Cyb5r4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyb5r4
Entrez Gene ID	171015
Full Name	cytochrome b5 reductase 4
Synonyms	Ncb5or,b5&b5R,b5+b5R,cb5/cb5R
General protein information	Preferred Names cytochrome b5 reductase 4 Names cytochrome b5 reductase 4 N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein NADPH cytochrome B5 oxidoreductase flavohemoprotein b5+b5R flavohemoprotein b5/b5R
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243472.3	XP_006243534.1	cytochrome b5 reductase 4 isoform X1
NM_133427.3	NP_596918.3	cytochrome b5 reductase 4
XM_017595428.1	XP_017450917.1	cytochrome b5 reductase 4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CYB5R4	XP_518614.3
Bos taurus (cattle)	CYB5R4	NP_001033248.1
Gallus gallus (chicken)	CYB5R4	XP_001233871.1
Macaca mulatta (Rhesus monkey)	CYB5R4	XP_001085835.1
Canis lupus familiaris (dog)	CYB5R4	XP_532219.3
Mus musculus (house mouse)	Cyb5r4	NP_077157.2
Rattus norvegicus (Norway rat)	Cyb5r4	NP_596918.3
Danio rerio (zebrafish)	cyb5r4	NP_001018496.1
Xenopus tropicalis (tropical clawed frog)	cyb5r4	NP_001016756.1
Drosophila melanogaster (fruit fly)	CG11257	NP_611419.1
Caenorhabditis elegans (roundworm)	CELE_Y52B11A.3	NP_001021784.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011314	XP_309336.4
Homo sapiens (human)	CYB5R4	NP_057314.2

Gene Ontology
Bibliography

Related articles in PubMed

The reductase NCB5OR is responsive to the redox status in beta-cells and is not involved in the ER stress response.
Larade K, Jiang ZG, Dejam A, Zhu H, Bunn HF
The Biochemical journal404(3)467-76(2007 Jun)

Mutagenesis of Glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase.
Roma GW, Crowley LJ, Davis CA, Barber MJ
Biochemistry44(41)13467-76(2005 Oct)

Variation in NCB5OR: studies of relationships to type 2 diabetes, maturity-onset diabetes of the young, and gestational diabetes mellitus.
Andersen G, Wegner L, Rose CS, Xie J, Zhu H, Larade K, Johansen A, Ek J, Lauenborg J, Drivsholm T, Borch-Johnsen K, Damm P, Hansen T, Bunn HF, Pedersen O
Diabetes53(11)2992-7(2004 Nov)

NCB5OR is a novel soluble NAD(P)H reductase localized in the endoplasmic reticulum.
Zhu H, Larade K, Jackson TA, Xie J, Ladoux A, Acker H, Berchner-Pfannschmidt U, Fandrey J, Cross AR, Lukat-Rodgers GS, Rodgers KR, Bunn HF
The Journal of biological chemistry279(29)30316-25(2004 Jul)

