eif2ak1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	eif2ak1
Entrez Gene ID	565338
Full Name	eukaryotic translation initiation factor 2-alpha kinase 1
Synonyms	wu:fj97h09,zgc:153232
General protein information	Preferred Names eukaryotic translation initiation factor 2-alpha kinase 1 Names eukaryotic translation initiation factor 2-alpha kinase 1 fj97h09
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169457.3	XP_005169514.1	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
NM_001077567.1	NP_001071035.1	eukaryotic translation initiation factor 2-alpha kinase 1
XM_021480133.1	XP_021335808.1	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
XM_021480134.1	XP_021335809.1	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	hri2	NP_593146.1
Macaca mulatta (Rhesus monkey)	EIF2AK1	XP_001089983.2
Rattus norvegicus (Norway rat)	Eif2ak1	NP_037355.1
Danio rerio (zebrafish)	eif2ak1	NP_001071035.1
Canis lupus familiaris (dog)	EIF2AK1	NP_001155185.1
Gallus gallus (chicken)	EIF2AK1	NP_989979.1
Pan troglodytes (chimpanzee)	EIF2AK1	XP_518962.3
Homo sapiens (human)	EIF2AK1	NP_001127807.1
Mus musculus (house mouse)	Eif2ak1	NP_038585.2
Xenopus tropicalis (tropical clawed frog)	eif2ak1	NP_001039038.1
Schizosaccharomyces pombe (fission yeast)	hri1	NP_594738.1
Bos taurus (cattle)	EIF2AK1	NP_001033142.1

Gene Ontology
Bibliography

Related articles in PubMed

Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos.
Tsai TC, Shih CC, Chien HP, Yang AH, Lu JK, Lu JH
BMC developmental biology18(1)5(2018 03)

A truncated Danio rerio PKZ isoform functionally interacts with eIF2α and inhibits protein synthesis.
Liu ZY, Jia KT, Li C, Weng SP, Guo CJ, He JG
Gene527(1)292-300(2013 Sep)

A shifted repertoire of endocannabinoid genes in the zebrafish (Danio rerio).
McPartland JM, Glass M, Matias I, Norris RW, Kilpatrick CW
Molecular genetics and genomics : MGG277(5)555-70(2007 May)

A PKR-like eukaryotic initiation factor 2alpha kinase from zebrafish contains Z-DNA binding domains instead of dsRNA binding domains.
Rothenburg S, Deigendesch N, Dittmar K, Koch-Nolte F, Haag F, Lowenhaupt K, Rich A
Proceedings of the National Academy of Sciences of the United States of America102(5)1602-7(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eif2ak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif2ak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36404	NM_001077567.1 Latest version!	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa17749	XM_021480133.1 Latest version!	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa17749	XM_005169457.3 Latest version!	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa17750	XM_021480134.1 Latest version!	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eif2ak1 ( NM_001077567.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001071035.1 Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), mRNA.

