WAPLA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	WAPLA
Entrez Gene ID	553345
Full Name	WAPL cohesin release factor a
Synonyms	im:7143545,kiaa0261,wapala
General protein information	Preferred Names WAPL cohesin release factor a Names wings apart-like protein homolog wings apart-like homolog a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_683513.7	XP_688605.5	wings apart-like protein homolog
XM_021473179.1	XP_021328854.1	wings apart-like protein homolog
XM_683513.6	XP_688605.5	wings apart-like protein homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	WAPAL	NP_055860.1
Pan troglodytes (chimpanzee)	WAPAL	XP_003312721.1
Macaca mulatta (Rhesus monkey)	WAPAL	XP_002805691.1
Canis lupus familiaris (dog)	WAPAL	XP_864834.3
Mus musculus (house mouse)	Wapal	NP_001004436.2
Gallus gallus (chicken)	WAPAL	NP_001186440.1
Danio rerio (zebrafish)	wapala	XP_688605.5
Xenopus tropicalis (tropical clawed frog)	wapal	XP_002937603.2
Bos taurus (cattle)	WAPAL	XP_005226580.1
Caenorhabditis elegans (roundworm)	wapl-1	NP_500567.1
Rattus norvegicus (Norway rat)	Wapal	XP_006252785.1

Gene Ontology

The following WAPLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WAPLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22552	XM_021473179.1 Latest version!	Danio rerio WAPL cohesin release factor a (wapla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22552	XM_683513.7 Latest version!	Danio rerio WAPL cohesin release factor a (wapla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa22552	XM_683513.6	Danio rerio WAPL cohesin release factor a (wapla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WAPLA ( XM_683513.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_688605.5 Danio rerio WAPL cohesin release factor a (wapla), mRNA.

Price & Availability↑

CloneID	ODa22552
Clone ID Related Accession (Same CDS sequence)	XM_683513.7 , XM_021473179.1 , XM_683513.6
Accession Version	XM_683513.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	wings apart-like protein homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_683513.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCAA GATTTAGTAA AACGTACACG CGGAAGGGAG GAGAAGCCAG CTCAAAGTTT 
GACGAAGTGT TCTCTAACAA GAAAGCTACA CTCACCACCA AATGGGGAGA GTCCACCTAC 
AAGGCGCATT TGGGCATCAA ACGACCCTCA TTCAAGCCAG ATGTTTCAGA GCTGACCAAA 
CGACCCCGCT TTGATGATGA CGACAGCTCA GAGGATCCGT TTGGCTTTGA CAGCGATGAT 
GAGTCCAAAA CAGTCACATC CAGAGATACT GCTCAATCTG AGATGGACTC GGATACTGCA 
GGACCCAAGA ATGAGCAGTT AAAGACCCGG ATGGAAAAGC CCAGCGGGGG GACACTGGTG 
ACCAGTACTT CTTCCTTGGG GAGTAGTACA AACATCAGCA GAGGGGCGGT TGGCGTGAAT 
AGCCCAGCTA AAACTTCTCC AGCCTCTTCT TATGTCACTG GTACTGTAGG AACCTGGAGC 
AAATCCAATG TTACAGTAAG TAAACAGCTC TCCCAGGTCT CCAGCTTGAA GATCCCAAGT 
AGAGGCATCT CCAATGACTG GGACAGTTTT GGGGAGGACT CTCCTCCTGG AGCCGGTGAG 
GGCAGTTCTA CTCAGACTAG TAACTCTTCT GATGAGCTCA AGAGTGCAGA GCAGTGCCCT 
GCTGAACCTG AACCGCCAGT GGAGCAACCT GTTGACTTTG AGCAGCTTCC GCTTCTTAAA 
CCATCTAGCG ACAGAGTGTA CAGACGACCA CGGGGTAAAG AGACCGAAAG TTCAGCTGCT 
GGAGATACAA TTCCTGCTGT GAGTTCGGTG TCAGCTCCTG CACCAGTGAT GAGCAGCAAT 
GCAGGGGTTG CTGGGAGTTC TGGGGTGAAG CCTCCTGCAG GTCGGGGTAG AGTGAGGGAT 
TACACCGTTC TTCACCCGTC TTGTGTGTCC GTGTGTAATG TGACCATCCA GGACTCCATT 
GAAAGGAGCG TGGATGATTT TGTTATGCCA GTGCAAGCTG ACATTGGAGA AGCAGGAACC 
TTCAGGAGGA AAGCAGACAC ACAGGCTCCT AAACCTACCA GGTTTAGACC CACTCAGAGC 
AAAAGTAAAA AGGAGACAAA ACTGGAGTTT TTTGGCTTTG AAGAGAACGA GGCTCAGGAC 
GCAGATGGAG ACGCCACTGG CTCTGGCAGC ACCAGCTACA AGATCAAATA CTTTGGTTTT 
GATGATCTGA GTGAGAGTGA CAGCGAGGAT GATGATGATT CAGCAGCTAG AGAGAGGAAA 
AAGAATAAGA AAGCAGAGGC AGAGGCTTTG TCAACCGGCA CAGGAAGTCC ACAGTTCAGC 
