cyb5r4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cyb5r4
Entrez Gene ID	553685
Full Name	cytochrome b5 reductase 4
Synonyms	im:4727699,zgc:112177
General protein information	Preferred Names cytochrome b5 reductase 4 Names cytochrome b5 reductase 4 b5+b5R cb5/cb5R flavohemoprotein b5/b5R ncb5or
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173452.4	XP_005173509.1	cytochrome b5 reductase 4 isoform X1
XM_005173454.3	XP_005173511.1	cytochrome b5 reductase 4 isoform X2
XM_005173454.4	XP_005173511.1	cytochrome b5 reductase 4 isoform X2
XM_005173452.3	XP_005173509.1	cytochrome b5 reductase 4 isoform X1
NM_001020660.1	NP_001018496.1	cytochrome b5 reductase 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CYB5R4	XP_518614.3
Bos taurus (cattle)	CYB5R4	NP_001033248.1
Gallus gallus (chicken)	CYB5R4	XP_001233871.1
Macaca mulatta (Rhesus monkey)	CYB5R4	XP_001085835.1
Canis lupus familiaris (dog)	CYB5R4	XP_532219.3
Mus musculus (house mouse)	Cyb5r4	NP_077157.2
Rattus norvegicus (Norway rat)	Cyb5r4	NP_596918.3
Danio rerio (zebrafish)	cyb5r4	NP_001018496.1
Xenopus tropicalis (tropical clawed frog)	cyb5r4	NP_001016756.1
Drosophila melanogaster (fruit fly)	CG11257	NP_611419.1
Caenorhabditis elegans (roundworm)	CELE_Y52B11A.3	NP_001021784.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011314	XP_309336.4
Homo sapiens (human)	CYB5R4	NP_057314.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish.
Gomez GA, Veldman MB, Zhao Y, Burgess S, Lin S
PloS one4(3)e4994(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cyb5r4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cyb5r4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa21019	XM_005173454.4 Latest version!	Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa37474	NM_001020660.1 Latest version!	Danio rerio cytochrome b5 reductase 4 (cyb5r4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa21017	XM_005173452.4 Latest version!	Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa21019	XM_005173454.3	Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa21017	XM_005173452.3	Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cyb5r4 ( XM_005173454.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173511.1 Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21019
Clone ID Related Accession (Same CDS sequence)	XM_005173454.3 , XM_005173454.4
Accession Version	XM_005173454.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cytochrome b5 reductase 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTGG GCAGGATGTC GTACCTTCTC TACTGGCGCC TGTCCAGTAG AGTTCAAGAT 
CATGTTGATG TGCAGACAGC CCACTCTGTT GGAAAGGTTC AGTTGTGCCT GCGCAAATCC 
GTCAAAGAAA AGTGGACGCA GTTGGGGCAA TCGCTTGAAC ACCATGACAC ATTCATTCAA 
TGTAAAGATC GAGGGCTCTT CTACAGGGAG TGTGTGCTGC TTTCGAAAAC AGACGTCACA 
CACAACACGC AGCTCTTGCG TTTGCAGCTT CCTCCAGGGT CACGTATGCA AGTTCCTGTG 
GGAAGACACG TCTACCTCAA AACGTCTGTC CAGGGTACGG ATGTTGTGAA ACCCTACACA 
GCAGTGGATC AGATGCTAAT ACCTCCCTCA CAGTCCAGTG CTGAAGTGGG CTCAGACATA 
CATCTCATGA TAAAGGTCTA TCCGGATGGA GTGTTGACCC CGCACATCGC CAACCTTCCT 
ATTGGAGCGT CCCTGTCTGT AGGAGGCCCT GAAGGCTCCC TTACTCTGCG AGTCCTACGG 
GATGTCACTC ATCTCTACAT GTTAGCAGCG GGCACAGGAT TCACACCCAT GGCTCGTCTC 
ATCCGGCTGG CCCTGCAGGA ATTCACAGTG ATCAGAAAAA TGAAGCTGAT GTTCTTCAAC 
CGGCAGGAGA GAGACATACT TTGGCAGTCA CAACTGGATG AATTATGTAC AAAAGAGGAA 
AGATTTGAGG TGCAGCATGT TCTCTCAGAG CCTGCAGACT CGTGGACGGG CAGAAGAGGA 
CGGATAGATG CTTGTATGCT GCAGAACTTC CTGGAGAGGC CAGAAAACTC CAAGTGTTTA 
GTGTGCGTGT GCGGCCCTGC TGGATTTACA GAGTCAGCAG TGCAATTGGT CAGACAGCTG 
GACTTCAGTG AAGAGGAACT CCATGTTTTT CAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173511.1
CDS	578..1516
Translation	MLLGRMSYLLYWRLSSRVQDHVDVQTAHSVGKVQLCLRKSVKEKWTQLGQSLEHHDTFIQCKDRGLFYRECVLLSKTDVTHNTQLLRLQLPPGSRMQVPVGRHVYLKTSVQGTDVVKPYTAVDQMLIPPSQSSAEVGSDIHLMIKVYPDGVLTPHIANLPIGASLSVGGPEGSLTLRVLRDVTHLYMLAAGTGFTPMARLIRLALQEFTVIRKMKLMFFNRQERDILWQSQLDELCTKEERFEVQHVLSEPADSWTGRRGRIDACMLQNFLERPENSKCLVCVCGPAGFTESAVQLVRQLDFSEEELHVFQA

