DNA2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNA2
Entrez Gene ID	559535
Full Name	DNA replication helicase/nuclease 2
Synonyms	si:ch211-1n9.7
General protein information	Preferred Names DNA replication helicase/nuclease 2 Names DNA2-like helicase DNA replication ATP-dependent helicase/nuclease DNA2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021480834.1	XP_021336509.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
XM_682882.8	XP_687974.6	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X2
XM_021474283.1	XP_021329958.1	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
XM_682882.7	XP_687974.5	DNA replication ATP-dependent helicase/nuclease DNA2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DNA2	NP_001006497.1
Bos taurus (cattle)	DNA2	NP_001179731.2
Rattus norvegicus (Norway rat)	Dna2	XP_241671.6
Arabidopsis thaliana (thale cress)	emb2411	NP_001184943.1
Oryza sativa (rice)	Os04g0588200	NP_001053694.2
Canis lupus familiaris (dog)	DNA2	XP_005619489.1
Danio rerio (zebrafish)	dna2	XP_687974.5
Pan troglodytes (chimpanzee)	DNA2	XP_001163213.2
Mus musculus (house mouse)	Dna2	NP_796346.2
Drosophila melanogaster (fruit fly)	CG2990	NP_727386.1
Homo sapiens (human)	DNA2	NP_001073918.2
Macaca mulatta (Rhesus monkey)	DNA2	XP_002808547.1
Caenorhabditis elegans (roundworm)	dna-2	NP_496515.1
Magnaporthe oryzae (rice blast fungus)	MGG_02491	XP_003709288.1
Schizosaccharomyces pombe (fission yeast)	dna2	NP_596499.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004685	XP_307265.4
Xenopus tropicalis (tropical clawed frog)	dna2	XP_002936016.2

Gene Ontology

The following DNA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145568	XM_682882.8 Latest version!	Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa18850	XM_021480834.1 Latest version!	Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa18850	XM_021474283.1 Latest version!	Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa18850	XM_682882.7 Latest version!	Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNA2 ( XM_682882.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_687974.6 Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