Absence of a reductase, NCB5OR, causes insulin-deficient diabetes.
Xie J, Zhu H, Larade K, Ladoux A, Seguritan A, Chu M, Ito S, Bronson RT, Leiter EH, Zhang CY, Rosen ED, Bunn HF
Proceedings of the National Academy of Sciences of the United States of America101(29)10750-5(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cyb5r4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyb5r4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa62285	XM_017595428.1 Latest version!	Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa00775	NM_133427.3 Latest version!	Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa31416	XM_006243472.3 Latest version!	Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyb5r4 ( XM_017595428.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450917.1 Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62285
Clone ID Related Accession (Same CDS sequence)	XM_017595428.1
Accession Version	XM_017595428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome b5 reductase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTTGCCTCC 
CAGGGGCGTA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGATTG 
ACCAAAAGTG GAAAGGACTT CACGGGATTG AAGGGCGGGT TAATTGAAGT AACAGAAGAA 
GAACTTAAGA AGCACAACAA GAAAGACGAC TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AATGTCAGCC CATATATGGA GTATCATCCT GGTGGGGAGG ATGAGCTTAT GAGAGCCGCA 
GGAGCAGATG GAACGGATCT TTTTAATGAG GTGCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAGT GCCTGGTTGG CAGGATGGCT GTGAAGCCCG CTGTTCCGAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAGTGACAGA CACACTGCCC 
AGGGAAGGCC CCAGTTCTCC AAGCTATGAT TGGTTCCAAA CAGAGTCTTC AGTGACCATT 
GTTATATACA CCAAGCAGAA GAATATCAAT TTAGACTCAG TAATCGTTGA CCTCCAGGAT 
GACTCCTTGA GAGCAGAAGC AGTCATCAAG GATCATTCTT ATCTTATACA TATTGGGTTA 
AGCCATGAAG TTCAAGAAAA TTTTTCTGTA CGGGTTATTG AGAATGTTGG AAAAATAGAG 
ATTGTCCTAC AAAAGAAAGA AACTGTTTCT TGGAAATGTC TTGGTGATCC CCTGGAGAAA 
CATGATTCTT TTATTCCAAA GAAAGATACA GGCTTGTACT ATAGACAGTG CCAGTTAATT 
TCCAAGGAAG ACGTTACTCA TGACACAAGG CTCTTCTGTC TGATGCTGCC TCCCAGTACT 
CACCTTCAGG TGCCAGTTGG GCAACATGTT TACCTCAAGC TCAGTGTCAC AGGAGATTTT 
GTTTCTGTAA GTGGTCCTGA GGGCAATTTT AAAGTTTCCA AACTCCAGGA AGTGGAAGAT 
CTCTTTTTAT TGGCAGCCGG AACAGGCTTC ACACCAATGG TTACTGTACT GAATCACGCT 
TTAACTCACA TGTCCAGTCT CAGGAAGGTG AAGTTAATGT TCTTCAATAA AACAGAGGAT 
GACATAATTT GGAGATGCCA GCTAGAGAAA TTGGCATTAA AAGATAAAAG ATTTCATGTT 
GAATATGTTC TCTCGGCACC TTCCCCAGAA TGGAATGGCA AACAGGGACA CGTGTCACGA 
GCTCTTCTCT CTGAATTTTT GCAACGAAGT TTAGAAAATT CTAAAGTCTT CCTTTGCATC 
TGTGGACCAA CCCCATTTAC AGATGAGGGG ATAAGGTTGC TGCATGATCT TAACTTTTCC 
GATGATGAGA TTCATGGTTT TACAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450917.1
CDS	111..1520
Translation	MLNVPSQAFPAPGSQQRVASQGRSKVPLKQGRSLMDWIRLTKSGKDFTGLKGGLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQVTDTLPREGPSSPSYDWFQTESSVTIVIYTKQKNINLDSVIVDLQDDSLRAEAVIKDHSYLIHIGLSHEVQENFSVRVIENVGKIEIVLQKKETVSWKCLGDPLEKHDSFIPKKDTGLYYRQCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGDFVSVSGPEGNFKVSKLQEVEDLFLLAAGTGFTPMVTVLNHALTHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALKDKRFHVEYVLSAPSPEWNGKQGHVSRALLSEFLQRSLENSKVFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	XM_017595428.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595428.1

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTTGCCTCC 
CAGGGGCGTA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGATTG 
ACCAAAAGTG GAAAGGACTT CACGGGATTG AAGGGCGGGT TAATTGAAGT AACAGAAGAA 
GAACTTAAGA AGCACAACAA GAAAGACGAC TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AATGTCAGCC CATATATGGA GTATCATCCT GGTGGGGAGG ATGAGCTTAT GAGAGCCGCA 
GGAGCAGATG GAACGGATCT TTTTAATGAG GTGCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAGT GCCTGGTTGG CAGGATGGCT GTGAAGCCCG CTGTTCCGAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAGTGACAGA CACACTGCCC 
AGGGAAGGCC CCAGTTCTCC AAGCTATGAT TGGTTCCAAA CAGAGTCTTC AGTGACCATT 
GTTATATACA CCAAGCAGAA GAATATCAAT TTAGACTCAG TAATCGTTGA CCTCCAGGAT 
GACTCCTTGA GAGCAGAAGC AGTCATCAAG GATCATTCTT ATCTTATACA TATTGGGTTA 
AGCCATGAAG TTCAAGAAAA TTTTTCTGTA CGGGTTATTG AGAATGTTGG AAAAATAGAG 
ATTGTCCTAC AAAAGAAAGA AACTGTTTCT TGGAAATGTC TTGGTGATCC CCTGGAGAAA 
CATGATTCTT TTATTCCAAA GAAAGATACA GGCTTGTACT ATAGACAGTG CCAGTTAATT 
TCCAAGGAAG ACGTTACTCA TGACACAAGG CTCTTCTGTC TGATGCTGCC TCCCAGTACT 
CACCTTCAGG TGCCAGTTGG GCAACATGTT TACCTCAAGC TCAGTGTCAC AGGAGATTTT 
GTTTCTGTAA GTGGTCCTGA GGGCAATTTT AAAGTTTCCA AACTCCAGGA AGTGGAAGAT 
CTCTTTTTAT TGGCAGCCGG AACAGGCTTC ACACCAATGG TTACTGTACT GAATCACGCT 
TTAACTCACA TGTCCAGTCT CAGGAAGGTG AAGTTAATGT TCTTCAATAA AACAGAGGAT 
GACATAATTT GGAGATGCCA GCTAGAGAAA TTGGCATTAA AAGATAAAAG ATTTCATGTT 
GAATATGTTC TCTCGGCACC TTCCCCAGAA TGGAATGGCA AACAGGGACA CGTGTCACGA 
GCTCTTCTCT CTGAATTTTT GCAACGAAGT TTAGAAAATT CTAAAGTCTT CCTTTGCATC 
TGTGGACCAA CCCCATTTAC AGATGAGGGG ATAAGGTTGC TGCATGATCT TAACTTTTCC 
GATGATGAGA TTCATGGTTT TACAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyb5r4 ( NM_133427.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596918.3 Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), mRNA.

