WAPL cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	WAPL
Entrez Gene ID	612854
Full Name	WAPL cohesin release factor
Synonyms	WAPAL
General protein information	Preferred Names WAPL cohesin release factor Names wings apart-like protein homolog wings apart-like homolog
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_859725.4	XP_864818.3	wings apart-like protein homolog isoform X2
XM_859741.4	XP_864834.3	wings apart-like protein homolog isoform X1
XM_859725.5	XP_864818.3	wings apart-like protein homolog isoform X2
XM_859741.5	XP_864834.3	wings apart-like protein homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	WAPAL	NP_055860.1
Pan troglodytes (chimpanzee)	WAPAL	XP_003312721.1
Macaca mulatta (Rhesus monkey)	WAPAL	XP_002805691.1
Canis lupus familiaris (dog)	WAPAL	XP_864834.3
Mus musculus (house mouse)	Wapal	NP_001004436.2
Gallus gallus (chicken)	WAPAL	NP_001186440.1
Danio rerio (zebrafish)	wapala	XP_688605.5
Xenopus tropicalis (tropical clawed frog)	wapal	XP_002937603.2
Bos taurus (cattle)	WAPAL	XP_005226580.1
Caenorhabditis elegans (roundworm)	wapl-1	NP_500567.1
Rattus norvegicus (Norway rat)	Wapal	XP_006252785.1

Gene Ontology

The following WAPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WAPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe05140	XM_859741.5 Latest version!	Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCe05141	XM_859725.5 Latest version!	Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCe05141	XM_859725.4	Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCe05140	XM_859741.4	Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WAPL ( XM_859741.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_864834.3 Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe05140
Clone ID Related Accession (Same CDS sequence)	XM_859741.4 , XM_859741.5
Accession Version	XM_859741.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	wings apart-like protein homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_859741.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT ACTTTTTTTT CCCCCCCTAC AGAGAATATG AAACAGGTGT CAAAATGACA 
TCCAGATTTG GGAAAACATA CAGTAGGAAA GGAGGAAATG GCAGTTCAAA ATTCGATGAA 
GTCTTTTCCA ATAAACGGAC CACCCTTAGC ACAAAATGGG GAGAGACCAC ATTTATGGCT 
AAATTAGGGC AGAAGAGGCC CAATTTCAAA CCAGATATCC AAGAAATTCC GAAGAAACCT 
AAGGTGGAAG AAGAAAATAC TGGAGATCCT TTTGGATTTG ATAGTGATGA TGAGTCTCTA 
CCAGTTTCTT CAAAGAATTT AGCCCAGGGT AAGGGTTCCT CTTACTCAGA ATCTAGTGAA 
GCTGCTCAGC TGGAAGAGGT CACTTCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGCA 
GTGGGCGAAG ACAGTGTTGT ATCTGATAAA TGCTTACTTT TGGAAGAAAC TTTGGTTGGG 
AAAGAAAAGA GCACAAACCG AATTTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA 
TTAGCGACTT CAGATAAAGT GGAGAATTTT AGTGAAGAAT ATGAAAAGAA TAGTCACCAT 
ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACCCAGTG CAGAAACAAC AGTAGCTTCT 
GTATACAAAG CTGATGAAAT CAAGGAAACA AATGACACTT GGAACTCTCA ACTTGGAAAA 
AGGTCAGAAT CTCCATCAGA AACATCTCCC ATTAAGGGAT CTGTAAGAAC TGGTTTGTAT 
GAATGGGATA ATGATTTTGA AGATATCAGA TCAGAAGATT GTATTTTAAG TTTGGATAGT 
GATCCTCTTT TGGAGATGAA GGATGAGGAT TTTAAAAACC GGTTGGAAAA TCTAAATGAA 
GCCATTGAGG AAGACATCAT GCAAAGTGTT CTTAGGCCAA GCAACTGTAG GACGTACTGT 
AGGGCCAATA AAGCAAAATC CTCACAAGGA GCTTCAAATT TTGATAAGCT AATGGACGGA 
ACCAGTCAGG CCTTAGCCAA AGCAAACAGT GAATCAAGTA AGGATGGCCT GAATCAGGCA 
AAGAAAGGCA GTGTAAGTTC TGGGACCAGT TTTAGAGGGA CAGTTGGACG GACTAGAGAT 
TACACTGTTT TACATCCATC TTGCTTGTCA GTTTGTAATG TTACCATACA GGATACTATG 
GAACGGAGCA TGGATGAATT CACTGCATCA ACCCCTGCAG ATTTGGGCGA AGCTGGCCGC 
CTAAGAAAAA AGGCAGATAT TGCGACTTCT AAGACCACTA CTAGATTTCG ACCTAGTAAT 
ACTAAATCTA AAAAGGATGT TAAACTTGAA TTTTTTGGTT TTGAAGATCA TGATGAGACA 
GGAGGTGATG AAGGAGGTTC TGGGAGTTCT AATTACAAAA TTAAGTATTT TGGTTTTGAT 
GATCTCAGTG AAAGTGAAGA TGATGATGAA GATGACTGTC AAGTGGAAAG AAAGACAAGC 
AAAAAAAGAA CTAAAACAGC TCCATCCCCC TCCTTGCAGC CTCCTCCTGA AAGCATTGAT 
AATTCCCAGG ACAGTCAGTC TAGTGCTAAC AATGCAGAAA ACTTGAATTT TACAGAGGAC 
TTGCCTGGTG TGCCTGAAAG TGTGAAGAAG CCCCCAAATA AACAAGGAGA TAAATCAAAA 
GAAAACACCA GAAAGATTTT TAGTGGTCCC AAACGGTCTC CTACAAAAGC TGTATATAAT 
GCTAGACATT GGAACCATCC AGACTCTGAA GAGCTGCCTG GGCCACCAAT AGTAAAACCT 
CAGAGTGCCA CAGTGAGGCT GTCTTCAAAG GAACCAAATC AAAAAGATGA TGGAGTTTTT 
AAGGCTCCTG CACCACCACC CAAAGTGATA AAAACTGTGA CAATACCTAC TCAGCCCTAC 
CAAGATATAG TTACTGCACT GAAATGCAGA AAAGAAGACA AAGAATTGTA TACTGTTGTT 
CAGCATGTGA AGCACTTCAA TGATGTGGTG GAATTTGGTG AGAATCAAGA ATTCACTGAT 
GACATTGAGT ACCTGTTAAG TGGCTTAAAG AGCACTCAGC CTCTAAACAC ACGTTGCCTT 
AGTGTTATTA GCTTGGCTAC TAAATGTGCC ATGCCCAGTT TTCGAATGCA CCTGAGAGCA 
CATGGAATGG TAGCAATGGT CTTTAAAACC TTGGATGATT CCCAGCACCA TCAGAATCTG 
TCCCTTTGTA CAGCTGCCCT CATGTACATA CTGAGTAGAG ATCGTCTGAA CATGGATCTT 
GATAGAGCTA GCCTAGATCT AATGATTCGA CTTCTAGAAC TGGAACAAGA TGCTTCTTCA 
GCTAAGCTAC TGAATGAAAA AGACATGAAC AAAATCAAAG AAAAAATTCG AAGGCTCTGT 
GAAACTGTAC ACAACAAGCA TCTTGATCTA GAAAATATAA CGACTGGGCA TTTAGCTATG 
GAGACCTTGC TGTCCCTTAC TTCTAAACGA GCAGGAGACT GGTTTAAAGA AGAACTCCGG 
CTTTTGGGTG GTCTGGATCA TATTGTAGAT AAAGTGAAAG AATGTGTGGA TCATTTAAGT 
AGAGATGAAG ATGAAGAGAA ACTGGTAGCC TCATTATGGG GAGCAGAGAG ATGTTTACGA 
GTTTTAGAAA GTGTAACAGT GCATAATCCA GAGAATCAAA GCTACTTGAT AGCATATAAA 