Target ORF information:

RefSeq Version	XM_005173454.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173454.3

ORF Insert Sequence:

ATGCTGTTGG GCAGGATGTC GTACCTTCTC TACTGGCGCC TGTCCAGTAG AGTTCAAGAT 
CATGTTGATG TGCAGACAGC CCACTCTGTT GGAAAGGTTC AGTTGTGCCT GCGCAAATCC 
GTCAAAGAAA AGTGGACGCA GTTGGGGCAA TCGCTTGAAC ACCATGACAC ATTCATTCAA 
TGTAAAGATC GAGGGCTCTT CTACAGGGAG TGTGTGCTGC TTTCGAAAAC AGACGTCACA 
CACAACACGC AGCTCTTGCG TTTGCAGCTT CCTCCAGGGT CACGTATGCA AGTTCCTGTG 
GGAAGACACG TCTACCTCAA AACGTCTGTC CAGGGTACGG ATGTTGTGAA ACCCTACACA 
GCAGTGGATC AGATGCTAAT ACCTCCCTCA CAGTCCAGTG CTGAAGTGGG CTCAGACATA 
CATCTCATGA TAAAGGTCTA TCCGGATGGA GTGTTGACCC CGCACATCGC CAACCTTCCT 
ATTGGAGCGT CCCTGTCTGT AGGAGGCCCT GAAGGCTCCC TTACTCTGCG AGTCCTACGG 
GATGTCACTC ATCTCTACAT GTTAGCAGCG GGCACAGGAT TCACACCCAT GGCTCGTCTC 
ATCCGGCTGG CCCTGCAGGA ATTCACAGTG ATCAGAAAAA TGAAGCTGAT GTTCTTCAAC 
CGGCAGGAGA GAGACATACT TTGGCAGTCA CAACTGGATG AATTATGTAC AAAAGAGGAA 
AGATTTGAGG TGCAGCATGT TCTCTCAGAG CCTGCAGACT CGTGGACGGG CAGAAGAGGA 
CGGATAGATG CTTGTATGCT GCAGAACTTC CTGGAGAGGC CAGAAAACTC CAAGTGTTTA 
GTGTGCGTGT GCGGCCCTGC TGGATTTACA GAGTCAGCAG TGCAATTGGT CAGACAGCTG 
GACTTCAGTG AAGAGGAACT CCATGTTTTT CAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cyb5r4 ( XM_005173454.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173511.1 Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21019
Clone ID Related Accession (Same CDS sequence)	XM_005173454.3 , XM_005173454.4
Accession Version	XM_005173454.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cytochrome b5 reductase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173454.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTGG GCAGGATGTC GTACCTTCTC TACTGGCGCC TGTCCAGTAG AGTTCAAGAT 
CATGTTGATG TGCAGACAGC CCACTCTGTT GGAAAGGTTC AGTTGTGCCT GCGCAAATCC 
GTCAAAGAAA AGTGGACGCA GTTGGGGCAA TCGCTTGAAC ACCATGACAC ATTCATTCAA 
TGTAAAGATC GAGGGCTCTT CTACAGGGAG TGTGTGCTGC TTTCGAAAAC AGACGTCACA 
CACAACACGC AGCTCTTGCG TTTGCAGCTT CCTCCAGGGT CACGTATGCA AGTTCCTGTG 
GGAAGACACG TCTACCTCAA AACGTCTGTC CAGGGTACGG ATGTTGTGAA ACCCTACACA 
GCAGTGGATC AGATGCTAAT ACCTCCCTCA CAGTCCAGTG CTGAAGTGGG CTCAGACATA 
CATCTCATGA TAAAGGTCTA TCCGGATGGA GTGTTGACCC CGCACATCGC CAACCTTCCT 
ATTGGAGCGT CCCTGTCTGT AGGAGGCCCT GAAGGCTCCC TTACTCTGCG AGTCCTACGG 
GATGTCACTC ATCTCTACAT GTTAGCAGCG GGCACAGGAT TCACACCCAT GGCTCGTCTC 
ATCCGGCTGG CCCTGCAGGA ATTCACAGTG ATCAGAAAAA TGAAGCTGAT GTTCTTCAAC 
CGGCAGGAGA GAGACATACT TTGGCAGTCA CAACTGGATG AATTATGTAC AAAAGAGGAA 
AGATTTGAGG TGCAGCATGT TCTCTCAGAG CCTGCAGACT CGTGGACGGG CAGAAGAGGA 
CGGATAGATG CTTGTATGCT GCAGAACTTC CTGGAGAGGC CAGAAAACTC CAAGTGTTTA 
GTGTGCGTGT GCGGCCCTGC TGGATTTACA GAGTCAGCAG TGCAATTGGT CAGACAGCTG 
GACTTCAGTG AAGAGGAACT CCATGTTTTT CAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173511.1
CDS	346..1284
Translation	MLLGRMSYLLYWRLSSRVQDHVDVQTAHSVGKVQLCLRKSVKEKWTQLGQSLEHHDTFIQCKDRGLFYRECVLLSKTDVTHNTQLLRLQLPPGSRMQVPVGRHVYLKTSVQGTDVVKPYTAVDQMLIPPSQSSAEVGSDIHLMIKVYPDGVLTPHIANLPIGASLSVGGPEGSLTLRVLRDVTHLYMLAAGTGFTPMARLIRLALQEFTVIRKMKLMFFNRQERDILWQSQLDELCTKEERFEVQHVLSEPADSWTGRRGRIDACMLQNFLERPENSKCLVCVCGPAGFTESAVQLVRQLDFSEEELHVFQA

