COL8A1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL8A1B
Entrez Gene ID	562442
Full Name	collagen, type VIII, alpha 1b
Synonyms	si:ch73-162i18.4
General protein information	Preferred Names collagen, type VIII, alpha 1b Names collagen alpha-1(VIII) chain col8a2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161783.4	XP_005161840.1	collagen alpha-1(VIII) chain
XM_685822.9	XP_690914.3	collagen alpha-1(VIII) chain
XM_685822.8	XP_690914.3	collagen alpha-1(VIII) chain
XM_005161783.3	XP_005161840.1	collagen alpha-1(VIII) chain

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Col8a1	NP_031765.2
Gallus gallus (chicken)	COL8A1	XP_004938297.1
Macaca mulatta (Rhesus monkey)	COL8A1	XP_002802671.1
Bos taurus (cattle)	COL8A1	NP_001094646.1
Danio rerio (zebrafish)	col8a1b	XP_690914.3
Homo sapiens (human)	COL8A1	NP_001841.2
Pan troglodytes (chimpanzee)	COL8A1	XP_001142044.1
Rattus norvegicus (Norway rat)	Col8a1	NP_001100570.1
Xenopus tropicalis (tropical clawed frog)	col8a1	XP_002941034.1
Canis lupus familiaris (dog)	COL8A1	XP_545072.3

Gene Ontology

The following COL8A1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL8A1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa27098	XM_685822.9 Latest version!	Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa27098	XM_005161783.4 Latest version!	Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa27098	XM_685822.8	Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa27098	XM_005161783.3	Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL8A1B ( XM_005161783.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005161840.1 Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27098
Clone ID Related Accession (Same CDS sequence)	XM_005161783.4 , XM_685822.9 , XM_685822.8 , XM_005161783.3
Accession Version	XM_005161783.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(VIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161783.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGC CTCTCCTCTC TGCCTGCTTG TTTGTACTGG TGGAGCATTT GTGCCTGCTA 
CATCATATCC ATGCGGGGGC ATTTTATGGA CACAAACCAT TGCCTCAAAA GCGCCAGCCT 
CTGCCACAGT TACCTCTTCT ACCACATGGC AATGAAGGAA TAACACAACA CCATTACCAT 
GGAAAGGAAA TCCCTCAACT TCCCTTTGGA AATGAGATGC CTTTGCTTCC ACAGTATGGG 
AAGGAACGTC CACAGTTTCC CATGCATATG GGCATACCGA TTGCAAGAAA AGGTGAAACA 
GTTCCAAGAG GACAGAAAGG TCCTCCTGGA GAGCCCGGTC CGATAGGGCT TCAAGGGCCT 
CGGGGCCCTC CGGGACTTCC TGGTCATGGT ATCCCAGGGC CTACAGGTAA ACCTGGTCCT 
CAGGGCCCAC CAGGCCTTCC TGGAATTGGA AAGCCAGGTA TGGCAGGTTT GCCCGGAAAA 
CCAGGAGGTA TAGGTTTACC AGGGCCAAAG GGTGAGTTAG GACCAATTGG TGGTGAAGGA 
CCAATAGGAC TACCAGGACC TCCAGGTCTT CCGGGGCCAC ATGGCTTGCC AGGTGTGGCG 
AAACCAGGGG GACAAGGACT CCCTGGACAA CCTGGCCCTC GGGGTGAACC TGGACAAAAG 
GGCTTCCCTG GCATCCCTGG CCTCCAAGGG CCAAAAGGTG AGAATGGTTT TGGTCTCCCA 
GGATTGCCAG GCGTAAAGGG ACTTCCAGGA TTGCCTGGAC CACCAGGACA AGTTGGACTA 
CCAGGAATTG GCAAACCAGG ACTGAATGGA CTACCTGGGG TTCCTGGGGG GCAAGGTAAA 
CCTGGTGCAC CTGGAGAACC AGGGCTAGCT GGAGTACCTG GGGAGAGAGG ACAAATTGGA 
CCACCGGGAT TGCCAGGCTT TGGCAAACCA GGTTTAGATG GCTTACCAGG GAAACCAGGT 
CTACCAGGTG GAAGGGGCGA GCCAGGTCAA ACGGGTTTAA CTGGGGGTCC AGGCTTGCCT 
GGATTTGGCA AGCCTGGTTA TCCTGGCCCT AAAGGTGAAA AAGGGCATGG TGGATTGCCT 
GGGCCAATAG GCCCAAAAGG TGAAAAAGGT CATGGTGGTC TACCTGGAAT GATTGGACTG 
CAAGGGCCAA GTGGCCTCCC AGGTCCTCCA GGTCTTATGG GACCCCCTGG AGGCATTGGA 
TTTCCTGGTC CAAAAGGAGA AGGTGGTGCA GTGGGACCAA AAGGACTATT GGGTCCAAAA 
GGTGAACCAG GACCTCAAGG TCTTCCTGGC AAGCCAGGAT TCCCAGGAGA ATTGGGGCAA 
