EIF2AK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF2AK1
Entrez Gene ID	27102
Full Name	eukaryotic translation initiation factor 2 alpha kinase 1
Synonyms	HCR,HRI
General protein information	Preferred Names eukaryotic translation initiation factor 2 alpha kinase 1 Names eukaryotic translation initiation factor 2-alpha kinase 1 heme regulated initiation factor 2 alpha kinase heme sensitive initiation factor 2a kinase heme-controlled repressor heme-regulated eukaryotic initiation factor eIF-2-alpha kinase heme-regulated inhibitor heme-regulated repressor hemin-sensitive initiation factor 2-alpha kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p22.1
Summary	The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008].
Disorder MIM:	613635

mRNA and Protein(s)

mRNA	Protein	Name
XM_024446718.1	XP_024302486.1	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
NM_001134335.1	NP_001127807.1	eukaryotic translation initiation factor 2-alpha kinase 1 isoform b
NM_014413.3	NP_055228.2	eukaryotic translation initiation factor 2-alpha kinase 1 isoform a
NM_014413.4	NP_055228.2	eukaryotic translation initiation factor 2-alpha kinase 1 isoform a

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	hri2	NP_593146.1
Macaca mulatta (Rhesus monkey)	EIF2AK1	XP_001089983.2
Rattus norvegicus (Norway rat)	Eif2ak1	NP_037355.1
Danio rerio (zebrafish)	eif2ak1	NP_001071035.1
Canis lupus familiaris (dog)	EIF2AK1	NP_001155185.1
Gallus gallus (chicken)	EIF2AK1	NP_989979.1
Pan troglodytes (chimpanzee)	EIF2AK1	XP_518962.3
Homo sapiens (human)	EIF2AK1	NP_001127807.1
Mus musculus (house mouse)	Eif2ak1	NP_038585.2
Xenopus tropicalis (tropical clawed frog)	eif2ak1	NP_001039038.1
Schizosaccharomyces pombe (fission yeast)	hri1	NP_594738.1
Bos taurus (cattle)	EIF2AK1	NP_001033142.1

Gene Ontology
Bibliography

Related articles in PubMed

The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.
Abdel-Nour M, Carneiro LAM, Downey J, Tsalikis J, Outlioua A, Prescott D, Da Costa LS, Hovingh ES, Farahvash A, Gaudet RG, Molinaro R, van Dalen R, Lau CCY, Azimi FC, Escalante NK, Trotman-Grant A, Lee JE, Gray-Owen SD, Divangahi M, Chen JJ, Philpott DJ, Arnoult D, Girardin SE
Science (New York, N.Y.)365(6448)(2019 07)

Activation of HRI is mediated by Hsp90 during stress through modulation of the HRI-Hsp90 complex.
Berwal SK, Bhatia V, Bendre A, Suresh CG, Chatterjee S, Pal JK
International journal of biological macromolecules118(Pt B)1604-1613(2018 Oct)

Domain-focused CRISPR screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells.
Grevet JD, Lan X, Hamagami N, Edwards CR, Sankaranarayanan L, Ji X, Bhardwaj SK, Face CJ, Posocco DF, Abdulmalik O, Keller CA, Giardine B, Sidoli S, Garcia BA, Chou ST, Liebhaber SA, Hardison RC, Shi J, Blobel GA
Science (New York, N.Y.)361(6399)285-290(2018 07)

An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Öhman T, Varjosalo M
Nature communications9(1)1188(2018 03)

Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1.
Vincent HA, Ziehr B, Moorman NJ
Journal of virology91(5)(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EIF2AK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2AK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu105878	XM_024446718.1 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu15053	NM_001134335.1 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu14846	NM_014413.4 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu14846	NM_014413.3	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF2AK1 ( XM_024446718.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302486.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant X1, mRNA.