Price & Availability↑

CloneID	ODa36404
Clone ID Related Accession (Same CDS sequence)	NM_001077567.1
Accession Version	NM_001077567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556553600000
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation initiation factor 2-alpha kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124654.1. On Nov 9, 2006 this sequence version replaced XM_688615.1. ##Evidence-Data-START## Transcript exon combination :: BC124654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTCAGCC TGACAGATAC GGAGGACGAC GTCCAATTTG ACACCTCGGA TTCAGACACT 
AACTGTGAGG TGTTGCTGGC CGGAAAACAG TATCCATCGA TTCAGGAATT TGCATCTGCC 
ATTCCCAACC AACTTCTGCT TGGATCTCTA CTTGAACACT TGTGCTTTGT TTATGAGAGT 
GATCCCACAC GGTCACGCAT GCTGTTTAAA GTGATAGGCC AGCGGTTAGC GGCTATGAAC 
CTCCTGTCCC CACTGGCGAT CAGTGATGAG TTCAGCACGG TCCGATTACA GCATAACAGG 
GCATTCACAG AGCTCCTGCA CGCCACAAGC TCATCCCTTT TCCCCCAGGA ACATAAGTAC 
TTGAGTACAG AAGGCCAAAG CAAAAGAACC AAAGATGGTC TTTTCCAAGC ACAGACATCC 
CGGTACCTTA GTGAATTTGA AGAAATAGCA ACACTGGGTA AAGGATCCTA TGGCAAAGTG 
TTTAGGGTCA CAAACAAGCT TGATGGTCAG GAATATGCAG TGAAAAAAAT TCTAATCAAG 
AAAGTAACAC GAGATGACTG TATGAAGGTT CTTAGAGAGG TAAAAGTGCT CTCCAGCCTT 
CAGCATCCCG ATATTGTTGG ATATCACACT GCATGGATGG AACATGTTCA GCCTGCTGTG 
ACGGTAGATT CCAACTCTCT TGTTTCGCAA ACATTTCCAG CATTAGATAG ACCTTCAGAA 
CCAGATGGGT GCACTTCAGA ACACACCAAC AGCAGCAATG GATCTTTCAT TGTTTTTGAA 
CATTCTGAAC AGGCAGAAGT GAAAGAAAAT CGGGTTGCAC TAGAGAGCAT CTCGGTAAAA 
CCCTCAACTA CAAAAGTGGA CATGGCACAG TCTGTTTGCC CTAAAAATGT CCGCAAGCCA 
CAGAATTTTG TTCCATGTGT GTTTCTGGGA AAACCCCGCT CTACGTTAAA CAAATGCCCT 
GCTGCTAATT GGGACAGTTC AACACTCTCA GAGGATGACC TCAGTCAGAA CAGACTGGAA 
TTGAACAAAC ACTCTTACAT TGACATGGAC ACACCCGAAT GGTCTGAAAA GCAGGAGAAC 
AAGGTTCAGT TCCACTTAAT GCTTTATATA CAAATGCAGC TGTGTGAACG ATCTTTGAAG 
GACTGGATAC AAGAGAGAAA TTCAAGGACC ACGGAGGGAC TCTTGATGTC AGGAGATCCC 
TGTGATTCAG TTGACTGTGA ACAGGCATTA AAGATTTTAA AGAAAACACT TGAAGGAGTG 
GAGTACATTC ATTCCTGTGG TATTATGCAC AGAGACCTAA AGCCAAGAAA TATTTTCCTT 
CTTGGAAATG AGTGCCATGT GAAGATAGGG GATTTTGGAT TGGCTTGCCA AAACATTATA 
ATGGACGAAC ATGAGAAGTT GCCTTCCAGT TCCCAAGCAG GTGTAAACCC AGATGCAACT 
CACACCAGTG GAGTGGGAAC ATTTGTGTAC GCTGCCCCAG AACAACTTGA GGGCTCTCGC 
TATGATTCAA AGTCAGATAT GTACAGCATA GGAGTGATTG CCTTTGAGTT ATTCCAGCCA 
TTTGGGACCG AGATGGAGCG TGTTCACACA CTTGGAGAAC TACGACAGGG CAAGATCCCT 
AACACACTGT CCACAAACTG GCCAATTTTG GCCAAGTATA TTAAACTACT GACTTCCTCT 
GATCCCTCAA TGCGACCAAG TGCACCTCAG TTACTTCAGA GTGACCTCTT CAGCACCAAT 
GACATGGTGA TCCACAGTTT AAAGCGCAAG ATTGATGAAC AAGAAGAAGA AATTGTACAG 
CTCAGGAGAC GGATTAGTGA GCTACAGATC TCTCAGAATT CAATACACAG TCCAGAAAAG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071035.1
CDS	331..2196
Translation	MFSLTDTEDDVQFDTSDSDTNCEVLLAGKQYPSIQEFASAIPNQLLLGSLLEHLCFVYESDPTRSRMLFKVIGQRLAAMNLLSPLAISDEFSTVRLQHNRAFTELLHATSSSLFPQEHKYLSTEGQSKRTKDGLFQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIKKVTRDDCMKVLREVKVLSSLQHPDIVGYHTAWMEHVQPAVTVDSNSLVSQTFPALDRPSEPDGCTSEHTNSSNGSFIVFEHSEQAEVKENRVALESISVKPSTTKVDMAQSVCPKNVRKPQNFVPCVFLGKPRSTLNKCPAANWDSSTLSEDDLSQNRLELNKHSYIDMDTPEWSEKQENKVQFHLMLYIQMQLCERSLKDWIQERNSRTTEGLLMSGDPCDSVDCEQALKILKKTLEGVEYIHSCGIMHRDLKPRNIFLLGNECHVKIGDFGLACQNIIMDEHEKLPSSSQAGVNPDATHTSGVGTFVYAAPEQLEGSRYDSKSDMYSIGVIAFELFQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTSSDPSMRPSAPQLLQSDLFSTNDMVIHSLKRKIDEQEEEIVQLRRRISELQISQNSIHSPEKT

Target ORF information:

RefSeq Version	NM_001077567.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077567.1

ORF Insert Sequence:

ATGTTCAGCC TGACAGATAC GGAGGACGAC GTCCAATTTG ACACCTCGGA TTCAGACACT 
AACTGTGAGG TGTTGCTGGC CGGAAAACAG TATCCATCGA TTCAGGAATT TGCATCTGCC 
ATTCCCAACC AACTTCTGCT TGGATCTCTA CTTGAACACT TGTGCTTTGT TTATGAGAGT 
GATCCCACAC GGTCACGCAT GCTGTTTAAA GTGATAGGCC AGCGGTTAGC GGCTATGAAC 
CTCCTGTCCC CACTGGCGAT CAGTGATGAG TTCAGCACGG TCCGATTACA GCATAACAGG 
GCATTCACAG AGCTCCTGCA CGCCACAAGC TCATCCCTTT TCCCCCAGGA ACATAAGTAC 
TTGAGTACAG AAGGCCAAAG CAAAAGAACC AAAGATGGTC TTTTCCAAGC ACAGACATCC 
CGGTACCTTA GTGAATTTGA AGAAATAGCA ACACTGGGTA AAGGATCCTA TGGCAAAGTG 
TTTAGGGTCA CAAACAAGCT TGATGGTCAG GAATATGCAG TGAAAAAAAT TCTAATCAAG 
AAAGTAACAC GAGATGACTG TATGAAGGTT CTTAGAGAGG TAAAAGTGCT CTCCAGCCTT 
CAGCATCCCG ATATTGTTGG ATATCACACT GCATGGATGG AACATGTTCA GCCTGCTGTG 
ACGGTAGATT CCAACTCTCT TGTTTCGCAA ACATTTCCAG CATTAGATAG ACCTTCAGAA 
CCAGATGGGT GCACTTCAGA ACACACCAAC AGCAGCAATG GATCTTTCAT TGTTTTTGAA 
CATTCTGAAC AGGCAGAAGT GAAAGAAAAT CGGGTTGCAC TAGAGAGCAT CTCGGTAAAA 
CCCTCAACTA CAAAAGTGGA CATGGCACAG TCTGTTTGCC CTAAAAATGT CCGCAAGCCA 
CAGAATTTTG TTCCATGTGT GTTTCTGGGA AAACCCCGCT CTACGTTAAA CAAATGCCCT 
GCTGCTAATT GGGACAGTTC AACACTCTCA GAGGATGACC TCAGTCAGAA CAGACTGGAA 
TTGAACAAAC ACTCTTACAT TGACATGGAC ACACCCGAAT GGTCTGAAAA GCAGGAGAAC 
AAGGTTCAGT TCCACTTAAT GCTTTATATA CAAATGCAGC TGTGTGAACG ATCTTTGAAG 
GACTGGATAC AAGAGAGAAA TTCAAGGACC ACGGAGGGAC TCTTGATGTC AGGAGATCCC 
TGTGATTCAG TTGACTGTGA ACAGGCATTA AAGATTTTAA AGAAAACACT TGAAGGAGTG 
GAGTACATTC ATTCCTGTGG TATTATGCAC AGAGACCTAA AGCCAAGAAA TATTTTCCTT 
CTTGGAAATG AGTGCCATGT GAAGATAGGG GATTTTGGAT TGGCTTGCCA AAACATTATA 
ATGGACGAAC ATGAGAAGTT GCCTTCCAGT TCCCAAGCAG GTGTAAACCC AGATGCAACT 
CACACCAGTG GAGTGGGAAC ATTTGTGTAC GCTGCCCCAG AACAACTTGA GGGCTCTCGC 
TATGATTCAA AGTCAGATAT GTACAGCATA GGAGTGATTG CCTTTGAGTT ATTCCAGCCA 
TTTGGGACCG AGATGGAGCG TGTTCACACA CTTGGAGAAC TACGACAGGG CAAGATCCCT 
AACACACTGT CCACAAACTG GCCAATTTTG GCCAAGTATA TTAAACTACT GACTTCCTCT 
GATCCCTCAA TGCGACCAAG TGCACCTCAG TTACTTCAGA GTGACCTCTT CAGCACCAAT 
GACATGGTGA TCCACAGTTT AAAGCGCAAG ATTGATGAAC AAGAAGAAGA AATTGTACAG 
CTCAGGAGAC GGATTAGTGA GCTACAGATC TCTCAGAATT CAATACACAG TCCAGAAAAG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak1 ( XM_005169457.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169514.1 Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17749
Clone ID Related Accession (Same CDS sequence)	XM_005169457.3 , XM_021480133.1
Accession Version	XM_005169457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCACA GAAAAGAGAC CCTCATGGTA GTCGAAAGTT CGAGTAACGT TAGCAGTGTA 
GTTTCCAATG TTAACAGCCG TAGCCTTCAT TTACAACGGG GCCTTTCCAC CCAAGAGAGA 
GAATCTAACA TTAGAGGGAA AATGTTCAGC CTGACAGATA CGGAGGACGA CGTCCAATTT 
GACACCTCGG ATTCAGACAC TAACTGTGAG GTGTTGCTGG CCGGAAAACA GTATCCATCG 
ATTCAGGAAT TTGCATCTGC CATTCCCAAC CAACTTCTGC TTGGATCTCT ACTTGAACAC 
TTGTGCTTTG TTTATGAGAG TGATCCCACA CGGTCACGCA TGCTGTTTAA AGTGATAGGC 
CAGCGGTTAG CGGCTATGAA CCTCCTGTCC CCACTGGCGA TCAGTGATGA GTTCAGCACG 
GTCCGATTAC AGCATAACAG GGCATTCACA GAGCTCCTGC ACGCCACAAG CTCATCCCTT 
TTCCCCCAGG AACATAAGTA CTTGAGTACA GAAGGCCAAA GCAAAAGAAC CAAAGATGGT 
CTTTTCCAAG CACAGACATC CCGGTACCTT AGTGAATTTG AAGAAATAGC AACACTGGGT 
AAAGGATCCT ATGGCAAAGT GTTTAGGGTC ACAAACAAGC TTGATGGTCA GGAATATGCA 
GTGAAAAAAA TTCTAATCAA GAAAGTAACA CGAGATGACT GTATGAAGGT TCTTAGAGAG 
GTTAAAGTGC TCTCCAGCCT TCAGCATCCC AATATTGTTG GATATCACAC TGCATGGATG 
GAACACGTTC AGCCTGCTGT GACGGATTCC AACTCTCTTG TTTCGCAAAC ATTTCCAGCA 
TTAGATAGAC CTTCAGAACC AAATGGGTGC ACTCCAGAAC ACACCAACAG CAGCAATGGA 
TCTTTCATTG TTTTTGAACA TTCTGAACGG GCAGAAGTGA AAGAAAATCA GGTTGCACTA 
GAGAGCATCT CGGTAAACCC CTCAACTTCA AAAGTGGACA TGGCACAGTC TGTTTGCCCT 
AAAAATGTCC GCAAGCCACA GAATTTTGTT CCATGTGTGT TTCTGGGAAA ACCCCGCTCT 
ACGTTAAACA AATGCCCTGC TGCTAATTGG GACAGTTCAA CACTCTCAGA GGATGACCTC 
AGTCAGAACA GACTGGAATT GAACAAACAC TCTTACATTG ACATGGACAC ACCCGAATGG 
TCTGAAAAGC AGGAGAACAA GGTTCAGTTC CACTTAATGC TTTATATACA AATGCAGCTG 
TGTGAACGAT CTTTGAAGGA CTGGATACAA GAGAGAAATT CAAGGACCAC GGAGGGACTC 
TTGATGTCAG GAGATCCCTG TGATTCAGTT GACTGTGAAC AGGCATTAAA GATTTTAAAG 
AAAATACTTG AAGGAGTGGA GTACATTCAT TCCTGTGGTA TTATGCACAG AGACCTAAAG 
CCAAGAAATA TTTTCCTTCT TGGAAATGAG TGCCATGTGA AGATAGGGGA TTTTGGATTG 
GCTTGCCAAA ACATTATAAT GGACGAACAT GAGAAGTTGC CTTCCAGTTC CCAAGCAGGT 
GTAAACCCAG ATGCAACTCA CACCAGTGGA GTGGGAACAT TTGTGTACGC TGCCCCAGAA 
CAACTTGAGG GCTCTCGCTA TGATTCAAAG TCAGATATGT ACAGCATAGG AGTGATTGCC 
TTTGAGTTAT TCCAGCCATT TGGGACCGAG ATGGAGCGTG TTCACACACT TGGAGAACTA 
CGACAGGGCA AGATCCCTAA CACACTGTCC ACAAACTGGC CAATTTTGGC CAAGTATATT 
AAACTACTGA CCTCCTCTGA TCCCTCAATG CGACCAAGTG CACCTCAGTT ACTTCAGAGT 
GACCTCTTCA GCACCAATGA CATGGTGATC CACAGTTTAA AGCGCAAGAT TGATGAACAA 
GAAGAAGAAA TTGTACAGCT CAGGAGACGG ATTAGTGAGC TACAGATCTC TCAGAATTCA 
ATACACAGTC CAGAAAAGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169514.1
CDS	169..2172
Translation	MFHRKETLMVVESSSNVSSVVSNVNSRSLHLQRGLSTQERESNIRGKMFSLTDTEDDVQFDTSDSDTNCEVLLAGKQYPSIQEFASAIPNQLLLGSLLEHLCFVYESDPTRSRMLFKVIGQRLAAMNLLSPLAISDEFSTVRLQHNRAFTELLHATSSSLFPQEHKYLSTEGQSKRTKDGLFQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIKKVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVTDSNSLVSQTFPALDRPSEPNGCTPEHTNSSNGSFIVFEHSERAEVKENQVALESISVNPSTSKVDMAQSVCPKNVRKPQNFVPCVFLGKPRSTLNKCPAANWDSSTLSEDDLSQNRLELNKHSYIDMDTPEWSEKQENKVQFHLMLYIQMQLCERSLKDWIQERNSRTTEGLLMSGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLLGNECHVKIGDFGLACQNIIMDEHEKLPSSSQAGVNPDATHTSGVGTFVYAAPEQLEGSRYDSKSDMYSIGVIAFELFQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTSSDPSMRPSAPQLLQSDLFSTNDMVIHSLKRKIDEQEEEIVQLRRRISELQISQNSIHSPEKT