CCAGGACCTA GAGGTTTAAC TGGTCCCTTA GGACCAAAAG GTGAAAGTGG ACACAAAGGC 
TTACCCGGGC TGCCTGGAGT GCCTGGAAAG CCTGGTACAA AAGGAGAACT TGGATTTCCA 
GGTGAGAAAG GTCATCCAGG GCCTATGGGA ATACCAGGAT TAATAGGTCC AGCAGGACCA 
ATAGGCCCTC CTGGTCTACC TGGTCCAAAA GGAGAATATG GACCACCAGG AAAACCTGGA 
ATCCCAGGTG AAGGAAAACC AGGATTTCCA GGCCCAACAG GTCCTGCTGG TATACCTGGA 
CCGAGTGGGC CCCATGGTCC GCCTGGAATA CCTGGTCCTC CTGGCCCACC TGGGCTACCT 
GGACCTCGTG CTCAAGCTTC ATTTTTTGGG CAAATACTTC CTGAGACAGG TCCTGGGCTT 
GATGGGGAAA AAGGTGGTTA TAAGAAAGAA AAGCCTGGAG GAGCTATTAT TGGTAGGCAT 
GGCCTAGAAA TGCCTGCATT TACAGCTCAG CTGACAACCC CATTTCCCCA AGTTGGACAA 
CCTGTTGTCT TCAACAAAAT TTTGCATAAT GGAAACCAAA ATTACAACTT GCAAACTGGC 
ATCTTCACAT GCAAAATACC TGGAATTTAC TACTTTGTCT ACCATGTTAA TTGCAAAGGA 
AACAATGTAT GGGTGGGTTT ATACAGAAAC AATGAGCCAG TTATGTATAC ATATGATGAA 
TACAAGAAAA GTTACCTGGA TCAGGCATCA GGGAGTGCTG TACTGCCCTT AAACTTTGGA 
GACACTGTTC ACGTTCAGTT ACCATCAGAG CAAGCAGCAG GACTTTATGC TGGTCCTTAC 
GTCCACTCAT CATTCTCTGG GTATTTGTTA TATCCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161840.1
CDS	141..2342
Translation	MAGPLLSACLFVLVEHLCLLHHIHAGAFYGHKPLPQKRQPLPQLPLLPHGNEGITQHHYHGKEIPQLPFGNEMPLLPQYGKERPQFPMHMGIPIARKGETVPRGQKGPPGEPGPIGLQGPRGPPGLPGHGIPGPTGKPGPQGPPGLPGIGKPGMAGLPGKPGGIGLPGPKGELGPIGGEGPIGLPGPPGLPGPHGLPGVAKPGGQGLPGQPGPRGEPGQKGFPGIPGLQGPKGENGFGLPGLPGVKGLPGLPGPPGQVGLPGIGKPGLNGLPGVPGGQGKPGAPGEPGLAGVPGERGQIGPPGLPGFGKPGLDGLPGKPGLPGGRGEPGQTGLTGGPGLPGFGKPGYPGPKGEKGHGGLPGPIGPKGEKGHGGLPGMIGLQGPSGLPGPPGLMGPPGGIGFPGPKGEGGAVGPKGLLGPKGEPGPQGLPGKPGFPGELGQPGPRGLTGPLGPKGESGHKGLPGLPGVPGKPGTKGELGFPGEKGHPGPMGIPGLIGPAGPIGPPGLPGPKGEYGPPGKPGIPGEGKPGFPGPTGPAGIPGPSGPHGPPGIPGPPGPPGLPGPRAQASFFGQILPETGPGLDGEKGGYKKEKPGGAIIGRHGLEMPAFTAQLTTPFPQVGQPVVFNKILHNGNQNYNLQTGIFTCKIPGIYYFVYHVNCKGNNVWVGLYRNNEPVMYTYDEYKKSYLDQASGSAVLPLNFGDTVHVQLPSEQAAGLYAGPYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	XM_005161783.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161783.4

ORF Insert Sequence:

ATGGCTGGGC CTCTCCTCTC TGCCTGCTTG TTTGTACTGG TGGAGCATTT GTGCCTGCTA 
CATCATATCC ATGCGGGGGC ATTTTATGGA CACAAACCAT TGCCTCAAAA GCGCCAGCCT 
CTGCCACAGT TACCTCTTCT ACCACATGGC AATGAAGGAA TAACACAACA CCATTACCAT 
GGAAAGGAAA TCCCTCAACT TCCCTTTGGA AATGAGATGC CTTTGCTTCC ACAGTATGGG 
AAGGAACGTC CACAGTTTCC CATGCATATG GGCATACCGA TTGCAAGAAA AGGTGAAACA 
GTTCCAAGAG GACAGAAAGG TCCTCCTGGA GAGCCCGGTC CGATAGGGCT TCAAGGGCCT 
CGGGGCCCTC CGGGACTTCC TGGTCATGGT ATCCCAGGGC CTACAGGTAA ACCTGGTCCT 
CAGGGCCCAC CAGGCCTTCC TGGAATTGGA AAGCCAGGTA TGGCAGGTTT GCCCGGAAAA 
CCAGGAGGTA TAGGTTTACC AGGGCCAAAG GGTGAGTTAG GACCAATTGG TGGTGAAGGA 
CCAATAGGAC TACCAGGACC TCCAGGTCTT CCGGGGCCAC ATGGCTTGCC AGGTGTGGCG 
AAACCAGGGG GACAAGGACT CCCTGGACAA CCTGGCCCTC GGGGTGAACC TGGACAAAAG 
GGCTTCCCTG GCATCCCTGG CCTCCAAGGG CCAAAAGGTG AGAATGGTTT TGGTCTCCCA 
GGATTGCCAG GCGTAAAGGG ACTTCCAGGA TTGCCTGGAC CACCAGGACA AGTTGGACTA 
CCAGGAATTG GCAAACCAGG ACTGAATGGA CTACCTGGGG TTCCTGGGGG GCAAGGTAAA 
CCTGGTGCAC CTGGAGAACC AGGGCTAGCT GGAGTACCTG GGGAGAGAGG ACAAATTGGA 
CCACCGGGAT TGCCAGGCTT TGGCAAACCA GGTTTAGATG GCTTACCAGG GAAACCAGGT 
CTACCAGGTG GAAGGGGCGA GCCAGGTCAA ACGGGTTTAA CTGGGGGTCC AGGCTTGCCT 
GGATTTGGCA AGCCTGGTTA TCCTGGCCCT AAAGGTGAAA AAGGGCATGG TGGATTGCCT 
GGGCCAATAG GCCCAAAAGG TGAAAAAGGT CATGGTGGTC TACCTGGAAT GATTGGACTG 
CAAGGGCCAA GTGGCCTCCC AGGTCCTCCA GGTCTTATGG GACCCCCTGG AGGCATTGGA 
TTTCCTGGTC CAAAAGGAGA AGGTGGTGCA GTGGGACCAA AAGGACTATT GGGTCCAAAA 
GGTGAACCAG GACCTCAAGG TCTTCCTGGC AAGCCAGGAT TCCCAGGAGA ATTGGGGCAA 
CCAGGACCTA GAGGTTTAAC TGGTCCCTTA GGACCAAAAG GTGAAAGTGG ACACAAAGGC 
