Rasgrf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasgrf1
Entrez Gene ID	192213
Full Name	RAS protein-specific guanine nucleotide-releasing factor 1
Synonyms	GNRP,P140 RAS-GRF
General protein information	Preferred Names RAS protein-specific guanine nucleotide-releasing factor 1 Names ras-specific guanine nucleotide-releasing factor 1 guanine nucleotide-releasing protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31
Summary	a paternally expressed imprinted gene [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105753.1	NP_001099223.1	ras-specific guanine nucleotide-releasing factor 1 isoform 2
NM_001170531.1	NP_001164002.1	ras-specific guanine nucleotide-releasing factor 1 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RASGRF1	NP_002882.3
Mus musculus (house mouse)	Rasgrf1	NP_035375.1
Danio rerio (zebrafish)	si:ch211-234p18.3	XP_002665395.1
Xenopus tropicalis (tropical clawed frog)	rasgrf1	XP_002933009.2
Gallus gallus (chicken)	RASGRF1	XP_004944008.1
Pan troglodytes (chimpanzee)	RASGRF1	XP_001153586.1
Canis lupus familiaris (dog)	RASGRF1	XP_545892.5
Rattus norvegicus (Norway rat)	Rasgrf1	NP_001164002.1
Bos taurus (cattle)	RASGRF1	NP_001178386.1

Gene Ontology
Bibliography

Related articles in PubMed

RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.
Wu RF, Liao C, Hatoum H, Fu G, Ochoa CD, Terada LS
Arteriosclerosis, thrombosis, and vascular biology37(1)98-107(2017 01)

A Mercaptoacetamide-Based Class II Histone Deacetylase Inhibitor Increases Dendritic Spine Density via RasGRF1/ERK Pathway.
Song JM, Sung YM, Nam JH, Yoon H, Chung A, Moffat E, Jung M, Pak DT, Kim J, Hoe HS
Journal of Alzheimer's disease : JAD51(2)591-604(2016)

Very low density lipoprotein receptor regulates dendritic spine formation in a RasGRF1/CaMKII dependent manner.
DiBattista AM, Dumanis SB, Song JM, Bu G, Weeber E, Rebeck GW, Hoe HS
Biochimica et biophysica acta1853(5)904-17(2015 May)

Association between ZIC2, RASGRF1, and SHISA6 genes and high myopia in Japanese subjects.
Oishi M, Yamashiro K, Miyake M, Akagi-Kurashige Y, Kumagai K, Nakata I, Nakanishi H, Yoshikawa M, Oishi A, Gotoh N, Tsujikawa A, , Yamada R, Matsuda F, Yoshimura N
Investigative ophthalmology & visual science54(12)7492-7(2013 Nov)

Decreased expression of Ras-GRF1 in the brain tissue of the intractable epilepsy patients and experimental rats.
Zhu Q, Wang L, Xiao Z, Xiao F, Luo J, Zhang X, Peng X, Wang X, Sun H
Brain research149399-109(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rasgrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasgrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42708	NM_001105753.1 Latest version!	Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ORa42804	NM_001170531.1 Latest version!	Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasgrf1 ( NM_001105753.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099223.1 Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa42708
Clone ID Related Accession (Same CDS sequence)	NM_001105753.1
Accession Version	NM_001105753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CH473954.2. On or before Oct 3, 2007 this sequence version replaced XM_236468.4, XM_001063727.1, XM_001063783.1. Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a substantially shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAAG CCATCCGACT GAACGATGGC CACGTCGTGT CCCTGGGACT GCTGGCCCAG 
AGAGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTT CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAAAGTGA CTCGAGCTCT 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC AGTATCTGCA AACGCATGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGACAAA CAGCGTCATA GCAGGAGTGG ACGTCGACGT 
AATGTTCTGA GAAGACAACC AACCAGAATC TCAAAAACAT TCAGGCTGGT GGCAATTGAA 
TTGACTGGGA TGGCAGCCAC CCTTGAAGTC TCCTTCTCCA TAATTAGGAC AGAGATCTGT 
ACTGTTTCTC CAGCTCCACA AACAAGGATG GGAAACCCAG AAGACTTGTC AGCGCTGCGT 
GACAGAGTGG AGTCGAAGCG TCTCTTCCAC CATCCCTGTA GATTGATCAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099223.1
CDS	207..740
Translation	MQKAIRLNDGHVVSLGLLAQRDGTRKGYLSKRSSDNPKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGSICKRMPSPKRGTSSKESDKQRHSRSGRRRNVLRRQPTRISKTFRLVAIELTGMAATLEVSFSIIRTEICTVSPAPQTRMGNPEDLSALRDRVESKRLFHHPCRLII