Target ORF information:

RefSeq Version	XM_005173454.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173454.4

ORF Insert Sequence:

ATGCTGTTGG GCAGGATGTC GTACCTTCTC TACTGGCGCC TGTCCAGTAG AGTTCAAGAT 
CATGTTGATG TGCAGACAGC CCACTCTGTT GGAAAGGTTC AGTTGTGCCT GCGCAAATCC 
GTCAAAGAAA AGTGGACGCA GTTGGGGCAA TCGCTTGAAC ACCATGACAC ATTCATTCAA 
TGTAAAGATC GAGGGCTCTT CTACAGGGAG TGTGTGCTGC TTTCGAAAAC AGACGTCACA 
CACAACACGC AGCTCTTGCG TTTGCAGCTT CCTCCAGGGT CACGTATGCA AGTTCCTGTG 
GGAAGACACG TCTACCTCAA AACGTCTGTC CAGGGTACGG ATGTTGTGAA ACCCTACACA 
GCAGTGGATC AGATGCTAAT ACCTCCCTCA CAGTCCAGTG CTGAAGTGGG CTCAGACATA 
CATCTCATGA TAAAGGTCTA TCCGGATGGA GTGTTGACCC CGCACATCGC CAACCTTCCT 
ATTGGAGCGT CCCTGTCTGT AGGAGGCCCT GAAGGCTCCC TTACTCTGCG AGTCCTACGG 
GATGTCACTC ATCTCTACAT GTTAGCAGCG GGCACAGGAT TCACACCCAT GGCTCGTCTC 
ATCCGGCTGG CCCTGCAGGA ATTCACAGTG ATCAGAAAAA TGAAGCTGAT GTTCTTCAAC 
CGGCAGGAGA GAGACATACT TTGGCAGTCA CAACTGGATG AATTATGTAC AAAAGAGGAA 
AGATTTGAGG TGCAGCATGT TCTCTCAGAG CCTGCAGACT CGTGGACGGG CAGAAGAGGA 
CGGATAGATG CTTGTATGCT GCAGAACTTC CTGGAGAGGC CAGAAAACTC CAAGTGTTTA 
GTGTGCGTGT GCGGCCCTGC TGGATTTACA GAGTCAGCAG TGCAATTGGT CAGACAGCTG 
GACTTCAGTG AAGAGGAACT CCATGTTTTT CAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cyb5r4 ( NM_001020660.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001018496.1 Danio rerio cytochrome b5 reductase 4 (cyb5r4), mRNA.