Price & Availability↑

CloneID	ODa145568
Clone ID Related Accession (Same CDS sequence)	XM_682882.8
Accession Version	XM_682882.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_682882.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGT GCAAATCCAG CCGATCATCT ATGGTCAGTG GAGTTCAATC AAACATTTCT 
TCCTTTTTCC TCTCAAAGGT AGCGGTAAAG TCACCTAAAA AATGTGAAGT AGGAATTACA 
AGATCCCCTG TTTCTTTAAA GACTGGGTTT GTGAAGGACG AAAATGTTCC CTGCTCCCCA 
AACCCATCTG CTGTCCCAGA GACGCCTGAC AGTCTGAGAA TCGAGAGGCC TTCAGACACT 
TCATTTGAGA AACGCTCACC TGTTTCACGT GGTCTACATA CTAAAGGAGT CAGGGGTAAA 
CCTAGCCTGA ATGCACGAAA GCTGTTTCAG CCACAGAAAA ACAGCAGTTT AGAGTCAGAG 
GACACTTTTG AGTTCAAGAA TGGCGTAAGT CACTCAGGCT CCGTAAAGAG GTTGTTGCAC 
AGCACTGAAA ACATACAGAG TGCCAAGAAG TCCAGGACTA CTCAAGATCA GAAAATAACT 
GATTGTGGCC CAAGCTCAGC AACGGCCAGT CAAAATAAAT TTTCTGAAAC ATTGCAAAAA 
GCTTTCTCTA AGAGTCCTGA GAAAAAAATC AAAACTGAGT CTGTGAGTTT GGAGGGTAGC 
CGACTGAAGG CTGGACCTTG TTTGAAAATG AATGAAAAAT TAAATGGTTT CTCAAAAGAC 
TCAGGAAGAT CCTCTCATCA TCTGAGCTTT AGTACTAGTG AGAAGGAGAA TTCACCTACT 
TGTAATGTAA AGTCAGAGGC AGACATGGGT GCCGTTTCTG GAACAAATGA CTTTGATCTT 
GTGATGTCGT ATAGTTCTTG GATACAAAAT GGATCTACTG AAGAAAAAGG TACTTTTCTT 
AACTGTCCAA TTGCTAAGAA GAGGCCCTTA AATACATCTG AGCTGCAAGT GAAAGAACAA 
ATTACTGATG GAAAGACTTT GCCTGTACCA TTTGAGGACG ATTTTGCCGA TTTGATGGAC 
GATTGGTTTG ATGAAGGGAT GGAGCAAAAA TCTGAGGCAC CAAAACCCAA GAAGTTCAGT 
CATGAAAAAA ACAAAGGCAT ACCTGAGCAT GTTGTGCTGG CCTCTGGTGT TCATAACCGA 
TATTGGGTGC TGGATGTGCA GGACGTTACC TCGTCAAGAA GCTGCTCAGA GAAGCACTTG 
ACCATCACTT GCTCAAAGAC GAGTCAGGCC ACAGAGACCT GCATTCTCAA AGATGGCTGG 
GAAAGTACTC TGGTAACTGT AGGGGACATT ATTCACTTGG AAGGGAAGTG TGTTTCCGGC 
TTGTGGACAA TTGATAGAGA CTCTGCTTTT CTGATTCTTC TGCCTGACCT GCTGATATCT 
GGGACCAGCA TTGCCAGCAG CATTCGCTGT ATGAGGCGAG GGGTGCTGGG AGAGATGTTC 
AAGGCATTTG ATGGTGGATC CAAGCAGATG TTAAATGGTA CAATAGTTCA TGATATCTTT 
CAAAAAGCAG CCATGTCCTC AGATTTCTCC TCAGAGAAGA TACAGACATT TGCCACGGAG 
GCCTTAAGAA GCCCCAACTA CCTCGGACAA ATGTACAGTC TAAAGCTGAC TCAGGCTGAC 
ATGAGACAGG AAGTTGAAGA ATATCTTCCC TCTATATCTG AATGGGCAAA ACAATACCTC 
CACACTTCCC CACAGGCAGG ACAAAAACAG CTGACTCTAA AACTCCCTAG TGATGGGGCT 
CTGAGCAAAC AAGATGCTTC CAGTAGCGTG ATGGTTACTG ATTTTGTGGA CATTGAGGAG 
AACATTTGGT GTCCACGTTT TGGTCTGAAG GGAAAGATTG ATGTCACAGC TGGAGTTAAG 
ATTCACCGGA GAGGCAGGAA GCCCATGGAA AGAATTGTGC CACTGGAGCT GAAAACTGGC 
AAAGAGTCTA ACTCCATTGA GCATCGCAGT CAGGTTATTC TGTACACACT GATGAGCTCA 
GCTAGACGTT GTGATCCAGA GGCAGGTTTC CTTGTCTACT TGAAGACTGG CAGTCTACAT 
CCTGTTGTGG GCAATCACAT GGACAGGAGA GAGCTGATTA AAATAAGGAA CTCATTGGCC 
CATCACATTG GCAACAACCT GATCAAGGTG GATGGGAAAA GTCAAATGGC CCAATTGCCT 
GCCATTGTAT CTGACCAGCA AACTTGCAAA TATTGTCCTC AGAAAAGGAA CTGTGCAATT 
TACAACAGAG CTGTGGAGAG AGATCCTGTG GAGAAGTGCA GTGAAAATCC ACAGGCCTTT 
GTGCAGTCAG AGAGTGAACA CCTCAGTCAG GTGCACCTCC AGTACTTTAG CCACTGGCTG 
CTGCTCTGCT CACTGGAGGC TCTCACCATG GAGAAAAAAG GAGGTCGGCG TAATATCTGG 
CTTCAGACTG CTCAACAGAG GGAGAAAATT GGTGGCTGTG CGGGTAACAT GCGGTTAGCT 
GGAGGCGTTA GCATTCTGTC TGATGGTGTT TACAAGCACA GCTTTGAGAG GAAGGAGGGC 
GAGCAGACTC TTACTGGCCT GATAGTGGGT GATAGGATTG TTGTCAGTGA TCAGGACTCT 
