ckap5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ckap5
Entrez Gene ID	492614
Full Name	cytoskeleton associated protein 5
Synonyms	im:7143385,wu:fb14c01,wu:fb59e03,wu:fi33f02,zgc:123012
General protein information	Preferred Names cytoskeleton associated protein 5 Names cytoskeleton-associated protein 5 microtubule-associated protein 215
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005174247.3	XP_005174304.1	cytoskeleton-associated protein 5 isoform X2
XM_005174247.4	XP_005174304.1	cytoskeleton-associated protein 5 isoform X2
XM_009297902.2	XP_009296177.1	cytoskeleton-associated protein 5 isoform X3
XM_009297902.3	XP_009296177.1	cytoskeleton-associated protein 5 isoform X3
NM_001037667.3	NP_001032756.3	cytoskeleton-associated protein 5
XM_009297901.2	XP_009296176.1	cytoskeleton-associated protein 5 isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011162	XP_309487.4
Canis lupus familiaris (dog)	CKAP5	XP_005631330.1
Rattus norvegicus (Norway rat)	Ckap5	XP_006234666.1
Xenopus tropicalis (tropical clawed frog)	ckap5	NP_001120559.1
Oryza sativa (rice)	Os01g0816400	NP_001044618.1
Homo sapiens (human)	CKAP5	NP_001008938.1
Pan troglodytes (chimpanzee)	CKAP5	XP_001165890.1
Arabidopsis thaliana (thale cress)	MOR1	NP_565811.2
Macaca mulatta (Rhesus monkey)	CKAP5	XP_002808118.1
Bos taurus (cattle)	CKAP5	NP_001178292.1
Mus musculus (house mouse)	Ckap5	NP_001159461.1
Gallus gallus (chicken)	CKAP5	NP_001186575.1
Danio rerio (zebrafish)	ckap5	NP_001032756.2
Drosophila melanogaster (fruit fly)	msps	NP_001163627.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of zebrafish magnetoreceptor and cryptochrome homologs.
Zhou Z, Peng X, Chen J, Wu X, Wang Y, Hong Y
Science China. Life sciences59(12)1324-1331(2016 Dec)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Molecular evolution and functional divergence of zebrafish (Danio rerio) cryptochrome genes.
Liu C, Hu J, Qu C, Wang L, Huang G, Niu P, Zhong Z, Hong F, Wang G, Postlethwait JH, Wang H
Scientific reports58113(2015 Jan)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ckap5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ckap5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa41392	NM_001037667.3 Latest version!	Danio rerio cytoskeleton associated protein 5 (ckap5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa29089	XM_005174247.4 Latest version!	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa41390	XM_009297901.2 Latest version!	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa41391	XM_009297902.3 Latest version!	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODa29089	XM_005174247.3	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa41391	XM_009297902.2	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ckap5 ( NM_001037667.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032756.3 Danio rerio cytoskeleton associated protein 5 (ckap5), mRNA.

Price & Availability↑

CloneID	ODa41392
Clone ID Related Accession (Same CDS sequence)	NM_001037667.3
