si:ch211-234p18.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:ch211-234p18.3
Entrez Gene ID	568885
Full Name	si:ch211-234p18.3
Synonyms	rasgrf1
General protein information	Preferred Names si:ch211-234p18.3 Names ras-specific guanine nucleotide-releasing factor 1 Ras protein-specific guanine nucleotide-releasing factor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002665349.4	XP_002665395.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_009293629.3	XP_009291904.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_009293629.2	XP_009291904.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_002665349.5	XP_002665395.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	RASGRF1	NP_002882.3
Mus musculus (house mouse)	Rasgrf1	NP_035375.1
Danio rerio (zebrafish)	si:ch211-234p18.3	XP_002665395.1
Xenopus tropicalis (tropical clawed frog)	rasgrf1	XP_002933009.2
Gallus gallus (chicken)	RASGRF1	XP_004944008.1
Pan troglodytes (chimpanzee)	RASGRF1	XP_001153586.1
Canis lupus familiaris (dog)	RASGRF1	XP_545892.5
Rattus norvegicus (Norway rat)	Rasgrf1	NP_001164002.1
Bos taurus (cattle)	RASGRF1	NP_001178386.1

Gene Ontology

The following si:ch211-234p18.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-234p18.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23369	XM_002665349.5 Latest version!	Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45587	XM_009293629.3 Latest version!	Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa45587	XM_009293629.2	Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa23369	XM_002665349.4	Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:ch211-234p18.3 ( XM_002665349.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002665395.1 Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23369
Clone ID Related Accession (Same CDS sequence)	XM_002665349.4 , XM_002665349.5
Accession Version	XM_002665349.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002665349.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GAATTCGGCT GAATGATGGT CATGTCACCT ACCTGGGTCT TTTGGCGAAG 
AAGGATGGCA CGAGGCGAGG CTGCTTAAGC AAAAAGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TTGCTCTGTT GCAAAATATG CTTTTTTATT TTGAGAGCGA GTCCAGCTCG 
CGACCCTCGG GATTATACCT GTTGGAGGGA TGTGTGTGTG ACAGGTCGCC CTCACCCAAA 
CCTTCACTGT CAGCCAAGGA GTGCTTAGAA AAACAGTATT ATTTCACAGT CAGCTTCAAC 
CATGAGAATC AGAAGGCCCT GGAGCTTCGC ACAGAAGATG TTAAGGACTG CGATGAATGG 
GTGGCAGCAA TATCACATGC CAGCTACAGA AACCTGGCCA CCGAGCATGA AACGCTCATG 
CAGAAGTACC TCCATCTACT CCAAATCGTG GAGACCGAGA AAACAGTTGC TAAGCAACTT 
CGACAACAGA TAGAAGATGG GGAGATTGAG ATTGAACGTC TAAAATCAGA GATTGCAGCA 
ATGCTCAAAG ACAATGAAAA GATTCAGTCA AGTCCCACCA CAGCACCCAG CGATGATGAT 
TCGGAGATCA AGAAAATCAA GAAAGTGCAA AGCTTCCTGA GAGGCTGGAT GTGTCGGCGG 
AAGTGGAAGA CCATCATTCA GGACTACATC CGTTCTCCAC ATGCCGAGAG CATGAGGAAG 
AGAAACCAGG TGGTCTTTAG CATGCTGGAG GCGGAGGCTG AGTACGTCCA GCAGCTCCAC 
ATCCTTGTCA ACAACTTCTT GAGACCGCTG CGCATGGCTG CCAGCTCCAA AAAGCCACCC 
ATAACCCACG ATGATGTCAG CAGCATATTC CTCAACAGTG AAACCATAAT GTTTCTGCAT 
CAGATATTCT ACCAAGGGTT GAAAGCTCGA ATAGCAAGCT GGCCAACATT AGTCTTGGCT 
GACTTGTTTG ACATCCTGTT GCCCATGCTG AACATCTACC AGGAGTTTGT GCGTAATCAC 
CAGTACAGCT TGCAGATCTT GGCCCACTGC AAGCAGAACC GCGATTTCGA CAAGCTGCTG 
AAACAGTATG AAGCCAAGCC TGACTGTGAG GAACGCACGC TGGAGACGTT CCTTACTTAT 
CCCATGTTTC AGATTCCTCG TTACATACTG ACCCTCCATG AGCTGTTAGC ACATACACCG 
CATGAGCATG TGGAGAGAAA CAGCTTGGAT TACGCCAAGT CGAAGTTGGA GGAGCTTTCC 
AGAATCATGC ATGATGAGGT GAGCGAGACG GAGAACATCC GGAAGAACCT CGCTATCGAG 
CGCATGATCG TGGAGGGCTG TGAGATTCTC CTAGACACCA GCCAGACCTT TGTCAGACAA 
GGATCTCTGA TTCAAGTGCC CATGAGTGAG AAGGGCAAGA TCACCCGGGG GCGACTGGGG 
TCTCTGTCTC TAAAGAAAGA GGGTGAGAGG CAGTGTTTCC TCTTCTCCAA ACATCTAATC 
ATCTGCACTA GAGGCTCTGG AGGGAAACTG CACCTCACCA AGAACGGAGT GGTGTCGCTC 
ATTGACTGCA CACTGATAGA GGAACCAGAG GGCACTGATG ACGAGTCCAA ATCAGAGAAG 
AGCGGTCAGG ACATGGAGCA CCTGGATTTT AAGATTGTGG TGGAGCCCAA AGACAGCCAG 
TCATTCACCT TCATCCTGGT GGCCTCCTCG CGGCAAGAGA AGTCAGCCTG GACCAGTGAC 
ATCAGCCAGT GCATAGATAA TATTCGCTGT AATGGGCTGA TGATGAACGC CTTTGAGGAG 
AACTCTAAAG TCACTGTGCC ACAGATGATC AAGTCTGATG CCAGTCTGTA CTGTGATGAC 
GTAGACATCC GCTTCAGTAA GATGATGAAC TCGTGCAAGG TGTTGCAGAT CCGCTATGCC 
AGTGTGGAGC GTCTGCTGGA GAGGCTGACC GACCTGCGCT TCCTCTCCAT TGACTTTCTC 
AACACCTTCC TGCACTCCTA CCGCGTGTTC ACCAGTGCTG ACGTCGTTCT GGACAAGCTC 
ATCACCATTT ACAAAAGGCC CATCAGCGCC ATACCAGCCC GATCCCTGGA GCTGTTTTTT 
GCGAGCAGCC AGAACAACAA GCTCCTCTAT GGAGAGCCTC CCAAGTCGCC CCGTGCCAGC 
CGCAAGTTTT CGTCCCCACC GCCACTGGCC ATCACTAAGA CTTCATCTCC CAATCGCCGC 
CGCAAACTGT CCCTCAACAT CCCCATCATC ACCGGAGGGA AGGCTCTGGA TCTGGCAGCC 
CTCAGCTGTT CCTCCAATGG CTATGCAAGC ATGTACTCTA CCATGTCCCC CTTCAGCAAG 
ACCACTCTGG ACATCAACAA GCTCTATGTG TCCAGTCCCA TCACCAGCAA GATCCCCGAC 
GAGGGAGAGG GCAAGACGGA CAAGGCAGAT GAAATTTCTC TCTGCAAACA AGACAATTCA 
GTGAGGGAGG AAAGCGATAA CGACCAGATC CAGAGTGATG AGGCAGAGGC TGAAGTGTCG 
CCCACCAAAT CACCCACCAC TCCCAAAAAC GTCAAATGCA AAAACTCCTC AGAATTCTCT 
CTGTTCTCTT ACAATAATGG CATGGTGATG TCATCCTGCC GGGAGCTGGA TAACAACCGT 
AGCGCCTTGT CTGCCGCTTC TGCCTTCGCC ATCGCCACTG CAGGTGCCAA CGAGGGCACG 
CCAACCAAGG AGAAGTACCG CCGCATGTCT TTGGCAAGCA CAGGGTTCCC TACGGATCAG 
AGGAACGGAG ATAAGGAGTT TGTGATTCGC CGAGCAGCAA CTAACAGAGT CCTCAATGTC 
CTCAGGCACT GGGTGTCCAA ACACTCACAG GACTTTGAAA CCAACACTGA GCTCAAGATG 
AAGGTAATCA GCTTCCTGGA GGAGGTGATG CACGATCCTG AACTTCTGAC CCAAGAAAGA 
AAGGCTGCTG CCAACATTAT ACGAACACTA ACGCAAGAGG ATCCTGGTGA TAATCAGATC 
TGTCTGGAGG AGGTTCTGCA GATGGCTGAA GGAGGAAAGT CAGAGTCTTT CGAGAACCAT 
TCAGCTCTGG AGATTGCAGA GCAGCTCACC CTACTTGACC ACCTTGTATT CAAAGTCATA 
CCATATGAAG AGTTTTTTGG TCAGGGATGG ATGAAGAATG ACAAAAATGA AAAGACACCT 
TACATCATGA AAACAACAAA ACACTTCAAC GATATCAGTG ACCTGATCGC TACAGAGATC 
CTGCGCTGTG AGGATGTGAA TGTACGGGTA GCAGTGATGG AGAAGTGGGT GGCGGTGGCT 
GATATCTGCC GCTGTCTCCA CAACTACAAT GCTGTGCTGG AAATCACCTC GTCCCTCAAT 
CGCAGCTCCA TCTTCCGCCT GAAGAAAACC TGGCTCAAAG TCTCCAAGCA GACCAAAACA 
GTCATTGACA AGCTGCAGAA GTTGGTCTCG TCTGAAGGAA GGTTCAAGAA CCTCAGAGAG 
GCCTTAAAAA ATTGTGACCC TCCCTGTGTG CCATATTTGG GAATGTATCT CACTGACTTG 
GCCTTTATTG AGGAGGGGAC GCCCAACTAC ACAGAGGACA ACCTGGTCAA TTTTTCCAAG 
ATGCGAATGA TTTCCCATAT CATCCGAGAA ATCAGGCAGT TTCAGCAAAC GGCGTATAAG 
ATTGATTACC AGCCGAAGGC AGCACTATAT TTACTGGACA GAAGCGCAGT AATGGAAGAA 
GAAGGCCTGT ATGAAGCCTC TCTCAGAATT GAGCCTAAAG TGCCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665395.1
CDS	381..4151
Translation	MQKGIRLNDGHVTYLGLLAKKDGTRRGCLSKKSSDNTKWHTKWFALLQNMLFYFESESSSRPSGLYLLEGCVCDRSPSPKPSLSAKECLEKQYYFTVSFNHENQKALELRTEDVKDCDEWVAAISHASYRNLATEHETLMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKSEIAAMLKDNEKIQSSPTTAPSDDDSEIKKIKKVQSFLRGWMCRRKWKTIIQDYIRSPHAESMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIVEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVVSLIDCTLIEEPEGTDDESKSEKSGQDMEHLDFKIVVEPKDSQSFTFILVASSRQEKSAWTSDISQCIDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKMMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTSADVVLDKLITIYKRPISAIPARSLELFFASSQNNKLLYGEPPKSPRASRKFSSPPPLAITKTSSPNRRRKLSLNIPIITGGKALDLAALSCSSNGYASMYSTMSPFSKTTLDINKLYVSSPITSKIPDEGEGKTDKADEISLCKQDNSVREESDNDQIQSDEAEAEVSPTKSPTTPKNVKCKNSSEFSLFSYNNGMVMSSCRELDNNRSALSAASAFAIATAGANEGTPTKEKYRRMSLASTGFPTDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNTELKMKVISFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQICLEEVLQMAEGGKSESFENHSALEIAEQLTLLDHLVFKVIPYEEFFGQGWMKNDKNEKTPYIMKTTKHFNDISDLIATEILRCEDVNVRVAVMEKWVAVADICRCLHNYNAVLEITSSLNRSSIFRLKKTWLKVSKQTKTVIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDNLVNFSKMRMISHIIREIRQFQQTAYKIDYQPKAALYLLDRSAVMEEEGLYEASLRIEPKVPN

