGRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFFQSGQTVSDISANVNAGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQSLELLCVARGGNPPATLQWLKNGQPMSTAWGTEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKAGQLSASTQLVVQFPPTNVTILANATALRPGDALNLTCISVSSNPPVNLSWDKEGERLESVATPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAETLVRLDVHYAPTIRALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLGEEEEDQSLDDMEKISKGSTGRLRIHHAKLAQAGAYQCIVDNGVAPPARGLVRLVVRFAPQVEHPTPLNKVAAAGDSTSSATLHCRARGVPNIVFTWAKNGVPLDLEDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTNIQLVSISRPDPPTGLKVVSMTPYSVGLEWKPGFDGGLPQKFQIRYEALGTPGFLYMDVLPPDATTFTLTGLQPSTRYRVWLLASNALGDSGLTDKGSQIPITTPGLDEPSGEPDYQLPTEQPVGPSGLPLLPVLFAVGGLLLLSNISCVGGFLWQRRLRRLAEGISEKTETGSEEDRVRNEYEDSRWTGDQDTRSSTVSTTDVEPYYHSMKNFSPQLPPTLEEMSYLRGFTHIEEENMAFPGHLYDEVERLYAPPGAWGPIYDEVPMGPSDPSWPEETYEDTRRIYDRVAGNLDPVETDSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_541685.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_541685.5

ORF Insert Sequence:

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC 
GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC 
ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG 
GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC 
GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT 
CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG 
TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG 
GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT 
CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG 
AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC 
GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT 
CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT 
ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG 
GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA 
CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA 
GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC 
AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC 
GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC 
AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC 
AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC 
TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA 
AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG 
GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC 
ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA 
CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG 
GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC 
CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT 
GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG 
CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG 
GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC 
TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC 
CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG 
GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT 
GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGGGCC CCTCTGACCC CTCCTGGCCT 
GAGGAGACAT ATGAGGATAC AAGAAGAATC TATGATCGGG TAGCAGGAAA CTTGGACCCT 
GTGGAAACTG ATTCTCTACC CTTTGAGCTG AGGGGACATC TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHS1 ( XM_541685.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_541685.3 Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe02321
Clone ID Related Accession (Same CDS sequence)	XM_541685.5 , XM_541685.6
Accession Version	XM_541685.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	nephrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_541685.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC 
GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC 
ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG 
GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC 
GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT 
CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG 
TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG 
GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT 
CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG 
AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC 
GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT 
CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT 
ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG 
GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA 
CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA 
GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC 
AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC 
GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC 
AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC 
AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC 
TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA 
AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG 
GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC 
ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA 
CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG 
GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC 
CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT 
GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG 
CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG 
GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC 
TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC 
CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG 
GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT 
GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGGGCC CCTCTGACCC CTCCTGGCCT 
GAGGAGACAT ATGAGGATAC AAGAAGAATC TATGATCGGG TAGCAGGAAA CTTGGACCCT 
GTGGAAACTG ATTCTCTACC CTTTGAGCTG AGGGGACATC TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_541685.3
CDS	294..4181
Translation	MEREENREIEGDEWMKRDNKKSQTHGERDPAKDRTRDRRQAVGTVGATARGPRMALRTPSRAPLLLMGLLTTGLAQLPLSASAPRGFWALPENLTVVEGATAELQCGVHAPGSVVQWAKDGLLLGPDPRIPRFPRYRLEGDPSRGEFHLHIEACDLSDDAEYECQVGRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFFQSGQTVSDISANVNAGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQSLELLCVARGGNPPATLQWLKNGQPMSTAWGTEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKAGQLSASTQLVVQFPPTNVTILANATALRPGDALNLTCISVSSNPPVNLSWDKEGERLESVATPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAETLVRLDVHYAPTIRALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLGEEEEDQSLDDMEKISKGSTGRLRIHHAKLAQAGAYQCIVDNGVAPPARGLVRLVVRFAPQVEHPTPLNKVAAAGDSTSSATLHCRARGVPNIVFTWAKNGVPLDLEDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTNIQLVSISRPDPPTGLKVVSMTPYSVGLEWKPGFDGGLPQKFQIRYEALGTPGFLYMDVLPPDATTFTLTGLQPSTRYRVWLLASNALGDSGLTDKGSQIPITTPGLDEPSGEPDYQLPTEQPVGPSGLPLLPVLFAVGGLLLLSNISCVGGFLWQRRLRRLAEGISEKTETGSEEDRVRNEYEDSRWTGDQDTRSSTVSTTDVEPYYHSMKNFSPQLPPTLEEMSYLRGFTHIEEENMAFPGHLYDEVERLYAPPGAWGPIYDEVPMGPSDPSWPEETYEDTRRIYDRVAGNLDPVETDSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_541685.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_541685.6

ORF Insert Sequence:

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC 
GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC 
ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG 
GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC 
GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT 
CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG 
TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG 
GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT 
CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG 
AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC 
GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT 
CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT 
ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG 
GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA 
CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA 
GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC 
AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC 
GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC 
AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC 
AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC 
TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA 
AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG 
GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC 
ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA 
CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG 
GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC 
CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT 
GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG 
CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG 
GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC 
TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC 
CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG 
GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT 
GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGGGCC CCTCTGACCC CTCCTGGCCT 
GAGGAGACAT ATGAGGATAC AAGAAGAATC TATGATCGGG TAGCAGGAAA CTTGGACCCT 
GTGGAAACTG ATTCTCTACC CTTTGAGCTG AGGGGACATC TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHS1 ( XM_005616691.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005616748.1 Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X2, mRNA.