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CloneID	OHu105878
Clone ID Related Accession (Same CDS sequence)	XM_024446718.1
Accession Version	XM_024446718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCC TACGGGAAGT GAAGGTGCTG GCAGGTCTTC AGCACCCCAA TATTGTTGGC 
TATCACACCG CGTGGATAGA ACATGTTCAT GTGATTCAGC CACGAGCAGA CAGAGCTGCC 
ATTGAGTTGC CATCTCTGGA AGTGCTCTCC GACCAGGAAG AGGACAGAGA GCAATGTGGT 
GTTAAAAATG ATGAAAGTAG CAGCTCATCC ATTATCTTTG CTGAGCCCAC CCCAGAAAAA 
GAAAAACGCT TTGGAGAATC TGACACTGAA AATCAGAATA ACAAGTCGGT GAAGTACACC 
ACCAATTTAG TCATAAGAGA ATCTGGTGAA CTTGAGTCGA CCCTGGAGCT CCAGGAAAAT 
GGCTTGGCTG GTTTGTCTGC CAGTTCAATT GTGGAACAGC AGCTGCCACT CAGGCGTAAT 
TCCCACCTAG AGGAGAGTTT CACATCCACC GAAGAATCTT CCGAAGAAAA TGTCAACTTT 
TTGGGTCAGA CAGAGGCACA GTACCACCTG ATGCTGCACA TCCAGATGCA GCTGTGTGAG 
CTCTCGCTGT GGGATTGGAT AGTCGAGAGA AACAAGCGGG GCCGGGAGTA TGTGGACGAG 
TCTGCCTGTC CTTATGTTAT GGCCAATGTT GCAACAAAAA TTTTTCAAGA ATTGGTAGAA 
GGTGTGTTTT ACATACATAA CATGGGAATT GTGCACCGAG ATCTGAAGCC AAGAAATATT 
TTTCTTCATG GCCCTGATCA GCAAGTAAAA ATAGGAGACT TTGGTCTGGC CTGCACAGAC 
ATCCTACAGA AGAACACAGA CTGGACCAAC AGAAACGGGA AGAGAACACC AACACATACG 
TCCAGAGTGG GTACTTGTCT GTACGCTTCA CCCGAACAGT TGGAAGGATC TGAGTATGAT 
GCCAAGTCAG ATATGTACAG CTTGGGTGTG GTCCTGCTAG AGCTCTTTCA GCCGTTTGGA 
ACAGAAATGG AGCGAGCAGA AGTTCTAACA GGTTTAAGAA CTGGTCAGTT GCCGGAATCC 
CTCCGTAAAA GGTGTCCAGT GCAAGCCAAG TATATCCAGC ACTTAACGAG AAGGAACTCA 
TCGCAGAGAC CATCTGCCAT TCAGCTGCTG CAGAGTGAAC TTTTCCAAAA TTCTGGAAAT 
GTTAACCTCA CCCTACAGAT GAAGATAATA GAGCAAGAAA AAGAAATTGC AGAACTAAAG 
AAGCAGCTAA ACCTCCTTTC TCAAGACAAA GGGGTGAGGG ATGACGGAAA GGATGGGGGC 
GTGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302486.1
CDS	889..2157
Translation	MKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSIIFAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

Target ORF information:

RefSeq Version	XM_024446718.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446718.1

ORF Insert Sequence:

ATGAAGGTCC TACGGGAAGT GAAGGTGCTG GCAGGTCTTC AGCACCCCAA TATTGTTGGC 
TATCACACCG CGTGGATAGA ACATGTTCAT GTGATTCAGC CACGAGCAGA CAGAGCTGCC 
ATTGAGTTGC CATCTCTGGA AGTGCTCTCC GACCAGGAAG AGGACAGAGA GCAATGTGGT 
GTTAAAAATG ATGAAAGTAG CAGCTCATCC ATTATCTTTG CTGAGCCCAC CCCAGAAAAA 
GAAAAACGCT TTGGAGAATC TGACACTGAA AATCAGAATA ACAAGTCGGT GAAGTACACC 
ACCAATTTAG TCATAAGAGA ATCTGGTGAA CTTGAGTCGA CCCTGGAGCT CCAGGAAAAT 
GGCTTGGCTG GTTTGTCTGC CAGTTCAATT GTGGAACAGC AGCTGCCACT CAGGCGTAAT 
TCCCACCTAG AGGAGAGTTT CACATCCACC GAAGAATCTT CCGAAGAAAA TGTCAACTTT 
TTGGGTCAGA CAGAGGCACA GTACCACCTG ATGCTGCACA TCCAGATGCA GCTGTGTGAG 
CTCTCGCTGT GGGATTGGAT AGTCGAGAGA AACAAGCGGG GCCGGGAGTA TGTGGACGAG 
TCTGCCTGTC CTTATGTTAT GGCCAATGTT GCAACAAAAA TTTTTCAAGA ATTGGTAGAA 
GGTGTGTTTT ACATACATAA CATGGGAATT GTGCACCGAG ATCTGAAGCC AAGAAATATT 
TTTCTTCATG GCCCTGATCA GCAAGTAAAA ATAGGAGACT TTGGTCTGGC CTGCACAGAC 
ATCCTACAGA AGAACACAGA CTGGACCAAC AGAAACGGGA AGAGAACACC AACACATACG 
TCCAGAGTGG GTACTTGTCT GTACGCTTCA CCCGAACAGT TGGAAGGATC TGAGTATGAT 
GCCAAGTCAG ATATGTACAG CTTGGGTGTG GTCCTGCTAG AGCTCTTTCA GCCGTTTGGA 
ACAGAAATGG AGCGAGCAGA AGTTCTAACA GGTTTAAGAA CTGGTCAGTT GCCGGAATCC 
CTCCGTAAAA GGTGTCCAGT GCAAGCCAAG TATATCCAGC ACTTAACGAG AAGGAACTCA 
TCGCAGAGAC CATCTGCCAT TCAGCTGCTG CAGAGTGAAC TTTTCCAAAA TTCTGGAAAT 
GTTAACCTCA CCCTACAGAT GAAGATAATA GAGCAAGAAA AAGAAATTGC AGAACTAAAG 
AAGCAGCTAA ACCTCCTTTC TCAAGACAAA GGGGTGAGGG ATGACGGAAA GGATGGGGGC 
GTGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK1 ( NM_001134335.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127807.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15053
Clone ID Related Accession (Same CDS sequence)	NM_001134335.1
Accession Version	NM_001134335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC341983.1, AL136563.1 and AC005995.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is 1 aa shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.46016.1, SRR1803613.204628.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGGG GCAACTCCGG GGTCCGCAAG CGCGAAGAGG AGGGCGACGG GGCTGGGGCT 
GTGGCTGCGC CGCCGGCCAT CGACTTTCCC GCCGAGGGCC CGGACCCCGA ATATGACGAA 
TCTGATGTTC CAGCAGAAAT CCAGGTGTTA AAAGAACCCC TACAACAGCC AACCTTCCCT 
TTTGCAGTTG CAAACCAACT CTTGCTGGTT TCTTTGCTGG AGCACTTGAG CCACGTGCAT 
GAACCAAACC CACTTCGTTC AAGACAGGTG TTTAAGCTAC TTTGCCAGAC GTTTATCAAA 
ATGGGGCTGC TGTCTTCTTT CACTTGTAGT GACGAGTTTA GCTCATTGAG ACTACATCAC 
AACAGAGCTA TTACTCACTT AATGAGGTCT GCTAAAGAGA GAGTTCGTCA GGATCCTTGT 
GAGGATATTT CTCGTATCCA GAAAATCAGA TCAAGGGAAG TAGCCTTGGA AGCACAAACT 
TCACGTTACT TAAATGAATT TGAAGAACTT GCCATCTTAG GAAAAGGTGG ATACGGAAGA 
GTATACAAGG TCAGGAATAA ATTAGATGGT CAGTATTATG CAATAAAAAA AATCCTGATT 
AAGGGTGCAA CTAAAACAGT TTGCATGAAG GTCCTACGGG AAGTGAAGGT GCTGGCAGGT 
CTTCAGCACC CCAATATTGT TGGCTATCAC ACCGCGTGGA TAGAACATGT TCATGTGATT 
CAGCCACGAG ACAGAGCTGC CATTGAGTTG CCATCTCTGG AAGTGCTCTC CGACCAGGAA 
GAGGACAGAG AGCAATGTGG TGTTAAAAAT GATGAAAGTA GCAGCTCATC CATTATCTTT 
GCTGAGCCCA CCCCAGAAAA AGAAAAACGC TTTGGAGAAT CTGACACTGA AAATCAGAAT 
AACAAGTCGG TGAAGTACAC CACCAATTTA GTCATAAGAG AATCTGGTGA ACTTGAGTCG 
ACCCTGGAGC TCCAGGAAAA TGGCTTGGCT GGTTTGTCTG CCAGTTCAAT TGTGGAACAG 
CAGCTGCCAC TCAGGCGTAA TTCCCACCTA GAGGAGAGTT TCACATCCAC CGAAGAATCT 
TCCGAAGAAA ATGTCAACTT TTTGGGTCAG ACAGAGGCAC AGTACCACCT GATGCTGCAC 
ATCCAGATGC AGCTGTGTGA GCTCTCGCTG TGGGATTGGA TAGTCGAGAG AAACAAGCGG 
GGCCGGGAGT ATGTGGACGA GTCTGCCTGT CCTTATGTTA TGGCCAATGT TGCAACAAAA 
ATTTTTCAAG AATTGGTAGA AGGTGTGTTT TACATACATA ACATGGGAAT TGTGCACCGA 
GATCTGAAGC CAAGAAATAT TTTTCTTCAT GGCCCTGATC AGCAAGTAAA AATAGGAGAC 
TTTGGTCTGG CCTGCACAGA CATCCTACAG AAGAACACAG ACTGGACCAA CAGAAACGGG 
AAGAGAACAC CAACACATAC GTCCAGAGTG GGTACTTGTC TGTACGCTTC ACCCGAACAG 
TTGGAAGGAT CTGAGTATGA TGCCAAGTCA GATATGTACA GCTTGGGTGT GGTCCTGCTA 
GAGCTCTTTC AGCCGTTTGG AACAGAAATG GAGCGAGCAG AAGTTCTAAC AGGTTTAAGA 
ACTGGTCAGT TGCCGGAATC CCTCCGTAAA AGGTGTCCAG TGCAAGCCAA GTATATCCAG 
CACTTAACGA GAAGGAACTC ATCGCAGAGA CCATCTGCCA TTCAGCTGCT GCAGAGTGAA 
CTTTTCCAAA ATTCTGGAAA TGTTAACCTC ACCCTACAGA TGAAGATAAT AGAGCAAGAA 
AAAGAAATTG CAGAACTAAA GAAGCAGCTA AACCTCCTTT CTCAAGACAA AGGGGTGAGG 
GATGACGGAA AGGATGGGGG CGTGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127807.1
CDS	147..2036
Translation	MQGGNSGVRKREEEGDGAGAVAAPPAIDFPAEGPDPEYDESDVPAEIQVLKEPLQQPTFPFAVANQLLLVSLLEHLSHVHEPNPLRSRQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCEDISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRDRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSIIFAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

Target ORF information:

RefSeq Version	NM_001134335.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134335.1

ORF Insert Sequence:

ATGCAGGGGG GCAACTCCGG GGTCCGCAAG CGCGAAGAGG AGGGCGACGG GGCTGGGGCT 
GTGGCTGCGC CGCCGGCCAT CGACTTTCCC GCCGAGGGCC CGGACCCCGA ATATGACGAA 
TCTGATGTTC CAGCAGAAAT CCAGGTGTTA AAAGAACCCC TACAACAGCC AACCTTCCCT 
TTTGCAGTTG CAAACCAACT CTTGCTGGTT TCTTTGCTGG AGCACTTGAG CCACGTGCAT 
GAACCAAACC CACTTCGTTC AAGACAGGTG TTTAAGCTAC TTTGCCAGAC GTTTATCAAA 
ATGGGGCTGC TGTCTTCTTT CACTTGTAGT GACGAGTTTA GCTCATTGAG ACTACATCAC 
AACAGAGCTA TTACTCACTT AATGAGGTCT GCTAAAGAGA GAGTTCGTCA GGATCCTTGT 
GAGGATATTT CTCGTATCCA GAAAATCAGA TCAAGGGAAG TAGCCTTGGA AGCACAAACT 
TCACGTTACT TAAATGAATT TGAAGAACTT GCCATCTTAG GAAAAGGTGG ATACGGAAGA 
GTATACAAGG TCAGGAATAA ATTAGATGGT CAGTATTATG CAATAAAAAA AATCCTGATT 
AAGGGTGCAA CTAAAACAGT TTGCATGAAG GTCCTACGGG AAGTGAAGGT GCTGGCAGGT 
CTTCAGCACC CCAATATTGT TGGCTATCAC ACCGCGTGGA TAGAACATGT TCATGTGATT 
CAGCCACGAG ACAGAGCTGC CATTGAGTTG CCATCTCTGG AAGTGCTCTC CGACCAGGAA 
GAGGACAGAG AGCAATGTGG TGTTAAAAAT GATGAAAGTA GCAGCTCATC CATTATCTTT 
GCTGAGCCCA CCCCAGAAAA AGAAAAACGC TTTGGAGAAT CTGACACTGA AAATCAGAAT 
AACAAGTCGG TGAAGTACAC CACCAATTTA GTCATAAGAG AATCTGGTGA ACTTGAGTCG 
ACCCTGGAGC TCCAGGAAAA TGGCTTGGCT GGTTTGTCTG CCAGTTCAAT TGTGGAACAG 
CAGCTGCCAC TCAGGCGTAA TTCCCACCTA GAGGAGAGTT TCACATCCAC CGAAGAATCT 
TCCGAAGAAA ATGTCAACTT TTTGGGTCAG ACAGAGGCAC AGTACCACCT GATGCTGCAC 
ATCCAGATGC AGCTGTGTGA GCTCTCGCTG TGGGATTGGA TAGTCGAGAG AAACAAGCGG 
GGCCGGGAGT ATGTGGACGA GTCTGCCTGT CCTTATGTTA TGGCCAATGT TGCAACAAAA 
ATTTTTCAAG AATTGGTAGA AGGTGTGTTT TACATACATA ACATGGGAAT TGTGCACCGA 
GATCTGAAGC CAAGAAATAT TTTTCTTCAT GGCCCTGATC AGCAAGTAAA AATAGGAGAC 
TTTGGTCTGG CCTGCACAGA CATCCTACAG AAGAACACAG ACTGGACCAA CAGAAACGGG 
AAGAGAACAC CAACACATAC GTCCAGAGTG GGTACTTGTC TGTACGCTTC ACCCGAACAG 
TTGGAAGGAT CTGAGTATGA TGCCAAGTCA GATATGTACA GCTTGGGTGT GGTCCTGCTA 
GAGCTCTTTC AGCCGTTTGG AACAGAAATG GAGCGAGCAG AAGTTCTAAC AGGTTTAAGA 
ACTGGTCAGT TGCCGGAATC CCTCCGTAAA AGGTGTCCAG TGCAAGCCAA GTATATCCAG 
CACTTAACGA GAAGGAACTC ATCGCAGAGA CCATCTGCCA TTCAGCTGCT GCAGAGTGAA 
CTTTTCCAAA ATTCTGGAAA TGTTAACCTC ACCCTACAGA TGAAGATAAT AGAGCAAGAA 
AAAGAAATTG CAGAACTAAA GAAGCAGCTA AACCTCCTTT CTCAAGACAA AGGGGTGAGG 
GATGACGGAA AGGATGGGGG CGTGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK1 ( NM_014413.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055228.2 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14846
Clone ID Related Accession (Same CDS sequence)	NM_014413.3 , NM_014413.4
Accession Version	NM_014413.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC341983.1, BC006524.2 and AC005995.3. On Aug 23, 2008 this sequence version replaced NM_014413.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.269101.1, SRR1660805.60123.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGGG GCAACTCCGG GGTCCGCAAG CGCGAAGAGG AGGGCGACGG GGCTGGGGCT 