Target ORF information:

RefSeq Version	XM_002665349.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665349.4

ORF Insert Sequence:

ATGCAGAAGG GAATTCGGCT GAATGATGGT CATGTCACCT ACCTGGGTCT TTTGGCGAAG 
AAGGATGGCA CGAGGCGAGG CTGCTTAAGC AAAAAGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TTGCTCTGTT GCAAAATATG CTTTTTTATT TTGAGAGCGA GTCCAGCTCG 
CGACCCTCGG GATTATACCT GTTGGAGGGA TGTGTGTGTG ACAGGTCGCC CTCACCCAAA 
CCTTCACTGT CAGCCAAGGA GTGCTTAGAA AAACAGTATT ATTTCACAGT CAGCTTCAAC 
CATGAGAATC AGAAGGCCCT GGAGCTTCGC ACAGAAGATG TTAAGGACTG CGATGAATGG 
GTGGCAGCAA TATCACATGC CAGCTACAGA AACCTGGCCA CCGAGCATGA AACGCTCATG 
CAGAAGTACC TCCATCTACT CCAAATCGTG GAGACCGAGA AAACAGTTGC TAAGCAACTT 
CGACAACAGA TAGAAGATGG GGAGATTGAG ATTGAACGTC TAAAATCAGA GATTGCAGCA 
ATGCTCAAAG ACAATGAAAA GATTCAGTCA AGTCCCACCA CAGCACCCAG CGATGATGAT 
TCGGAGATCA AGAAAATCAA GAAAGTGCAA AGCTTCCTGA GAGGCTGGAT GTGTCGGCGG 
AAGTGGAAGA CCATCATTCA GGACTACATC CGTTCTCCAC ATGCCGAGAG CATGAGGAAG 
AGAAACCAGG TGGTCTTTAG CATGCTGGAG GCGGAGGCTG AGTACGTCCA GCAGCTCCAC 
ATCCTTGTCA ACAACTTCTT GAGACCGCTG CGCATGGCTG CCAGCTCCAA AAAGCCACCC 
ATAACCCACG ATGATGTCAG CAGCATATTC CTCAACAGTG AAACCATAAT GTTTCTGCAT 
CAGATATTCT ACCAAGGGTT GAAAGCTCGA ATAGCAAGCT GGCCAACATT AGTCTTGGCT 
GACTTGTTTG ACATCCTGTT GCCCATGCTG AACATCTACC AGGAGTTTGT GCGTAATCAC 
CAGTACAGCT TGCAGATCTT GGCCCACTGC AAGCAGAACC GCGATTTCGA CAAGCTGCTG 
AAACAGTATG AAGCCAAGCC TGACTGTGAG GAACGCACGC TGGAGACGTT CCTTACTTAT 
CCCATGTTTC AGATTCCTCG TTACATACTG ACCCTCCATG AGCTGTTAGC ACATACACCG 
CATGAGCATG TGGAGAGAAA CAGCTTGGAT TACGCCAAGT CGAAGTTGGA GGAGCTTTCC 
AGAATCATGC ATGATGAGGT GAGCGAGACG GAGAACATCC GGAAGAACCT CGCTATCGAG 
CGCATGATCG TGGAGGGCTG TGAGATTCTC CTAGACACCA GCCAGACCTT TGTCAGACAA 
GGATCTCTGA TTCAAGTGCC CATGAGTGAG AAGGGCAAGA TCACCCGGGG GCGACTGGGG 
TCTCTGTCTC TAAAGAAAGA GGGTGAGAGG CAGTGTTTCC TCTTCTCCAA ACATCTAATC 
ATCTGCACTA GAGGCTCTGG AGGGAAACTG CACCTCACCA AGAACGGAGT GGTGTCGCTC 
ATTGACTGCA CACTGATAGA GGAACCAGAG GGCACTGATG ACGAGTCCAA ATCAGAGAAG 
AGCGGTCAGG ACATGGAGCA CCTGGATTTT AAGATTGTGG TGGAGCCCAA AGACAGCCAG 
TCATTCACCT TCATCCTGGT GGCCTCCTCG CGGCAAGAGA AGTCAGCCTG GACCAGTGAC 
ATCAGCCAGT GCATAGATAA TATTCGCTGT AATGGGCTGA TGATGAACGC CTTTGAGGAG 
AACTCTAAAG TCACTGTGCC ACAGATGATC AAGTCTGATG CCAGTCTGTA CTGTGATGAC 
GTAGACATCC GCTTCAGTAA GATGATGAAC TCGTGCAAGG TGTTGCAGAT CCGCTATGCC 
AGTGTGGAGC GTCTGCTGGA GAGGCTGACC GACCTGCGCT TCCTCTCCAT TGACTTTCTC 
AACACCTTCC TGCACTCCTA CCGCGTGTTC ACCAGTGCTG ACGTCGTTCT GGACAAGCTC 
ATCACCATTT ACAAAAGGCC CATCAGCGCC ATACCAGCCC GATCCCTGGA GCTGTTTTTT 
GCGAGCAGCC AGAACAACAA GCTCCTCTAT GGAGAGCCTC CCAAGTCGCC CCGTGCCAGC 
CGCAAGTTTT CGTCCCCACC GCCACTGGCC ATCACTAAGA CTTCATCTCC CAATCGCCGC 
CGCAAACTGT CCCTCAACAT CCCCATCATC ACCGGAGGGA AGGCTCTGGA TCTGGCAGCC 
CTCAGCTGTT CCTCCAATGG CTATGCAAGC ATGTACTCTA CCATGTCCCC CTTCAGCAAG 
ACCACTCTGG ACATCAACAA GCTCTATGTG TCCAGTCCCA TCACCAGCAA GATCCCCGAC 
GAGGGAGAGG GCAAGACGGA CAAGGCAGAT GAAATTTCTC TCTGCAAACA AGACAATTCA 
GTGAGGGAGG AAAGCGATAA CGACCAGATC CAGAGTGATG AGGCAGAGGC TGAAGTGTCG 
CCCACCAAAT CACCCACCAC TCCCAAAAAC GTCAAATGCA AAAACTCCTC AGAATTCTCT 
CTGTTCTCTT ACAATAATGG CATGGTGATG TCATCCTGCC GGGAGCTGGA TAACAACCGT 
AGCGCCTTGT CTGCCGCTTC TGCCTTCGCC ATCGCCACTG CAGGTGCCAA CGAGGGCACG 
CCAACCAAGG AGAAGTACCG CCGCATGTCT TTGGCAAGCA CAGGGTTCCC TACGGATCAG 
AGGAACGGAG ATAAGGAGTT TGTGATTCGC CGAGCAGCAA CTAACAGAGT CCTCAATGTC 
CTCAGGCACT GGGTGTCCAA ACACTCACAG GACTTTGAAA CCAACACTGA GCTCAAGATG 
AAGGTAATCA GCTTCCTGGA GGAGGTGATG CACGATCCTG AACTTCTGAC CCAAGAAAGA 
AAGGCTGCTG CCAACATTAT ACGAACACTA ACGCAAGAGG ATCCTGGTGA TAATCAGATC 
TGTCTGGAGG AGGTTCTGCA GATGGCTGAA GGAGGAAAGT CAGAGTCTTT CGAGAACCAT 
TCAGCTCTGG AGATTGCAGA GCAGCTCACC CTACTTGACC ACCTTGTATT CAAAGTCATA 
CCATATGAAG AGTTTTTTGG TCAGGGATGG ATGAAGAATG ACAAAAATGA AAAGACACCT 
TACATCATGA AAACAACAAA ACACTTCAAC GATATCAGTG ACCTGATCGC TACAGAGATC 
CTGCGCTGTG AGGATGTGAA TGTACGGGTA GCAGTGATGG AGAAGTGGGT GGCGGTGGCT 
GATATCTGCC GCTGTCTCCA CAACTACAAT GCTGTGCTGG AAATCACCTC GTCCCTCAAT 
CGCAGCTCCA TCTTCCGCCT GAAGAAAACC TGGCTCAAAG TCTCCAAGCA GACCAAAACA 
GTCATTGACA AGCTGCAGAA GTTGGTCTCG TCTGAAGGAA GGTTCAAGAA CCTCAGAGAG 
GCCTTAAAAA ATTGTGACCC TCCCTGTGTG CCATATTTGG GAATGTATCT CACTGACTTG 
GCCTTTATTG AGGAGGGGAC GCCCAACTAC ACAGAGGACA ACCTGGTCAA TTTTTCCAAG 
ATGCGAATGA TTTCCCATAT CATCCGAGAA ATCAGGCAGT TTCAGCAAAC GGCGTATAAG 
ATTGATTACC AGCCGAAGGC AGCACTATAT TTACTGGACA GAAGCGCAGT AATGGAAGAA 
GAAGGCCTGT ATGAAGCCTC TCTCAGAATT GAGCCTAAAG TGCCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-234p18.3 ( XM_002665349.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002665395.1 Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23369
Clone ID Related Accession (Same CDS sequence)	XM_002665349.4 , XM_002665349.5
Accession Version	XM_002665349.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002665349.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GAATTCGGCT GAATGATGGT CATGTCACCT ACCTGGGTCT TTTGGCGAAG 
AAGGATGGCA CGAGGCGAGG CTGCTTAAGC AAAAAGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TTGCTCTGTT GCAAAATATG CTTTTTTATT TTGAGAGCGA GTCCAGCTCG 
CGACCCTCGG GATTATACCT GTTGGAGGGA TGTGTGTGTG ACAGGTCGCC CTCACCCAAA 
CCTTCACTGT CAGCCAAGGA GTGCTTAGAA AAACAGTATT ATTTCACAGT CAGCTTCAAC 
