ulk1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ulk1b
Entrez Gene ID	558848
Full Name	unc-51 like autophagy activating kinase 1
Synonyms	si:dkey-11o18.7,ulk1
General protein information	Preferred Names unc-51 like autophagy activating kinase 1 Names serine/threonine-protein kinase ULK2 serine/threonine-protein kinase ULK1 unc-51 like autophagy activating kinase 1b unc-51-like kinase 1 unc-51-like kinase 1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002665925.5	XP_002665971.2	serine/threonine-protein kinase ULK1 isoform X1
XM_005161121.4	XP_005161178.1	serine/threonine-protein kinase ULK1 isoform X2
XM_021469039.1	XP_021324714.1	serine/threonine-protein kinase ULK1 isoform X3
XM_005161121.3	XP_005161178.1	serine/threonine-protein kinase ULK1 isoform X2
XM_002665925.6	XP_002665971.2	serine/threonine-protein kinase ULK1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ULK1	XP_415091.2
Xenopus tropicalis (tropical clawed frog)	ulk1	NP_001106388.1
Homo sapiens (human)	ULK1	NP_003556.1
Pan troglodytes (chimpanzee)	ULK1	XP_001148902.3
Macaca mulatta (Rhesus monkey)	LOC709746	XP_001105326.2
Bos taurus (cattle)	ULK1	NP_001192856.1
Danio rerio (zebrafish)	ulk1b	XP_002665971.2
Rattus norvegicus (Norway rat)	Ulk1	NP_001101811.1
Canis lupus familiaris (dog)	ULK1	XP_534635.3
Mus musculus (house mouse)	Ulk1	NP_033495.2

Gene Ontology

The following ulk1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ulk1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151035	XM_021469039.1 Latest version!	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa25707	XM_002665925.6 Latest version!	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa25708	XM_005161121.4 Latest version!	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa25708	XM_005161121.3	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa25707	XM_002665925.5	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ulk1b ( XM_021469039.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324714.1 Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa151035
Clone ID Related Accession (Same CDS sequence)	XM_021469039.1
Accession Version	XM_021469039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase ULK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGCAG GCCGAGTGAG 
TTTTCTGGGA GTAGCTATGG CAACTATGGG CAGTCTGTAC CCATTCCAGT GCCCACACAG 
ATTCACAACT ACCAGCGCAT GGAGCAAAAC TTCCAGTTTC CAGGTCAAGA GGGTTCTCCT 
CGATCTGGTT TGGCTCAGAG ATGCAGTAGT GGTAGTTTTC TGGCCTGTGG AAGGACAGGG 
CCTTCTCCTC CTCGGTCTGG TTCTGCTGTC ATGTCCCGCA GGCTTTCCAC AGGAGGCAGC 
AAACCCTTCC AGCTCTCTCC ACAGGTGGGC ACCATTCCTG AATTGCCAGG CCAAGTTTGC 
CCAGCGAGTG CAGAAGTGGG TCACAGGGGC TCTCGTGGTG GAGAAGCCAA GACTTGGCCT 
CAGCAGCAGG GTCTAGGCAC GCGGCTCAAC AGTGCCCCCT GTTTGCTGGA GGCCGCTGGA 
GGCTGCAGGC AGAAGATCAG GAAGCAGCAC TCAGATCCAG TGGTCGCCCC TCAGGCAAAC 
GTGATGCCCT TCAGGACCCT GCACTCCTCA CCCAGGCTCA GTGAACTTAT GCAACGCAAC 
CCCCTACCAA CTATCCTGGG TTCTCCTTCA AGAGCCATGC CCCCGTTTGA GTTTCCCAAG 
CCTCCGAGTT CCCCAAACAT GGTCACATTT CTGACCCAGC AGGGCCTCAG AACAGCTCAG 
GGAGAGCCTA GTTATTCACC TGCCTACCAG CCAGAGGAGC GGAAAGGATT TGGGAGGTCT 
CAGAGCGCTG GTCGCCTGTC TGATGTGCTG CTGATGGCAG CATTTGGAGG ACAGAGAGGA 
GAACGAGGCA GCATGGAGAA TCTCAACACT GACAGAGCCA TCGACATCAC AGCGCCACCT 
GGTGGTGGGG GCATCGTGGT CGGCTCAGGT AGCCCTGCTC GAGTGGTGTT CACCATTGGT 
TCTCCACCCA GTGGAGCAAC ACCTCCGCAG AATTCTCGCT CAAGAAAGCT CTCCGGTTCT 
TCAAGCTCCG TTAGTCCAGT AGGCTCTCTC ACCAGCCGCT ACACACAAGC AGGCAATTAC 
ATGGACTGCT ATGAGGGATC TTCCAGTCCC CGCTACGGTT TCTCTGACCC CATCTCAGCC 
GTGACTTTTG AAGCCCCTGA ACTACCAGAG GAGACCCTGA TGGAACAGGA ACACACGGAG 
ACTCTTTGCA GGCTCCGCTT CATGCTGGAC TTTGCTCGTT GTATGGTGGA GGTAGCAGGA 
GCTCGGAGTG GAGAGATGGC CCAGACTGAG CTCTCCTCCA CCAGCCTGCT CCAACAACAG 
AGCCTGATGG CCGACCAGAT CAGCTCTCTG AGCCGAGAGT GGAGTTATGC TGAGCAGTTG 
GTGCTTTACA TGAAGACCGC AGAGCTTCTG TCATCTTCAC TACAAACAGC CATGGAAGGA 
ATCAAGCAGG GAAAATTGTA CCCCTCAAGC ACCGTCAAAC AAGTGGTCAG GAGGTTGAAT 
GACCTGTACA AGTCCAGCGT GATGTCCTGT CGGTCTCTGA GCAATCAGCT GGAGCGATTC 
TTCACCCGAA AACACAAACT CATGGACCAC ATCAACAGCA TCACTGCAGA AAGGCTCCTC 
TTCGGCCACA CTGTGCAAAT GGTGCAGGCT GCAGCTCTGG ATGAGATGTT TCATCAGGGG 
GAAGCATCAA TTCAGCGCTA CCATAAGGCC TTGCTGCTCA TGGAGGGTCT GTCGCTTCTC 
CTCACTGAAC AGGCTGACAT CCTTAGCATT AGCAAGTGTA AACAATGCAT TGAACGCCGT 
CTCACCGCCT TGCAGTCGGG TCTCTGTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324714.1
CDS	550..3582
Translation	METVGKFEFSRKDLIGHGAFAVVFKGRHREKHEWEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALHDFQETASSVYLVMEYCNGGDLADYLHSKGTLSEDTIRVFLQQITGAMRVLQAKGIIHRDLKPQNILLSHPAGRKSHFNNTCIKIADFGFARYLQNNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVFQCLTGKAPFQASSPQDLRLFYEKNKTLSPNIPRETSTHLRHLLLGLLQRNHKDRMDFDEFFRHPFLEASSSMKKSTPVTVTCFPNSASASSCSSSSTSHLASPPQSLAEIQHVRAKALASPTQDSPGYLLKDSGGGGGGSSSKNSSCDTDDFVMVPAHFPSELTCDMPTGKVLQDSLMYSGSSLLASGGQCSQGKTPPRSPSFSSSPGPSGRPSEFSGSSYGNYGQSVPIPVPTQIHNYQRMEQNFQFPGQEGSPRSGLAQRCSSGSFLACGRTGPSPPRSGSAVMSRRLSTGGSKPFQLSPQVGTIPELPGQVCPASAEVGHRGSRGGEAKTWPQQQGLGTRLNSAPCLLEAAGGCRQKIRKQHSDPVVAPQANVMPFRTLHSSPRLSELMQRNPLPTILGSPSRAMPPFEFPKPPSSPNMVTFLTQQGLRTAQGEPSYSPAYQPEERKGFGRSQSAGRLSDVLLMAAFGGQRGERGSMENLNTDRAIDITAPPGGGGIVVGSGSPARVVFTIGSPPSGATPPQNSRSRKLSGSSSSVSPVGSLTSRYTQAGNYMDCYEGSSSPRYGFSDPISAVTFEAPELPEETLMEQEHTETLCRLRFMLDFARCMVEVAGARSGEMAQTELSSTSLLQQQSLMADQISSLSREWSYAEQLVLYMKTAELLSSSLQTAMEGIKQGKLYPSSTVKQVVRRLNDLYKSSVMSCRSLSNQLERFFTRKHKLMDHINSITAERLLFGHTVQMVQAAALDEMFHQGEASIQRYHKALLLMEGLSLLLTEQADILSISKCKQCIERRLTALQSGLCV