GATTCGCAGG AAAGTCAACA GAGTCAAAGC AGCCTAAGTA CAGATTCTCA GGAGTCACTG 
GATGAAACGG GAGCTACGGG TTCACAGAAA GAACGACACA GAAAGCAAAG CAACAAGTCA 
AAGGAGATCG GCCGCAAGAT CTTCAGTGGA CCAAAAAAAT CTCCTGCTAA AGCTGTATAC 
AACGCTCGTC ACTGGAATCA GCCTGAGTCG GAGGAAGTTC CATCTCCTGC TTCTCATACT 
GTGGCTGTAC CGAAGCCAAG TGCATCCAGC AGCAGCACAA GCAGCAGCAA AGACACAAAT 
TCCCATAAAG ATGATGGAGT GTTTAAAGCT CCACCGCCTC CACCTAAAGT CATCAAGTCT 
GTCACCATCC CCACTGAGCT GAACCAGGAC ATCGTCACTG CACTGAAATG CAGGAAGGAG 
CACAAGGAGT TGTATACAGT GGTCCAACAT GTGAAGCACT TTAATGATGT GGTGGAGTTT 
GGGGAGAATC AGGAGTTCAC AGATGACTTT GAGTATCTAG AGACGGGCTT GAAGAGCACA 
CAACCTCTCA ACACACGCTG CCTTAGTATA ATCAGTCTGG CCACACGCTG TGCCATGCCA 
AGCTTCCGAA TGCATCTGAG AGCCAGAGGG AAGGTGGCGC AGGTCTTTAA GATGCTCAGT 
GATGCTCAAC AGCACCCGAA TATGGCTCTC TGCACGGCGT CTCTGATGTA TATATTGAGT 
CGAGACCGGC TGAATATGGA TCTGGATCGG TCTTGTCTGG AGCTGATGAT CCGGCTGCTG 
GAAATGGAGC AGGACGACTC TGTAGCGGAC CAGTTAACAG CTAAAGAGAT GAGCAAAGTA 
AAAGAGAAAA TCAGGAAACT CTGTGAAACA GTCCACAACA AGCATCTGGA TCTGGAGAAC 
ATCACAACTG GTCATTTAGC AATGGAAACG CTGCTGTCTC TGACGTCGAA GCGAGCTGGA 
GACTGGTTTA AGGAGGAGCT GCGTTTACTT GGGGGACTGG ACCACATCGT AGATAAAGTA 
AAAGAGTGTG TGGAGAACTT GAATAAAGAA GAAGATAAAG AGAGTCTAGT GGCGTCACTC 
TGGGGAGCAG AACGCTGCTT GCGAGTTCTG GAGAGCGTTA CAGTTCATAA CCCTGAGAAC 
CAGGCTTATC TGATTGCCTA CAAAGACTCC CAGCTCATAA TGTCCTCAGC AAGGGCCCTG 
CAGCACTGTG AGCAGATGAT CCAGCGTTAC AGCCGAGAGG TCTCTTCATC ATCGAGCTCT 
ACACTCCCGC AGAGCAGTCA CAGTAATGTG GGCAAAGCCG TGGAGGATTG CATGAGAGCC 
GTGATCGGAG TTCTGCTCAA CCTCACACAT GATAACGAGT GGGGAAGCAC TAAAACAGGA 
GAGCAGGACG GCCTGATACT GACCGCTCTG GACTGTGTTC TGAAGGTCCC GCGCTATTTG 
CCTCAGGAAC AGAGATTTGA TATCCGTGTA CTGGGTCTGG GTCTACTGAT TAATCTGGTG 
GAGTACAGTG CCAGAAACAG AGAGTGTCTG GTGGAGATGG AGGTGGACAT GAGCTACCTA 
ATGGAGGCTG AGGCTGTCGT TAAGATAGAG AACGATGTGA AGACAGAGGA ACAAAAACGC 
GATCAAATGG AAGAAGAAGA AAAGCGAAAA GCGACGCTAA AATCATTAAA TGCCTTGGCT 
GCTCTGGTTC AGCTCTTCCT CGAACGGGAG CGAGCAGCTG TGCTTGCTGA GGCACAGACT 
GATGACCTCA TAAGTGAGGC ACCCAAATCT CAGGCAGACC AGAGCGGAGA GTGGCAAGAA 
ACATCTGGAG AGATCCAGTG GGTTGCCTCT GAAACCAATG ACACAAATGA CAAGAAAGAG 
GAGGAAGAAG AAGAGCTCGA CCTCAACAAA GCCTTGCAGC ATGCTGGGAA ACACATGGAG 
GACAGCATTG TGGCGTCTTA CTCAGCTCTT CTGCTTGGGT GTCTCTGTCA GGGGAGTCCG 
ATGAATGTGA AAACCGTACG GGAAAATCTT CCTAAAGGAG ATTTCTCCAT CATGACTGAA 
ATGCTGAAGA AATTCCTTAA TTTTATGAAC CTGACGTGTT CTGTTGGAAC CACAGGACAG 
AAATCTATCT CACGGGTGAT TGATTATTTG GAGCACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688605.5
CDS	268..3729
Translation	MTSRFSKTYTRKGGEASSKFDEVFSNKKATLTTKWGESTYKAHLGIKRPSFKPDVSELTKRPRFDDDDSSEDPFGFDSDDESKTVTSRDTAQSEMDSDTAGPKNEQLKTRMEKPSGGTLVTSTSSLGSSTNISRGAVGVNSPAKTSPASSYVTGTVGTWSKSNVTVSKQLSQVSSLKIPSRGISNDWDSFGEDSPPGAGEGSSTQTSNSSDELKSAEQCPAEPEPPVEQPVDFEQLPLLKPSSDRVYRRPRGKETESSAAGDTIPAVSSVSAPAPVMSSNAGVAGSSGVKPPAGRGRVRDYTVLHPSCVSVCNVTIQDSIERSVDDFVMPVQADIGEAGTFRRKADTQAPKPTRFRPTQSKSKKETKLEFFGFEENEAQDADGDATGSGSTSYKIKYFGFDDLSESDSEDDDDSAARERKKNKKAEAEALSTGTGSPQFSDSQESQQSQSSLSTDSQESLDETGATGSQKERHRKQSNKSKEIGRKIFSGPKKSPAKAVYNARHWNQPESEEVPSPASHTVAVPKPSASSSSTSSSKDTNSHKDDGVFKAPPPPPKVIKSVTIPTELNQDIVTALKCRKEHKELYTVVQHVKHFNDVVEFGENQEFTDDFEYLETGLKSTQPLNTRCLSIISLATRCAMPSFRMHLRARGKVAQVFKMLSDAQQHPNMALCTASLMYILSRDRLNMDLDRSCLELMIRLLEMEQDDSVADQLTAKEMSKVKEKIRKLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVENLNKEEDKESLVASLWGAERCLRVLESVTVHNPENQAYLIAYKDSQLIMSSARALQHCEQMIQRYSREVSSSSSSTLPQSSHSNVGKAVEDCMRAVIGVLLNLTHDNEWGSTKTGEQDGLILTALDCVLKVPRYLPQEQRFDIRVLGLGLLINLVEYSARNRECLVEMEVDMSYLMEAEAVVKIENDVKTEEQKRDQMEEEEKRKATLKSLNALAALVQLFLERERAAVLAEAQTDDLISEAPKSQADQSGEWQETSGEIQWVASETNDTNDKKEEEEEELDLNKALQHAGKHMEDSIVASYSALLLGCLCQGSPMNVKTVRENLPKGDFSIMTEMLKKFLNFMNLTCSVGTTGQKSISRVIDYLEHC