Accession Version	NM_001037667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1501862400000
Organism	Danio rerio(zebrafish)
Product	cytoskeleton-associated protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP236801.8. On Nov 22, 2014 this sequence version replaced NM_001037667.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because there was insufficient transcript data from the Tuebingen strain. The extent of the RefSeq protein is supported by homologous protein data. ##Evidence-Data-START## CDS exon combination :: GDQH01004548.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGG TTGTTGCAGG AAAGAAACCT CCTGTTAAGG CTGGCGCAAA AGATGAGGAG 
GATAAGTCTG GTCCTATTTT CATCCTCGTT CCCAATGGCA AAGAGCAGAG AATCAAAGAA 
GAGAAGTCAT TAAAGATTCT GAAGTGGAAC TTCATTACTC CTCGTGACGA GTACGTAGAG 
CAGCTCAAAA CTCAAATGTC AACATGTCTG GCCAAGTGGC TACAAGACGA GCTCTTCCAC 
TTCGACTTTC AGCATCACGT AAAGGCTATC GGAGCCATGA TTGAGCACAT GGAGGCCGAG 
TCTGAGGCTG TGATTGGCTG TCTGGACCTG GTGTTGAAGT GGTTTACTCT GCGCTTCTTT 
GACACCAACA CTAGTGTGAT CATGAAAACC CTGGAGTTCC TCAAGATGCT CTTCACCATG 
CTGAGCAGGA AAAACTACCA GCTCAATGAC TACGAGGCCT CGTCTTTCAT TCCTTACCTG 
ATCCTTAAGG TTGGAGAGTC TAAAGATGTT GTGCGCAAAG ATGTCCGTGC TATTTTGGCG 
ATGCTGTGTA AAGTCTACGC AGCGAGCAAA GTATTCCCTT ACCTTATGGA TGGGACCAAA 
TCAAAGAACT CAAAGCAAAG ATCTGAATGT CTCGAGGAGC TTGGCTGCCT GATTGAGAAC 
TTCGGTATGA ATGTATGCCA ACCGACTCCG GCCAAGGCTC TGAAAGAAAT CGCTGTCCAT 
ATTGGAGACC GTGACACTAC AGTCCGCAAT GCTGCCCTTA ATACTGTCGT AGCGGCCTAT 
AATGCCTGTG GAGACCAAGT CTTCAAACTC ATCGGCAATC TCTCCGAGAA AGAAATGAGC 
ATGCTTGAAG AGAGAATCAA ACGTTCGGCC AAGAAGACTC CAGCACCCTC AACCAAACAG 
GAGCGGCCCC AGAGGGAGCA TCCCACAAAT CCCAATGCAA CCTTTCTACG CAAGCCTGCT 
CAAGAGGAAG TGCCCAACAA GCTTAATCAA GCTCGGCAGC AGAACGCTCA CTTGGAGCAG 
TCGGCTCCAT CCATCCCGAA AGAGTTTCAG CTGGATCTGG ATGTCTTCGA GAACAACCAC 
ACGTGTGCCA GCGACATCCC TGACCTGGTG CAGCACAAAC TGGATGAAGT TCTGGAGCCG 
GTCATGATTC CTGAGCGCAA AATTCATTCG GTCTCTCCTC ATTTTGATGA CATTCACAAT 
AGCACTGCCT CCACTATCAA CTTTGTCATC TCCCAGGTGG CTAGTGGAGA CATTAACACC 
AGTATCCAGG CTTTGGCACA GATTGATGAG GTTTTGAGGC AGGCAGACAA AGCAGAAGCC 
ATGTCTGGAC ACATCGACCA GTTCCTCATC GCCACCTTCA TGCAGCTGCG GCTTATTTAC 
AACACACACA TGGCTGACGA TCGGCTGGAC AAGAAGGAAA TCTTCAAACT CTACAGTTGC 
ATCATCGGAA ACATGCTATC TCTGTTCTCG ATGGAGAGTC TGGCACGGGA GGCCTCGATG 
GGCGTCCTGA AGGACCTGAT GCACGGTTTG ATCACACTAA TGTTGGACTC CAGAGTGGAG 
GACATAGAGG ACGGACAGCA GCTCATTCGC TCTGTCAACC TGCTCATGGT TCGAGTGCTA 
GAGAAATCTG ATCAGACCAA CATTTTGAGT GCTTTGCTAG TGCTGCTGCA GGACAGTCTT 
ATCAGCACCG CCGGCTCACC CATGTTCTCT GAACTTGTCA TGAAGTGTCT ATGGAGAATG 
ATCCGTTTCC TACCCCAGAC CATCAACAGT ATTAATCTGG ATCGTATCCT GCTGGACGTG 
CACAATTTCA TGAAGGTTTT CCCTAAAGAG AAGCTGAAGC AACTGAAGAG TGACGTTCCT 
CATCGGACAC TGAAGACACT GCTGCACACG CTCTGCAGAC TCACAGGCGC CAAGATATTA 
GACCACCTGT CCATGATCGA GAACCGCAAT GAGTCAGAGT TGGAAGCGCA TCTGAGGCGA 
GTGGTCAAAC ATTCGGCAAA CCTCTCAGGC CTGAAGTCGG ACAAGGGCAC GGAGAAGGGA 
GCACTCCGAT CGGATGACAA GATGATCAAG GCGAAAGTGA GTGACATCCT GTCTGAGATC 
TTCAAAAAGA TCGGCTCCAA GGAAAACACA AAAGAGGGTT TGACGGAGTT GTACGAGTAC 
AAGCAGAAGT ATTCAGATGC AGACCTGGAG CCCTTCCTAA GGAACACATC TCAGTTCTTT 
