top1l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	top1l
Entrez Gene ID	553496
Full Name	DNA topoisomerase I, like
Synonyms	wu:fc66a02,zgc:136349
General protein information	Preferred Names DNA topoisomerase I, like Names DNA topoisomerase 1 topoisomerase (DNA) I, like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044324.2	NP_001037789.1	DNA topoisomerase 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	TOP1	XP_001088021.1
Bos taurus (cattle)	TOP1	NP_001193416.1
Gallus gallus (chicken)	TOP1	NP_990441.1
Arabidopsis thaliana (thale cress)	TOP1ALPHA	NP_200341.1
Neurospora crassa	NCU09118	XP_958033.2
Canis lupus familiaris (dog)	TOP1	XP_534420.3
Caenorhabditis elegans (roundworm)	top-1	NP_001021578.1
Saccharomyces cerevisiae (baker's yeast)	TOP1	NP_014637.1
Arabidopsis thaliana (thale cress)	TOP1BETA	NP_200342.1
Magnaporthe oryzae (rice blast fungus)	MGG_09917	XP_003710015.1
Kluyveromyces lactis	KLLA0C06116g	XP_452470.1
Eremothecium gossypii	AGOS_ACL008C	NP_983396.2
Xenopus tropicalis (tropical clawed frog)	LOC100496521	XP_002932918.2
Oryza sativa (rice)	Os08g0154600	NP_001061015.1
Mus musculus (house mouse)	Top1	NP_033434.2
Danio rerio (zebrafish)	top1l	NP_001037789.1
Homo sapiens (human)	TOP1	NP_003277.1
Pan troglodytes (chimpanzee)	TOP1	XP_514649.2
Rattus norvegicus (Norway rat)	Top1	NP_072137.1
Drosophila melanogaster (fruit fly)	Top1	NP_001014742.1
Schizosaccharomyces pombe (fission yeast)	top1	NP_596209.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004078	XP_003436707.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.
Leontovich AA, Intine RV, Sarras MP Jr
Journal of diabetes research20162860780(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Environmental acidification triggers oxidative stress and enhances globin expression in zebrafish gills.
Tiedke J, Cubuk C, Burmester T
Biochemical and biophysical research communications441(3)624-9(2013 Nov)

Chronic reduction in cardiac output induces hypoxic signaling in larval zebrafish even at a time when convective oxygen transport is not required.
Kopp R, Schwerte T, Egg M, Sandbichler AM, Egger B, Pelster B
Physiological genomics42A(1)8-23(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following top1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39001	NM_001044324.2 Latest version!	Danio rerio DNA topoisomerase I, like (top1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

top1l ( NM_001044324.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001037789.1 Danio rerio DNA topoisomerase I, like (top1l), mRNA.