Target ORF information:

RefSeq Version	XM_021469039.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469039.1

ORF Insert Sequence:

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGCAG GCCGAGTGAG 
TTTTCTGGGA GTAGCTATGG CAACTATGGG CAGTCTGTAC CCATTCCAGT GCCCACACAG 
ATTCACAACT ACCAGCGCAT GGAGCAAAAC TTCCAGTTTC CAGGTCAAGA GGGTTCTCCT 
CGATCTGGTT TGGCTCAGAG ATGCAGTAGT GGTAGTTTTC TGGCCTGTGG AAGGACAGGG 
CCTTCTCCTC CTCGGTCTGG TTCTGCTGTC ATGTCCCGCA GGCTTTCCAC AGGAGGCAGC 
AAACCCTTCC AGCTCTCTCC ACAGGTGGGC ACCATTCCTG AATTGCCAGG CCAAGTTTGC 
CCAGCGAGTG CAGAAGTGGG TCACAGGGGC TCTCGTGGTG GAGAAGCCAA GACTTGGCCT 
CAGCAGCAGG GTCTAGGCAC GCGGCTCAAC AGTGCCCCCT GTTTGCTGGA GGCCGCTGGA 
GGCTGCAGGC AGAAGATCAG GAAGCAGCAC TCAGATCCAG TGGTCGCCCC TCAGGCAAAC 
GTGATGCCCT TCAGGACCCT GCACTCCTCA CCCAGGCTCA GTGAACTTAT GCAACGCAAC 
CCCCTACCAA CTATCCTGGG TTCTCCTTCA AGAGCCATGC CCCCGTTTGA GTTTCCCAAG 
CCTCCGAGTT CCCCAAACAT GGTCACATTT CTGACCCAGC AGGGCCTCAG AACAGCTCAG 
GGAGAGCCTA GTTATTCACC TGCCTACCAG CCAGAGGAGC GGAAAGGATT TGGGAGGTCT 
CAGAGCGCTG GTCGCCTGTC TGATGTGCTG CTGATGGCAG CATTTGGAGG ACAGAGAGGA 
GAACGAGGCA GCATGGAGAA TCTCAACACT GACAGAGCCA TCGACATCAC AGCGCCACCT 
GGTGGTGGGG GCATCGTGGT CGGCTCAGGT AGCCCTGCTC GAGTGGTGTT CACCATTGGT 
TCTCCACCCA GTGGAGCAAC ACCTCCGCAG AATTCTCGCT CAAGAAAGCT CTCCGGTTCT 
TCAAGCTCCG TTAGTCCAGT AGGCTCTCTC ACCAGCCGCT ACACACAAGC AGGCAATTAC 
ATGGACTGCT ATGAGGGATC TTCCAGTCCC CGCTACGGTT TCTCTGACCC CATCTCAGCC 
GTGACTTTTG AAGCCCCTGA ACTACCAGAG GAGACCCTGA TGGAACAGGA ACACACGGAG 
ACTCTTTGCA GGCTCCGCTT CATGCTGGAC TTTGCTCGTT GTATGGTGGA GGTAGCAGGA 
GCTCGGAGTG GAGAGATGGC CCAGACTGAG CTCTCCTCCA CCAGCCTGCT CCAACAACAG 
AGCCTGATGG CCGACCAGAT CAGCTCTCTG AGCCGAGAGT GGAGTTATGC TGAGCAGTTG 
GTGCTTTACA TGAAGACCGC AGAGCTTCTG TCATCTTCAC TACAAACAGC CATGGAAGGA 
ATCAAGCAGG GAAAATTGTA CCCCTCAAGC ACCGTCAAAC AAGTGGTCAG GAGGTTGAAT 
GACCTGTACA AGTCCAGCGT GATGTCCTGT CGGTCTCTGA GCAATCAGCT GGAGCGATTC 
TTCACCCGAA AACACAAACT CATGGACCAC ATCAACAGCA TCACTGCAGA AAGGCTCCTC 
TTCGGCCACA CTGTGCAAAT GGTGCAGGCT GCAGCTCTGG ATGAGATGTT TCATCAGGGG 
GAAGCATCAA TTCAGCGCTA CCATAAGGCC TTGCTGCTCA TGGAGGGTCT GTCGCTTCTC 
CTCACTGAAC AGGCTGACAT CCTTAGCATT AGCAAGTGTA AACAATGCAT TGAACGCCGT 
CTCACCGCCT TGCAGTCGGG TCTCTGTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ulk1b ( XM_002665925.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002665971.2 Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25707
Clone ID Related Accession (Same CDS sequence)	XM_002665925.5 , XM_002665925.6
Accession Version	XM_002665925.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase ULK1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002665925.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGCAG GCCGAGTGAG 
TTTTCTGGGA GTAGCTATGG CAACTATGGG CAGTCTGTAC CCATTCCAGT GCCCACACAG 
ATTCACAACT ACCAGCGCAT GGAGCAAAAC TTCCAGTTTC CAGGTCAAGA GGGTTCTCCT 
CGATCTGGTT TGGCTCAGAG ATGCAGTAGT GGTAGTTTTC TGGCCTGTGG AAGGACAGGG 