CAGAGCTATG TGGAGCGAGG CCTGCGCATG ATCGAGTCTG AGCGTGAGGG AAAGGGCCGC 
ATCCAGACCT CTACAGTGAT CGCGCAGCAC AGCACGGAGT CATTTGTGCC GAGCTCCAGC 
TCTGTGCCCG TCAGCAGTAA TGGAGAAGAT CTAAACGCTG CCGCTTACTA CGAGAGACTG 
AAGATCTTAC GACAGCGGCG CGGGCTGGAG AACTCAGCGC CTGAAGATGA GCGTCCACCC 
CTCAGCTCCC TGAGACCGTC AGTCGCCTCC TCCACAGACA TGCTTCACAG CAAACTATCC 
CAGCTGAAGG AGTCCCGCGA GCACTTCCAG CAGGAGCAGT CGCATTCGCA TAGCCCCACG 
CGCTCCTCCT CACCCGCCTC CAACCTGGAC GACCTCAAGA AGAGGCTGGA GAGGATCAAG 
AGCAACCGCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032756.3
CDS	123..6137
Translation	MGDDSEWMKLPIDQKCEHKVWKARLSGYEEALKLFQKLDEKSPEWSKYLGLIKKFVTESNAVAQLKGLEAALAFIENAHVAGKTVGEVVSGVVNKVFNQPKARAKELGTEICLMYIEIEKAEVVQDELIKGLDNKNPKIVVACIEALRKALCEFGSKIITLKPVVKVLPKLFESREKAVRDEAKLLAVEIYRWIRDALRAPLQNINSVQLKELEEEWVKVPATAPKQIRFLRSQQDLKAKFEQQQAAGGDEADGDDEEVAEAVQVDAYELLEAVEILSKLPKDFYEKIEAKKWQERKEALEAVEALTKNPKLESGDYGDLVRALKKVIGKDANVMLVSMAAKCLAGLATGLRKKFGTYAGLVVPTILEKFKEKKPQVVQALQEAIDAVFLTTTLQNISEDVLAVMDNKNPSIKQQASLFLARSFRHCTPSTLPKSVLKPFCAAFLKQVNDSAPEVRDAAFEALGTAMKVIGEKAVNPFLTDVDKLKLDKIKECADKVELIGKKGGGGGEKKERPAAKASPPVEAPAKTSGPPKKSAPAKAAGPPKKGKPAAAPSAKSKKAPETKEIIETELSPEVCEEKAAAVLPASCMQLLDSANWKERLASMEEFQKAVEQMDKSEMPCQALVKMLAKKPGWKETNFQVMQMKLSIVGLVAQKGQFSKTSALVVLDGLVDKIGDVKCGGNAKEALTAIGEACSLPWTAEQVVSIAFAQKNPKNQAETLNWLANAMKEFGFAGINVKGFINNVKTALGATNPAVRTSAIALLGVMYLYMGAPLRMFFEDEKPALLSQIDAEFVKMQGQSPPAPTRGAKKAGAEEDGDVADEDEADGGAGDIMDMLPRTDISDKITSEMVSKISDKNWKIRKEGLDEVAAVISEAKFIQANIGELPMALKGRLNDSNKLLVQQTLNILQQIAIAMGPSLKQHVKNLGIPVVTVLGDSKPNVRATAMATLNAWVEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLSEKLPTLRTVPADLMLCVPHLYTCLEDRSGDVRKKAQDALPTFMMHLGFDKMTKATGKLKPASKDQVVGMLDKARAVMPAKPAAPAKAAASKPASSAPAAKTASAPARNHSPVDDYSEPEPKPDTKKAKPAGPAAKKVVAGKKPPVKAGAKDEEDKSGPIFILVPNGKEQRIKEEKSLKILKWNFITPRDEYVEQLKTQMSTCLAKWLQDELFHFDFQHHVKAIGAMIEHMEAESEAVIGCLDLVLKWFTLRFFDTNTSVIMKTLEFLKMLFTMLSRKNYQLNDYEASSFIPYLILKVGESKDVVRKDVRAILAMLCKVYAASKVFPYLMDGTKSKNSKQRSECLEELGCLIENFGMNVCQPTPAKALKEIAVHIGDRDTTVRNAALNTVVAAYNACGDQVFKLIGNLSEKEMSMLEERIKRSAKKTPAPSTKQERPQREHPTNPNATFLRKPAQEEVPNKLNQARQQNAHLEQSAPSIPKEFQLDLDVFENNHTCASDIPDLVQHKLDEVLEPVMIPERKIHSVSPHFDDIHNSTASTINFVISQVASGDINTSIQALAQIDEVLRQADKAEAMSGHIDQFLIATFMQLRLIYNTHMADDRLDKKEIFKLYSCIIGNMLSLFSMESLAREASMGVLKDLMHGLITLMLDSRVEDIEDGQQLIRSVNLLMVRVLEKSDQTNILSALLVLLQDSLISTAGSPMFSELVMKCLWRMIRFLPQTINSINLDRILLDVHNFMKVFPKEKLKQLKSDVPHRTLKTLLHTLCRLTGAKILDHLSMIENRNESELEAHLRRVVKHSANLSGLKSDKGTEKGALRSDDKMIKAKVSDILSEIFKKIGSKENTKEGLTELYEYKQKYSDADLEPFLRNTSQFFQSYVERGLRMIESEREGKGRIQTSTVIAQHSTESFVPSSSSVPVSSNGEDLNAAAYYERLKILRQRRGLENSAPEDERPPLSSLRPSVASSTDMLHSKLSQLKESREHFQQEQSHSHSPTRSSSPASNLDDLKKRLERIKSNRQ

Target ORF information:

RefSeq Version	NM_001037667.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytoskeleton associated protein 5 (ckap5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037667.3

ORF Insert Sequence:

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGG TTGTTGCAGG AAAGAAACCT CCTGTTAAGG CTGGCGCAAA AGATGAGGAG 
GATAAGTCTG GTCCTATTTT CATCCTCGTT CCCAATGGCA AAGAGCAGAG AATCAAAGAA 