GATTCACAAC TCATTGTTTC TTCAGCTAAA GCATTACAGC ATTGTGAAGG ATTGATTCAG 
CAGTATAACC GTGCTGAGAA CAGTATATGC TTACCTGACA GTAAGCCTCT GCCTCACCAG 
AATGTAACCA ACCATGTGGG CAAAGCCGTA GAAGACTGCA TGAGGGCCAT CATTGGGGTA 
TTGCTCAATT TAACTAATGA TAATGAGTGG GGCAGCACCA AAACGGGAGA ACAGGATGGC 
TTGATTGGCA CAGCAATGAA CTGTGTGCTT CAGGTTCCAA AGTACCTACC TCAGGAGCAG 
AGATTTGATA TTCGAGTGCT GGGCTTAGGT CTACTGATAA ATCTAGTGGA GTATAGTGCT 
CGGAATCGGC ACTGTCTTGT CAACATGGAA ACATCATGTT CTTTTGATTC TTCCCTCTGT 
AGTGGAGAAG GAGATGATAG TTTAAGGATA GCTGGACAAA TTCATGCCGT TCAGGCCTTA 
GTGCAGCTAT TCCTTGAGCG AGAACGAGCA GCCCAGTTGG CAGAAAGTAA AACAGATGAA 
TTGATCAAAG ATGCTCCCAC CACTCAGCAT GATAAGAGTG GAGAGTGGCA AGAGACAAGT 
GGAGAAATAC AGTGGGTATC AACTGAAAAG ACTGATGGTA CAGAAGAGAA ACATAAGAAG 
GAAGAGGACG ATGAAGAACT TGACCTCAAT AAAGCCCTTC AGCATGCTGG CAAACACATG 
GAGGATTGCA TTGTGGCCTC ATATACAGCA CTACTTCTCG GGTGTCTCTG CCAGGAGAGT 
CCAATCAATG TGACCACTGT GCGAGAATAT CTGCCAGAAG GCGACTTTTC AATAATGACA 
GAGATGCTCA AAAAATTCCT GAGCTTCATG AATCTTACTT GTGCTGTTGG AACCACAGGC 
CAGAAATCTA TCTCTAGAGT GATTGAATAC TTGGAACATT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_864834.3
CDS	331..3975
Translation	MNSYFFFPPYREYETGVKMTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEENTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVLEANSKISHAVGEDSVVSDKCLLLEETLVGKEKSTNRILEDDASRSSCAKLATSDKVENFSEEYEKNSHHIYKNAEDSTKKPSAETTVASVYKADEIKETNDTWNSQLGKRSESPSETSPIKGSVRTGLYEWDNDFEDIRSEDCILSLDSDPLLEMKDEDFKNRLENLNEAIEEDIMQSVLRPSNCRTYCRANKAKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGSVSSGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDEDDCQVERKTSKKRTKTAPSPSLQPPPESIDNSQDSQSSANNAENLNFTEDLPGVPESVKKPPNKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPIVKPQSATVRLSSKEPNQKDDGVFKAPAPPPKVIKTVTIPTQPYQDIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEGLIQQYNRAENSICLPDSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTAMNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSLCSGEGDDSLRIAGQIHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEDDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_859741.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_859741.4

ORF Insert Sequence:

ATGAATTCTT ACTTTTTTTT CCCCCCCTAC AGAGAATATG AAACAGGTGT CAAAATGACA 
TCCAGATTTG GGAAAACATA CAGTAGGAAA GGAGGAAATG GCAGTTCAAA ATTCGATGAA 
GTCTTTTCCA ATAAACGGAC CACCCTTAGC ACAAAATGGG GAGAGACCAC ATTTATGGCT 
AAATTAGGGC AGAAGAGGCC CAATTTCAAA CCAGATATCC AAGAAATTCC GAAGAAACCT 
AAGGTGGAAG AAGAAAATAC TGGAGATCCT TTTGGATTTG ATAGTGATGA TGAGTCTCTA 
CCAGTTTCTT CAAAGAATTT AGCCCAGGGT AAGGGTTCCT CTTACTCAGA ATCTAGTGAA 
GCTGCTCAGC TGGAAGAGGT CACTTCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGCA 
GTGGGCGAAG ACAGTGTTGT ATCTGATAAA TGCTTACTTT TGGAAGAAAC TTTGGTTGGG 
AAAGAAAAGA GCACAAACCG AATTTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA 
TTAGCGACTT CAGATAAAGT GGAGAATTTT AGTGAAGAAT ATGAAAAGAA TAGTCACCAT 
ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACCCAGTG CAGAAACAAC AGTAGCTTCT 
GTATACAAAG CTGATGAAAT CAAGGAAACA AATGACACTT GGAACTCTCA ACTTGGAAAA 
AGGTCAGAAT CTCCATCAGA AACATCTCCC ATTAAGGGAT CTGTAAGAAC TGGTTTGTAT 
GAATGGGATA ATGATTTTGA AGATATCAGA TCAGAAGATT GTATTTTAAG TTTGGATAGT 
GATCCTCTTT TGGAGATGAA GGATGAGGAT TTTAAAAACC GGTTGGAAAA TCTAAATGAA 
GCCATTGAGG AAGACATCAT GCAAAGTGTT CTTAGGCCAA GCAACTGTAG GACGTACTGT 
AGGGCCAATA AAGCAAAATC CTCACAAGGA GCTTCAAATT TTGATAAGCT AATGGACGGA 
ACCAGTCAGG CCTTAGCCAA AGCAAACAGT GAATCAAGTA AGGATGGCCT GAATCAGGCA 
AAGAAAGGCA GTGTAAGTTC TGGGACCAGT TTTAGAGGGA CAGTTGGACG GACTAGAGAT 
TACACTGTTT TACATCCATC TTGCTTGTCA GTTTGTAATG TTACCATACA GGATACTATG 
GAACGGAGCA TGGATGAATT CACTGCATCA ACCCCTGCAG ATTTGGGCGA AGCTGGCCGC 
CTAAGAAAAA AGGCAGATAT TGCGACTTCT AAGACCACTA CTAGATTTCG ACCTAGTAAT 
ACTAAATCTA AAAAGGATGT TAAACTTGAA TTTTTTGGTT TTGAAGATCA TGATGAGACA 
GGAGGTGATG AAGGAGGTTC TGGGAGTTCT AATTACAAAA TTAAGTATTT TGGTTTTGAT 
GATCTCAGTG AAAGTGAAGA TGATGATGAA GATGACTGTC AAGTGGAAAG AAAGACAAGC 
AAAAAAAGAA CTAAAACAGC TCCATCCCCC TCCTTGCAGC CTCCTCCTGA AAGCATTGAT 
AATTCCCAGG ACAGTCAGTC TAGTGCTAAC AATGCAGAAA ACTTGAATTT TACAGAGGAC 
TTGCCTGGTG TGCCTGAAAG TGTGAAGAAG CCCCCAAATA AACAAGGAGA TAAATCAAAA 
GAAAACACCA GAAAGATTTT TAGTGGTCCC AAACGGTCTC CTACAAAAGC TGTATATAAT 
GCTAGACATT GGAACCATCC AGACTCTGAA GAGCTGCCTG GGCCACCAAT AGTAAAACCT 
CAGAGTGCCA CAGTGAGGCT GTCTTCAAAG GAACCAAATC AAAAAGATGA TGGAGTTTTT 
AAGGCTCCTG CACCACCACC CAAAGTGATA AAAACTGTGA CAATACCTAC TCAGCCCTAC 
CAAGATATAG TTACTGCACT GAAATGCAGA AAAGAAGACA AAGAATTGTA TACTGTTGTT 
CAGCATGTGA AGCACTTCAA TGATGTGGTG GAATTTGGTG AGAATCAAGA ATTCACTGAT 
GACATTGAGT ACCTGTTAAG TGGCTTAAAG AGCACTCAGC CTCTAAACAC ACGTTGCCTT 
AGTGTTATTA GCTTGGCTAC TAAATGTGCC ATGCCCAGTT TTCGAATGCA CCTGAGAGCA 
CATGGAATGG TAGCAATGGT CTTTAAAACC TTGGATGATT CCCAGCACCA TCAGAATCTG 
TCCCTTTGTA CAGCTGCCCT CATGTACATA CTGAGTAGAG ATCGTCTGAA CATGGATCTT 
GATAGAGCTA GCCTAGATCT AATGATTCGA CTTCTAGAAC TGGAACAAGA TGCTTCTTCA 
GCTAAGCTAC TGAATGAAAA AGACATGAAC AAAATCAAAG AAAAAATTCG AAGGCTCTGT 
GAAACTGTAC ACAACAAGCA TCTTGATCTA GAAAATATAA CGACTGGGCA TTTAGCTATG 
GAGACCTTGC TGTCCCTTAC TTCTAAACGA GCAGGAGACT GGTTTAAAGA AGAACTCCGG 
CTTTTGGGTG GTCTGGATCA TATTGTAGAT AAAGTGAAAG AATGTGTGGA TCATTTAAGT 
AGAGATGAAG ATGAAGAGAA ACTGGTAGCC TCATTATGGG GAGCAGAGAG ATGTTTACGA 