GTGGCTGCGC CGCCGGCCAT CGACTTTCCC GCCGAGGGCC CGGACCCCGA ATATGACGAA 
TCTGATGTTC CAGCAGAAAT CCAGGTGTTA AAAGAACCCC TACAACAGCC AACCTTCCCT 
TTTGCAGTTG CAAACCAACT CTTGCTGGTT TCTTTGCTGG AGCACTTGAG CCACGTGCAT 
GAACCAAACC CACTTCGTTC AAGACAGGTG TTTAAGCTAC TTTGCCAGAC GTTTATCAAA 
ATGGGGCTGC TGTCTTCTTT CACTTGTAGT GACGAGTTTA GCTCATTGAG ACTACATCAC 
AACAGAGCTA TTACTCACTT AATGAGGTCT GCTAAAGAGA GAGTTCGTCA GGATCCTTGT 
GAGGATATTT CTCGTATCCA GAAAATCAGA TCAAGGGAAG TAGCCTTGGA AGCACAAACT 
TCACGTTACT TAAATGAATT TGAAGAACTT GCCATCTTAG GAAAAGGTGG ATACGGAAGA 
GTATACAAGG TCAGGAATAA ATTAGATGGT CAGTATTATG CAATAAAAAA AATCCTGATT 
AAGGGTGCAA CTAAAACAGT TTGCATGAAG GTCCTACGGG AAGTGAAGGT GCTGGCAGGT 
CTTCAGCACC CCAATATTGT TGGCTATCAC ACCGCGTGGA TAGAACATGT TCATGTGATT 
CAGCCACGAG CAGACAGAGC TGCCATTGAG TTGCCATCTC TGGAAGTGCT CTCCGACCAG 
GAAGAGGACA GAGAGCAATG TGGTGTTAAA AATGATGAAA GTAGCAGCTC ATCCATTATC 
TTTGCTGAGC CCACCCCAGA AAAAGAAAAA CGCTTTGGAG AATCTGACAC TGAAAATCAG 
AATAACAAGT CGGTGAAGTA CACCACCAAT TTAGTCATAA GAGAATCTGG TGAACTTGAG 
TCGACCCTGG AGCTCCAGGA AAATGGCTTG GCTGGTTTGT CTGCCAGTTC AATTGTGGAA 
CAGCAGCTGC CACTCAGGCG TAATTCCCAC CTAGAGGAGA GTTTCACATC CACCGAAGAA 
TCTTCCGAAG AAAATGTCAA CTTTTTGGGT CAGACAGAGG CACAGTACCA CCTGATGCTG 
CACATCCAGA TGCAGCTGTG TGAGCTCTCG CTGTGGGATT GGATAGTCGA GAGAAACAAG 
CGGGGCCGGG AGTATGTGGA CGAGTCTGCC TGTCCTTATG TTATGGCCAA TGTTGCAACA 
AAAATTTTTC AAGAATTGGT AGAAGGTGTG TTTTACATAC ATAACATGGG AATTGTGCAC 
CGAGATCTGA AGCCAAGAAA TATTTTTCTT CATGGCCCTG ATCAGCAAGT AAAAATAGGA 
GACTTTGGTC TGGCCTGCAC AGACATCCTA CAGAAGAACA CAGACTGGAC CAACAGAAAC 
GGGAAGAGAA CACCAACACA TACGTCCAGA GTGGGTACTT GTCTGTACGC TTCACCCGAA 
CAGTTGGAAG GATCTGAGTA TGATGCCAAG TCAGATATGT ACAGCTTGGG TGTGGTCCTG 
CTAGAGCTCT TTCAGCCGTT TGGAACAGAA ATGGAGCGAG CAGAAGTTCT AACAGGTTTA 
AGAACTGGTC AGTTGCCGGA ATCCCTCCGT AAAAGGTGTC CAGTGCAAGC CAAGTATATC 
CAGCACTTAA CGAGAAGGAA CTCATCGCAG AGACCATCTG CCATTCAGCT GCTGCAGAGT 
GAACTTTTCC AAAATTCTGG AAATGTTAAC CTCACCCTAC AGATGAAGAT AATAGAGCAA 
GAAAAAGAAA TTGCAGAACT AAAGAAGCAG CTAAACCTCC TTTCTCAAGA CAAAGGGGTG 
AGGGATGACG GAAAGGATGG GGGCGTGGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055228.2
CDS	147..2039
Translation	MQGGNSGVRKREEEGDGAGAVAAPPAIDFPAEGPDPEYDESDVPAEIQVLKEPLQQPTFPFAVANQLLLVSLLEHLSHVHEPNPLRSRQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCEDISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSIIFAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