Target ORF information:

RefSeq Version	XM_005169457.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169457.3

ORF Insert Sequence:

ATGTTTCACA GAAAAGAGAC CCTCATGGTA GTCGAAAGTT CGAGTAACGT TAGCAGTGTA 
GTTTCCAATG TTAACAGCCG TAGCCTTCAT TTACAACGGG GCCTTTCCAC CCAAGAGAGA 
GAATCTAACA TTAGAGGGAA AATGTTCAGC CTGACAGATA CGGAGGACGA CGTCCAATTT 
GACACCTCGG ATTCAGACAC TAACTGTGAG GTGTTGCTGG CCGGAAAACA GTATCCATCG 
ATTCAGGAAT TTGCATCTGC CATTCCCAAC CAACTTCTGC TTGGATCTCT ACTTGAACAC 
TTGTGCTTTG TTTATGAGAG TGATCCCACA CGGTCACGCA TGCTGTTTAA AGTGATAGGC 
CAGCGGTTAG CGGCTATGAA CCTCCTGTCC CCACTGGCGA TCAGTGATGA GTTCAGCACG 
GTCCGATTAC AGCATAACAG GGCATTCACA GAGCTCCTGC ACGCCACAAG CTCATCCCTT 
TTCCCCCAGG AACATAAGTA CTTGAGTACA GAAGGCCAAA GCAAAAGAAC CAAAGATGGT 
CTTTTCCAAG CACAGACATC CCGGTACCTT AGTGAATTTG AAGAAATAGC AACACTGGGT 
AAAGGATCCT ATGGCAAAGT GTTTAGGGTC ACAAACAAGC TTGATGGTCA GGAATATGCA 
GTGAAAAAAA TTCTAATCAA GAAAGTAACA CGAGATGACT GTATGAAGGT TCTTAGAGAG 
GTTAAAGTGC TCTCCAGCCT TCAGCATCCC AATATTGTTG GATATCACAC TGCATGGATG 
GAACACGTTC AGCCTGCTGT GACGGATTCC AACTCTCTTG TTTCGCAAAC ATTTCCAGCA 
TTAGATAGAC CTTCAGAACC AAATGGGTGC ACTCCAGAAC ACACCAACAG CAGCAATGGA 
TCTTTCATTG TTTTTGAACA TTCTGAACGG GCAGAAGTGA AAGAAAATCA GGTTGCACTA 
GAGAGCATCT CGGTAAACCC CTCAACTTCA AAAGTGGACA TGGCACAGTC TGTTTGCCCT 
AAAAATGTCC GCAAGCCACA GAATTTTGTT CCATGTGTGT TTCTGGGAAA ACCCCGCTCT 
ACGTTAAACA AATGCCCTGC TGCTAATTGG GACAGTTCAA CACTCTCAGA GGATGACCTC 
AGTCAGAACA GACTGGAATT GAACAAACAC TCTTACATTG ACATGGACAC ACCCGAATGG 
TCTGAAAAGC AGGAGAACAA GGTTCAGTTC CACTTAATGC TTTATATACA AATGCAGCTG 
TGTGAACGAT CTTTGAAGGA CTGGATACAA GAGAGAAATT CAAGGACCAC GGAGGGACTC 
TTGATGTCAG GAGATCCCTG TGATTCAGTT GACTGTGAAC AGGCATTAAA GATTTTAAAG 
AAAATACTTG AAGGAGTGGA GTACATTCAT TCCTGTGGTA TTATGCACAG AGACCTAAAG 
CCAAGAAATA TTTTCCTTCT TGGAAATGAG TGCCATGTGA AGATAGGGGA TTTTGGATTG 
GCTTGCCAAA ACATTATAAT GGACGAACAT GAGAAGTTGC CTTCCAGTTC CCAAGCAGGT 
GTAAACCCAG ATGCAACTCA CACCAGTGGA GTGGGAACAT TTGTGTACGC TGCCCCAGAA 
CAACTTGAGG GCTCTCGCTA TGATTCAAAG TCAGATATGT ACAGCATAGG AGTGATTGCC 
TTTGAGTTAT TCCAGCCATT TGGGACCGAG ATGGAGCGTG TTCACACACT TGGAGAACTA 
CGACAGGGCA AGATCCCTAA CACACTGTCC ACAAACTGGC CAATTTTGGC CAAGTATATT 
AAACTACTGA CCTCCTCTGA TCCCTCAATG CGACCAAGTG CACCTCAGTT ACTTCAGAGT 
GACCTCTTCA GCACCAATGA CATGGTGATC CACAGTTTAA AGCGCAAGAT TGATGAACAA 
GAAGAAGAAA TTGTACAGCT CAGGAGACGG ATTAGTGAGC TACAGATCTC TCAGAATTCA 
ATACACAGTC CAGAAAAGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak1 ( XM_021480133.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335808.1 Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17749
Clone ID Related Accession (Same CDS sequence)	XM_005169457.3 , XM_021480133.1
Accession Version	XM_021480133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCACA GAAAAGAGAC CCTCATGGTA GTCGAAAGTT CGAGTAACGT TAGCAGTGTA 
GTTTCCAATG TTAACAGCCG TAGCCTTCAT TTACAACGGG GCCTTTCCAC CCAAGAGAGA 