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CloneID	OCe02322
Clone ID Related Accession (Same CDS sequence)	XM_005616691.2
Accession Version	XM_005616691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	nephrin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC 
GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC 
ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG 
GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC 
GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT 
CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG 
TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG 
GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT 
CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG 
AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC 
GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT 
CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT 
ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG 
GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA 
CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA 
GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC 
AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC 
GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC 
AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC 
AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC 
TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA 
AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG 
GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC 
ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA 
CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG 
GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC 
CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT 
GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG 
CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG 
GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC 
TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC 
CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG 
GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT 
GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGACAT ATGAGGATAC AAGAAGAATC 
TATGATCGGG TAGCAGGAAA CTTGGACCCT GTGGAAACTG ATTCTCTACC CTTTGAGCTG 
AGGGGACATC TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616748.1
CDS	2..3859
Translation	MEREENREIEGDEWMKRDNKKSQTHGERDPAKDRTRDRRQAVGTVGATARGPRMALRTPSRAPLLLMGLLTTGLAQLPLSASAPRGFWALPENLTVVEGATAELQCGVHAPGSVVQWAKDGLLLGPDPRIPRFPRYRLEGDPSRGEFHLHIEACDLSDDAEYECQVGRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFFQSGQTVSDISANVNAGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQSLELLCVARGGNPPATLQWLKNGQPMSTAWGTEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKAGQLSASTQLVVQFPPTNVTILANATALRPGDALNLTCISVSSNPPVNLSWDKEGERLESVATPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAETLVRLDVHYAPTIRALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLGEEEEDQSLDDMEKISKGSTGRLRIHHAKLAQAGAYQCIVDNGVAPPARGLVRLVVRFAPQVEHPTPLNKVAAAGDSTSSATLHCRARGVPNIVFTWAKNGVPLDLEDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTNIQLVSISRPDPPTGLKVVSMTPYSVGLEWKPGFDGGLPQKFQIRYEALGTPGFLYMDVLPPDATTFTLTGLQPSTRYRVWLLASNALGDSGLTDKGSQIPITTPGLDEPSGEPDYQLPTEQPVGPSGLPLLPVLFAVGGLLLLSNISCVGGFLWQRRLRRLAEGISEKTETGSEEDRVRNEYEDSRWTGDQDTRSSTVSTTDVEPYYHSMKNFSPQLPPTLEEMSYLRGFTHIEEENMAFPGHLYDEVERLYAPPGAWGPIYDEVPMTYEDTRRIYDRVAGNLDPVETDSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_005616691.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005616691.2

ORF Insert Sequence:

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC 
GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC 
ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG 
GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC 
GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT 
CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG 
TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG 
GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT 
CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG 
AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC 
GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT 
CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT 
ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG 
GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA 
CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA 
GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC 
AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC 
GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC 
AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC 
AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC 
TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA 
AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG 
GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC 
ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA 
CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG 
GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC 
CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT 
GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG 
CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG 
GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC 
TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC 
CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG 
GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT 
GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGACAT ATGAGGATAC AAGAAGAATC 
TATGATCGGG TAGCAGGAAA CTTGGACCCT GTGGAAACTG ATTCTCTACC CTTTGAGCTG 
AGGGGACATC TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHS1 ( XM_005616692.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005616749.1 Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X2, mRNA.

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CloneID	OCe02323
Clone ID Related Accession (Same CDS sequence)	XM_005616692.3 , XM_005616692.2
Accession Version	XM_005616692.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	nephrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG TTCCCCCAAC AAACGTGACG ATCTTGGCCA ATGCTACGGC ACTGCGCCCA 
GGGGATGCAT TAAACTTGAC TTGCATCAGT GTTAGCAGCA ACCCGCCTGT CAACTTGTCG 
TGGGACAAGG AGGGGGAGAG GCTGGAAAGT GTGGCCACAC CGCCCCGTAG TGCCCCATTC 
AAAGGCTCTG CGGCAGCCAG GAGCGTCCTT CTGAGAATGT CATCCCGAGA CCACGGCCAC 
CGAGTGACCT GCCAAGCCCA CAGTCCTGAG CTACGGGAAA CCATGAGCGC TTTCTACCGC 
CTCAACGTGC TGTATCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACCGCGGTG 
GAGCAGGGTG AGGCACTGCT GCCGGTGTCT GTGTCTGCCA ACCCTGCCCC GGAGGCCTTC 
AACTGGACTT TCCGTGGCTA CCGTCTCAGC CCAGCTGGCG GCCCTCGGCA TCGCATCTTG 
TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGTGCTG ACGATGGCCT CTATCAGCTG 
CATTGCCAGA ACTCAGAGGG CACCGCCGAA ACTCTAGTGC GGCTGGATGT ACACTATGCT 
CCCACTATCC GAGCTCTCCA GGATCCTACT GAGGTGAATA TTGGGGGTTC TGTGGACATA 
GTCTGCACTG TTGATGCCAA TCCTATCCTT CCAGAGATGT TCAGCTGGGA GAGGCTGGGA 
GAAGAGGAGG AGGACCAGAG CCTGGATGAC ATGGAGAAGA TATCAAAAGG ATCGACAGGG 
CGTCTTCGAA TTCACCATGC CAAACTGGCC CAGGCTGGTG CTTACCAGTG CATCGTGGAT 
AATGGAGTGG CACCTCCAGC CCGAGGGTTG GTCCGACTTG TTGTCAGATT CGCCCCCCAG 
GTGGAGCACC CCACTCCCCT AAACAAGGTG GCTGCAGCTG GAGACAGTAC AAGTTCTGCC 
ACTCTCCACT GTCGTGCCCG AGGCGTCCCC AACATCGTCT TCACTTGGGC CAAAAATGGA 
GTCCCTCTGG ATCTCGAGGA TCCCAGGTAC ACAGAGCACA CATACCACCA GGGTGGGGTC 
CACAGCAGTC TCCTGACCAT TGCCAACGTG TCTGCAGCCC AGGATTATGC CCTCTTCACG 
TGCACAGCCA CCAACCCCCT TGGCTCAGAC CACACCAACA TTCAACTCGT CAGCATTAGC 
CGCCCGGATC CCCCAACAGG ATTAAAGGTT GTAAGCATGA CCCCATATTC GGTGGGGTTG 
GAATGGAAGC CTGGCTTTGA TGGGGGTTTG CCACAGAAGT TCCAAATCAG ATATGAGGCA 
CTTGGGACTC CAGGGTTCCT CTACATGGAC GTCCTACCGC CTGATGCCAC CACCTTCACA 
CTAACTGGGC TACAGCCTTC TACACGATAC AGGGTCTGGC TGCTAGCCAG CAATGCCCTG 
GGGGACAGCG GACTAACTGA CAAGGGGTCC CAGATCCCTA TTACCACCCC AGGTCTAGAC 
GAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGTAGG ACCCTCAGGG 
CTGCCTCTAC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTT TGCTGCTTTC TAATATCTCC 
TGTGTTGGAG GATTCCTCTG GCAGCGGAGA CTGAGGCGTC TTGCTGAAGG CATCTCGGAG 
AAGACAGAGA CAGGGTCGGA GGAGGACCGA GTCAGGAATG AATATGAGGA CAGCCGGTGG 
ACTGGAGACC AGGACACTCG AAGCTCCACA GTTAGCACAA CAGATGTGGA ACCATATTAC 
CACTCCATGA AGAACTTCAG CCCCCAGCTG CCACCAACAC TGGAGGAGAT GTCTTATCTC 
CGAGGTTTCA CACATATTGA AGAGGAGAAT ATGGCTTTTC CTGGACACCT GTATGATGAA 
GTGGAAAGGC TATATGCCCC GCCTGGGGCC TGGGGACCCA TCTATGATGA AGTACCAATG 
GGCCCCTCTG ACCCCTCCTG GCCTGAGGAG ACATATGAGG ATACAAGAAG AATCTATGAT 
CGGGTAGCAG GAAACTTGGA CCCTGTGGAA ACTGATTCTC TACCCTTTGA GCTGAGGGGA 
CATCTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616749.1
CDS	295..4146
Translation	MEREENREIEGDEWMKRDNKKSQTHGERDPAKDRTRDRRQAVGTVGATARGPRMALRTPSRAPLLLMGLLTTGLAQLPLSASAPRGFWALPENLTVVEGATAELQCGVHAPGSVVQWAKDGLLLGPDPRIPRFPRYRLEGDPSRGEFHLHIEACDLSDDAEYECQVGRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFFQSGQTVSDISANVNAGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQSLELLCVARGGNPPATLQWLKNGQPMSTAWGTEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKAVPPTNVTILANATALRPGDALNLTCISVSSNPPVNLSWDKEGERLESVATPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAETLVRLDVHYAPTIRALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLGEEEEDQSLDDMEKISKGSTGRLRIHHAKLAQAGAYQCIVDNGVAPPARGLVRLVVRFAPQVEHPTPLNKVAAAGDSTSSATLHCRARGVPNIVFTWAKNGVPLDLEDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTNIQLVSISRPDPPTGLKVVSMTPYSVGLEWKPGFDGGLPQKFQIRYEALGTPGFLYMDVLPPDATTFTLTGLQPSTRYRVWLLASNALGDSGLTDKGSQIPITTPGLDEPSGEPDYQLPTEQPVGPSGLPLLPVLFAVGGLLLLSNISCVGGFLWQRRLRRLAEGISEKTETGSEEDRVRNEYEDSRWTGDQDTRSSTVSTTDVEPYYHSMKNFSPQLPPTLEEMSYLRGFTHIEEENMAFPGHLYDEVERLYAPPGAWGPIYDEVPMGPSDPSWPEETYEDTRRIYDRVAGNLDPVETDSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_005616692.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005616692.3