TTACCCGGGC TGCCTGGAGT GCCTGGAAAG CCTGGTACAA AAGGAGAACT TGGATTTCCA 
GGTGAGAAAG GTCATCCAGG GCCTATGGGA ATACCAGGAT TAATAGGTCC AGCAGGACCA 
ATAGGCCCTC CTGGTCTACC TGGTCCAAAA GGAGAATATG GACCACCAGG AAAACCTGGA 
ATCCCAGGTG AAGGAAAACC AGGATTTCCA GGCCCAACAG GTCCTGCTGG TATACCTGGA 
CCGAGTGGGC CCCATGGTCC GCCTGGAATA CCTGGTCCTC CTGGCCCACC TGGGCTACCT 
GGACCTCGTG CTCAAGCTTC ATTTTTTGGG CAAATACTTC CTGAGACAGG TCCTGGGCTT 
GATGGGGAAA AAGGTGGTTA TAAGAAAGAA AAGCCTGGAG GAGCTATTAT TGGTAGGCAT 
GGCCTAGAAA TGCCTGCATT TACAGCTCAG CTGACAACCC CATTTCCCCA AGTTGGACAA 
CCTGTTGTCT TCAACAAAAT TTTGCATAAT GGAAACCAAA ATTACAACTT GCAAACTGGC 
ATCTTCACAT GCAAAATACC TGGAATTTAC TACTTTGTCT ACCATGTTAA TTGCAAAGGA 
AACAATGTAT GGGTGGGTTT ATACAGAAAC AATGAGCCAG TTATGTATAC ATATGATGAA 
TACAAGAAAA GTTACCTGGA TCAGGCATCA GGGAGTGCTG TACTGCCCTT AAACTTTGGA 
GACACTGTTC ACGTTCAGTT ACCATCAGAG CAAGCAGCAG GACTTTATGC TGGTCCTTAC 
GTCCACTCAT CATTCTCTGG GTATTTGTTA TATCCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A1B ( XM_685822.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_690914.3 Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27098
Clone ID Related Accession (Same CDS sequence)	XM_005161783.4 , XM_685822.9 , XM_685822.8 , XM_005161783.3
Accession Version	XM_685822.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	collagen alpha-1(VIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685822.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGC CTCTCCTCTC TGCCTGCTTG TTTGTACTGG TGGAGCATTT GTGCCTGCTA 
CATCATATCC ATGCGGGGGC ATTTTATGGA CACAAACCAT TGCCTCAAAA GCGCCAGCCT 
CTGCCACAGT TACCTCTTCT ACCACATGGC AATGAAGGAA TAACACAACA CCATTACCAT 
GGAAAGGAAA TCCCTCAACT TCCCTTTGGA AATGAGATGC CTTTGCTTCC ACAGTATGGG 
AAGGAACGTC CACAGTTTCC CATGCATATG GGCATACCGA TTGCAAGAAA AGGTGAAACA 
GTTCCAAGAG GACAGAAAGG TCCTCCTGGA GAGCCCGGTC CGATAGGGCT TCAAGGGCCT 
CGGGGCCCTC CGGGACTTCC TGGTCATGGT ATCCCAGGGC CTACAGGTAA ACCTGGTCCT 
CAGGGCCCAC CAGGCCTTCC TGGAATTGGA AAGCCAGGTA TGGCAGGTTT GCCCGGAAAA 
CCAGGAGGTA TAGGTTTACC AGGGCCAAAG GGTGAGTTAG GACCAATTGG TGGTGAAGGA 
CCAATAGGAC TACCAGGACC TCCAGGTCTT CCGGGGCCAC ATGGCTTGCC AGGTGTGGCG 
AAACCAGGGG GACAAGGACT CCCTGGACAA CCTGGCCCTC GGGGTGAACC TGGACAAAAG 
GGCTTCCCTG GCATCCCTGG CCTCCAAGGG CCAAAAGGTG AGAATGGTTT TGGTCTCCCA 
GGATTGCCAG GCGTAAAGGG ACTTCCAGGA TTGCCTGGAC CACCAGGACA AGTTGGACTA 
CCAGGAATTG GCAAACCAGG ACTGAATGGA CTACCTGGGG TTCCTGGGGG GCAAGGTAAA 
CCTGGTGCAC CTGGAGAACC AGGGCTAGCT GGAGTACCTG GGGAGAGAGG ACAAATTGGA 
CCACCGGGAT TGCCAGGCTT TGGCAAACCA GGTTTAGATG GCTTACCAGG GAAACCAGGT 
CTACCAGGTG GAAGGGGCGA GCCAGGTCAA ACGGGTTTAA CTGGGGGTCC AGGCTTGCCT 
GGATTTGGCA AGCCTGGTTA TCCTGGCCCT AAAGGTGAAA AAGGGCATGG TGGATTGCCT 
GGGCCAATAG GCCCAAAAGG TGAAAAAGGT CATGGTGGTC TACCTGGAAT GATTGGACTG 
CAAGGGCCAA GTGGCCTCCC AGGTCCTCCA GGTCTTATGG GACCCCCTGG AGGCATTGGA 
TTTCCTGGTC CAAAAGGAGA AGGTGGTGCA GTGGGACCAA AAGGACTATT GGGTCCAAAA 
GGTGAACCAG GACCTCAAGG TCTTCCTGGC AAGCCAGGAT TCCCAGGAGA ATTGGGGCAA 
CCAGGACCTA GAGGTTTAAC TGGTCCCTTA GGACCAAAAG GTGAAAGTGG ACACAAAGGC 
TTACCCGGGC TGCCTGGAGT GCCTGGAAAG CCTGGTACAA AAGGAGAACT TGGATTTCCA 
GGTGAGAAAG GTCATCCAGG GCCTATGGGA ATACCAGGAT TAATAGGTCC AGCAGGACCA 
ATAGGCCCTC CTGGTCTACC TGGTCCAAAA GGAGAATATG GACCACCAGG AAAACCTGGA 
ATCCCAGGTG AAGGAAAACC AGGATTTCCA GGCCCAACAG GTCCTGCTGG TATACCTGGA 
CCGAGTGGGC CCCATGGTCC GCCTGGAATA CCTGGTCCTC CTGGCCCACC TGGGCTACCT 
GGACCTCGTG CTCAAGCTTC ATTTTTTGGG CAAATACTTC CTGAGACAGG TCCTGGGCTT 
GATGGGGAAA AAGGTGGTTA TAAGAAAGAA AAGCCTGGAG GAGCTATTAT TGGTAGGCAT 