Price & Availability↑

CloneID	ODa39001
Clone ID Related Accession (Same CDS sequence)	NM_001044324.2
Accession Version	NM_001044324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC117588.1, CR548630.7 and BC050952.1. On Apr 15, 2009 this sequence version replaced NM_001044324.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01011613.1, GFIL01041679.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGGGG ACCATTCCCA CAACGATTCC CAGATCGACT CGGGATCCAG ACATAATGAT 
TCTCACAAAC ACAAAGATAA ATACAAGGAC AAAGAACACA AACACAAGGA CCACAAGAAA 
GACAAAGAAC GTGAGAAATC AAAGTACAGC AACAGCGAAC ACAGGGACTC CTCGGAAAAA 
AAGCACAGAG ATAAAGATAA AGAGAGGCAG AAGCTCAAAG ATGGATCTTC AGACAAGCAG 
AAGGACAAAC ACAAAGAGAA GAGAAAGGAG GAAAGGTCTT CTGATGACAG GCCTAAAAAA 
GAGAAACAGA ATGGATTCGC AAGCCCTCAC ATTAAATCTG AACCTGACAG CGATGACTTC 
TATCACTCTC CTAAACTAGA GAAGTCTCTG AAGAGAGAGC GGGATGATGA CGATGGTGAT 
TACAAGCCCA AGAAAATTAA AACTGAGGAT GACAGAAGTG AAAAGAAAGC AAAGAAACGG 
AAACAGGAGG AAGAGGACAT AAAGCCCAAA AAGAAAACAA AAGACAAGAA AGGAGAAGTT 
GCCACAGATG GCAAGAAAAA GGCAAAAAAG GAGCCAGAAG AGAAGTGGAA ATGGTGGGAG 
GAAGAAAGGT ACACAGATGG AGTGAAATGG AAGTTTCTGG AGCACAAAGG ACCTGTCTTT 
GCACCACCAT ATGAGCCTTT GCCCAGCAAT GTCAAGTTTT ATTATGACGG GAAGCATATG 
AAACTCAGCC CAAATGCAGA GGAGGTGGCC ACCTTCTTCT CAAAAATGTT GGACCACGAG 
TACACCACTA AAGACATCTT TCGTAAAAAC TTCTTTAAAG ATTGGAGGAA GGAAATGACG 
TCTGAAGAGA AATCCAAAAT CACTGATCTA AATAAGTGTG ACTTCTCTAA TATGAGTGAA 
TATTTCAAAG CTCAATCTGA AGCCAGGAAG GCAATGACTA AAGAAGAAAA ACTGAAAATC 
AAAGAAGAGA ACGAACGCAT TCTGCAGGAG TATGGCTTCT GTATTATGGA CAATCACAAA 
GAGCGCATTG CCAACTTTCG AATTGAGCCT CCAGGCTTGT TCCGTGGAAG AGGTGACCAT 
CCTAAAATGG GCATGCTGAA GCGTCGTATC CGACCCGAAG ACATTATCAT TAACTGCAGC 
AAGGACTCAA AACATCCTAC ACCGCCTCCC GGGACAAAGT GGAAGGAGGT ACGTCATGAC 
AACAAGGTGA CATGGCTGGT GTCCTGGACC GAAAACATCC AAGGCTCCAT CAAATACATC 
ATGCTGAACC CCAGTTCGAG GATCAAGGGA GAGAAGGATT GGCAGAAATA TGAAACAGCC 
CGTCGGTTAA AGAAATGTGT GGATCGGATC CGTTCGCAGT ACCGCGATGA CTGGAAATCA 