Target ORF information:

RefSeq Version	NM_001105753.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105753.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGACT GAACGATGGC CACGTCGTGT CCCTGGGACT GCTGGCCCAG 
AGAGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTT CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAAAGTGA CTCGAGCTCT 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC AGTATCTGCA AACGCATGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGACAAA CAGCGTCATA GCAGGAGTGG ACGTCGACGT 
AATGTTCTGA GAAGACAACC AACCAGAATC TCAAAAACAT TCAGGCTGGT GGCAATTGAA 
TTGACTGGGA TGGCAGCCAC CCTTGAAGTC TCCTTCTCCA TAATTAGGAC AGAGATCTGT 
ACTGTTTCTC CAGCTCCACA AACAAGGATG GGAAACCCAG AAGACTTGTC AGCGCTGCGT 
GACAGAGTGG AGTCGAAGCG TCTCTTCCAC CATCCCTGTA GATTGATCAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( NM_001170531.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164002.1 Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa42804
Clone ID Related Accession (Same CDS sequence)	NM_001170531.1
Accession Version	NM_001170531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Rattus norvegicus(Norway rat)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB713048.1, CB607130.1, CB714920.1, BF405809.1, CB583610.1 and BC091317.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAAG CCATCCGACT GAACGATGGC CACGTCGTGT CCCTGGGACT GCTGGCCCAG 
AGAGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTT CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAAAGTGA CTCGAGCTCT 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC AGTATCTGCA AACGCATGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGACAAA CAGCATCATT ACTTCACAGT GAACTTCTCC 
AATGACAGCC AGAAGTCCCT AGAGCTGAGG ACCGATGACT CCAAGGACTG TGACGAGTGG 
GTGGCAGCGA TTGCTCGCGC CAGCTACAAG ATACTGGCCA CAGAGCATGA GGCGCTCATG 
CAGAAGTACC TGCACCTGCT GCAGGTGGTG GAGACAGAGA AGACCGTGGC TAAGCAGCTG 
CGACAGCAGC TCGAGGATGG CGAGGTCGAG ATCGAGCGCC TGAAGGCAGA GATTGCAAAC 
CTGATCAAGG ACAATGAACG TATCCAGTCC AACCAGCTGG TTGCCCCTGA GGATGAGGAC 
AGTGACATCA AGAAAATTAA GAAGGTACAG AGTTTCCTTC GCGGATGGCT GTGCCGGCGA 
AAGTGGAAGA ACATCATCCA GGACTACATC CGGTCTCCTC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAA GCTGAGGCCG AGTACGTGCA GCAACTACAC 
ATCCTTGTCA ACAATTTTCT GCGCCCACTG CGCATGGCCG CCAGCTCTAA GAAACCCCCT 
ATAACACATG ACGACGTCAG CAGTATCTTT CTGAACAGTG AGACCATCAT GTTCCTGCAC 
CAGATCTTCT ACCAAGGCCT GAAGGCCCGT ATCGCCAGCT GGCCCACCCT GGTTCTGGCG 
GACCTGTTCG ACATCCTGCT GCCAATGCTT AACATCTACC AGGAGTTCGT CCGCAACCAC 
CAGTACAGTC TCCAGATCCT AGCACACTGC AAGCAAAACC GGGACTTTGA CAAGCTCCTC 
AAGCAGTATG AGGCCAAGCC AGACTGCGAG GAGCGCACAC TGGAGACCTT CCTCACCTAT 
CCAATGTTCC AGATCCCCAG GTACATCCTG ACACTCCATG AGCTGCTGGC CCACACACCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TATGCCAAAT CCAAACTAGA GGAGCTGTCC 
AGGGTCATGC ACGACGAAGT CAGTGAGACC GAGAACATCC GCAAAAACCT GGCCATTGAG 
CGTATGATCA CCGAGGGCTG TGAGATCCTC CTTGACACCA GCCAGACCTT TGTGCGCCAA 
GGTTCCCTCA TCCAGGTGCC CATGTCAGAA AAGGGCAAGA TCAACAAGGG CCGCCTGGGG 
TCTCTGTCCC TTAAGAAAGA AGGTGAGCGC CAGTGTTTCC TGTTCTCCAA GCATCTCATC 
ATCTGCACCA GAGGCTCTGG TAGCAAACTG CACCTAACCA AGAATGGCGT GATTTCCCTC 
ATTGACTGCA CTCTACTGGA TGATCCAGAA AACATGGATG ATGACGGCAA AGGACAAGAG 
GTAGATCACC TGGACTTTAA GATTTGGGTG GAGCCAAAGG ATTCCCCACC CTTCACAGTC 
ATCCTGGTGG CCTCATCCAG GCAGGAGAAG GCGGCATGGA CCAGTGACAT CATCCAGTGC 
GTGGATAATA TCCGCTGCAA CGGGCTCATG ATGAATGCCT TTGAAGAAAA TTCCAAGGTC 