CATGAGAATC AGAAGGCCCT GGAGCTTCGC ACAGAAGATG TTAAGGACTG CGATGAATGG 
GTGGCAGCAA TATCACATGC CAGCTACAGA AACCTGGCCA CCGAGCATGA AACGCTCATG 
CAGAAGTACC TCCATCTACT CCAAATCGTG GAGACCGAGA AAACAGTTGC TAAGCAACTT 
CGACAACAGA TAGAAGATGG GGAGATTGAG ATTGAACGTC TAAAATCAGA GATTGCAGCA 
ATGCTCAAAG ACAATGAAAA GATTCAGTCA AGTCCCACCA CAGCACCCAG CGATGATGAT 
TCGGAGATCA AGAAAATCAA GAAAGTGCAA AGCTTCCTGA GAGGCTGGAT GTGTCGGCGG 
AAGTGGAAGA CCATCATTCA GGACTACATC CGTTCTCCAC ATGCCGAGAG CATGAGGAAG 
AGAAACCAGG TGGTCTTTAG CATGCTGGAG GCGGAGGCTG AGTACGTCCA GCAGCTCCAC 
ATCCTTGTCA ACAACTTCTT GAGACCGCTG CGCATGGCTG CCAGCTCCAA AAAGCCACCC 
ATAACCCACG ATGATGTCAG CAGCATATTC CTCAACAGTG AAACCATAAT GTTTCTGCAT 
CAGATATTCT ACCAAGGGTT GAAAGCTCGA ATAGCAAGCT GGCCAACATT AGTCTTGGCT 
GACTTGTTTG ACATCCTGTT GCCCATGCTG AACATCTACC AGGAGTTTGT GCGTAATCAC 
CAGTACAGCT TGCAGATCTT GGCCCACTGC AAGCAGAACC GCGATTTCGA CAAGCTGCTG 
AAACAGTATG AAGCCAAGCC TGACTGTGAG GAACGCACGC TGGAGACGTT CCTTACTTAT 
CCCATGTTTC AGATTCCTCG TTACATACTG ACCCTCCATG AGCTGTTAGC ACATACACCG 
CATGAGCATG TGGAGAGAAA CAGCTTGGAT TACGCCAAGT CGAAGTTGGA GGAGCTTTCC 
AGAATCATGC ATGATGAGGT GAGCGAGACG GAGAACATCC GGAAGAACCT CGCTATCGAG 
CGCATGATCG TGGAGGGCTG TGAGATTCTC CTAGACACCA GCCAGACCTT TGTCAGACAA 
GGATCTCTGA TTCAAGTGCC CATGAGTGAG AAGGGCAAGA TCACCCGGGG GCGACTGGGG 
TCTCTGTCTC TAAAGAAAGA GGGTGAGAGG CAGTGTTTCC TCTTCTCCAA ACATCTAATC 
ATCTGCACTA GAGGCTCTGG AGGGAAACTG CACCTCACCA AGAACGGAGT GGTGTCGCTC 
ATTGACTGCA CACTGATAGA GGAACCAGAG GGCACTGATG ACGAGTCCAA ATCAGAGAAG 
AGCGGTCAGG ACATGGAGCA CCTGGATTTT AAGATTGTGG TGGAGCCCAA AGACAGCCAG 
TCATTCACCT TCATCCTGGT GGCCTCCTCG CGGCAAGAGA AGTCAGCCTG GACCAGTGAC 
ATCAGCCAGT GCATAGATAA TATTCGCTGT AATGGGCTGA TGATGAACGC CTTTGAGGAG 
AACTCTAAAG TCACTGTGCC ACAGATGATC AAGTCTGATG CCAGTCTGTA CTGTGATGAC 
GTAGACATCC GCTTCAGTAA GATGATGAAC TCGTGCAAGG TGTTGCAGAT CCGCTATGCC 
AGTGTGGAGC GTCTGCTGGA GAGGCTGACC GACCTGCGCT TCCTCTCCAT TGACTTTCTC 
AACACCTTCC TGCACTCCTA CCGCGTGTTC ACCAGTGCTG ACGTCGTTCT GGACAAGCTC 
ATCACCATTT ACAAAAGGCC CATCAGCGCC ATACCAGCCC GATCCCTGGA GCTGTTTTTT 
GCGAGCAGCC AGAACAACAA GCTCCTCTAT GGAGAGCCTC CCAAGTCGCC CCGTGCCAGC 
CGCAAGTTTT CGTCCCCACC GCCACTGGCC ATCACTAAGA CTTCATCTCC CAATCGCCGC 
CGCAAACTGT CCCTCAACAT CCCCATCATC ACCGGAGGGA AGGCTCTGGA TCTGGCAGCC 
CTCAGCTGTT CCTCCAATGG CTATGCAAGC ATGTACTCTA CCATGTCCCC CTTCAGCAAG 
ACCACTCTGG ACATCAACAA GCTCTATGTG TCCAGTCCCA TCACCAGCAA GATCCCCGAC 
GAGGGAGAGG GCAAGACGGA CAAGGCAGAT GAAATTTCTC TCTGCAAACA AGACAATTCA 
GTGAGGGAGG AAAGCGATAA CGACCAGATC CAGAGTGATG AGGCAGAGGC TGAAGTGTCG 
CCCACCAAAT CACCCACCAC TCCCAAAAAC GTCAAATGCA AAAACTCCTC AGAATTCTCT 
CTGTTCTCTT ACAATAATGG CATGGTGATG TCATCCTGCC GGGAGCTGGA TAACAACCGT 
AGCGCCTTGT CTGCCGCTTC TGCCTTCGCC ATCGCCACTG CAGGTGCCAA CGAGGGCACG 
CCAACCAAGG AGAAGTACCG CCGCATGTCT TTGGCAAGCA CAGGGTTCCC TACGGATCAG 
AGGAACGGAG ATAAGGAGTT TGTGATTCGC CGAGCAGCAA CTAACAGAGT CCTCAATGTC 
CTCAGGCACT GGGTGTCCAA ACACTCACAG GACTTTGAAA CCAACACTGA GCTCAAGATG 
AAGGTAATCA GCTTCCTGGA GGAGGTGATG CACGATCCTG AACTTCTGAC CCAAGAAAGA 
AAGGCTGCTG CCAACATTAT ACGAACACTA ACGCAAGAGG ATCCTGGTGA TAATCAGATC 
TGTCTGGAGG AGGTTCTGCA GATGGCTGAA GGAGGAAAGT CAGAGTCTTT CGAGAACCAT 
TCAGCTCTGG AGATTGCAGA GCAGCTCACC CTACTTGACC ACCTTGTATT CAAAGTCATA 
CCATATGAAG AGTTTTTTGG TCAGGGATGG ATGAAGAATG ACAAAAATGA AAAGACACCT 
TACATCATGA AAACAACAAA ACACTTCAAC GATATCAGTG ACCTGATCGC TACAGAGATC 
CTGCGCTGTG AGGATGTGAA TGTACGGGTA GCAGTGATGG AGAAGTGGGT GGCGGTGGCT 
GATATCTGCC GCTGTCTCCA CAACTACAAT GCTGTGCTGG AAATCACCTC GTCCCTCAAT 
CGCAGCTCCA TCTTCCGCCT GAAGAAAACC TGGCTCAAAG TCTCCAAGCA GACCAAAACA 
GTCATTGACA AGCTGCAGAA GTTGGTCTCG TCTGAAGGAA GGTTCAAGAA CCTCAGAGAG 
GCCTTAAAAA ATTGTGACCC TCCCTGTGTG CCATATTTGG GAATGTATCT CACTGACTTG 
GCCTTTATTG AGGAGGGGAC GCCCAACTAC ACAGAGGACA ACCTGGTCAA TTTTTCCAAG 
ATGCGAATGA TTTCCCATAT CATCCGAGAA ATCAGGCAGT TTCAGCAAAC GGCGTATAAG 
ATTGATTACC AGCCGAAGGC AGCACTATAT TTACTGGACA GAAGCGCAGT AATGGAAGAA 
GAAGGCCTGT ATGAAGCCTC TCTCAGAATT GAGCCTAAAG TGCCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665395.1
CDS	1..3771
Translation	MQKGIRLNDGHVTYLGLLAKKDGTRRGCLSKKSSDNTKWHTKWFALLQNMLFYFESESSSRPSGLYLLEGCVCDRSPSPKPSLSAKECLEKQYYFTVSFNHENQKALELRTEDVKDCDEWVAAISHASYRNLATEHETLMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKSEIAAMLKDNEKIQSSPTTAPSDDDSEIKKIKKVQSFLRGWMCRRKWKTIIQDYIRSPHAESMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIVEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVVSLIDCTLIEEPEGTDDESKSEKSGQDMEHLDFKIVVEPKDSQSFTFILVASSRQEKSAWTSDISQCIDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKMMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTSADVVLDKLITIYKRPISAIPARSLELFFASSQNNKLLYGEPPKSPRASRKFSSPPPLAITKTSSPNRRRKLSLNIPIITGGKALDLAALSCSSNGYASMYSTMSPFSKTTLDINKLYVSSPITSKIPDEGEGKTDKADEISLCKQDNSVREESDNDQIQSDEAEAEVSPTKSPTTPKNVKCKNSSEFSLFSYNNGMVMSSCRELDNNRSALSAASAFAIATAGANEGTPTKEKYRRMSLASTGFPTDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNTELKMKVISFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQICLEEVLQMAEGGKSESFENHSALEIAEQLTLLDHLVFKVIPYEEFFGQGWMKNDKNEKTPYIMKTTKHFNDISDLIATEILRCEDVNVRVAVMEKWVAVADICRCLHNYNAVLEITSSLNRSSIFRLKKTWLKVSKQTKTVIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDNLVNFSKMRMISHIIREIRQFQQTAYKIDYQPKAALYLLDRSAVMEEEGLYEASLRIEPKVPN