Target ORF information:

RefSeq Version	XM_683513.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WAPL cohesin release factor a (wapla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683513.7

ORF Insert Sequence:

ATGACATCAA GATTTAGTAA AACGTACACG CGGAAGGGAG GAGAAGCCAG CTCAAAGTTT 
GACGAAGTGT TCTCTAACAA GAAAGCTACA CTCACCACCA AATGGGGAGA GTCCACCTAC 
AAGGCGCATT TGGGCATCAA ACGACCCTCA TTCAAGCCAG ATGTTTCAGA GCTGACCAAA 
CGACCCCGCT TTGATGATGA CGACAGCTCA GAGGATCCGT TTGGCTTTGA CAGCGATGAT 
GAGTCCAAAA CAGTCACATC CAGAGATACT GCTCAATCTG AGATGGACTC GGATACTGCA 
GGACCCAAGA ATGAGCAGTT AAAGACCCGG ATGGAAAAGC CCAGCGGGGG GACACTGGTG 
ACCAGTACTT CTTCCTTGGG GAGTAGTACA AACATCAGCA GAGGGGCGGT TGGCGTGAAT 
AGCCCAGCTA AAACTTCTCC AGCCTCTTCT TATGTCACTG GTACTGTAGG AACCTGGAGC 
AAATCCAATG TTACAGTAAG TAAACAGCTC TCCCAGGTCT CCAGCTTGAA GATCCCAAGT 
AGAGGCATCT CCAATGACTG GGACAGTTTT GGGGAGGACT CTCCTCCTGG AGCCGGTGAG 
GGCAGTTCTA CTCAGACTAG TAACTCTTCT GATGAGCTCA AGAGTGCAGA GCAGTGCCCT 
GCTGAACCTG AACCGCCAGT GGAGCAACCT GTTGACTTTG AGCAGCTTCC GCTTCTTAAA 
CCATCTAGCG ACAGAGTGTA CAGACGACCA CGGGGTAAAG AGACCGAAAG TTCAGCTGCT 
GGAGATACAA TTCCTGCTGT GAGTTCGGTG TCAGCTCCTG CACCAGTGAT GAGCAGCAAT 
GCAGGGGTTG CTGGGAGTTC TGGGGTGAAG CCTCCTGCAG GTCGGGGTAG AGTGAGGGAT 
TACACCGTTC TTCACCCGTC TTGTGTGTCC GTGTGTAATG TGACCATCCA GGACTCCATT 
GAAAGGAGCG TGGATGATTT TGTTATGCCA GTGCAAGCTG ACATTGGAGA AGCAGGAACC 
TTCAGGAGGA AAGCAGACAC ACAGGCTCCT AAACCTACCA GGTTTAGACC CACTCAGAGC 
AAAAGTAAAA AGGAGACAAA ACTGGAGTTT TTTGGCTTTG AAGAGAACGA GGCTCAGGAC 
GCAGATGGAG ACGCCACTGG CTCTGGCAGC ACCAGCTACA AGATCAAATA CTTTGGTTTT 
GATGATCTGA GTGAGAGTGA CAGCGAGGAT GATGATGATT CAGCAGCTAG AGAGAGGAAA 
AAGAATAAGA AAGCAGAGGC AGAGGCTTTG TCAACCGGCA CAGGAAGTCC ACAGTTCAGC 
GATTCGCAGG AAAGTCAACA GAGTCAAAGC AGCCTAAGTA CAGATTCTCA GGAGTCACTG 
GATGAAACGG GAGCTACGGG TTCACAGAAA GAACGACACA GAAAGCAAAG CAACAAGTCA 
AAGGAGATCG GCCGCAAGAT CTTCAGTGGA CCAAAAAAAT CTCCTGCTAA AGCTGTATAC 
AACGCTCGTC ACTGGAATCA GCCTGAGTCG GAGGAAGTTC CATCTCCTGC TTCTCATACT 
GTGGCTGTAC CGAAGCCAAG TGCATCCAGC AGCAGCACAA GCAGCAGCAA AGACACAAAT 
TCCCATAAAG ATGATGGAGT GTTTAAAGCT CCACCGCCTC CACCTAAAGT CATCAAGTCT 
GTCACCATCC CCACTGAGCT GAACCAGGAC ATCGTCACTG CACTGAAATG CAGGAAGGAG 
CACAAGGAGT TGTATACAGT GGTCCAACAT GTGAAGCACT TTAATGATGT GGTGGAGTTT 
GGGGAGAATC AGGAGTTCAC AGATGACTTT GAGTATCTAG AGACGGGCTT GAAGAGCACA 
CAACCTCTCA ACACACGCTG CCTTAGTATA ATCAGTCTGG CCACACGCTG TGCCATGCCA 
AGCTTCCGAA TGCATCTGAG AGCCAGAGGG AAGGTGGCGC AGGTCTTTAA GATGCTCAGT 
GATGCTCAAC AGCACCCGAA TATGGCTCTC TGCACGGCGT CTCTGATGTA TATATTGAGT 
CGAGACCGGC TGAATATGGA TCTGGATCGG TCTTGTCTGG AGCTGATGAT CCGGCTGCTG 
GAAATGGAGC AGGACGACTC TGTAGCGGAC CAGTTAACAG CTAAAGAGAT GAGCAAAGTA 
AAAGAGAAAA TCAGGAAACT CTGTGAAACA GTCCACAACA AGCATCTGGA TCTGGAGAAC 
ATCACAACTG GTCATTTAGC AATGGAAACG CTGCTGTCTC TGACGTCGAA GCGAGCTGGA 
GACTGGTTTA AGGAGGAGCT GCGTTTACTT GGGGGACTGG ACCACATCGT AGATAAAGTA 
AAAGAGTGTG TGGAGAACTT GAATAAAGAA GAAGATAAAG AGAGTCTAGT GGCGTCACTC 
TGGGGAGCAG AACGCTGCTT GCGAGTTCTG GAGAGCGTTA CAGTTCATAA CCCTGAGAAC 
CAGGCTTATC TGATTGCCTA CAAAGACTCC CAGCTCATAA TGTCCTCAGC AAGGGCCCTG 
CAGCACTGTG AGCAGATGAT CCAGCGTTAC AGCCGAGAGG TCTCTTCATC ATCGAGCTCT 
ACACTCCCGC AGAGCAGTCA CAGTAATGTG GGCAAAGCCG TGGAGGATTG CATGAGAGCC 
GTGATCGGAG TTCTGCTCAA CCTCACACAT GATAACGAGT GGGGAAGCAC TAAAACAGGA 
GAGCAGGACG GCCTGATACT GACCGCTCTG GACTGTGTTC TGAAGGTCCC GCGCTATTTG 
CCTCAGGAAC AGAGATTTGA TATCCGTGTA CTGGGTCTGG GTCTACTGAT TAATCTGGTG 
GAGTACAGTG CCAGAAACAG AGAGTGTCTG GTGGAGATGG AGGTGGACAT GAGCTACCTA 
ATGGAGGCTG AGGCTGTCGT TAAGATAGAG AACGATGTGA AGACAGAGGA ACAAAAACGC 
GATCAAATGG AAGAAGAAGA AAAGCGAAAA GCGACGCTAA AATCATTAAA TGCCTTGGCT 
GCTCTGGTTC AGCTCTTCCT CGAACGGGAG CGAGCAGCTG TGCTTGCTGA GGCACAGACT 
GATGACCTCA TAAGTGAGGC ACCCAAATCT CAGGCAGACC AGAGCGGAGA GTGGCAAGAA 
ACATCTGGAG AGATCCAGTG GGTTGCCTCT GAAACCAATG ACACAAATGA CAAGAAAGAG 
GAGGAAGAAG AAGAGCTCGA CCTCAACAAA GCCTTGCAGC ATGCTGGGAA ACACATGGAG 
GACAGCATTG TGGCGTCTTA CTCAGCTCTT CTGCTTGGGT GTCTCTGTCA GGGGAGTCCG 
ATGAATGTGA AAACCGTACG GGAAAATCTT CCTAAAGGAG ATTTCTCCAT CATGACTGAA 
ATGCTGAAGA AATTCCTTAA TTTTATGAAC CTGACGTGTT CTGTTGGAAC CACAGGACAG 
AAATCTATCT CACGGGTGAT TGATTATTTG GAGCACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPLA ( XM_021473179.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328854.1 Danio rerio WAPL cohesin release factor a (wapla), mRNA.

