nphs1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nphs1
Entrez Gene ID	100494620
Full Name	NPHS1 nephrin
Synonyms	nephrin
General protein information	Preferred Names NPHS1 nephrin Names nephrin nephrosis 1, congenital, Finnish type (nephrin)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031905692.1	XP_031761552.1	nephrin
XM_018089423.1	XP_017944912.1	nephrin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPHS1	XP_524228.3
Homo sapiens (human)	NPHS1	NP_004637.1
Rattus norvegicus (Norway rat)	Nphs1	NP_072150.1
Mus musculus (house mouse)	Nphs1	NP_062332.2
Drosophila melanogaster (fruit fly)	sns	NP_788286.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010742	XP_311455.4
Macaca mulatta (Rhesus monkey)	NPHS1	XP_001112314.1
Canis lupus familiaris (dog)	NPHS1	XP_541685.3
Bos taurus (cattle)	NPHS1	NP_001179441.1
Danio rerio (zebrafish)	nphs1	NP_001035777.1
Xenopus tropicalis (tropical clawed frog)	nphs1	XP_002940674.2

Gene Ontology

The following nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa40681	XM_018089423.1 Latest version!	Xenopus tropicalis nephrosis 1, congenital, Finnish type (nephrin) (nphs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62093	XM_031905692.1 Latest version!	Xenopus tropicalis NPHS1 nephrin (nphs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nphs1 ( XM_018089423.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944912.1 Xenopus tropicalis nephrosis 1, congenital, Finnish type (nephrin) (nphs1), mRNA.

Price & Availability↑

CloneID	OXa40681
Clone ID Related Accession (Same CDS sequence)	XM_018089423.1
Accession Version	XM_018089423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nephrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGCTGCCTG TACTGTTTGC CGCTTGTTGC AGTTGGTGGA TCTTAAGGGA AGGCGTGACG 
GCTAAGCAGC AGGTGTTCCG AGTGCAGCCG GACAATATCA CCGTCGTGCA GGGATCGACC 
GCACTGCTGA GTTGTGAGGT GGAACATGGG ACTGGTATGG TGCAGTGGGA AAAGAATGGA 
CTCCTGCTGG GACCTGACAG GAATATCCCA GGGTTCCTGC GCTACAGCAT GACTGGAGAC 
CCACACAAAG GAGAGTACAA CCTGCGCATT GAGCGTAGCG AGCTGGATGA CGACGCCGAG 
TATCACTGTC AGGTGGGGAG ATCAGAGGTC AGCTTGGGCA TCATTTCCCG CACTGCTCTC 
ATCAACGTCC TCATTCCTCC CAAAGCACCC ACTTTTGAAG AGTATGCTGC GGGCAGCCGA 
GTGACTTGGG TTTACGGGGT GGAATATACA GTCACCTGCC AAGCAACTGA CGCCAAACCG 
GCTGCTGCCT TGACCATCAA AAAGAGTGGA CTGGAGGTGT CTGGAGATTC ATCTTCAAAC 
CCAGGATCCA GAGACAAATT GGAGAACACA GCAATGGTGG CAAAGGTCAT CCCCCAGAGC 
TCTGATAATG GGAAACTGCT GACCTGCTTG GCGCTAAATC CAGCTCTTCA GACGCCAATC 
ACCGTGAGCT TCACCATGAA TGTGCTGTTT CCGCCTAATG CCCCGATTAT AGAAGGTTAT 
ATTGGGCCAT CGGTTAAAGC TGGGGAAACA CTGAAGCTCA TCTGCATTTC TTTAAGTGGA 
AACCCCCTGG CCACATTACA ATGGTCAAAG AACGATGATG TTGTGTCAAC ACGGTGGGAG 