GTGCTCATTG GCCTGTCCAC CGGATACGTC ACAGAAATTG CAGTTAACCG AGTTACTTGC 
TCGCTGGACA AAAACCTTTC CAAGTATTCC TCAAACACTG TTTTCCGACT GGACCAGGAT 
GAAGGTGCAG GTGGTCTTAG CACTCATCTC GGAAACCTTT CTATGTTAAT GGAAAACACT 
CCAACAAGTG AAAGACTGAG AGAGTTGATT GTGGAATTCA GGCCTCCTCA GTTCATTGAC 
AGCCTCAGCA GTGTTTTGCC ACGAGATGCC AAGGACATTG TGGCCAACAT TCTGAAAGGA 
CTCAACAAAC CCCAGAAACA AGCAATGAAA AAAGTGCTTC TGTCTAAAGA TTACACGCTT 
ATTGTGGGAA TGCCGGGTAC TGGGAAAACT ACCACCATCT GTACACTGGT GCGAATTCTC 
CATGCTTGTG GCTTCAGTGT TCTTCTGACT AGCTACACCC ACTCTGCTGT GGACAACATC 
CTGCTGAAAC TGAAGAGGTT TAAGATCGGC TTTCTGAGAC TGGGTCGACC TCAAAAAGTC 
CATCATGAGA TCCTGCCTTT CACAGAGGAG GCATGCCGTG CCAAAGGCAT CCAAACTCTA 
GAGGAGCTGG AACAACTGTA CAGCAGAGAG CTGATTGTGG CCACGACTTG TATGGGTGTT 
AAACACCCAA TTTTCAGCCG CAGGCGCTTT GACTTCTGCA TCGTTGATGA GGCGTCTCAG 
ATCAGTCAGC CTGTGTGTAT AGGTCCACTG TTCTACGCCC AGCGCTTTGT CTTGGTGGGA 
GACCATCAAC AGCTTCCTCC TATTGTGCAG AATGCTGAGG CCAGGTCCTT GGGCATGGAT 
GAGAGTTTGT TCAAACGTCT GGAACACCAC AGGGATGCTG TTGTTCAGCT GAATGTGCAG 
TACAGGATGA ACAGTGCGAT CATGTCTCTC AGCAATGCTC TGATGTATGA GGGCCGGCTG 
GAGTGTGGCT CTGAGAAGAC GGCAAACGCA GTGCTTCAGC TGCCCAGTAG AGCTCAGGCA 
GAGGAGGAGC TGGAGCTGTA CGTGTGTCAG CCGCAGTACA GTGCTTGGGT CCAGGCCGCA 
CTGGAGCCCA ACAGTCCTGT CTGTTTCCTG GACACCTCTG AGGTTGCAGC CCCTGAAACT 
GTGGAGAAAA GTGGGATCAG TAATCACACT GAAGCTGTTT TAGTACAAGG CCTTGTTACT 
CTGCTGCTTA AGGCTGGATG CAGAGCATGT GATATTGGGG TCATCGCTCC GTATCGACAA 
CAGCTGAAAG TTATCTCCAG TCTGCTTCAG GGAGACGCCT TTAAAGCACT TGAAGTCAAC 
ACTGTGGACA AATACCAAGG ACGTGACAAG AGCGTCATCA TCGTGTCTTT TGTTAGAAGT 
AATGCTGAAG GCAACCTGGG AGAGTTGCTC CAGGACTGGC GTAGGCTGAA TGTGGCCATC 
ACTAGAGCCA AACACAAGCT CCTCATGCTG GGCTCTGCCC CCACTCTTCG CCGGTACGCA 
CCGCTGGAGA AACTTCTCTG CCACCTTCAG CAGGAAGATA TGATCTTCCA GCTACCTCCT 
GCTGCACACG AGGGACTACC TGGAGTCATC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_687974.6
CDS	299..4414
Translation	MMKCKSSRSSMVSGVQSNISSFFLSKVAVKSPKKCEVGITRSPVSLKTGFVKDENVPCSPNPSAVPETPDSLRIERPSDTSFEKRSPVSRGLHTKGVRGKPSLNARKLFQPQKNSSLESEDTFEFKNGVSHSGSVKRLLHSTENIQSAKKSRTTQDQKITDCGPSSATASQNKFSETLQKAFSKSPEKKIKTESVSLEGSRLKAGPCLKMNEKLNGFSKDSGRSSHHLSFSTSEKENSPTCNVKSEADMGAVSGTNDFDLVMSYSSWIQNGSTEEKGTFLNCPIAKKRPLNTSELQVKEQITDGKTLPVPFEDDFADLMDDWFDEGMEQKSEAPKPKKFSHEKNKGIPEHVVLASGVHNRYWVLDVQDVTSSRSCSEKHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVSGLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGSKQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSARRCDPEAGFLVYLKTGSLHPVVGNHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQLPAIVSDQQTCKYCPQKRNCAIYNRAVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALTMEKKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEIAVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVANILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHEILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQRFVLVGDHQQLPPIVQNAEARSLGMDESLFKRLEHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVAAPETVEKSGISNHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNAEGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTLRRYAPLEKLLCHLQQEDMIFQLPPAAHEGLPGVIF

