serpinh1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	serpinh1a
Entrez Gene ID	555328
Full Name	serpin peptidase inhibitor, clade H (heat shock protein 47), member 1a
Synonyms	wu:fa97d12,wu:fc59b06,zgc:171630
General protein information	Preferred Names serpin peptidase inhibitor, clade H (heat shock protein 47), member 1a Names serpin H1 hsp47
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110374.2	NP_001103844.1	serpin H1 precursor

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	SERPINH1	XP_001084827.2
Rattus norvegicus (Norway rat)	Serpinh1	NP_058869.1
Gallus gallus (chicken)	SERPINH1	NP_990622.1
Mus musculus (house mouse)	Serpinh1	NP_001104513.1
Danio rerio (zebrafish)	serpinh1b	NP_571279.1
Xenopus tropicalis (tropical clawed frog)	LOC100490491	XP_004912280.1
Homo sapiens (human)	SERPINH1	NP_001193943.1
Pan troglodytes (chimpanzee)	SERPINH1	NP_001233539.1
Canis lupus familiaris (dog)	SERPINH1	NP_001159360.1
Bos taurus (cattle)	SERPINH1	NP_001039528.1
Danio rerio (zebrafish)	serpinh1a	NP_001103844.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome.
Weh E, Takeuchi H, Muheisen S, Haltiwanger RS, Semina EV
PloS one12(9)e0184903(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Fructose leads to hepatic steatosis in zebrafish that is reversed by mechanistic target of rapamycin (mTOR) inhibition.
Sapp V, Gaffney L, EauClaire SF, Matthews RP
Hepatology (Baltimore, Md.)60(5)1581-92(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following serpinh1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the serpinh1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01097	NM_001110374.2 Latest version!	Danio rerio serpin peptidase inhibitor, clade H (heat shock protein 47), member 1a (serpinh1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

serpinh1a ( NM_001110374.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103844.1 Danio rerio serpin peptidase inhibitor, clade H (heat shock protein 47), member 1a (serpinh1a), mRNA.

Price & Availability↑

CloneID	ODa01097
Clone ID Related Accession (Same CDS sequence)	NM_001110374.2
Accession Version	NM_001110374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	serpin H1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX000451.4. On Apr 18, 2014 this sequence version replaced NM_001110374.1. ##Evidence-Data-START## Transcript exon combination :: BC154462.1, GFIL01034903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTTGGCGT CAAACGTGCT TCTCCTGTGC CTACTGGCCA CTGTGAGCGC AAACAAGACC 
CTGAGCAGCA TTGCAACCAC GCTAGCCGAC AACAGCGCCA CACTGGCCTT CAACCTGTAT 
CAAAACATGG CGAAAGATAA GGACATCGAG AACATCCTGA TTTCCCCAGT GGTAGTGGCC 
TCATCGCTGG GTCTGGTGGC TCTTGGAGGA AAATCCAACA CTGCTTCTCA GGTCAAAACC 
GTCTTGAGCG CCGCTTCAGT GAAGGATGAG CAACTCCATT CTGGGCTTTC GGAGCTCCTC 
ACCGAGGTCA GCAACCCAAA GGCACGAAAC GTCACCTGGA AGATCAGCAA CCGCTTCTAC 
GGGCCCAGTT CTGTTAGCTT CGTTGATGAC TTTCTGAAAA GCAGCAAGAA GCATTACAAT 
TACGACCACT CCAAAATTAA CTTCCGCGAC AAACGCAGTG CGGTGAAAGC CATCAACGAC 
TGGGCATCGA AGTCCACCGA TGGAAAGCTG CCGGAGGTGA CCAAAGATGT GGAGAAGACA 
GATGGAGCCA TGATCATCAA TGCCATGTTT TATAAACCAC ACTGGAATGA GCAGTTTCAT 
CATAAGATGG TGGACAATCG TGGTTTCTTA GTGCACCGGT CATTCACCGT CTCCGTACCC 
ATGATGCACC GCACAGGCAT TTATGGCTTC CTTGATGACA CAACCAACAA ACTTTTAGTT 
CTGGAAATGC CGCTGGCCCA TAAGATGTCA TCTTTAGTCC TTATCATGCC ATACCACGTA 
GAATCACTGG AAAGAGTGGA GAAACTGCTG ACACGTCAAC AACTGAACAC CTGGGTCAGT 
GCAATGGAAC AGAAAGCAGT AGCAATCTCT CTGCCAAAGG TCAGCATGGA GGTCAGCCAC 
AACCTGCAGA AACATCTCGC TGAGCTGGGT CTGACTGAAG CTGTGGATAA GGCCAAGGCT 
GATCTGTCCA ACATCTCAGG GAAGAAAGAC CTCTATTTGT CCAATGTTTT CCATGCCTCA 
GCCATGGAGT GGGACACAGA GGGAAATCCG CCTGATACTA GCATCTTTGG TACAGACCAG 
CTGAAAAATC CCAAACTCTT CTACGCCGAC CATCCTTTTG TCTTCCTGGT GAAAGACAAC 
AAGACAAACT CTATTCTTTT CATGGGCAGG CTGATCCGGC CGAAGGGTGA TAAAATGAGG 
GATGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103844.1
CDS	163..1374
Translation	MLASNVLLLCLLATVSANKTLSSIATTLADNSATLAFNLYQNMAKDKDIENILISPVVVASSLGLVALGGKSNTASQVKTVLSAASVKDEQLHSGLSELLTEVSNPKARNVTWKISNRFYGPSSVSFVDDFLKSSKKHYNYDHSKINFRDKRSAVKAINDWASKSTDGKLPEVTKDVEKTDGAMIINAMFYKPHWNEQFHHKMVDNRGFLVHRSFTVSVPMMHRTGIYGFLDDTTNKLLVLEMPLAHKMSSLVLIMPYHVESLERVEKLLTRQQLNTWVSAMEQKAVAISLPKVSMEVSHNLQKHLAELGLTEAVDKAKADLSNISGKKDLYLSNVFHASAMEWDTEGNPPDTSIFGTDQLKNPKLFYADHPFVFLVKDNKTNSILFMGRLIRPKGDKMRDEL