GTTTTAGAAA GTGTAACAGT GCATAATCCA GAGAATCAAA GCTACTTGAT AGCATATAAA 
GATTCACAAC TCATTGTTTC TTCAGCTAAA GCATTACAGC ATTGTGAAGG ATTGATTCAG 
CAGTATAACC GTGCTGAGAA CAGTATATGC TTACCTGACA GTAAGCCTCT GCCTCACCAG 
AATGTAACCA ACCATGTGGG CAAAGCCGTA GAAGACTGCA TGAGGGCCAT CATTGGGGTA 
TTGCTCAATT TAACTAATGA TAATGAGTGG GGCAGCACCA AAACGGGAGA ACAGGATGGC 
TTGATTGGCA CAGCAATGAA CTGTGTGCTT CAGGTTCCAA AGTACCTACC TCAGGAGCAG 
AGATTTGATA TTCGAGTGCT GGGCTTAGGT CTACTGATAA ATCTAGTGGA GTATAGTGCT 
CGGAATCGGC ACTGTCTTGT CAACATGGAA ACATCATGTT CTTTTGATTC TTCCCTCTGT 
AGTGGAGAAG GAGATGATAG TTTAAGGATA GCTGGACAAA TTCATGCCGT TCAGGCCTTA 
GTGCAGCTAT TCCTTGAGCG AGAACGAGCA GCCCAGTTGG CAGAAAGTAA AACAGATGAA 
TTGATCAAAG ATGCTCCCAC CACTCAGCAT GATAAGAGTG GAGAGTGGCA AGAGACAAGT 
GGAGAAATAC AGTGGGTATC AACTGAAAAG ACTGATGGTA CAGAAGAGAA ACATAAGAAG 
GAAGAGGACG ATGAAGAACT TGACCTCAAT AAAGCCCTTC AGCATGCTGG CAAACACATG 
GAGGATTGCA TTGTGGCCTC ATATACAGCA CTACTTCTCG GGTGTCTCTG CCAGGAGAGT 
CCAATCAATG TGACCACTGT GCGAGAATAT CTGCCAGAAG GCGACTTTTC AATAATGACA 
GAGATGCTCA AAAAATTCCT GAGCTTCATG AATCTTACTT GTGCTGTTGG AACCACAGGC 
CAGAAATCTA TCTCTAGAGT GATTGAATAC TTGGAACATT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( XM_859741.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_864834.3 Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe05140
Clone ID Related Accession (Same CDS sequence)	XM_859741.4 , XM_859741.5
Accession Version	XM_859741.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	wings apart-like protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_859741.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT ACTTTTTTTT CCCCCCCTAC AGAGAATATG AAACAGGTGT CAAAATGACA 
TCCAGATTTG GGAAAACATA CAGTAGGAAA GGAGGAAATG GCAGTTCAAA ATTCGATGAA 
GTCTTTTCCA ATAAACGGAC CACCCTTAGC ACAAAATGGG GAGAGACCAC ATTTATGGCT 
AAATTAGGGC AGAAGAGGCC CAATTTCAAA CCAGATATCC AAGAAATTCC GAAGAAACCT 
AAGGTGGAAG AAGAAAATAC TGGAGATCCT TTTGGATTTG ATAGTGATGA TGAGTCTCTA 
CCAGTTTCTT CAAAGAATTT AGCCCAGGGT AAGGGTTCCT CTTACTCAGA ATCTAGTGAA 
GCTGCTCAGC TGGAAGAGGT CACTTCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGCA 
GTGGGCGAAG ACAGTGTTGT ATCTGATAAA TGCTTACTTT TGGAAGAAAC TTTGGTTGGG 
AAAGAAAAGA GCACAAACCG AATTTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA 
TTAGCGACTT CAGATAAAGT GGAGAATTTT AGTGAAGAAT ATGAAAAGAA TAGTCACCAT 
ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACCCAGTG CAGAAACAAC AGTAGCTTCT 
GTATACAAAG CTGATGAAAT CAAGGAAACA AATGACACTT GGAACTCTCA ACTTGGAAAA 
AGGTCAGAAT CTCCATCAGA AACATCTCCC ATTAAGGGAT CTGTAAGAAC TGGTTTGTAT 
GAATGGGATA ATGATTTTGA AGATATCAGA TCAGAAGATT GTATTTTAAG TTTGGATAGT 
GATCCTCTTT TGGAGATGAA GGATGAGGAT TTTAAAAACC GGTTGGAAAA TCTAAATGAA 
GCCATTGAGG AAGACATCAT GCAAAGTGTT CTTAGGCCAA GCAACTGTAG GACGTACTGT 
AGGGCCAATA AAGCAAAATC CTCACAAGGA GCTTCAAATT TTGATAAGCT AATGGACGGA 
ACCAGTCAGG CCTTAGCCAA AGCAAACAGT GAATCAAGTA AGGATGGCCT GAATCAGGCA 
AAGAAAGGCA GTGTAAGTTC TGGGACCAGT TTTAGAGGGA CAGTTGGACG GACTAGAGAT 
TACACTGTTT TACATCCATC TTGCTTGTCA GTTTGTAATG TTACCATACA GGATACTATG 
GAACGGAGCA TGGATGAATT CACTGCATCA ACCCCTGCAG ATTTGGGCGA AGCTGGCCGC 
CTAAGAAAAA AGGCAGATAT TGCGACTTCT AAGACCACTA CTAGATTTCG ACCTAGTAAT 
ACTAAATCTA AAAAGGATGT TAAACTTGAA TTTTTTGGTT TTGAAGATCA TGATGAGACA 
GGAGGTGATG AAGGAGGTTC TGGGAGTTCT AATTACAAAA TTAAGTATTT TGGTTTTGAT 
GATCTCAGTG AAAGTGAAGA TGATGATGAA GATGACTGTC AAGTGGAAAG AAAGACAAGC 
AAAAAAAGAA CTAAAACAGC TCCATCCCCC TCCTTGCAGC CTCCTCCTGA AAGCATTGAT 
AATTCCCAGG ACAGTCAGTC TAGTGCTAAC AATGCAGAAA ACTTGAATTT TACAGAGGAC 
TTGCCTGGTG TGCCTGAAAG TGTGAAGAAG CCCCCAAATA AACAAGGAGA TAAATCAAAA 
GAAAACACCA GAAAGATTTT TAGTGGTCCC AAACGGTCTC CTACAAAAGC TGTATATAAT 
GCTAGACATT GGAACCATCC AGACTCTGAA GAGCTGCCTG GGCCACCAAT AGTAAAACCT 
CAGAGTGCCA CAGTGAGGCT GTCTTCAAAG GAACCAAATC AAAAAGATGA TGGAGTTTTT 
AAGGCTCCTG CACCACCACC CAAAGTGATA AAAACTGTGA CAATACCTAC TCAGCCCTAC 
CAAGATATAG TTACTGCACT GAAATGCAGA AAAGAAGACA AAGAATTGTA TACTGTTGTT 
CAGCATGTGA AGCACTTCAA TGATGTGGTG GAATTTGGTG AGAATCAAGA ATTCACTGAT 
GACATTGAGT ACCTGTTAAG TGGCTTAAAG AGCACTCAGC CTCTAAACAC ACGTTGCCTT 
AGTGTTATTA GCTTGGCTAC TAAATGTGCC ATGCCCAGTT TTCGAATGCA CCTGAGAGCA 
CATGGAATGG TAGCAATGGT CTTTAAAACC TTGGATGATT CCCAGCACCA TCAGAATCTG 
TCCCTTTGTA CAGCTGCCCT CATGTACATA CTGAGTAGAG ATCGTCTGAA CATGGATCTT 
GATAGAGCTA GCCTAGATCT AATGATTCGA CTTCTAGAAC TGGAACAAGA TGCTTCTTCA 
GCTAAGCTAC TGAATGAAAA AGACATGAAC AAAATCAAAG AAAAAATTCG AAGGCTCTGT 
GAAACTGTAC ACAACAAGCA TCTTGATCTA GAAAATATAA CGACTGGGCA TTTAGCTATG 
GAGACCTTGC TGTCCCTTAC TTCTAAACGA GCAGGAGACT GGTTTAAAGA AGAACTCCGG 
CTTTTGGGTG GTCTGGATCA TATTGTAGAT AAAGTGAAAG AATGTGTGGA TCATTTAAGT 
AGAGATGAAG ATGAAGAGAA ACTGGTAGCC TCATTATGGG GAGCAGAGAG ATGTTTACGA 
GTTTTAGAAA GTGTAACAGT GCATAATCCA GAGAATCAAA GCTACTTGAT AGCATATAAA 
GATTCACAAC TCATTGTTTC TTCAGCTAAA GCATTACAGC ATTGTGAAGG ATTGATTCAG 
CAGTATAACC GTGCTGAGAA CAGTATATGC TTACCTGACA GTAAGCCTCT GCCTCACCAG 
AATGTAACCA ACCATGTGGG CAAAGCCGTA GAAGACTGCA TGAGGGCCAT CATTGGGGTA 
TTGCTCAATT TAACTAATGA TAATGAGTGG GGCAGCACCA AAACGGGAGA ACAGGATGGC 
TTGATTGGCA CAGCAATGAA CTGTGTGCTT CAGGTTCCAA AGTACCTACC TCAGGAGCAG 
AGATTTGATA TTCGAGTGCT GGGCTTAGGT CTACTGATAA ATCTAGTGGA GTATAGTGCT 
CGGAATCGGC ACTGTCTTGT CAACATGGAA ACATCATGTT CTTTTGATTC TTCCCTCTGT 