Price & Availability↑

CloneID	ORa00775
Clone ID Related Accession (Same CDS sequence)	NM_133427.3
Accession Version	NM_133427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559750400000
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome b5 reductase 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117045.6 and BC080240.1. On Sep 11, 2012 this sequence version replaced NM_133427.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC080240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTTGCCTCC 
CAGGGGCGTA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGATTG 
ACCAAAAGTG GAAAGGACTT CACGGGATTG AAGGGCGGGT TAATTGAAGT AACAGAAGAA 
GAACTTAAGA AGCACAACAA GAAAGACGAC TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AATGTCAGCC CATATATGGA GTATCATCCT GGTGGGGAGG ATGAGCTTAT GAGAGCCGCA 
GGAGCAGATG GAACGGATCT TTTTAATGAG GTGCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAGT GCCTGGTTGG CAGGATGGCT GTGAAGCCCG CTGTTCCGAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAGTGACAGA CACACTGCCC 
AGGGAAGGCC CCAGTTCTCC AAGCTATGAT TGGTTCCAAA CAGAGTCTTC AGTGACCATT 
GTTATATACA CCAAGCAGAA GAATATCAAT TTAGACTCAG TAATCGTTGA CCTCCAGGAT 
GACTCCTTGA GAGCAGAAGC AGTCATCAAG GATCATTCTT ATCTTATACA TATTGGGTTA 
AGCCATGAAG TTCAAGAAAA TTTTTCTGTA CGGGTTATTG AGAATGTTGG AAAAATAGAG 
ATTGTCCTAC AAAAGAAAGA AACTGTTTCT TGGAAATGTC TTGGTGATCC CCTGGAGAAA 
CATGATTCTT TTATTCCAAA GAAAGATACA GGCTTGTACT ATAGACAGTG CCAGTTAATT 
TCCAAGGAAG ACGTTACTCA TGACACAAGG CTCTTCTGTC TGATGCTGCC TCCCAGTACT 
CACCTTCAGG TGCCAGTTGG GCAACATGTT TACCTCAAGC TCAGTGTCAC AGGTGCAGAA 
ATAGTAAAGC CATATACACC CGTGTCTGAG TCCTTACTCT CAGATTTTAA AGAACCAGTT 
CTTTCCCCAA ATAAGTACAT CTACTTTTTG ATCAAAATCT ATCCTGCTGG ACTCTTCACG 
CCAGAACTTG ATCGTCTTCA GATTGGAGAT TTTGTTTCTG TAAGTGGTCC TGAGGGCAAT 
TTTAAAGTTT CCAAACTCCA GGAAGTGGAA GATCTCTTTT TATTGGCAGC CGGAACAGGC 
TTCACACCAA TGGTTACTGT ACTGAATCAC GCTTTAACTC ACATGTCCAG TCTCAGGAAG 
GTGAAGTTAA TGTTCTTCAA TAAAACAGAG GATGACATAA TTTGGAGATG CCAGCTAGAG 
AAATTGGCAT TAAAAGATAA AAGATTTCAT GTTGAATATG TTCTCTCGGC ACCTTCCCCA 
GAATGGAATG GCAAACAGGG ACACGTGTCA CGAGCTCTTC TCTCTGAATT TTTGCAACGA 
AGTTTAGAAA ATTCTAAAGT CTTCCTTTGC ATCTGTGGAC CAACCCCATT TACAGATGAG 
GGGATAAGGT TGCTGCATGA TCTTAACTTT TCCGATGATG AGATTCATGG TTTTACAGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596918.3
CDS	140..1702
Translation	MLNVPSQAFPAPGSQQRVASQGRSKVPLKQGRSLMDWIRLTKSGKDFTGLKGGLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQVTDTLPREGPSSPSYDWFQTESSVTIVIYTKQKNINLDSVIVDLQDDSLRAEAVIKDHSYLIHIGLSHEVQENFSVRVIENVGKIEIVLQKKETVSWKCLGDPLEKHDSFIPKKDTGLYYRQCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSESLLSDFKEPVLSPNKYIYFLIKIYPAGLFTPELDRLQIGDFVSVSGPEGNFKVSKLQEVEDLFLLAAGTGFTPMVTVLNHALTHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALKDKRFHVEYVLSAPSPEWNGKQGHVSRALLSEFLQRSLENSKVFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	NM_133427.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133427.3