ORF Insert Sequence:

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG TTCCCCCAAC AAACGTGACG ATCTTGGCCA ATGCTACGGC ACTGCGCCCA 
GGGGATGCAT TAAACTTGAC TTGCATCAGT GTTAGCAGCA ACCCGCCTGT CAACTTGTCG 
TGGGACAAGG AGGGGGAGAG GCTGGAAAGT GTGGCCACAC CGCCCCGTAG TGCCCCATTC 
AAAGGCTCTG CGGCAGCCAG GAGCGTCCTT CTGAGAATGT CATCCCGAGA CCACGGCCAC 
CGAGTGACCT GCCAAGCCCA CAGTCCTGAG CTACGGGAAA CCATGAGCGC TTTCTACCGC 
CTCAACGTGC TGTATCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACCGCGGTG 
GAGCAGGGTG AGGCACTGCT GCCGGTGTCT GTGTCTGCCA ACCCTGCCCC GGAGGCCTTC 
AACTGGACTT TCCGTGGCTA CCGTCTCAGC CCAGCTGGCG GCCCTCGGCA TCGCATCTTG 
TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGTGCTG ACGATGGCCT CTATCAGCTG 
CATTGCCAGA ACTCAGAGGG CACCGCCGAA ACTCTAGTGC GGCTGGATGT ACACTATGCT 
CCCACTATCC GAGCTCTCCA GGATCCTACT GAGGTGAATA TTGGGGGTTC TGTGGACATA 
GTCTGCACTG TTGATGCCAA TCCTATCCTT CCAGAGATGT TCAGCTGGGA GAGGCTGGGA 
GAAGAGGAGG AGGACCAGAG CCTGGATGAC ATGGAGAAGA TATCAAAAGG ATCGACAGGG 
CGTCTTCGAA TTCACCATGC CAAACTGGCC CAGGCTGGTG CTTACCAGTG CATCGTGGAT 
AATGGAGTGG CACCTCCAGC CCGAGGGTTG GTCCGACTTG TTGTCAGATT CGCCCCCCAG 
GTGGAGCACC CCACTCCCCT AAACAAGGTG GCTGCAGCTG GAGACAGTAC AAGTTCTGCC 
ACTCTCCACT GTCGTGCCCG AGGCGTCCCC AACATCGTCT TCACTTGGGC CAAAAATGGA 
GTCCCTCTGG ATCTCGAGGA TCCCAGGTAC ACAGAGCACA CATACCACCA GGGTGGGGTC 
CACAGCAGTC TCCTGACCAT TGCCAACGTG TCTGCAGCCC AGGATTATGC CCTCTTCACG 
TGCACAGCCA CCAACCCCCT TGGCTCAGAC CACACCAACA TTCAACTCGT CAGCATTAGC 
CGCCCGGATC CCCCAACAGG ATTAAAGGTT GTAAGCATGA CCCCATATTC GGTGGGGTTG 
GAATGGAAGC CTGGCTTTGA TGGGGGTTTG CCACAGAAGT TCCAAATCAG ATATGAGGCA 
CTTGGGACTC CAGGGTTCCT CTACATGGAC GTCCTACCGC CTGATGCCAC CACCTTCACA 
CTAACTGGGC TACAGCCTTC TACACGATAC AGGGTCTGGC TGCTAGCCAG CAATGCCCTG 
GGGGACAGCG GACTAACTGA CAAGGGGTCC CAGATCCCTA TTACCACCCC AGGTCTAGAC 
GAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGTAGG ACCCTCAGGG 
CTGCCTCTAC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTT TGCTGCTTTC TAATATCTCC 
TGTGTTGGAG GATTCCTCTG GCAGCGGAGA CTGAGGCGTC TTGCTGAAGG CATCTCGGAG 
AAGACAGAGA CAGGGTCGGA GGAGGACCGA GTCAGGAATG AATATGAGGA CAGCCGGTGG 
ACTGGAGACC AGGACACTCG AAGCTCCACA GTTAGCACAA CAGATGTGGA ACCATATTAC 
CACTCCATGA AGAACTTCAG CCCCCAGCTG CCACCAACAC TGGAGGAGAT GTCTTATCTC 
CGAGGTTTCA CACATATTGA AGAGGAGAAT ATGGCTTTTC CTGGACACCT GTATGATGAA 
GTGGAAAGGC TATATGCCCC GCCTGGGGCC TGGGGACCCA TCTATGATGA AGTACCAATG 
GGCCCCTCTG ACCCCTCCTG GCCTGAGGAG ACATATGAGG ATACAAGAAG AATCTATGAT 
CGGGTAGCAG GAAACTTGGA CCCTGTGGAA ACTGATTCTC TACCCTTTGA GCTGAGGGGA 
CATCTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHS1 ( XM_005616692.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005616749.1 Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe02323
Clone ID Related Accession (Same CDS sequence)	XM_005616692.3 , XM_005616692.2
Accession Version	XM_005616692.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	nephrin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG TTCCCCCAAC AAACGTGACG ATCTTGGCCA ATGCTACGGC ACTGCGCCCA 
GGGGATGCAT TAAACTTGAC TTGCATCAGT GTTAGCAGCA ACCCGCCTGT CAACTTGTCG 
TGGGACAAGG AGGGGGAGAG GCTGGAAAGT GTGGCCACAC CGCCCCGTAG TGCCCCATTC 
AAAGGCTCTG CGGCAGCCAG GAGCGTCCTT CTGAGAATGT CATCCCGAGA CCACGGCCAC 
CGAGTGACCT GCCAAGCCCA CAGTCCTGAG CTACGGGAAA CCATGAGCGC TTTCTACCGC 
CTCAACGTGC TGTATCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACCGCGGTG 