Target ORF information:

RefSeq Version	XM_682882.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_682882.8

ORF Insert Sequence:

ATGATGAAGT GCAAATCCAG CCGATCATCT ATGGTCAGTG GAGTTCAATC AAACATTTCT 
TCCTTTTTCC TCTCAAAGGT AGCGGTAAAG TCACCTAAAA AATGTGAAGT AGGAATTACA 
AGATCCCCTG TTTCTTTAAA GACTGGGTTT GTGAAGGACG AAAATGTTCC CTGCTCCCCA 
AACCCATCTG CTGTCCCAGA GACGCCTGAC AGTCTGAGAA TCGAGAGGCC TTCAGACACT 
TCATTTGAGA AACGCTCACC TGTTTCACGT GGTCTACATA CTAAAGGAGT CAGGGGTAAA 
CCTAGCCTGA ATGCACGAAA GCTGTTTCAG CCACAGAAAA ACAGCAGTTT AGAGTCAGAG 
GACACTTTTG AGTTCAAGAA TGGCGTAAGT CACTCAGGCT CCGTAAAGAG GTTGTTGCAC 
AGCACTGAAA ACATACAGAG TGCCAAGAAG TCCAGGACTA CTCAAGATCA GAAAATAACT 
GATTGTGGCC CAAGCTCAGC AACGGCCAGT CAAAATAAAT TTTCTGAAAC ATTGCAAAAA 
GCTTTCTCTA AGAGTCCTGA GAAAAAAATC AAAACTGAGT CTGTGAGTTT GGAGGGTAGC 
CGACTGAAGG CTGGACCTTG TTTGAAAATG AATGAAAAAT TAAATGGTTT CTCAAAAGAC 
TCAGGAAGAT CCTCTCATCA TCTGAGCTTT AGTACTAGTG AGAAGGAGAA TTCACCTACT 
TGTAATGTAA AGTCAGAGGC AGACATGGGT GCCGTTTCTG GAACAAATGA CTTTGATCTT 
GTGATGTCGT ATAGTTCTTG GATACAAAAT GGATCTACTG AAGAAAAAGG TACTTTTCTT 
AACTGTCCAA TTGCTAAGAA GAGGCCCTTA AATACATCTG AGCTGCAAGT GAAAGAACAA 
ATTACTGATG GAAAGACTTT GCCTGTACCA TTTGAGGACG ATTTTGCCGA TTTGATGGAC 
GATTGGTTTG ATGAAGGGAT GGAGCAAAAA TCTGAGGCAC CAAAACCCAA GAAGTTCAGT 
CATGAAAAAA ACAAAGGCAT ACCTGAGCAT GTTGTGCTGG CCTCTGGTGT TCATAACCGA 
TATTGGGTGC TGGATGTGCA GGACGTTACC TCGTCAAGAA GCTGCTCAGA GAAGCACTTG 
ACCATCACTT GCTCAAAGAC GAGTCAGGCC ACAGAGACCT GCATTCTCAA AGATGGCTGG 
GAAAGTACTC TGGTAACTGT AGGGGACATT ATTCACTTGG AAGGGAAGTG TGTTTCCGGC 
TTGTGGACAA TTGATAGAGA CTCTGCTTTT CTGATTCTTC TGCCTGACCT GCTGATATCT 
GGGACCAGCA TTGCCAGCAG CATTCGCTGT ATGAGGCGAG GGGTGCTGGG AGAGATGTTC 
AAGGCATTTG ATGGTGGATC CAAGCAGATG TTAAATGGTA CAATAGTTCA TGATATCTTT 
CAAAAAGCAG CCATGTCCTC AGATTTCTCC TCAGAGAAGA TACAGACATT TGCCACGGAG 
GCCTTAAGAA GCCCCAACTA CCTCGGACAA ATGTACAGTC TAAAGCTGAC TCAGGCTGAC 
ATGAGACAGG AAGTTGAAGA ATATCTTCCC TCTATATCTG AATGGGCAAA ACAATACCTC 
CACACTTCCC CACAGGCAGG ACAAAAACAG CTGACTCTAA AACTCCCTAG TGATGGGGCT 
CTGAGCAAAC AAGATGCTTC CAGTAGCGTG ATGGTTACTG ATTTTGTGGA CATTGAGGAG 
AACATTTGGT GTCCACGTTT TGGTCTGAAG GGAAAGATTG ATGTCACAGC TGGAGTTAAG 
ATTCACCGGA GAGGCAGGAA GCCCATGGAA AGAATTGTGC CACTGGAGCT GAAAACTGGC 
AAAGAGTCTA ACTCCATTGA GCATCGCAGT CAGGTTATTC TGTACACACT GATGAGCTCA 
GCTAGACGTT GTGATCCAGA GGCAGGTTTC CTTGTCTACT TGAAGACTGG CAGTCTACAT 
CCTGTTGTGG GCAATCACAT GGACAGGAGA GAGCTGATTA AAATAAGGAA CTCATTGGCC 
CATCACATTG GCAACAACCT GATCAAGGTG GATGGGAAAA GTCAAATGGC CCAATTGCCT 
GCCATTGTAT CTGACCAGCA AACTTGCAAA TATTGTCCTC AGAAAAGGAA CTGTGCAATT 
TACAACAGAG CTGTGGAGAG AGATCCTGTG GAGAAGTGCA GTGAAAATCC ACAGGCCTTT 
GTGCAGTCAG AGAGTGAACA CCTCAGTCAG GTGCACCTCC AGTACTTTAG CCACTGGCTG 
CTGCTCTGCT CACTGGAGGC TCTCACCATG GAGAAAAAAG GAGGTCGGCG TAATATCTGG 
CTTCAGACTG CTCAACAGAG GGAGAAAATT GGTGGCTGTG CGGGTAACAT GCGGTTAGCT 
GGAGGCGTTA GCATTCTGTC TGATGGTGTT TACAAGCACA GCTTTGAGAG GAAGGAGGGC 
GAGCAGACTC TTACTGGCCT GATAGTGGGT GATAGGATTG TTGTCAGTGA TCAGGACTCT 
GTGCTCATTG GCCTGTCCAC CGGATACGTC ACAGAAATTG CAGTTAACCG AGTTACTTGC 
TCGCTGGACA AAAACCTTTC CAAGTATTCC TCAAACACTG TTTTCCGACT GGACCAGGAT 
GAAGGTGCAG GTGGTCTTAG CACTCATCTC GGAAACCTTT CTATGTTAAT GGAAAACACT 
CCAACAAGTG AAAGACTGAG AGAGTTGATT GTGGAATTCA GGCCTCCTCA GTTCATTGAC 
AGCCTCAGCA GTGTTTTGCC ACGAGATGCC AAGGACATTG TGGCCAACAT TCTGAAAGGA 
CTCAACAAAC CCCAGAAACA AGCAATGAAA AAAGTGCTTC TGTCTAAAGA TTACACGCTT 
ATTGTGGGAA TGCCGGGTAC TGGGAAAACT ACCACCATCT GTACACTGGT GCGAATTCTC 
CATGCTTGTG GCTTCAGTGT TCTTCTGACT AGCTACACCC ACTCTGCTGT GGACAACATC 
CTGCTGAAAC TGAAGAGGTT TAAGATCGGC TTTCTGAGAC TGGGTCGACC TCAAAAAGTC 
CATCATGAGA TCCTGCCTTT CACAGAGGAG GCATGCCGTG CCAAAGGCAT CCAAACTCTA 
GAGGAGCTGG AACAACTGTA CAGCAGAGAG CTGATTGTGG CCACGACTTG TATGGGTGTT 
AAACACCCAA TTTTCAGCCG CAGGCGCTTT GACTTCTGCA TCGTTGATGA GGCGTCTCAG 
ATCAGTCAGC CTGTGTGTAT AGGTCCACTG TTCTACGCCC AGCGCTTTGT CTTGGTGGGA 
GACCATCAAC AGCTTCCTCC TATTGTGCAG AATGCTGAGG CCAGGTCCTT GGGCATGGAT 
GAGAGTTTGT TCAAACGTCT GGAACACCAC AGGGATGCTG TTGTTCAGCT GAATGTGCAG 
TACAGGATGA ACAGTGCGAT CATGTCTCTC AGCAATGCTC TGATGTATGA GGGCCGGCTG 
GAGTGTGGCT CTGAGAAGAC GGCAAACGCA GTGCTTCAGC TGCCCAGTAG AGCTCAGGCA 
GAGGAGGAGC TGGAGCTGTA CGTGTGTCAG CCGCAGTACA GTGCTTGGGT CCAGGCCGCA 
CTGGAGCCCA ACAGTCCTGT CTGTTTCCTG GACACCTCTG AGGTTGCAGC CCCTGAAACT 
GTGGAGAAAA GTGGGATCAG TAATCACACT GAAGCTGTTT TAGTACAAGG CCTTGTTACT 
CTGCTGCTTA AGGCTGGATG CAGAGCATGT GATATTGGGG TCATCGCTCC GTATCGACAA 
CAGCTGAAAG TTATCTCCAG TCTGCTTCAG GGAGACGCCT TTAAAGCACT TGAAGTCAAC 
ACTGTGGACA AATACCAAGG ACGTGACAAG AGCGTCATCA TCGTGTCTTT TGTTAGAAGT 
AATGCTGAAG GCAACCTGGG AGAGTTGCTC CAGGACTGGC GTAGGCTGAA TGTGGCCATC 
ACTAGAGCCA AACACAAGCT CCTCATGCTG GGCTCTGCCC CCACTCTTCG CCGGTACGCA 
CCGCTGGAGA AACTTCTCTG CCACCTTCAG CAGGAAGATA TGATCTTCCA GCTACCTCCT 
GCTGCACACG AGGGACTACC TGGAGTCATC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( XM_021480834.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021336509.1 Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