ACGGATGAGG TGGTCCGAGC TTCTCGTAGT CACCTGACCC TGAATATTAA GCCTGCGGAT 
AACATGGCGG TTGTGAGCTG TGAGGCTGTA AATCATGTGA CTCCAGAGAC TCTCAAAACC 
AGCATCATCC TCAATGTTGT GTTTCTGCCA ACAGAAGTAA CCATCCTTGG CTCATCAAGT 
GGCCCAGAGA AATCTAATCT AACGTTCTCC TGCTTTACGG CTGCCAGTAA CCCTCCTGTA 
CAGATCCGCT GGTGGCTGGG CCCAAAGGAA CTAGTGAACA CCATTGTCAC CATCTCTGAT 
GCTGTTCATG GTGGAAAGGT GACAATGTCC AACCTCACTA TGGAGGCTCT TCGAGAGGAG 
CATGGAATGG CGTTGACCTG TGAGGCCTTT AATGAGGCCA TTCTCTACAC CAAATCCAAC 
AGCGTTACCC TAAGTGTGCA ATACCCTCCA CAGAATGTCT GGATCGAGGC CCCTCCAGCT 
GATAAGTTCT TCCGTGCGGG GACATCAGTG AAGATGACTT GTTTCTCTAG CGGTGGAAAC 
CCGCCACCCA GGCTTACCTG GATAAAGGAT ACTAAAAGCC TGAGTGGGGG ATCGCAGATC 
CATTCAGGAA AAATTGTCAC AAAAGAAATT ACAATTATAA CTGTGCCAAG TGACAATATG 
GCCACATACC GGTGCAACGC CAGCAACATT GGGAAGACGC CGGCGCTGAC TGCCAGCACG 
AAACTAAAGG TCCAGTTTCC TGCCATAGAT GTGAATATAA TTAGCAGCGC CAAAGAGTAT 
CGCCGCGGTT CTACCATTAT ACTGACCTGT GTCACCGGCA GCAGTAACCC TGCATCTACT 
ATATCCTGGG TGAAAGATGG AGAGCAGTTA AAAGCTCAGG ATTTGGGAAG AAAACCTTCT 
CTGTATGGGG GAATTTCCAG TTCCAGTCGT GTCACACTGA AAGCAGCATC TAAGGACAAC 
GGAAGGCGCG TCTCCTGCGA GGCGTTCAGC AGCGTCCTCA ACGAGGCCGT CAACACCTTC 
TACAAACTCA ATATCCTGTT TCCCCCTGAG TTTCTGGATG ATCAGCCGGC GGTGGTACAG 
GCCGTGGAGC ATGGAGCTGC CCTTATCCCA GTCCGGGTCA GGGCCAACCC ACCTCAGATC 
AACTACACGT GGAGCCTTTG GGGCGAGAAA CTGATCCGAG ATGGTGCTTA CCGACATCAC 
CTGCGCGGTG AGGGGGCCCT CGAGATATGG AACGTGAGTC GCGCTGACTC CGGGGTCTAC 
AACGTGACTT GTGTGAATAA AGAGGGGAAG AACAGCATTG TAATAAGACT GGATGTGCAG 
TATTCTCCCA CCATTCGAAG CCTTGGGGAC ACAGAGGTGG ATCTCGGTTC AGACGCAGAG 
ATCGCTTGCA CGGCAGATGC GAATCCAGCG ACAGACTCAA TGTTCCAGTG GAGATGGCTG 
GTCTGGATCC GTTTATATTG CTCTGTGATC ATTACCATTA GACCTTATGT TGGTTTCCAG 
TTAAAGGGGC TCTTTTACCA ATGCGAGTGC AAAGCGCTAC AATGGATACA AAATGGCGCA 
TATCTGGATG TAGTTAAGCC TGATATACAC AAGGGGGTGC ACTTGAGCAA AGTGGCAGTT 
CCTGGTGATG GGAAGAGCGT GGCAGCCTTG GTGTGTAAAG CCGAGGGAAT CCCGAGTGTG 
GCGTTTAGCT GGGCTAAGAA TGGGGTATCT CTGGATCTCA AAGATCCCAG GTACTCAGAG 
AAGACATTTC ATGAGCTGTC AGTTCACACC AGCACGCTGG TCATTTCCAA TGTGAGCGCA 
GTGAAGGACT ACGCCCTCTT CACGTGTACG GCCACGAACA CCCTGGGGGT CGACAGTTTC 
ACCATTCAGC TTGTCAGCCC CAGCCGTCCT GACCCTCCTT CAGGTCTGAG AGTCATCGGT 
TTCACTCACA ACTGGGTGAC GCTACAATGG AGTGCTGGAT TTGATGGAGG GGCGGAGCAA 
AAATTTCGAG TCAGATCCTT TTTTTTTTTT TTTAAACTTT TTTACCCAGA AGCCCTTGAG 
TGGCCACCAT ACATATTAGC AGCTGTGTGT GCCGGAGGTG CCCTCCTTCT GGTCTCCAAT 
GCGGCTTTTA TTGGGTGCCT CGTGAAAAGA AGCAGAGACA AGTCCCAAGG AGGAGAAGGT 
GTGAAGAAGG ATGAGAGGAA TGTTTCTCTA TGTTTGCGCT TCTCAACCAA ACAGGGCGGC 
TATGAAGACT GGTATTCGAC TTCCGAAGGC CATGAGTATG AAGAAGTAGG AGATTACCCA 
CAATCCTCTA GAGTCCGATG GCAGAACAGA GAAACGGCCA ATCAGGGAAG AGAGGCATCG 
TGGGCAGAAT ACCAGGATCC AGGCAGATTA AGAATCTATG ACGAGGCAGA CGTGACCTTG 