Price & Availability↑

CloneID	ODa37474
Clone ID Related Accession (Same CDS sequence)	NM_001020660.1
Accession Version	NM_001020660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556553600000
Organism	Danio rerio(zebrafish)
Product	cytochrome b5 reductase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095683.1. ##Evidence-Data-START## Transcript exon combination :: BC095683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAATG TCCCATCTCA GGCTTTCCCT GCTGCTGGAT CCCAGCAGAG AGTCGCGCCA 
GCCGGACAGT CCAGAAACAA GGTGGTGCTG AAGCCTGGTC ACAGTTTGCT GGACTGGATC 
CGACTGACCA AAAGTGGACA GGATTTAACA GGACTGAGAG GTCGACTGAT CGAGGTCACA 
GAAGACGAGC TGAAGAAACA CAACACCAAA AAAGACTGCT GGACGTGCAT TAGAGGTATG 
GTCTATAACT TGAGTGCCTA CATGGATTTC CACCCTGGTG GGGAAGAGGA GTTAATGCGG 
GCTGCTGGGA TTGATAGCAC TGACCTGTTT GACGAGGTCC ACCGATGGGT GAATTATGAG 
TCCATGCTGA AGGAGTGTTT GGTGGGGAGG ATGGCAGTAA AGCCCAGCCC TGCTCTACAA 
GCTCACACAG AGAAGACTGA GAGCACTCAT TTAAACGGTT TATCTGCGCC TCCATCTTTA 
AGACCAGAGC CTTTATCTGC TCCTCTTCCT GCCAAAGACC ATCGTCCTCG GTATGATTGG 
TTCCAGACAG ATGGCACGGT TAATATTGTT GTTTACACCA AGCGAAAGAT TCCCAGCGCC 
GGCTGTGCAG TGGTGGATCT ACAGGATGAT AATCTCAGAG TAGAGATGCT GTTGGGCAGG 
ATGTCGTACC TTCTCTACTG GCGCCTGTCC AGTAGAGTTC AAGATCATGT TGATGTGCAG 
ACAGCCCACT CTGTTGGAAA GGTTCAGTTG TGCCTGCGCA AATCCGTCAA AGAAAAGTGG 
ACGCAGTTGG GGCAATCGCT TGAACACCAT GACACATTCA TTCAATGTAA AGATCGAGGG 
CTCTTCTACA GGGAGTGTGT GCTGCTTTCG AAAACAGACG TCACACACAA CACGCAGCTC 
TTGCGCTTGC AGCTTCCTCG AGGGTCACGT ATGCAAGTTC CTGTGGGAAG ACACGTCTAC 
CTCAAAACGT CTGTCCAGGG TACGGATGTT GTTAAACCCT ACACAGCAGT GGATCAGATG 
CTAATACCTC CCTCACAGTC CAGTGCTGAA GTGGGCTCAG ACATACATCT CATGATAAAG 
GTCTATCCGG ATGGAGTGTT GACCCCGCAC ATCGCCAACC TTCCTATTGG AGCGTCTCTG 
TCTGTAGGAG GCCCTGAAGG CTCCTTTACT CTGCGAGTCC TACGGGATGT CACTCATCTC 
TACATGTTAG CAGCGGGCAC AGGATTCACA CCCATGGCTC GTCTCATCCG GCTGGCCCTG 
CAGGACTTCA CAGTGATCAG AAAAATGAAG CTGATGTTCT TCAACCGGCA GGAGAGAGAC 
ATACTTTGGC AGTCACAACT GGATGAATTA TGTACAAAAG AGGAAAGATT TGAGGTGCAG 
CATGTTCTCT CAGAGCCTGC AGACTCGTGG ACGGGCAGAA GAGGACGGAT AGATGCTTGT 
ATGCTGCAGA ACTTCCTGGA GAGGCCAGAA AACTCCAAGT GTTTAGTGTG CGTGTGCGGC 
CCTGCTGGAT TCACAGAGTC AGCAGTGCAA TTGGTCAGAC AGCTGGACTT CAGTGAAGAG 
GAACTCCATG TTTTTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018496.1
CDS	240..1823
Translation	MLNVPSQAFPAAGSQQRVAPAGQSRNKVVLKPGHSLLDWIRLTKSGQDLTGLRGRLIEVTEDELKKHNTKKDCWTCIRGMVYNLSAYMDFHPGGEEELMRAAGIDSTDLFDEVHRWVNYESMLKECLVGRMAVKPSPALQAHTEKTESTHLNGLSAPPSLRPEPLSAPLPAKDHRPRYDWFQTDGTVNIVVYTKRKIPSAGCAVVDLQDDNLRVEMLLGRMSYLLYWRLSSRVQDHVDVQTAHSVGKVQLCLRKSVKEKWTQLGQSLEHHDTFIQCKDRGLFYRECVLLSKTDVTHNTQLLRLQLPRGSRMQVPVGRHVYLKTSVQGTDVVKPYTAVDQMLIPPSQSSAEVGSDIHLMIKVYPDGVLTPHIANLPIGASLSVGGPEGSFTLRVLRDVTHLYMLAAGTGFTPMARLIRLALQDFTVIRKMKLMFFNRQERDILWQSQLDELCTKEERFEVQHVLSEPADSWTGRRGRIDACMLQNFLERPENSKCLVCVCGPAGFTESAVQLVRQLDFSEEELHVFQA

Target ORF information:

RefSeq Version	NM_001020660.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytochrome b5 reductase 4 (cyb5r4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020660.1

ORF Insert Sequence:

ATGCTGAATG TCCCATCTCA GGCTTTCCCT GCTGCTGGAT CCCAGCAGAG AGTCGCGCCA 
GCCGGACAGT CCAGAAACAA GGTGGTGCTG AAGCCTGGTC ACAGTTTGCT GGACTGGATC 
CGACTGACCA AAAGTGGACA GGATTTAACA GGACTGAGAG GTCGACTGAT CGAGGTCACA 
GAAGACGAGC TGAAGAAACA CAACACCAAA AAAGACTGCT GGACGTGCAT TAGAGGTATG 
GTCTATAACT TGAGTGCCTA CATGGATTTC CACCCTGGTG GGGAAGAGGA GTTAATGCGG 
GCTGCTGGGA TTGATAGCAC TGACCTGTTT GACGAGGTCC ACCGATGGGT GAATTATGAG 
TCCATGCTGA AGGAGTGTTT GGTGGGGAGG ATGGCAGTAA AGCCCAGCCC TGCTCTACAA 
GCTCACACAG AGAAGACTGA GAGCACTCAT TTAAACGGTT TATCTGCGCC TCCATCTTTA 
AGACCAGAGC CTTTATCTGC TCCTCTTCCT GCCAAAGACC ATCGTCCTCG GTATGATTGG 
TTCCAGACAG ATGGCACGGT TAATATTGTT GTTTACACCA AGCGAAAGAT TCCCAGCGCC 
GGCTGTGCAG TGGTGGATCT ACAGGATGAT AATCTCAGAG TAGAGATGCT GTTGGGCAGG 
ATGTCGTACC TTCTCTACTG GCGCCTGTCC AGTAGAGTTC AAGATCATGT TGATGTGCAG 
ACAGCCCACT CTGTTGGAAA GGTTCAGTTG TGCCTGCGCA AATCCGTCAA AGAAAAGTGG 
ACGCAGTTGG GGCAATCGCT TGAACACCAT GACACATTCA TTCAATGTAA AGATCGAGGG 
CTCTTCTACA GGGAGTGTGT GCTGCTTTCG AAAACAGACG TCACACACAA CACGCAGCTC 
TTGCGCTTGC AGCTTCCTCG AGGGTCACGT ATGCAAGTTC CTGTGGGAAG ACACGTCTAC 
CTCAAAACGT CTGTCCAGGG TACGGATGTT GTTAAACCCT ACACAGCAGT GGATCAGATG 
CTAATACCTC CCTCACAGTC CAGTGCTGAA GTGGGCTCAG ACATACATCT CATGATAAAG 
GTCTATCCGG ATGGAGTGTT GACCCCGCAC ATCGCCAACC TTCCTATTGG AGCGTCTCTG 
TCTGTAGGAG GCCCTGAAGG CTCCTTTACT CTGCGAGTCC TACGGGATGT CACTCATCTC 
TACATGTTAG CAGCGGGCAC AGGATTCACA CCCATGGCTC GTCTCATCCG GCTGGCCCTG 
CAGGACTTCA CAGTGATCAG AAAAATGAAG CTGATGTTCT TCAACCGGCA GGAGAGAGAC 
ATACTTTGGC AGTCACAACT GGATGAATTA TGTACAAAAG AGGAAAGATT TGAGGTGCAG 
CATGTTCTCT CAGAGCCTGC AGACTCGTGG ACGGGCAGAA GAGGACGGAT AGATGCTTGT 
ATGCTGCAGA ACTTCCTGGA GAGGCCAGAA AACTCCAAGT GTTTAGTGTG CGTGTGCGGC 
CCTGCTGGAT TCACAGAGTC AGCAGTGCAA TTGGTCAGAC AGCTGGACTT CAGTGAAGAG 
GAACTCCATG TTTTTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cyb5r4 ( XM_005173452.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173509.1 Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21017
Clone ID Related Accession (Same CDS sequence)	XM_005173452.4 , XM_005173452.3
Accession Version	XM_005173452.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cytochrome b5 reductase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173452.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGAAG AAGATGGTAT GGACTTGGAG GAGGAAGAAG AGGAAGATTA TAGATCAGAA 
AGCAGAAGGA AAGCTTCAAC AGTCTTTTCT GTCATCGCCA GTGTTTTGTC TGGATTTTTA 
GGGAACTGGG CTTGTTTTAA AGTATCAGAG ATGCTGAATG TCCCATCTCA GGCTTTCCCT 
GCTGCTGGAT CCCAGCAGAG AGTCGCGCCA GCCGGACAGT CCAGAAACAA GGTGGTGCTG 
AAGCCTGGTC ACAGTTTGCT GGACTGGATC CGACTGACCA AAAGTGGACA GGATTTAACA 
GGACTGAGAG GTCGACTGAT CGAGGTCACA GAAGACGAGC TGAAGAAACA CAACACCAAA 
AAAGACTGCT GGACGTGCAT TAGAGGTATG GTCTATAACT TGAGTGCCTA CATGGATTTC 
CACCCTGGTG GGGAAGAGGA GTTAATGCGG GCTGCTGGGA TTGATAGCAC TGACCTGTTT 
GACGAGGTCC ACCGATGGGT GAATTATGAG TCCATGCTGA AGGAGTGTTT GGTGGGGAGG 
ATGGCAGTAA AGCCCAGCCC TGCTCTACAA GCTCACACAG AGAAGACTGA GAGCACTCAT 
TTAAACGGTT TATCTGCGCC TCCATCTTTA AGACCAGAGC CTTTATCTGC TCCTCTTCCT 
GCCAAAGACC ATCGTCCTCG GTATGATTGG TTCCAGACAG ATGGCACTGT TAATATTGTT 
GTTTACACCA AGCGAAAGAT TCCCAGCGCC GGCTGTGCAG TGGTGGATCT ACAGGATGAT 
AATCTCAGAG TAGAGATGCT GTTGGGCAGG ATGTCGTACC TTCTCTACTG GCGCCTGTCC 
AGTAGAGTTC AAGATCATGT TGATGTGCAG ACAGCCCACT CTGTTGGAAA GGTTCAGTTG 
TGCCTGCGCA AATCCGTCAA AGAAAAGTGG ACGCAGTTGG GGCAATCGCT TGAACACCAT 
GACACATTCA TTCAATGTAA AGATCGAGGG CTCTTCTACA GGGAGTGTGT GCTGCTTTCG 
AAAACAGACG TCACACACAA CACGCAGCTC TTGCGTTTGC AGCTTCCTCC AGGGTCACGT 
ATGCAAGTTC CTGTGGGAAG ACACGTCTAC CTCAAAACGT CTGTCCAGGG TACGGATGTT 
GTGAAACCCT ACACAGCAGT GGATCAGATG CTAATACCTC CCTCACAGTC CAGTGCTGAA 
GTGGGCTCAG ACATACATCT CATGATAAAG GTCTATCCGG ATGGAGTGTT GACCCCGCAC 
ATCGCCAACC TTCCTATTGG AGCGTCCCTG TCTGTAGGAG GCCCTGAAGG CTCCCTTACT 
CTGCGAGTCC TACGGGATGT CACTCATCTC TACATGTTAG CAGCGGGCAC AGGATTCACA 
CCCATGGCTC GTCTCATCCG GCTGGCCCTG CAGGAATTCA CAGTGATCAG AAAAATGAAG 
CTGATGTTCT TCAACCGGCA GGAGAGAGAC ATACTTTGGC AGTCACAACT GGATGAATTA 
TGTACAAAAG AGGAAAGATT TGAGGTGCAG CATGTTCTCT CAGAGCCTGC AGACTCGTGG 
ACGGGCAGAA GAGGACGGAT AGATGCTTGT ATGCTGCAGA ACTTCCTGGA GAGGCCAGAA 
AACTCCAAGT GTTTAGTGTG CGTGTGCGGC CCTGCTGGAT TTACAGAGTC AGCAGTGCAA 
TTGGTCAGAC AGCTGGACTT CAGTGAAGAG GAACTCCATG TTTTTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173509.1
CDS	165..1898
Translation	MVEEDGMDLEEEEEEDYRSESRRKASTVFSVIASVLSGFLGNWACFKVSEMLNVPSQAFPAAGSQQRVAPAGQSRNKVVLKPGHSLLDWIRLTKSGQDLTGLRGRLIEVTEDELKKHNTKKDCWTCIRGMVYNLSAYMDFHPGGEEELMRAAGIDSTDLFDEVHRWVNYESMLKECLVGRMAVKPSPALQAHTEKTESTHLNGLSAPPSLRPEPLSAPLPAKDHRPRYDWFQTDGTVNIVVYTKRKIPSAGCAVVDLQDDNLRVEMLLGRMSYLLYWRLSSRVQDHVDVQTAHSVGKVQLCLRKSVKEKWTQLGQSLEHHDTFIQCKDRGLFYRECVLLSKTDVTHNTQLLRLQLPPGSRMQVPVGRHVYLKTSVQGTDVVKPYTAVDQMLIPPSQSSAEVGSDIHLMIKVYPDGVLTPHIANLPIGASLSVGGPEGSLTLRVLRDVTHLYMLAAGTGFTPMARLIRLALQEFTVIRKMKLMFFNRQERDILWQSQLDELCTKEERFEVQHVLSEPADSWTGRRGRIDACMLQNFLERPENSKCLVCVCGPAGFTESAVQLVRQLDFSEEELHVFQA