GAGCAGGGTG AGGCACTGCT GCCGGTGTCT GTGTCTGCCA ACCCTGCCCC GGAGGCCTTC 
AACTGGACTT TCCGTGGCTA CCGTCTCAGC CCAGCTGGCG GCCCTCGGCA TCGCATCTTG 
TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGTGCTG ACGATGGCCT CTATCAGCTG 
CATTGCCAGA ACTCAGAGGG CACCGCCGAA ACTCTAGTGC GGCTGGATGT ACACTATGCT 
CCCACTATCC GAGCTCTCCA GGATCCTACT GAGGTGAATA TTGGGGGTTC TGTGGACATA 
GTCTGCACTG TTGATGCCAA TCCTATCCTT CCAGAGATGT TCAGCTGGGA GAGGCTGGGA 
GAAGAGGAGG AGGACCAGAG CCTGGATGAC ATGGAGAAGA TATCAAAAGG ATCGACAGGG 
CGTCTTCGAA TTCACCATGC CAAACTGGCC CAGGCTGGTG CTTACCAGTG CATCGTGGAT 
AATGGAGTGG CACCTCCAGC CCGAGGGTTG GTCCGACTTG TTGTCAGATT CGCCCCCCAG 
GTGGAGCACC CCACTCCCCT AAACAAGGTG GCTGCAGCTG GAGACAGTAC AAGTTCTGCC 
ACTCTCCACT GTCGTGCCCG AGGCGTCCCC AACATCGTCT TCACTTGGGC CAAAAATGGA 
GTCCCTCTGG ATCTCGAGGA TCCCAGGTAC ACAGAGCACA CATACCACCA GGGTGGGGTC 
CACAGCAGTC TCCTGACCAT TGCCAACGTG TCTGCAGCCC AGGATTATGC CCTCTTCACG 
TGCACAGCCA CCAACCCCCT TGGCTCAGAC CACACCAACA TTCAACTCGT CAGCATTAGC 
CGCCCGGATC CCCCAACAGG ATTAAAGGTT GTAAGCATGA CCCCATATTC GGTGGGGTTG 
GAATGGAAGC CTGGCTTTGA TGGGGGTTTG CCACAGAAGT TCCAAATCAG ATATGAGGCA 
CTTGGGACTC CAGGGTTCCT CTACATGGAC GTCCTACCGC CTGATGCCAC CACCTTCACA 
CTAACTGGGC TACAGCCTTC TACACGATAC AGGGTCTGGC TGCTAGCCAG CAATGCCCTG 
GGGGACAGCG GACTAACTGA CAAGGGGTCC CAGATCCCTA TTACCACCCC AGGTCTAGAC 
GAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGTAGG ACCCTCAGGG 
CTGCCTCTAC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTT TGCTGCTTTC TAATATCTCC 
TGTGTTGGAG GATTCCTCTG GCAGCGGAGA CTGAGGCGTC TTGCTGAAGG CATCTCGGAG 
AAGACAGAGA CAGGGTCGGA GGAGGACCGA GTCAGGAATG AATATGAGGA CAGCCGGTGG 
ACTGGAGACC AGGACACTCG AAGCTCCACA GTTAGCACAA CAGATGTGGA ACCATATTAC 
CACTCCATGA AGAACTTCAG CCCCCAGCTG CCACCAACAC TGGAGGAGAT GTCTTATCTC 
CGAGGTTTCA CACATATTGA AGAGGAGAAT ATGGCTTTTC CTGGACACCT GTATGATGAA 
GTGGAAAGGC TATATGCCCC GCCTGGGGCC TGGGGACCCA TCTATGATGA AGTACCAATG 
GGCCCCTCTG ACCCCTCCTG GCCTGAGGAG ACATATGAGG ATACAAGAAG AATCTATGAT 
CGGGTAGCAG GAAACTTGGA CCCTGTGGAA ACTGATTCTC TACCCTTTGA GCTGAGGGGA 
CATCTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005616749.1
CDS	2..3853
Translation	MEREENREIEGDEWMKRDNKKSQTHGERDPAKDRTRDRRQAVGTVGATARGPRMALRTPSRAPLLLMGLLTTGLAQLPLSASAPRGFWALPENLTVVEGATAELQCGVHAPGSVVQWAKDGLLLGPDPRIPRFPRYRLEGDPSRGEFHLHIEACDLSDDAEYECQVGRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFFQSGQTVSDISANVNAGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQSLELLCVARGGNPPATLQWLKNGQPMSTAWGTEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKAVPPTNVTILANATALRPGDALNLTCISVSSNPPVNLSWDKEGERLESVATPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAETLVRLDVHYAPTIRALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLGEEEEDQSLDDMEKISKGSTGRLRIHHAKLAQAGAYQCIVDNGVAPPARGLVRLVVRFAPQVEHPTPLNKVAAAGDSTSSATLHCRARGVPNIVFTWAKNGVPLDLEDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTNIQLVSISRPDPPTGLKVVSMTPYSVGLEWKPGFDGGLPQKFQIRYEALGTPGFLYMDVLPPDATTFTLTGLQPSTRYRVWLLASNALGDSGLTDKGSQIPITTPGLDEPSGEPDYQLPTEQPVGPSGLPLLPVLFAVGGLLLLSNISCVGGFLWQRRLRRLAEGISEKTETGSEEDRVRNEYEDSRWTGDQDTRSSTVSTTDVEPYYHSMKNFSPQLPPTLEEMSYLRGFTHIEEENMAFPGHLYDEVERLYAPPGAWGPIYDEVPMGPSDPSWPEETYEDTRRIYDRVAGNLDPVETDSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_005616692.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005616692.2