GGCCTAGAAA TGCCTGCATT TACAGCTCAG CTGACAACCC CATTTCCCCA AGTTGGACAA 
CCTGTTGTCT TCAACAAAAT TTTGCATAAT GGAAACCAAA ATTACAACTT GCAAACTGGC 
ATCTTCACAT GCAAAATACC TGGAATTTAC TACTTTGTCT ACCATGTTAA TTGCAAAGGA 
AACAATGTAT GGGTGGGTTT ATACAGAAAC AATGAGCCAG TTATGTATAC ATATGATGAA 
TACAAGAAAA GTTACCTGGA TCAGGCATCA GGGAGTGCTG TACTGCCCTT AAACTTTGGA 
GACACTGTTC ACGTTCAGTT ACCATCAGAG CAAGCAGCAG GACTTTATGC TGGTCCTTAC 
GTCCACTCAT CATTCTCTGG GTATTTGTTA TATCCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690914.3
CDS	101..2302
Translation	MAGPLLSACLFVLVEHLCLLHHIHAGAFYGHKPLPQKRQPLPQLPLLPHGNEGITQHHYHGKEIPQLPFGNEMPLLPQYGKERPQFPMHMGIPIARKGETVPRGQKGPPGEPGPIGLQGPRGPPGLPGHGIPGPTGKPGPQGPPGLPGIGKPGMAGLPGKPGGIGLPGPKGELGPIGGEGPIGLPGPPGLPGPHGLPGVAKPGGQGLPGQPGPRGEPGQKGFPGIPGLQGPKGENGFGLPGLPGVKGLPGLPGPPGQVGLPGIGKPGLNGLPGVPGGQGKPGAPGEPGLAGVPGERGQIGPPGLPGFGKPGLDGLPGKPGLPGGRGEPGQTGLTGGPGLPGFGKPGYPGPKGEKGHGGLPGPIGPKGEKGHGGLPGMIGLQGPSGLPGPPGLMGPPGGIGFPGPKGEGGAVGPKGLLGPKGEPGPQGLPGKPGFPGELGQPGPRGLTGPLGPKGESGHKGLPGLPGVPGKPGTKGELGFPGEKGHPGPMGIPGLIGPAGPIGPPGLPGPKGEYGPPGKPGIPGEGKPGFPGPTGPAGIPGPSGPHGPPGIPGPPGPPGLPGPRAQASFFGQILPETGPGLDGEKGGYKKEKPGGAIIGRHGLEMPAFTAQLTTPFPQVGQPVVFNKILHNGNQNYNLQTGIFTCKIPGIYYFVYHVNCKGNNVWVGLYRNNEPVMYTYDEYKKSYLDQASGSAVLPLNFGDTVHVQLPSEQAAGLYAGPYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	XM_685822.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685822.9

ORF Insert Sequence:

ATGGCTGGGC CTCTCCTCTC TGCCTGCTTG TTTGTACTGG TGGAGCATTT GTGCCTGCTA 
CATCATATCC ATGCGGGGGC ATTTTATGGA CACAAACCAT TGCCTCAAAA GCGCCAGCCT 
CTGCCACAGT TACCTCTTCT ACCACATGGC AATGAAGGAA TAACACAACA CCATTACCAT 
GGAAAGGAAA TCCCTCAACT TCCCTTTGGA AATGAGATGC CTTTGCTTCC ACAGTATGGG 
AAGGAACGTC CACAGTTTCC CATGCATATG GGCATACCGA TTGCAAGAAA AGGTGAAACA 
GTTCCAAGAG GACAGAAAGG TCCTCCTGGA GAGCCCGGTC CGATAGGGCT TCAAGGGCCT 
CGGGGCCCTC CGGGACTTCC TGGTCATGGT ATCCCAGGGC CTACAGGTAA ACCTGGTCCT 
CAGGGCCCAC CAGGCCTTCC TGGAATTGGA AAGCCAGGTA TGGCAGGTTT GCCCGGAAAA 
CCAGGAGGTA TAGGTTTACC AGGGCCAAAG GGTGAGTTAG GACCAATTGG TGGTGAAGGA 
CCAATAGGAC TACCAGGACC TCCAGGTCTT CCGGGGCCAC ATGGCTTGCC AGGTGTGGCG 
AAACCAGGGG GACAAGGACT CCCTGGACAA CCTGGCCCTC GGGGTGAACC TGGACAAAAG 
GGCTTCCCTG GCATCCCTGG CCTCCAAGGG CCAAAAGGTG AGAATGGTTT TGGTCTCCCA 
GGATTGCCAG GCGTAAAGGG ACTTCCAGGA TTGCCTGGAC CACCAGGACA AGTTGGACTA 
CCAGGAATTG GCAAACCAGG ACTGAATGGA CTACCTGGGG TTCCTGGGGG GCAAGGTAAA 
CCTGGTGCAC CTGGAGAACC AGGGCTAGCT GGAGTACCTG GGGAGAGAGG ACAAATTGGA 
CCACCGGGAT TGCCAGGCTT TGGCAAACCA GGTTTAGATG GCTTACCAGG GAAACCAGGT 
CTACCAGGTG GAAGGGGCGA GCCAGGTCAA ACGGGTTTAA CTGGGGGTCC AGGCTTGCCT 
GGATTTGGCA AGCCTGGTTA TCCTGGCCCT AAAGGTGAAA AAGGGCATGG TGGATTGCCT 
GGGCCAATAG GCCCAAAAGG TGAAAAAGGT CATGGTGGTC TACCTGGAAT GATTGGACTG 
CAAGGGCCAA GTGGCCTCCC AGGTCCTCCA GGTCTTATGG GACCCCCTGG AGGCATTGGA 
TTTCCTGGTC CAAAAGGAGA AGGTGGTGCA GTGGGACCAA AAGGACTATT GGGTCCAAAA 
GGTGAACCAG GACCTCAAGG TCTTCCTGGC AAGCCAGGAT TCCCAGGAGA ATTGGGGCAA 
CCAGGACCTA GAGGTTTAAC TGGTCCCTTA GGACCAAAAG GTGAAAGTGG ACACAAAGGC 
TTACCCGGGC TGCCTGGAGT GCCTGGAAAG CCTGGTACAA AAGGAGAACT TGGATTTCCA 
GGTGAGAAAG GTCATCCAGG GCCTATGGGA ATACCAGGAT TAATAGGTCC AGCAGGACCA 
ATAGGCCCTC CTGGTCTACC TGGTCCAAAA GGAGAATATG GACCACCAGG AAAACCTGGA 
ATCCCAGGTG AAGGAAAACC AGGATTTCCA GGCCCAACAG GTCCTGCTGG TATACCTGGA 
CCGAGTGGGC CCCATGGTCC GCCTGGAATA CCTGGTCCTC CTGGCCCACC TGGGCTACCT 
GGACCTCGTG CTCAAGCTTC ATTTTTTGGG CAAATACTTC CTGAGACAGG TCCTGGGCTT 
GATGGGGAAA AAGGTGGTTA TAAGAAAGAA AAGCCTGGAG GAGCTATTAT TGGTAGGCAT 
GGCCTAGAAA TGCCTGCATT TACAGCTCAG CTGACAACCC CATTTCCCCA AGTTGGACAA 