ACCGTGCCGC AGATGATCAA GTCTGATGCT TCCTTATACT GTGATGATGT TGACATTCGC 
TTCAGCAAAA CCATGAATTC TTGCAAAGTG CTGCAGATCC GCTATGCCAG CGTGGAGCGC 
CTGCTGGAGC GCCTGACTGA TCTTCGCTTC CTGAGTATTG ACTTTCTCAA CACCTTCCTG 
CACTCCTATC GAGTCTTCAC CGATGCTGTG GTGGTCCTAG ACAAGCTGAT CAGCATCTAC 
AAAAAGCCCA TCACTGCGAT TCCTGCCAGG TCACTGGAAC TCCTGTTCTC CAGTAGCCAC 
AACACCAAAC TTCTGTACGG AGATGCCCCC AAGTCGCCTC GTGCCAGCCG CAAGTTCTCC 
TCGCCGCCGC CCTTGGCCAT CGGCACTTCG TCCCCAGTCC GCCGCCGGAA GTTGTCTCTC 
AACATTCCCA TCATCACAGG CGGCAAGGCG CTGGAACTGG CTTCGCTCGG GTGCCCCTCC 
GACGGCTACA CCAACATACA CTCGCCCATA TCTCCCTTCG GCAAAACCAC GCTGGACACC 
AGCAAGCTCT GTGTGGCCAG CAGCTTGACC AGAACGCCGG AGGAGATTGA TATGACCACT 
CTAGAGGAGT CATCAGGCTT CAGGAAGCCG ACCTCAGACA TCTTGAAAGA AGAGTCTGAT 
GATGACCAGA GTGATGTAGA CGACACAGAA GTGTCTCCAC CAACACCGAA ATCATTCAGA 
AACAGAATCA CTCAAGAGTT CCCACTCTTT AACTACAACA GTGGAATCAT GATGACATGT 
CGCGATCTGA TGGACAGTAA CCGCAGCCCT CTGTCAGCTA CCTCTGCCTT TGCCATAGCG 
ACTGCAGGAG CCAATGAAAG CCCCGCAAAC AAGGAGATAT ATCGAAGGAT GTCTTTGGCC 
AACACAGGGT ATTCCTCTGA CCAGAGAAAT ATCGACAAAG AGTTCGTGAT CCGCAGAGCG 
GCCACCAACC GTGTACTGAA TGTGTTGCGC CACTGGGTCA CCAAGCACTC CCAGGACTTT 
GAAACTGACG ACCTCCTCAA ATACAAGGTG ATCTGCTTTC TGGAAGAGGT CATGCATGAC 
CCAGACCTTC TACCACAAGA GCGAAAGGCA GCAGCCAACA TCATGAGGAC TCTGACCCAG 
GAAGAAATAA CTGAAAACCA TAGCATGCTG GATGAGCTCT TACTAATGAC GGAGGGTGTG 
AAGACTGAGC CCTTCGAAAA CCACTCAGCC ATGGAGATAG CAGAGCAGCT GACCCTGCTG 
GATCACCTTG TCTTCAAGAG TATTCCTTAT GAGGAATTCT TTGGCCAGGG CTGGATGAAG 
GCAGATAAGA ATGAAAGGAC ACCTTACATT ATGAAAACCA CCAGACATTT CAACCATATC 
AGTAACTTGA TCGCTTCAGA AATTCTCCGA AACGAGGAGG TCAGTGCAAG GGCAAGCACC 
ATCGAGAAGT GGGTGGCTGT TGCCGACATT TGCCGCTGCC TGCACAACTA CAATGCTGTG 
CTGGAGATCA CTTCCTCCAT CAACCGCAGC GCAATCTTCC GACTCAAGAA GACATGGCTC 
AAAGTTTCTA AGCAGACGAA ATCTCTGTTT GACAAGCTCC AAAAGCTTGT GTCATCAGAT 
GGCCGATTTA AGAACCTCAG AGAAACTTTG CGAAATTGTG ATCCACCCTG TGTCCCTTAC 
CTGGGGATGT ACCTGACCGA CTTGGCATTC CTCGAGGAAG GAACACCCAA TTACACAGAG 
GACGGCCTGG TCAACTTCTC CAAGATGAGG ATGATCTCCC ATATTATCCG CGAGATTCGC 
CAGTTTCAGC AGACTACTTA CAAAATCGAG CCCCAGCCAA AGGTAACTCA GTACTTAGTG 
GATGAAACCT TTGTGTTGGA CGACGAAAGT CTGTATGAGG CCTCCCTCCG AATTGAACCA 
AAACTCCCCA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164002.1
CDS	131..3865
Translation	MQKAIRLNDGHVVSLGLLAQRDGTRKGYLSKRSSDNPKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGSICKRMPSPKRGTSSKESDKQHHYFTVNFSNDSQKSLELRTDDSKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKAEIANLIKDNERIQSNQLVAPEDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRVMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLIQVPMSEKGKINKGRLGSLSLKKEGERQCFLFSKHLIICTRGSGSKLHLTKNGVISLIDCTLLDDPENMDDDGKGQEVDHLDFKIWVEPKDSPPFTVILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTDAVVVLDKLISIYKKPITAIPARSLELLFSSSHNTKLLYGDAPKSPRASRKFSSPPPLAIGTSSPVRRRKLSLNIPIITGGKALELASLGCPSDGYTNIHSPISPFGKTTLDTSKLCVASSLTRTPEEIDMTTLEESSGFRKPTSDILKEESDDDQSDVDDTEVSPPTPKSFRNRITQEFPLFNYNSGIMMTCRDLMDSNRSPLSATSAFAIATAGANESPANKEIYRRMSLANTGYSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHSQDFETDDLLKYKVICFLEEVMHDPDLLPQERKAAANIMRTLTQEEITENHSMLDELLLMTEGVKTEPFENHSAMEIAEQLTLLDHLVFKSIPYEEFFGQGWMKADKNERTPYIMKTTRHFNHISNLIASEILRNEEVSARASTIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLFDKLQKLVSSDGRFKNLRETLRNCDPPCVPYLGMYLTDLAFLEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIEPQPKVTQYLVDETFVLDDESLYEASLRIEPKLPT