ORF Insert Sequence:

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG TTCCCCCAAC AAACGTGACG ATCTTGGCCA ATGCTACGGC ACTGCGCCCA 
GGGGATGCAT TAAACTTGAC TTGCATCAGT GTTAGCAGCA ACCCGCCTGT CAACTTGTCG 
TGGGACAAGG AGGGGGAGAG GCTGGAAAGT GTGGCCACAC CGCCCCGTAG TGCCCCATTC 
AAAGGCTCTG CGGCAGCCAG GAGCGTCCTT CTGAGAATGT CATCCCGAGA CCACGGCCAC 
CGAGTGACCT GCCAAGCCCA CAGTCCTGAG CTACGGGAAA CCATGAGCGC TTTCTACCGC 
CTCAACGTGC TGTATCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACCGCGGTG 
GAGCAGGGTG AGGCACTGCT GCCGGTGTCT GTGTCTGCCA ACCCTGCCCC GGAGGCCTTC 
AACTGGACTT TCCGTGGCTA CCGTCTCAGC CCAGCTGGCG GCCCTCGGCA TCGCATCTTG 
TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGTGCTG ACGATGGCCT CTATCAGCTG 
CATTGCCAGA ACTCAGAGGG CACCGCCGAA ACTCTAGTGC GGCTGGATGT ACACTATGCT 
CCCACTATCC GAGCTCTCCA GGATCCTACT GAGGTGAATA TTGGGGGTTC TGTGGACATA 
GTCTGCACTG TTGATGCCAA TCCTATCCTT CCAGAGATGT TCAGCTGGGA GAGGCTGGGA 
GAAGAGGAGG AGGACCAGAG CCTGGATGAC ATGGAGAAGA TATCAAAAGG ATCGACAGGG 
CGTCTTCGAA TTCACCATGC CAAACTGGCC CAGGCTGGTG CTTACCAGTG CATCGTGGAT 
AATGGAGTGG CACCTCCAGC CCGAGGGTTG GTCCGACTTG TTGTCAGATT CGCCCCCCAG 
GTGGAGCACC CCACTCCCCT AAACAAGGTG GCTGCAGCTG GAGACAGTAC AAGTTCTGCC 
ACTCTCCACT GTCGTGCCCG AGGCGTCCCC AACATCGTCT TCACTTGGGC CAAAAATGGA 
GTCCCTCTGG ATCTCGAGGA TCCCAGGTAC ACAGAGCACA CATACCACCA GGGTGGGGTC 
CACAGCAGTC TCCTGACCAT TGCCAACGTG TCTGCAGCCC AGGATTATGC CCTCTTCACG 
TGCACAGCCA CCAACCCCCT TGGCTCAGAC CACACCAACA TTCAACTCGT CAGCATTAGC 
CGCCCGGATC CCCCAACAGG ATTAAAGGTT GTAAGCATGA CCCCATATTC GGTGGGGTTG 
GAATGGAAGC CTGGCTTTGA TGGGGGTTTG CCACAGAAGT TCCAAATCAG ATATGAGGCA 
CTTGGGACTC CAGGGTTCCT CTACATGGAC GTCCTACCGC CTGATGCCAC CACCTTCACA 
CTAACTGGGC TACAGCCTTC TACACGATAC AGGGTCTGGC TGCTAGCCAG CAATGCCCTG 
GGGGACAGCG GACTAACTGA CAAGGGGTCC CAGATCCCTA TTACCACCCC AGGTCTAGAC 
GAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGTAGG ACCCTCAGGG 
CTGCCTCTAC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTT TGCTGCTTTC TAATATCTCC 
TGTGTTGGAG GATTCCTCTG GCAGCGGAGA CTGAGGCGTC TTGCTGAAGG CATCTCGGAG 
AAGACAGAGA CAGGGTCGGA GGAGGACCGA GTCAGGAATG AATATGAGGA CAGCCGGTGG 
ACTGGAGACC AGGACACTCG AAGCTCCACA GTTAGCACAA CAGATGTGGA ACCATATTAC 
CACTCCATGA AGAACTTCAG CCCCCAGCTG CCACCAACAC TGGAGGAGAT GTCTTATCTC 
CGAGGTTTCA CACATATTGA AGAGGAGAAT ATGGCTTTTC CTGGACACCT GTATGATGAA 
GTGGAAAGGC TATATGCCCC GCCTGGGGCC TGGGGACCCA TCTATGATGA AGTACCAATG 
GGCCCCTCTG ACCCCTCCTG GCCTGAGGAG ACATATGAGG ATACAAGAAG AATCTATGAT 
CGGGTAGCAG GAAACTTGGA CCCTGTGGAA ACTGATTCTC TACCCTTTGA GCTGAGGGGA 
CATCTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHS1 ( XM_022403537.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022259245.1 Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X3, mRNA.

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CloneID	OCe286452
Clone ID Related Accession (Same CDS sequence)	XM_022403537.1
Accession Version	XM_022403537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	nephrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC 
GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC 
ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG 
GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC 
GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT 
CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG 
TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG 
GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT 
CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG 
AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC 
GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT 
CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT 
ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG 
GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA 
CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA 
GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC 
AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC 
GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC 
AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC 
AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC 
TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA 
AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG 
GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC 
ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA 
CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG 
GGGTCCCAGA TCCCTATTAC CACCCCAGAG CAATCTTTTT CCTTATGGAG GTCTAGACGA 
GCCTTCTGGA GAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259245.1
CDS	295..3612
Translation	MEREENREIEGDEWMKRDNKKSQTHGERDPAKDRTRDRRQAVGTVGATARGPRMALRTPSRAPLLLMGLLTTGLAQLPLSASAPRGFWALPENLTVVEGATAELQCGVHAPGSVVQWAKDGLLLGPDPRIPRFPRYRLEGDPSRGEFHLHIEACDLSDDAEYECQVGRSETGPELVSPRVILSVLVPPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFFQSGQTVSDISANVNAGSQEKLFTTEATARVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQSLELLCVARGGNPPATLQWLKNGQPMSTAWGTEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKAGQLSASTQLVVQFPPTNVTILANATALRPGDALNLTCISVSSNPPVNLSWDKEGERLESVATPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAETLVRLDVHYAPTIRALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLGEEEEDQSLDDMEKISKGSTGRLRIHHAKLAQAGAYQCIVDNGVAPPARGLVRLVVRFAPQVEHPTPLNKVAAAGDSTSSATLHCRARGVPNIVFTWAKNGVPLDLEDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTNIQLVSISRPDPPTGLKVVSMTPYSVGLEWKPGFDGGLPQKFQIRYEALGTPGFLYMDVLPPDATTFTLTGLQPSTRYRVWLLASNALGDSGLTDKGSQIPITTPEQSFSLWRSRRAFWRT