Price & Availability↑

CloneID	ODa18850
Clone ID Related Accession (Same CDS sequence)	XM_021480834.1 , XM_021474283.1 , XM_682882.7
Accession Version	XM_021480834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA 
GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC 
TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT 
TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC 
AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA 
GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG 
TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG 
AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA 
TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG 
GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC 
TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT 
TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC 
TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT 
ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG 
AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT 
TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG 
AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT 
CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG 
AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA 
GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT 
GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG 
CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA 
GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT 
GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT 
GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT 
CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA 
CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT 
GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC 
ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT 
GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG 
AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG 
ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC 
AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC 
TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC 
CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC 
TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA 
CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC 
CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT 
AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG 
CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG 
AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT 
CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA 
GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG 
GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG 
GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG 
TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT 
CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT 
TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG 
CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG 
GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT 
CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC 
CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT 
ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG 
GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC 
TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG 
GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG 
AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG 
GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA 
GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC 
CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC 
CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC 
CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG 
TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT 
GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT 
GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT 
GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC 
CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG 
CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336509.1
CDS	287..4411
Translation	MSTMMKCKSSRSSMVSGVQSNISSFFLSKVAVKSPKKCEVGITRSPVSLKTGFVKDENVPCSPNPSAVPETPDSLRIERPSDTSFEKHSPVSRGLHTKGVRGKPSLKARKLLQPQKSSSLESEDTFEFKNGISHSGSVKRLLHSTENIQSAKKSRTTQDQKITDCGPSSATASQNKYSETLQKAFSKSPEKKIKTESVSLEGSRLKAGPCLKMNEKLNGFSKDSGRSSHHLSFSTSEKENSPTCNVKSEADMGAVSGTNDFDLVMSYSSWIQNGSTEEKGTFLNCPIAKKRPLNTSELQVKEQIADGKTLPVPFEDDFADLMDDWFDEGMEQKSEAPKPKKFSHEKNKGIPEHVVLASGVHNRYWVLDVQDVTSSRSCSEKHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVSGLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGSKQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSARRCDPEAGFLVYLKTGSLHPVVGNHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQLPAIVSDQQTCKYCPQKRNCAIYNRAVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALTMENKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEIAVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVSNILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHEILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQRFVLVGDHQQLPPIVQNAEARSLGMDESLFKRLEHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVPAPETVEKSGISNHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNAEGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTLRRYAPLEKLLCHLQQEDMIFQLPPAAHEGLPGVIF

Target ORF information:

RefSeq Version	XM_021480834.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480834.1

ORF Insert Sequence:

ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA 
GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC 
TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT 
TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC 
AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA 
GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG 
TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG 
AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA 
TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG 
GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC 
TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT 
TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC 
TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT 
ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG 
AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT 
TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG 
AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT 
CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG 
AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA 
GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT 
GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG 
CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA 
GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT 
GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT 
GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT 
CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA 
CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT 
GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC 
ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT 
GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG 
AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG 
ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC 
AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC 
TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC 
CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC 
TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA 
CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC 
CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT 
AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG 
CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG 
AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT 
CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA 
GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG 
GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG 
GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG 
TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT 
CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT 
TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG 
CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG 
GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT 
CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC 
CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT 
ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG 
GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC 
TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG 
GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG 
AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG 
GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA 
GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC 
CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC 
CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC 
CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG 
TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT 
GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT 
GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT 
GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC 
CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG 
CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( XM_021474283.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329958.1 Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