AGTGGAGAAG GAGATGATAG TTTAAGGATA GCTGGACAAA TTCATGCCGT TCAGGCCTTA 
GTGCAGCTAT TCCTTGAGCG AGAACGAGCA GCCCAGTTGG CAGAAAGTAA AACAGATGAA 
TTGATCAAAG ATGCTCCCAC CACTCAGCAT GATAAGAGTG GAGAGTGGCA AGAGACAAGT 
GGAGAAATAC AGTGGGTATC AACTGAAAAG ACTGATGGTA CAGAAGAGAA ACATAAGAAG 
GAAGAGGACG ATGAAGAACT TGACCTCAAT AAAGCCCTTC AGCATGCTGG CAAACACATG 
GAGGATTGCA TTGTGGCCTC ATATACAGCA CTACTTCTCG GGTGTCTCTG CCAGGAGAGT 
CCAATCAATG TGACCACTGT GCGAGAATAT CTGCCAGAAG GCGACTTTTC AATAATGACA 
GAGATGCTCA AAAAATTCCT GAGCTTCATG AATCTTACTT GTGCTGTTGG AACCACAGGC 
CAGAAATCTA TCTCTAGAGT GATTGAATAC TTGGAACATT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_864834.3
CDS	1162..4806
Translation	MNSYFFFPPYREYETGVKMTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEENTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVLEANSKISHAVGEDSVVSDKCLLLEETLVGKEKSTNRILEDDASRSSCAKLATSDKVENFSEEYEKNSHHIYKNAEDSTKKPSAETTVASVYKADEIKETNDTWNSQLGKRSESPSETSPIKGSVRTGLYEWDNDFEDIRSEDCILSLDSDPLLEMKDEDFKNRLENLNEAIEEDIMQSVLRPSNCRTYCRANKAKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGSVSSGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDEDDCQVERKTSKKRTKTAPSPSLQPPPESIDNSQDSQSSANNAENLNFTEDLPGVPESVKKPPNKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPIVKPQSATVRLSSKEPNQKDDGVFKAPAPPPKVIKTVTIPTQPYQDIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEGLIQQYNRAENSICLPDSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTAMNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSLCSGEGDDSLRIAGQIHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEDDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_859741.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_859741.5

ORF Insert Sequence:

ATGAATTCTT ACTTTTTTTT CCCCCCCTAC AGAGAATATG AAACAGGTGT CAAAATGACA 
TCCAGATTTG GGAAAACATA CAGTAGGAAA GGAGGAAATG GCAGTTCAAA ATTCGATGAA 
GTCTTTTCCA ATAAACGGAC CACCCTTAGC ACAAAATGGG GAGAGACCAC ATTTATGGCT 
AAATTAGGGC AGAAGAGGCC CAATTTCAAA CCAGATATCC AAGAAATTCC GAAGAAACCT 
AAGGTGGAAG AAGAAAATAC TGGAGATCCT TTTGGATTTG ATAGTGATGA TGAGTCTCTA 
CCAGTTTCTT CAAAGAATTT AGCCCAGGGT AAGGGTTCCT CTTACTCAGA ATCTAGTGAA 
GCTGCTCAGC TGGAAGAGGT CACTTCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGCA 
GTGGGCGAAG ACAGTGTTGT ATCTGATAAA TGCTTACTTT TGGAAGAAAC TTTGGTTGGG 
AAAGAAAAGA GCACAAACCG AATTTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA 
TTAGCGACTT CAGATAAAGT GGAGAATTTT AGTGAAGAAT ATGAAAAGAA TAGTCACCAT 
ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACCCAGTG CAGAAACAAC AGTAGCTTCT 
GTATACAAAG CTGATGAAAT CAAGGAAACA AATGACACTT GGAACTCTCA ACTTGGAAAA 
AGGTCAGAAT CTCCATCAGA AACATCTCCC ATTAAGGGAT CTGTAAGAAC TGGTTTGTAT 
GAATGGGATA ATGATTTTGA AGATATCAGA TCAGAAGATT GTATTTTAAG TTTGGATAGT 
GATCCTCTTT TGGAGATGAA GGATGAGGAT TTTAAAAACC GGTTGGAAAA TCTAAATGAA 
GCCATTGAGG AAGACATCAT GCAAAGTGTT CTTAGGCCAA GCAACTGTAG GACGTACTGT 
AGGGCCAATA AAGCAAAATC CTCACAAGGA GCTTCAAATT TTGATAAGCT AATGGACGGA 
ACCAGTCAGG CCTTAGCCAA AGCAAACAGT GAATCAAGTA AGGATGGCCT GAATCAGGCA 
AAGAAAGGCA GTGTAAGTTC TGGGACCAGT TTTAGAGGGA CAGTTGGACG GACTAGAGAT 
TACACTGTTT TACATCCATC TTGCTTGTCA GTTTGTAATG TTACCATACA GGATACTATG 
GAACGGAGCA TGGATGAATT CACTGCATCA ACCCCTGCAG ATTTGGGCGA AGCTGGCCGC 
CTAAGAAAAA AGGCAGATAT TGCGACTTCT AAGACCACTA CTAGATTTCG ACCTAGTAAT 
ACTAAATCTA AAAAGGATGT TAAACTTGAA TTTTTTGGTT TTGAAGATCA TGATGAGACA 
GGAGGTGATG AAGGAGGTTC TGGGAGTTCT AATTACAAAA TTAAGTATTT TGGTTTTGAT 
GATCTCAGTG AAAGTGAAGA TGATGATGAA GATGACTGTC AAGTGGAAAG AAAGACAAGC 
AAAAAAAGAA CTAAAACAGC TCCATCCCCC TCCTTGCAGC CTCCTCCTGA AAGCATTGAT 
AATTCCCAGG ACAGTCAGTC TAGTGCTAAC AATGCAGAAA ACTTGAATTT TACAGAGGAC 
TTGCCTGGTG TGCCTGAAAG TGTGAAGAAG CCCCCAAATA AACAAGGAGA TAAATCAAAA 
GAAAACACCA GAAAGATTTT TAGTGGTCCC AAACGGTCTC CTACAAAAGC TGTATATAAT 
GCTAGACATT GGAACCATCC AGACTCTGAA GAGCTGCCTG GGCCACCAAT AGTAAAACCT 
CAGAGTGCCA CAGTGAGGCT GTCTTCAAAG GAACCAAATC AAAAAGATGA TGGAGTTTTT 
AAGGCTCCTG CACCACCACC CAAAGTGATA AAAACTGTGA CAATACCTAC TCAGCCCTAC 
CAAGATATAG TTACTGCACT GAAATGCAGA AAAGAAGACA AAGAATTGTA TACTGTTGTT 
CAGCATGTGA AGCACTTCAA TGATGTGGTG GAATTTGGTG AGAATCAAGA ATTCACTGAT 
GACATTGAGT ACCTGTTAAG TGGCTTAAAG AGCACTCAGC CTCTAAACAC ACGTTGCCTT 
AGTGTTATTA GCTTGGCTAC TAAATGTGCC ATGCCCAGTT TTCGAATGCA CCTGAGAGCA 
CATGGAATGG TAGCAATGGT CTTTAAAACC TTGGATGATT CCCAGCACCA TCAGAATCTG 
TCCCTTTGTA CAGCTGCCCT CATGTACATA CTGAGTAGAG ATCGTCTGAA CATGGATCTT 
GATAGAGCTA GCCTAGATCT AATGATTCGA CTTCTAGAAC TGGAACAAGA TGCTTCTTCA 
GCTAAGCTAC TGAATGAAAA AGACATGAAC AAAATCAAAG AAAAAATTCG AAGGCTCTGT 
GAAACTGTAC ACAACAAGCA TCTTGATCTA GAAAATATAA CGACTGGGCA TTTAGCTATG 
GAGACCTTGC TGTCCCTTAC TTCTAAACGA GCAGGAGACT GGTTTAAAGA AGAACTCCGG 
CTTTTGGGTG GTCTGGATCA TATTGTAGAT AAAGTGAAAG AATGTGTGGA TCATTTAAGT 
AGAGATGAAG ATGAAGAGAA ACTGGTAGCC TCATTATGGG GAGCAGAGAG ATGTTTACGA 
GTTTTAGAAA GTGTAACAGT GCATAATCCA GAGAATCAAA GCTACTTGAT AGCATATAAA 
GATTCACAAC TCATTGTTTC TTCAGCTAAA GCATTACAGC ATTGTGAAGG ATTGATTCAG 
CAGTATAACC GTGCTGAGAA CAGTATATGC TTACCTGACA GTAAGCCTCT GCCTCACCAG 
AATGTAACCA ACCATGTGGG CAAAGCCGTA GAAGACTGCA TGAGGGCCAT CATTGGGGTA 
TTGCTCAATT TAACTAATGA TAATGAGTGG GGCAGCACCA AAACGGGAGA ACAGGATGGC 
TTGATTGGCA CAGCAATGAA CTGTGTGCTT CAGGTTCCAA AGTACCTACC TCAGGAGCAG 