Price & Availability↑

CloneID	ODa22552
Clone ID Related Accession (Same CDS sequence)	XM_683513.7 , XM_021473179.1 , XM_683513.6
Accession Version	XM_021473179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	wings apart-like protein homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394992.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCAA GATTTAGTAA AACGTACACG CGGAAGGGAG GAGAAGCCAG CTCAAAGTTT 
GACGAAGTGT TCTCTAACAA GAAAGCTACA CTCACCACCA AATGGGGAGA GTCCACCTAC 
AAGGCGCATT TGGGCATCAA ACGACCCTCA TTCAAGCCAG ATGTTTCAGA GCTGACCAAA 
CGACCCCGCT TTGATGATGA CGACAGCTCA GAGGATCCGT TTGGCTTTGA CAGCGATGAT 
GAGTCCAAAA CAGTCACATC CAGAGATACT GCTCAATCTG AGATGGACTC GGATACTGCA 
GGACCCAAGA ATGAGCAGTT AAAGACCCGG ATGGAAAAGC CCAGCGGGGG GACACTGGTG 
ACCAGTACTT CTTCCTTGGG GAGTAGTACA AACATCAGCA GAGGGGCGGT TGGCGTGAAT 
AGCCCAGCTA AAACTTCTCC AGCCTCTTCT TATGTCACTG GTACTGTAGG AACCTGGAGC 
AAATCCAATG TTACAGTAAG TAAACAGCTC TCCCAGGTCT CCAGCTTGAA GATCCCAAGT 
AGAGGCATCT CCAATGACTG GGACAGTTTT GGGGAGGACT CTCCTCCTGG AGCCGGTGAG 
GGCAGTTCTA CTCAGACTAG TAACTCTTCT GATGAGCTCA AGAGTGCAGA GCAGTGCCCT 
GCTGAACCTG AACCGCCAGT GGAGCAACCT GTTGACTTTG AGCAGCTTCC GCTTCTTAAA 
CCATCTAGCG ACAGAGTGTA CAGACGACCA CGGGGTAAAG AGACCGAAAG TTCAGCTGCT 
GGAGATACAA TTCCTGCTGT GAGTTCGGTG TCAGCTCCTG CACCAGTGAT GAGCAGCAAT 
GCAGGGGTTG CTGGGAGTTC TGGGGTGAAG CCTCCTGCAG GTCGGGGTAG AGTGAGGGAT 
TACACCGTTC TTCACCCGTC TTGTGTGTCC GTGTGTAATG TGACCATCCA GGACTCCATT 
GAAAGGAGCG TGGATGATTT TGTTATGCCA GTGCAAGCTG ACATTGGAGA AGCAGGAACC 
TTCAGGAGGA AAGCAGACAC ACAGGCTCCT AAACCTACCA GGTTTAGACC CACTCAGAGC 
AAAAGTAAAA AGGAGACAAA ACTGGAGTTT TTTGGCTTTG AAGAGAACGA GGCTCAGGAC 
GCAGATGGAG ACGCCACTGG CTCTGGCAGC ACCAGCTACA AGATCAAATA CTTTGGTTTT 
GATGATCTGA GTGAGAGTGA CAGCGAGGAT GATGATGATT CAGCAGCTAG AGAGAGGAAA 
AAGAATAAGA AAGCAGAGGC AGAGGCTTTG TCAACCGGCA CAGGAAGTCC ACAGTTCAGC 
GATTCGCAGG AAAGTCAACA GAGTCAAAGC AGCCTAAGTA CAGATTCTCA GGAGTCACTG 
GATGAAACGG GAGCTACGGG TTCACAGAAA GAACGACACA GAAAGCAAAG CAACAAGTCA 
AAGGAGATCG GCCGCAAGAT CTTCAGTGGA CCAAAAAAAT CTCCTGCTAA AGCTGTATAC 
AACGCTCGTC ACTGGAATCA GCCTGAGTCG GAGGAAGTTC CATCTCCTGC TTCTCATACT 
GTGGCTGTAC CGAAGCCAAG TGCATCCAGC AGCAGCACAA GCAGCAGCAA AGACACAAAT 
TCCCATAAAG ATGATGGAGT GTTTAAAGCT CCACCGCCTC CACCTAAAGT CATCAAGTCT 
GTCACCATCC CCACTGAGCT GAACCAGGAC ATCGTCACTG CACTGAAATG CAGGAAGGAG 
CACAAGGAGT TGTATACAGT GGTCCAACAT GTGAAGCACT TTAATGATGT GGTGGAGTTT 
GGGGAGAATC AGGAGTTCAC AGATGACTTT GAGTATCTAG AGACGGGCTT GAAGAGCACA 
CAACCTCTCA ACACACGCTG CCTTAGTATA ATCAGTCTGG CCACACGCTG TGCCATGCCA 
AGCTTCCGAA TGCATCTGAG AGCCAGAGGG AAGGTGGCGC AGGTCTTTAA GATGCTCAGT 
GATGCTCAAC AGCACCCGAA TATGGCTCTC TGCACGGCGT CTCTGATGTA TATATTGAGT 
CGAGACCGGC TGAATATGGA TCTGGATCGG TCTTGTCTGG AGCTGATGAT CCGGCTGCTG 
GAAATGGAGC AGGACGACTC TGTAGCGGAC CAGTTAACAG CTAAAGAGAT GAGCAAAGTA 
AAAGAGAAAA TCAGGAAACT CTGTGAAACA GTCCACAACA AGCATCTGGA TCTGGAGAAC 
ATCACAACTG GTCATTTAGC AATGGAAACG CTGCTGTCTC TGACGTCGAA GCGAGCTGGA 
GACTGGTTTA AGGAGGAGCT GCGTTTACTT GGGGGACTGG ACCACATCGT AGATAAAGTA 
AAAGAGTGTG TGGAGAACTT GAATAAAGAA GAAGATAAAG AGAGTCTAGT GGCGTCACTC 
TGGGGAGCAG AACGCTGCTT GCGAGTTCTG GAGAGCGTTA CAGTTCATAA CCCTGAGAAC 
CAGGCTTATC TGATTGCCTA CAAAGACTCC CAGCTCATAA TGTCCTCAGC AAGGGCCCTG 
CAGCACTGTG AGCAGATGAT CCAGCGTTAC AGCCGAGAGG TCTCTTCATC ATCGAGCTCT 
ACACTCCCGC AGAGCAGTCA CAGTAATGTG GGCAAAGCCG TGGAGGATTG CATGAGAGCC 
GTGATCGGAG TTCTGCTCAA CCTCACACAT GATAACGAGT GGGGAAGCAC TAAAACAGGA 
GAGCAGGACG GCCTGATACT GACCGCTCTG GACTGTGTTC TGAAGGTCCC GCGCTATTTG 
CCTCAGGAAC AGAGATTTGA TATCCGTGTA CTGGGTCTGG GTCTACTGAT TAATCTGGTG 
GAGTACAGTG CCAGAAACAG AGAGTGTCTG GTGGAGATGG AGGTGGACAT GAGCTACCTA 
ATGGAGGCTG AGGCTGTCGT TAAGATAGAG AACGATGTGA AGACAGAGGA ACAAAAACGC 
GATCAAATGG AAGAAGAAGA AAAGCGAAAA GCGACGCTAA AATCATTAAA TGCCTTGGCT 
GCTCTGGTTC AGCTCTTCCT CGAACGGGAG CGAGCAGCTG TGCTTGCTGA GGCACAGACT 
GATGACCTCA TAAGTGAGGC ACCCAAATCT CAGGCAGACC AGAGCGGAGA GTGGCAAGAA 
ACATCTGGAG AGATCCAGTG GGTTGCCTCT GAAACCAATG ACACAAATGA CAAGAAAGAG 
GAGGAAGAAG AAGAGCTCGA CCTCAACAAA GCCTTGCAGC ATGCTGGGAA ACACATGGAG 
GACAGCATTG TGGCGTCTTA CTCAGCTCTT CTGCTTGGGT GTCTCTGTCA GGGGAGTCCG 
ATGAATGTGA AAACCGTACG GGAAAATCTT CCTAAAGGAG ATTTCTCCAT CATGACTGAA 
ATGCTGAAGA AATTCCTTAA TTTTATGAAC CTGACGTGTT CTGTTGGAAC CACAGGACAG 
AAATCTATCT CACGGGTGAT TGATTATTTG GAGCACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328854.1
CDS	268..3729
Translation	MTSRFSKTYTRKGGEASSKFDEVFSNKKATLTTKWGESTYKAHLGIKRPSFKPDVSELTKRPRFDDDDSSEDPFGFDSDDESKTVTSRDTAQSEMDSDTAGPKNEQLKTRMEKPSGGTLVTSTSSLGSSTNISRGAVGVNSPAKTSPASSYVTGTVGTWSKSNVTVSKQLSQVSSLKIPSRGISNDWDSFGEDSPPGAGEGSSTQTSNSSDELKSAEQCPAEPEPPVEQPVDFEQLPLLKPSSDRVYRRPRGKETESSAAGDTIPAVSSVSAPAPVMSSNAGVAGSSGVKPPAGRGRVRDYTVLHPSCVSVCNVTIQDSIERSVDDFVMPVQADIGEAGTFRRKADTQAPKPTRFRPTQSKSKKETKLEFFGFEENEAQDADGDATGSGSTSYKIKYFGFDDLSESDSEDDDDSAARERKKNKKAEAEALSTGTGSPQFSDSQESQQSQSSLSTDSQESLDETGATGSQKERHRKQSNKSKEIGRKIFSGPKKSPAKAVYNARHWNQPESEEVPSPASHTVAVPKPSASSSSTSSSKDTNSHKDDGVFKAPPPPPKVIKSVTIPTELNQDIVTALKCRKEHKELYTVVQHVKHFNDVVEFGENQEFTDDFEYLETGLKSTQPLNTRCLSIISLATRCAMPSFRMHLRARGKVAQVFKMLSDAQQHPNMALCTASLMYILSRDRLNMDLDRSCLELMIRLLEMEQDDSVADQLTAKEMSKVKEKIRKLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVENLNKEEDKESLVASLWGAERCLRVLESVTVHNPENQAYLIAYKDSQLIMSSARALQHCEQMIQRYSREVSSSSSSTLPQSSHSNVGKAVEDCMRAVIGVLLNLTHDNEWGSTKTGEQDGLILTALDCVLKVPRYLPQEQRFDIRVLGLGLLINLVEYSARNRECLVEMEVDMSYLMEAEAVVKIENDVKTEEQKRDQMEEEEKRKATLKSLNALAALVQLFLERERAAVLAEAQTDDLISEAPKSQADQSGEWQETSGEIQWVASETNDTNDKKEEEEEELDLNKALQHAGKHMEDSIVASYSALLLGCLCQGSPMNVKTVRENLPKGDFSIMTEMLKKFLNFMNLTCSVGTTGQKSISRVIDYLEHC