CCTGTTGTCT TCAACAAAAT TTTGCATAAT GGAAACCAAA ATTACAACTT GCAAACTGGC 
ATCTTCACAT GCAAAATACC TGGAATTTAC TACTTTGTCT ACCATGTTAA TTGCAAAGGA 
AACAATGTAT GGGTGGGTTT ATACAGAAAC AATGAGCCAG TTATGTATAC ATATGATGAA 
TACAAGAAAA GTTACCTGGA TCAGGCATCA GGGAGTGCTG TACTGCCCTT AAACTTTGGA 
GACACTGTTC ACGTTCAGTT ACCATCAGAG CAAGCAGCAG GACTTTATGC TGGTCCTTAC 
GTCCACTCAT CATTCTCTGG GTATTTGTTA TATCCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A1B ( XM_685822.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_690914.3 Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27098
Clone ID Related Accession (Same CDS sequence)	XM_005161783.4 , XM_685822.9 , XM_685822.8 , XM_005161783.3
Accession Version	XM_685822.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(VIII) chain
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685822.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGC CTCTCCTCTC TGCCTGCTTG TTTGTACTGG TGGAGCATTT GTGCCTGCTA 
CATCATATCC ATGCGGGGGC ATTTTATGGA CACAAACCAT TGCCTCAAAA GCGCCAGCCT 
CTGCCACAGT TACCTCTTCT ACCACATGGC AATGAAGGAA TAACACAACA CCATTACCAT 
GGAAAGGAAA TCCCTCAACT TCCCTTTGGA AATGAGATGC CTTTGCTTCC ACAGTATGGG 
AAGGAACGTC CACAGTTTCC CATGCATATG GGCATACCGA TTGCAAGAAA AGGTGAAACA 
GTTCCAAGAG GACAGAAAGG TCCTCCTGGA GAGCCCGGTC CGATAGGGCT TCAAGGGCCT 
CGGGGCCCTC CGGGACTTCC TGGTCATGGT ATCCCAGGGC CTACAGGTAA ACCTGGTCCT 
CAGGGCCCAC CAGGCCTTCC TGGAATTGGA AAGCCAGGTA TGGCAGGTTT GCCCGGAAAA 
CCAGGAGGTA TAGGTTTACC AGGGCCAAAG GGTGAGTTAG GACCAATTGG TGGTGAAGGA 
CCAATAGGAC TACCAGGACC TCCAGGTCTT CCGGGGCCAC ATGGCTTGCC AGGTGTGGCG 
AAACCAGGGG GACAAGGACT CCCTGGACAA CCTGGCCCTC GGGGTGAACC TGGACAAAAG 
GGCTTCCCTG GCATCCCTGG CCTCCAAGGG CCAAAAGGTG AGAATGGTTT TGGTCTCCCA 
GGATTGCCAG GCGTAAAGGG ACTTCCAGGA TTGCCTGGAC CACCAGGACA AGTTGGACTA 
CCAGGAATTG GCAAACCAGG ACTGAATGGA CTACCTGGGG TTCCTGGGGG GCAAGGTAAA 
CCTGGTGCAC CTGGAGAACC AGGGCTAGCT GGAGTACCTG GGGAGAGAGG ACAAATTGGA 
CCACCGGGAT TGCCAGGCTT TGGCAAACCA GGTTTAGATG GCTTACCAGG GAAACCAGGT 
CTACCAGGTG GAAGGGGCGA GCCAGGTCAA ACGGGTTTAA CTGGGGGTCC AGGCTTGCCT 
GGATTTGGCA AGCCTGGTTA TCCTGGCCCT AAAGGTGAAA AAGGGCATGG TGGATTGCCT 
GGGCCAATAG GCCCAAAAGG TGAAAAAGGT CATGGTGGTC TACCTGGAAT GATTGGACTG 
CAAGGGCCAA GTGGCCTCCC AGGTCCTCCA GGTCTTATGG GACCCCCTGG AGGCATTGGA 
TTTCCTGGTC CAAAAGGAGA AGGTGGTGCA GTGGGACCAA AAGGACTATT GGGTCCAAAA 
GGTGAACCAG GACCTCAAGG TCTTCCTGGC AAGCCAGGAT TCCCAGGAGA ATTGGGGCAA 
CCAGGACCTA GAGGTTTAAC TGGTCCCTTA GGACCAAAAG GTGAAAGTGG ACACAAAGGC 
TTACCCGGGC TGCCTGGAGT GCCTGGAAAG CCTGGTACAA AAGGAGAACT TGGATTTCCA 
GGTGAGAAAG GTCATCCAGG GCCTATGGGA ATACCAGGAT TAATAGGTCC AGCAGGACCA 
ATAGGCCCTC CTGGTCTACC TGGTCCAAAA GGAGAATATG GACCACCAGG AAAACCTGGA 
ATCCCAGGTG AAGGAAAACC AGGATTTCCA GGCCCAACAG GTCCTGCTGG TATACCTGGA 
CCGAGTGGGC CCCATGGTCC GCCTGGAATA CCTGGTCCTC CTGGCCCACC TGGGCTACCT 
GGACCTCGTG CTCAAGCTTC ATTTTTTGGG CAAATACTTC CTGAGACAGG TCCTGGGCTT 
GATGGGGAAA AAGGTGGTTA TAAGAAAGAA AAGCCTGGAG GAGCTATTAT TGGTAGGCAT 
GGCCTAGAAA TGCCTGCATT TACAGCTCAG CTGACAACCC CATTTCCCCA AGTTGGACAA 
CCTGTTGTCT TCAACAAAAT TTTGCATAAT GGAAACCAAA ATTACAACTT GCAAACTGGC 
ATCTTCACAT GCAAAATACC TGGAATTTAC TACTTTGTCT ACCATGTTAA TTGCAAAGGA 
AACAATGTAT GGGTGGGTTT ATACAGAAAC AATGAGCCAG TTATGTATAC ATATGATGAA 
TACAAGAAAA GTTACCTGGA TCAGGCATCA GGGAGTGCTG TACTGCCCTT AAACTTTGGA 
GACACTGTTC ACGTTCAGTT ACCATCAGAG CAAGCAGCAG GACTTTATGC TGGTCCTTAC 
GTCCACTCAT CATTCTCTGG GTATTTGTTA TATCCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690914.3
CDS	277..2478