Target ORF information:

RefSeq Version	XM_002665349.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665349.5

ORF Insert Sequence:

ATGCAGAAGG GAATTCGGCT GAATGATGGT CATGTCACCT ACCTGGGTCT TTTGGCGAAG 
AAGGATGGCA CGAGGCGAGG CTGCTTAAGC AAAAAGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TTGCTCTGTT GCAAAATATG CTTTTTTATT TTGAGAGCGA GTCCAGCTCG 
CGACCCTCGG GATTATACCT GTTGGAGGGA TGTGTGTGTG ACAGGTCGCC CTCACCCAAA 
CCTTCACTGT CAGCCAAGGA GTGCTTAGAA AAACAGTATT ATTTCACAGT CAGCTTCAAC 
CATGAGAATC AGAAGGCCCT GGAGCTTCGC ACAGAAGATG TTAAGGACTG CGATGAATGG 
GTGGCAGCAA TATCACATGC CAGCTACAGA AACCTGGCCA CCGAGCATGA AACGCTCATG 
CAGAAGTACC TCCATCTACT CCAAATCGTG GAGACCGAGA AAACAGTTGC TAAGCAACTT 
CGACAACAGA TAGAAGATGG GGAGATTGAG ATTGAACGTC TAAAATCAGA GATTGCAGCA 
ATGCTCAAAG ACAATGAAAA GATTCAGTCA AGTCCCACCA CAGCACCCAG CGATGATGAT 
TCGGAGATCA AGAAAATCAA GAAAGTGCAA AGCTTCCTGA GAGGCTGGAT GTGTCGGCGG 
AAGTGGAAGA CCATCATTCA GGACTACATC CGTTCTCCAC ATGCCGAGAG CATGAGGAAG 
AGAAACCAGG TGGTCTTTAG CATGCTGGAG GCGGAGGCTG AGTACGTCCA GCAGCTCCAC 
ATCCTTGTCA ACAACTTCTT GAGACCGCTG CGCATGGCTG CCAGCTCCAA AAAGCCACCC 
ATAACCCACG ATGATGTCAG CAGCATATTC CTCAACAGTG AAACCATAAT GTTTCTGCAT 
CAGATATTCT ACCAAGGGTT GAAAGCTCGA ATAGCAAGCT GGCCAACATT AGTCTTGGCT 
GACTTGTTTG ACATCCTGTT GCCCATGCTG AACATCTACC AGGAGTTTGT GCGTAATCAC 
CAGTACAGCT TGCAGATCTT GGCCCACTGC AAGCAGAACC GCGATTTCGA CAAGCTGCTG 
AAACAGTATG AAGCCAAGCC TGACTGTGAG GAACGCACGC TGGAGACGTT CCTTACTTAT 
CCCATGTTTC AGATTCCTCG TTACATACTG ACCCTCCATG AGCTGTTAGC ACATACACCG 
CATGAGCATG TGGAGAGAAA CAGCTTGGAT TACGCCAAGT CGAAGTTGGA GGAGCTTTCC 
AGAATCATGC ATGATGAGGT GAGCGAGACG GAGAACATCC GGAAGAACCT CGCTATCGAG 
CGCATGATCG TGGAGGGCTG TGAGATTCTC CTAGACACCA GCCAGACCTT TGTCAGACAA 
GGATCTCTGA TTCAAGTGCC CATGAGTGAG AAGGGCAAGA TCACCCGGGG GCGACTGGGG 
TCTCTGTCTC TAAAGAAAGA GGGTGAGAGG CAGTGTTTCC TCTTCTCCAA ACATCTAATC 
ATCTGCACTA GAGGCTCTGG AGGGAAACTG CACCTCACCA AGAACGGAGT GGTGTCGCTC 
ATTGACTGCA CACTGATAGA GGAACCAGAG GGCACTGATG ACGAGTCCAA ATCAGAGAAG 
AGCGGTCAGG ACATGGAGCA CCTGGATTTT AAGATTGTGG TGGAGCCCAA AGACAGCCAG 
TCATTCACCT TCATCCTGGT GGCCTCCTCG CGGCAAGAGA AGTCAGCCTG GACCAGTGAC 
ATCAGCCAGT GCATAGATAA TATTCGCTGT AATGGGCTGA TGATGAACGC CTTTGAGGAG 
AACTCTAAAG TCACTGTGCC ACAGATGATC AAGTCTGATG CCAGTCTGTA CTGTGATGAC 
GTAGACATCC GCTTCAGTAA GATGATGAAC TCGTGCAAGG TGTTGCAGAT CCGCTATGCC 
AGTGTGGAGC GTCTGCTGGA GAGGCTGACC GACCTGCGCT TCCTCTCCAT TGACTTTCTC 
AACACCTTCC TGCACTCCTA CCGCGTGTTC ACCAGTGCTG ACGTCGTTCT GGACAAGCTC 
ATCACCATTT ACAAAAGGCC CATCAGCGCC ATACCAGCCC GATCCCTGGA GCTGTTTTTT 
GCGAGCAGCC AGAACAACAA GCTCCTCTAT GGAGAGCCTC CCAAGTCGCC CCGTGCCAGC 
CGCAAGTTTT CGTCCCCACC GCCACTGGCC ATCACTAAGA CTTCATCTCC CAATCGCCGC 
CGCAAACTGT CCCTCAACAT CCCCATCATC ACCGGAGGGA AGGCTCTGGA TCTGGCAGCC 
CTCAGCTGTT CCTCCAATGG CTATGCAAGC ATGTACTCTA CCATGTCCCC CTTCAGCAAG 
ACCACTCTGG ACATCAACAA GCTCTATGTG TCCAGTCCCA TCACCAGCAA GATCCCCGAC 
GAGGGAGAGG GCAAGACGGA CAAGGCAGAT GAAATTTCTC TCTGCAAACA AGACAATTCA 
GTGAGGGAGG AAAGCGATAA CGACCAGATC CAGAGTGATG AGGCAGAGGC TGAAGTGTCG 
CCCACCAAAT CACCCACCAC TCCCAAAAAC GTCAAATGCA AAAACTCCTC AGAATTCTCT 
CTGTTCTCTT ACAATAATGG CATGGTGATG TCATCCTGCC GGGAGCTGGA TAACAACCGT 
AGCGCCTTGT CTGCCGCTTC TGCCTTCGCC ATCGCCACTG CAGGTGCCAA CGAGGGCACG 
CCAACCAAGG AGAAGTACCG CCGCATGTCT TTGGCAAGCA CAGGGTTCCC TACGGATCAG 
AGGAACGGAG ATAAGGAGTT TGTGATTCGC CGAGCAGCAA CTAACAGAGT CCTCAATGTC 
CTCAGGCACT GGGTGTCCAA ACACTCACAG GACTTTGAAA CCAACACTGA GCTCAAGATG 
AAGGTAATCA GCTTCCTGGA GGAGGTGATG CACGATCCTG AACTTCTGAC CCAAGAAAGA 
AAGGCTGCTG CCAACATTAT ACGAACACTA ACGCAAGAGG ATCCTGGTGA TAATCAGATC 
TGTCTGGAGG AGGTTCTGCA GATGGCTGAA GGAGGAAAGT CAGAGTCTTT CGAGAACCAT 
TCAGCTCTGG AGATTGCAGA GCAGCTCACC CTACTTGACC ACCTTGTATT CAAAGTCATA 
CCATATGAAG AGTTTTTTGG TCAGGGATGG ATGAAGAATG ACAAAAATGA AAAGACACCT 
TACATCATGA AAACAACAAA ACACTTCAAC GATATCAGTG ACCTGATCGC TACAGAGATC 
CTGCGCTGTG AGGATGTGAA TGTACGGGTA GCAGTGATGG AGAAGTGGGT GGCGGTGGCT 
GATATCTGCC GCTGTCTCCA CAACTACAAT GCTGTGCTGG AAATCACCTC GTCCCTCAAT 
CGCAGCTCCA TCTTCCGCCT GAAGAAAACC TGGCTCAAAG TCTCCAAGCA GACCAAAACA 
GTCATTGACA AGCTGCAGAA GTTGGTCTCG TCTGAAGGAA GGTTCAAGAA CCTCAGAGAG 
GCCTTAAAAA ATTGTGACCC TCCCTGTGTG CCATATTTGG GAATGTATCT CACTGACTTG 
GCCTTTATTG AGGAGGGGAC GCCCAACTAC ACAGAGGACA ACCTGGTCAA TTTTTCCAAG 
ATGCGAATGA TTTCCCATAT CATCCGAGAA ATCAGGCAGT TTCAGCAAAC GGCGTATAAG 
ATTGATTACC AGCCGAAGGC AGCACTATAT TTACTGGACA GAAGCGCAGT AATGGAAGAA 
GAAGGCCTGT ATGAAGCCTC TCTCAGAATT GAGCCTAAAG TGCCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-234p18.3 ( XM_009293629.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291904.1 Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X1, mRNA.