Target ORF information:

RefSeq Version	XM_005173452.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173452.4

ORF Insert Sequence:

ATGGTAGAAG AAGATGGTAT GGACTTGGAG GAGGAAGAAG AGGAAGATTA TAGATCAGAA 
AGCAGAAGGA AAGCTTCAAC AGTCTTTTCT GTCATCGCCA GTGTTTTGTC TGGATTTTTA 
GGGAACTGGG CTTGTTTTAA AGTATCAGAG ATGCTGAATG TCCCATCTCA GGCTTTCCCT 
GCTGCTGGAT CCCAGCAGAG AGTCGCGCCA GCCGGACAGT CCAGAAACAA GGTGGTGCTG 
AAGCCTGGTC ACAGTTTGCT GGACTGGATC CGACTGACCA AAAGTGGACA GGATTTAACA 
GGACTGAGAG GTCGACTGAT CGAGGTCACA GAAGACGAGC TGAAGAAACA CAACACCAAA 
AAAGACTGCT GGACGTGCAT TAGAGGTATG GTCTATAACT TGAGTGCCTA CATGGATTTC 
CACCCTGGTG GGGAAGAGGA GTTAATGCGG GCTGCTGGGA TTGATAGCAC TGACCTGTTT 
GACGAGGTCC ACCGATGGGT GAATTATGAG TCCATGCTGA AGGAGTGTTT GGTGGGGAGG 
ATGGCAGTAA AGCCCAGCCC TGCTCTACAA GCTCACACAG AGAAGACTGA GAGCACTCAT 
TTAAACGGTT TATCTGCGCC TCCATCTTTA AGACCAGAGC CTTTATCTGC TCCTCTTCCT 
GCCAAAGACC ATCGTCCTCG GTATGATTGG TTCCAGACAG ATGGCACTGT TAATATTGTT 
GTTTACACCA AGCGAAAGAT TCCCAGCGCC GGCTGTGCAG TGGTGGATCT ACAGGATGAT 
AATCTCAGAG TAGAGATGCT GTTGGGCAGG ATGTCGTACC TTCTCTACTG GCGCCTGTCC 
AGTAGAGTTC AAGATCATGT TGATGTGCAG ACAGCCCACT CTGTTGGAAA GGTTCAGTTG 
TGCCTGCGCA AATCCGTCAA AGAAAAGTGG ACGCAGTTGG GGCAATCGCT TGAACACCAT 
GACACATTCA TTCAATGTAA AGATCGAGGG CTCTTCTACA GGGAGTGTGT GCTGCTTTCG 
AAAACAGACG TCACACACAA CACGCAGCTC TTGCGTTTGC AGCTTCCTCC AGGGTCACGT 
ATGCAAGTTC CTGTGGGAAG ACACGTCTAC CTCAAAACGT CTGTCCAGGG TACGGATGTT 
GTGAAACCCT ACACAGCAGT GGATCAGATG CTAATACCTC CCTCACAGTC CAGTGCTGAA 
GTGGGCTCAG ACATACATCT CATGATAAAG GTCTATCCGG ATGGAGTGTT GACCCCGCAC 
ATCGCCAACC TTCCTATTGG AGCGTCCCTG TCTGTAGGAG GCCCTGAAGG CTCCCTTACT 
CTGCGAGTCC TACGGGATGT CACTCATCTC TACATGTTAG CAGCGGGCAC AGGATTCACA 
CCCATGGCTC GTCTCATCCG GCTGGCCCTG CAGGAATTCA CAGTGATCAG AAAAATGAAG 
CTGATGTTCT TCAACCGGCA GGAGAGAGAC ATACTTTGGC AGTCACAACT GGATGAATTA 
TGTACAAAAG AGGAAAGATT TGAGGTGCAG CATGTTCTCT CAGAGCCTGC AGACTCGTGG 
ACGGGCAGAA GAGGACGGAT AGATGCTTGT ATGCTGCAGA ACTTCCTGGA GAGGCCAGAA 
AACTCCAAGT GTTTAGTGTG CGTGTGCGGC CCTGCTGGAT TTACAGAGTC AGCAGTGCAA 
TTGGTCAGAC AGCTGGACTT CAGTGAAGAG GAACTCCATG TTTTTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cyb5r4 ( XM_005173452.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173509.1 Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X1, mRNA.