TCTGACGTGT ACGAGACTCC ATATGGGGGG CGGGGGCAAC TAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944912.1
CDS	166..3453
Translation	MLPVLFAACCSWWILREGVTAKQQVFRVQPDNITVVQGSTALLSCEVEHGTGMVQWEKNGLLLGPDRNIPGFLRYSMTGDPHKGEYNLRIERSELDDDAEYHCQVGRSEVSLGIISRTALINVLIPPKAPTFEEYAAGSRVTWVYGVEYTVTCQATDAKPAAALTIKKSGLEVSGDSSSNPGSRDKLENTAMVAKVIPQSSDNGKLLTCLALNPALQTPITVSFTMNVLFPPNAPIIEGYIGPSVKAGETLKLICISLSGNPLATLQWSKNDDVVSTRWETDEVVRASRSHLTLNIKPADNMAVVSCEAVNHVTPETLKTSIILNVVFLPTEVTILGSSSGPEKSNLTFSCFTAASNPPVQIRWWLGPKELVNTIVTISDAVHGGKVTMSNLTMEALREEHGMALTCEAFNEAILYTKSNSVTLSVQYPPQNVWIEAPPADKFFRAGTSVKMTCFSSGGNPPPRLTWIKDTKSLSGGSQIHSGKIVTKEITIITVPSDNMATYRCNASNIGKTPALTASTKLKVQFPAIDVNIISSAKEYRRGSTIILTCVTGSSNPASTISWVKDGEQLKAQDLGRKPSLYGGISSSSRVTLKAASKDNGRRVSCEAFSSVLNEAVNTFYKLNILFPPEFLDDQPAVVQAVEHGAALIPVRVRANPPQINYTWSLWGEKLIRDGAYRHHLRGEGALEIWNVSRADSGVYNVTCVNKEGKNSIVIRLDVQYSPTIRSLGDTEVDLGSDAEIACTADANPATDSMFQWRWLVWIRLYCSVIITIRPYVGFQLKGLFYQCECKALQWIQNGAYLDVVKPDIHKGVHLSKVAVPGDGKSVAALVCKAEGIPSVAFSWAKNGVSLDLKDPRYSEKTFHELSVHTSTLVISNVSAVKDYALFTCTATNTLGVDSFTIQLVSPSRPDPPSGLRVIGFTHNWVTLQWSAGFDGGAEQKFRVRSFFFFFKLFYPEALEWPPYILAAVCAGGALLLVSNAAFIGCLVKRSRDKSQGGEGVKKDERNVSLCLRFSTKQGGYEDWYSTSEGHEYEEVGDYPQSSRVRWQNRETANQGREASWAEYQDPGRLRIYDEADVTLSDVYETPYGGRGQLV

Target ORF information:

RefSeq Version	XM_018089423.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nephrosis 1, congenital, Finnish type (nephrin) (nphs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089423.1

ORF Insert Sequence:

ATGCTGCCTG TACTGTTTGC CGCTTGTTGC AGTTGGTGGA TCTTAAGGGA AGGCGTGACG 
GCTAAGCAGC AGGTGTTCCG AGTGCAGCCG GACAATATCA CCGTCGTGCA GGGATCGACC 
GCACTGCTGA GTTGTGAGGT GGAACATGGG ACTGGTATGG TGCAGTGGGA AAAGAATGGA 
CTCCTGCTGG GACCTGACAG GAATATCCCA GGGTTCCTGC GCTACAGCAT GACTGGAGAC 
CCACACAAAG GAGAGTACAA CCTGCGCATT GAGCGTAGCG AGCTGGATGA CGACGCCGAG 
TATCACTGTC AGGTGGGGAG ATCAGAGGTC AGCTTGGGCA TCATTTCCCG CACTGCTCTC 
ATCAACGTCC TCATTCCTCC CAAAGCACCC ACTTTTGAAG AGTATGCTGC GGGCAGCCGA 
GTGACTTGGG TTTACGGGGT GGAATATACA GTCACCTGCC AAGCAACTGA CGCCAAACCG 
GCTGCTGCCT TGACCATCAA AAAGAGTGGA CTGGAGGTGT CTGGAGATTC ATCTTCAAAC 
CCAGGATCCA GAGACAAATT GGAGAACACA GCAATGGTGG CAAAGGTCAT CCCCCAGAGC 
TCTGATAATG GGAAACTGCT GACCTGCTTG GCGCTAAATC CAGCTCTTCA GACGCCAATC 
ACCGTGAGCT TCACCATGAA TGTGCTGTTT CCGCCTAATG CCCCGATTAT AGAAGGTTAT 
ATTGGGCCAT CGGTTAAAGC TGGGGAAACA CTGAAGCTCA TCTGCATTTC TTTAAGTGGA 
AACCCCCTGG CCACATTACA ATGGTCAAAG AACGATGATG TTGTGTCAAC ACGGTGGGAG 
ACGGATGAGG TGGTCCGAGC TTCTCGTAGT CACCTGACCC TGAATATTAA GCCTGCGGAT 
AACATGGCGG TTGTGAGCTG TGAGGCTGTA AATCATGTGA CTCCAGAGAC TCTCAAAACC 
AGCATCATCC TCAATGTTGT GTTTCTGCCA ACAGAAGTAA CCATCCTTGG CTCATCAAGT 
GGCCCAGAGA AATCTAATCT AACGTTCTCC TGCTTTACGG CTGCCAGTAA CCCTCCTGTA 