Target ORF information:

RefSeq Version	XM_021473179.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WAPL cohesin release factor a (wapla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473179.1

ORF Insert Sequence:

ATGACATCAA GATTTAGTAA AACGTACACG CGGAAGGGAG GAGAAGCCAG CTCAAAGTTT 
GACGAAGTGT TCTCTAACAA GAAAGCTACA CTCACCACCA AATGGGGAGA GTCCACCTAC 
AAGGCGCATT TGGGCATCAA ACGACCCTCA TTCAAGCCAG ATGTTTCAGA GCTGACCAAA 
CGACCCCGCT TTGATGATGA CGACAGCTCA GAGGATCCGT TTGGCTTTGA CAGCGATGAT 
GAGTCCAAAA CAGTCACATC CAGAGATACT GCTCAATCTG AGATGGACTC GGATACTGCA 
GGACCCAAGA ATGAGCAGTT AAAGACCCGG ATGGAAAAGC CCAGCGGGGG GACACTGGTG 
ACCAGTACTT CTTCCTTGGG GAGTAGTACA AACATCAGCA GAGGGGCGGT TGGCGTGAAT 
AGCCCAGCTA AAACTTCTCC AGCCTCTTCT TATGTCACTG GTACTGTAGG AACCTGGAGC 
AAATCCAATG TTACAGTAAG TAAACAGCTC TCCCAGGTCT CCAGCTTGAA GATCCCAAGT 
AGAGGCATCT CCAATGACTG GGACAGTTTT GGGGAGGACT CTCCTCCTGG AGCCGGTGAG 
GGCAGTTCTA CTCAGACTAG TAACTCTTCT GATGAGCTCA AGAGTGCAGA GCAGTGCCCT 
GCTGAACCTG AACCGCCAGT GGAGCAACCT GTTGACTTTG AGCAGCTTCC GCTTCTTAAA 
CCATCTAGCG ACAGAGTGTA CAGACGACCA CGGGGTAAAG AGACCGAAAG TTCAGCTGCT 
GGAGATACAA TTCCTGCTGT GAGTTCGGTG TCAGCTCCTG CACCAGTGAT GAGCAGCAAT 
GCAGGGGTTG CTGGGAGTTC TGGGGTGAAG CCTCCTGCAG GTCGGGGTAG AGTGAGGGAT 
TACACCGTTC TTCACCCGTC TTGTGTGTCC GTGTGTAATG TGACCATCCA GGACTCCATT 
GAAAGGAGCG TGGATGATTT TGTTATGCCA GTGCAAGCTG ACATTGGAGA AGCAGGAACC 
TTCAGGAGGA AAGCAGACAC ACAGGCTCCT AAACCTACCA GGTTTAGACC CACTCAGAGC 
AAAAGTAAAA AGGAGACAAA ACTGGAGTTT TTTGGCTTTG AAGAGAACGA GGCTCAGGAC 
GCAGATGGAG ACGCCACTGG CTCTGGCAGC ACCAGCTACA AGATCAAATA CTTTGGTTTT 
GATGATCTGA GTGAGAGTGA CAGCGAGGAT GATGATGATT CAGCAGCTAG AGAGAGGAAA 
AAGAATAAGA AAGCAGAGGC AGAGGCTTTG TCAACCGGCA CAGGAAGTCC ACAGTTCAGC 
GATTCGCAGG AAAGTCAACA GAGTCAAAGC AGCCTAAGTA CAGATTCTCA GGAGTCACTG 
GATGAAACGG GAGCTACGGG TTCACAGAAA GAACGACACA GAAAGCAAAG CAACAAGTCA 
AAGGAGATCG GCCGCAAGAT CTTCAGTGGA CCAAAAAAAT CTCCTGCTAA AGCTGTATAC 
AACGCTCGTC ACTGGAATCA GCCTGAGTCG GAGGAAGTTC CATCTCCTGC TTCTCATACT 
GTGGCTGTAC CGAAGCCAAG TGCATCCAGC AGCAGCACAA GCAGCAGCAA AGACACAAAT 
TCCCATAAAG ATGATGGAGT GTTTAAAGCT CCACCGCCTC CACCTAAAGT CATCAAGTCT 
GTCACCATCC CCACTGAGCT GAACCAGGAC ATCGTCACTG CACTGAAATG CAGGAAGGAG 
CACAAGGAGT TGTATACAGT GGTCCAACAT GTGAAGCACT TTAATGATGT GGTGGAGTTT 
GGGGAGAATC AGGAGTTCAC AGATGACTTT GAGTATCTAG AGACGGGCTT GAAGAGCACA 
CAACCTCTCA ACACACGCTG CCTTAGTATA ATCAGTCTGG CCACACGCTG TGCCATGCCA 
AGCTTCCGAA TGCATCTGAG AGCCAGAGGG AAGGTGGCGC AGGTCTTTAA GATGCTCAGT 
GATGCTCAAC AGCACCCGAA TATGGCTCTC TGCACGGCGT CTCTGATGTA TATATTGAGT 
CGAGACCGGC TGAATATGGA TCTGGATCGG TCTTGTCTGG AGCTGATGAT CCGGCTGCTG 
GAAATGGAGC AGGACGACTC TGTAGCGGAC CAGTTAACAG CTAAAGAGAT GAGCAAAGTA 
AAAGAGAAAA TCAGGAAACT CTGTGAAACA GTCCACAACA AGCATCTGGA TCTGGAGAAC 
ATCACAACTG GTCATTTAGC AATGGAAACG CTGCTGTCTC TGACGTCGAA GCGAGCTGGA 
GACTGGTTTA AGGAGGAGCT GCGTTTACTT GGGGGACTGG ACCACATCGT AGATAAAGTA 
AAAGAGTGTG TGGAGAACTT GAATAAAGAA GAAGATAAAG AGAGTCTAGT GGCGTCACTC 
TGGGGAGCAG AACGCTGCTT GCGAGTTCTG GAGAGCGTTA CAGTTCATAA CCCTGAGAAC 
CAGGCTTATC TGATTGCCTA CAAAGACTCC CAGCTCATAA TGTCCTCAGC AAGGGCCCTG 
CAGCACTGTG AGCAGATGAT CCAGCGTTAC AGCCGAGAGG TCTCTTCATC ATCGAGCTCT 
ACACTCCCGC AGAGCAGTCA CAGTAATGTG GGCAAAGCCG TGGAGGATTG CATGAGAGCC 
GTGATCGGAG TTCTGCTCAA CCTCACACAT GATAACGAGT GGGGAAGCAC TAAAACAGGA 
GAGCAGGACG GCCTGATACT GACCGCTCTG GACTGTGTTC TGAAGGTCCC GCGCTATTTG 
CCTCAGGAAC AGAGATTTGA TATCCGTGTA CTGGGTCTGG GTCTACTGAT TAATCTGGTG 
GAGTACAGTG CCAGAAACAG AGAGTGTCTG GTGGAGATGG AGGTGGACAT GAGCTACCTA 
ATGGAGGCTG AGGCTGTCGT TAAGATAGAG AACGATGTGA AGACAGAGGA ACAAAAACGC 
GATCAAATGG AAGAAGAAGA AAAGCGAAAA GCGACGCTAA AATCATTAAA TGCCTTGGCT 
GCTCTGGTTC AGCTCTTCCT CGAACGGGAG CGAGCAGCTG TGCTTGCTGA GGCACAGACT 
GATGACCTCA TAAGTGAGGC ACCCAAATCT CAGGCAGACC AGAGCGGAGA GTGGCAAGAA 
ACATCTGGAG AGATCCAGTG GGTTGCCTCT GAAACCAATG ACACAAATGA CAAGAAAGAG 
GAGGAAGAAG AAGAGCTCGA CCTCAACAAA GCCTTGCAGC ATGCTGGGAA ACACATGGAG 
GACAGCATTG TGGCGTCTTA CTCAGCTCTT CTGCTTGGGT GTCTCTGTCA GGGGAGTCCG 
ATGAATGTGA AAACCGTACG GGAAAATCTT CCTAAAGGAG ATTTCTCCAT CATGACTGAA 
ATGCTGAAGA AATTCCTTAA TTTTATGAAC CTGACGTGTT CTGTTGGAAC CACAGGACAG 
AAATCTATCT CACGGGTGAT TGATTATTTG GAGCACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPLA ( XM_683513.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_688605.5 Danio rerio WAPL cohesin release factor a (wapla), mRNA.