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CloneID	ODa45587
Clone ID Related Accession (Same CDS sequence)	XM_009293629.3 , XM_009293629.2
Accession Version	XM_009293629.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293629.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GAATTCGGCT GAATGATGGT CATGTCACCT ACCTGGGTCT TTTGGCGAAG 
AAGGATGGCA CGAGGCGAGG CTGCTTAAGC AAAAAGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TTGCTCTGTT GCAAAATATG CTTTTTTATT TTGAGAGCGA GTCCAGCTCG 
CGACCCTCGG GATTATACCT GTTGGAGGGA TGTGTGTGTG ACAGGTCGCC CTCACCCAAA 
CCTTCACTGT CAGCCAAGGA GTGCTTAGAA AAACAGTATT ATTTCACAGT CAGCTTCAAC 
CATGAGAATC AGAAGGCCCT GGAGCTTCGC ACAGAAGATG TTAAGGACTG CGATGAATGG 
GTGGCAGCAA TATCACATGC CAGCTACAGA AACCTGGCCA CCGAGCATGA AACGCTCATG 
CAGAAGTACC TCCATCTACT CCAAATCGTG GAGACCGAGA AAACAGTTGC TAAGCAACTT 
CGACAACAGA TAGAAGATGG GGAGATTGAG ATTGAACGTC TAAAATCAGA GATTGCAGCA 
ATGCTCAAAG ACAATGAAAA GATTCAGTCA AGTCCCACCA CAGCACCCAG CGATGATGAT 
TCGGAGATCA AGAAAATCAA GAAAGTGCAA AGCTTCCTGA GAGGCTGGAT GTGTCGGCGG 
AAGTGGAAGA CCATCATTCA GGACTACATC CGTTCTCCAC ATGCCGAGAG CATGAGGAAG 
AGAAACCAGG TGGTCTTTAG CATGCTGGAG GCGGAGGCTG AGTACGTCCA GCAGCTCCAC 
ATCCTTGTCA ACAACTTCTT GAGACCGCTG CGCATGGCTG CCAGCTCCAA AAAGCCACCC 
ATAACCCACG ATGATGTCAG CAGCATATTC CTCAACAGTG AAACCATAAT GTTTCTGCAT 
CAGATATTCT ACCAAGGGTT GAAAGCTCGA ATAGCAAGCT GGCCAACATT AGTCTTGGCT 
GACTTGTTTG ACATCCTGTT GCCCATGCTG AACATCTACC AGGAGTTTGT GCGTAATCAC 
CAGTACAGCT TGCAGATCTT GGCCCACTGC AAGCAGAACC GCGATTTCGA CAAGCTGCTG 
AAACAGTATG AAGCCAAGCC TGACTGTGAG GAACGCACGC TGGAGACGTT CCTTACTTAT 
CCCATGTTTC AGATTCCTCG TTACATACTG ACCCTCCATG AGCTGTTAGC ACATACACCG 
CATGAGCATG TGGAGAGAAA CAGCTTGGAT TACGCCAAGT CGAAGTTGGA GGAGCTTTCC 
AGAATCATGC ATGATGAGGT GAGCGAGACG GAGAACATCC GGAAGAACCT CGCTATCGAG 
CGCATGATCG TGGAGGGCTG TGAGATTCTC CTAGACACCA GCCAGACCTT TGTCAGACAA 
GGATCTCTGA TTCAAGTGCC CATGAGTGAG AAGGGCAAGA TCACCCGGGG GCGACTGGGG 
TCTCTGTCTC TAAAGAAAGA GGGTGAGAGG CAGTGTTTCC TCTTCTCCAA ACATCTAATC 
ATCTGCACTA GAGGCTCTGG AGGGAAACTG CACCTCACCA AGGTGAAGCC TCATACAGTA 
CAAAACGGAG TGGTGTCGCT CATTGACTGC ACACTGATAG AGGAACCAGA GGGCACTGAT 
GACGAGTCCA AATCAGAGAA GAGCGGTCAG GACATGGAGC ACCTGGATTT TAAGATTGTG 
GTGGAGCCCA AAGACAGCCA GTCATTCACC TTCATCCTGG TGGCCTCCTC GCGGCAAGAG 
AAGTCAGCCT GGACCAGTGA CATCAGCCAG TGCATAGATA ATATTCGCTG TAATGGGCTG 
ATGATGAACG CCTTTGAGGA GAACTCTAAA GTCACTGTGC CACAGATGAT CAAGTCTGAT 
GCCAGTCTGT ACTGTGATGA CGTAGACATC CGCTTCAGTA AGATGATGAA CTCGTGCAAG 
GTGTTGCAGA TCCGCTATGC CAGTGTGGAG CGTCTGCTGG AGAGGCTGAC CGACCTGCGC 
TTCCTCTCCA TTGACTTTCT CAACACCTTC CTGCACTCCT ACCGCGTGTT CACCAGTGCT 
GACGTCGTTC TGGACAAGCT CATCACCATT TACAAAAGGC CCATCAGCGC CATACCAGCC 
CGATCCCTGG AGCTGTTTTT TGCGAGCAGC CAGAACAACA AGCTCCTCTA TGGAGAGCCT 
CCCAAGTCGC CCCGTGCCAG CCGCAAGTTT TCGTCCCCAC CGCCACTGGC CATCACTAAG 
ACTTCATCTC CCAATCGCCG CCGCAAACTG TCCCTCAACA TCCCCATCAT CACCGGAGGG 
AAGGCTCTGG ATCTGGCAGC CCTCAGCTGT TCCTCCAATG GCTATGCAAG CATGTACTCT 
ACCATGTCCC CCTTCAGCAA GACCACTCTG GACATCAACA AGCTCTATGT GTCCAGTCCC 
ATCACCAGCA AGATCCCCGA CGAGGGAGAG GGCAAGACGG ACAAGGCAGA TGAAATTTCT 
CTCTGCAAAC AAGACAATTC AGTGAGGGAG GAAAGCGATA ACGACCAGAT CCAGAGTGAT 
GAGGCAGAGG CTGAAGTGTC GCCCACCAAA TCACCCACCA CTCCCAAAAA CGTCAAATGC 
AAAAACTCCT CAGAATTCTC TCTGTTCTCT TACAATAATG GCATGGTGAT GTCATCCTGC 
CGGGAGCTGG ATAACAACCG TAGCGCCTTG TCTGCCGCTT CTGCCTTCGC CATCGCCACT 
GCAGGTGCCA ACGAGGGCAC GCCAACCAAG GAGAAGTACC GCCGCATGTC TTTGGCAAGC 
ACAGGGTTCC CTACGGATCA GAGGAACGGA GATAAGGAGT TTGTGATTCG CCGAGCAGCA 
ACTAACAGAG TCCTCAATGT CCTCAGGCAC TGGGTGTCCA AACACTCACA GGACTTTGAA 
ACCAACACTG AGCTCAAGAT GAAGGTAATC AGCTTCCTGG AGGAGGTGAT GCACGATCCT 
GAACTTCTGA CCCAAGAAAG AAAGGCTGCT GCCAACATTA TACGAACACT AACGCAAGAG 
GATCCTGGTG ATAATCAGAT CTGTCTGGAG GAGGTTCTGC AGATGGCTGA AGGAGGAAAG 
TCAGAGTCTT TCGAGAACCA TTCAGCTCTG GAGATTGCAG AGCAGCTCAC CCTACTTGAC 
CACCTTGTAT TCAAAGTCAT ACCATATGAA GAGTTTTTTG GTCAGGGATG GATGAAGAAT 
GACAAAAATG AAAAGACACC TTACATCATG AAAACAACAA AACACTTCAA CGATATCAGT 
GACCTGATCG CTACAGAGAT CCTGCGCTGT GAGGATGTGA ATGTACGGGT AGCAGTGATG 
GAGAAGTGGG TGGCGGTGGC TGATATCTGC CGCTGTCTCC ACAACTACAA TGCTGTGCTG 
GAAATCACCT CGTCCCTCAA TCGCAGCTCC ATCTTCCGCC TGAAGAAAAC CTGGCTCAAA 
GTCTCCAAGC AGACCAAAAC AGTCATTGAC AAGCTGCAGA AGTTGGTCTC GTCTGAAGGA 
AGGTTCAAGA ACCTCAGAGA GGCCTTAAAA AATTGTGACC CTCCCTGTGT GCCATATTTG 
GGAATGTATC TCACTGACTT GGCCTTTATT GAGGAGGGGA CGCCCAACTA CACAGAGGAC 
AACCTGGTCA ATTTTTCCAA GATGCGAATG ATTTCCCATA TCATCCGAGA AATCAGGCAG 
TTTCAGCAAA CGGCGTATAA GATTGATTAC CAGCCGAAGG CAGCACTATA TTTACTGGAC 
AGAAGCGCAG TAATGGAAGA AGAAGGCCTG TATGAAGCCT CTCTCAGAAT TGAGCCTAAA 
GTGCCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291904.1
CDS	376..4167
Translation	MQKGIRLNDGHVTYLGLLAKKDGTRRGCLSKKSSDNTKWHTKWFALLQNMLFYFESESSSRPSGLYLLEGCVCDRSPSPKPSLSAKECLEKQYYFTVSFNHENQKALELRTEDVKDCDEWVAAISHASYRNLATEHETLMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKSEIAAMLKDNEKIQSSPTTAPSDDDSEIKKIKKVQSFLRGWMCRRKWKTIIQDYIRSPHAESMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIVEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKVKPHTVQNGVVSLIDCTLIEEPEGTDDESKSEKSGQDMEHLDFKIVVEPKDSQSFTFILVASSRQEKSAWTSDISQCIDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKMMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTSADVVLDKLITIYKRPISAIPARSLELFFASSQNNKLLYGEPPKSPRASRKFSSPPPLAITKTSSPNRRRKLSLNIPIITGGKALDLAALSCSSNGYASMYSTMSPFSKTTLDINKLYVSSPITSKIPDEGEGKTDKADEISLCKQDNSVREESDNDQIQSDEAEAEVSPTKSPTTPKNVKCKNSSEFSLFSYNNGMVMSSCRELDNNRSALSAASAFAIATAGANEGTPTKEKYRRMSLASTGFPTDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNTELKMKVISFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQICLEEVLQMAEGGKSESFENHSALEIAEQLTLLDHLVFKVIPYEEFFGQGWMKNDKNEKTPYIMKTTKHFNDISDLIATEILRCEDVNVRVAVMEKWVAVADICRCLHNYNAVLEITSSLNRSSIFRLKKTWLKVSKQTKTVIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDNLVNFSKMRMISHIIREIRQFQQTAYKIDYQPKAALYLLDRSAVMEEEGLYEASLRIEPKVPN