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTTGCCTCC 
CAGGGGCGTA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGATTG 
ACCAAAAGTG GAAAGGACTT CACGGGATTG AAGGGCGGGT TAATTGAAGT AACAGAAGAA 
GAACTTAAGA AGCACAACAA GAAAGACGAC TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AATGTCAGCC CATATATGGA GTATCATCCT GGTGGGGAGG ATGAGCTTAT GAGAGCCGCA 
GGAGCAGATG GAACGGATCT TTTTAATGAG GTGCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAGT GCCTGGTTGG CAGGATGGCT GTGAAGCCCG CTGTTCCGAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAGTGACAGA CACACTGCCC 
AGGGAAGGCC CCAGTTCTCC AAGCTATGAT TGGTTCCAAA CAGAGTCTTC AGTGACCATT 
GTTATATACA CCAAGCAGAA GAATATCAAT TTAGACTCAG TAATCGTTGA CCTCCAGGAT 
GACTCCTTGA GAGCAGAAGC AGTCATCAAG GATCATTCTT ATCTTATACA TATTGGGTTA 
AGCCATGAAG TTCAAGAAAA TTTTTCTGTA CGGGTTATTG AGAATGTTGG AAAAATAGAG 
ATTGTCCTAC AAAAGAAAGA AACTGTTTCT TGGAAATGTC TTGGTGATCC CCTGGAGAAA 
CATGATTCTT TTATTCCAAA GAAAGATACA GGCTTGTACT ATAGACAGTG CCAGTTAATT 
TCCAAGGAAG ACGTTACTCA TGACACAAGG CTCTTCTGTC TGATGCTGCC TCCCAGTACT 
CACCTTCAGG TGCCAGTTGG GCAACATGTT TACCTCAAGC TCAGTGTCAC AGGTGCAGAA 
ATAGTAAAGC CATATACACC CGTGTCTGAG TCCTTACTCT CAGATTTTAA AGAACCAGTT 
CTTTCCCCAA ATAAGTACAT CTACTTTTTG ATCAAAATCT ATCCTGCTGG ACTCTTCACG 
CCAGAACTTG ATCGTCTTCA GATTGGAGAT TTTGTTTCTG TAAGTGGTCC TGAGGGCAAT 
TTTAAAGTTT CCAAACTCCA GGAAGTGGAA GATCTCTTTT TATTGGCAGC CGGAACAGGC 
TTCACACCAA TGGTTACTGT ACTGAATCAC GCTTTAACTC ACATGTCCAG TCTCAGGAAG 
GTGAAGTTAA TGTTCTTCAA TAAAACAGAG GATGACATAA TTTGGAGATG CCAGCTAGAG 
AAATTGGCAT TAAAAGATAA AAGATTTCAT GTTGAATATG TTCTCTCGGC ACCTTCCCCA 
GAATGGAATG GCAAACAGGG ACACGTGTCA CGAGCTCTTC TCTCTGAATT TTTGCAACGA 
AGTTTAGAAA ATTCTAAAGT CTTCCTTTGC ATCTGTGGAC CAACCCCATT TACAGATGAG 
GGGATAAGGT TGCTGCATGA TCTTAACTTT TCCGATGATG AGATTCATGG TTTTACAGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyb5r4 ( XM_006243472.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243534.1 Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31416
Clone ID Related Accession (Same CDS sequence)	XM_006243472.3
Accession Version	XM_006243472.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome b5 reductase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243472.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTTGCCTCC 
CAGGGGCGTA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGATTG 
ACCAAAAGTG GAAAGGACTT CACGGGATTG AAGGGCGGGT TAATTGAAGT AACAGAAGAA 
GAACTTAAGA AGCACAACAA GAAAGACGAC TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AATGTCAGCC CATATATGGA GTATCATCCT GGTGGGGAGG ATGAGCTTAT GAGAGCCGCA 
GGAGCAGATG GAACGGATCT TTTTAATGAG GTGCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAGT GCCTGGTTGG CAGGATGGCT GTGAAGCCCG CTGTTCCGAA AGGCATGCTT 
CCCAAGAGCC AAGTGACAGA CACACTGCCC AGGGAAGGCC CCAGTTCTCC AAGCTATGAT 
TGGTTCCAAA CAGAGTCTTC AGTGACCATT GTTATATACA CCAAGCAGAA GAATATCAAT 
TTAGACTCAG TAATCGTTGA CCTCCAGGAT GACTCCTTGA GAGCAGAAGC AGTCATCAAG 
GATCATTCTT ATCTTATACA TATTGGGTTA AGCCATGAAG TTCAAGAAAA TTTTTCTGTA 
CGGGTTATTG AGAATGTTGG AAAAATAGAG ATTGTCCTAC AAAAGAAAGA AACTGTTTCT 
TGGAAATGTC TTGGTGATCC CCTGGAGAAA CATGATTCTT TTATTCCAAA GAAAGATACA 
GGCTTGTACT ATAGACAGTG CCAGTTAATT TCCAAGGAAG ACGTTACTCA TGACACAAGG 
CTCTTCTGTC TGATGCTGCC TCCCAGTACT CACCTTCAGG TGCCAGTTGG GCAACATGTT 
TACCTCAAGC TCAGTGTCAC AGGTGCAGAA ATAGTAAAGC CATATACACC CGTGTCTGAG 
TCCTTACTCT CAGATTTTAA AGAACCAGTT CTTTCCCCAA ATAAGTACAT CTACTTTTTG 
ATCAAAATCT ATCCTGCTGG ACTCTTCACG CCAGAACTTG ATCGTCTTCA GATTGGAGAT 
TTTGTTTCTG TAAGTGGTCC TGAGGGCAAT TTTAAAGTTT CCAAACTCCA GGAAGTGGAA 
GATCTCTTTT TATTGGCAGC CGGAACAGGC TTCACACCAA TGGTTACTGT ACTGAATCAC 
GCTTTAACTC ACATGTCCAG TCTCAGGAAG GTGAAGTTAA TGTTCTTCAA TAAAACAGAG 
GATGACATAA TTTGGAGATG CCAGCTAGAG AAATTGGCAT TAAAAGATAA AAGATTTCAT 
GTTGAATATG TTCTCTCGGC ACCTTCCCCA GAATGGAATG GCAAACAGGG ACACGTGTCA 
CGAGCTCTTC TCTCTGAATT TTTGCAACGA AGTTTAGAAA ATTCTAAAGT CTTCCTTTGC 
ATCTGTGGAC CAACCCCATT TACAGATGAG GGGATAAGGT TGCTGCATGA TCTTAACTTT 
TCCGATGATG AGATTCATGG TTTTACAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243534.1
CDS	157..1689
Translation	MLNVPSQAFPAPGSQQRVASQGRSKVPLKQGRSLMDWIRLTKSGKDFTGLKGGLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKGMLPKSQVTDTLPREGPSSPSYDWFQTESSVTIVIYTKQKNINLDSVIVDLQDDSLRAEAVIKDHSYLIHIGLSHEVQENFSVRVIENVGKIEIVLQKKETVSWKCLGDPLEKHDSFIPKKDTGLYYRQCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSESLLSDFKEPVLSPNKYIYFLIKIYPAGLFTPELDRLQIGDFVSVSGPEGNFKVSKLQEVEDLFLLAAGTGFTPMVTVLNHALTHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALKDKRFHVEYVLSAPSPEWNGKQGHVSRALLSEFLQRSLENSKVFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	XM_006243472.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243472.3