CAGATCCGCT GGTGGCTGGG CCCAAAGGAA CTAGTGAACA CCATTGTCAC CATCTCTGAT 
GCTGTTCATG GTGGAAAGGT GACAATGTCC AACCTCACTA TGGAGGCTCT TCGAGAGGAG 
CATGGAATGG CGTTGACCTG TGAGGCCTTT AATGAGGCCA TTCTCTACAC CAAATCCAAC 
AGCGTTACCC TAAGTGTGCA ATACCCTCCA CAGAATGTCT GGATCGAGGC CCCTCCAGCT 
GATAAGTTCT TCCGTGCGGG GACATCAGTG AAGATGACTT GTTTCTCTAG CGGTGGAAAC 
CCGCCACCCA GGCTTACCTG GATAAAGGAT ACTAAAAGCC TGAGTGGGGG ATCGCAGATC 
CATTCAGGAA AAATTGTCAC AAAAGAAATT ACAATTATAA CTGTGCCAAG TGACAATATG 
GCCACATACC GGTGCAACGC CAGCAACATT GGGAAGACGC CGGCGCTGAC TGCCAGCACG 
AAACTAAAGG TCCAGTTTCC TGCCATAGAT GTGAATATAA TTAGCAGCGC CAAAGAGTAT 
CGCCGCGGTT CTACCATTAT ACTGACCTGT GTCACCGGCA GCAGTAACCC TGCATCTACT 
ATATCCTGGG TGAAAGATGG AGAGCAGTTA AAAGCTCAGG ATTTGGGAAG AAAACCTTCT 
CTGTATGGGG GAATTTCCAG TTCCAGTCGT GTCACACTGA AAGCAGCATC TAAGGACAAC 
GGAAGGCGCG TCTCCTGCGA GGCGTTCAGC AGCGTCCTCA ACGAGGCCGT CAACACCTTC 
TACAAACTCA ATATCCTGTT TCCCCCTGAG TTTCTGGATG ATCAGCCGGC GGTGGTACAG 
GCCGTGGAGC ATGGAGCTGC CCTTATCCCA GTCCGGGTCA GGGCCAACCC ACCTCAGATC 
AACTACACGT GGAGCCTTTG GGGCGAGAAA CTGATCCGAG ATGGTGCTTA CCGACATCAC 
CTGCGCGGTG AGGGGGCCCT CGAGATATGG AACGTGAGTC GCGCTGACTC CGGGGTCTAC 
AACGTGACTT GTGTGAATAA AGAGGGGAAG AACAGCATTG TAATAAGACT GGATGTGCAG 
TATTCTCCCA CCATTCGAAG CCTTGGGGAC ACAGAGGTGG ATCTCGGTTC AGACGCAGAG 
ATCGCTTGCA CGGCAGATGC GAATCCAGCG ACAGACTCAA TGTTCCAGTG GAGATGGCTG 
GTCTGGATCC GTTTATATTG CTCTGTGATC ATTACCATTA GACCTTATGT TGGTTTCCAG 
TTAAAGGGGC TCTTTTACCA ATGCGAGTGC AAAGCGCTAC AATGGATACA AAATGGCGCA 
TATCTGGATG TAGTTAAGCC TGATATACAC AAGGGGGTGC ACTTGAGCAA AGTGGCAGTT 
CCTGGTGATG GGAAGAGCGT GGCAGCCTTG GTGTGTAAAG CCGAGGGAAT CCCGAGTGTG 
GCGTTTAGCT GGGCTAAGAA TGGGGTATCT CTGGATCTCA AAGATCCCAG GTACTCAGAG 
AAGACATTTC ATGAGCTGTC AGTTCACACC AGCACGCTGG TCATTTCCAA TGTGAGCGCA 
GTGAAGGACT ACGCCCTCTT CACGTGTACG GCCACGAACA CCCTGGGGGT CGACAGTTTC 
ACCATTCAGC TTGTCAGCCC CAGCCGTCCT GACCCTCCTT CAGGTCTGAG AGTCATCGGT 
TTCACTCACA ACTGGGTGAC GCTACAATGG AGTGCTGGAT TTGATGGAGG GGCGGAGCAA 
AAATTTCGAG TCAGATCCTT TTTTTTTTTT TTTAAACTTT TTTACCCAGA AGCCCTTGAG 
TGGCCACCAT ACATATTAGC AGCTGTGTGT GCCGGAGGTG CCCTCCTTCT GGTCTCCAAT 
GCGGCTTTTA TTGGGTGCCT CGTGAAAAGA AGCAGAGACA AGTCCCAAGG AGGAGAAGGT 
GTGAAGAAGG ATGAGAGGAA TGTTTCTCTA TGTTTGCGCT TCTCAACCAA ACAGGGCGGC 
TATGAAGACT GGTATTCGAC TTCCGAAGGC CATGAGTATG AAGAAGTAGG AGATTACCCA 
CAATCCTCTA GAGTCCGATG GCAGAACAGA GAAACGGCCA ATCAGGGAAG AGAGGCATCG 
TGGGCAGAAT ACCAGGATCC AGGCAGATTA AGAATCTATG ACGAGGCAGA CGTGACCTTG 
TCTGACGTGT ACGAGACTCC ATATGGGGGG CGGGGGCAAC TAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphs1 ( XM_031905692.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761552.1 Xenopus tropicalis NPHS1 nephrin (nphs1), mRNA.