Translation	MAGPLLSACLFVLVEHLCLLHHIHAGAFYGHKPLPQKRQPLPQLPLLPHGNEGITQHHYHGKEIPQLPFGNEMPLLPQYGKERPQFPMHMGIPIARKGETVPRGQKGPPGEPGPIGLQGPRGPPGLPGHGIPGPTGKPGPQGPPGLPGIGKPGMAGLPGKPGGIGLPGPKGELGPIGGEGPIGLPGPPGLPGPHGLPGVAKPGGQGLPGQPGPRGEPGQKGFPGIPGLQGPKGENGFGLPGLPGVKGLPGLPGPPGQVGLPGIGKPGLNGLPGVPGGQGKPGAPGEPGLAGVPGERGQIGPPGLPGFGKPGLDGLPGKPGLPGGRGEPGQTGLTGGPGLPGFGKPGYPGPKGEKGHGGLPGPIGPKGEKGHGGLPGMIGLQGPSGLPGPPGLMGPPGGIGFPGPKGEGGAVGPKGLLGPKGEPGPQGLPGKPGFPGELGQPGPRGLTGPLGPKGESGHKGLPGLPGVPGKPGTKGELGFPGEKGHPGPMGIPGLIGPAGPIGPPGLPGPKGEYGPPGKPGIPGEGKPGFPGPTGPAGIPGPSGPHGPPGIPGPPGPPGLPGPRAQASFFGQILPETGPGLDGEKGGYKKEKPGGAIIGRHGLEMPAFTAQLTTPFPQVGQPVVFNKILHNGNQNYNLQTGIFTCKIPGIYYFVYHVNCKGNNVWVGLYRNNEPVMYTYDEYKKSYLDQASGSAVLPLNFGDTVHVQLPSEQAAGLYAGPYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	XM_685822.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685822.8

ORF Insert Sequence:

ATGGCTGGGC CTCTCCTCTC TGCCTGCTTG TTTGTACTGG TGGAGCATTT GTGCCTGCTA 
CATCATATCC ATGCGGGGGC ATTTTATGGA CACAAACCAT TGCCTCAAAA GCGCCAGCCT 
CTGCCACAGT TACCTCTTCT ACCACATGGC AATGAAGGAA TAACACAACA CCATTACCAT 
GGAAAGGAAA TCCCTCAACT TCCCTTTGGA AATGAGATGC CTTTGCTTCC ACAGTATGGG 
AAGGAACGTC CACAGTTTCC CATGCATATG GGCATACCGA TTGCAAGAAA AGGTGAAACA 
GTTCCAAGAG GACAGAAAGG TCCTCCTGGA GAGCCCGGTC CGATAGGGCT TCAAGGGCCT 
CGGGGCCCTC CGGGACTTCC TGGTCATGGT ATCCCAGGGC CTACAGGTAA ACCTGGTCCT 
CAGGGCCCAC CAGGCCTTCC TGGAATTGGA AAGCCAGGTA TGGCAGGTTT GCCCGGAAAA 
CCAGGAGGTA TAGGTTTACC AGGGCCAAAG GGTGAGTTAG GACCAATTGG TGGTGAAGGA 
CCAATAGGAC TACCAGGACC TCCAGGTCTT CCGGGGCCAC ATGGCTTGCC AGGTGTGGCG 
AAACCAGGGG GACAAGGACT CCCTGGACAA CCTGGCCCTC GGGGTGAACC TGGACAAAAG 
GGCTTCCCTG GCATCCCTGG CCTCCAAGGG CCAAAAGGTG AGAATGGTTT TGGTCTCCCA 
GGATTGCCAG GCGTAAAGGG ACTTCCAGGA TTGCCTGGAC CACCAGGACA AGTTGGACTA 
CCAGGAATTG GCAAACCAGG ACTGAATGGA CTACCTGGGG TTCCTGGGGG GCAAGGTAAA 
CCTGGTGCAC CTGGAGAACC AGGGCTAGCT GGAGTACCTG GGGAGAGAGG ACAAATTGGA 
CCACCGGGAT TGCCAGGCTT TGGCAAACCA GGTTTAGATG GCTTACCAGG GAAACCAGGT 
CTACCAGGTG GAAGGGGCGA GCCAGGTCAA ACGGGTTTAA CTGGGGGTCC AGGCTTGCCT 
GGATTTGGCA AGCCTGGTTA TCCTGGCCCT AAAGGTGAAA AAGGGCATGG TGGATTGCCT 
GGGCCAATAG GCCCAAAAGG TGAAAAAGGT CATGGTGGTC TACCTGGAAT GATTGGACTG 
CAAGGGCCAA GTGGCCTCCC AGGTCCTCCA GGTCTTATGG GACCCCCTGG AGGCATTGGA 
TTTCCTGGTC CAAAAGGAGA AGGTGGTGCA GTGGGACCAA AAGGACTATT GGGTCCAAAA 
GGTGAACCAG GACCTCAAGG TCTTCCTGGC AAGCCAGGAT TCCCAGGAGA ATTGGGGCAA 
CCAGGACCTA GAGGTTTAAC TGGTCCCTTA GGACCAAAAG GTGAAAGTGG ACACAAAGGC 
TTACCCGGGC TGCCTGGAGT GCCTGGAAAG CCTGGTACAA AAGGAGAACT TGGATTTCCA 
GGTGAGAAAG GTCATCCAGG GCCTATGGGA ATACCAGGAT TAATAGGTCC AGCAGGACCA 
ATAGGCCCTC CTGGTCTACC TGGTCCAAAA GGAGAATATG GACCACCAGG AAAACCTGGA 
ATCCCAGGTG AAGGAAAACC AGGATTTCCA GGCCCAACAG GTCCTGCTGG TATACCTGGA 
CCGAGTGGGC CCCATGGTCC GCCTGGAATA CCTGGTCCTC CTGGCCCACC TGGGCTACCT 
GGACCTCGTG CTCAAGCTTC ATTTTTTGGG CAAATACTTC CTGAGACAGG TCCTGGGCTT 
GATGGGGAAA AAGGTGGTTA TAAGAAAGAA AAGCCTGGAG GAGCTATTAT TGGTAGGCAT 
GGCCTAGAAA TGCCTGCATT TACAGCTCAG CTGACAACCC CATTTCCCCA AGTTGGACAA 
CCTGTTGTCT TCAACAAAAT TTTGCATAAT GGAAACCAAA ATTACAACTT GCAAACTGGC 
ATCTTCACAT GCAAAATACC TGGAATTTAC TACTTTGTCT ACCATGTTAA TTGCAAAGGA 
AACAATGTAT GGGTGGGTTT ATACAGAAAC AATGAGCCAG TTATGTATAC ATATGATGAA 
TACAAGAAAA GTTACCTGGA TCAGGCATCA GGGAGTGCTG TACTGCCCTT AAACTTTGGA 
GACACTGTTC ACGTTCAGTT ACCATCAGAG CAAGCAGCAG GACTTTATGC TGGTCCTTAC 
GTCCACTCAT CATTCTCTGG GTATTTGTTA TATCCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A1B ( XM_005161783.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005161840.1 Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X1, mRNA.