Target ORF information:

RefSeq Version	NM_014413.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014413.3

ORF Insert Sequence:

ATGCAGGGGG GCAACTCCGG GGTCCGCAAG CGCGAAGAGG AGGGCGACGG GGCTGGGGCT 
GTGGCTGCGC CGCCGGCCAT CGACTTTCCC GCCGAGGGCC CGGACCCCGA ATATGACGAA 
TCTGATGTTC CAGCAGAAAT CCAGGTGTTA AAAGAACCCC TACAACAGCC AACCTTCCCT 
TTTGCAGTTG CAAACCAACT CTTGCTGGTT TCTTTGCTGG AGCACTTGAG CCACGTGCAT 
GAACCAAACC CACTTCGTTC AAGACAGGTG TTTAAGCTAC TTTGCCAGAC GTTTATCAAA 
ATGGGGCTGC TGTCTTCTTT CACTTGTAGT GACGAGTTTA GCTCATTGAG ACTACATCAC 
AACAGAGCTA TTACTCACTT AATGAGGTCT GCTAAAGAGA GAGTTCGTCA GGATCCTTGT 
GAGGATATTT CTCGTATCCA GAAAATCAGA TCAAGGGAAG TAGCCTTGGA AGCACAAACT 
TCACGTTACT TAAATGAATT TGAAGAACTT GCCATCTTAG GAAAAGGTGG ATACGGAAGA 
GTATACAAGG TCAGGAATAA ATTAGATGGT CAGTATTATG CAATAAAAAA AATCCTGATT 
AAGGGTGCAA CTAAAACAGT TTGCATGAAG GTCCTACGGG AAGTGAAGGT GCTGGCAGGT 
CTTCAGCACC CCAATATTGT TGGCTATCAC ACCGCGTGGA TAGAACATGT TCATGTGATT 
CAGCCACGAG CAGACAGAGC TGCCATTGAG TTGCCATCTC TGGAAGTGCT CTCCGACCAG 
GAAGAGGACA GAGAGCAATG TGGTGTTAAA AATGATGAAA GTAGCAGCTC ATCCATTATC 
TTTGCTGAGC CCACCCCAGA AAAAGAAAAA CGCTTTGGAG AATCTGACAC TGAAAATCAG 
AATAACAAGT CGGTGAAGTA CACCACCAAT TTAGTCATAA GAGAATCTGG TGAACTTGAG 
TCGACCCTGG AGCTCCAGGA AAATGGCTTG GCTGGTTTGT CTGCCAGTTC AATTGTGGAA 
CAGCAGCTGC CACTCAGGCG TAATTCCCAC CTAGAGGAGA GTTTCACATC CACCGAAGAA 
TCTTCCGAAG AAAATGTCAA CTTTTTGGGT CAGACAGAGG CACAGTACCA CCTGATGCTG 
CACATCCAGA TGCAGCTGTG TGAGCTCTCG CTGTGGGATT GGATAGTCGA GAGAAACAAG 
CGGGGCCGGG AGTATGTGGA CGAGTCTGCC TGTCCTTATG TTATGGCCAA TGTTGCAACA 
AAAATTTTTC AAGAATTGGT AGAAGGTGTG TTTTACATAC ATAACATGGG AATTGTGCAC 
CGAGATCTGA AGCCAAGAAA TATTTTTCTT CATGGCCCTG ATCAGCAAGT AAAAATAGGA 
GACTTTGGTC TGGCCTGCAC AGACATCCTA CAGAAGAACA CAGACTGGAC CAACAGAAAC 
GGGAAGAGAA CACCAACACA TACGTCCAGA GTGGGTACTT GTCTGTACGC TTCACCCGAA 
CAGTTGGAAG GATCTGAGTA TGATGCCAAG TCAGATATGT ACAGCTTGGG TGTGGTCCTG 
CTAGAGCTCT TTCAGCCGTT TGGAACAGAA ATGGAGCGAG CAGAAGTTCT AACAGGTTTA 
AGAACTGGTC AGTTGCCGGA ATCCCTCCGT AAAAGGTGTC CAGTGCAAGC CAAGTATATC 
CAGCACTTAA CGAGAAGGAA CTCATCGCAG AGACCATCTG CCATTCAGCT GCTGCAGAGT 
GAACTTTTCC AAAATTCTGG AAATGTTAAC CTCACCCTAC AGATGAAGAT AATAGAGCAA 
GAAAAAGAAA TTGCAGAACT AAAGAAGCAG CTAAACCTCC TTTCTCAAGA CAAAGGGGTG 
AGGGATGACG GAAAGGATGG GGGCGTGGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK1 ( NM_014413.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055228.2 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 1, mRNA.