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CloneID	ODa22552
Clone ID Related Accession (Same CDS sequence)	XM_683513.7 , XM_021473179.1 , XM_683513.6
Accession Version	XM_683513.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	wings apart-like protein homolog
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 1, 2013 this sequence version replaced XM_683513.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCAA GATTTAGTAA AACGTACACG CGGAAGGGAG GAGAAGCCAG CTCAAAGTTT 
GACGAAGTGT TCTCTAACAA GAAAGCTACA CTCACCACCA AATGGGGAGA GTCCACCTAC 
AAGGCGCATT TGGGCATCAA ACGACCCTCA TTCAAGCCAG ATGTTTCAGA GCTGACCAAA 
CGACCCCGCT TTGATGATGA CGACAGCTCA GAGGATCCGT TTGGCTTTGA CAGCGATGAT 
GAGTCCAAAA CAGTCACATC CAGAGATACT GCTCAATCTG AGATGGACTC GGATACTGCA 
GGACCCAAGA ATGAGCAGTT AAAGACCCGG ATGGAAAAGC CCAGCGGGGG GACACTGGTG 
ACCAGTACTT CTTCCTTGGG GAGTAGTACA AACATCAGCA GAGGGGCGGT TGGCGTGAAT 
AGCCCAGCTA AAACTTCTCC AGCCTCTTCT TATGTCACTG GTACTGTAGG AACCTGGAGC 
AAATCCAATG TTACAGTAAG TAAACAGCTC TCCCAGGTCT CCAGCTTGAA GATCCCAAGT 
AGAGGCATCT CCAATGACTG GGACAGTTTT GGGGAGGACT CTCCTCCTGG AGCCGGTGAG 
GGCAGTTCTA CTCAGACTAG TAACTCTTCT GATGAGCTCA AGAGTGCAGA GCAGTGCCCT 
GCTGAACCTG AACCGCCAGT GGAGCAACCT GTTGACTTTG AGCAGCTTCC GCTTCTTAAA 
CCATCTAGCG ACAGAGTGTA CAGACGACCA CGGGGTAAAG AGACCGAAAG TTCAGCTGCT 
GGAGATACAA TTCCTGCTGT GAGTTCGGTG TCAGCTCCTG CACCAGTGAT GAGCAGCAAT 
GCAGGGGTTG CTGGGAGTTC TGGGGTGAAG CCTCCTGCAG GTCGGGGTAG AGTGAGGGAT 
TACACCGTTC TTCACCCGTC TTGTGTGTCC GTGTGTAATG TGACCATCCA GGACTCCATT 
GAAAGGAGCG TGGATGATTT TGTTATGCCA GTGCAAGCTG ACATTGGAGA AGCAGGAACC 
TTCAGGAGGA AAGCAGACAC ACAGGCTCCT AAACCTACCA GGTTTAGACC CACTCAGAGC 
AAAAGTAAAA AGGAGACAAA ACTGGAGTTT TTTGGCTTTG AAGAGAACGA GGCTCAGGAC 
GCAGATGGAG ACGCCACTGG CTCTGGCAGC ACCAGCTACA AGATCAAATA CTTTGGTTTT 
GATGATCTGA GTGAGAGTGA CAGCGAGGAT GATGATGATT CAGCAGCTAG AGAGAGGAAA 
AAGAATAAGA AAGCAGAGGC AGAGGCTTTG TCAACCGGCA CAGGAAGTCC ACAGTTCAGC 
GATTCGCAGG AAAGTCAACA GAGTCAAAGC AGCCTAAGTA CAGATTCTCA GGAGTCACTG 
GATGAAACGG GAGCTACGGG TTCACAGAAA GAACGACACA GAAAGCAAAG CAACAAGTCA 
AAGGAGATCG GCCGCAAGAT CTTCAGTGGA CCAAAAAAAT CTCCTGCTAA AGCTGTATAC 
AACGCTCGTC ACTGGAATCA GCCTGAGTCG GAGGAAGTTC CATCTCCTGC TTCTCATACT 
GTGGCTGTAC CGAAGCCAAG TGCATCCAGC AGCAGCACAA GCAGCAGCAA AGACACAAAT 
TCCCATAAAG ATGATGGAGT GTTTAAAGCT CCACCGCCTC CACCTAAAGT CATCAAGTCT 
GTCACCATCC CCACTGAGCT GAACCAGGAC ATCGTCACTG CACTGAAATG CAGGAAGGAG 
CACAAGGAGT TGTATACAGT GGTCCAACAT GTGAAGCACT TTAATGATGT GGTGGAGTTT 
GGGGAGAATC AGGAGTTCAC AGATGACTTT GAGTATCTAG AGACGGGCTT GAAGAGCACA 
CAACCTCTCA ACACACGCTG CCTTAGTATA ATCAGTCTGG CCACACGCTG TGCCATGCCA 
AGCTTCCGAA TGCATCTGAG AGCCAGAGGG AAGGTGGCGC AGGTCTTTAA GATGCTCAGT 
GATGCTCAAC AGCACCCGAA TATGGCTCTC TGCACGGCGT CTCTGATGTA TATATTGAGT 
CGAGACCGGC TGAATATGGA TCTGGATCGG TCTTGTCTGG AGCTGATGAT CCGGCTGCTG 