Target ORF information:

RefSeq Version	XM_009293629.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293629.3

ORF Insert Sequence:

ATGCAGAAGG GAATTCGGCT GAATGATGGT CATGTCACCT ACCTGGGTCT TTTGGCGAAG 
AAGGATGGCA CGAGGCGAGG CTGCTTAAGC AAAAAGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TTGCTCTGTT GCAAAATATG CTTTTTTATT TTGAGAGCGA GTCCAGCTCG 
CGACCCTCGG GATTATACCT GTTGGAGGGA TGTGTGTGTG ACAGGTCGCC CTCACCCAAA 
CCTTCACTGT CAGCCAAGGA GTGCTTAGAA AAACAGTATT ATTTCACAGT CAGCTTCAAC 
CATGAGAATC AGAAGGCCCT GGAGCTTCGC ACAGAAGATG TTAAGGACTG CGATGAATGG 
GTGGCAGCAA TATCACATGC CAGCTACAGA AACCTGGCCA CCGAGCATGA AACGCTCATG 
CAGAAGTACC TCCATCTACT CCAAATCGTG GAGACCGAGA AAACAGTTGC TAAGCAACTT 
CGACAACAGA TAGAAGATGG GGAGATTGAG ATTGAACGTC TAAAATCAGA GATTGCAGCA 
ATGCTCAAAG ACAATGAAAA GATTCAGTCA AGTCCCACCA CAGCACCCAG CGATGATGAT 
TCGGAGATCA AGAAAATCAA GAAAGTGCAA AGCTTCCTGA GAGGCTGGAT GTGTCGGCGG 
AAGTGGAAGA CCATCATTCA GGACTACATC CGTTCTCCAC ATGCCGAGAG CATGAGGAAG 
AGAAACCAGG TGGTCTTTAG CATGCTGGAG GCGGAGGCTG AGTACGTCCA GCAGCTCCAC 
ATCCTTGTCA ACAACTTCTT GAGACCGCTG CGCATGGCTG CCAGCTCCAA AAAGCCACCC 
ATAACCCACG ATGATGTCAG CAGCATATTC CTCAACAGTG AAACCATAAT GTTTCTGCAT 
CAGATATTCT ACCAAGGGTT GAAAGCTCGA ATAGCAAGCT GGCCAACATT AGTCTTGGCT 
GACTTGTTTG ACATCCTGTT GCCCATGCTG AACATCTACC AGGAGTTTGT GCGTAATCAC 
CAGTACAGCT TGCAGATCTT GGCCCACTGC AAGCAGAACC GCGATTTCGA CAAGCTGCTG 
AAACAGTATG AAGCCAAGCC TGACTGTGAG GAACGCACGC TGGAGACGTT CCTTACTTAT 
CCCATGTTTC AGATTCCTCG TTACATACTG ACCCTCCATG AGCTGTTAGC ACATACACCG 
CATGAGCATG TGGAGAGAAA CAGCTTGGAT TACGCCAAGT CGAAGTTGGA GGAGCTTTCC 
AGAATCATGC ATGATGAGGT GAGCGAGACG GAGAACATCC GGAAGAACCT CGCTATCGAG 
CGCATGATCG TGGAGGGCTG TGAGATTCTC CTAGACACCA GCCAGACCTT TGTCAGACAA 
GGATCTCTGA TTCAAGTGCC CATGAGTGAG AAGGGCAAGA TCACCCGGGG GCGACTGGGG 
TCTCTGTCTC TAAAGAAAGA GGGTGAGAGG CAGTGTTTCC TCTTCTCCAA ACATCTAATC 
ATCTGCACTA GAGGCTCTGG AGGGAAACTG CACCTCACCA AGGTGAAGCC TCATACAGTA 
CAAAACGGAG TGGTGTCGCT CATTGACTGC ACACTGATAG AGGAACCAGA GGGCACTGAT 
GACGAGTCCA AATCAGAGAA GAGCGGTCAG GACATGGAGC ACCTGGATTT TAAGATTGTG 
GTGGAGCCCA AAGACAGCCA GTCATTCACC TTCATCCTGG TGGCCTCCTC GCGGCAAGAG 
AAGTCAGCCT GGACCAGTGA CATCAGCCAG TGCATAGATA ATATTCGCTG TAATGGGCTG 
ATGATGAACG CCTTTGAGGA GAACTCTAAA GTCACTGTGC CACAGATGAT CAAGTCTGAT 
GCCAGTCTGT ACTGTGATGA CGTAGACATC CGCTTCAGTA AGATGATGAA CTCGTGCAAG 
GTGTTGCAGA TCCGCTATGC CAGTGTGGAG CGTCTGCTGG AGAGGCTGAC CGACCTGCGC 
TTCCTCTCCA TTGACTTTCT CAACACCTTC CTGCACTCCT ACCGCGTGTT CACCAGTGCT 
GACGTCGTTC TGGACAAGCT CATCACCATT TACAAAAGGC CCATCAGCGC CATACCAGCC 
CGATCCCTGG AGCTGTTTTT TGCGAGCAGC CAGAACAACA AGCTCCTCTA TGGAGAGCCT 
CCCAAGTCGC CCCGTGCCAG CCGCAAGTTT TCGTCCCCAC CGCCACTGGC CATCACTAAG 
ACTTCATCTC CCAATCGCCG CCGCAAACTG TCCCTCAACA TCCCCATCAT CACCGGAGGG 
AAGGCTCTGG ATCTGGCAGC CCTCAGCTGT TCCTCCAATG GCTATGCAAG CATGTACTCT 
ACCATGTCCC CCTTCAGCAA GACCACTCTG GACATCAACA AGCTCTATGT GTCCAGTCCC 
ATCACCAGCA AGATCCCCGA CGAGGGAGAG GGCAAGACGG ACAAGGCAGA TGAAATTTCT 
CTCTGCAAAC AAGACAATTC AGTGAGGGAG GAAAGCGATA ACGACCAGAT CCAGAGTGAT 
GAGGCAGAGG CTGAAGTGTC GCCCACCAAA TCACCCACCA CTCCCAAAAA CGTCAAATGC 
AAAAACTCCT CAGAATTCTC TCTGTTCTCT TACAATAATG GCATGGTGAT GTCATCCTGC 
CGGGAGCTGG ATAACAACCG TAGCGCCTTG TCTGCCGCTT CTGCCTTCGC CATCGCCACT 
GCAGGTGCCA ACGAGGGCAC GCCAACCAAG GAGAAGTACC GCCGCATGTC TTTGGCAAGC 
ACAGGGTTCC CTACGGATCA GAGGAACGGA GATAAGGAGT TTGTGATTCG CCGAGCAGCA 
ACTAACAGAG TCCTCAATGT CCTCAGGCAC TGGGTGTCCA AACACTCACA GGACTTTGAA 
ACCAACACTG AGCTCAAGAT GAAGGTAATC AGCTTCCTGG AGGAGGTGAT GCACGATCCT 
GAACTTCTGA CCCAAGAAAG AAAGGCTGCT GCCAACATTA TACGAACACT AACGCAAGAG 
GATCCTGGTG ATAATCAGAT CTGTCTGGAG GAGGTTCTGC AGATGGCTGA AGGAGGAAAG 
TCAGAGTCTT TCGAGAACCA TTCAGCTCTG GAGATTGCAG AGCAGCTCAC CCTACTTGAC 
CACCTTGTAT TCAAAGTCAT ACCATATGAA GAGTTTTTTG GTCAGGGATG GATGAAGAAT 
GACAAAAATG AAAAGACACC TTACATCATG AAAACAACAA AACACTTCAA CGATATCAGT 
GACCTGATCG CTACAGAGAT CCTGCGCTGT GAGGATGTGA ATGTACGGGT AGCAGTGATG 
GAGAAGTGGG TGGCGGTGGC TGATATCTGC CGCTGTCTCC ACAACTACAA TGCTGTGCTG 
GAAATCACCT CGTCCCTCAA TCGCAGCTCC ATCTTCCGCC TGAAGAAAAC CTGGCTCAAA 
GTCTCCAAGC AGACCAAAAC AGTCATTGAC AAGCTGCAGA AGTTGGTCTC GTCTGAAGGA 
AGGTTCAAGA ACCTCAGAGA GGCCTTAAAA AATTGTGACC CTCCCTGTGT GCCATATTTG 
GGAATGTATC TCACTGACTT GGCCTTTATT GAGGAGGGGA CGCCCAACTA CACAGAGGAC 
AACCTGGTCA ATTTTTCCAA GATGCGAATG ATTTCCCATA TCATCCGAGA AATCAGGCAG 
TTTCAGCAAA CGGCGTATAA GATTGATTAC CAGCCGAAGG CAGCACTATA TTTACTGGAC 
AGAAGCGCAG TAATGGAAGA AGAAGGCCTG TATGAAGCCT CTCTCAGAAT TGAGCCTAAA 
GTGCCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-234p18.3 ( XM_009293629.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291904.1 Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X1, mRNA.