GAATCTAACA TTAGAGGGAA AATGTTCAGC CTGACAGATA CGGAGGACGA CGTCCAATTT 
GACACCTCGG ATTCAGACAC TAACTGTGAG GTGTTGCTGG CCGGAAAACA GTATCCATCG 
ATTCAGGAAT TTGCATCTGC CATTCCCAAC CAACTTCTGC TTGGATCTCT ACTTGAACAC 
TTGTGCTTTG TTTATGAGAG TGATCCCACA CGGTCACGCA TGCTGTTTAA AGTGATAGGC 
CAGCGGTTAG CGGCTATGAA CCTCCTGTCC CCACTGGCGA TCAGTGATGA GTTCAGCACG 
GTCCGATTAC AGCATAACAG GGCATTCACA GAGCTCCTGC ACGCCACAAG CTCATCCCTT 
TTCCCCCAGG AACATAAGTA CTTGAGTACA GAAGGCCAAA GCAAAAGAAC CAAAGATGGT 
CTTTTCCAAG CACAGACATC CCGGTACCTT AGTGAATTTG AAGAAATAGC AACACTGGGT 
AAAGGATCCT ATGGCAAAGT GTTTAGGGTC ACAAACAAGC TTGATGGTCA GGAATATGCA 
GTGAAAAAAA TTCTAATCAA GAAAGTAACA CGAGATGACT GTATGAAGGT TCTTAGAGAG 
GTTAAAGTGC TCTCCAGCCT TCAGCATCCC AATATTGTTG GATATCACAC TGCATGGATG 
GAACACGTTC AGCCTGCTGT GACGGATTCC AACTCTCTTG TTTCGCAAAC ATTTCCAGCA 
TTAGATAGAC CTTCAGAACC AAATGGGTGC ACTCCAGAAC ACACCAACAG CAGCAATGGA 
TCTTTCATTG TTTTTGAACA TTCTGAACGG GCAGAAGTGA AAGAAAATCA GGTTGCACTA 
GAGAGCATCT CGGTAAACCC CTCAACTTCA AAAGTGGACA TGGCACAGTC TGTTTGCCCT 
AAAAATGTCC GCAAGCCACA GAATTTTGTT CCATGTGTGT TTCTGGGAAA ACCCCGCTCT 
ACGTTAAACA AATGCCCTGC TGCTAATTGG GACAGTTCAA CACTCTCAGA GGATGACCTC 
AGTCAGAACA GACTGGAATT GAACAAACAC TCTTACATTG ACATGGACAC ACCCGAATGG 
TCTGAAAAGC AGGAGAACAA GGTTCAGTTC CACTTAATGC TTTATATACA AATGCAGCTG 
TGTGAACGAT CTTTGAAGGA CTGGATACAA GAGAGAAATT CAAGGACCAC GGAGGGACTC 
TTGATGTCAG GAGATCCCTG TGATTCAGTT GACTGTGAAC AGGCATTAAA GATTTTAAAG 
AAAATACTTG AAGGAGTGGA GTACATTCAT TCCTGTGGTA TTATGCACAG AGACCTAAAG 
CCAAGAAATA TTTTCCTTCT TGGAAATGAG TGCCATGTGA AGATAGGGGA TTTTGGATTG 
GCTTGCCAAA ACATTATAAT GGACGAACAT GAGAAGTTGC CTTCCAGTTC CCAAGCAGGT 
GTAAACCCAG ATGCAACTCA CACCAGTGGA GTGGGAACAT TTGTGTACGC TGCCCCAGAA 
CAACTTGAGG GCTCTCGCTA TGATTCAAAG TCAGATATGT ACAGCATAGG AGTGATTGCC 
TTTGAGTTAT TCCAGCCATT TGGGACCGAG ATGGAGCGTG TTCACACACT TGGAGAACTA 
CGACAGGGCA AGATCCCTAA CACACTGTCC ACAAACTGGC CAATTTTGGC CAAGTATATT 
AAACTACTGA CCTCCTCTGA TCCCTCAATG CGACCAAGTG CACCTCAGTT ACTTCAGAGT 
GACCTCTTCA GCACCAATGA CATGGTGATC CACAGTTTAA AGCGCAAGAT TGATGAACAA 
GAAGAAGAAA TTGTACAGCT CAGGAGACGG ATTAGTGAGC TACAGATCTC TCAGAATTCA 
ATACACAGTC CAGAAAAGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335808.1
CDS	198..2201
Translation	MFHRKETLMVVESSSNVSSVVSNVNSRSLHLQRGLSTQERESNIRGKMFSLTDTEDDVQFDTSDSDTNCEVLLAGKQYPSIQEFASAIPNQLLLGSLLEHLCFVYESDPTRSRMLFKVIGQRLAAMNLLSPLAISDEFSTVRLQHNRAFTELLHATSSSLFPQEHKYLSTEGQSKRTKDGLFQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIKKVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVTDSNSLVSQTFPALDRPSEPNGCTPEHTNSSNGSFIVFEHSERAEVKENQVALESISVNPSTSKVDMAQSVCPKNVRKPQNFVPCVFLGKPRSTLNKCPAANWDSSTLSEDDLSQNRLELNKHSYIDMDTPEWSEKQENKVQFHLMLYIQMQLCERSLKDWIQERNSRTTEGLLMSGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLLGNECHVKIGDFGLACQNIIMDEHEKLPSSSQAGVNPDATHTSGVGTFVYAAPEQLEGSRYDSKSDMYSIGVIAFELFQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTSSDPSMRPSAPQLLQSDLFSTNDMVIHSLKRKIDEQEEEIVQLRRRISELQISQNSIHSPEKT