CCTTCTCCTC CTCGGTCTGG TTCTGCTGTC ATGTCCCGCA GGCTTTCCAC AGGAGGCAGC 
AAACCCTTCC AGCTCTCTCC ACAGGTGGGC ACCATTCCTG AATTGCCAGG CCAAGTTTGC 
CCAGCGAGTG CAGAAGTGGG TCACAGGGGC TCTCGTGGTG GAGAAGCCAA GACTTGGCCT 
CAGCAGCAGG GTCTAGGCAC GCGGCTCAAC AGTGCCCCCT GTTTGCTGGA GGCCGCTGGA 
GGCTGCAGGC AGAAGATCAG GAAGCAGCAC TCAGATCCAG TGGTCGCCCC TCAGGCAAAC 
GTGATGCCCT TCAGGACCCT GCACTCCTCA CCCAGGCTCA GTGAACTTAT GCAACGCAAC 
CCCCTACCAA CTATCCTGGG TTCTCCTTCA AGAGCCATGC CCCCGTTTGA GTTTCCCAAG 
CCTCCGAGTT CCCCAAACAT GGTCACATTT CTGACCCAGC AGGGCCTCAG AACAGCTCAG 
GGAGAGCCTA GTTATTCACC TGCCTACCAG CCAGAGGAGC GGAAAGGATT TGGGAGGTCT 
CAGAGCGCTG GTCGCCTGTC TGATGTGCTG CTGATGGCAG CATTTGGAGG ACAGAGAGGA 
GAACGAGGCA GCATGGAGAA TCTCAACACT GACAGAGCCA TCGACATCAC AGCGCCACCT 
GGTGGTGGGG GCATCGTGGT CGGCTCAGGT AGCCCTGCTC GAGTGGTGTT CACCATTGGT 
TCTCCACCCA GTGGAGCAAC ACCTCCGCAG AATTCTCGCT CAAGAAAGCT CTCCGCAGGT 
TCTTCAAGCT CCGTTAGTCC AGTAGGCTCT CTCACCAGCC GCTACACACA AGCAGGCAAT 
TACATGGACT GCTATGAGGG ATCTTCCAGT CCCCGCTACG GTTTCTCTGA CCCCATCTCA 
GCCGTGACTT TTGAAGCCCC TGAACTACCA GAGGAGACCC TGATGGAACA GGAACACACG 
GAGACTCTTT GCAGGCTCCG CTTCATGCTG GACTTTGCTC GTTGTATGGT GGAGGTAGCA 
GGAGCTCGGA GTGGAGAGAT GGCCCAGACT GAGCTCTCCT CCACCAGCCT GCTCCAACAA 
CAGAGCCTGA TGGCCGACCA GATCAGCTCT CTGAGCCGAG AGTGGAGTTA TGCTGAGCAG 
TTGGTGCTTT ACATGAAGAC CGCAGAGCTT CTGTCATCTT CACTACAAAC AGCCATGGAA 
GGAATCAAGC AGGGAAAATT GTACCCCTCA AGCACCGTCA AACAAGTGGT CAGGAGGTTG 
AATGACCTGT ACAAGTCCAG CGTGATGTCC TGTCGGTCTC TGAGCAATCA GCTGGAGCGA 
TTCTTCACCC GAAAACACAA ACTCATGGAC CACATCAACA GCATCACTGC AGAAAGGCTC 
CTCTTCGGCC ACACTGTGCA AATGGTGCAG GCTGCAGCTC TGGATGAGAT GTTTCATCAG 
GGGGAAGCAT CAATTCAGCG CTACCATAAG GCCTTGCTGC TCATGGAGGG TCTGTCGCTT 
CTCCTCACTG AACAGGCTGA CATCCTTAGC ATTAGCAAGT GTAAACAATG CATTGAACGC 
CGTCTCACCG CCTTGCAGTC GGGTCTCTGT GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665971.2
CDS	531..3566
Translation	METVGKFEFSRKDLIGHGAFAVVFKGRHREKHEWEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALHDFQETASSVYLVMEYCNGGDLADYLHSKGTLSEDTIRVFLQQITGAMRVLQAKGIIHRDLKPQNILLSHPAGRKSHFNNTCIKIADFGFARYLQNNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVFQCLTGKAPFQASSPQDLRLFYEKNKTLSPNIPRETSTHLRHLLLGLLQRNHKDRMDFDEFFRHPFLEASSSMKKSTPVTVTCFPNSASASSCSSSSTSHLASPPQSLAEIQHVRAKALASPTQDSPGYLLKDSGGGGGGSSSKNSSCDTDDFVMVPAHFPSELTCDMPTGKVLQDSLMYSGSSLLASGGQCSQGKTPPRSPSFSSSPGPSGRPSEFSGSSYGNYGQSVPIPVPTQIHNYQRMEQNFQFPGQEGSPRSGLAQRCSSGSFLACGRTGPSPPRSGSAVMSRRLSTGGSKPFQLSPQVGTIPELPGQVCPASAEVGHRGSRGGEAKTWPQQQGLGTRLNSAPCLLEAAGGCRQKIRKQHSDPVVAPQANVMPFRTLHSSPRLSELMQRNPLPTILGSPSRAMPPFEFPKPPSSPNMVTFLTQQGLRTAQGEPSYSPAYQPEERKGFGRSQSAGRLSDVLLMAAFGGQRGERGSMENLNTDRAIDITAPPGGGGIVVGSGSPARVVFTIGSPPSGATPPQNSRSRKLSAGSSSSVSPVGSLTSRYTQAGNYMDCYEGSSSPRYGFSDPISAVTFEAPELPEETLMEQEHTETLCRLRFMLDFARCMVEVAGARSGEMAQTELSSTSLLQQQSLMADQISSLSREWSYAEQLVLYMKTAELLSSSLQTAMEGIKQGKLYPSSTVKQVVRRLNDLYKSSVMSCRSLSNQLERFFTRKHKLMDHINSITAERLLFGHTVQMVQAAALDEMFHQGEASIQRYHKALLLMEGLSLLLTEQADILSISKCKQCIERRLTALQSGLCV