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CloneID	ODa18850
Clone ID Related Accession (Same CDS sequence)	XM_021480834.1 , XM_021474283.1 , XM_682882.7
Accession Version	XM_021474283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA replication ATP-dependent helicase/nuclease DNA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395309.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA 
GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC 
TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT 
TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC 
AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA 
GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG 
TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG 
AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA 
TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG 
GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC 
TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT 
TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC 
TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT 
ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG 
AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT 
TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG 
AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT 
CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG 
AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA 
GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT 
GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG 
CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA 
GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT 
GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT 
GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT 
CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA 
CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT 
GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC 
ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT 
GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG 
AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG 
ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC 
AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC 
TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC 
CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC 
TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA 
CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC 
CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT 
AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG 
CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG 
AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT 
CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA 
GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG 
GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG 
GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG 
TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT 
CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT 
TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG 
CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG 
GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT 
CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC 
CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT 
ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG 
GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC 
TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG 
GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG 
AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG 
GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA 
GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC 
CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC 
CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC 
CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG 
TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT 
GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT 
GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT 
GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC 
CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG 
CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329958.1
CDS	317..4441
Translation	MSTMMKCKSSRSSMVSGVQSNISSFFLSKVAVKSPKKCEVGITRSPVSLKTGFVKDENVPCSPNPSAVPETPDSLRIERPSDTSFEKHSPVSRGLHTKGVRGKPSLKARKLLQPQKSSSLESEDTFEFKNGISHSGSVKRLLHSTENIQSAKKSRTTQDQKITDCGPSSATASQNKYSETLQKAFSKSPEKKIKTESVSLEGSRLKAGPCLKMNEKLNGFSKDSGRSSHHLSFSTSEKENSPTCNVKSEADMGAVSGTNDFDLVMSYSSWIQNGSTEEKGTFLNCPIAKKRPLNTSELQVKEQIADGKTLPVPFEDDFADLMDDWFDEGMEQKSEAPKPKKFSHEKNKGIPEHVVLASGVHNRYWVLDVQDVTSSRSCSEKHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVSGLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGSKQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSARRCDPEAGFLVYLKTGSLHPVVGNHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQLPAIVSDQQTCKYCPQKRNCAIYNRAVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALTMENKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEIAVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVSNILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHEILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQRFVLVGDHQQLPPIVQNAEARSLGMDESLFKRLEHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVPAPETVEKSGISNHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNAEGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTLRRYAPLEKLLCHLQQEDMIFQLPPAAHEGLPGVIF

Target ORF information:

RefSeq Version	XM_021474283.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474283.1

ORF Insert Sequence:

ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA 
GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC 
TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT 
TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC 
AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA 
GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG 
TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG 
AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA 
TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG 
GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC 
TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT 
TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC 
TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT 
ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG 
AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT 
TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG 
AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT 
CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG 
AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA 
GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT 
GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG 
CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA 
GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT 
GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT 
GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT 
CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA 
CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT 
GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC 
ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT 
GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG 
AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG 
ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC 
AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC 
TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC 
CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC 
TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA 
CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC 
CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT 
AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG 
CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG 
AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT 
CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA 
GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG 
GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG 
GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG 
TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT 
CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT 
TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG 
CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG 
GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT 
CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC 
CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT 
ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG 
GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC 
TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG 
GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG 
AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG 
GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA 
GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC 
CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC 
CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC 
CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG 
TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT 
GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT 
GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT 
GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC 
CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG 
CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNA2 ( XM_682882.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_687974.5 Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

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CloneID	ODa18850
Clone ID Related Accession (Same CDS sequence)	XM_021480834.1 , XM_021474283.1 , XM_682882.7
Accession Version	XM_682882.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA replication ATP-dependent helicase/nuclease DNA2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682882.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA 
GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC 
TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT 
TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC 
AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA 
GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG 
TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG 
AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA 
TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG 
GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC 
TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT 
TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC 
TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT 
ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG 
AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT 
TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG 
AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT 
CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG 
AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA 
GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT 
GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG 
CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA 
GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT 
GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT 
GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT 
CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA 
CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT 
GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC 
ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT 
GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG 
AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG 
ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC 
AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC 
TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC 
CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC 
TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA 
CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC 
CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT 
AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG 
CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG 
AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT 
CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA 
GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG 
GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG 
GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG 
TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT 
CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT 
TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG 
CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG 
GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT 
CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC 
CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT 
ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG 
GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC 
TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG 
GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG 
AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG 
GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA 
GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC 
CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC 
CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC 
CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG 
TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT 
GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT 
GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT 
GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC 
CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG 
CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_687974.5
CDS	304..4428
Translation	MSTMMKCKSSRSSMVSGVQSNISSFFLSKVAVKSPKKCEVGITRSPVSLKTGFVKDENVPCSPNPSAVPETPDSLRIERPSDTSFEKHSPVSRGLHTKGVRGKPSLKARKLLQPQKSSSLESEDTFEFKNGISHSGSVKRLLHSTENIQSAKKSRTTQDQKITDCGPSSATASQNKYSETLQKAFSKSPEKKIKTESVSLEGSRLKAGPCLKMNEKLNGFSKDSGRSSHHLSFSTSEKENSPTCNVKSEADMGAVSGTNDFDLVMSYSSWIQNGSTEEKGTFLNCPIAKKRPLNTSELQVKEQIADGKTLPVPFEDDFADLMDDWFDEGMEQKSEAPKPKKFSHEKNKGIPEHVVLASGVHNRYWVLDVQDVTSSRSCSEKHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVSGLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGSKQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSARRCDPEAGFLVYLKTGSLHPVVGNHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQLPAIVSDQQTCKYCPQKRNCAIYNRAVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALTMENKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEIAVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVSNILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHEILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQRFVLVGDHQQLPPIVQNAEARSLGMDESLFKRLEHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVPAPETVEKSGISNHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNAEGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTLRRYAPLEKLLCHLQQEDMIFQLPPAAHEGLPGVIF