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTTGCCTCC 
CAGGGGCGTA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGATTG 
ACCAAAAGTG GAAAGGACTT CACGGGATTG AAGGGCGGGT TAATTGAAGT AACAGAAGAA 
GAACTTAAGA AGCACAACAA GAAAGACGAC TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AATGTCAGCC CATATATGGA GTATCATCCT GGTGGGGAGG ATGAGCTTAT GAGAGCCGCA 
GGAGCAGATG GAACGGATCT TTTTAATGAG GTGCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAGT GCCTGGTTGG CAGGATGGCT GTGAAGCCCG CTGTTCCGAA AGGCATGCTT 
CCCAAGAGCC AAGTGACAGA CACACTGCCC AGGGAAGGCC CCAGTTCTCC AAGCTATGAT 
TGGTTCCAAA CAGAGTCTTC AGTGACCATT GTTATATACA CCAAGCAGAA GAATATCAAT 
TTAGACTCAG TAATCGTTGA CCTCCAGGAT GACTCCTTGA GAGCAGAAGC AGTCATCAAG 
GATCATTCTT ATCTTATACA TATTGGGTTA AGCCATGAAG TTCAAGAAAA TTTTTCTGTA 
CGGGTTATTG AGAATGTTGG AAAAATAGAG ATTGTCCTAC AAAAGAAAGA AACTGTTTCT 
TGGAAATGTC TTGGTGATCC CCTGGAGAAA CATGATTCTT TTATTCCAAA GAAAGATACA 
GGCTTGTACT ATAGACAGTG CCAGTTAATT TCCAAGGAAG ACGTTACTCA TGACACAAGG 
CTCTTCTGTC TGATGCTGCC TCCCAGTACT CACCTTCAGG TGCCAGTTGG GCAACATGTT 
TACCTCAAGC TCAGTGTCAC AGGTGCAGAA ATAGTAAAGC CATATACACC CGTGTCTGAG 
TCCTTACTCT CAGATTTTAA AGAACCAGTT CTTTCCCCAA ATAAGTACAT CTACTTTTTG 
ATCAAAATCT ATCCTGCTGG ACTCTTCACG CCAGAACTTG ATCGTCTTCA GATTGGAGAT 
TTTGTTTCTG TAAGTGGTCC TGAGGGCAAT TTTAAAGTTT CCAAACTCCA GGAAGTGGAA 
GATCTCTTTT TATTGGCAGC CGGAACAGGC TTCACACCAA TGGTTACTGT ACTGAATCAC 
GCTTTAACTC ACATGTCCAG TCTCAGGAAG GTGAAGTTAA TGTTCTTCAA TAAAACAGAG 
GATGACATAA TTTGGAGATG CCAGCTAGAG AAATTGGCAT TAAAAGATAA AAGATTTCAT 
GTTGAATATG TTCTCTCGGC ACCTTCCCCA GAATGGAATG GCAAACAGGG ACACGTGTCA 
CGAGCTCTTC TCTCTGAATT TTTGCAACGA AGTTTAGAAA ATTCTAAAGT CTTCCTTTGC 
ATCTGTGGAC CAACCCCATT TACAGATGAG GGGATAAGGT TGCTGCATGA TCTTAACTTT 
TCCGATGATG AGATTCATGG TTTTACAGCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The reductase NCB5OR is responsive to the redox status in beta-cells and is not involved in the ER stress response.
The Biochemical journal404(3)467-76(2007 Jun)
Larade K,Jiang ZG,Dejam A,Zhu H,Bunn HF