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CloneID	ODa27098
Clone ID Related Accession (Same CDS sequence)	XM_005161783.4 , XM_685822.9 , XM_685822.8 , XM_005161783.3
Accession Version	XM_005161783.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	collagen alpha-1(VIII) chain
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGC CTCTCCTCTC TGCCTGCTTG TTTGTACTGG TGGAGCATTT GTGCCTGCTA 
CATCATATCC ATGCGGGGGC ATTTTATGGA CACAAACCAT TGCCTCAAAA GCGCCAGCCT 
CTGCCACAGT TACCTCTTCT ACCACATGGC AATGAAGGAA TAACACAACA CCATTACCAT 
GGAAAGGAAA TCCCTCAACT TCCCTTTGGA AATGAGATGC CTTTGCTTCC ACAGTATGGG 
AAGGAACGTC CACAGTTTCC CATGCATATG GGCATACCGA TTGCAAGAAA AGGTGAAACA 
GTTCCAAGAG GACAGAAAGG TCCTCCTGGA GAGCCCGGTC CGATAGGGCT TCAAGGGCCT 
CGGGGCCCTC CGGGACTTCC TGGTCATGGT ATCCCAGGGC CTACAGGTAA ACCTGGTCCT 
CAGGGCCCAC CAGGCCTTCC TGGAATTGGA AAGCCAGGTA TGGCAGGTTT GCCCGGAAAA 
CCAGGAGGTA TAGGTTTACC AGGGCCAAAG GGTGAGTTAG GACCAATTGG TGGTGAAGGA 
CCAATAGGAC TACCAGGACC TCCAGGTCTT CCGGGGCCAC ATGGCTTGCC AGGTGTGGCG 
AAACCAGGGG GACAAGGACT CCCTGGACAA CCTGGCCCTC GGGGTGAACC TGGACAAAAG 
GGCTTCCCTG GCATCCCTGG CCTCCAAGGG CCAAAAGGTG AGAATGGTTT TGGTCTCCCA 
GGATTGCCAG GCGTAAAGGG ACTTCCAGGA TTGCCTGGAC CACCAGGACA AGTTGGACTA 
CCAGGAATTG GCAAACCAGG ACTGAATGGA CTACCTGGGG TTCCTGGGGG GCAAGGTAAA 
CCTGGTGCAC CTGGAGAACC AGGGCTAGCT GGAGTACCTG GGGAGAGAGG ACAAATTGGA 
CCACCGGGAT TGCCAGGCTT TGGCAAACCA GGTTTAGATG GCTTACCAGG GAAACCAGGT 
CTACCAGGTG GAAGGGGCGA GCCAGGTCAA ACGGGTTTAA CTGGGGGTCC AGGCTTGCCT 
GGATTTGGCA AGCCTGGTTA TCCTGGCCCT AAAGGTGAAA AAGGGCATGG TGGATTGCCT 
GGGCCAATAG GCCCAAAAGG TGAAAAAGGT CATGGTGGTC TACCTGGAAT GATTGGACTG 
CAAGGGCCAA GTGGCCTCCC AGGTCCTCCA GGTCTTATGG GACCCCCTGG AGGCATTGGA 
TTTCCTGGTC CAAAAGGAGA AGGTGGTGCA GTGGGACCAA AAGGACTATT GGGTCCAAAA 
GGTGAACCAG GACCTCAAGG TCTTCCTGGC AAGCCAGGAT TCCCAGGAGA ATTGGGGCAA 
CCAGGACCTA GAGGTTTAAC TGGTCCCTTA GGACCAAAAG GTGAAAGTGG ACACAAAGGC 
TTACCCGGGC TGCCTGGAGT GCCTGGAAAG CCTGGTACAA AAGGAGAACT TGGATTTCCA 
GGTGAGAAAG GTCATCCAGG GCCTATGGGA ATACCAGGAT TAATAGGTCC AGCAGGACCA 
ATAGGCCCTC CTGGTCTACC TGGTCCAAAA GGAGAATATG GACCACCAGG AAAACCTGGA 
ATCCCAGGTG AAGGAAAACC AGGATTTCCA GGCCCAACAG GTCCTGCTGG TATACCTGGA 
CCGAGTGGGC CCCATGGTCC GCCTGGAATA CCTGGTCCTC CTGGCCCACC TGGGCTACCT 
GGACCTCGTG CTCAAGCTTC ATTTTTTGGG CAAATACTTC CTGAGACAGG TCCTGGGCTT 
GATGGGGAAA AAGGTGGTTA TAAGAAAGAA AAGCCTGGAG GAGCTATTAT TGGTAGGCAT 
GGCCTAGAAA TGCCTGCATT TACAGCTCAG CTGACAACCC CATTTCCCCA AGTTGGACAA 
CCTGTTGTCT TCAACAAAAT TTTGCATAAT GGAAACCAAA ATTACAACTT GCAAACTGGC 
ATCTTCACAT GCAAAATACC TGGAATTTAC TACTTTGTCT ACCATGTTAA TTGCAAAGGA 
AACAATGTAT GGGTGGGTTT ATACAGAAAC AATGAGCCAG TTATGTATAC ATATGATGAA 
TACAAGAAAA GTTACCTGGA TCAGGCATCA GGGAGTGCTG TACTGCCCTT AAACTTTGGA 
GACACTGTTC ACGTTCAGTT ACCATCAGAG CAAGCAGCAG GACTTTATGC TGGTCCTTAC 
GTCCACTCAT CATTCTCTGG GTATTTGTTA TATCCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161840.1
CDS	309..2510