Price & Availability↑

CloneID	OXa62093
Clone ID Related Accession (Same CDS sequence)	XM_031905692.1
Accession Version	XM_031905692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nephrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCTG TACTGTTTGC CGCTTGTTGC AGTTGGTGGA TCTTAAGGGA AGGCGTGACG 
GCTAAGCAGC AGGTGTTCCG AGTGCAGCCG GACAATATCA CCGTCGTGCA GGGATCGACC 
GCACTGCTGA GTTGTGAGGT GGAACATGGG ACTGGTATGG TGCAGTGGGA AAAGAATGGA 
CTCCTGCTGG GACCTGACAG GAATATCCCA GGGTTCCTGC GCTACAGCAT GACTGGAGAC 
CCACACAAAG GAGAGTACAA CCTGCGCATT GAGCGTAGCG AGCTGGATGA CGACGCCGAG 
TATCACTGTC AGGTGGGGAG ATCAGAGGTC AGCTTGGGCA TCATTTCCCG CACTGCTCTC 
ATCAACGTCC TCATTCCTCC CAAAGCACCC ACTTTTGAAG AGTATGCTGC GGGCAGCCGA 
GTGACTTGGG TTTACGGGGT GGAATATACA GTCACCTGCC AAGCAACTGA CGCCAAACCG 
GCTGCTGCCT TGACCATCAA AAAGAGTGGA CTGGAGGTGT CTGGAGATTC ATCTTCAAAC 
CCAGGATCCA GAGACAAATT GGAGAACACA GCAATGGTGG CAAAGGTCAT CCCCCAGAGC 
TCTGATAATG GGAAACTGCT GACCTGCTTG GCGCTAAATC CAGCTCTTCA GACGCCAATC 
ACCGTGAGCT TCACCATGAA TGTGCTGTTT CCGCCTAATG CCCCGATTAT AGAAGGTTAT 
ATTGGGCCAT CGGTTAAAGC TGGGGAAACA CTGAAGCTCA TCTGCATTTC TTTAAGTGGA 
AACCCCCTGG CCACATTACA ATGGTCAAAG AACGATGATG TTGTGTCAAC ACGGTGGGAG 
ACGGATGAGG TGGTCCGAGC TTCTCGTAGT CACCTGACCC TGAATATTAA GCCTGCGGAT 
AACATGGCGG TTGTGAGCTG TGAGGCTGTA AATCATGTGA CTCCAGAGAC TCTCAAAACC 
AGCATCATCC TCAATGTTGT GTTTCTGCCA ACAGAAGTAA CCATCCTTGG CTCATCAAGT 
GGCCCAGAGA AATCTAATCT AACGTTCTCC TGCTTTACGG CTGCCAGTAA CCCTCCTGTA 
CAGATCCGCT GGTGGCTGGG CCCAAAGGAA CTAGTGAACA CCATTGTCAC CATCTCTGAT 
GCTGTTCATG GTGGAAAGGT GACAATGTCC AACCTCACTA TGGAGGCTCT TCGAGAGGAG 
CATGGAATGG CGTTGACCTG TGAGGCCTTT AATGAGGCCA TTCTCTACAC CAAATCCAAC 
AGCGTTACCC TAAGTGTGCA ATACCCTCCA CAGAATGTCT GGATCGAGGC CCCTCCAGCT 
GATAAGTTCT TCCGTGCGGG GACATCAGTG AAGATGACTT GTTTCTCTAG CGGTGGAAAC 
CCGCCACCCA GGCTTACCTG GATAAAGGAT ACTAAAAGCC TGAGTGGGGG ATCGCAGATC 
CATTCAGGAA AAATTGTCAC AAAAGAAATT ACAATTATAA CTGTGCCAAG TGACAATATG 
GCCACATACC GGTGCAACGC CAGCAACATT GGGAAGACGC CGGCGCTGAC TGCCAGCACG 
AAACTAAAGG TCCAGTTTCC TGCCATAGAT GTGAATATAA TTAGCAGCGC CAAAGAGTAT 
CGCCGCGGTT CTACCATTAT ACTGACCTGT GTCACCGGCA GCAGTAACCC TGCATCTACT 
ATATCCTGGG TGAAAGATGG AGAGCAGTTA AAAGCTCAGG ATTTGGGAAG AAAACCATCT 
CTGTATGGGG GAATTTCCAG TTCCAGTCGT GTCACACTGA AAGCAGCATC TAAGGACAAC 
GGAAGGCGCG TCTCCTGCGA GGCGTTCAGC AGCGTCCTCA ACGAGGCCGT CAACACCTTC 
TACAAACTCA ATATCCTGTT TCCCCCTGAG TTTCTGGATG ATCAGCCGGC GGTGGTGCAG 
GCCGTGGAGC ATGGAGCTGC CCTTATCCCA GTCCGGGTCA GGGCCAACCC ACCTCAGATC 
AACTACACGT GGAGCCTTTG GGGCGAGAAA CTGATCCGAG ATGGTGCTTA CCGACATCAC 
CTGCGCGGTG AGGGGGCCCT CGAGATATGG AACGTGAGTC GCGCTGACTC CGGGGTCTAC 