Target ORF information:

RefSeq Version	XM_002665925.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665925.5

ORF Insert Sequence:

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGCAG GCCGAGTGAG 
TTTTCTGGGA GTAGCTATGG CAACTATGGG CAGTCTGTAC CCATTCCAGT GCCCACACAG 
ATTCACAACT ACCAGCGCAT GGAGCAAAAC TTCCAGTTTC CAGGTCAAGA GGGTTCTCCT 
CGATCTGGTT TGGCTCAGAG ATGCAGTAGT GGTAGTTTTC TGGCCTGTGG AAGGACAGGG 
CCTTCTCCTC CTCGGTCTGG TTCTGCTGTC ATGTCCCGCA GGCTTTCCAC AGGAGGCAGC 
AAACCCTTCC AGCTCTCTCC ACAGGTGGGC ACCATTCCTG AATTGCCAGG CCAAGTTTGC 
CCAGCGAGTG CAGAAGTGGG TCACAGGGGC TCTCGTGGTG GAGAAGCCAA GACTTGGCCT 
CAGCAGCAGG GTCTAGGCAC GCGGCTCAAC AGTGCCCCCT GTTTGCTGGA GGCCGCTGGA 
GGCTGCAGGC AGAAGATCAG GAAGCAGCAC TCAGATCCAG TGGTCGCCCC TCAGGCAAAC 
GTGATGCCCT TCAGGACCCT GCACTCCTCA CCCAGGCTCA GTGAACTTAT GCAACGCAAC 
CCCCTACCAA CTATCCTGGG TTCTCCTTCA AGAGCCATGC CCCCGTTTGA GTTTCCCAAG 
CCTCCGAGTT CCCCAAACAT GGTCACATTT CTGACCCAGC AGGGCCTCAG AACAGCTCAG 
GGAGAGCCTA GTTATTCACC TGCCTACCAG CCAGAGGAGC GGAAAGGATT TGGGAGGTCT 
CAGAGCGCTG GTCGCCTGTC TGATGTGCTG CTGATGGCAG CATTTGGAGG ACAGAGAGGA 
GAACGAGGCA GCATGGAGAA TCTCAACACT GACAGAGCCA TCGACATCAC AGCGCCACCT 
GGTGGTGGGG GCATCGTGGT CGGCTCAGGT AGCCCTGCTC GAGTGGTGTT CACCATTGGT 
TCTCCACCCA GTGGAGCAAC ACCTCCGCAG AATTCTCGCT CAAGAAAGCT CTCCGCAGGT 
TCTTCAAGCT CCGTTAGTCC AGTAGGCTCT CTCACCAGCC GCTACACACA AGCAGGCAAT 
TACATGGACT GCTATGAGGG ATCTTCCAGT CCCCGCTACG GTTTCTCTGA CCCCATCTCA 
GCCGTGACTT TTGAAGCCCC TGAACTACCA GAGGAGACCC TGATGGAACA GGAACACACG 
GAGACTCTTT GCAGGCTCCG CTTCATGCTG GACTTTGCTC GTTGTATGGT GGAGGTAGCA 
GGAGCTCGGA GTGGAGAGAT GGCCCAGACT GAGCTCTCCT CCACCAGCCT GCTCCAACAA 
CAGAGCCTGA TGGCCGACCA GATCAGCTCT CTGAGCCGAG AGTGGAGTTA TGCTGAGCAG 
TTGGTGCTTT ACATGAAGAC CGCAGAGCTT CTGTCATCTT CACTACAAAC AGCCATGGAA 
GGAATCAAGC AGGGAAAATT GTACCCCTCA AGCACCGTCA AACAAGTGGT CAGGAGGTTG 
AATGACCTGT ACAAGTCCAG CGTGATGTCC TGTCGGTCTC TGAGCAATCA GCTGGAGCGA 
TTCTTCACCC GAAAACACAA ACTCATGGAC CACATCAACA GCATCACTGC AGAAAGGCTC 
CTCTTCGGCC ACACTGTGCA AATGGTGCAG GCTGCAGCTC TGGATGAGAT GTTTCATCAG 
GGGGAAGCAT CAATTCAGCG CTACCATAAG GCCTTGCTGC TCATGGAGGG TCTGTCGCTT 
CTCCTCACTG AACAGGCTGA CATCCTTAGC ATTAGCAAGT GTAAACAATG CATTGAACGC 
CGTCTCACCG CCTTGCAGTC GGGTCTCTGT GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ulk1b ( XM_002665925.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002665971.2 Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25707
Clone ID Related Accession (Same CDS sequence)	XM_002665925.5 , XM_002665925.6
Accession Version	XM_002665925.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase ULK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002665925.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGCAG GCCGAGTGAG 
TTTTCTGGGA GTAGCTATGG CAACTATGGG CAGTCTGTAC CCATTCCAGT GCCCACACAG 
ATTCACAACT ACCAGCGCAT GGAGCAAAAC TTCCAGTTTC CAGGTCAAGA GGGTTCTCCT 
CGATCTGGTT TGGCTCAGAG ATGCAGTAGT GGTAGTTTTC TGGCCTGTGG AAGGACAGGG 
CCTTCTCCTC CTCGGTCTGG TTCTGCTGTC ATGTCCCGCA GGCTTTCCAC AGGAGGCAGC 
AAACCCTTCC AGCTCTCTCC ACAGGTGGGC ACCATTCCTG AATTGCCAGG CCAAGTTTGC 
CCAGCGAGTG CAGAAGTGGG TCACAGGGGC TCTCGTGGTG GAGAAGCCAA GACTTGGCCT 
CAGCAGCAGG GTCTAGGCAC GCGGCTCAAC AGTGCCCCCT GTTTGCTGGA GGCCGCTGGA 
GGCTGCAGGC AGAAGATCAG GAAGCAGCAC TCAGATCCAG TGGTCGCCCC TCAGGCAAAC 
GTGATGCCCT TCAGGACCCT GCACTCCTCA CCCAGGCTCA GTGAACTTAT GCAACGCAAC 
CCCCTACCAA CTATCCTGGG TTCTCCTTCA AGAGCCATGC CCCCGTTTGA GTTTCCCAAG 
CCTCCGAGTT CCCCAAACAT GGTCACATTT CTGACCCAGC AGGGCCTCAG AACAGCTCAG 
GGAGAGCCTA GTTATTCACC TGCCTACCAG CCAGAGGAGC GGAAAGGATT TGGGAGGTCT 
CAGAGCGCTG GTCGCCTGTC TGATGTGCTG CTGATGGCAG CATTTGGAGG ACAGAGAGGA 
GAACGAGGCA GCATGGAGAA TCTCAACACT GACAGAGCCA TCGACATCAC AGCGCCACCT 
GGTGGTGGGG GCATCGTGGT CGGCTCAGGT AGCCCTGCTC GAGTGGTGTT CACCATTGGT 
TCTCCACCCA GTGGAGCAAC ACCTCCGCAG AATTCTCGCT CAAGAAAGCT CTCCGCAGGT 
TCTTCAAGCT CCGTTAGTCC AGTAGGCTCT CTCACCAGCC GCTACACACA AGCAGGCAAT 
TACATGGACT GCTATGAGGG ATCTTCCAGT CCCCGCTACG GTTTCTCTGA CCCCATCTCA 
GCCGTGACTT TTGAAGCCCC TGAACTACCA GAGGAGACCC TGATGGAACA GGAACACACG 
GAGACTCTTT GCAGGCTCCG CTTCATGCTG GACTTTGCTC GTTGTATGGT GGAGGTAGCA 
GGAGCTCGGA GTGGAGAGAT GGCCCAGACT GAGCTCTCCT CCACCAGCCT GCTCCAACAA 
CAGAGCCTGA TGGCCGACCA GATCAGCTCT CTGAGCCGAG AGTGGAGTTA TGCTGAGCAG 
TTGGTGCTTT ACATGAAGAC CGCAGAGCTT CTGTCATCTT CACTACAAAC AGCCATGGAA 
GGAATCAAGC AGGGAAAATT GTACCCCTCA AGCACCGTCA AACAAGTGGT CAGGAGGTTG 
AATGACCTGT ACAAGTCCAG CGTGATGTCC TGTCGGTCTC TGAGCAATCA GCTGGAGCGA 
TTCTTCACCC GAAAACACAA ACTCATGGAC CACATCAACA GCATCACTGC AGAAAGGCTC 
CTCTTCGGCC ACACTGTGCA AATGGTGCAG GCTGCAGCTC TGGATGAGAT GTTTCATCAG 
GGGGAAGCAT CAATTCAGCG CTACCATAAG GCCTTGCTGC TCATGGAGGG TCTGTCGCTT 
CTCCTCACTG AACAGGCTGA CATCCTTAGC ATTAGCAAGT GTAAACAATG CATTGAACGC 
CGTCTCACCG CCTTGCAGTC GGGTCTCTGT GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665971.2
CDS	550..3585
Translation	METVGKFEFSRKDLIGHGAFAVVFKGRHREKHEWEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALHDFQETASSVYLVMEYCNGGDLADYLHSKGTLSEDTIRVFLQQITGAMRVLQAKGIIHRDLKPQNILLSHPAGRKSHFNNTCIKIADFGFARYLQNNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVFQCLTGKAPFQASSPQDLRLFYEKNKTLSPNIPRETSTHLRHLLLGLLQRNHKDRMDFDEFFRHPFLEASSSMKKSTPVTVTCFPNSASASSCSSSSTSHLASPPQSLAEIQHVRAKALASPTQDSPGYLLKDSGGGGGGSSSKNSSCDTDDFVMVPAHFPSELTCDMPTGKVLQDSLMYSGSSLLASGGQCSQGKTPPRSPSFSSSPGPSGRPSEFSGSSYGNYGQSVPIPVPTQIHNYQRMEQNFQFPGQEGSPRSGLAQRCSSGSFLACGRTGPSPPRSGSAVMSRRLSTGGSKPFQLSPQVGTIPELPGQVCPASAEVGHRGSRGGEAKTWPQQQGLGTRLNSAPCLLEAAGGCRQKIRKQHSDPVVAPQANVMPFRTLHSSPRLSELMQRNPLPTILGSPSRAMPPFEFPKPPSSPNMVTFLTQQGLRTAQGEPSYSPAYQPEERKGFGRSQSAGRLSDVLLMAAFGGQRGERGSMENLNTDRAIDITAPPGGGGIVVGSGSPARVVFTIGSPPSGATPPQNSRSRKLSAGSSSSVSPVGSLTSRYTQAGNYMDCYEGSSSPRYGFSDPISAVTFEAPELPEETLMEQEHTETLCRLRFMLDFARCMVEVAGARSGEMAQTELSSTSLLQQQSLMADQISSLSREWSYAEQLVLYMKTAELLSSSLQTAMEGIKQGKLYPSSTVKQVVRRLNDLYKSSVMSCRSLSNQLERFFTRKHKLMDHINSITAERLLFGHTVQMVQAAALDEMFHQGEASIQRYHKALLLMEGLSLLLTEQADILSISKCKQCIERRLTALQSGLCV