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CloneID	ODa45587
Clone ID Related Accession (Same CDS sequence)	XM_009293629.3 , XM_009293629.2
Accession Version	XM_009293629.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GAATTCGGCT GAATGATGGT CATGTCACCT ACCTGGGTCT TTTGGCGAAG 
AAGGATGGCA CGAGGCGAGG CTGCTTAAGC AAAAAGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TTGCTCTGTT GCAAAATATG CTTTTTTATT TTGAGAGCGA GTCCAGCTCG 
CGACCCTCGG GATTATACCT GTTGGAGGGA TGTGTGTGTG ACAGGTCGCC CTCACCCAAA 
CCTTCACTGT CAGCCAAGGA GTGCTTAGAA AAACAGTATT ATTTCACAGT CAGCTTCAAC 
CATGAGAATC AGAAGGCCCT GGAGCTTCGC ACAGAAGATG TTAAGGACTG CGATGAATGG 
GTGGCAGCAA TATCACATGC CAGCTACAGA AACCTGGCCA CCGAGCATGA AACGCTCATG 
CAGAAGTACC TCCATCTACT CCAAATCGTG GAGACCGAGA AAACAGTTGC TAAGCAACTT 
CGACAACAGA TAGAAGATGG GGAGATTGAG ATTGAACGTC TAAAATCAGA GATTGCAGCA 
ATGCTCAAAG ACAATGAAAA GATTCAGTCA AGTCCCACCA CAGCACCCAG CGATGATGAT 
TCGGAGATCA AGAAAATCAA GAAAGTGCAA AGCTTCCTGA GAGGCTGGAT GTGTCGGCGG 
AAGTGGAAGA CCATCATTCA GGACTACATC CGTTCTCCAC ATGCCGAGAG CATGAGGAAG 
AGAAACCAGG TGGTCTTTAG CATGCTGGAG GCGGAGGCTG AGTACGTCCA GCAGCTCCAC 
ATCCTTGTCA ACAACTTCTT GAGACCGCTG CGCATGGCTG CCAGCTCCAA AAAGCCACCC 
ATAACCCACG ATGATGTCAG CAGCATATTC CTCAACAGTG AAACCATAAT GTTTCTGCAT 
CAGATATTCT ACCAAGGGTT GAAAGCTCGA ATAGCAAGCT GGCCAACATT AGTCTTGGCT 
GACTTGTTTG ACATCCTGTT GCCCATGCTG AACATCTACC AGGAGTTTGT GCGTAATCAC 
CAGTACAGCT TGCAGATCTT GGCCCACTGC AAGCAGAACC GCGATTTCGA CAAGCTGCTG 
AAACAGTATG AAGCCAAGCC TGACTGTGAG GAACGCACGC TGGAGACGTT CCTTACTTAT 
CCCATGTTTC AGATTCCTCG TTACATACTG ACCCTCCATG AGCTGTTAGC ACATACACCG 
CATGAGCATG TGGAGAGAAA CAGCTTGGAT TACGCCAAGT CGAAGTTGGA GGAGCTTTCC 
AGAATCATGC ATGATGAGGT GAGCGAGACG GAGAACATCC GGAAGAACCT CGCTATCGAG 
CGCATGATCG TGGAGGGCTG TGAGATTCTC CTAGACACCA GCCAGACCTT TGTCAGACAA 
GGATCTCTGA TTCAAGTGCC CATGAGTGAG AAGGGCAAGA TCACCCGGGG GCGACTGGGG 
TCTCTGTCTC TAAAGAAAGA GGGTGAGAGG CAGTGTTTCC TCTTCTCCAA ACATCTAATC 
ATCTGCACTA GAGGCTCTGG AGGGAAACTG CACCTCACCA AGGTGAAGCC TCATACAGTA 
CAAAACGGAG TGGTGTCGCT CATTGACTGC ACACTGATAG AGGAACCAGA GGGCACTGAT 
GACGAGTCCA AATCAGAGAA GAGCGGTCAG GACATGGAGC ACCTGGATTT TAAGATTGTG 
GTGGAGCCCA AAGACAGCCA GTCATTCACC TTCATCCTGG TGGCCTCCTC GCGGCAAGAG 
AAGTCAGCCT GGACCAGTGA CATCAGCCAG TGCATAGATA ATATTCGCTG TAATGGGCTG 
ATGATGAACG CCTTTGAGGA GAACTCTAAA GTCACTGTGC CACAGATGAT CAAGTCTGAT 
GCCAGTCTGT ACTGTGATGA CGTAGACATC CGCTTCAGTA AGATGATGAA CTCGTGCAAG 
GTGTTGCAGA TCCGCTATGC CAGTGTGGAG CGTCTGCTGG AGAGGCTGAC CGACCTGCGC 
TTCCTCTCCA TTGACTTTCT CAACACCTTC CTGCACTCCT ACCGCGTGTT CACCAGTGCT 
GACGTCGTTC TGGACAAGCT CATCACCATT TACAAAAGGC CCATCAGCGC CATACCAGCC 
CGATCCCTGG AGCTGTTTTT TGCGAGCAGC CAGAACAACA AGCTCCTCTA TGGAGAGCCT 
CCCAAGTCGC CCCGTGCCAG CCGCAAGTTT TCGTCCCCAC CGCCACTGGC CATCACTAAG 
ACTTCATCTC CCAATCGCCG CCGCAAACTG TCCCTCAACA TCCCCATCAT CACCGGAGGG 
AAGGCTCTGG ATCTGGCAGC CCTCAGCTGT TCCTCCAATG GCTATGCAAG CATGTACTCT 
ACCATGTCCC CCTTCAGCAA GACCACTCTG GACATCAACA AGCTCTATGT GTCCAGTCCC 
ATCACCAGCA AGATCCCCGA CGAGGGAGAG GGCAAGACGG ACAAGGCAGA TGAAATTTCT 
CTCTGCAAAC AAGACAATTC AGTGAGGGAG GAAAGCGATA ACGACCAGAT CCAGAGTGAT 
GAGGCAGAGG CTGAAGTGTC GCCCACCAAA TCACCCACCA CTCCCAAAAA CGTCAAATGC 
AAAAACTCCT CAGAATTCTC TCTGTTCTCT TACAATAATG GCATGGTGAT GTCATCCTGC 
CGGGAGCTGG ATAACAACCG TAGCGCCTTG TCTGCCGCTT CTGCCTTCGC CATCGCCACT 
GCAGGTGCCA ACGAGGGCAC GCCAACCAAG GAGAAGTACC GCCGCATGTC TTTGGCAAGC 
ACAGGGTTCC CTACGGATCA GAGGAACGGA GATAAGGAGT TTGTGATTCG CCGAGCAGCA 
ACTAACAGAG TCCTCAATGT CCTCAGGCAC TGGGTGTCCA AACACTCACA GGACTTTGAA 
ACCAACACTG AGCTCAAGAT GAAGGTAATC AGCTTCCTGG AGGAGGTGAT GCACGATCCT 
GAACTTCTGA CCCAAGAAAG AAAGGCTGCT GCCAACATTA TACGAACACT AACGCAAGAG 
GATCCTGGTG ATAATCAGAT CTGTCTGGAG GAGGTTCTGC AGATGGCTGA AGGAGGAAAG 
TCAGAGTCTT TCGAGAACCA TTCAGCTCTG GAGATTGCAG AGCAGCTCAC CCTACTTGAC 
CACCTTGTAT TCAAAGTCAT ACCATATGAA GAGTTTTTTG GTCAGGGATG GATGAAGAAT 
GACAAAAATG AAAAGACACC TTACATCATG AAAACAACAA AACACTTCAA CGATATCAGT 
GACCTGATCG CTACAGAGAT CCTGCGCTGT GAGGATGTGA ATGTACGGGT AGCAGTGATG 
GAGAAGTGGG TGGCGGTGGC TGATATCTGC CGCTGTCTCC ACAACTACAA TGCTGTGCTG 
GAAATCACCT CGTCCCTCAA TCGCAGCTCC ATCTTCCGCC TGAAGAAAAC CTGGCTCAAA 
GTCTCCAAGC AGACCAAAAC AGTCATTGAC AAGCTGCAGA AGTTGGTCTC GTCTGAAGGA 
AGGTTCAAGA ACCTCAGAGA GGCCTTAAAA AATTGTGACC CTCCCTGTGT GCCATATTTG 
GGAATGTATC TCACTGACTT GGCCTTTATT GAGGAGGGGA CGCCCAACTA CACAGAGGAC 
AACCTGGTCA ATTTTTCCAA GATGCGAATG ATTTCCCATA TCATCCGAGA AATCAGGCAG 
TTTCAGCAAA CGGCGTATAA GATTGATTAC CAGCCGAAGG CAGCACTATA TTTACTGGAC 
AGAAGCGCAG TAATGGAAGA AGAAGGCCTG TATGAAGCCT CTCTCAGAAT TGAGCCTAAA 
GTGCCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291904.1
CDS	381..4172
Translation	MQKGIRLNDGHVTYLGLLAKKDGTRRGCLSKKSSDNTKWHTKWFALLQNMLFYFESESSSRPSGLYLLEGCVCDRSPSPKPSLSAKECLEKQYYFTVSFNHENQKALELRTEDVKDCDEWVAAISHASYRNLATEHETLMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKSEIAAMLKDNEKIQSSPTTAPSDDDSEIKKIKKVQSFLRGWMCRRKWKTIIQDYIRSPHAESMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIVEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKVKPHTVQNGVVSLIDCTLIEEPEGTDDESKSEKSGQDMEHLDFKIVVEPKDSQSFTFILVASSRQEKSAWTSDISQCIDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKMMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTSADVVLDKLITIYKRPISAIPARSLELFFASSQNNKLLYGEPPKSPRASRKFSSPPPLAITKTSSPNRRRKLSLNIPIITGGKALDLAALSCSSNGYASMYSTMSPFSKTTLDINKLYVSSPITSKIPDEGEGKTDKADEISLCKQDNSVREESDNDQIQSDEAEAEVSPTKSPTTPKNVKCKNSSEFSLFSYNNGMVMSSCRELDNNRSALSAASAFAIATAGANEGTPTKEKYRRMSLASTGFPTDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNTELKMKVISFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQICLEEVLQMAEGGKSESFENHSALEIAEQLTLLDHLVFKVIPYEEFFGQGWMKNDKNEKTPYIMKTTKHFNDISDLIATEILRCEDVNVRVAVMEKWVAVADICRCLHNYNAVLEITSSLNRSSIFRLKKTWLKVSKQTKTVIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDNLVNFSKMRMISHIIREIRQFQQTAYKIDYQPKAALYLLDRSAVMEEEGLYEASLRIEPKVPN