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CloneID	ODa21017
Clone ID Related Accession (Same CDS sequence)	XM_005173452.4 , XM_005173452.3
Accession Version	XM_005173452.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cytochrome b5 reductase 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGAAG AAGATGGTAT GGACTTGGAG GAGGAAGAAG AGGAAGATTA TAGATCAGAA 
AGCAGAAGGA AAGCTTCAAC AGTCTTTTCT GTCATCGCCA GTGTTTTGTC TGGATTTTTA 
GGGAACTGGG CTTGTTTTAA AGTATCAGAG ATGCTGAATG TCCCATCTCA GGCTTTCCCT 
GCTGCTGGAT CCCAGCAGAG AGTCGCGCCA GCCGGACAGT CCAGAAACAA GGTGGTGCTG 
AAGCCTGGTC ACAGTTTGCT GGACTGGATC CGACTGACCA AAAGTGGACA GGATTTAACA 
GGACTGAGAG GTCGACTGAT CGAGGTCACA GAAGACGAGC TGAAGAAACA CAACACCAAA 
AAAGACTGCT GGACGTGCAT TAGAGGTATG GTCTATAACT TGAGTGCCTA CATGGATTTC 
CACCCTGGTG GGGAAGAGGA GTTAATGCGG GCTGCTGGGA TTGATAGCAC TGACCTGTTT 
GACGAGGTCC ACCGATGGGT GAATTATGAG TCCATGCTGA AGGAGTGTTT GGTGGGGAGG 
ATGGCAGTAA AGCCCAGCCC TGCTCTACAA GCTCACACAG AGAAGACTGA GAGCACTCAT 
TTAAACGGTT TATCTGCGCC TCCATCTTTA AGACCAGAGC CTTTATCTGC TCCTCTTCCT 
GCCAAAGACC ATCGTCCTCG GTATGATTGG TTCCAGACAG ATGGCACTGT TAATATTGTT 
GTTTACACCA AGCGAAAGAT TCCCAGCGCC GGCTGTGCAG TGGTGGATCT ACAGGATGAT 
AATCTCAGAG TAGAGATGCT GTTGGGCAGG ATGTCGTACC TTCTCTACTG GCGCCTGTCC 
AGTAGAGTTC AAGATCATGT TGATGTGCAG ACAGCCCACT CTGTTGGAAA GGTTCAGTTG 
TGCCTGCGCA AATCCGTCAA AGAAAAGTGG ACGCAGTTGG GGCAATCGCT TGAACACCAT 
GACACATTCA TTCAATGTAA AGATCGAGGG CTCTTCTACA GGGAGTGTGT GCTGCTTTCG 
AAAACAGACG TCACACACAA CACGCAGCTC TTGCGTTTGC AGCTTCCTCC AGGGTCACGT 
ATGCAAGTTC CTGTGGGAAG ACACGTCTAC CTCAAAACGT CTGTCCAGGG TACGGATGTT 
GTGAAACCCT ACACAGCAGT GGATCAGATG CTAATACCTC CCTCACAGTC CAGTGCTGAA 
GTGGGCTCAG ACATACATCT CATGATAAAG GTCTATCCGG ATGGAGTGTT GACCCCGCAC 
ATCGCCAACC TTCCTATTGG AGCGTCCCTG TCTGTAGGAG GCCCTGAAGG CTCCCTTACT 
CTGCGAGTCC TACGGGATGT CACTCATCTC TACATGTTAG CAGCGGGCAC AGGATTCACA 
CCCATGGCTC GTCTCATCCG GCTGGCCCTG CAGGAATTCA CAGTGATCAG AAAAATGAAG 
CTGATGTTCT TCAACCGGCA GGAGAGAGAC ATACTTTGGC AGTCACAACT GGATGAATTA 
TGTACAAAAG AGGAAAGATT TGAGGTGCAG CATGTTCTCT CAGAGCCTGC AGACTCGTGG 
ACGGGCAGAA GAGGACGGAT AGATGCTTGT ATGCTGCAGA ACTTCCTGGA GAGGCCAGAA 
AACTCCAAGT GTTTAGTGTG CGTGTGCGGC CCTGCTGGAT TTACAGAGTC AGCAGTGCAA 
TTGGTCAGAC AGCTGGACTT CAGTGAAGAG GAACTCCATG TTTTTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173509.1
CDS	151..1884
Translation	MVEEDGMDLEEEEEEDYRSESRRKASTVFSVIASVLSGFLGNWACFKVSEMLNVPSQAFPAAGSQQRVAPAGQSRNKVVLKPGHSLLDWIRLTKSGQDLTGLRGRLIEVTEDELKKHNTKKDCWTCIRGMVYNLSAYMDFHPGGEEELMRAAGIDSTDLFDEVHRWVNYESMLKECLVGRMAVKPSPALQAHTEKTESTHLNGLSAPPSLRPEPLSAPLPAKDHRPRYDWFQTDGTVNIVVYTKRKIPSAGCAVVDLQDDNLRVEMLLGRMSYLLYWRLSSRVQDHVDVQTAHSVGKVQLCLRKSVKEKWTQLGQSLEHHDTFIQCKDRGLFYRECVLLSKTDVTHNTQLLRLQLPPGSRMQVPVGRHVYLKTSVQGTDVVKPYTAVDQMLIPPSQSSAEVGSDIHLMIKVYPDGVLTPHIANLPIGASLSVGGPEGSLTLRVLRDVTHLYMLAAGTGFTPMARLIRLALQEFTVIRKMKLMFFNRQERDILWQSQLDELCTKEERFEVQHVLSEPADSWTGRRGRIDACMLQNFLERPENSKCLVCVCGPAGFTESAVQLVRQLDFSEEELHVFQA