AATGTGACTT GTGTGAATAA AGAGGGGAAG AACAGCATTG TAATAAGACT GGATGTGCAG 
TATTCTCCCA CCATTCGAAG CCTTGGGGAC ACAGAGGTGG ATCTCGGTTC AGACGCAGAG 
ATCGCTTGCA CGGCAGATGC GAATCCAGCG ACAGACTCAA TGTTCCAGTG GAGATGGCTG 
GGTGATGAGG AGCGAGACCT CTCTGCCTTG GAACGCAAAG TGGATGGAGT CATTGGACGC 
CTGGTTATCC GGGAAGCCAA GAGAACGGAC GCCGGCCGCT ACGAGTGCAC CGTGGATAAC 
GGGATCCCCC CCTCCATCAA GGCTGATGCT CGTCTGATTG TCCGATTTAA GCCTGATATA 
CACAAGGGGG TGCACTTGAG CAAAGTGGCA GTTCCTGGTG ATGGGAAGAG CGTGGCAGCC 
TTGGTGTGTA AAGCTGAGGG AATCCCGAGT GTGGCGTTTA GCTGGGCTAA GAATGGGGTA 
TCTCTGGATC TCAAAGATCC CAGGTACTCA GAGAAGACAT TTCATGAGCT GTCAGTTCAC 
ACCAGCACGC TGGTCATTTC CAATGTGAGC GCAGTGAAGG ACTACGCCCT CTTCACGTGT 
ACGGCCACGA ACACCCTGGG GGTCGACAGT TTCACCATTC AGCTTGTCAG CACCAGCCGT 
CCTGACCCTC CTTCAGGTCT GAGAGTCATC GGTTTCACTC ACAACTCGGT GACGCTACAA 
TGGAGTGCTG GATTTGATGG AGGGGCGGAG CAAAAATTTC GAGTCAGGTA CCGCTGGCCC 
GGCACCGACA GCCACATGTA TGTAGATGTA TTCCCACCCC AGGAAACCGT ATTCACCATC 
ACAGGCCTGA AAGGAAGCAC CGCCTACAAC TTCTCAGTCA ATGCTATCAA TGCCTTGGGG 
GAAAGCGACT ACGCAGACCA GGGGGCGGTG GTTAGTGTCA CCACAAAAGA GACGACGTTG 
CTTCCGGTAC CTACTCAGCA GCCAACCCGT GCACCCACAG CAGAAGCCCT TGAGTGGCCA 
CCATACATAT TAGCAGCTGT GTGTGCCGGA GGTGCCCTCC TTCTGGTCTC CAATGCGGCT 
TTTATTGGGT GCCTCGTGAA AAGAAGCAGA GACAAGTCCC AAGGAGGAGA AGGTGTGAAG 
AAGGATGAGA GCCAGCAACG CGCACTTAAT GAATACGGAG ACGGGGAGCT CATCAACACC 
CACGCCAAGA AGACTCTACT GATTGACAGC GGTTCCGAGA CAGGTTCTAG TCTTTACCAA 
GACAGCGCTT CAGAATCTGG AATATATTAC TACCCAGTTA GAGATTACAG ACCTTCGCTT 
TCCCCTCAAT ATGAGGTTCC AGAGAGAGGA GAATCAGGGA TCCGAGGTTG GGAAGAGGGC 
GGCTATGAAG ACTGGTATTC GACTTCCGAA GGCCACGAGT ATGAAGAAGT AGGAGATTAC 
CCACAATCCT CTAGAGTCCG ATGGCAGAAC AGAGAAACGG CCAATCAGGG AAGAGAGGCA 
TCGTGGGCAG AATACCAGGA TCCAGGCAGA TTAAGAATCT ATGACGAGGC AGACGTGACC 
TTGTCTGACG TGTACGAGAC TCCGTATGGG GGGCGGGGGC AACTAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761552.1
CDS	194..3904
Translation	MLPVLFAACCSWWILREGVTAKQQVFRVQPDNITVVQGSTALLSCEVEHGTGMVQWEKNGLLLGPDRNIPGFLRYSMTGDPHKGEYNLRIERSELDDDAEYHCQVGRSEVSLGIISRTALINVLIPPKAPTFEEYAAGSRVTWVYGVEYTVTCQATDAKPAAALTIKKSGLEVSGDSSSNPGSRDKLENTAMVAKVIPQSSDNGKLLTCLALNPALQTPITVSFTMNVLFPPNAPIIEGYIGPSVKAGETLKLICISLSGNPLATLQWSKNDDVVSTRWETDEVVRASRSHLTLNIKPADNMAVVSCEAVNHVTPETLKTSIILNVVFLPTEVTILGSSSGPEKSNLTFSCFTAASNPPVQIRWWLGPKELVNTIVTISDAVHGGKVTMSNLTMEALREEHGMALTCEAFNEAILYTKSNSVTLSVQYPPQNVWIEAPPADKFFRAGTSVKMTCFSSGGNPPPRLTWIKDTKSLSGGSQIHSGKIVTKEITIITVPSDNMATYRCNASNIGKTPALTASTKLKVQFPAIDVNIISSAKEYRRGSTIILTCVTGSSNPASTISWVKDGEQLKAQDLGRKPSLYGGISSSSRVTLKAASKDNGRRVSCEAFSSVLNEAVNTFYKLNILFPPEFLDDQPAVVQAVEHGAALIPVRVRANPPQINYTWSLWGEKLIRDGAYRHHLRGEGALEIWNVSRADSGVYNVTCVNKEGKNSIVIRLDVQYSPTIRSLGDTEVDLGSDAEIACTADANPATDSMFQWRWLGDEERDLSALERKVDGVIGRLVIREAKRTDAGRYECTVDNGIPPSIKADARLIVRFKPDIHKGVHLSKVAVPGDGKSVAALVCKAEGIPSVAFSWAKNGVSLDLKDPRYSEKTFHELSVHTSTLVISNVSAVKDYALFTCTATNTLGVDSFTIQLVSTSRPDPPSGLRVIGFTHNSVTLQWSAGFDGGAEQKFRVRYRWPGTDSHMYVDVFPPQETVFTITGLKGSTAYNFSVNAINALGESDYADQGAVVSVTTKETTLLPVPTQQPTRAPTAEALEWPPYILAAVCAGGALLLVSNAAFIGCLVKRSRDKSQGGEGVKKDESQQRALNEYGDGELINTHAKKTLLIDSGSETGSSLYQDSASESGIYYYPVRDYRPSLSPQYEVPERGESGIRGWEEGGYEDWYSTSEGHEYEEVGDYPQSSRVRWQNRETANQGREASWAEYQDPGRLRIYDEADVTLSDVYETPYGGRGQLV

Target ORF information:

RefSeq Version	XM_031905692.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NPHS1 nephrin (nphs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905692.1

ORF Insert Sequence:

ATGCTGCCTG TACTGTTTGC CGCTTGTTGC AGTTGGTGGA TCTTAAGGGA AGGCGTGACG 
GCTAAGCAGC AGGTGTTCCG AGTGCAGCCG GACAATATCA CCGTCGTGCA GGGATCGACC 
GCACTGCTGA GTTGTGAGGT GGAACATGGG ACTGGTATGG TGCAGTGGGA AAAGAATGGA 
CTCCTGCTGG GACCTGACAG GAATATCCCA GGGTTCCTGC GCTACAGCAT GACTGGAGAC 
CCACACAAAG GAGAGTACAA CCTGCGCATT GAGCGTAGCG AGCTGGATGA CGACGCCGAG 
TATCACTGTC AGGTGGGGAG ATCAGAGGTC AGCTTGGGCA TCATTTCCCG CACTGCTCTC 
ATCAACGTCC TCATTCCTCC CAAAGCACCC ACTTTTGAAG AGTATGCTGC GGGCAGCCGA 
GTGACTTGGG TTTACGGGGT GGAATATACA GTCACCTGCC AAGCAACTGA CGCCAAACCG 
GCTGCTGCCT TGACCATCAA AAAGAGTGGA CTGGAGGTGT CTGGAGATTC ATCTTCAAAC 
CCAGGATCCA GAGACAAATT GGAGAACACA GCAATGGTGG CAAAGGTCAT CCCCCAGAGC 
TCTGATAATG GGAAACTGCT GACCTGCTTG GCGCTAAATC CAGCTCTTCA GACGCCAATC 
ACCGTGAGCT TCACCATGAA TGTGCTGTTT CCGCCTAATG CCCCGATTAT AGAAGGTTAT 
ATTGGGCCAT CGGTTAAAGC TGGGGAAACA CTGAAGCTCA TCTGCATTTC TTTAAGTGGA 
AACCCCCTGG CCACATTACA ATGGTCAAAG AACGATGATG TTGTGTCAAC ACGGTGGGAG 
ACGGATGAGG TGGTCCGAGC TTCTCGTAGT CACCTGACCC TGAATATTAA GCCTGCGGAT 
AACATGGCGG TTGTGAGCTG TGAGGCTGTA AATCATGTGA CTCCAGAGAC TCTCAAAACC 
AGCATCATCC TCAATGTTGT GTTTCTGCCA ACAGAAGTAA CCATCCTTGG CTCATCAAGT 
GGCCCAGAGA AATCTAATCT AACGTTCTCC TGCTTTACGG CTGCCAGTAA CCCTCCTGTA 
CAGATCCGCT GGTGGCTGGG CCCAAAGGAA CTAGTGAACA CCATTGTCAC CATCTCTGAT 
GCTGTTCATG GTGGAAAGGT GACAATGTCC AACCTCACTA TGGAGGCTCT TCGAGAGGAG 
CATGGAATGG CGTTGACCTG TGAGGCCTTT AATGAGGCCA TTCTCTACAC CAAATCCAAC 
AGCGTTACCC TAAGTGTGCA ATACCCTCCA CAGAATGTCT GGATCGAGGC CCCTCCAGCT 
GATAAGTTCT TCCGTGCGGG GACATCAGTG AAGATGACTT GTTTCTCTAG CGGTGGAAAC 
CCGCCACCCA GGCTTACCTG GATAAAGGAT ACTAAAAGCC TGAGTGGGGG ATCGCAGATC 
CATTCAGGAA AAATTGTCAC AAAAGAAATT ACAATTATAA CTGTGCCAAG TGACAATATG 
GCCACATACC GGTGCAACGC CAGCAACATT GGGAAGACGC CGGCGCTGAC TGCCAGCACG 
AAACTAAAGG TCCAGTTTCC TGCCATAGAT GTGAATATAA TTAGCAGCGC CAAAGAGTAT 