Target ORF information:

RefSeq Version	NM_001110374.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio serpin peptidase inhibitor, clade H (heat shock protein 47), member 1a (serpinh1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110374.2

ORF Insert Sequence:

ATGTTGGCGT CAAACGTGCT TCTCCTGTGC CTACTGGCCA CTGTGAGCGC AAACAAGACC 
CTGAGCAGCA TTGCAACCAC GCTAGCCGAC AACAGCGCCA CACTGGCCTT CAACCTGTAT 
CAAAACATGG CGAAAGATAA GGACATCGAG AACATCCTGA TTTCCCCAGT GGTAGTGGCC 
TCATCGCTGG GTCTGGTGGC TCTTGGAGGA AAATCCAACA CTGCTTCTCA GGTCAAAACC 
GTCTTGAGCG CCGCTTCAGT GAAGGATGAG CAACTCCATT CTGGGCTTTC GGAGCTCCTC 
ACCGAGGTCA GCAACCCAAA GGCACGAAAC GTCACCTGGA AGATCAGCAA CCGCTTCTAC 
GGGCCCAGTT CTGTTAGCTT CGTTGATGAC TTTCTGAAAA GCAGCAAGAA GCATTACAAT 
TACGACCACT CCAAAATTAA CTTCCGCGAC AAACGCAGTG CGGTGAAAGC CATCAACGAC 
TGGGCATCGA AGTCCACCGA TGGAAAGCTG CCGGAGGTGA CCAAAGATGT GGAGAAGACA 
GATGGAGCCA TGATCATCAA TGCCATGTTT TATAAACCAC ACTGGAATGA GCAGTTTCAT 
CATAAGATGG TGGACAATCG TGGTTTCTTA GTGCACCGGT CATTCACCGT CTCCGTACCC 
ATGATGCACC GCACAGGCAT TTATGGCTTC CTTGATGACA CAACCAACAA ACTTTTAGTT 
CTGGAAATGC CGCTGGCCCA TAAGATGTCA TCTTTAGTCC TTATCATGCC ATACCACGTA 
GAATCACTGG AAAGAGTGGA GAAACTGCTG ACACGTCAAC AACTGAACAC CTGGGTCAGT 
GCAATGGAAC AGAAAGCAGT AGCAATCTCT CTGCCAAAGG TCAGCATGGA GGTCAGCCAC 
AACCTGCAGA AACATCTCGC TGAGCTGGGT CTGACTGAAG CTGTGGATAA GGCCAAGGCT 
GATCTGTCCA ACATCTCAGG GAAGAAAGAC CTCTATTTGT CCAATGTTTT CCATGCCTCA 
GCCATGGAGT GGGACACAGA GGGAAATCCG CCTGATACTA GCATCTTTGG TACAGACCAG 
CTGAAAAATC CCAAACTCTT CTACGCCGAC CATCCTTTTG TCTTCCTGGT GAAAGACAAC 
AAGACAAACT CTATTCTTTT CATGGGCAGG CTGATCCGGC CGAAGGGTGA TAAAATGAGG 
GATGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional characterization of zebrafish orthologs of the human Beta 3-Glucosyltransferase B3GLCT gene mutated in Peters Plus Syndrome.
PloS one12(9)e0184903(2017)
Weh E,Takeuchi H,Muheisen S,Haltiwanger RS,Semina EV

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Fructose leads to hepatic steatosis in zebrafish that is reversed by mechanistic target of rapamycin (mTOR) inhibition.
Hepatology (Baltimore, Md.)60(5)1581-92(2014 Nov)
Sapp V,Gaffney L,EauClaire SF,Matthews RP

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serpinh1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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