AAAGAAATGA AGATCAGACA GCGTGCTGTT GCTCTTTACT TCATTGACAA GCTGGCTTTG 
AGAGCAGGTA ATGAAAAGGA GGAAGGAGAG ACTGCTGACA CAGTGGGCTG CTGCTCACTT 
CGAGTGGAGC ATCTCACTCT GCATCCAGAG ATGGACGGCC AGGAGTTTGT GGTGGAGTTT 
GACTTTCTTG GTAAAGACTC CATTAGATAC TACAACAAGG TCCCTGTAGA GAAGAGGGTT 
TTCAAAAATC TCCAGCTTTT CATGGAGGAT AAGGAGCCAG AGGATGACCT CTTTGACCGT 
CTCAATACCT CCATTTTAAA CAAGCATCTT CAGGAGTTGA TGGACGGCCT TACGGCTAAA 
GTGTTCCGTA CTTACAACGC CTCCATCACA CTACAGCAGC AGCTGAACGA ACTCACTAAC 
TCTGAAGATA ATGTTCCTGC GAAGATCCTC TCCTACAATC GGGCCAACCG AGCGGTAGCC 
ATCCTGTGTA ACCACCAGAG GGCACCACCA AAGACCTTTG AAAAGTCCAT GCAGAATCTT 
CAGACCAAGA TTGACGCAAA GAAGGAGCAG CTTGTTGAAG CTAAAAGAGA GCTGAAAGGT 
GCCAAGGCTG ACGCCAAAGT ACGCAAAGAT GAGAAATTCA AGAAAGCTGT GGAGGCCAAG 
AAGAAAGCGC TTCAGAGGGT GGAGGAGCAG CTGATGAAGC TGGAGGTTCA GGCCACCGAC 
CGCGAGGAGA ACAAACAGAT CGCTCTGGGG ACCTCCAAAC TCAACTACCT GGATCCACGC 
ATCTCTGTAG CCTGGTGTAA GAAGTTCGGC ATCCCGATAG AGAAGATCTA CAACAAAACT 
CAGCGTGAGA AATTTGCCTG GGCGATTGAC ATGGCTGAAA AAGACTATGA ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037789.1
CDS	121..2397
Translation	MSGDHSHNDSQIDSGSRHNDSHKHKDKYKDKEHKHKDHKKDKEREKSKYSNSEHRDSSEKKHRDKDKERQKLKDGSSDKQKDKHKEKRKEERSSDDRPKKEKQNGFASPHIKSEPDSDDFYHSPKLEKSLKRERDDDDGDYKPKKIKTEDDRSEKKAKKRKQEEEDIKPKKKTKDKKGEVATDGKKKAKKEPEEKWKWWEEERYTDGVKWKFLEHKGPVFAPPYEPLPSNVKFYYDGKHMKLSPNAEEVATFFSKMLDHEYTTKDIFRKNFFKDWRKEMTSEEKSKITDLNKCDFSNMSEYFKAQSEARKAMTKEEKLKIKEENERILQEYGFCIMDNHKERIANFRIEPPGLFRGRGDHPKMGMLKRRIRPEDIIINCSKDSKHPTPPPGTKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDRIRSQYRDDWKSKEMKIRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHLTLHPEMDGQEFVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMEDKEPEDDLFDRLNTSILNKHLQELMDGLTAKVFRTYNASITLQQQLNELTNSEDNVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMQNLQTKIDAKKEQLVEAKRELKGAKADAKVRKDEKFKKAVEAKKKALQRVEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKFGIPIEKIYNKTQREKFAWAIDMAEKDYEF