Target ORF information:

RefSeq Version	XM_009293629.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-234p18.3 (si:ch211-234p18.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293629.2

ORF Insert Sequence:

ATGCAGAAGG GAATTCGGCT GAATGATGGT CATGTCACCT ACCTGGGTCT TTTGGCGAAG 
AAGGATGGCA CGAGGCGAGG CTGCTTAAGC AAAAAGAGCT CCGACAACAC AAAATGGCAC 
ACCAAGTGGT TTGCTCTGTT GCAAAATATG CTTTTTTATT TTGAGAGCGA GTCCAGCTCG 
CGACCCTCGG GATTATACCT GTTGGAGGGA TGTGTGTGTG ACAGGTCGCC CTCACCCAAA 
CCTTCACTGT CAGCCAAGGA GTGCTTAGAA AAACAGTATT ATTTCACAGT CAGCTTCAAC 
CATGAGAATC AGAAGGCCCT GGAGCTTCGC ACAGAAGATG TTAAGGACTG CGATGAATGG 
GTGGCAGCAA TATCACATGC CAGCTACAGA AACCTGGCCA CCGAGCATGA AACGCTCATG 
CAGAAGTACC TCCATCTACT CCAAATCGTG GAGACCGAGA AAACAGTTGC TAAGCAACTT 
CGACAACAGA TAGAAGATGG GGAGATTGAG ATTGAACGTC TAAAATCAGA GATTGCAGCA 
ATGCTCAAAG ACAATGAAAA GATTCAGTCA AGTCCCACCA CAGCACCCAG CGATGATGAT 
TCGGAGATCA AGAAAATCAA GAAAGTGCAA AGCTTCCTGA GAGGCTGGAT GTGTCGGCGG 
AAGTGGAAGA CCATCATTCA GGACTACATC CGTTCTCCAC ATGCCGAGAG CATGAGGAAG 
AGAAACCAGG TGGTCTTTAG CATGCTGGAG GCGGAGGCTG AGTACGTCCA GCAGCTCCAC 
ATCCTTGTCA ACAACTTCTT GAGACCGCTG CGCATGGCTG CCAGCTCCAA AAAGCCACCC 
ATAACCCACG ATGATGTCAG CAGCATATTC CTCAACAGTG AAACCATAAT GTTTCTGCAT 
CAGATATTCT ACCAAGGGTT GAAAGCTCGA ATAGCAAGCT GGCCAACATT AGTCTTGGCT 
GACTTGTTTG ACATCCTGTT GCCCATGCTG AACATCTACC AGGAGTTTGT GCGTAATCAC 
CAGTACAGCT TGCAGATCTT GGCCCACTGC AAGCAGAACC GCGATTTCGA CAAGCTGCTG 
AAACAGTATG AAGCCAAGCC TGACTGTGAG GAACGCACGC TGGAGACGTT CCTTACTTAT 
CCCATGTTTC AGATTCCTCG TTACATACTG ACCCTCCATG AGCTGTTAGC ACATACACCG 
CATGAGCATG TGGAGAGAAA CAGCTTGGAT TACGCCAAGT CGAAGTTGGA GGAGCTTTCC 
AGAATCATGC ATGATGAGGT GAGCGAGACG GAGAACATCC GGAAGAACCT CGCTATCGAG 
CGCATGATCG TGGAGGGCTG TGAGATTCTC CTAGACACCA GCCAGACCTT TGTCAGACAA 
GGATCTCTGA TTCAAGTGCC CATGAGTGAG AAGGGCAAGA TCACCCGGGG GCGACTGGGG 
TCTCTGTCTC TAAAGAAAGA GGGTGAGAGG CAGTGTTTCC TCTTCTCCAA ACATCTAATC 
ATCTGCACTA GAGGCTCTGG AGGGAAACTG CACCTCACCA AGGTGAAGCC TCATACAGTA 
CAAAACGGAG TGGTGTCGCT CATTGACTGC ACACTGATAG AGGAACCAGA GGGCACTGAT 
GACGAGTCCA AATCAGAGAA GAGCGGTCAG GACATGGAGC ACCTGGATTT TAAGATTGTG 
GTGGAGCCCA AAGACAGCCA GTCATTCACC TTCATCCTGG TGGCCTCCTC GCGGCAAGAG 
AAGTCAGCCT GGACCAGTGA CATCAGCCAG TGCATAGATA ATATTCGCTG TAATGGGCTG 
ATGATGAACG CCTTTGAGGA GAACTCTAAA GTCACTGTGC CACAGATGAT CAAGTCTGAT 
GCCAGTCTGT ACTGTGATGA CGTAGACATC CGCTTCAGTA AGATGATGAA CTCGTGCAAG 
GTGTTGCAGA TCCGCTATGC CAGTGTGGAG CGTCTGCTGG AGAGGCTGAC CGACCTGCGC 
TTCCTCTCCA TTGACTTTCT CAACACCTTC CTGCACTCCT ACCGCGTGTT CACCAGTGCT 
GACGTCGTTC TGGACAAGCT CATCACCATT TACAAAAGGC CCATCAGCGC CATACCAGCC 
CGATCCCTGG AGCTGTTTTT TGCGAGCAGC CAGAACAACA AGCTCCTCTA TGGAGAGCCT 
CCCAAGTCGC CCCGTGCCAG CCGCAAGTTT TCGTCCCCAC CGCCACTGGC CATCACTAAG 
ACTTCATCTC CCAATCGCCG CCGCAAACTG TCCCTCAACA TCCCCATCAT CACCGGAGGG 
AAGGCTCTGG ATCTGGCAGC CCTCAGCTGT TCCTCCAATG GCTATGCAAG CATGTACTCT 
ACCATGTCCC CCTTCAGCAA GACCACTCTG GACATCAACA AGCTCTATGT GTCCAGTCCC 
ATCACCAGCA AGATCCCCGA CGAGGGAGAG GGCAAGACGG ACAAGGCAGA TGAAATTTCT 
CTCTGCAAAC AAGACAATTC AGTGAGGGAG GAAAGCGATA ACGACCAGAT CCAGAGTGAT 
GAGGCAGAGG CTGAAGTGTC GCCCACCAAA TCACCCACCA CTCCCAAAAA CGTCAAATGC 
AAAAACTCCT CAGAATTCTC TCTGTTCTCT TACAATAATG GCATGGTGAT GTCATCCTGC 
CGGGAGCTGG ATAACAACCG TAGCGCCTTG TCTGCCGCTT CTGCCTTCGC CATCGCCACT 
GCAGGTGCCA ACGAGGGCAC GCCAACCAAG GAGAAGTACC GCCGCATGTC TTTGGCAAGC 
ACAGGGTTCC CTACGGATCA GAGGAACGGA GATAAGGAGT TTGTGATTCG CCGAGCAGCA 
ACTAACAGAG TCCTCAATGT CCTCAGGCAC TGGGTGTCCA AACACTCACA GGACTTTGAA 
ACCAACACTG AGCTCAAGAT GAAGGTAATC AGCTTCCTGG AGGAGGTGAT GCACGATCCT 
GAACTTCTGA CCCAAGAAAG AAAGGCTGCT GCCAACATTA TACGAACACT AACGCAAGAG 
GATCCTGGTG ATAATCAGAT CTGTCTGGAG GAGGTTCTGC AGATGGCTGA AGGAGGAAAG 
TCAGAGTCTT TCGAGAACCA TTCAGCTCTG GAGATTGCAG AGCAGCTCAC CCTACTTGAC 
CACCTTGTAT TCAAAGTCAT ACCATATGAA GAGTTTTTTG GTCAGGGATG GATGAAGAAT 
GACAAAAATG AAAAGACACC TTACATCATG AAAACAACAA AACACTTCAA CGATATCAGT 
GACCTGATCG CTACAGAGAT CCTGCGCTGT GAGGATGTGA ATGTACGGGT AGCAGTGATG 
GAGAAGTGGG TGGCGGTGGC TGATATCTGC CGCTGTCTCC ACAACTACAA TGCTGTGCTG 
GAAATCACCT CGTCCCTCAA TCGCAGCTCC ATCTTCCGCC TGAAGAAAAC CTGGCTCAAA 
GTCTCCAAGC AGACCAAAAC AGTCATTGAC AAGCTGCAGA AGTTGGTCTC GTCTGAAGGA 
AGGTTCAAGA ACCTCAGAGA GGCCTTAAAA AATTGTGACC CTCCCTGTGT GCCATATTTG 
GGAATGTATC TCACTGACTT GGCCTTTATT GAGGAGGGGA CGCCCAACTA CACAGAGGAC 
AACCTGGTCA ATTTTTCCAA GATGCGAATG ATTTCCCATA TCATCCGAGA AATCAGGCAG 
TTTCAGCAAA CGGCGTATAA GATTGATTAC CAGCCGAAGG CAGCACTATA TTTACTGGAC 
AGAAGCGCAG TAATGGAAGA AGAAGGCCTG TATGAAGCCT CTCTCAGAAT TGAGCCTAAA 
GTGCCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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si:ch211-234p18.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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