Target ORF information:

RefSeq Version	XM_002665925.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665925.6

ORF Insert Sequence:

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGCAG GCCGAGTGAG 
TTTTCTGGGA GTAGCTATGG CAACTATGGG CAGTCTGTAC CCATTCCAGT GCCCACACAG 
ATTCACAACT ACCAGCGCAT GGAGCAAAAC TTCCAGTTTC CAGGTCAAGA GGGTTCTCCT 
CGATCTGGTT TGGCTCAGAG ATGCAGTAGT GGTAGTTTTC TGGCCTGTGG AAGGACAGGG 
CCTTCTCCTC CTCGGTCTGG TTCTGCTGTC ATGTCCCGCA GGCTTTCCAC AGGAGGCAGC 
AAACCCTTCC AGCTCTCTCC ACAGGTGGGC ACCATTCCTG AATTGCCAGG CCAAGTTTGC 
CCAGCGAGTG CAGAAGTGGG TCACAGGGGC TCTCGTGGTG GAGAAGCCAA GACTTGGCCT 
CAGCAGCAGG GTCTAGGCAC GCGGCTCAAC AGTGCCCCCT GTTTGCTGGA GGCCGCTGGA 
GGCTGCAGGC AGAAGATCAG GAAGCAGCAC TCAGATCCAG TGGTCGCCCC TCAGGCAAAC 
GTGATGCCCT TCAGGACCCT GCACTCCTCA CCCAGGCTCA GTGAACTTAT GCAACGCAAC 
CCCCTACCAA CTATCCTGGG TTCTCCTTCA AGAGCCATGC CCCCGTTTGA GTTTCCCAAG 
CCTCCGAGTT CCCCAAACAT GGTCACATTT CTGACCCAGC AGGGCCTCAG AACAGCTCAG 
GGAGAGCCTA GTTATTCACC TGCCTACCAG CCAGAGGAGC GGAAAGGATT TGGGAGGTCT 
CAGAGCGCTG GTCGCCTGTC TGATGTGCTG CTGATGGCAG CATTTGGAGG ACAGAGAGGA 
GAACGAGGCA GCATGGAGAA TCTCAACACT GACAGAGCCA TCGACATCAC AGCGCCACCT 
GGTGGTGGGG GCATCGTGGT CGGCTCAGGT AGCCCTGCTC GAGTGGTGTT CACCATTGGT 
TCTCCACCCA GTGGAGCAAC ACCTCCGCAG AATTCTCGCT CAAGAAAGCT CTCCGCAGGT 
TCTTCAAGCT CCGTTAGTCC AGTAGGCTCT CTCACCAGCC GCTACACACA AGCAGGCAAT 
TACATGGACT GCTATGAGGG ATCTTCCAGT CCCCGCTACG GTTTCTCTGA CCCCATCTCA 
GCCGTGACTT TTGAAGCCCC TGAACTACCA GAGGAGACCC TGATGGAACA GGAACACACG 
GAGACTCTTT GCAGGCTCCG CTTCATGCTG GACTTTGCTC GTTGTATGGT GGAGGTAGCA 
GGAGCTCGGA GTGGAGAGAT GGCCCAGACT GAGCTCTCCT CCACCAGCCT GCTCCAACAA 
CAGAGCCTGA TGGCCGACCA GATCAGCTCT CTGAGCCGAG AGTGGAGTTA TGCTGAGCAG 
TTGGTGCTTT ACATGAAGAC CGCAGAGCTT CTGTCATCTT CACTACAAAC AGCCATGGAA 
GGAATCAAGC AGGGAAAATT GTACCCCTCA AGCACCGTCA AACAAGTGGT CAGGAGGTTG 
AATGACCTGT ACAAGTCCAG CGTGATGTCC TGTCGGTCTC TGAGCAATCA GCTGGAGCGA 
TTCTTCACCC GAAAACACAA ACTCATGGAC CACATCAACA GCATCACTGC AGAAAGGCTC 
CTCTTCGGCC ACACTGTGCA AATGGTGCAG GCTGCAGCTC TGGATGAGAT GTTTCATCAG 
GGGGAAGCAT CAATTCAGCG CTACCATAAG GCCTTGCTGC TCATGGAGGG TCTGTCGCTT 
CTCCTCACTG AACAGGCTGA CATCCTTAGC ATTAGCAAGT GTAAACAATG CATTGAACGC 
CGTCTCACCG CCTTGCAGTC GGGTCTCTGT GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ulk1b ( XM_005161121.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161178.1 Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa25708
Clone ID Related Accession (Same CDS sequence)	XM_005161121.4 , XM_005161121.3
Accession Version	XM_005161121.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase ULK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161121.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGGCC GAGTGAGTTT 
TCTGGGAGTA GCTATGGCAA CTATGGGCAG TCTGTACCCA TTCCAGTGCC CACACAGATT 
CACAACTACC AGCGCATGGA GCAAAACTTC CAGTTTCCAG GTCAAGAGGG TTCTCCTCGA 
TCTGGTTTGG CTCAGAGATG CAGTAGTGGT AGTTTTCTGG CCTGTGGAAG GACAGGGCCT 
TCTCCTCCTC GGTCTGGTTC TGCTGTCATG TCCCGCAGGC TTTCCACAGG AGGCAGCAAA 
CCCTTCCAGC TCTCTCCACA GGTGGGCACC ATTCCTGAAT TGCCAGGCCA AGTTTGCCCA 
GCGAGTGCAG AAGTGGGTCA CAGGGGCTCT CGTGGTGGAG AAGCCAAGAC TTGGCCTCAG 
CAGCAGGGTC TAGGCACGCG GCTCAACAGT GCCCCCTGTT TGCTGGAGGC CGCTGGAGGC 
TGCAGGCAGA AGATCAGGAA GCAGCACTCA GATCCAGTGG TCGCCCCTCA GGCAAACGTG 
ATGCCCTTCA GGACCCTGCA CTCCTCACCC AGGCTCAGTG AACTTATGCA ACGCAACCCC 
CTACCAACTA TCCTGGGTTC TCCTTCAAGA GCCATGCCCC CGTTTGAGTT TCCCAAGCCT 
CCGAGTTCCC CAAACATGGT CACATTTCTG ACCCAGCAGG GCCTCAGAAC AGCTCAGGGA 
GAGCCTAGTT ATTCACCTGC CTACCAGCCA GAGGAGCGGA AAGGATTTGG GAGGTCTCAG 
AGCGCTGGTC GCCTGTCTGA TGTGCTGCTG ATGGCAGCAT TTGGAGGACA GAGAGGAGAA 
CGAGGCAGCA TGGAGAATCT CAACACTGAC AGAGCCATCG ACATCACAGC GCCACCTGGT 
GGTGGGGGCA TCGTGGTCGG CTCAGGTAGC CCTGCTCGAG TGGTGTTCAC CATTGGTTCT 
CCACCCAGTG GAGCAACACC TCCGCAGAAT TCTCGCTCAA GAAAGCTCTC CGCAGGTTCT 
TCAAGCTCCG TTAGTCCAGT AGGCTCTCTC ACCAGCCGCT ACACACAAGC AGGCAATTAC 
ATGGACTGCT ATGAGGGATC TTCCAGTCCC CGCTACGGTT TCTCTGACCC CATCTCAGCC 
GTGACTTTTG AAGCCCCTGA ACTACCAGAG GAGACCCTGA TGGAACAGGA ACACACGGAG 
ACTCTTTGCA GGCTCCGCTT CATGCTGGAC TTTGCTCGTT GTATGGTGGA GGTAGCAGGA 
GCTCGGAGTG GAGAGATGGC CCAGACTGAG CTCTCCTCCA CCAGCCTGCT CCAACAACAG 
AGCCTGATGG CCGACCAGAT CAGCTCTCTG AGCCGAGAGT GGAGTTATGC TGAGCAGTTG 
GTGCTTTACA TGAAGACCGC AGAGCTTCTG TCATCTTCAC TACAAACAGC CATGGAAGGA 
ATCAAGCAGG GAAAATTGTA CCCCTCAAGC ACCGTCAAAC AAGTGGTCAG GAGGTTGAAT 
GACCTGTACA AGTCCAGCGT GATGTCCTGT CGGTCTCTGA GCAATCAGCT GGAGCGATTC 
TTCACCCGAA AACACAAACT CATGGACCAC ATCAACAGCA TCACTGCAGA AAGGCTCCTC 
TTCGGCCACA CTGTGCAAAT GGTGCAGGCT GCAGCTCTGG ATGAGATGTT TCATCAGGGG 
GAAGCATCAA TTCAGCGCTA CCATAAGGCC TTGCTGCTCA TGGAGGGTCT GTCGCTTCTC 
CTCACTGAAC AGGCTGACAT CCTTAGCATT AGCAAGTGTA AACAATGCAT TGAACGCCGT 
CTCACCGCCT TGCAGTCGGG TCTCTGTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161178.1
CDS	550..3582
Translation	METVGKFEFSRKDLIGHGAFAVVFKGRHREKHEWEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALHDFQETASSVYLVMEYCNGGDLADYLHSKGTLSEDTIRVFLQQITGAMRVLQAKGIIHRDLKPQNILLSHPAGRKSHFNNTCIKIADFGFARYLQNNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVFQCLTGKAPFQASSPQDLRLFYEKNKTLSPNIPRETSTHLRHLLLGLLQRNHKDRMDFDEFFRHPFLEASSSMKKSTPVTVTCFPNSASASSCSSSSTSHLASPPQSLAEIQHVRAKALASPTQDSPGYLLKDSGGGGGGSSSKNSSCDTDDFVMVPAHFPSELTCDMPTGKVLQDSLMYSGSSLLASGGQCSQGKTPPRSPSFSSSPGPSGPSEFSGSSYGNYGQSVPIPVPTQIHNYQRMEQNFQFPGQEGSPRSGLAQRCSSGSFLACGRTGPSPPRSGSAVMSRRLSTGGSKPFQLSPQVGTIPELPGQVCPASAEVGHRGSRGGEAKTWPQQQGLGTRLNSAPCLLEAAGGCRQKIRKQHSDPVVAPQANVMPFRTLHSSPRLSELMQRNPLPTILGSPSRAMPPFEFPKPPSSPNMVTFLTQQGLRTAQGEPSYSPAYQPEERKGFGRSQSAGRLSDVLLMAAFGGQRGERGSMENLNTDRAIDITAPPGGGGIVVGSGSPARVVFTIGSPPSGATPPQNSRSRKLSAGSSSSVSPVGSLTSRYTQAGNYMDCYEGSSSPRYGFSDPISAVTFEAPELPEETLMEQEHTETLCRLRFMLDFARCMVEVAGARSGEMAQTELSSTSLLQQQSLMADQISSLSREWSYAEQLVLYMKTAELLSSSLQTAMEGIKQGKLYPSSTVKQVVRRLNDLYKSSVMSCRSLSNQLERFFTRKHKLMDHINSITAERLLFGHTVQMVQAAALDEMFHQGEASIQRYHKALLLMEGLSLLLTEQADILSISKCKQCIERRLTALQSGLCV