AGATTTGATA TTCGAGTGCT GGGCTTAGGT CTACTGATAA ATCTAGTGGA GTATAGTGCT 
CGGAATCGGC ACTGTCTTGT CAACATGGAA ACATCATGTT CTTTTGATTC TTCCCTCTGT 
AGTGGAGAAG GAGATGATAG TTTAAGGATA GCTGGACAAA TTCATGCCGT TCAGGCCTTA 
GTGCAGCTAT TCCTTGAGCG AGAACGAGCA GCCCAGTTGG CAGAAAGTAA AACAGATGAA 
TTGATCAAAG ATGCTCCCAC CACTCAGCAT GATAAGAGTG GAGAGTGGCA AGAGACAAGT 
GGAGAAATAC AGTGGGTATC AACTGAAAAG ACTGATGGTA CAGAAGAGAA ACATAAGAAG 
GAAGAGGACG ATGAAGAACT TGACCTCAAT AAAGCCCTTC AGCATGCTGG CAAACACATG 
GAGGATTGCA TTGTGGCCTC ATATACAGCA CTACTTCTCG GGTGTCTCTG CCAGGAGAGT 
CCAATCAATG TGACCACTGT GCGAGAATAT CTGCCAGAAG GCGACTTTTC AATAATGACA 
GAGATGCTCA AAAAATTCCT GAGCTTCATG AATCTTACTT GTGCTGTTGG AACCACAGGC 
CAGAAATCTA TCTCTAGAGT GATTGAATAC TTGGAACATT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( XM_859725.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_864818.3 Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

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CloneID	OCe05141
Clone ID Related Accession (Same CDS sequence)	XM_859725.4 , XM_859725.5
Accession Version	XM_859725.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	wings apart-like protein homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_859725.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT ACTTTTTTTT CCCCCCCTAC AGAGAATATG AAACAGGTGT CAAAATGACA 
TCCAGATTTG GGAAAACATA CAGTAGGAAA GGAGGAAATG GCAGTTCAAA ATTCGATGAA 
GTCTTTTCCA ATAAACGGAC CACCCTTAGC ACAAAATGGG GAGAGACCAC ATTTATGGCT 
AAATTAGGGC AGAAGAGGCC CAATTTCAAA CCAGATATCC AAGAAATTCC GAAGAAACCT 
AAGGTGGAAG AAGAAAATAC TGGAGATCCT TTTGGATTTG ATAGTGATGA TGAGTCTCTA 
CCAGTTTCTT CAAAGAATTT AGCCCAGGGT AAGGGTTCCT CTTACTCAGA ATCTAGTGAA 
GCTGCTCAGC TGGAAGAGGT CACTTCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGCA 
GTGGGCGAAG ACAGTGTTGT ATCTGATAAA TGCTTACTTT TGGAAGAAAC TTTGGTTGGG 
AAAGAAAAGA GCACAAACCG AATTTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA 
TTAGCGACTT CAGATAAAGT GGAGAATTTT AGTGAAGAAT ATGAAAAGAA TAGTCACCAT 
ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACCCAGTG CAGAAACAAC AGTAGCTTCT 
GTATACAAAG CTGATGAAAT CAAGGAAACA AATGACACTT GGAACTCTCA ACTTGGAAAA 
AGGTCAGAAT CTCCATCAGA AACATCTCCC ATTAAGGGAT CTGTAAGAAC TGGTTTGTAT 
GAATGGGATA ATGATTTTGA AGATATCAGA TCAGAAGATT GTATTTTAAG TTTGGATAGT 
GATCCTCTTT TGGAGATGAA GGATGAGGAT TTTAAAAACC GGTTGGAAAA TCTAAATGAA 
GCCATTGAGG AAGACATCAT GCAAAGTGTT CTTAGGCCAA GCAACTGTAG GACGTACTGT 
AGGGCCAATA AAGCAAAATC CTCACAAGGA GCTTCAAATT TTGATAAGCT AATGGACGGA 
ACCAGTCAGG CCTTAGCCAA AGCAAACAGT GAATCAAGTA AGGATGGCCT GAATCAGGCA 
AAGAAAGGCA GTGTAAGTTC TGGGACCAGT TTTAGAGGGA CAGTTGGACG GACTAGAGAT 
TACACTGTTT TACATCCATC TTGCTTGTCA GTTTGTAATG TTACCATACA GGATACTATG 
GAACGGAGCA TGGATGAATT CACTGCATCA ACCCCTGCAG ATTTGGGCGA AGCTGGCCGC 
CTAAGAAAAA AGGCAGATAT TGCGACTTCT AAGACCACTA CTAGATTTCG ACCTAGTAAT 
ACTAAATCTA AAAAGGATGT TAAACTTGAA TTTTTTGGTT TTGAAGATCA TGATGAGACA 
GGAGGTGATG AAGGAGGTTC TGGGAGTTCT AATTACAAAA TTAAGTATTT TGGTTTTGAT 
GATCTCAGTG AAAGTGAAGA TGATGATGAA GATGACTGTC AAGTGGAAAG AAAGACAAGC 
AAAAAAAGAA CTAAAACAGC TCCATCCCCC TCCTTGCAGC CTCCTCCTGA AAGCATTGAT 
AATTCCCAGG ACAGTCAGTC TAGTGCTAAC AATGCAGAGG ACTTGCCTGG TGTGCCTGAA 
AGTGTGAAGA AGCCCCCAAA TAAACAAGGA GATAAATCAA AAGAAAACAC CAGAAAGATT 
TTTAGTGGTC CCAAACGGTC TCCTACAAAA GCTGTATATA ATGCTAGACA TTGGAACCAT 
CCAGACTCTG AAGAGCTGCC TGGGCCACCA ATAGTAAAAC CTCAGAGTGC CACAGTGAGG 
CTGTCTTCAA AGGAACCAAA TCAAAAAGAT GATGGAGTTT TTAAGGCTCC TGCACCACCA 
CCCAAAGTGA TAAAAACTGT GACAATACCT ACTCAGCCCT ACCAAGATAT AGTTACTGCA 
CTGAAATGCA GAAAAGAAGA CAAAGAATTG TATACTGTTG TTCAGCATGT GAAGCACTTC 
AATGATGTGG TGGAATTTGG TGAGAATCAA GAATTCACTG ATGACATTGA GTACCTGTTA 
AGTGGCTTAA AGAGCACTCA GCCTCTAAAC ACACGTTGCC TTAGTGTTAT TAGCTTGGCT 
ACTAAATGTG CCATGCCCAG TTTTCGAATG CACCTGAGAG CACATGGAAT GGTAGCAATG 
GTCTTTAAAA CCTTGGATGA TTCCCAGCAC CATCAGAATC TGTCCCTTTG TACAGCTGCC 
CTCATGTACA TACTGAGTAG AGATCGTCTG AACATGGATC TTGATAGAGC TAGCCTAGAT 
CTAATGATTC GACTTCTAGA ACTGGAACAA GATGCTTCTT CAGCTAAGCT ACTGAATGAA 
AAAGACATGA ACAAAATCAA AGAAAAAATT CGAAGGCTCT GTGAAACTGT ACACAACAAG 
CATCTTGATC TAGAAAATAT AACGACTGGG CATTTAGCTA TGGAGACCTT GCTGTCCCTT 
ACTTCTAAAC GAGCAGGAGA CTGGTTTAAA GAAGAACTCC GGCTTTTGGG TGGTCTGGAT 
CATATTGTAG ATAAAGTGAA AGAATGTGTG GATCATTTAA GTAGAGATGA AGATGAAGAG 
AAACTGGTAG CCTCATTATG GGGAGCAGAG AGATGTTTAC GAGTTTTAGA AAGTGTAACA 
GTGCATAATC CAGAGAATCA AAGCTACTTG ATAGCATATA AAGATTCACA ACTCATTGTT 
TCTTCAGCTA AAGCATTACA GCATTGTGAA GGATTGATTC AGCAGTATAA CCGTGCTGAG 
AACAGTATAT GCTTACCTGA CAGTAAGCCT CTGCCTCACC AGAATGTAAC CAACCATGTG 
GGCAAAGCCG TAGAAGACTG CATGAGGGCC ATCATTGGGG TATTGCTCAA TTTAACTAAT 
GATAATGAGT GGGGCAGCAC CAAAACGGGA GAACAGGATG GCTTGATTGG CACAGCAATG 
AACTGTGTGC TTCAGGTTCC AAAGTACCTA CCTCAGGAGC AGAGATTTGA TATTCGAGTG 
CTGGGCTTAG GTCTACTGAT AAATCTAGTG GAGTATAGTG CTCGGAATCG GCACTGTCTT 
GTCAACATGG AAACATCATG TTCTTTTGAT TCTTCCCTCT GTAGTGGAGA AGGAGATGAT 
AGTTTAAGGA TAGCTGGACA AATTCATGCC GTTCAGGCCT TAGTGCAGCT ATTCCTTGAG 
CGAGAACGAG CAGCCCAGTT GGCAGAAAGT AAAACAGATG AATTGATCAA AGATGCTCCC 
ACCACTCAGC ATGATAAGAG TGGAGAGTGG CAAGAGACAA GTGGAGAAAT ACAGTGGGTA 
TCAACTGAAA AGACTGATGG TACAGAAGAG AAACATAAGA AGGAAGAGGA CGATGAAGAA 
CTTGACCTCA ATAAAGCCCT TCAGCATGCT GGCAAACACA TGGAGGATTG CATTGTGGCC 