GAGAAGTCAT TAAAGATTCT GAAGTGGAAC TTCATTACTC CTCGTGACGA GTACGTAGAG 
CAGCTCAAAA CTCAAATGTC AACATGTCTG GCCAAGTGGC TACAAGACGA GCTCTTCCAC 
TTCGACTTTC AGCATCACGT AAAGGCTATC GGAGCCATGA TTGAGCACAT GGAGGCCGAG 
TCTGAGGCTG TGATTGGCTG TCTGGACCTG GTGTTGAAGT GGTTTACTCT GCGCTTCTTT 
GACACCAACA CTAGTGTGAT CATGAAAACC CTGGAGTTCC TCAAGATGCT CTTCACCATG 
CTGAGCAGGA AAAACTACCA GCTCAATGAC TACGAGGCCT CGTCTTTCAT TCCTTACCTG 
ATCCTTAAGG TTGGAGAGTC TAAAGATGTT GTGCGCAAAG ATGTCCGTGC TATTTTGGCG 
ATGCTGTGTA AAGTCTACGC AGCGAGCAAA GTATTCCCTT ACCTTATGGA TGGGACCAAA 
TCAAAGAACT CAAAGCAAAG ATCTGAATGT CTCGAGGAGC TTGGCTGCCT GATTGAGAAC 
TTCGGTATGA ATGTATGCCA ACCGACTCCG GCCAAGGCTC TGAAAGAAAT CGCTGTCCAT 
ATTGGAGACC GTGACACTAC AGTCCGCAAT GCTGCCCTTA ATACTGTCGT AGCGGCCTAT 
AATGCCTGTG GAGACCAAGT CTTCAAACTC ATCGGCAATC TCTCCGAGAA AGAAATGAGC 
ATGCTTGAAG AGAGAATCAA ACGTTCGGCC AAGAAGACTC CAGCACCCTC AACCAAACAG 
GAGCGGCCCC AGAGGGAGCA TCCCACAAAT CCCAATGCAA CCTTTCTACG CAAGCCTGCT 
CAAGAGGAAG TGCCCAACAA GCTTAATCAA GCTCGGCAGC AGAACGCTCA CTTGGAGCAG 
TCGGCTCCAT CCATCCCGAA AGAGTTTCAG CTGGATCTGG ATGTCTTCGA GAACAACCAC 
ACGTGTGCCA GCGACATCCC TGACCTGGTG CAGCACAAAC TGGATGAAGT TCTGGAGCCG 
GTCATGATTC CTGAGCGCAA AATTCATTCG GTCTCTCCTC ATTTTGATGA CATTCACAAT 
AGCACTGCCT CCACTATCAA CTTTGTCATC TCCCAGGTGG CTAGTGGAGA CATTAACACC 
AGTATCCAGG CTTTGGCACA GATTGATGAG GTTTTGAGGC AGGCAGACAA AGCAGAAGCC 
ATGTCTGGAC ACATCGACCA GTTCCTCATC GCCACCTTCA TGCAGCTGCG GCTTATTTAC 
AACACACACA TGGCTGACGA TCGGCTGGAC AAGAAGGAAA TCTTCAAACT CTACAGTTGC 
ATCATCGGAA ACATGCTATC TCTGTTCTCG ATGGAGAGTC TGGCACGGGA GGCCTCGATG 
GGCGTCCTGA AGGACCTGAT GCACGGTTTG ATCACACTAA TGTTGGACTC CAGAGTGGAG 
GACATAGAGG ACGGACAGCA GCTCATTCGC TCTGTCAACC TGCTCATGGT TCGAGTGCTA 
GAGAAATCTG ATCAGACCAA CATTTTGAGT GCTTTGCTAG TGCTGCTGCA GGACAGTCTT 
ATCAGCACCG CCGGCTCACC CATGTTCTCT GAACTTGTCA TGAAGTGTCT ATGGAGAATG 
ATCCGTTTCC TACCCCAGAC CATCAACAGT ATTAATCTGG ATCGTATCCT GCTGGACGTG 
CACAATTTCA TGAAGGTTTT CCCTAAAGAG AAGCTGAAGC AACTGAAGAG TGACGTTCCT 
CATCGGACAC TGAAGACACT GCTGCACACG CTCTGCAGAC TCACAGGCGC CAAGATATTA 
GACCACCTGT CCATGATCGA GAACCGCAAT GAGTCAGAGT TGGAAGCGCA TCTGAGGCGA 
GTGGTCAAAC ATTCGGCAAA CCTCTCAGGC CTGAAGTCGG ACAAGGGCAC GGAGAAGGGA 
GCACTCCGAT CGGATGACAA GATGATCAAG GCGAAAGTGA GTGACATCCT GTCTGAGATC 
TTCAAAAAGA TCGGCTCCAA GGAAAACACA AAAGAGGGTT TGACGGAGTT GTACGAGTAC 
AAGCAGAAGT ATTCAGATGC AGACCTGGAG CCCTTCCTAA GGAACACATC TCAGTTCTTT 
CAGAGCTATG TGGAGCGAGG CCTGCGCATG ATCGAGTCTG AGCGTGAGGG AAAGGGCCGC 
ATCCAGACCT CTACAGTGAT CGCGCAGCAC AGCACGGAGT CATTTGTGCC GAGCTCCAGC 
TCTGTGCCCG TCAGCAGTAA TGGAGAAGAT CTAAACGCTG CCGCTTACTA CGAGAGACTG 
AAGATCTTAC GACAGCGGCG CGGGCTGGAG AACTCAGCGC CTGAAGATGA GCGTCCACCC 
CTCAGCTCCC TGAGACCGTC AGTCGCCTCC TCCACAGACA TGCTTCACAG CAAACTATCC 
CAGCTGAAGG AGTCCCGCGA GCACTTCCAG CAGGAGCAGT CGCATTCGCA TAGCCCCACG 
CGCTCCTCCT CACCCGCCTC CAACCTGGAC GACCTCAAGA AGAGGCTGGA GAGGATCAAG 
AGCAACCGCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckap5 ( XM_005174247.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174304.1 Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X2, mRNA.