Target ORF information:

RefSeq Version	XM_005161121.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161121.4

ORF Insert Sequence:

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGGCC GAGTGAGTTT 
TCTGGGAGTA GCTATGGCAA CTATGGGCAG TCTGTACCCA TTCCAGTGCC CACACAGATT 
CACAACTACC AGCGCATGGA GCAAAACTTC CAGTTTCCAG GTCAAGAGGG TTCTCCTCGA 
TCTGGTTTGG CTCAGAGATG CAGTAGTGGT AGTTTTCTGG CCTGTGGAAG GACAGGGCCT 
TCTCCTCCTC GGTCTGGTTC TGCTGTCATG TCCCGCAGGC TTTCCACAGG AGGCAGCAAA 
CCCTTCCAGC TCTCTCCACA GGTGGGCACC ATTCCTGAAT TGCCAGGCCA AGTTTGCCCA 
GCGAGTGCAG AAGTGGGTCA CAGGGGCTCT CGTGGTGGAG AAGCCAAGAC TTGGCCTCAG 
CAGCAGGGTC TAGGCACGCG GCTCAACAGT GCCCCCTGTT TGCTGGAGGC CGCTGGAGGC 
TGCAGGCAGA AGATCAGGAA GCAGCACTCA GATCCAGTGG TCGCCCCTCA GGCAAACGTG 
ATGCCCTTCA GGACCCTGCA CTCCTCACCC AGGCTCAGTG AACTTATGCA ACGCAACCCC 
CTACCAACTA TCCTGGGTTC TCCTTCAAGA GCCATGCCCC CGTTTGAGTT TCCCAAGCCT 
CCGAGTTCCC CAAACATGGT CACATTTCTG ACCCAGCAGG GCCTCAGAAC AGCTCAGGGA 
GAGCCTAGTT ATTCACCTGC CTACCAGCCA GAGGAGCGGA AAGGATTTGG GAGGTCTCAG 
AGCGCTGGTC GCCTGTCTGA TGTGCTGCTG ATGGCAGCAT TTGGAGGACA GAGAGGAGAA 
CGAGGCAGCA TGGAGAATCT CAACACTGAC AGAGCCATCG ACATCACAGC GCCACCTGGT 
GGTGGGGGCA TCGTGGTCGG CTCAGGTAGC CCTGCTCGAG TGGTGTTCAC CATTGGTTCT 
CCACCCAGTG GAGCAACACC TCCGCAGAAT TCTCGCTCAA GAAAGCTCTC CGCAGGTTCT 
TCAAGCTCCG TTAGTCCAGT AGGCTCTCTC ACCAGCCGCT ACACACAAGC AGGCAATTAC 
ATGGACTGCT ATGAGGGATC TTCCAGTCCC CGCTACGGTT TCTCTGACCC CATCTCAGCC 
GTGACTTTTG AAGCCCCTGA ACTACCAGAG GAGACCCTGA TGGAACAGGA ACACACGGAG 
ACTCTTTGCA GGCTCCGCTT CATGCTGGAC TTTGCTCGTT GTATGGTGGA GGTAGCAGGA 
GCTCGGAGTG GAGAGATGGC CCAGACTGAG CTCTCCTCCA CCAGCCTGCT CCAACAACAG 
AGCCTGATGG CCGACCAGAT CAGCTCTCTG AGCCGAGAGT GGAGTTATGC TGAGCAGTTG 
GTGCTTTACA TGAAGACCGC AGAGCTTCTG TCATCTTCAC TACAAACAGC CATGGAAGGA 
ATCAAGCAGG GAAAATTGTA CCCCTCAAGC ACCGTCAAAC AAGTGGTCAG GAGGTTGAAT 
GACCTGTACA AGTCCAGCGT GATGTCCTGT CGGTCTCTGA GCAATCAGCT GGAGCGATTC 
TTCACCCGAA AACACAAACT CATGGACCAC ATCAACAGCA TCACTGCAGA AAGGCTCCTC 
TTCGGCCACA CTGTGCAAAT GGTGCAGGCT GCAGCTCTGG ATGAGATGTT TCATCAGGGG 
GAAGCATCAA TTCAGCGCTA CCATAAGGCC TTGCTGCTCA TGGAGGGTCT GTCGCTTCTC 
CTCACTGAAC AGGCTGACAT CCTTAGCATT AGCAAGTGTA AACAATGCAT TGAACGCCGT 
CTCACCGCCT TGCAGTCGGG TCTCTGTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ulk1b ( XM_005161121.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161178.1 Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X2, mRNA.