Mutagenesis of Glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase.
Biochemistry44(41)13467-76(2005 Oct)
Roma GW,Crowley LJ,Davis CA,Barber MJ

Variation in NCB5OR: studies of relationships to type 2 diabetes, maturity-onset diabetes of the young, and gestational diabetes mellitus.
Diabetes53(11)2992-7(2004 Nov)
Andersen G,Wegner L,Rose CS,Xie J,Zhu H,Larade K,Johansen A,Ek J,Lauenborg J,Drivsholm T,Borch-Johnsen K,Damm P,Hansen T,Bunn HF,Pedersen O

NCB5OR is a novel soluble NAD(P)H reductase localized in the endoplasmic reticulum.
The Journal of biological chemistry279(29)30316-25(2004 Jul)
Zhu H,Larade K,Jackson TA,Xie J,Ladoux A,Acker H,Berchner-Pfannschmidt U,Fandrey J,Cross AR,Lukat-Rodgers GS,Rodgers KR,Bunn HF

Absence of a reductase, NCB5OR, causes insulin-deficient diabetes.
Proceedings of the National Academy of Sciences of the United States of America101(29)10750-5(2004 Jul)
Xie J,Zhu H,Larade K,Ladoux A,Seguritan A,Chu M,Ito S,Bronson RT,Leiter EH,Zhang CY,Rosen ED,Bunn HF

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Cyb5r4 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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