Target ORF information:

RefSeq Version	XM_022403537.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403537.1

ORF Insert Sequence:

ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG 
GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC 
AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT 
GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC 
ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT 
GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA 
GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA 
GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG 
ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA 
GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG 
GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG 
AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG 
AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT 
ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG 
CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC 
CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA 
GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA 
CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC 
CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA 
TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC 
CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG 
GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA 
CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG 
ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG 
GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC 
ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA 
CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC 
ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG 
TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC 
GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC 
ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG 
GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC 
GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT 
CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG 
TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG 
GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT 
CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG 
AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC 
GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT 
CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT 
ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG 
GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA 
CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA 
GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC 
AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC 
GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC 
AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC 
AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC 
TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA 
AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG 
GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC 
ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA 
CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG 
GGGTCCCAGA TCCCTATTAC CACCCCAGAG CAATCTTTTT CCTTATGGAG GTCTAGACGA 
GCCTTCTGGA GAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHS1 ( XM_022403540.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022259248.1 Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X4, mRNA.

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CloneID	OCe286453
Clone ID Related Accession (Same CDS sequence)	XM_022403540.1
Accession Version	XM_022403540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	nephrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC CCAGGAGAAA CTCTTCACCA CAGAGGCCAC AGCCAGATGA TGACTCTGAC 
TCCAGAACCC CAACTTCCCA CCCCAGGGTG ACACCCCAGA GCTCAGATAA TGGGCAGCTA 
TTGGTCTGTG AGGGGTCCAG CCCAGCATTG GACACCCCTA TCCGGGTCTC GGTCACCATG 
AATGTTCTGT TCCCCCCAGG GCCCCCTGTC ATTGAATGGC CAGGCCTGGA CGAGGGGCAT 
GTGCGGGCAG GACAGAGCTT GGAGCTGCTG TGTGTGGCCC GAGGGGGGAA CCCGCCAGCC 
ACACTGCAGT GGCTGAAGAA TGGCCAGCCC ATGTCCACAG CGTGGGGCAC AGAGCACGCT 
GAGGCAGTGG CCCGAAGCAT GCTAGTGATG GTCGTGCGAC CGGAAGACCA CGGGGCGAGG 
CTCAGCTGTG AGGCCCACAA CAGCGTATCT ACAGGGATCC AGGAGCGCGG GGTCACGCTG 
CAGGTCACCT TTCCCCCTAG TGCCATTACC ATCTTGGGAT CCGCATCCCA GTCTGAGAAC 