Target ORF information:

RefSeq Version	NM_001170531.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170531.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGACT GAACGATGGC CACGTCGTGT CCCTGGGACT GCTGGCCCAG 
AGAGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTT CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAAAGTGA CTCGAGCTCT 
CGGCCCTCGG GGCTCTACCT GCTGGAGGGC AGTATCTGCA AACGCATGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGACAAA CAGCATCATT ACTTCACAGT GAACTTCTCC 
AATGACAGCC AGAAGTCCCT AGAGCTGAGG ACCGATGACT CCAAGGACTG TGACGAGTGG 
GTGGCAGCGA TTGCTCGCGC CAGCTACAAG ATACTGGCCA CAGAGCATGA GGCGCTCATG 
CAGAAGTACC TGCACCTGCT GCAGGTGGTG GAGACAGAGA AGACCGTGGC TAAGCAGCTG 
CGACAGCAGC TCGAGGATGG CGAGGTCGAG ATCGAGCGCC TGAAGGCAGA GATTGCAAAC 
CTGATCAAGG ACAATGAACG TATCCAGTCC AACCAGCTGG TTGCCCCTGA GGATGAGGAC 
AGTGACATCA AGAAAATTAA GAAGGTACAG AGTTTCCTTC GCGGATGGCT GTGCCGGCGA 
AAGTGGAAGA ACATCATCCA GGACTACATC CGGTCTCCTC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAA GCTGAGGCCG AGTACGTGCA GCAACTACAC 
ATCCTTGTCA ACAATTTTCT GCGCCCACTG CGCATGGCCG CCAGCTCTAA GAAACCCCCT 
ATAACACATG ACGACGTCAG CAGTATCTTT CTGAACAGTG AGACCATCAT GTTCCTGCAC 
CAGATCTTCT ACCAAGGCCT GAAGGCCCGT ATCGCCAGCT GGCCCACCCT GGTTCTGGCG 
GACCTGTTCG ACATCCTGCT GCCAATGCTT AACATCTACC AGGAGTTCGT CCGCAACCAC 
CAGTACAGTC TCCAGATCCT AGCACACTGC AAGCAAAACC GGGACTTTGA CAAGCTCCTC 
AAGCAGTATG AGGCCAAGCC AGACTGCGAG GAGCGCACAC TGGAGACCTT CCTCACCTAT 
CCAATGTTCC AGATCCCCAG GTACATCCTG ACACTCCATG AGCTGCTGGC CCACACACCT 
CATGAGCATG TGGAGCGCAA CAGCCTGGAC TATGCCAAAT CCAAACTAGA GGAGCTGTCC 
AGGGTCATGC ACGACGAAGT CAGTGAGACC GAGAACATCC GCAAAAACCT GGCCATTGAG 
CGTATGATCA CCGAGGGCTG TGAGATCCTC CTTGACACCA GCCAGACCTT TGTGCGCCAA 
GGTTCCCTCA TCCAGGTGCC CATGTCAGAA AAGGGCAAGA TCAACAAGGG CCGCCTGGGG 
TCTCTGTCCC TTAAGAAAGA AGGTGAGCGC CAGTGTTTCC TGTTCTCCAA GCATCTCATC 
ATCTGCACCA GAGGCTCTGG TAGCAAACTG CACCTAACCA AGAATGGCGT GATTTCCCTC 
ATTGACTGCA CTCTACTGGA TGATCCAGAA AACATGGATG ATGACGGCAA AGGACAAGAG 
GTAGATCACC TGGACTTTAA GATTTGGGTG GAGCCAAAGG ATTCCCCACC CTTCACAGTC 
ATCCTGGTGG CCTCATCCAG GCAGGAGAAG GCGGCATGGA CCAGTGACAT CATCCAGTGC 
GTGGATAATA TCCGCTGCAA CGGGCTCATG ATGAATGCCT TTGAAGAAAA TTCCAAGGTC 
ACCGTGCCGC AGATGATCAA GTCTGATGCT TCCTTATACT GTGATGATGT TGACATTCGC 