TCATATACAG CACTACTTCT CGGGTGTCTC TGCCAGGAGA GTCCAATCAA TGTGACCACT 
GTGCGAGAAT ATCTGCCAGA AGGCGACTTT TCAATAATGA CAGAGATGCT CAAAAAATTC 
CTGAGCTTCA TGAATCTTAC TTGTGCTGTT GGAACCACAG GCCAGAAATC TATCTCTAGA 
GTGATTGAAT ACTTGGAACA TTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_864818.3
CDS	332..3958
Translation	MNSYFFFPPYREYETGVKMTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEENTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVLEANSKISHAVGEDSVVSDKCLLLEETLVGKEKSTNRILEDDASRSSCAKLATSDKVENFSEEYEKNSHHIYKNAEDSTKKPSAETTVASVYKADEIKETNDTWNSQLGKRSESPSETSPIKGSVRTGLYEWDNDFEDIRSEDCILSLDSDPLLEMKDEDFKNRLENLNEAIEEDIMQSVLRPSNCRTYCRANKAKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGSVSSGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDEDDCQVERKTSKKRTKTAPSPSLQPPPESIDNSQDSQSSANNAEDLPGVPESVKKPPNKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPIVKPQSATVRLSSKEPNQKDDGVFKAPAPPPKVIKTVTIPTQPYQDIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEGLIQQYNRAENSICLPDSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTAMNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSLCSGEGDDSLRIAGQIHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEDDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_859725.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_859725.4

ORF Insert Sequence:

ATGAATTCTT ACTTTTTTTT CCCCCCCTAC AGAGAATATG AAACAGGTGT CAAAATGACA 
TCCAGATTTG GGAAAACATA CAGTAGGAAA GGAGGAAATG GCAGTTCAAA ATTCGATGAA 
GTCTTTTCCA ATAAACGGAC CACCCTTAGC ACAAAATGGG GAGAGACCAC ATTTATGGCT 
AAATTAGGGC AGAAGAGGCC CAATTTCAAA CCAGATATCC AAGAAATTCC GAAGAAACCT 
AAGGTGGAAG AAGAAAATAC TGGAGATCCT TTTGGATTTG ATAGTGATGA TGAGTCTCTA 
CCAGTTTCTT CAAAGAATTT AGCCCAGGGT AAGGGTTCCT CTTACTCAGA ATCTAGTGAA 
GCTGCTCAGC TGGAAGAGGT CACTTCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGCA 
GTGGGCGAAG ACAGTGTTGT ATCTGATAAA TGCTTACTTT TGGAAGAAAC TTTGGTTGGG 
AAAGAAAAGA GCACAAACCG AATTTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA 
TTAGCGACTT CAGATAAAGT GGAGAATTTT AGTGAAGAAT ATGAAAAGAA TAGTCACCAT 
ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACCCAGTG CAGAAACAAC AGTAGCTTCT 
GTATACAAAG CTGATGAAAT CAAGGAAACA AATGACACTT GGAACTCTCA ACTTGGAAAA 
AGGTCAGAAT CTCCATCAGA AACATCTCCC ATTAAGGGAT CTGTAAGAAC TGGTTTGTAT 
GAATGGGATA ATGATTTTGA AGATATCAGA TCAGAAGATT GTATTTTAAG TTTGGATAGT 
GATCCTCTTT TGGAGATGAA GGATGAGGAT TTTAAAAACC GGTTGGAAAA TCTAAATGAA 
GCCATTGAGG AAGACATCAT GCAAAGTGTT CTTAGGCCAA GCAACTGTAG GACGTACTGT 
AGGGCCAATA AAGCAAAATC CTCACAAGGA GCTTCAAATT TTGATAAGCT AATGGACGGA 
ACCAGTCAGG CCTTAGCCAA AGCAAACAGT GAATCAAGTA AGGATGGCCT GAATCAGGCA 
AAGAAAGGCA GTGTAAGTTC TGGGACCAGT TTTAGAGGGA CAGTTGGACG GACTAGAGAT 
TACACTGTTT TACATCCATC TTGCTTGTCA GTTTGTAATG TTACCATACA GGATACTATG 
GAACGGAGCA TGGATGAATT CACTGCATCA ACCCCTGCAG ATTTGGGCGA AGCTGGCCGC 
CTAAGAAAAA AGGCAGATAT TGCGACTTCT AAGACCACTA CTAGATTTCG ACCTAGTAAT 
ACTAAATCTA AAAAGGATGT TAAACTTGAA TTTTTTGGTT TTGAAGATCA TGATGAGACA 
GGAGGTGATG AAGGAGGTTC TGGGAGTTCT AATTACAAAA TTAAGTATTT TGGTTTTGAT 
GATCTCAGTG AAAGTGAAGA TGATGATGAA GATGACTGTC AAGTGGAAAG AAAGACAAGC 
AAAAAAAGAA CTAAAACAGC TCCATCCCCC TCCTTGCAGC CTCCTCCTGA AAGCATTGAT 
AATTCCCAGG ACAGTCAGTC TAGTGCTAAC AATGCAGAGG ACTTGCCTGG TGTGCCTGAA 
AGTGTGAAGA AGCCCCCAAA TAAACAAGGA GATAAATCAA AAGAAAACAC CAGAAAGATT 
TTTAGTGGTC CCAAACGGTC TCCTACAAAA GCTGTATATA ATGCTAGACA TTGGAACCAT 
CCAGACTCTG AAGAGCTGCC TGGGCCACCA ATAGTAAAAC CTCAGAGTGC CACAGTGAGG 
CTGTCTTCAA AGGAACCAAA TCAAAAAGAT GATGGAGTTT TTAAGGCTCC TGCACCACCA 
CCCAAAGTGA TAAAAACTGT GACAATACCT ACTCAGCCCT ACCAAGATAT AGTTACTGCA 
CTGAAATGCA GAAAAGAAGA CAAAGAATTG TATACTGTTG TTCAGCATGT GAAGCACTTC 
AATGATGTGG TGGAATTTGG TGAGAATCAA GAATTCACTG ATGACATTGA GTACCTGTTA 
AGTGGCTTAA AGAGCACTCA GCCTCTAAAC ACACGTTGCC TTAGTGTTAT TAGCTTGGCT 
ACTAAATGTG CCATGCCCAG TTTTCGAATG CACCTGAGAG CACATGGAAT GGTAGCAATG 
GTCTTTAAAA CCTTGGATGA TTCCCAGCAC CATCAGAATC TGTCCCTTTG TACAGCTGCC 
CTCATGTACA TACTGAGTAG AGATCGTCTG AACATGGATC TTGATAGAGC TAGCCTAGAT 
CTAATGATTC GACTTCTAGA ACTGGAACAA GATGCTTCTT CAGCTAAGCT ACTGAATGAA 
AAAGACATGA ACAAAATCAA AGAAAAAATT CGAAGGCTCT GTGAAACTGT ACACAACAAG 
CATCTTGATC TAGAAAATAT AACGACTGGG CATTTAGCTA TGGAGACCTT GCTGTCCCTT 
ACTTCTAAAC GAGCAGGAGA CTGGTTTAAA GAAGAACTCC GGCTTTTGGG TGGTCTGGAT 
CATATTGTAG ATAAAGTGAA AGAATGTGTG GATCATTTAA GTAGAGATGA AGATGAAGAG 
AAACTGGTAG CCTCATTATG GGGAGCAGAG AGATGTTTAC GAGTTTTAGA AAGTGTAACA 
GTGCATAATC CAGAGAATCA AAGCTACTTG ATAGCATATA AAGATTCACA ACTCATTGTT 
TCTTCAGCTA AAGCATTACA GCATTGTGAA GGATTGATTC AGCAGTATAA CCGTGCTGAG 
AACAGTATAT GCTTACCTGA CAGTAAGCCT CTGCCTCACC AGAATGTAAC CAACCATGTG 
GGCAAAGCCG TAGAAGACTG CATGAGGGCC ATCATTGGGG TATTGCTCAA TTTAACTAAT 
GATAATGAGT GGGGCAGCAC CAAAACGGGA GAACAGGATG GCTTGATTGG CACAGCAATG 
AACTGTGTGC TTCAGGTTCC AAAGTACCTA CCTCAGGAGC AGAGATTTGA TATTCGAGTG 
CTGGGCTTAG GTCTACTGAT AAATCTAGTG GAGTATAGTG CTCGGAATCG GCACTGTCTT 
GTCAACATGG AAACATCATG TTCTTTTGAT TCTTCCCTCT GTAGTGGAGA AGGAGATGAT 
AGTTTAAGGA TAGCTGGACA AATTCATGCC GTTCAGGCCT TAGTGCAGCT ATTCCTTGAG 
CGAGAACGAG CAGCCCAGTT GGCAGAAAGT AAAACAGATG AATTGATCAA AGATGCTCCC 
ACCACTCAGC ATGATAAGAG TGGAGAGTGG CAAGAGACAA GTGGAGAAAT ACAGTGGGTA 
TCAACTGAAA AGACTGATGG TACAGAAGAG AAACATAAGA AGGAAGAGGA CGATGAAGAA 