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CloneID	ODa29089
Clone ID Related Accession (Same CDS sequence)	XM_005174247.3 , XM_005174247.4
Accession Version	XM_005174247.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cytoskeleton-associated protein 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGA GCGAAAGCAT GGAGCTCAAG GTGAAAGGAG AAAAAGATAA TGCTAAGCAA 
AACAGGCTCT CAAGAGGGCC TCCTAGCAGT GAAAAGGTTG TTGCAGGAAA GAAACCTCCT 
GTTAAGGCTG GCGCAAAAGA TGAGGAGGAT AAGTCTGGTC CTATTTTCAT CCTCGTTCCC 
AATGGCAAAG AGCAGAGAAT CAAAGAAGAG AAGTCATTAA AGATTCTGAA GTGGAACTTC 
ATTACTCCTC GTGACGAGTA CGTAGAGCAG CTCAAAACTC AAATGTCAAC ATGTCTGGCC 
AAGTGGCTAC AAGACGAGCT CTTCCACTTC GACTTTCAGC ATCACGTAAA GGCTATCGGA 
GCCATGATTG AGCACATGGA GGCCGAGTCT GAGGCTGTGA TTGGCTGTCT GGACCTGGTG 
TTGAAGTGGT TTACTCTGCG CTTCTTTGAC ACCAACACTA GTGTGATCAT GAAAACCCTG 
GAGTTCCTCA AGATGCTCTT CACCATGCTG AGCAGGAAAA ACTACCAGCT CAATGACTAC 
GAGGCCTCGT CTTTCATTCC TTACCTGATC CTTAAGGTTG GAGAGTCTAA AGATGTTGTG 
CGCAAAGATG TCCGTGCTAT TTTGGCGATG CTGTGTAAAG TCTACGCAGC GAGCAAAGTA 
TTCCCTTACC TTATGGATGG GACCAAATCA AAGAACTCAA AGCAAAGATC TGAATGTCTC 
GAGGAGCTTG GCTGCCTGAT TGAGAACTTC GGTATGAATG TATGCCAACC GACTCCGGCC 
AAGGCTCTGA AAGAAATCGC TGTCCATATT GGAGACCGTG ACACTACAGT CCGCAATGCT 
GCCCTTAATA CTGTCGTAGC GGCCTATAAT GCCTGTGGAG ACCAAGTCTT CAAACTCATC 
GGCAATCTCT CCGAGAAAGA AATGAGCATG CTTGAAGAGA GAATCAAACG TTCGGCCAAG 
AAGACTCCAG CACCCTCAAC CAAACAGGAG CGGCCCCAGA GGGAGCATCC CACAAATCCC 
AATGCAACCT TTCTACGCAA GCCTGCTCAA GAGGAAGTGC CCAACAAGCT TAATCAAGCT 
CGGCAGCAGA ACGCTCACTT GGAGCAGTCG GCTCCATCCA TCCCGAAAGA GTTTCAGCTG 
GATCTGGATG TCTTCGAGAA CAACCACACG TGTGCCAGCG ACATCCCTGA CCTGGTGCAG 
CACAAACTGG ATGAAGTTCT GGAGCCGGTC ATGATTCCTG AGCGCAAAAT TCATTCGGTC 
TCTCCTCATT TTGATGACAT TCACAATAGC ACTGCCTCCA CTATCAACTT TGTCATCTCC 
CAGGTGGCTA GTGGAGACAT TAACACCAGT ATCCAGGCTT TGGCACAGAT TGATGAGGTT 
TTGAGGCAGG CAGACAAAGC AGAAGCCATG TCTGGACACA TCGACCAGTT CCTCATCGCC 
ACCTTCATGC AGCTGCGGCT TATTTACAAC ACACACATGG CTGACGATCG GCTGGACAAG 
AAGGAAATCT TCAAACTCTA CAGTTGCATC ATCGGAAACA TGCTATCTCT GTTCTCGATG 
GAGAGTCTGG CACGGGAGGC CTCGATGGGC GTCCTGAAGG ACCTGATGCA CGGTTTGATC 
ACACTAATGT TGGACTCCAG AGTGGAGGAC ATAGAGGACG GACAGCAGCT CATTCGCTCT 
GTCAACCTGC TCATGGTTCG AGTGCTAGAG AAATCTGATC AGACCAACAT TTTGAGTGCT 
TTGCTAGTGC TGCTGCAGGA CAGTCTTATC AGCACCGCCG GCTCACCCAT GTTCTCTGAA 
CTTGTCATGA AGTGTCTATG GAGAATGATC CGTTTCCTAC CCCAGACCAT CAACAGTATT 
AATCTGGATC GTATCCTGCT GGACGTGCAC AATTTCATGA AGGTTTTCCC TAAAGAGAAG 
CTGAAGCAAC TGAAGAGTGA CGTTCCTCAT CGGACACTGA AGACACTGCT GCACACGCTC 
TGCAGACTCA CAGGCGCCAA GATATTAGAC CACCTGTCCA TGATCGAGAA CCGCAATGAG 
TCAGAGTTGG AAGCGCATCT GAGGCGAGTG GTCAAACATT CGGCAAACCT CTCAGGCCTG 
AAGTCGGACA AGGGCACGGA GAAGGGAGCA CTCCGATCGG ATGACAAGAT GATCAAGGCG 
AAAGTGAGTG ACATCCTGTC TGAGATCTTC AAAAAGATCG GCTCCAAGGA AAACACAAAA 
GAGGGTTTGA CGGAGTTGTA CGAGTACAAG CAGAAGTATT CAGATGCAGA CCTGGAGCCC 
TTCCTAAGGA ACACATCTCA GTTCTTTCAG AGCTATGTGG AGCGAGGCCT GCGCATGATC 
GAGTCTGAGC GTGAGGGAAA GGGCCGCATC CAGACCTCTA CAGTGATCGC GCAGCACAGC 