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CloneID	ODa25708
Clone ID Related Accession (Same CDS sequence)	XM_005161121.4 , XM_005161121.3
Accession Version	XM_005161121.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase ULK1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGGCC GAGTGAGTTT 
TCTGGGAGTA GCTATGGCAA CTATGGGCAG TCTGTACCCA TTCCAGTGCC CACACAGATT 
CACAACTACC AGCGCATGGA GCAAAACTTC CAGTTTCCAG GTCAAGAGGG TTCTCCTCGA 
TCTGGTTTGG CTCAGAGATG CAGTAGTGGT AGTTTTCTGG CCTGTGGAAG GACAGGGCCT 
TCTCCTCCTC GGTCTGGTTC TGCTGTCATG TCCCGCAGGC TTTCCACAGG AGGCAGCAAA 
CCCTTCCAGC TCTCTCCACA GGTGGGCACC ATTCCTGAAT TGCCAGGCCA AGTTTGCCCA 
GCGAGTGCAG AAGTGGGTCA CAGGGGCTCT CGTGGTGGAG AAGCCAAGAC TTGGCCTCAG 
CAGCAGGGTC TAGGCACGCG GCTCAACAGT GCCCCCTGTT TGCTGGAGGC CGCTGGAGGC 
TGCAGGCAGA AGATCAGGAA GCAGCACTCA GATCCAGTGG TCGCCCCTCA GGCAAACGTG 
ATGCCCTTCA GGACCCTGCA CTCCTCACCC AGGCTCAGTG AACTTATGCA ACGCAACCCC 
CTACCAACTA TCCTGGGTTC TCCTTCAAGA GCCATGCCCC CGTTTGAGTT TCCCAAGCCT 
CCGAGTTCCC CAAACATGGT CACATTTCTG ACCCAGCAGG GCCTCAGAAC AGCTCAGGGA 
GAGCCTAGTT ATTCACCTGC CTACCAGCCA GAGGAGCGGA AAGGATTTGG GAGGTCTCAG 
AGCGCTGGTC GCCTGTCTGA TGTGCTGCTG ATGGCAGCAT TTGGAGGACA GAGAGGAGAA 
CGAGGCAGCA TGGAGAATCT CAACACTGAC AGAGCCATCG ACATCACAGC GCCACCTGGT 
GGTGGGGGCA TCGTGGTCGG CTCAGGTAGC CCTGCTCGAG TGGTGTTCAC CATTGGTTCT 
CCACCCAGTG GAGCAACACC TCCGCAGAAT TCTCGCTCAA GAAAGCTCTC CGCAGGTTCT 
TCAAGCTCCG TTAGTCCAGT AGGCTCTCTC ACCAGCCGCT ACACACAAGC AGGCAATTAC 
ATGGACTGCT ATGAGGGATC TTCCAGTCCC CGCTACGGTT TCTCTGACCC CATCTCAGCC 
GTGACTTTTG AAGCCCCTGA ACTACCAGAG GAGACCCTGA TGGAACAGGA ACACACGGAG 
ACTCTTTGCA GGCTCCGCTT CATGCTGGAC TTTGCTCGTT GTATGGTGGA GGTAGCAGGA 
GCTCGGAGTG GAGAGATGGC CCAGACTGAG CTCTCCTCCA CCAGCCTGCT CCAACAACAG 
AGCCTGATGG CCGACCAGAT CAGCTCTCTG AGCCGAGAGT GGAGTTATGC TGAGCAGTTG 
GTGCTTTACA TGAAGACCGC AGAGCTTCTG TCATCTTCAC TACAAACAGC CATGGAAGGA 
ATCAAGCAGG GAAAATTGTA CCCCTCAAGC ACCGTCAAAC AAGTGGTCAG GAGGTTGAAT 
GACCTGTACA AGTCCAGCGT GATGTCCTGT CGGTCTCTGA GCAATCAGCT GGAGCGATTC 
TTCACCCGAA AACACAAACT CATGGACCAC ATCAACAGCA TCACTGCAGA AAGGCTCCTC 
TTCGGCCACA CTGTGCAAAT GGTGCAGGCT GCAGCTCTGG ATGAGATGTT TCATCAGGGG 
GAAGCATCAA TTCAGCGCTA CCATAAGGCC TTGCTGCTCA TGGAGGGTCT GTCGCTTCTC 
CTCACTGAAC AGGCTGACAT CCTTAGCATT AGCAAGTGTA AACAATGCAT TGAACGCCGT 
CTCACCGCCT TGCAGTCGGG TCTCTGTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161178.1
CDS	531..3563
Translation	METVGKFEFSRKDLIGHGAFAVVFKGRHREKHEWEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALHDFQETASSVYLVMEYCNGGDLADYLHSKGTLSEDTIRVFLQQITGAMRVLQAKGIIHRDLKPQNILLSHPAGRKSHFNNTCIKIADFGFARYLQNNMMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVFQCLTGKAPFQASSPQDLRLFYEKNKTLSPNIPRETSTHLRHLLLGLLQRNHKDRMDFDEFFRHPFLEASSSMKKSTPVTVTCFPNSASASSCSSSSTSHLASPPQSLAEIQHVRAKALASPTQDSPGYLLKDSGGGGGGSSSKNSSCDTDDFVMVPAHFPSELTCDMPTGKVLQDSLMYSGSSLLASGGQCSQGKTPPRSPSFSSSPGPSGPSEFSGSSYGNYGQSVPIPVPTQIHNYQRMEQNFQFPGQEGSPRSGLAQRCSSGSFLACGRTGPSPPRSGSAVMSRRLSTGGSKPFQLSPQVGTIPELPGQVCPASAEVGHRGSRGGEAKTWPQQQGLGTRLNSAPCLLEAAGGCRQKIRKQHSDPVVAPQANVMPFRTLHSSPRLSELMQRNPLPTILGSPSRAMPPFEFPKPPSSPNMVTFLTQQGLRTAQGEPSYSPAYQPEERKGFGRSQSAGRLSDVLLMAAFGGQRGERGSMENLNTDRAIDITAPPGGGGIVVGSGSPARVVFTIGSPPSGATPPQNSRSRKLSAGSSSSVSPVGSLTSRYTQAGNYMDCYEGSSSPRYGFSDPISAVTFEAPELPEETLMEQEHTETLCRLRFMLDFARCMVEVAGARSGEMAQTELSSTSLLQQQSLMADQISSLSREWSYAEQLVLYMKTAELLSSSLQTAMEGIKQGKLYPSSTVKQVVRRLNDLYKSSVMSCRSLSNQLERFFTRKHKLMDHINSITAERLLFGHTVQMVQAAALDEMFHQGEASIQRYHKALLLMEGLSLLLTEQADILSISKCKQCIERRLTALQSGLCV