CTTGACCTCA ATAAAGCCCT TCAGCATGCT GGCAAACACA TGGAGGATTG CATTGTGGCC 
TCATATACAG CACTACTTCT CGGGTGTCTC TGCCAGGAGA GTCCAATCAA TGTGACCACT 
GTGCGAGAAT ATCTGCCAGA AGGCGACTTT TCAATAATGA CAGAGATGCT CAAAAAATTC 
CTGAGCTTCA TGAATCTTAC TTGTGCTGTT GGAACCACAG GCCAGAAATC TATCTCTAGA 
GTGATTGAAT ACTTGGAACA TTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( XM_859725.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_864818.3 Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

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CloneID	OCe05141
Clone ID Related Accession (Same CDS sequence)	XM_859725.4 , XM_859725.5
Accession Version	XM_859725.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	wings apart-like protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_859725.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT ACTTTTTTTT CCCCCCCTAC AGAGAATATG AAACAGGTGT CAAAATGACA 
TCCAGATTTG GGAAAACATA CAGTAGGAAA GGAGGAAATG GCAGTTCAAA ATTCGATGAA 
GTCTTTTCCA ATAAACGGAC CACCCTTAGC ACAAAATGGG GAGAGACCAC ATTTATGGCT 
AAATTAGGGC AGAAGAGGCC CAATTTCAAA CCAGATATCC AAGAAATTCC GAAGAAACCT 
AAGGTGGAAG AAGAAAATAC TGGAGATCCT TTTGGATTTG ATAGTGATGA TGAGTCTCTA 
CCAGTTTCTT CAAAGAATTT AGCCCAGGGT AAGGGTTCCT CTTACTCAGA ATCTAGTGAA 
GCTGCTCAGC TGGAAGAGGT CACTTCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGCA 
GTGGGCGAAG ACAGTGTTGT ATCTGATAAA TGCTTACTTT TGGAAGAAAC TTTGGTTGGG 
AAAGAAAAGA GCACAAACCG AATTTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA 
TTAGCGACTT CAGATAAAGT GGAGAATTTT AGTGAAGAAT ATGAAAAGAA TAGTCACCAT 
ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACCCAGTG CAGAAACAAC AGTAGCTTCT 
GTATACAAAG CTGATGAAAT CAAGGAAACA AATGACACTT GGAACTCTCA ACTTGGAAAA 
AGGTCAGAAT CTCCATCAGA AACATCTCCC ATTAAGGGAT CTGTAAGAAC TGGTTTGTAT 
GAATGGGATA ATGATTTTGA AGATATCAGA TCAGAAGATT GTATTTTAAG TTTGGATAGT 
GATCCTCTTT TGGAGATGAA GGATGAGGAT TTTAAAAACC GGTTGGAAAA TCTAAATGAA 
GCCATTGAGG AAGACATCAT GCAAAGTGTT CTTAGGCCAA GCAACTGTAG GACGTACTGT 
AGGGCCAATA AAGCAAAATC CTCACAAGGA GCTTCAAATT TTGATAAGCT AATGGACGGA 
ACCAGTCAGG CCTTAGCCAA AGCAAACAGT GAATCAAGTA AGGATGGCCT GAATCAGGCA 
AAGAAAGGCA GTGTAAGTTC TGGGACCAGT TTTAGAGGGA CAGTTGGACG GACTAGAGAT 
TACACTGTTT TACATCCATC TTGCTTGTCA GTTTGTAATG TTACCATACA GGATACTATG 
GAACGGAGCA TGGATGAATT CACTGCATCA ACCCCTGCAG ATTTGGGCGA AGCTGGCCGC 
CTAAGAAAAA AGGCAGATAT TGCGACTTCT AAGACCACTA CTAGATTTCG ACCTAGTAAT 
ACTAAATCTA AAAAGGATGT TAAACTTGAA TTTTTTGGTT TTGAAGATCA TGATGAGACA 
GGAGGTGATG AAGGAGGTTC TGGGAGTTCT AATTACAAAA TTAAGTATTT TGGTTTTGAT 
GATCTCAGTG AAAGTGAAGA TGATGATGAA GATGACTGTC AAGTGGAAAG AAAGACAAGC 
AAAAAAAGAA CTAAAACAGC TCCATCCCCC TCCTTGCAGC CTCCTCCTGA AAGCATTGAT 
AATTCCCAGG ACAGTCAGTC TAGTGCTAAC AATGCAGAGG ACTTGCCTGG TGTGCCTGAA 
AGTGTGAAGA AGCCCCCAAA TAAACAAGGA GATAAATCAA AAGAAAACAC CAGAAAGATT 
TTTAGTGGTC CCAAACGGTC TCCTACAAAA GCTGTATATA ATGCTAGACA TTGGAACCAT 
CCAGACTCTG AAGAGCTGCC TGGGCCACCA ATAGTAAAAC CTCAGAGTGC CACAGTGAGG 
CTGTCTTCAA AGGAACCAAA TCAAAAAGAT GATGGAGTTT TTAAGGCTCC TGCACCACCA 
CCCAAAGTGA TAAAAACTGT GACAATACCT ACTCAGCCCT ACCAAGATAT AGTTACTGCA 
CTGAAATGCA GAAAAGAAGA CAAAGAATTG TATACTGTTG TTCAGCATGT GAAGCACTTC 
AATGATGTGG TGGAATTTGG TGAGAATCAA GAATTCACTG ATGACATTGA GTACCTGTTA 
AGTGGCTTAA AGAGCACTCA GCCTCTAAAC ACACGTTGCC TTAGTGTTAT TAGCTTGGCT 
ACTAAATGTG CCATGCCCAG TTTTCGAATG CACCTGAGAG CACATGGAAT GGTAGCAATG 
GTCTTTAAAA CCTTGGATGA TTCCCAGCAC CATCAGAATC TGTCCCTTTG TACAGCTGCC 
CTCATGTACA TACTGAGTAG AGATCGTCTG AACATGGATC TTGATAGAGC TAGCCTAGAT 
CTAATGATTC GACTTCTAGA ACTGGAACAA GATGCTTCTT CAGCTAAGCT ACTGAATGAA 
AAAGACATGA ACAAAATCAA AGAAAAAATT CGAAGGCTCT GTGAAACTGT ACACAACAAG 
CATCTTGATC TAGAAAATAT AACGACTGGG CATTTAGCTA TGGAGACCTT GCTGTCCCTT 
ACTTCTAAAC GAGCAGGAGA CTGGTTTAAA GAAGAACTCC GGCTTTTGGG TGGTCTGGAT 
CATATTGTAG ATAAAGTGAA AGAATGTGTG GATCATTTAA GTAGAGATGA AGATGAAGAG 
AAACTGGTAG CCTCATTATG GGGAGCAGAG AGATGTTTAC GAGTTTTAGA AAGTGTAACA 
GTGCATAATC CAGAGAATCA AAGCTACTTG ATAGCATATA AAGATTCACA ACTCATTGTT 
TCTTCAGCTA AAGCATTACA GCATTGTGAA GGATTGATTC AGCAGTATAA CCGTGCTGAG 
AACAGTATAT GCTTACCTGA CAGTAAGCCT CTGCCTCACC AGAATGTAAC CAACCATGTG 
GGCAAAGCCG TAGAAGACTG CATGAGGGCC ATCATTGGGG TATTGCTCAA TTTAACTAAT 
GATAATGAGT GGGGCAGCAC CAAAACGGGA GAACAGGATG GCTTGATTGG CACAGCAATG 
AACTGTGTGC TTCAGGTTCC AAAGTACCTA CCTCAGGAGC AGAGATTTGA TATTCGAGTG 
CTGGGCTTAG GTCTACTGAT AAATCTAGTG GAGTATAGTG CTCGGAATCG GCACTGTCTT 
GTCAACATGG AAACATCATG TTCTTTTGAT TCTTCCCTCT GTAGTGGAGA AGGAGATGAT 
AGTTTAAGGA TAGCTGGACA AATTCATGCC GTTCAGGCCT TAGTGCAGCT ATTCCTTGAG 
CGAGAACGAG CAGCCCAGTT GGCAGAAAGT AAAACAGATG AATTGATCAA AGATGCTCCC 
ACCACTCAGC ATGATAAGAG TGGAGAGTGG CAAGAGACAA GTGGAGAAAT ACAGTGGGTA 
TCAACTGAAA AGACTGATGG TACAGAAGAG AAACATAAGA AGGAAGAGGA CGATGAAGAA 
CTTGACCTCA ATAAAGCCCT TCAGCATGCT GGCAAACACA TGGAGGATTG CATTGTGGCC 
TCATATACAG CACTACTTCT CGGGTGTCTC TGCCAGGAGA GTCCAATCAA TGTGACCACT 
GTGCGAGAAT ATCTGCCAGA AGGCGACTTT TCAATAATGA CAGAGATGCT CAAAAAATTC 
CTGAGCTTCA TGAATCTTAC TTGTGCTGTT GGAACCACAG GCCAGAAATC TATCTCTAGA 
GTGATTGAAT ACTTGGAACA TTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_864818.3
CDS	1163..4789