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CloneID	OHu14846
Clone ID Related Accession (Same CDS sequence)	NM_014413.3 , NM_014413.4
Accession Version	NM_014413.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 2-alpha kinase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC341983.1, BC006524.2 and AC005995.3. On Nov 22, 2018 this sequence version replaced NM_014413.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.3633.1, SRR1803615.269101.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000199389.11/ ENSP00000199389.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGGGG GCAACTCCGG GGTCCGCAAG CGCGAAGAGG AGGGCGACGG GGCTGGGGCT 
GTGGCTGCGC CGCCGGCCAT CGACTTTCCC GCCGAGGGCC CGGACCCCGA ATATGACGAA 
TCTGATGTTC CAGCAGAAAT CCAGGTGTTA AAAGAACCCC TACAACAGCC AACCTTCCCT 
TTTGCAGTTG CAAACCAACT CTTGCTGGTT TCTTTGCTGG AGCACTTGAG CCACGTGCAT 
GAACCAAACC CACTTCGTTC AAGACAGGTG TTTAAGCTAC TTTGCCAGAC GTTTATCAAA 
ATGGGGCTGC TGTCTTCTTT CACTTGTAGT GACGAGTTTA GCTCATTGAG ACTACATCAC 
AACAGAGCTA TTACTCACTT AATGAGGTCT GCTAAAGAGA GAGTTCGTCA GGATCCTTGT 
GAGGATATTT CTCGTATCCA GAAAATCAGA TCAAGGGAAG TAGCCTTGGA AGCACAAACT 
TCACGTTACT TAAATGAATT TGAAGAACTT GCCATCTTAG GAAAAGGTGG ATACGGAAGA 
GTATACAAGG TCAGGAATAA ATTAGATGGT CAGTATTATG CAATAAAAAA AATCCTGATT 
AAGGGTGCAA CTAAAACAGT TTGCATGAAG GTCCTACGGG AAGTGAAGGT GCTGGCAGGT 
CTTCAGCACC CCAATATTGT TGGCTATCAC ACCGCGTGGA TAGAACATGT TCATGTGATT 
CAGCCACGAG CAGACAGAGC TGCCATTGAG TTGCCATCTC TGGAAGTGCT CTCCGACCAG 
GAAGAGGACA GAGAGCAATG TGGTGTTAAA AATGATGAAA GTAGCAGCTC ATCCATTATC 
TTTGCTGAGC CCACCCCAGA AAAAGAAAAA CGCTTTGGAG AATCTGACAC TGAAAATCAG 
AATAACAAGT CGGTGAAGTA CACCACCAAT TTAGTCATAA GAGAATCTGG TGAACTTGAG 
TCGACCCTGG AGCTCCAGGA AAATGGCTTG GCTGGTTTGT CTGCCAGTTC AATTGTGGAA 
CAGCAGCTGC CACTCAGGCG TAATTCCCAC CTAGAGGAGA GTTTCACATC CACCGAAGAA 
TCTTCCGAAG AAAATGTCAA CTTTTTGGGT CAGACAGAGG CACAGTACCA CCTGATGCTG 
CACATCCAGA TGCAGCTGTG TGAGCTCTCG CTGTGGGATT GGATAGTCGA GAGAAACAAG 
CGGGGCCGGG AGTATGTGGA CGAGTCTGCC TGTCCTTATG TTATGGCCAA TGTTGCAACA 
AAAATTTTTC AAGAATTGGT AGAAGGTGTG TTTTACATAC ATAACATGGG AATTGTGCAC 
CGAGATCTGA AGCCAAGAAA TATTTTTCTT CATGGCCCTG ATCAGCAAGT AAAAATAGGA 
GACTTTGGTC TGGCCTGCAC AGACATCCTA CAGAAGAACA CAGACTGGAC CAACAGAAAC 
GGGAAGAGAA CACCAACACA TACGTCCAGA GTGGGTACTT GTCTGTACGC TTCACCCGAA 
CAGTTGGAAG GATCTGAGTA TGATGCCAAG TCAGATATGT ACAGCTTGGG TGTGGTCCTG 
CTAGAGCTCT TTCAGCCGTT TGGAACAGAA ATGGAGCGAG CAGAAGTTCT AACAGGTTTA 
AGAACTGGTC AGTTGCCGGA ATCCCTCCGT AAAAGGTGTC CAGTGCAAGC CAAGTATATC 
CAGCACTTAA CGAGAAGGAA CTCATCGCAG AGACCATCTG CCATTCAGCT GCTGCAGAGT 
GAACTTTTCC AAAATTCTGG AAATGTTAAC CTCACCCTAC AGATGAAGAT AATAGAGCAA 
GAAAAAGAAA TTGCAGAACT AAAGAAGCAG CTAAACCTCC TTTCTCAAGA CAAAGGGGTG 
AGGGATGACG GAAAGGATGG GGGCGTGGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055228.2
CDS	93..1985
Translation	MQGGNSGVRKREEEGDGAGAVAAPPAIDFPAEGPDPEYDESDVPAEIQVLKEPLQQPTFPFAVANQLLLVSLLEHLSHVHEPNPLRSRQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCEDISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSIIFAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

Target ORF information:

RefSeq Version	NM_014413.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014413.4

ORF Insert Sequence:

ATGCAGGGGG GCAACTCCGG GGTCCGCAAG CGCGAAGAGG AGGGCGACGG GGCTGGGGCT 
GTGGCTGCGC CGCCGGCCAT CGACTTTCCC GCCGAGGGCC CGGACCCCGA ATATGACGAA 
TCTGATGTTC CAGCAGAAAT CCAGGTGTTA AAAGAACCCC TACAACAGCC AACCTTCCCT 
TTTGCAGTTG CAAACCAACT CTTGCTGGTT TCTTTGCTGG AGCACTTGAG CCACGTGCAT 
GAACCAAACC CACTTCGTTC AAGACAGGTG TTTAAGCTAC TTTGCCAGAC GTTTATCAAA 
ATGGGGCTGC TGTCTTCTTT CACTTGTAGT GACGAGTTTA GCTCATTGAG ACTACATCAC 
AACAGAGCTA TTACTCACTT AATGAGGTCT GCTAAAGAGA GAGTTCGTCA GGATCCTTGT 
GAGGATATTT CTCGTATCCA GAAAATCAGA TCAAGGGAAG TAGCCTTGGA AGCACAAACT 
TCACGTTACT TAAATGAATT TGAAGAACTT GCCATCTTAG GAAAAGGTGG ATACGGAAGA 
GTATACAAGG TCAGGAATAA ATTAGATGGT CAGTATTATG CAATAAAAAA AATCCTGATT 
AAGGGTGCAA CTAAAACAGT TTGCATGAAG GTCCTACGGG AAGTGAAGGT GCTGGCAGGT 
CTTCAGCACC CCAATATTGT TGGCTATCAC ACCGCGTGGA TAGAACATGT TCATGTGATT 
CAGCCACGAG CAGACAGAGC TGCCATTGAG TTGCCATCTC TGGAAGTGCT CTCCGACCAG 
GAAGAGGACA GAGAGCAATG TGGTGTTAAA AATGATGAAA GTAGCAGCTC ATCCATTATC 
TTTGCTGAGC CCACCCCAGA AAAAGAAAAA CGCTTTGGAG AATCTGACAC TGAAAATCAG 
AATAACAAGT CGGTGAAGTA CACCACCAAT TTAGTCATAA GAGAATCTGG TGAACTTGAG 
TCGACCCTGG AGCTCCAGGA AAATGGCTTG GCTGGTTTGT CTGCCAGTTC AATTGTGGAA 
CAGCAGCTGC CACTCAGGCG TAATTCCCAC CTAGAGGAGA GTTTCACATC CACCGAAGAA 
TCTTCCGAAG AAAATGTCAA CTTTTTGGGT CAGACAGAGG CACAGTACCA CCTGATGCTG 
CACATCCAGA TGCAGCTGTG TGAGCTCTCG CTGTGGGATT GGATAGTCGA GAGAAACAAG 
CGGGGCCGGG AGTATGTGGA CGAGTCTGCC TGTCCTTATG TTATGGCCAA TGTTGCAACA 
AAAATTTTTC AAGAATTGGT AGAAGGTGTG TTTTACATAC ATAACATGGG AATTGTGCAC 
CGAGATCTGA AGCCAAGAAA TATTTTTCTT CATGGCCCTG ATCAGCAAGT AAAAATAGGA 
GACTTTGGTC TGGCCTGCAC AGACATCCTA CAGAAGAACA CAGACTGGAC CAACAGAAAC 
GGGAAGAGAA CACCAACACA TACGTCCAGA GTGGGTACTT GTCTGTACGC TTCACCCGAA 
CAGTTGGAAG GATCTGAGTA TGATGCCAAG TCAGATATGT ACAGCTTGGG TGTGGTCCTG 
CTAGAGCTCT TTCAGCCGTT TGGAACAGAA ATGGAGCGAG CAGAAGTTCT AACAGGTTTA 
AGAACTGGTC AGTTGCCGGA ATCCCTCCGT AAAAGGTGTC CAGTGCAAGC CAAGTATATC 
CAGCACTTAA CGAGAAGGAA CTCATCGCAG AGACCATCTG CCATTCAGCT GCTGCAGAGT 
GAACTTTTCC AAAATTCTGG AAATGTTAAC CTCACCCTAC AGATGAAGAT AATAGAGCAA 
GAAAAAGAAA TTGCAGAACT AAAGAAGCAG CTAAACCTCC TTTCTCAAGA CAAAGGGGTG 
AGGGATGACG GAAAGGATGG GGGCGTGGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.
Science (New York, N.Y.)365(6448)(2019 07)
Abdel-Nour M,Carneiro LAM,Downey J,Tsalikis J,Outlioua A,Prescott D,Da Costa LS,Hovingh ES,Farahvash A,Gaudet RG,Molinaro R,van Dalen R,Lau CCY,Azimi FC,Escalante NK,Trotman-Grant A,Lee JE,Gray-Owen SD,Divangahi M,Chen JJ,Philpott DJ,Arnoult D,Girardin SE

Activation of HRI is mediated by Hsp90 during stress through modulation of the HRI-Hsp90 complex.
International journal of biological macromolecules118(Pt B)1604-1613(2018 Oct)
Berwal SK,Bhatia V,Bendre A,Suresh CG,Chatterjee S,Pal JK

Domain-focused CRISPR screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells.
Science (New York, N.Y.)361(6399)285-290(2018 07)
Grevet JD,Lan X,Hamagami N,Edwards CR,Sankaranarayanan L,Ji X,Bhardwaj SK,Face CJ,Posocco DF,Abdulmalik O,Keller CA,Giardine B,Sidoli S,Garcia BA,Chou ST,Liebhaber SA,Hardison RC,Shi J,Blobel GA

An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Nature communications9(1)1188(2018 03)
Liu X,Salokas K,Tamene F,Jiu Y,Weldatsadik RG,Öhman T,Varjosalo M

Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1.
Journal of virology91(5)(2017 03)
Vincent HA,Ziehr B,Moorman NJ

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EIF2AK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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