ACGGAGTCAT TTGTGCCGAG CTCCAGCTCT GTGCCCGTCA GCAGTAATGG AGAAGATCTA 
AACGCTGCCG CTTACTACGA GAGACTGAAG ATCTTACGAC AGCGGCGCGG GCTGGAGAAC 
TCAGCGCCTG AAGATGAGCG TCCACCCCTC AGCTCCCTGA GACCGTCAGT CGCCTCCTCC 
ACAGACATGC TTCACAGCAA ACTATCCCAG CTGAAGGAGT CCCGCGAGCA CTTCCAGCAG 
GAGCAGTCGC ATTCGCATAG CCCCACGCGC TCCTCCTCAC CCGCCTCCAA CCTGGACGAC 
CTCAAGAAGA GGCTGGAGAG GATCAAGAGC AACCGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174304.1
CDS	130..6231
Translation	MGDDSEWMKLPIDQKCEHKVWKARLSGYEEALKLFQKLDEKSPEWSKYLGLIKKFVTESNAVAQLKGLEAALAFIENAHVAGKTVGEVVSGVVNKVFNQPKARAKELGTEICLMYIEIEKAEVVQDELIKGLDNKNPKIVVACIEALRKALCEFGSKIITLKPVVKVLPKLFESREKAVRDEAKLLAVEIYRWIRDALRAPLQNINSVQLKELEEEWVKVPATAPKQIRFLRSQQDLKAKFEQQQAAGGDEADGDDEEVAEAVQVDAYELLEAVEILSKLPKDFYEKIEAKKWQERKEALEAVEALTKNPKLESGDYGDLVRALKKVIGKDANVMLVSMAAKCLAGLATGLRKKFGTYAGLVVPTILEKFKEKKPQVVQALQEAIDAVFLTTTLQNISEDVLAVMDNKNPSIKQQASLFLARSFRHCTPSTLPKSVLKPFCAAFLKQVNDSAPEVRDAAFEALGTAMKVIGEKAVNPFLTDVDKLKLDKIKECADKVELIGKKGGGGGEKKERPAAKASPPVEAPAKTSGPPKKSAPAKAAGPPKKGKPAAAPSAKSKKAPETKEIIETELSPEVCEEKAAAVLPASCMQLLDSANWKERLASMEEFQKAVEQMDKSEMPCQALVKMLAKKPGWKETNFQVMQMKLSIVGLVAQKGQFSKTSALVVLDGLVDKIGDVKCGGNAKEALTAIGEACSLPWTAEQVVSIAFAQKNPKNQAETLNWLANAMKEFGFAGINVKGFINNVKTALGATNPAVRTSAIALLGVMYLYMGAPLRMFFEDEKPALLSQIDAEFVKMQGQSPPAPTRGAKKAGAEEDGDVADEDEADGGAGDIMDMLPRTDISDKITSEMVSKISDKNWKIRKEGLDEVAAVISEAKFIQANIGELPMALKGRLNDSNKLLVQQTLNILQQIAIAMGPSLKQHVKNLGIPVVTVLGDSKPNVRATAMATLNAWVEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLSEKLPTLRTVPADLMLCVPHLYTCLEDRSGDVRKKAQDALPTFMMHLGFDKMTKATGKLKPASKDQVVGMLDKARAVMPAKPAAPAKAAASKPASSAPAAKTASAPARNHSPVDDYSEPEPKPDTKKAKPAGPAAKKSESMELKVKGEKDNAKQNRLSRGPPSSEKVVAGKKPPVKAGAKDEEDKSGPIFILVPNGKEQRIKEEKSLKILKWNFITPRDEYVEQLKTQMSTCLAKWLQDELFHFDFQHHVKAIGAMIEHMEAESEAVIGCLDLVLKWFTLRFFDTNTSVIMKTLEFLKMLFTMLSRKNYQLNDYEASSFIPYLILKVGESKDVVRKDVRAILAMLCKVYAASKVFPYLMDGTKSKNSKQRSECLEELGCLIENFGMNVCQPTPAKALKEIAVHIGDRDTTVRNAALNTVVAAYNACGDQVFKLIGNLSEKEMSMLEERIKRSAKKTPAPSTKQERPQREHPTNPNATFLRKPAQEEVPNKLNQARQQNAHLEQSAPSIPKEFQLDLDVFENNHTCASDIPDLVQHKLDEVLEPVMIPERKIHSVSPHFDDIHNSTASTINFVISQVASGDINTSIQALAQIDEVLRQADKAEAMSGHIDQFLIATFMQLRLIYNTHMADDRLDKKEIFKLYSCIIGNMLSLFSMESLAREASMGVLKDLMHGLITLMLDSRVEDIEDGQQLIRSVNLLMVRVLEKSDQTNILSALLVLLQDSLISTAGSPMFSELVMKCLWRMIRFLPQTINSINLDRILLDVHNFMKVFPKEKLKQLKSDVPHRTLKTLLHTLCRLTGAKILDHLSMIENRNESELEAHLRRVVKHSANLSGLKSDKGTEKGALRSDDKMIKAKVSDILSEIFKKIGSKENTKEGLTELYEYKQKYSDADLEPFLRNTSQFFQSYVERGLRMIESEREGKGRIQTSTVIAQHSTESFVPSSSSVPVSSNGEDLNAAAYYERLKILRQRRGLENSAPEDERPPLSSLRPSVASSTDMLHSKLSQLKESREHFQQEQSHSHSPTRSSSPASNLDDLKKRLERIKSNRQ

Target ORF information:

RefSeq Version	XM_005174247.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174247.3

ORF Insert Sequence:

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGA GCGAAAGCAT GGAGCTCAAG GTGAAAGGAG AAAAAGATAA TGCTAAGCAA 
AACAGGCTCT CAAGAGGGCC TCCTAGCAGT GAAAAGGTTG TTGCAGGAAA GAAACCTCCT 
GTTAAGGCTG GCGCAAAAGA TGAGGAGGAT AAGTCTGGTC CTATTTTCAT CCTCGTTCCC 
AATGGCAAAG AGCAGAGAAT CAAAGAAGAG AAGTCATTAA AGATTCTGAA GTGGAACTTC 