Translation	MNSYFFFPPYREYETGVKMTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEENTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVLEANSKISHAVGEDSVVSDKCLLLEETLVGKEKSTNRILEDDASRSSCAKLATSDKVENFSEEYEKNSHHIYKNAEDSTKKPSAETTVASVYKADEIKETNDTWNSQLGKRSESPSETSPIKGSVRTGLYEWDNDFEDIRSEDCILSLDSDPLLEMKDEDFKNRLENLNEAIEEDIMQSVLRPSNCRTYCRANKAKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGSVSSGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDEDDCQVERKTSKKRTKTAPSPSLQPPPESIDNSQDSQSSANNAEDLPGVPESVKKPPNKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPIVKPQSATVRLSSKEPNQKDDGVFKAPAPPPKVIKTVTIPTQPYQDIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEGLIQQYNRAENSICLPDSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTAMNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSLCSGEGDDSLRIAGQIHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEDDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_859725.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_859725.5

ORF Insert Sequence:

ATGAATTCTT ACTTTTTTTT CCCCCCCTAC AGAGAATATG AAACAGGTGT CAAAATGACA 
TCCAGATTTG GGAAAACATA CAGTAGGAAA GGAGGAAATG GCAGTTCAAA ATTCGATGAA 
GTCTTTTCCA ATAAACGGAC CACCCTTAGC ACAAAATGGG GAGAGACCAC ATTTATGGCT 
AAATTAGGGC AGAAGAGGCC CAATTTCAAA CCAGATATCC AAGAAATTCC GAAGAAACCT 
AAGGTGGAAG AAGAAAATAC TGGAGATCCT TTTGGATTTG ATAGTGATGA TGAGTCTCTA 
CCAGTTTCTT CAAAGAATTT AGCCCAGGGT AAGGGTTCCT CTTACTCAGA ATCTAGTGAA 
GCTGCTCAGC TGGAAGAGGT CACTTCTGTA CTTGAAGCTA ATAGCAAAAT TAGTCATGCA 
GTGGGCGAAG ACAGTGTTGT ATCTGATAAA TGCTTACTTT TGGAAGAAAC TTTGGTTGGG 
AAAGAAAAGA GCACAAACCG AATTTTAGAA GATGATGCAA GCAGAAGTAG CTGTGCTAAA 
TTAGCGACTT CAGATAAAGT GGAGAATTTT AGTGAAGAAT ATGAAAAGAA TAGTCACCAT 
ATTTACAAAA ATGCTGAAGA CAGTACTAAG AAACCCAGTG CAGAAACAAC AGTAGCTTCT 
GTATACAAAG CTGATGAAAT CAAGGAAACA AATGACACTT GGAACTCTCA ACTTGGAAAA 
AGGTCAGAAT CTCCATCAGA AACATCTCCC ATTAAGGGAT CTGTAAGAAC TGGTTTGTAT 
GAATGGGATA ATGATTTTGA AGATATCAGA TCAGAAGATT GTATTTTAAG TTTGGATAGT 
GATCCTCTTT TGGAGATGAA GGATGAGGAT TTTAAAAACC GGTTGGAAAA TCTAAATGAA 
GCCATTGAGG AAGACATCAT GCAAAGTGTT CTTAGGCCAA GCAACTGTAG GACGTACTGT 
AGGGCCAATA AAGCAAAATC CTCACAAGGA GCTTCAAATT TTGATAAGCT AATGGACGGA 
ACCAGTCAGG CCTTAGCCAA AGCAAACAGT GAATCAAGTA AGGATGGCCT GAATCAGGCA 
AAGAAAGGCA GTGTAAGTTC TGGGACCAGT TTTAGAGGGA CAGTTGGACG GACTAGAGAT 
TACACTGTTT TACATCCATC TTGCTTGTCA GTTTGTAATG TTACCATACA GGATACTATG 
GAACGGAGCA TGGATGAATT CACTGCATCA ACCCCTGCAG ATTTGGGCGA AGCTGGCCGC 
CTAAGAAAAA AGGCAGATAT TGCGACTTCT AAGACCACTA CTAGATTTCG ACCTAGTAAT 
ACTAAATCTA AAAAGGATGT TAAACTTGAA TTTTTTGGTT TTGAAGATCA TGATGAGACA 
GGAGGTGATG AAGGAGGTTC TGGGAGTTCT AATTACAAAA TTAAGTATTT TGGTTTTGAT 
GATCTCAGTG AAAGTGAAGA TGATGATGAA GATGACTGTC AAGTGGAAAG AAAGACAAGC 
AAAAAAAGAA CTAAAACAGC TCCATCCCCC TCCTTGCAGC CTCCTCCTGA AAGCATTGAT 
AATTCCCAGG ACAGTCAGTC TAGTGCTAAC AATGCAGAGG ACTTGCCTGG TGTGCCTGAA 
AGTGTGAAGA AGCCCCCAAA TAAACAAGGA GATAAATCAA AAGAAAACAC CAGAAAGATT 
TTTAGTGGTC CCAAACGGTC TCCTACAAAA GCTGTATATA ATGCTAGACA TTGGAACCAT 
CCAGACTCTG AAGAGCTGCC TGGGCCACCA ATAGTAAAAC CTCAGAGTGC CACAGTGAGG 
CTGTCTTCAA AGGAACCAAA TCAAAAAGAT GATGGAGTTT TTAAGGCTCC TGCACCACCA 
CCCAAAGTGA TAAAAACTGT GACAATACCT ACTCAGCCCT ACCAAGATAT AGTTACTGCA 
CTGAAATGCA GAAAAGAAGA CAAAGAATTG TATACTGTTG TTCAGCATGT GAAGCACTTC 
AATGATGTGG TGGAATTTGG TGAGAATCAA GAATTCACTG ATGACATTGA GTACCTGTTA 
AGTGGCTTAA AGAGCACTCA GCCTCTAAAC ACACGTTGCC TTAGTGTTAT TAGCTTGGCT 
ACTAAATGTG CCATGCCCAG TTTTCGAATG CACCTGAGAG CACATGGAAT GGTAGCAATG 
GTCTTTAAAA CCTTGGATGA TTCCCAGCAC CATCAGAATC TGTCCCTTTG TACAGCTGCC 
CTCATGTACA TACTGAGTAG AGATCGTCTG AACATGGATC TTGATAGAGC TAGCCTAGAT 
CTAATGATTC GACTTCTAGA ACTGGAACAA GATGCTTCTT CAGCTAAGCT ACTGAATGAA 
AAAGACATGA ACAAAATCAA AGAAAAAATT CGAAGGCTCT GTGAAACTGT ACACAACAAG 
CATCTTGATC TAGAAAATAT AACGACTGGG CATTTAGCTA TGGAGACCTT GCTGTCCCTT 
ACTTCTAAAC GAGCAGGAGA CTGGTTTAAA GAAGAACTCC GGCTTTTGGG TGGTCTGGAT 
CATATTGTAG ATAAAGTGAA AGAATGTGTG GATCATTTAA GTAGAGATGA AGATGAAGAG 
AAACTGGTAG CCTCATTATG GGGAGCAGAG AGATGTTTAC GAGTTTTAGA AAGTGTAACA 
GTGCATAATC CAGAGAATCA AAGCTACTTG ATAGCATATA AAGATTCACA ACTCATTGTT 
TCTTCAGCTA AAGCATTACA GCATTGTGAA GGATTGATTC AGCAGTATAA CCGTGCTGAG 
AACAGTATAT GCTTACCTGA CAGTAAGCCT CTGCCTCACC AGAATGTAAC CAACCATGTG 
GGCAAAGCCG TAGAAGACTG CATGAGGGCC ATCATTGGGG TATTGCTCAA TTTAACTAAT 
GATAATGAGT GGGGCAGCAC CAAAACGGGA GAACAGGATG GCTTGATTGG CACAGCAATG 
AACTGTGTGC TTCAGGTTCC AAAGTACCTA CCTCAGGAGC AGAGATTTGA TATTCGAGTG 
CTGGGCTTAG GTCTACTGAT AAATCTAGTG GAGTATAGTG CTCGGAATCG GCACTGTCTT 
GTCAACATGG AAACATCATG TTCTTTTGAT TCTTCCCTCT GTAGTGGAGA AGGAGATGAT 
AGTTTAAGGA TAGCTGGACA AATTCATGCC GTTCAGGCCT TAGTGCAGCT ATTCCTTGAG 
CGAGAACGAG CAGCCCAGTT GGCAGAAAGT AAAACAGATG AATTGATCAA AGATGCTCCC 
ACCACTCAGC ATGATAAGAG TGGAGAGTGG CAAGAGACAA GTGGAGAAAT ACAGTGGGTA 
TCAACTGAAA AGACTGATGG TACAGAAGAG AAACATAAGA AGGAAGAGGA CGATGAAGAA 
CTTGACCTCA ATAAAGCCCT TCAGCATGCT GGCAAACACA TGGAGGATTG CATTGTGGCC 
TCATATACAG CACTACTTCT CGGGTGTCTC TGCCAGGAGA GTCCAATCAA TGTGACCACT 
GTGCGAGAAT ATCTGCCAGA AGGCGACTTT TCAATAATGA CAGAGATGCT CAAAAAATTC 
CTGAGCTTCA TGAATCTTAC TTGTGCTGTT GGAACCACAG GCCAGAAATC TATCTCTAGA 
GTGATTGAAT ACTTGGAACA TTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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WAPL cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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