Target ORF information:

RefSeq Version	XM_005173452.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cytochrome b5 reductase 4 (cyb5r4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173452.3

ORF Insert Sequence:

ATGGTAGAAG AAGATGGTAT GGACTTGGAG GAGGAAGAAG AGGAAGATTA TAGATCAGAA 
AGCAGAAGGA AAGCTTCAAC AGTCTTTTCT GTCATCGCCA GTGTTTTGTC TGGATTTTTA 
GGGAACTGGG CTTGTTTTAA AGTATCAGAG ATGCTGAATG TCCCATCTCA GGCTTTCCCT 
GCTGCTGGAT CCCAGCAGAG AGTCGCGCCA GCCGGACAGT CCAGAAACAA GGTGGTGCTG 
AAGCCTGGTC ACAGTTTGCT GGACTGGATC CGACTGACCA AAAGTGGACA GGATTTAACA 
GGACTGAGAG GTCGACTGAT CGAGGTCACA GAAGACGAGC TGAAGAAACA CAACACCAAA 
AAAGACTGCT GGACGTGCAT TAGAGGTATG GTCTATAACT TGAGTGCCTA CATGGATTTC 
CACCCTGGTG GGGAAGAGGA GTTAATGCGG GCTGCTGGGA TTGATAGCAC TGACCTGTTT 
GACGAGGTCC ACCGATGGGT GAATTATGAG TCCATGCTGA AGGAGTGTTT GGTGGGGAGG 
ATGGCAGTAA AGCCCAGCCC TGCTCTACAA GCTCACACAG AGAAGACTGA GAGCACTCAT 
TTAAACGGTT TATCTGCGCC TCCATCTTTA AGACCAGAGC CTTTATCTGC TCCTCTTCCT 
GCCAAAGACC ATCGTCCTCG GTATGATTGG TTCCAGACAG ATGGCACTGT TAATATTGTT 
GTTTACACCA AGCGAAAGAT TCCCAGCGCC GGCTGTGCAG TGGTGGATCT ACAGGATGAT 
AATCTCAGAG TAGAGATGCT GTTGGGCAGG ATGTCGTACC TTCTCTACTG GCGCCTGTCC 
AGTAGAGTTC AAGATCATGT TGATGTGCAG ACAGCCCACT CTGTTGGAAA GGTTCAGTTG 
TGCCTGCGCA AATCCGTCAA AGAAAAGTGG ACGCAGTTGG GGCAATCGCT TGAACACCAT 
GACACATTCA TTCAATGTAA AGATCGAGGG CTCTTCTACA GGGAGTGTGT GCTGCTTTCG 
AAAACAGACG TCACACACAA CACGCAGCTC TTGCGTTTGC AGCTTCCTCC AGGGTCACGT 
ATGCAAGTTC CTGTGGGAAG ACACGTCTAC CTCAAAACGT CTGTCCAGGG TACGGATGTT 
GTGAAACCCT ACACAGCAGT GGATCAGATG CTAATACCTC CCTCACAGTC CAGTGCTGAA 
GTGGGCTCAG ACATACATCT CATGATAAAG GTCTATCCGG ATGGAGTGTT GACCCCGCAC 
ATCGCCAACC TTCCTATTGG AGCGTCCCTG TCTGTAGGAG GCCCTGAAGG CTCCCTTACT 
CTGCGAGTCC TACGGGATGT CACTCATCTC TACATGTTAG CAGCGGGCAC AGGATTCACA 
CCCATGGCTC GTCTCATCCG GCTGGCCCTG CAGGAATTCA CAGTGATCAG AAAAATGAAG 
CTGATGTTCT TCAACCGGCA GGAGAGAGAC ATACTTTGGC AGTCACAACT GGATGAATTA 
TGTACAAAAG AGGAAAGATT TGAGGTGCAG CATGTTCTCT CAGAGCCTGC AGACTCGTGG 
ACGGGCAGAA GAGGACGGAT AGATGCTTGT ATGCTGCAGA ACTTCCTGGA GAGGCCAGAA 
AACTCCAAGT GTTTAGTGTG CGTGTGCGGC CCTGCTGGAT TTACAGAGTC AGCAGTGCAA 
TTGGTCAGAC AGCTGGACTT CAGTGAAGAG GAACTCCATG TTTTTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish.
PloS one4(3)e4994(2009)
Gomez GA,Veldman MB,Zhao Y,Burgess S,Lin S

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cyb5r4 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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