Target ORF information:

RefSeq Version	XM_021480133.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480133.1

ORF Insert Sequence:

ATGTTTCACA GAAAAGAGAC CCTCATGGTA GTCGAAAGTT CGAGTAACGT TAGCAGTGTA 
GTTTCCAATG TTAACAGCCG TAGCCTTCAT TTACAACGGG GCCTTTCCAC CCAAGAGAGA 
GAATCTAACA TTAGAGGGAA AATGTTCAGC CTGACAGATA CGGAGGACGA CGTCCAATTT 
GACACCTCGG ATTCAGACAC TAACTGTGAG GTGTTGCTGG CCGGAAAACA GTATCCATCG 
ATTCAGGAAT TTGCATCTGC CATTCCCAAC CAACTTCTGC TTGGATCTCT ACTTGAACAC 
TTGTGCTTTG TTTATGAGAG TGATCCCACA CGGTCACGCA TGCTGTTTAA AGTGATAGGC 
CAGCGGTTAG CGGCTATGAA CCTCCTGTCC CCACTGGCGA TCAGTGATGA GTTCAGCACG 
GTCCGATTAC AGCATAACAG GGCATTCACA GAGCTCCTGC ACGCCACAAG CTCATCCCTT 
TTCCCCCAGG AACATAAGTA CTTGAGTACA GAAGGCCAAA GCAAAAGAAC CAAAGATGGT 
CTTTTCCAAG CACAGACATC CCGGTACCTT AGTGAATTTG AAGAAATAGC AACACTGGGT 
AAAGGATCCT ATGGCAAAGT GTTTAGGGTC ACAAACAAGC TTGATGGTCA GGAATATGCA 
GTGAAAAAAA TTCTAATCAA GAAAGTAACA CGAGATGACT GTATGAAGGT TCTTAGAGAG 
GTTAAAGTGC TCTCCAGCCT TCAGCATCCC AATATTGTTG GATATCACAC TGCATGGATG 
GAACACGTTC AGCCTGCTGT GACGGATTCC AACTCTCTTG TTTCGCAAAC ATTTCCAGCA 
TTAGATAGAC CTTCAGAACC AAATGGGTGC ACTCCAGAAC ACACCAACAG CAGCAATGGA 
TCTTTCATTG TTTTTGAACA TTCTGAACGG GCAGAAGTGA AAGAAAATCA GGTTGCACTA 
GAGAGCATCT CGGTAAACCC CTCAACTTCA AAAGTGGACA TGGCACAGTC TGTTTGCCCT 
AAAAATGTCC GCAAGCCACA GAATTTTGTT CCATGTGTGT TTCTGGGAAA ACCCCGCTCT 
ACGTTAAACA AATGCCCTGC TGCTAATTGG GACAGTTCAA CACTCTCAGA GGATGACCTC 
AGTCAGAACA GACTGGAATT GAACAAACAC TCTTACATTG ACATGGACAC ACCCGAATGG 
TCTGAAAAGC AGGAGAACAA GGTTCAGTTC CACTTAATGC TTTATATACA AATGCAGCTG 
TGTGAACGAT CTTTGAAGGA CTGGATACAA GAGAGAAATT CAAGGACCAC GGAGGGACTC 
TTGATGTCAG GAGATCCCTG TGATTCAGTT GACTGTGAAC AGGCATTAAA GATTTTAAAG 
AAAATACTTG AAGGAGTGGA GTACATTCAT TCCTGTGGTA TTATGCACAG AGACCTAAAG 
CCAAGAAATA TTTTCCTTCT TGGAAATGAG TGCCATGTGA AGATAGGGGA TTTTGGATTG 
GCTTGCCAAA ACATTATAAT GGACGAACAT GAGAAGTTGC CTTCCAGTTC CCAAGCAGGT 
GTAAACCCAG ATGCAACTCA CACCAGTGGA GTGGGAACAT TTGTGTACGC TGCCCCAGAA 
CAACTTGAGG GCTCTCGCTA TGATTCAAAG TCAGATATGT ACAGCATAGG AGTGATTGCC 
TTTGAGTTAT TCCAGCCATT TGGGACCGAG ATGGAGCGTG TTCACACACT TGGAGAACTA 
CGACAGGGCA AGATCCCTAA CACACTGTCC ACAAACTGGC CAATTTTGGC CAAGTATATT 
AAACTACTGA CCTCCTCTGA TCCCTCAATG CGACCAAGTG CACCTCAGTT ACTTCAGAGT 
GACCTCTTCA GCACCAATGA CATGGTGATC CACAGTTTAA AGCGCAAGAT TGATGAACAA 
GAAGAAGAAA TTGTACAGCT CAGGAGACGG ATTAGTGAGC TACAGATCTC TCAGAATTCA 
ATACACAGTC CAGAAAAGAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak1 ( XM_021480134.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335809.1 Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X2, mRNA.