ATTACTCCTC GTGACGAGTA CGTAGAGCAG CTCAAAACTC AAATGTCAAC ATGTCTGGCC 
AAGTGGCTAC AAGACGAGCT CTTCCACTTC GACTTTCAGC ATCACGTAAA GGCTATCGGA 
GCCATGATTG AGCACATGGA GGCCGAGTCT GAGGCTGTGA TTGGCTGTCT GGACCTGGTG 
TTGAAGTGGT TTACTCTGCG CTTCTTTGAC ACCAACACTA GTGTGATCAT GAAAACCCTG 
GAGTTCCTCA AGATGCTCTT CACCATGCTG AGCAGGAAAA ACTACCAGCT CAATGACTAC 
GAGGCCTCGT CTTTCATTCC TTACCTGATC CTTAAGGTTG GAGAGTCTAA AGATGTTGTG 
CGCAAAGATG TCCGTGCTAT TTTGGCGATG CTGTGTAAAG TCTACGCAGC GAGCAAAGTA 
TTCCCTTACC TTATGGATGG GACCAAATCA AAGAACTCAA AGCAAAGATC TGAATGTCTC 
GAGGAGCTTG GCTGCCTGAT TGAGAACTTC GGTATGAATG TATGCCAACC GACTCCGGCC 
AAGGCTCTGA AAGAAATCGC TGTCCATATT GGAGACCGTG ACACTACAGT CCGCAATGCT 
GCCCTTAATA CTGTCGTAGC GGCCTATAAT GCCTGTGGAG ACCAAGTCTT CAAACTCATC 
GGCAATCTCT CCGAGAAAGA AATGAGCATG CTTGAAGAGA GAATCAAACG TTCGGCCAAG 
AAGACTCCAG CACCCTCAAC CAAACAGGAG CGGCCCCAGA GGGAGCATCC CACAAATCCC 
AATGCAACCT TTCTACGCAA GCCTGCTCAA GAGGAAGTGC CCAACAAGCT TAATCAAGCT 
CGGCAGCAGA ACGCTCACTT GGAGCAGTCG GCTCCATCCA TCCCGAAAGA GTTTCAGCTG 
GATCTGGATG TCTTCGAGAA CAACCACACG TGTGCCAGCG ACATCCCTGA CCTGGTGCAG 
CACAAACTGG ATGAAGTTCT GGAGCCGGTC ATGATTCCTG AGCGCAAAAT TCATTCGGTC 
TCTCCTCATT TTGATGACAT TCACAATAGC ACTGCCTCCA CTATCAACTT TGTCATCTCC 
CAGGTGGCTA GTGGAGACAT TAACACCAGT ATCCAGGCTT TGGCACAGAT TGATGAGGTT 
TTGAGGCAGG CAGACAAAGC AGAAGCCATG TCTGGACACA TCGACCAGTT CCTCATCGCC 
ACCTTCATGC AGCTGCGGCT TATTTACAAC ACACACATGG CTGACGATCG GCTGGACAAG 
AAGGAAATCT TCAAACTCTA CAGTTGCATC ATCGGAAACA TGCTATCTCT GTTCTCGATG 
GAGAGTCTGG CACGGGAGGC CTCGATGGGC GTCCTGAAGG ACCTGATGCA CGGTTTGATC 
ACACTAATGT TGGACTCCAG AGTGGAGGAC ATAGAGGACG GACAGCAGCT CATTCGCTCT 
GTCAACCTGC TCATGGTTCG AGTGCTAGAG AAATCTGATC AGACCAACAT TTTGAGTGCT 
TTGCTAGTGC TGCTGCAGGA CAGTCTTATC AGCACCGCCG GCTCACCCAT GTTCTCTGAA 
CTTGTCATGA AGTGTCTATG GAGAATGATC CGTTTCCTAC CCCAGACCAT CAACAGTATT 
AATCTGGATC GTATCCTGCT GGACGTGCAC AATTTCATGA AGGTTTTCCC TAAAGAGAAG 
CTGAAGCAAC TGAAGAGTGA CGTTCCTCAT CGGACACTGA AGACACTGCT GCACACGCTC 
TGCAGACTCA CAGGCGCCAA GATATTAGAC CACCTGTCCA TGATCGAGAA CCGCAATGAG 
TCAGAGTTGG AAGCGCATCT GAGGCGAGTG GTCAAACATT CGGCAAACCT CTCAGGCCTG 
AAGTCGGACA AGGGCACGGA GAAGGGAGCA CTCCGATCGG ATGACAAGAT GATCAAGGCG 
AAAGTGAGTG ACATCCTGTC TGAGATCTTC AAAAAGATCG GCTCCAAGGA AAACACAAAA 
GAGGGTTTGA CGGAGTTGTA CGAGTACAAG CAGAAGTATT CAGATGCAGA CCTGGAGCCC 
TTCCTAAGGA ACACATCTCA GTTCTTTCAG AGCTATGTGG AGCGAGGCCT GCGCATGATC 
GAGTCTGAGC GTGAGGGAAA GGGCCGCATC CAGACCTCTA CAGTGATCGC GCAGCACAGC 
ACGGAGTCAT TTGTGCCGAG CTCCAGCTCT GTGCCCGTCA GCAGTAATGG AGAAGATCTA 
AACGCTGCCG CTTACTACGA GAGACTGAAG ATCTTACGAC AGCGGCGCGG GCTGGAGAAC 
TCAGCGCCTG AAGATGAGCG TCCACCCCTC AGCTCCCTGA GACCGTCAGT CGCCTCCTCC 
ACAGACATGC TTCACAGCAA ACTATCCCAG CTGAAGGAGT CCCGCGAGCA CTTCCAGCAG 
GAGCAGTCGC ATTCGCATAG CCCCACGCGC TCCTCCTCAC CCGCCTCCAA CCTGGACGAC 
CTCAAGAAGA GGCTGGAGAG GATCAAGAGC AACCGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckap5 ( XM_005174247.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174304.1 Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X2, mRNA.