Target ORF information:

RefSeq Version	XM_682882.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DNA replication helicase/nuclease 2 (dna2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_682882.7

ORF Insert Sequence:

ATGTCTACAA TGATGAAGTG CAAATCCAGC CGATCATCTA TGGTCAGTGG AGTTCAATCA 
AACATTTCTT CATTTTTCCT CTCAAAGGTA GCGGTAAAGT CACCTAAAAA ATGTGAAGTA 
GGAATTACAA GATCCCCTGT TTCTTTAAAG ACTGGGTTTG TGAAGGACGA AAATGTTCCC 
TGCTCCCCAA ACCCATCTGC TGTCCCAGAG ACGCCTGACA GTCTGAGAAT CGAGAGGCCT 
TCAGACACTT CATTTGAGAA GCACTCACCT GTTTCACGTG GTCTACATAC TAAAGGAGTC 
AGGGGTAAAC CTAGCCTGAA GGCACGAAAG CTGTTGCAGC CACAGAAAAG CAGCAGTTTA 
GAGTCAGAGG ACACTTTTGA GTTCAAGAAT GGCATAAGTC ACTCAGGCTC CGTAAAGAGG 
TTGTTGCACA GCACTGAAAA CATACAGAGT GCCAAGAAGT CCAGGACTAC TCAAGATCAG 
AAAATAACTG ATTGTGGCCC AAGCTCAGCA ACGGCCAGTC AAAATAAATA TTCTGAAACA 
TTGCAAAAAG CTTTCTCTAA GAGTCCTGAG AAAAAAATCA AAACTGAGTC TGTGAGTTTG 
GAGGGTAGCC GACTGAAGGC TGGACCTTGT TTGAAAATGA ATGAAAAATT AAATGGTTTC 
TCAAAAGACT CAGGAAGATC CTCTCATCAT CTGAGCTTTA GTACTAGTGA GAAGGAGAAT 
TCACCTACTT GTAATGTGAA GTCAGAGGCA GACATGGGTG CCGTTTCTGG AACAAATGAC 
TTTGATCTTG TGATGTCGTA TAGTTCTTGG ATACAAAATG GATCTACTGA AGAAAAAGGT 
ACTTTTCTTA ACTGTCCAAT TGCTAAGAAG AGGCCCTTAA ATACATCTGA GCTGCAAGTG 
AAAGAACAAA TTGCTGATGG AAAGACTTTG CCTGTACCAT TTGAGGACGA TTTTGCCGAT 
TTGATGGACG ATTGGTTTGA TGAAGGGATG GAGCAAAAAT CTGAGGCACC AAAACCCAAG 
AAGTTCAGTC ATGAAAAAAA CAAAGGCATA CCTGAGCATG TTGTGCTGGC CTCTGGTGTT 
CATAACCGAT ATTGGGTGCT GGATGTGCAG GACGTTACCT CGTCAAGAAG CTGCTCAGAG 
AAGCACTTGA CCATCACTTG CTCAAAGACG AGTCAGGCCA CAGAGACCTG CATTCTCAAA 
GATGGCTGGG AAAGTACTCT GGTAACTGTA GGGGACATTA TTCACTTGGA AGGGAAGTGT 
GTTTCCGGCT TGTGGACAAT TGATAGAGAC TCTGCTTTCC TGATTCTTCT GCCTGACCTG 
CTGATATCTG GGACCAGCAT TGCCAGCAGC ATTCGCTGTA TGAGGCGAGG GGTGCTGGGA 
GAGATGTTCA AGGCATTTGA TGGTGGATCC AAGCAGATGT TAAATGGTAC AATAGTTCAT 
GATATCTTTC AAAAAGCAGC CATGTCCTCA GATTTCTCCT CAGAGAAGAT ACAGACATTT 
GCCACGGAGG CCTTAAGAAG CCCCAACTAC CTCGGACAAA TGTACAGTCT AAAGCTGACT 
CAGGCTGACA TGAGACAGGA AGTTGAAGAA TATCTTCCCT CTATATCTGA ATGGGCAAAA 
CAATACCTCC ACACTTCCCC ACAGGCAGGA CAAAAACAGC TGACTCTAAA ACTCCCTAGT 
GATGGGGCTC TGAGCAAACA AGATGCTTCC AGTAGCGTGA TGGTTACTGA TTTTGTGGAC 
ATTGAGGAGA ACATTTGGTG TCCACGTTTT GGTCTGAAGG GAAAGATTGA TGTCACAGCT 
GGAGTTAAGA TTCACCGGAG AGGCAGGAAG CCCATGGAAA GAATTGTGCC ACTGGAGCTG 
AAAACTGGCA AAGAGTCTAA CTCCATTGAG CATCGCAGTC AGGTTATTCT GTACACACTG 
ATGAGCTCAG CTAGACGTTG TGATCCAGAG GCAGGTTTCC TTGTCTACTT GAAGACTGGC 
AGTCTACATC CTGTTGTGGG CAATCACATG GACAGGAGAG AGCTGATTAA AATAAGGAAC 