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CloneID	ODa17750
Clone ID Related Accession (Same CDS sequence)	XM_021480134.1
Accession Version	XM_021480134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAA AAGAAAATGA TATTATGGCT TTAATTGCCA AAGCTATTGC TGAAGCCTCG 
GATTCAGACA CTAACTGTGA GGTGTTGCTG GCCGGAAAAC AGTATCCATC GATTCAGGAA 
TTTGCATCTG CCATTCCCAA CCAACTTCTG CTTGGATCTC TACTTGAACA CTTGTGCTTT 
GTTTATGAGA GTGATCCCAC ACGGTCACGC ATGCTGTTTA AAGTGATAGG CCAGCGGTTA 
GCGGCTATGA ACCTCCTGTC CCCACTGGCG ATCAGTGATG AGTTCAGCAC GGTCCGATTA 
CAGCATAACA GGGCATTCAC AGAGCTCCTG CACGCCACAA GCTCATCCCT TTTCCCCCAG 
GAACATAAGT ACTTGAGTAC AGAAGGCCAA AGCAAAAGAA CCAAAGATGG TCTTTTCCAA 
GCACAGACAT CCCGGTACCT TAGTGAATTT GAAGAAATAG CAACACTGGG TAAAGGATCC 
TATGGCAAAG TGTTTAGGGT CACAAACAAG CTTGATGGTC AGGAATATGC AGTGAAAAAA 
ATTCTAATCA AGAAAGTAAC ACGAGATGAC TGTATGAAGG TTCTTAGAGA GGTTAAAGTG 
CTCTCCAGCC TTCAGCATCC CAATATTGTT GGATATCACA CTGCATGGAT GGAACACGTT 
CAGCCTGCTG TGACGGTAGA TTCCAACTCT CTTGTTTCGC AAACATTTCC AGCATTAGAT 
AGACCTTCAG AACCAAATGG GTGCACTCCA GAACACACCA ACAGCAGCAA TGGATCTTTC 
ATTGTTTTTG AACATTCTGA ACGGGCAGAA GTGAAAGAAA ATCAGGTTGC ACTAGAGAGC 
ATCTCGGTAA ACCCCTCAAC TTCAAAAGTG GACATGGCAC AGTCTGTTTG CCCTAAAAAT 
GTCCGCAAGC CACAGAATTT TGTTCCATGT GTGTTTCTGG GAAAACCCCG CTCTACGTTA 
AACAAATGCC CTGCTGCTAA TTGGGACAGT TCAACACTCT CAGAGGATGA CCTCAGTCAG 
AACAGACTGG AATTGAACAA ACACTCTTAC ATTGACATGG ACACACCCGA ATGGTCTGAA 
AAGCAGGAGA ACAAGGTTCA GTTCCACTTA ATGCTTTATA TACAAATGCA GCTGTGTGAA 
CGATCTTTGA AGGACTGGAT ACAAGAGAGA AATTCAAGGA CCACGGAGGG ACTCTTGATG 
TCAGGAGATC CCTGTGATTC AGTTGACTGT GAACAGGCAT TAAAGATTTT AAAGAAAATA 
CTTGAAGGAG TGGAGTACAT TCATTCCTGT GGTATTATGC ACAGAGACCT AAAGCCAAGA 
AATATTTTCC TTCTTGGAAA TGAGTGCCAT GTGAAGATAG GGGATTTTGG ATTGGCTTGC 
CAAAACATTA TAATGGACGA ACATGAGAAG TTGCCTTCCA GTTCCCAAGC AGGTGTAAAC 
CCAGATGCAA CTCACACCAG TGGAGTGGGA ACATTTGTGT ACGCTGCCCC AGAACAACTT 
GAGGGCTCTC GCTATGATTC AAAGTCAGAT ATGTACAGCA TAGGAGTGAT TGCCTTTGAG 
TTATTCCAGC CATTTGGGAC CGAGATGGAG CGTGTTCACA CACTTGGAGA ACTACGACAG 
GGCAAGATCC CTAACACACT GTCCACAAAC TGGCCAATTT TGGCCAAGTA TATTAAACTA 
CTGACCTCCT CTGATCCCTC AATGCGACCA AGTGCACCTC AGTTACTTCA GAGTGACCTC 
TTCAGCACCA ATGACATGGT GATCCACAGT TTAAAGCGCA AGATTGATGA ACAAGAAGAA 
GAAATTGTAC AGCTCAGGAG ACGGATTAGT GAGCTACAGA TCTCTCAGAA TTCAATACAC 
AGTCCAGAAA AGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335809.1
CDS	351..2228
Translation	MDSKENDIMALIAKAIAEASDSDTNCEVLLAGKQYPSIQEFASAIPNQLLLGSLLEHLCFVYESDPTRSRMLFKVIGQRLAAMNLLSPLAISDEFSTVRLQHNRAFTELLHATSSSLFPQEHKYLSTEGQSKRTKDGLFQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIKKVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVTVDSNSLVSQTFPALDRPSEPNGCTPEHTNSSNGSFIVFEHSERAEVKENQVALESISVNPSTSKVDMAQSVCPKNVRKPQNFVPCVFLGKPRSTLNKCPAANWDSSTLSEDDLSQNRLELNKHSYIDMDTPEWSEKQENKVQFHLMLYIQMQLCERSLKDWIQERNSRTTEGLLMSGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLLGNECHVKIGDFGLACQNIIMDEHEKLPSSSQAGVNPDATHTSGVGTFVYAAPEQLEGSRYDSKSDMYSIGVIAFELFQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTSSDPSMRPSAPQLLQSDLFSTNDMVIHSLKRKIDEQEEEIVQLRRRISELQISQNSIHSPEKT