CGCCGCGGTT CTACCATTAT ACTGACCTGT GTCACCGGCA GCAGTAACCC TGCATCTACT 
ATATCCTGGG TGAAAGATGG AGAGCAGTTA AAAGCTCAGG ATTTGGGAAG AAAACCATCT 
CTGTATGGGG GAATTTCCAG TTCCAGTCGT GTCACACTGA AAGCAGCATC TAAGGACAAC 
GGAAGGCGCG TCTCCTGCGA GGCGTTCAGC AGCGTCCTCA ACGAGGCCGT CAACACCTTC 
TACAAACTCA ATATCCTGTT TCCCCCTGAG TTTCTGGATG ATCAGCCGGC GGTGGTGCAG 
GCCGTGGAGC ATGGAGCTGC CCTTATCCCA GTCCGGGTCA GGGCCAACCC ACCTCAGATC 
AACTACACGT GGAGCCTTTG GGGCGAGAAA CTGATCCGAG ATGGTGCTTA CCGACATCAC 
CTGCGCGGTG AGGGGGCCCT CGAGATATGG AACGTGAGTC GCGCTGACTC CGGGGTCTAC 
AATGTGACTT GTGTGAATAA AGAGGGGAAG AACAGCATTG TAATAAGACT GGATGTGCAG 
TATTCTCCCA CCATTCGAAG CCTTGGGGAC ACAGAGGTGG ATCTCGGTTC AGACGCAGAG 
ATCGCTTGCA CGGCAGATGC GAATCCAGCG ACAGACTCAA TGTTCCAGTG GAGATGGCTG 
GGTGATGAGG AGCGAGACCT CTCTGCCTTG GAACGCAAAG TGGATGGAGT CATTGGACGC 
CTGGTTATCC GGGAAGCCAA GAGAACGGAC GCCGGCCGCT ACGAGTGCAC CGTGGATAAC 
GGGATCCCCC CCTCCATCAA GGCTGATGCT CGTCTGATTG TCCGATTTAA GCCTGATATA 
CACAAGGGGG TGCACTTGAG CAAAGTGGCA GTTCCTGGTG ATGGGAAGAG CGTGGCAGCC 
TTGGTGTGTA AAGCTGAGGG AATCCCGAGT GTGGCGTTTA GCTGGGCTAA GAATGGGGTA 
TCTCTGGATC TCAAAGATCC CAGGTACTCA GAGAAGACAT TTCATGAGCT GTCAGTTCAC 
ACCAGCACGC TGGTCATTTC CAATGTGAGC GCAGTGAAGG ACTACGCCCT CTTCACGTGT 
ACGGCCACGA ACACCCTGGG GGTCGACAGT TTCACCATTC AGCTTGTCAG CACCAGCCGT 
CCTGACCCTC CTTCAGGTCT GAGAGTCATC GGTTTCACTC ACAACTCGGT GACGCTACAA 
TGGAGTGCTG GATTTGATGG AGGGGCGGAG CAAAAATTTC GAGTCAGGTA CCGCTGGCCC 
GGCACCGACA GCCACATGTA TGTAGATGTA TTCCCACCCC AGGAAACCGT ATTCACCATC 
ACAGGCCTGA AAGGAAGCAC CGCCTACAAC TTCTCAGTCA ATGCTATCAA TGCCTTGGGG 
GAAAGCGACT ACGCAGACCA GGGGGCGGTG GTTAGTGTCA CCACAAAAGA GACGACGTTG 
CTTCCGGTAC CTACTCAGCA GCCAACCCGT GCACCCACAG CAGAAGCCCT TGAGTGGCCA 
CCATACATAT TAGCAGCTGT GTGTGCCGGA GGTGCCCTCC TTCTGGTCTC CAATGCGGCT 
TTTATTGGGT GCCTCGTGAA AAGAAGCAGA GACAAGTCCC AAGGAGGAGA AGGTGTGAAG 
AAGGATGAGA GCCAGCAACG CGCACTTAAT GAATACGGAG ACGGGGAGCT CATCAACACC 
CACGCCAAGA AGACTCTACT GATTGACAGC GGTTCCGAGA CAGGTTCTAG TCTTTACCAA 
GACAGCGCTT CAGAATCTGG AATATATTAC TACCCAGTTA GAGATTACAG ACCTTCGCTT 
TCCCCTCAAT ATGAGGTTCC AGAGAGAGGA GAATCAGGGA TCCGAGGTTG GGAAGAGGGC 
GGCTATGAAG ACTGGTATTC GACTTCCGAA GGCCACGAGT ATGAAGAAGT AGGAGATTAC 
CCACAATCCT CTAGAGTCCG ATGGCAGAAC AGAGAAACGG CCAATCAGGG AAGAGAGGCA 
TCGTGGGCAG AATACCAGGA TCCAGGCAGA TTAAGAATCT ATGACGAGGC AGACGTGACC 
TTGTCTGACG TGTACGAGAC TCCGTATGGG GGGCGGGGGC AACTAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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nphs1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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