Target ORF information:

RefSeq Version	XM_005161121.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio unc-51 like autophagy activating kinase 1 (ulk1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161121.3

ORF Insert Sequence:

ATGGAGACTG TCGGGAAATT CGAGTTCAGC AGGAAGGACC TCATAGGACA CGGTGCCTTC 
GCAGTGGTTT TCAAAGGCAG ACATCGAGAG AAGCATGAAT GGGAGGTTGC CGTCAAATGC 
ATAAACAAGA AGAATTTGGC CAAATCGCAG ACTCTTCTGG GCAAAGAAAT CAAGATATTG 
AAGGAACTGA AACATGAAAA CATCGTTGCA TTGCACGACT TTCAGGAAAC GGCTAGCTCC 
GTGTACCTTG TAATGGAGTA CTGCAACGGG GGAGACCTGG CTGACTACTT GCACTCAAAG 
GGCACTTTAA GTGAAGACAC CATCCGAGTA TTCCTACAGC AGATCACAGG GGCCATGAGG 
GTTCTGCAGG CCAAAGGGAT CATCCACAGA GACCTGAAAC CCCAAAACAT CCTGTTGTCC 
CATCCTGCAG GCCGAAAGTC TCACTTCAAC AACACCTGCA TTAAGATAGC TGACTTTGGC 
TTTGCGAGGT ACCTCCAGAA CAACATGATG GCTGCCACTC TCTGTGGGTC TCCGATGTAC 
ATGGCTCCTG AAGTTATCAT GTCACAAAAC TATGATGCCA AGGCTGACCT GTGGAGCATA 
GGGACCATTG TGTTTCAGTG TCTGACTGGG AAGGCTCCAT TTCAGGCCAG CAGTCCCCAG 
GATCTCCGCT TGTTTTATGA GAAAAATAAA ACTCTTAGCC CAAACATTCC CAGAGAAACT 
TCCACCCATC TCAGACACCT GCTGCTCGGC CTCCTGCAGA GGAACCATAA GGACCGAATG 
GACTTCGATG AATTCTTCAG ACATCCATTT TTGGAAGCAA GCTCATCTAT GAAGAAGTCT 
ACACCAGTGA CCGTGACATG CTTTCCCAAT TCAGCCTCTG CAAGCTCATG CAGCAGTTCA 
TCTACTTCAC ACCTGGCCTC TCCTCCTCAA TCGCTTGCTG AGATCCAGCA TGTCCGTGCA 
AAGGCGTTAG CCTCCCCTAC CCAGGATTCT CCAGGATATC TGCTGAAAGA CTCAGGAGGA 
GGTGGAGGAG GCAGCAGTAG CAAAAACTCA TCCTGTGACA CTGATGATTT CGTCATGGTT 
CCAGCTCATT TCCCCAGTGA GCTTACCTGT GACATGCCAA CTGGAAAGGT TCTCCAGGAC 
AGTTTGATGT ATAGCGGGAG TTCTCTTCTG GCGTCCGGAG GTCAGTGCAG TCAAGGAAAG 
ACTCCGCCAC GCTCTCCATC ATTCAGCAGC TCTCCAGGAC CCAGCGGGCC GAGTGAGTTT 
TCTGGGAGTA GCTATGGCAA CTATGGGCAG TCTGTACCCA TTCCAGTGCC CACACAGATT 
CACAACTACC AGCGCATGGA GCAAAACTTC CAGTTTCCAG GTCAAGAGGG TTCTCCTCGA 
TCTGGTTTGG CTCAGAGATG CAGTAGTGGT AGTTTTCTGG CCTGTGGAAG GACAGGGCCT 
TCTCCTCCTC GGTCTGGTTC TGCTGTCATG TCCCGCAGGC TTTCCACAGG AGGCAGCAAA 
CCCTTCCAGC TCTCTCCACA GGTGGGCACC ATTCCTGAAT TGCCAGGCCA AGTTTGCCCA 
GCGAGTGCAG AAGTGGGTCA CAGGGGCTCT CGTGGTGGAG AAGCCAAGAC TTGGCCTCAG 
CAGCAGGGTC TAGGCACGCG GCTCAACAGT GCCCCCTGTT TGCTGGAGGC CGCTGGAGGC 
TGCAGGCAGA AGATCAGGAA GCAGCACTCA GATCCAGTGG TCGCCCCTCA GGCAAACGTG 
ATGCCCTTCA GGACCCTGCA CTCCTCACCC AGGCTCAGTG AACTTATGCA ACGCAACCCC 
CTACCAACTA TCCTGGGTTC TCCTTCAAGA GCCATGCCCC CGTTTGAGTT TCCCAAGCCT 
CCGAGTTCCC CAAACATGGT CACATTTCTG ACCCAGCAGG GCCTCAGAAC AGCTCAGGGA 
GAGCCTAGTT ATTCACCTGC CTACCAGCCA GAGGAGCGGA AAGGATTTGG GAGGTCTCAG 
AGCGCTGGTC GCCTGTCTGA TGTGCTGCTG ATGGCAGCAT TTGGAGGACA GAGAGGAGAA 
CGAGGCAGCA TGGAGAATCT CAACACTGAC AGAGCCATCG ACATCACAGC GCCACCTGGT 
GGTGGGGGCA TCGTGGTCGG CTCAGGTAGC CCTGCTCGAG TGGTGTTCAC CATTGGTTCT 
CCACCCAGTG GAGCAACACC TCCGCAGAAT TCTCGCTCAA GAAAGCTCTC CGCAGGTTCT 
TCAAGCTCCG TTAGTCCAGT AGGCTCTCTC ACCAGCCGCT ACACACAAGC AGGCAATTAC 
ATGGACTGCT ATGAGGGATC TTCCAGTCCC CGCTACGGTT TCTCTGACCC CATCTCAGCC 
GTGACTTTTG AAGCCCCTGA ACTACCAGAG GAGACCCTGA TGGAACAGGA ACACACGGAG 
ACTCTTTGCA GGCTCCGCTT CATGCTGGAC TTTGCTCGTT GTATGGTGGA GGTAGCAGGA 
GCTCGGAGTG GAGAGATGGC CCAGACTGAG CTCTCCTCCA CCAGCCTGCT CCAACAACAG 
AGCCTGATGG CCGACCAGAT CAGCTCTCTG AGCCGAGAGT GGAGTTATGC TGAGCAGTTG 
GTGCTTTACA TGAAGACCGC AGAGCTTCTG TCATCTTCAC TACAAACAGC CATGGAAGGA 
ATCAAGCAGG GAAAATTGTA CCCCTCAAGC ACCGTCAAAC AAGTGGTCAG GAGGTTGAAT 
GACCTGTACA AGTCCAGCGT GATGTCCTGT CGGTCTCTGA GCAATCAGCT GGAGCGATTC 
TTCACCCGAA AACACAAACT CATGGACCAC ATCAACAGCA TCACTGCAGA AAGGCTCCTC 
TTCGGCCACA CTGTGCAAAT GGTGCAGGCT GCAGCTCTGG ATGAGATGTT TCATCAGGGG 
GAAGCATCAA TTCAGCGCTA CCATAAGGCC TTGCTGCTCA TGGAGGGTCT GTCGCTTCTC 
CTCACTGAAC AGGCTGACAT CCTTAGCATT AGCAAGTGTA AACAATGCAT TGAACGCCGT 
CTCACCGCCT TGCAGTCGGG TCTCTGTGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ulk1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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