Target ORF information:

RefSeq Version	XM_021480134.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 1 (eif2ak1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480134.1

ORF Insert Sequence:

ATGGATTCAA AAGAAAATGA TATTATGGCT TTAATTGCCA AAGCTATTGC TGAAGCCTCG 
GATTCAGACA CTAACTGTGA GGTGTTGCTG GCCGGAAAAC AGTATCCATC GATTCAGGAA 
TTTGCATCTG CCATTCCCAA CCAACTTCTG CTTGGATCTC TACTTGAACA CTTGTGCTTT 
GTTTATGAGA GTGATCCCAC ACGGTCACGC ATGCTGTTTA AAGTGATAGG CCAGCGGTTA 
GCGGCTATGA ACCTCCTGTC CCCACTGGCG ATCAGTGATG AGTTCAGCAC GGTCCGATTA 
CAGCATAACA GGGCATTCAC AGAGCTCCTG CACGCCACAA GCTCATCCCT TTTCCCCCAG 
GAACATAAGT ACTTGAGTAC AGAAGGCCAA AGCAAAAGAA CCAAAGATGG TCTTTTCCAA 
GCACAGACAT CCCGGTACCT TAGTGAATTT GAAGAAATAG CAACACTGGG TAAAGGATCC 
TATGGCAAAG TGTTTAGGGT CACAAACAAG CTTGATGGTC AGGAATATGC AGTGAAAAAA 
ATTCTAATCA AGAAAGTAAC ACGAGATGAC TGTATGAAGG TTCTTAGAGA GGTTAAAGTG 
CTCTCCAGCC TTCAGCATCC CAATATTGTT GGATATCACA CTGCATGGAT GGAACACGTT 
CAGCCTGCTG TGACGGTAGA TTCCAACTCT CTTGTTTCGC AAACATTTCC AGCATTAGAT 
AGACCTTCAG AACCAAATGG GTGCACTCCA GAACACACCA ACAGCAGCAA TGGATCTTTC 
ATTGTTTTTG AACATTCTGA ACGGGCAGAA GTGAAAGAAA ATCAGGTTGC ACTAGAGAGC 
ATCTCGGTAA ACCCCTCAAC TTCAAAAGTG GACATGGCAC AGTCTGTTTG CCCTAAAAAT 
GTCCGCAAGC CACAGAATTT TGTTCCATGT GTGTTTCTGG GAAAACCCCG CTCTACGTTA 
AACAAATGCC CTGCTGCTAA TTGGGACAGT TCAACACTCT CAGAGGATGA CCTCAGTCAG 
AACAGACTGG AATTGAACAA ACACTCTTAC ATTGACATGG ACACACCCGA ATGGTCTGAA 
AAGCAGGAGA ACAAGGTTCA GTTCCACTTA ATGCTTTATA TACAAATGCA GCTGTGTGAA 
CGATCTTTGA AGGACTGGAT ACAAGAGAGA AATTCAAGGA CCACGGAGGG ACTCTTGATG 
TCAGGAGATC CCTGTGATTC AGTTGACTGT GAACAGGCAT TAAAGATTTT AAAGAAAATA 
CTTGAAGGAG TGGAGTACAT TCATTCCTGT GGTATTATGC ACAGAGACCT AAAGCCAAGA 
AATATTTTCC TTCTTGGAAA TGAGTGCCAT GTGAAGATAG GGGATTTTGG ATTGGCTTGC 
CAAAACATTA TAATGGACGA ACATGAGAAG TTGCCTTCCA GTTCCCAAGC AGGTGTAAAC 
CCAGATGCAA CTCACACCAG TGGAGTGGGA ACATTTGTGT ACGCTGCCCC AGAACAACTT 
GAGGGCTCTC GCTATGATTC AAAGTCAGAT ATGTACAGCA TAGGAGTGAT TGCCTTTGAG 
TTATTCCAGC CATTTGGGAC CGAGATGGAG CGTGTTCACA CACTTGGAGA ACTACGACAG 
GGCAAGATCC CTAACACACT GTCCACAAAC TGGCCAATTT TGGCCAAGTA TATTAAACTA 
CTGACCTCCT CTGATCCCTC AATGCGACCA AGTGCACCTC AGTTACTTCA GAGTGACCTC 
TTCAGCACCA ATGACATGGT GATCCACAGT TTAAAGCGCA AGATTGATGA ACAAGAAGAA 
GAAATTGTAC AGCTCAGGAG ACGGATTAGT GAGCTACAGA TCTCTCAGAA TTCAATACAC 
AGTCCAGAAA AGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos.
BMC developmental biology18(1)5(2018 03)
Tsai TC,Shih CC,Chien HP,Yang AH,Lu JK,Lu JH

A truncated Danio rerio PKZ isoform functionally interacts with eIF2α and inhibits protein synthesis.
Gene527(1)292-300(2013 Sep)
Liu ZY,Jia KT,Li C,Weng SP,Guo CJ,He JG

A shifted repertoire of endocannabinoid genes in the zebrafish (Danio rerio).
Molecular genetics and genomics : MGG277(5)555-70(2007 May)
McPartland JM,Glass M,Matias I,Norris RW,Kilpatrick CW

A PKR-like eukaryotic initiation factor 2alpha kinase from zebrafish contains Z-DNA binding domains instead of dsRNA binding domains.
Proceedings of the National Academy of Sciences of the United States of America102(5)1602-7(2005 Feb)
Rothenburg S,Deigendesch N,Dittmar K,Koch-Nolte F,Haag F,Lowenhaupt K,Rich A

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eif2ak1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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