TTCAGCAAAA CCATGAATTC TTGCAAAGTG CTGCAGATCC GCTATGCCAG CGTGGAGCGC 
CTGCTGGAGC GCCTGACTGA TCTTCGCTTC CTGAGTATTG ACTTTCTCAA CACCTTCCTG 
CACTCCTATC GAGTCTTCAC CGATGCTGTG GTGGTCCTAG ACAAGCTGAT CAGCATCTAC 
AAAAAGCCCA TCACTGCGAT TCCTGCCAGG TCACTGGAAC TCCTGTTCTC CAGTAGCCAC 
AACACCAAAC TTCTGTACGG AGATGCCCCC AAGTCGCCTC GTGCCAGCCG CAAGTTCTCC 
TCGCCGCCGC CCTTGGCCAT CGGCACTTCG TCCCCAGTCC GCCGCCGGAA GTTGTCTCTC 
AACATTCCCA TCATCACAGG CGGCAAGGCG CTGGAACTGG CTTCGCTCGG GTGCCCCTCC 
GACGGCTACA CCAACATACA CTCGCCCATA TCTCCCTTCG GCAAAACCAC GCTGGACACC 
AGCAAGCTCT GTGTGGCCAG CAGCTTGACC AGAACGCCGG AGGAGATTGA TATGACCACT 
CTAGAGGAGT CATCAGGCTT CAGGAAGCCG ACCTCAGACA TCTTGAAAGA AGAGTCTGAT 
GATGACCAGA GTGATGTAGA CGACACAGAA GTGTCTCCAC CAACACCGAA ATCATTCAGA 
AACAGAATCA CTCAAGAGTT CCCACTCTTT AACTACAACA GTGGAATCAT GATGACATGT 
CGCGATCTGA TGGACAGTAA CCGCAGCCCT CTGTCAGCTA CCTCTGCCTT TGCCATAGCG 
ACTGCAGGAG CCAATGAAAG CCCCGCAAAC AAGGAGATAT ATCGAAGGAT GTCTTTGGCC 
AACACAGGGT ATTCCTCTGA CCAGAGAAAT ATCGACAAAG AGTTCGTGAT CCGCAGAGCG 
GCCACCAACC GTGTACTGAA TGTGTTGCGC CACTGGGTCA CCAAGCACTC CCAGGACTTT 
GAAACTGACG ACCTCCTCAA ATACAAGGTG ATCTGCTTTC TGGAAGAGGT CATGCATGAC 
CCAGACCTTC TACCACAAGA GCGAAAGGCA GCAGCCAACA TCATGAGGAC TCTGACCCAG 
GAAGAAATAA CTGAAAACCA TAGCATGCTG GATGAGCTCT TACTAATGAC GGAGGGTGTG 
AAGACTGAGC CCTTCGAAAA CCACTCAGCC ATGGAGATAG CAGAGCAGCT GACCCTGCTG 
GATCACCTTG TCTTCAAGAG TATTCCTTAT GAGGAATTCT TTGGCCAGGG CTGGATGAAG 
GCAGATAAGA ATGAAAGGAC ACCTTACATT ATGAAAACCA CCAGACATTT CAACCATATC 
AGTAACTTGA TCGCTTCAGA AATTCTCCGA AACGAGGAGG TCAGTGCAAG GGCAAGCACC 
ATCGAGAAGT GGGTGGCTGT TGCCGACATT TGCCGCTGCC TGCACAACTA CAATGCTGTG 
CTGGAGATCA CTTCCTCCAT CAACCGCAGC GCAATCTTCC GACTCAAGAA GACATGGCTC 
AAAGTTTCTA AGCAGACGAA ATCTCTGTTT GACAAGCTCC AAAAGCTTGT GTCATCAGAT 
GGCCGATTTA AGAACCTCAG AGAAACTTTG CGAAATTGTG ATCCACCCTG TGTCCCTTAC 
CTGGGGATGT ACCTGACCGA CTTGGCATTC CTCGAGGAAG GAACACCCAA TTACACAGAG 
GACGGCCTGG TCAACTTCTC CAAGATGAGG ATGATCTCCC ATATTATCCG CGAGATTCGC 
CAGTTTCAGC AGACTACTTA CAAAATCGAG CCCCAGCCAA AGGTAACTCA GTACTTAGTG 
GATGAAACCT TTGTGTTGGA CGACGAAAGT CTGTATGAGG CCTCCCTCCG AATTGAACCA 
AAACTCCCCA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.
Arteriosclerosis, thrombosis, and vascular biology37(1)98-107(2017 01)
Wu RF,Liao C,Hatoum H,Fu G,Ochoa CD,Terada LS