AAGAACGTTA CGCTCTCCTG CATCTCCAAG TCCAGTCGCC CACGGGTCCT GTTGCGATGG 
TGGCTGGGTT GGCGGCAGCT GGTGCCCACA GAAGAGATGG TCGTGGATGG CCTGCATGGT 
GGCCACATCT CCATGTCCAA TTTGACGTTC CTGGTGCGAC GGGAGGACAA TGGTCTGACT 
CTCACGTGTG AGGCCTTTAG TGAGGCCTTC ACCAAGGAGA CCTTCAAGAA ATCACTCACC 
CTGAATGTGA AATATCCTGC CCAGAAGTTG TGGATAGAGG GTCCGCCAGA GGGACAACGC 
CTCCGGGCTG GGACTCGAGT GAGGCTGGTG TGTTTGGCCA TTGGAGGCAA CCCAGAACCC 
TCTCTTACTT GGCTCAAGGA CTCCCGGACG GTGACTGAAC CGCGGCCGCC CCAGGAGCCG 
CGGAGGGTGC AGCTGGGAAG TCTGGAGAAG TCAGGGAGCA CGTTCTCCCG AGAGCTTATA 
CTGATCACGG GTCCATCGGA CAACCAGGCC AAGTTCACGT GCAAGGCGGG TCAGCTGAGC 
GCATCCACGC AGCTTGTGGT GCAGTTTCCC CCAACAAACG TGACGATCTT GGCCAATGCT 
ACGGCACTGC GCCCAGGGGA TGCATTAAAC TTGACTTGCA TCAGTGTTAG CAGCAACCCG 
CCTGTCAACT TGTCGTGGGA CAAGGAGGGG GAGAGGCTGG AAAGTGTGGC CACACCGCCC 
CGTAGTGCCC CATTCAAAGG CTCTGCGGCA GCCAGGAGCG TCCTTCTGAG AATGTCATCC 
CGAGACCACG GCCACCGAGT GACCTGCCAA GCCCACAGTC CTGAGCTACG GGAAACCATG 
AGCGCTTTCT ACCGCCTCAA CGTGCTGTAT CCTCCAGAGT TCCTGGGGGA GCAGGTGCTT 
GTGGTGACCG CGGTGGAGCA GGGTGAGGCA CTGCTGCCGG TGTCTGTGTC TGCCAACCCT 
GCCCCGGAGG CCTTCAACTG GACTTTCCGT GGCTACCGTC TCAGCCCAGC TGGCGGCCCT 
CGGCATCGCA TCTTGTCTGG TGGAGCTCTG CAGCTGTGGA ATGTGACCCG TGCTGACGAT 
GGCCTCTATC AGCTGCATTG CCAGAACTCA GAGGGCACCG CCGAAACTCT AGTGCGGCTG 
GATGTACACT ATGCTCCCAC TATCCGAGCT CTCCAGGATC CTACTGAGGT GAATATTGGG 
GGTTCTGTGG ACATAGTCTG CACTGTTGAT GCCAATCCTA TCCTTCCAGA GATGTTCAGC 
TGGGAGAGGC TGGGAGAAGA GGAGGAGGAC CAGAGCCTGG ATGACATGGA GAAGATATCA 
AAAGGATCGA CAGGGCGTCT TCGAATTCAC CATGCCAAAC TGGCCCAGGC TGGTGCTTAC 
CAGTGCATCG TGGATAATGG AGTGGCACCT CCAGCCCGAG GGTTGGTCCG ACTTGTTGTC 
AGATTCGCCC CCCAGGTGGA GCACCCCACT CCCCTAAACA AGGTGGCTGC AGCTGGAGAC 
AGTACAAGTT CTGCCACTCT CCACTGTCGT GCCCGAGGCG TCCCCAACAT CGTCTTCACT 
TGGGCCAAAA ATGGAGTCCC TCTGGATCTC GAGGATCCCA GGTACACAGA GCACACATAC 
CACCAGGGTG GGGTCCACAG CAGTCTCCTG ACCATTGCCA ACGTGTCTGC AGCCCAGGAT 
TATGCCCTCT TCACGTGCAC AGCCACCAAC CCCCTTGGCT CAGACCACAC CAACATTCAA 
CTCGTCAGCA TTAGCCGCCC GGATCCCCCA ACAGGATTAA AGGTTGTAAG CATGACCCCA 
TATTCGGTGG GGTTGGAATG GAAGCCTGGC TTTGATGGGG GTTTGCCACA GAAGTTCCAA 
ATCAGATATG AGGCACTTGG GACTCCAGGG TTCCTCTACA TGGACGTCCT ACCGCCTGAT 
GCCACCACCT TCACACTAAC TGGGCTACAG CCTTCTACAC GATACAGGGT CTGGCTGCTA 
GCCAGCAATG CCCTGGGGGA CAGCGGACTA ACTGACAAGG GGTCCCAGAT CCCTATTACC 
ACCCCAGGTC TAGACGAGCC TTCTGGAGAA CCTGACTACC AGCTGCCCAC AGAGCAGCCT 
GTAGGACCCT CAGGGCTGCC TCTACTGCCT GTGCTGTTTG CTGTTGGGGG TCTTTTGCTG 
CTTTCTAATA TCTCCTGTGT TGGAGGATTC CTCTGGCAGC GGAGACTGAG GCGTCTTGCT 
GAAGGCATCT CGGAGAAGAC AGAGACAGGG TCGGAGGAGG ACCGAGTCAG GAATGAATAT 
GAGGACAGCC GGTGGACTGG AGACCAGGAC ACTCGAAGCT CCACAGTTAG CACAACAGAT 
GTGGAACCAT ATTACCACTC CATGAAGAAC TTCAGCCCCC AGCTGCCACC AACACTGGAG 
GAGATGTCTT ATCTCCGAGG TTTCACACAT ATTGAAGAGG AGAATATGGC TTTTCCTGGA 
CACCTGTATG ATGAAGTGGA AAGGCTATAT GCCCCGCCTG GGGCCTGGGG ACCCATCTAT 
GATGAAGTAC CAATGGGCCC CTCTGACCCC TCCTGGCCTG AGGAGACATA TGAGGATACA 
AGAAGAATCT ATGATCGGGT AGCAGGAAAC TTGGACCCTG TGGAAACTGA TTCTCTACCC 
TTTGAGCTGA GGGGACATCT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259248.1
CDS	124..3330
Translation	MRGPRRNSSPQRPQPDDDSDSRTPTSHPRVTPQSSDNGQLLVCEGSSPALDTPIRVSVTMNVLFPPGPPVIEWPGLDEGHVRAGQSLELLCVARGGNPPATLQWLKNGQPMSTAWGTEHAEAVARSMLVMVVRPEDHGARLSCEAHNSVSTGIQERGVTLQVTFPPSAITILGSASQSENKNVTLSCISKSSRPRVLLRWWLGWRQLVPTEEMVVDGLHGGHISMSNLTFLVRREDNGLTLTCEAFSEAFTKETFKKSLTLNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPEPSLTWLKDSRTVTEPRPPQEPRRVQLGSLEKSGSTFSRELILITGPSDNQAKFTCKAGQLSASTQLVVQFPPTNVTILANATALRPGDALNLTCISVSSNPPVNLSWDKEGERLESVATPPRSAPFKGSAAARSVLLRMSSRDHGHRVTCQAHSPELRETMSAFYRLNVLYPPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGLYQLHCQNSEGTAETLVRLDVHYAPTIRALQDPTEVNIGGSVDIVCTVDANPILPEMFSWERLGEEEEDQSLDDMEKISKGSTGRLRIHHAKLAQAGAYQCIVDNGVAPPARGLVRLVVRFAPQVEHPTPLNKVAAAGDSTSSATLHCRARGVPNIVFTWAKNGVPLDLEDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTNIQLVSISRPDPPTGLKVVSMTPYSVGLEWKPGFDGGLPQKFQIRYEALGTPGFLYMDVLPPDATTFTLTGLQPSTRYRVWLLASNALGDSGLTDKGSQIPITTPGLDEPSGEPDYQLPTEQPVGPSGLPLLPVLFAVGGLLLLSNISCVGGFLWQRRLRRLAEGISEKTETGSEEDRVRNEYEDSRWTGDQDTRSSTVSTTDVEPYYHSMKNFSPQLPPTLEEMSYLRGFTHIEEENMAFPGHLYDEVERLYAPPGAWGPIYDEVPMGPSDPSWPEETYEDTRRIYDRVAGNLDPVETDSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_022403540.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403540.1

ORF Insert Sequence:

ATGCGGGGTC CCAGGAGAAA CTCTTCACCA CAGAGGCCAC AGCCAGATGA TGACTCTGAC 
TCCAGAACCC CAACTTCCCA CCCCAGGGTG ACACCCCAGA GCTCAGATAA TGGGCAGCTA 
TTGGTCTGTG AGGGGTCCAG CCCAGCATTG GACACCCCTA TCCGGGTCTC GGTCACCATG 
AATGTTCTGT TCCCCCCAGG GCCCCCTGTC ATTGAATGGC CAGGCCTGGA CGAGGGGCAT 
GTGCGGGCAG GACAGAGCTT GGAGCTGCTG TGTGTGGCCC GAGGGGGGAA CCCGCCAGCC 
ACACTGCAGT GGCTGAAGAA TGGCCAGCCC ATGTCCACAG CGTGGGGCAC AGAGCACGCT 
GAGGCAGTGG CCCGAAGCAT GCTAGTGATG GTCGTGCGAC CGGAAGACCA CGGGGCGAGG 
CTCAGCTGTG AGGCCCACAA CAGCGTATCT ACAGGGATCC AGGAGCGCGG GGTCACGCTG 
CAGGTCACCT TTCCCCCTAG TGCCATTACC ATCTTGGGAT CCGCATCCCA GTCTGAGAAC 
AAGAACGTTA CGCTCTCCTG CATCTCCAAG TCCAGTCGCC CACGGGTCCT GTTGCGATGG 
TGGCTGGGTT GGCGGCAGCT GGTGCCCACA GAAGAGATGG TCGTGGATGG CCTGCATGGT 
GGCCACATCT CCATGTCCAA TTTGACGTTC CTGGTGCGAC GGGAGGACAA TGGTCTGACT 
CTCACGTGTG AGGCCTTTAG TGAGGCCTTC ACCAAGGAGA CCTTCAAGAA ATCACTCACC 
CTGAATGTGA AATATCCTGC CCAGAAGTTG TGGATAGAGG GTCCGCCAGA GGGACAACGC 
CTCCGGGCTG GGACTCGAGT GAGGCTGGTG TGTTTGGCCA TTGGAGGCAA CCCAGAACCC 
TCTCTTACTT GGCTCAAGGA CTCCCGGACG GTGACTGAAC CGCGGCCGCC CCAGGAGCCG 
CGGAGGGTGC AGCTGGGAAG TCTGGAGAAG TCAGGGAGCA CGTTCTCCCG AGAGCTTATA 
CTGATCACGG GTCCATCGGA CAACCAGGCC AAGTTCACGT GCAAGGCGGG TCAGCTGAGC 
GCATCCACGC AGCTTGTGGT GCAGTTTCCC CCAACAAACG TGACGATCTT GGCCAATGCT 
ACGGCACTGC GCCCAGGGGA TGCATTAAAC TTGACTTGCA TCAGTGTTAG CAGCAACCCG 
CCTGTCAACT TGTCGTGGGA CAAGGAGGGG GAGAGGCTGG AAAGTGTGGC CACACCGCCC 
CGTAGTGCCC CATTCAAAGG CTCTGCGGCA GCCAGGAGCG TCCTTCTGAG AATGTCATCC 
CGAGACCACG GCCACCGAGT GACCTGCCAA GCCCACAGTC CTGAGCTACG GGAAACCATG 
AGCGCTTTCT ACCGCCTCAA CGTGCTGTAT CCTCCAGAGT TCCTGGGGGA GCAGGTGCTT 
GTGGTGACCG CGGTGGAGCA GGGTGAGGCA CTGCTGCCGG TGTCTGTGTC TGCCAACCCT 
GCCCCGGAGG CCTTCAACTG GACTTTCCGT GGCTACCGTC TCAGCCCAGC TGGCGGCCCT 
CGGCATCGCA TCTTGTCTGG TGGAGCTCTG CAGCTGTGGA ATGTGACCCG TGCTGACGAT 
GGCCTCTATC AGCTGCATTG CCAGAACTCA GAGGGCACCG CCGAAACTCT AGTGCGGCTG 
GATGTACACT ATGCTCCCAC TATCCGAGCT CTCCAGGATC CTACTGAGGT GAATATTGGG 
GGTTCTGTGG ACATAGTCTG CACTGTTGAT GCCAATCCTA TCCTTCCAGA GATGTTCAGC 
TGGGAGAGGC TGGGAGAAGA GGAGGAGGAC CAGAGCCTGG ATGACATGGA GAAGATATCA 
AAAGGATCGA CAGGGCGTCT TCGAATTCAC CATGCCAAAC TGGCCCAGGC TGGTGCTTAC 
CAGTGCATCG TGGATAATGG AGTGGCACCT CCAGCCCGAG GGTTGGTCCG ACTTGTTGTC 
AGATTCGCCC CCCAGGTGGA GCACCCCACT CCCCTAAACA AGGTGGCTGC AGCTGGAGAC 
AGTACAAGTT CTGCCACTCT CCACTGTCGT GCCCGAGGCG TCCCCAACAT CGTCTTCACT 
TGGGCCAAAA ATGGAGTCCC TCTGGATCTC GAGGATCCCA GGTACACAGA GCACACATAC 
CACCAGGGTG GGGTCCACAG CAGTCTCCTG ACCATTGCCA ACGTGTCTGC AGCCCAGGAT 
TATGCCCTCT TCACGTGCAC AGCCACCAAC CCCCTTGGCT CAGACCACAC CAACATTCAA 
CTCGTCAGCA TTAGCCGCCC GGATCCCCCA ACAGGATTAA AGGTTGTAAG CATGACCCCA 
TATTCGGTGG GGTTGGAATG GAAGCCTGGC TTTGATGGGG GTTTGCCACA GAAGTTCCAA 
ATCAGATATG AGGCACTTGG GACTCCAGGG TTCCTCTACA TGGACGTCCT ACCGCCTGAT 
GCCACCACCT TCACACTAAC TGGGCTACAG CCTTCTACAC GATACAGGGT CTGGCTGCTA 
GCCAGCAATG CCCTGGGGGA CAGCGGACTA ACTGACAAGG GGTCCCAGAT CCCTATTACC 
ACCCCAGGTC TAGACGAGCC TTCTGGAGAA CCTGACTACC AGCTGCCCAC AGAGCAGCCT 
GTAGGACCCT CAGGGCTGCC TCTACTGCCT GTGCTGTTTG CTGTTGGGGG TCTTTTGCTG 
CTTTCTAATA TCTCCTGTGT TGGAGGATTC CTCTGGCAGC GGAGACTGAG GCGTCTTGCT 
GAAGGCATCT CGGAGAAGAC AGAGACAGGG TCGGAGGAGG ACCGAGTCAG GAATGAATAT 
GAGGACAGCC GGTGGACTGG AGACCAGGAC ACTCGAAGCT CCACAGTTAG CACAACAGAT 
GTGGAACCAT ATTACCACTC CATGAAGAAC TTCAGCCCCC AGCTGCCACC AACACTGGAG 
GAGATGTCTT ATCTCCGAGG TTTCACACAT ATTGAAGAGG AGAATATGGC TTTTCCTGGA 
CACCTGTATG ATGAAGTGGA AAGGCTATAT GCCCCGCCTG GGGCCTGGGG ACCCATCTAT 
GATGAAGTAC CAATGGGCCC CTCTGACCCC TCCTGGCCTG AGGAGACATA TGAGGATACA 
AGAAGAATCT ATGATCGGGT AGCAGGAAAC TTGGACCCTG TGGAAACTGA TTCTCTACCC 
TTTGAGCTGA GGGGACATCT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NPHS1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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