TCATTGGCCC ATCACATTGG CAACAACCTG ATCAAGGTGG ATGGGAAAAG TCAAATGGCC 
CAATTGCCTG CCATTGTATC TGACCAGCAA ACTTGCAAAT ATTGTCCTCA GAAAAGGAAC 
TGTGCAATTT ACAACAGAGC TGTGGAGAGA GATCCTGTGG AGAAGTGCAG TGAAAATCCA 
CAGGCCTTTG TGCAGTCAGA GAGTGAACAC CTCAGTCAGG TGCACCTCCA GTACTTTAGC 
CACTGGCTGC TGCTCTGCTC ACTGGAGGCT CTCACCATGG AGAATAAAGG AGGTCGGCGT 
AATATCTGGC TTCAGACTGC TCAACAGAGG GAGAAAATTG GTGGCTGTGC GGGTAACATG 
CGGTTAGCTG GAGGCGTTAG CATTCTGTCT GATGGTGTTT ACAAGCACAG CTTTGAGAGG 
AAGGAGGGCG AGCAGACTCT TACTGGCCTG ATAGTGGGTG ATAGGATTGT TGTCAGTGAT 
CAGGACTCTG TGCTCATTGG CCTGTCCACC GGATACGTCA CAGAAATTGC AGTTAACCGA 
GTTACTTGCT CGCTGGACAA AAACCTTTCC AAGTATTCCT CAAACACTGT TTTCCGACTG 
GACCAGGATG AAGGTGCAGG TGGTCTTAGC ACTCATCTCG GAAACCTTTC TATGTTAATG 
GAAAACACTC CAACAAGTGA AAGACTGAGA GAGTTGATTG TGGAATTCAG GCCTCCTCAG 
TTCATTGACA GCCTCAGCAG TGTTTTGCCA CGAGATGCCA AGGACATTGT GTCCAACATT 
CTGAAAGGAC TCAACAAACC CCAGAAACAA GCAATGAAAA AAGTGCTTCT GTCTAAAGAT 
TACACGCTTA TTGTGGGAAT GCCGGGTACT GGGAAAACTA CCACCATCTG TACACTGGTG 
CGAATTCTCC ATGCTTGTGG CTTCAGTGTT CTTCTGACTA GCTACACCCA CTCTGCTGTG 
GACAACATCC TGCTGAAACT GAAGAGGTTT AAGATCGGCT TTCTGAGACT GGGTCGACCT 
CAAAAAGTCC ATCATGAGAT CCTGCCTTTC ACAGAGGAGG CATGCCGTGC CAAAGGCATC 
CAAACTCTAG AGGAGCTGGA ACAACTGTAC AGCAGAGAGC TGATTGTGGC CACGACTTGT 
ATGGGTGTTA AACACCCAAT TTTCAGCCGC AGGCGCTTTG ACTTCTGCAT CGTTGATGAG 
GCGTCTCAGA TCAGTCAGCC TGTGTGTATA GGTCCACTGT TCTACGCCCA GCGCTTTGTC 
TTGGTGGGAG ACCATCAACA GCTTCCTCCT ATTGTGCAGA ATGCTGAGGC CAGGTCCTTG 
GGCATGGATG AGAGTTTGTT CAAACGTCTG GAACACCACA GGGATGCTGT TGTTCAGCTG 
AATGTGCAGT ACAGGATGAA CAGTGCGATC ATGTCTCTCA GCAATGCTCT GATGTATGAG 
GGCCGGCTGG AGTGTGGCTC TGAGAAGACG GCAAACGCAG TGCTTCAGCT GCCCAGTAGA 
GCTCAGGCAG AGGAGGAGCT GGAGCTGTAC GTGTGTCAGC CGCAGTACAG TGCTTGGGTC 
CAGGCCGCAC TGGAGCCCAA CAGTCCTGTC TGTTTCCTGG ACACCTCTGA GGTTCCAGCC 
CCTGAAACTG TGGAGAAAAG TGGGATCAGT AATCACACTG AAGCTGTTTT AGTACAAGGC 
CTTGTTACTC TGCTGCTTAA GGCTGGATGC AGAGCATGTG ATATTGGGGT CATCGCTCCG 
TATCGACAAC AGCTGAAAGT TATCTCCAGT CTGCTTCAGG GAGACGCCTT TAAAGCACTT 
GAAGTCAACA CTGTGGACAA ATACCAAGGA CGTGACAAGA GCGTCATCAT CGTGTCTTTT 
GTTAGAAGTA ATGCTGAAGG CAACCTGGGA GAGTTGCTCC AGGACTGGCG TAGGCTGAAT 
GTGGCCATCA CTAGAGCCAA ACACAAGCTC CTCATGCTGG GCTCTGCCCC CACTCTTCGC 
CGGTACGCAC CGCTGGAGAA ACTTCTCTGC CACCTTCAGC AGGAAGATAT GATCTTCCAG 
CTACCTCCTG CTGCACACGA GGGACTACCT GGAGTCATCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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DNA2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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