Translation	MAGPLLSACLFVLVEHLCLLHHIHAGAFYGHKPLPQKRQPLPQLPLLPHGNEGITQHHYHGKEIPQLPFGNEMPLLPQYGKERPQFPMHMGIPIARKGETVPRGQKGPPGEPGPIGLQGPRGPPGLPGHGIPGPTGKPGPQGPPGLPGIGKPGMAGLPGKPGGIGLPGPKGELGPIGGEGPIGLPGPPGLPGPHGLPGVAKPGGQGLPGQPGPRGEPGQKGFPGIPGLQGPKGENGFGLPGLPGVKGLPGLPGPPGQVGLPGIGKPGLNGLPGVPGGQGKPGAPGEPGLAGVPGERGQIGPPGLPGFGKPGLDGLPGKPGLPGGRGEPGQTGLTGGPGLPGFGKPGYPGPKGEKGHGGLPGPIGPKGEKGHGGLPGMIGLQGPSGLPGPPGLMGPPGGIGFPGPKGEGGAVGPKGLLGPKGEPGPQGLPGKPGFPGELGQPGPRGLTGPLGPKGESGHKGLPGLPGVPGKPGTKGELGFPGEKGHPGPMGIPGLIGPAGPIGPPGLPGPKGEYGPPGKPGIPGEGKPGFPGPTGPAGIPGPSGPHGPPGIPGPPGPPGLPGPRAQASFFGQILPETGPGLDGEKGGYKKEKPGGAIIGRHGLEMPAFTAQLTTPFPQVGQPVVFNKILHNGNQNYNLQTGIFTCKIPGIYYFVYHVNCKGNNVWVGLYRNNEPVMYTYDEYKKSYLDQASGSAVLPLNFGDTVHVQLPSEQAAGLYAGPYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	XM_005161783.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio collagen, type VIII, alpha 1b (col8a1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161783.3

ORF Insert Sequence:

ATGGCTGGGC CTCTCCTCTC TGCCTGCTTG TTTGTACTGG TGGAGCATTT GTGCCTGCTA 
CATCATATCC ATGCGGGGGC ATTTTATGGA CACAAACCAT TGCCTCAAAA GCGCCAGCCT 
CTGCCACAGT TACCTCTTCT ACCACATGGC AATGAAGGAA TAACACAACA CCATTACCAT 
GGAAAGGAAA TCCCTCAACT TCCCTTTGGA AATGAGATGC CTTTGCTTCC ACAGTATGGG 
AAGGAACGTC CACAGTTTCC CATGCATATG GGCATACCGA TTGCAAGAAA AGGTGAAACA 
GTTCCAAGAG GACAGAAAGG TCCTCCTGGA GAGCCCGGTC CGATAGGGCT TCAAGGGCCT 
CGGGGCCCTC CGGGACTTCC TGGTCATGGT ATCCCAGGGC CTACAGGTAA ACCTGGTCCT 
CAGGGCCCAC CAGGCCTTCC TGGAATTGGA AAGCCAGGTA TGGCAGGTTT GCCCGGAAAA 
CCAGGAGGTA TAGGTTTACC AGGGCCAAAG GGTGAGTTAG GACCAATTGG TGGTGAAGGA 
CCAATAGGAC TACCAGGACC TCCAGGTCTT CCGGGGCCAC ATGGCTTGCC AGGTGTGGCG 
AAACCAGGGG GACAAGGACT CCCTGGACAA CCTGGCCCTC GGGGTGAACC TGGACAAAAG 
GGCTTCCCTG GCATCCCTGG CCTCCAAGGG CCAAAAGGTG AGAATGGTTT TGGTCTCCCA 
GGATTGCCAG GCGTAAAGGG ACTTCCAGGA TTGCCTGGAC CACCAGGACA AGTTGGACTA 
CCAGGAATTG GCAAACCAGG ACTGAATGGA CTACCTGGGG TTCCTGGGGG GCAAGGTAAA 
CCTGGTGCAC CTGGAGAACC AGGGCTAGCT GGAGTACCTG GGGAGAGAGG ACAAATTGGA 
CCACCGGGAT TGCCAGGCTT TGGCAAACCA GGTTTAGATG GCTTACCAGG GAAACCAGGT 
CTACCAGGTG GAAGGGGCGA GCCAGGTCAA ACGGGTTTAA CTGGGGGTCC AGGCTTGCCT 
GGATTTGGCA AGCCTGGTTA TCCTGGCCCT AAAGGTGAAA AAGGGCATGG TGGATTGCCT 
GGGCCAATAG GCCCAAAAGG TGAAAAAGGT CATGGTGGTC TACCTGGAAT GATTGGACTG 
CAAGGGCCAA GTGGCCTCCC AGGTCCTCCA GGTCTTATGG GACCCCCTGG AGGCATTGGA 
TTTCCTGGTC CAAAAGGAGA AGGTGGTGCA GTGGGACCAA AAGGACTATT GGGTCCAAAA 
GGTGAACCAG GACCTCAAGG TCTTCCTGGC AAGCCAGGAT TCCCAGGAGA ATTGGGGCAA 
CCAGGACCTA GAGGTTTAAC TGGTCCCTTA GGACCAAAAG GTGAAAGTGG ACACAAAGGC 
TTACCCGGGC TGCCTGGAGT GCCTGGAAAG CCTGGTACAA AAGGAGAACT TGGATTTCCA 
GGTGAGAAAG GTCATCCAGG GCCTATGGGA ATACCAGGAT TAATAGGTCC AGCAGGACCA 
ATAGGCCCTC CTGGTCTACC TGGTCCAAAA GGAGAATATG GACCACCAGG AAAACCTGGA 
ATCCCAGGTG AAGGAAAACC AGGATTTCCA GGCCCAACAG GTCCTGCTGG TATACCTGGA 
CCGAGTGGGC CCCATGGTCC GCCTGGAATA CCTGGTCCTC CTGGCCCACC TGGGCTACCT 
GGACCTCGTG CTCAAGCTTC ATTTTTTGGG CAAATACTTC CTGAGACAGG TCCTGGGCTT 
GATGGGGAAA AAGGTGGTTA TAAGAAAGAA AAGCCTGGAG GAGCTATTAT TGGTAGGCAT 
GGCCTAGAAA TGCCTGCATT TACAGCTCAG CTGACAACCC CATTTCCCCA AGTTGGACAA 
CCTGTTGTCT TCAACAAAAT TTTGCATAAT GGAAACCAAA ATTACAACTT GCAAACTGGC 
ATCTTCACAT GCAAAATACC TGGAATTTAC TACTTTGTCT ACCATGTTAA TTGCAAAGGA 
AACAATGTAT GGGTGGGTTT ATACAGAAAC AATGAGCCAG TTATGTATAC ATATGATGAA 
TACAAGAAAA GTTACCTGGA TCAGGCATCA GGGAGTGCTG TACTGCCCTT AAACTTTGGA 
GACACTGTTC ACGTTCAGTT ACCATCAGAG CAAGCAGCAG GACTTTATGC TGGTCCTTAC 
GTCCACTCAT CATTCTCTGG GTATTTGTTA TATCCTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL8A1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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