A Mercaptoacetamide-Based Class II Histone Deacetylase Inhibitor Increases Dendritic Spine Density via RasGRF1/ERK Pathway.
Journal of Alzheimer's disease : JAD51(2)591-604(2016)
Song JM,Sung YM,Nam JH,Yoon H,Chung A,Moffat E,Jung M,Pak DT,Kim J,Hoe HS

Very low density lipoprotein receptor regulates dendritic spine formation in a RasGRF1/CaMKII dependent manner.
Biochimica et biophysica acta1853(5)904-17(2015 May)
DiBattista AM,Dumanis SB,Song JM,Bu G,Weeber E,Rebeck GW,Hoe HS

Association between ZIC2, RASGRF1, and SHISA6 genes and high myopia in Japanese subjects.
Investigative ophthalmology & visual science54(12)7492-7(2013 Nov)
Oishi M,Yamashiro K,Miyake M,Akagi-Kurashige Y,Kumagai K,Nakata I,Nakanishi H,Yoshikawa M,Oishi A,Gotoh N,Tsujikawa A,,Yamada R,Matsuda F,Yoshimura N

Decreased expression of Ras-GRF1 in the brain tissue of the intractable epilepsy patients and experimental rats.
Brain research149399-109(2013 Feb)
Zhu Q,Wang L,Xiao Z,Xiao F,Luo J,Zhang X,Peng X,Wang X,Sun H

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