GAAATGGAGC AGGACGACTC TGTAGCGGAC CAGTTAACAG CTAAAGAGAT GAGCAAAGTA 
AAAGAGAAAA TCAGGAAACT CTGTGAAACA GTCCACAACA AGCATCTGGA TCTGGAGAAC 
ATCACAACTG GTCATTTAGC AATGGAAACG CTGCTGTCTC TGACGTCGAA GCGAGCTGGA 
GACTGGTTTA AGGAGGAGCT GCGTTTACTT GGGGGACTGG ACCACATCGT AGATAAAGTA 
AAAGAGTGTG TGGAGAACTT GAATAAAGAA GAAGATAAAG AGAGTCTAGT GGCGTCACTC 
TGGGGAGCAG AACGCTGCTT GCGAGTTCTG GAGAGCGTTA CAGTTCATAA CCCTGAGAAC 
CAGGCTTATC TGATTGCCTA CAAAGACTCC CAGCTCATAA TGTCCTCAGC AAGGGCCCTG 
CAGCACTGTG AGCAGATGAT CCAGCGTTAC AGCCGAGAGG TCTCTTCATC ATCGAGCTCT 
ACACTCCCGC AGAGCAGTCA CAGTAATGTG GGCAAAGCCG TGGAGGATTG CATGAGAGCC 
GTGATCGGAG TTCTGCTCAA CCTCACACAT GATAACGAGT GGGGAAGCAC TAAAACAGGA 
GAGCAGGACG GCCTGATACT GACCGCTCTG GACTGTGTTC TGAAGGTCCC GCGCTATTTG 
CCTCAGGAAC AGAGATTTGA TATCCGTGTA CTGGGTCTGG GTCTACTGAT TAATCTGGTG 
GAGTACAGTG CCAGAAACAG AGAGTGTCTG GTGGAGATGG AGGTGGACAT GAGCTACCTA 
ATGGAGGCTG AGGCTGTCGT TAAGATAGAG AACGATGTGA AGACAGAGGA ACAAAAACGC 
GATCAAATGG AAGAAGAAGA AAAGCGAAAA GCGACGCTAA AATCATTAAA TGCCTTGGCT 
GCTCTGGTTC AGCTCTTCCT CGAACGGGAG CGAGCAGCTG TGCTTGCTGA GGCACAGACT 
GATGACCTCA TAAGTGAGGC ACCCAAATCT CAGGCAGACC AGAGCGGAGA GTGGCAAGAA 
ACATCTGGAG AGATCCAGTG GGTTGCCTCT GAAACCAATG ACACAAATGA CAAGAAAGAG 
GAGGAAGAAG AAGAGCTCGA CCTCAACAAA GCCTTGCAGC ATGCTGGGAA ACACATGGAG 
GACAGCATTG TGGCGTCTTA CTCAGCTCTT CTGCTTGGGT GTCTCTGTCA GGGGAGTCCG 
ATGAATGTGA AAACCGTACG GGAAAATCTT CCTAAAGGAG ATTTCTCCAT CATGACTGAA 
ATGCTGAAGA AATTCCTTAA TTTTATGAAC CTGACGTGTT CTGTTGGAAC CACAGGACAG 
AAATCTATCT CACGGGTGAT TGATTATTTG GAGCACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688605.5
CDS	273..3734
Translation	MTSRFSKTYTRKGGEASSKFDEVFSNKKATLTTKWGESTYKAHLGIKRPSFKPDVSELTKRPRFDDDDSSEDPFGFDSDDESKTVTSRDTAQSEMDSDTAGPKNEQLKTRMEKPSGGTLVTSTSSLGSSTNISRGAVGVNSPAKTSPASSYVTGTVGTWSKSNVTVSKQLSQVSSLKIPSRGISNDWDSFGEDSPPGAGEGSSTQTSNSSDELKSAEQCPAEPEPPVEQPVDFEQLPLLKPSSDRVYRRPRGKETESSAAGDTIPAVSSVSAPAPVMSSNAGVAGSSGVKPPAGRGRVRDYTVLHPSCVSVCNVTIQDSIERSVDDFVMPVQADIGEAGTFRRKADTQAPKPTRFRPTQSKSKKETKLEFFGFEENEAQDADGDATGSGSTSYKIKYFGFDDLSESDSEDDDDSAARERKKNKKAEAEALSTGTGSPQFSDSQESQQSQSSLSTDSQESLDETGATGSQKERHRKQSNKSKEIGRKIFSGPKKSPAKAVYNARHWNQPESEEVPSPASHTVAVPKPSASSSSTSSSKDTNSHKDDGVFKAPPPPPKVIKSVTIPTELNQDIVTALKCRKEHKELYTVVQHVKHFNDVVEFGENQEFTDDFEYLETGLKSTQPLNTRCLSIISLATRCAMPSFRMHLRARGKVAQVFKMLSDAQQHPNMALCTASLMYILSRDRLNMDLDRSCLELMIRLLEMEQDDSVADQLTAKEMSKVKEKIRKLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVENLNKEEDKESLVASLWGAERCLRVLESVTVHNPENQAYLIAYKDSQLIMSSARALQHCEQMIQRYSREVSSSSSSTLPQSSHSNVGKAVEDCMRAVIGVLLNLTHDNEWGSTKTGEQDGLILTALDCVLKVPRYLPQEQRFDIRVLGLGLLINLVEYSARNRECLVEMEVDMSYLMEAEAVVKIENDVKTEEQKRDQMEEEEKRKATLKSLNALAALVQLFLERERAAVLAEAQTDDLISEAPKSQADQSGEWQETSGEIQWVASETNDTNDKKEEEEEELDLNKALQHAGKHMEDSIVASYSALLLGCLCQGSPMNVKTVRENLPKGDFSIMTEMLKKFLNFMNLTCSVGTTGQKSISRVIDYLEHC