Target ORF information:

RefSeq Version	NM_001044324.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DNA topoisomerase I, like (top1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044324.2

ORF Insert Sequence:

ATGAGTGGGG ACCATTCCCA CAACGATTCC CAGATCGACT CGGGATCCAG ACATAATGAT 
TCTCACAAAC ACAAAGATAA ATACAAGGAC AAAGAACACA AACACAAGGA CCACAAGAAA 
GACAAAGAAC GTGAGAAATC AAAGTACAGC AACAGCGAAC ACAGGGACTC CTCGGAAAAA 
AAGCACAGAG ATAAAGATAA AGAGAGGCAG AAGCTCAAAG ATGGATCTTC AGACAAGCAG 
AAGGACAAAC ACAAAGAGAA GAGAAAGGAG GAAAGGTCTT CTGATGACAG GCCTAAAAAA 
GAGAAACAGA ATGGATTCGC AAGCCCTCAC ATTAAATCTG AACCTGACAG CGATGACTTC 
TATCACTCTC CTAAACTAGA GAAGTCTCTG AAGAGAGAGC GGGATGATGA CGATGGTGAT 
TACAAGCCCA AGAAAATTAA AACTGAGGAT GACAGAAGTG AAAAGAAAGC AAAGAAACGG 
AAACAGGAGG AAGAGGACAT AAAGCCCAAA AAGAAAACAA AAGACAAGAA AGGAGAAGTT 
GCCACAGATG GCAAGAAAAA GGCAAAAAAG GAGCCAGAAG AGAAGTGGAA ATGGTGGGAG 
GAAGAAAGGT ACACAGATGG AGTGAAATGG AAGTTTCTGG AGCACAAAGG ACCTGTCTTT 
GCACCACCAT ATGAGCCTTT GCCCAGCAAT GTCAAGTTTT ATTATGACGG GAAGCATATG 
AAACTCAGCC CAAATGCAGA GGAGGTGGCC ACCTTCTTCT CAAAAATGTT GGACCACGAG 
TACACCACTA AAGACATCTT TCGTAAAAAC TTCTTTAAAG ATTGGAGGAA GGAAATGACG 
TCTGAAGAGA AATCCAAAAT CACTGATCTA AATAAGTGTG ACTTCTCTAA TATGAGTGAA 
TATTTCAAAG CTCAATCTGA AGCCAGGAAG GCAATGACTA AAGAAGAAAA ACTGAAAATC 
AAAGAAGAGA ACGAACGCAT TCTGCAGGAG TATGGCTTCT GTATTATGGA CAATCACAAA 
GAGCGCATTG CCAACTTTCG AATTGAGCCT CCAGGCTTGT TCCGTGGAAG AGGTGACCAT 
CCTAAAATGG GCATGCTGAA GCGTCGTATC CGACCCGAAG ACATTATCAT TAACTGCAGC 
AAGGACTCAA AACATCCTAC ACCGCCTCCC GGGACAAAGT GGAAGGAGGT ACGTCATGAC 
AACAAGGTGA CATGGCTGGT GTCCTGGACC GAAAACATCC AAGGCTCCAT CAAATACATC 
ATGCTGAACC CCAGTTCGAG GATCAAGGGA GAGAAGGATT GGCAGAAATA TGAAACAGCC 
CGTCGGTTAA AGAAATGTGT GGATCGGATC CGTTCGCAGT ACCGCGATGA CTGGAAATCA 
AAAGAAATGA AGATCAGACA GCGTGCTGTT GCTCTTTACT TCATTGACAA GCTGGCTTTG 
AGAGCAGGTA ATGAAAAGGA GGAAGGAGAG ACTGCTGACA CAGTGGGCTG CTGCTCACTT 
CGAGTGGAGC ATCTCACTCT GCATCCAGAG ATGGACGGCC AGGAGTTTGT GGTGGAGTTT 
GACTTTCTTG GTAAAGACTC CATTAGATAC TACAACAAGG TCCCTGTAGA GAAGAGGGTT 
TTCAAAAATC TCCAGCTTTT CATGGAGGAT AAGGAGCCAG AGGATGACCT CTTTGACCGT 
CTCAATACCT CCATTTTAAA CAAGCATCTT CAGGAGTTGA TGGACGGCCT TACGGCTAAA 
GTGTTCCGTA CTTACAACGC CTCCATCACA CTACAGCAGC AGCTGAACGA ACTCACTAAC 
TCTGAAGATA ATGTTCCTGC GAAGATCCTC TCCTACAATC GGGCCAACCG AGCGGTAGCC 
ATCCTGTGTA ACCACCAGAG GGCACCACCA AAGACCTTTG AAAAGTCCAT GCAGAATCTT 
CAGACCAAGA TTGACGCAAA GAAGGAGCAG CTTGTTGAAG CTAAAAGAGA GCTGAAAGGT 
GCCAAGGCTG ACGCCAAAGT ACGCAAAGAT GAGAAATTCA AGAAAGCTGT GGAGGCCAAG 
AAGAAAGCGC TTCAGAGGGT GGAGGAGCAG CTGATGAAGC TGGAGGTTCA GGCCACCGAC 
CGCGAGGAGA ACAAACAGAT CGCTCTGGGG ACCTCCAAAC TCAACTACCT GGATCCACGC 
ATCTCTGTAG CCTGGTGTAA GAAGTTCGGC ATCCCGATAG AGAAGATCTA CAACAAAACT 
CAGCGTGAGA AATTTGCCTG GGCGATTGAC ATGGCTGAAA AAGACTATGA ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.
Journal of diabetes research20162860780(2016)
Leontovich AA,Intine RV,Sarras MP Jr

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Environmental acidification triggers oxidative stress and enhances globin expression in zebrafish gills.
Biochemical and biophysical research communications441(3)624-9(2013 Nov)
Tiedke J,Cubuk C,Burmester T

Chronic reduction in cardiac output induces hypoxic signaling in larval zebrafish even at a time when convective oxygen transport is not required.
Physiological genomics42A(1)8-23(2010 Sep)
Kopp R,Schwerte T,Egg M,Sandbichler AM,Egger B,Pelster B

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top1l cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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