Target ORF information:

RefSeq Version	XM_683513.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WAPL cohesin release factor a (wapla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683513.6

ORF Insert Sequence:

ATGACATCAA GATTTAGTAA AACGTACACG CGGAAGGGAG GAGAAGCCAG CTCAAAGTTT 
GACGAAGTGT TCTCTAACAA GAAAGCTACA CTCACCACCA AATGGGGAGA GTCCACCTAC 
AAGGCGCATT TGGGCATCAA ACGACCCTCA TTCAAGCCAG ATGTTTCAGA GCTGACCAAA 
CGACCCCGCT TTGATGATGA CGACAGCTCA GAGGATCCGT TTGGCTTTGA CAGCGATGAT 
GAGTCCAAAA CAGTCACATC CAGAGATACT GCTCAATCTG AGATGGACTC GGATACTGCA 
GGACCCAAGA ATGAGCAGTT AAAGACCCGG ATGGAAAAGC CCAGCGGGGG GACACTGGTG 
ACCAGTACTT CTTCCTTGGG GAGTAGTACA AACATCAGCA GAGGGGCGGT TGGCGTGAAT 
AGCCCAGCTA AAACTTCTCC AGCCTCTTCT TATGTCACTG GTACTGTAGG AACCTGGAGC 
AAATCCAATG TTACAGTAAG TAAACAGCTC TCCCAGGTCT CCAGCTTGAA GATCCCAAGT 
AGAGGCATCT CCAATGACTG GGACAGTTTT GGGGAGGACT CTCCTCCTGG AGCCGGTGAG 
GGCAGTTCTA CTCAGACTAG TAACTCTTCT GATGAGCTCA AGAGTGCAGA GCAGTGCCCT 
GCTGAACCTG AACCGCCAGT GGAGCAACCT GTTGACTTTG AGCAGCTTCC GCTTCTTAAA 
CCATCTAGCG ACAGAGTGTA CAGACGACCA CGGGGTAAAG AGACCGAAAG TTCAGCTGCT 
GGAGATACAA TTCCTGCTGT GAGTTCGGTG TCAGCTCCTG CACCAGTGAT GAGCAGCAAT 
GCAGGGGTTG CTGGGAGTTC TGGGGTGAAG CCTCCTGCAG GTCGGGGTAG AGTGAGGGAT 
TACACCGTTC TTCACCCGTC TTGTGTGTCC GTGTGTAATG TGACCATCCA GGACTCCATT 
GAAAGGAGCG TGGATGATTT TGTTATGCCA GTGCAAGCTG ACATTGGAGA AGCAGGAACC 
TTCAGGAGGA AAGCAGACAC ACAGGCTCCT AAACCTACCA GGTTTAGACC CACTCAGAGC 
AAAAGTAAAA AGGAGACAAA ACTGGAGTTT TTTGGCTTTG AAGAGAACGA GGCTCAGGAC 
GCAGATGGAG ACGCCACTGG CTCTGGCAGC ACCAGCTACA AGATCAAATA CTTTGGTTTT 
GATGATCTGA GTGAGAGTGA CAGCGAGGAT GATGATGATT CAGCAGCTAG AGAGAGGAAA 
AAGAATAAGA AAGCAGAGGC AGAGGCTTTG TCAACCGGCA CAGGAAGTCC ACAGTTCAGC 
GATTCGCAGG AAAGTCAACA GAGTCAAAGC AGCCTAAGTA CAGATTCTCA GGAGTCACTG 
GATGAAACGG GAGCTACGGG TTCACAGAAA GAACGACACA GAAAGCAAAG CAACAAGTCA 
AAGGAGATCG GCCGCAAGAT CTTCAGTGGA CCAAAAAAAT CTCCTGCTAA AGCTGTATAC 
AACGCTCGTC ACTGGAATCA GCCTGAGTCG GAGGAAGTTC CATCTCCTGC TTCTCATACT 
GTGGCTGTAC CGAAGCCAAG TGCATCCAGC AGCAGCACAA GCAGCAGCAA AGACACAAAT 
TCCCATAAAG ATGATGGAGT GTTTAAAGCT CCACCGCCTC CACCTAAAGT CATCAAGTCT 
GTCACCATCC CCACTGAGCT GAACCAGGAC ATCGTCACTG CACTGAAATG CAGGAAGGAG 
CACAAGGAGT TGTATACAGT GGTCCAACAT GTGAAGCACT TTAATGATGT GGTGGAGTTT 
GGGGAGAATC AGGAGTTCAC AGATGACTTT GAGTATCTAG AGACGGGCTT GAAGAGCACA 
CAACCTCTCA ACACACGCTG CCTTAGTATA ATCAGTCTGG CCACACGCTG TGCCATGCCA 
AGCTTCCGAA TGCATCTGAG AGCCAGAGGG AAGGTGGCGC AGGTCTTTAA GATGCTCAGT 
GATGCTCAAC AGCACCCGAA TATGGCTCTC TGCACGGCGT CTCTGATGTA TATATTGAGT 
CGAGACCGGC TGAATATGGA TCTGGATCGG TCTTGTCTGG AGCTGATGAT CCGGCTGCTG 
GAAATGGAGC AGGACGACTC TGTAGCGGAC CAGTTAACAG CTAAAGAGAT GAGCAAAGTA 
AAAGAGAAAA TCAGGAAACT CTGTGAAACA GTCCACAACA AGCATCTGGA TCTGGAGAAC 
ATCACAACTG GTCATTTAGC AATGGAAACG CTGCTGTCTC TGACGTCGAA GCGAGCTGGA 
GACTGGTTTA AGGAGGAGCT GCGTTTACTT GGGGGACTGG ACCACATCGT AGATAAAGTA 
AAAGAGTGTG TGGAGAACTT GAATAAAGAA GAAGATAAAG AGAGTCTAGT GGCGTCACTC 
TGGGGAGCAG AACGCTGCTT GCGAGTTCTG GAGAGCGTTA CAGTTCATAA CCCTGAGAAC 
CAGGCTTATC TGATTGCCTA CAAAGACTCC CAGCTCATAA TGTCCTCAGC AAGGGCCCTG 
CAGCACTGTG AGCAGATGAT CCAGCGTTAC AGCCGAGAGG TCTCTTCATC ATCGAGCTCT 
ACACTCCCGC AGAGCAGTCA CAGTAATGTG GGCAAAGCCG TGGAGGATTG CATGAGAGCC 
GTGATCGGAG TTCTGCTCAA CCTCACACAT GATAACGAGT GGGGAAGCAC TAAAACAGGA 
GAGCAGGACG GCCTGATACT GACCGCTCTG GACTGTGTTC TGAAGGTCCC GCGCTATTTG 
CCTCAGGAAC AGAGATTTGA TATCCGTGTA CTGGGTCTGG GTCTACTGAT TAATCTGGTG 
GAGTACAGTG CCAGAAACAG AGAGTGTCTG GTGGAGATGG AGGTGGACAT GAGCTACCTA 
ATGGAGGCTG AGGCTGTCGT TAAGATAGAG AACGATGTGA AGACAGAGGA ACAAAAACGC 
GATCAAATGG AAGAAGAAGA AAAGCGAAAA GCGACGCTAA AATCATTAAA TGCCTTGGCT 
GCTCTGGTTC AGCTCTTCCT CGAACGGGAG CGAGCAGCTG TGCTTGCTGA GGCACAGACT 
GATGACCTCA TAAGTGAGGC ACCCAAATCT CAGGCAGACC AGAGCGGAGA GTGGCAAGAA 
ACATCTGGAG AGATCCAGTG GGTTGCCTCT GAAACCAATG ACACAAATGA CAAGAAAGAG 
GAGGAAGAAG AAGAGCTCGA CCTCAACAAA GCCTTGCAGC ATGCTGGGAA ACACATGGAG 
GACAGCATTG TGGCGTCTTA CTCAGCTCTT CTGCTTGGGT GTCTCTGTCA GGGGAGTCCG 
ATGAATGTGA AAACCGTACG GGAAAATCTT CCTAAAGGAG ATTTCTCCAT CATGACTGAA 
ATGCTGAAGA AATTCCTTAA TTTTATGAAC CTGACGTGTT CTGTTGGAAC CACAGGACAG 
AAATCTATCT CACGGGTGAT TGATTATTTG GAGCACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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WAPLA cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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