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CloneID	ODa29089
Clone ID Related Accession (Same CDS sequence)	XM_005174247.3 , XM_005174247.4
Accession Version	XM_005174247.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cytoskeleton-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174247.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGA GCGAAAGCAT GGAGCTCAAG GTGAAAGGAG AAAAAGATAA TGCTAAGCAA 
AACAGGCTCT CAAGAGGGCC TCCTAGCAGT GAAAAGGTTG TTGCAGGAAA GAAACCTCCT 
GTTAAGGCTG GCGCAAAAGA TGAGGAGGAT AAGTCTGGTC CTATTTTCAT CCTCGTTCCC 
AATGGCAAAG AGCAGAGAAT CAAAGAAGAG AAGTCATTAA AGATTCTGAA GTGGAACTTC 
ATTACTCCTC GTGACGAGTA CGTAGAGCAG CTCAAAACTC AAATGTCAAC ATGTCTGGCC 
AAGTGGCTAC AAGACGAGCT CTTCCACTTC GACTTTCAGC ATCACGTAAA GGCTATCGGA 
GCCATGATTG AGCACATGGA GGCCGAGTCT GAGGCTGTGA TTGGCTGTCT GGACCTGGTG 
TTGAAGTGGT TTACTCTGCG CTTCTTTGAC ACCAACACTA GTGTGATCAT GAAAACCCTG 
GAGTTCCTCA AGATGCTCTT CACCATGCTG AGCAGGAAAA ACTACCAGCT CAATGACTAC 
GAGGCCTCGT CTTTCATTCC TTACCTGATC CTTAAGGTTG GAGAGTCTAA AGATGTTGTG 
CGCAAAGATG TCCGTGCTAT TTTGGCGATG CTGTGTAAAG TCTACGCAGC GAGCAAAGTA 
TTCCCTTACC TTATGGATGG GACCAAATCA AAGAACTCAA AGCAAAGATC TGAATGTCTC 
GAGGAGCTTG GCTGCCTGAT TGAGAACTTC GGTATGAATG TATGCCAACC GACTCCGGCC 
AAGGCTCTGA AAGAAATCGC TGTCCATATT GGAGACCGTG ACACTACAGT CCGCAATGCT 
GCCCTTAATA CTGTCGTAGC GGCCTATAAT GCCTGTGGAG ACCAAGTCTT CAAACTCATC 
GGCAATCTCT CCGAGAAAGA AATGAGCATG CTTGAAGAGA GAATCAAACG TTCGGCCAAG 
AAGACTCCAG CACCCTCAAC CAAACAGGAG CGGCCCCAGA GGGAGCATCC CACAAATCCC 
AATGCAACCT TTCTACGCAA GCCTGCTCAA GAGGAAGTGC CCAACAAGCT TAATCAAGCT 
CGGCAGCAGA ACGCTCACTT GGAGCAGTCG GCTCCATCCA TCCCGAAAGA GTTTCAGCTG 
GATCTGGATG TCTTCGAGAA CAACCACACG TGTGCCAGCG ACATCCCTGA CCTGGTGCAG 
CACAAACTGG ATGAAGTTCT GGAGCCGGTC ATGATTCCTG AGCGCAAAAT TCATTCGGTC 
TCTCCTCATT TTGATGACAT TCACAATAGC ACTGCCTCCA CTATCAACTT TGTCATCTCC 
CAGGTGGCTA GTGGAGACAT TAACACCAGT ATCCAGGCTT TGGCACAGAT TGATGAGGTT 
TTGAGGCAGG CAGACAAAGC AGAAGCCATG TCTGGACACA TCGACCAGTT CCTCATCGCC 
ACCTTCATGC AGCTGCGGCT TATTTACAAC ACACACATGG CTGACGATCG GCTGGACAAG 
AAGGAAATCT TCAAACTCTA CAGTTGCATC ATCGGAAACA TGCTATCTCT GTTCTCGATG 
GAGAGTCTGG CACGGGAGGC CTCGATGGGC GTCCTGAAGG ACCTGATGCA CGGTTTGATC 
ACACTAATGT TGGACTCCAG AGTGGAGGAC ATAGAGGACG GACAGCAGCT CATTCGCTCT 
GTCAACCTGC TCATGGTTCG AGTGCTAGAG AAATCTGATC AGACCAACAT TTTGAGTGCT 
TTGCTAGTGC TGCTGCAGGA CAGTCTTATC AGCACCGCCG GCTCACCCAT GTTCTCTGAA 
CTTGTCATGA AGTGTCTATG GAGAATGATC CGTTTCCTAC CCCAGACCAT CAACAGTATT 
AATCTGGATC GTATCCTGCT GGACGTGCAC AATTTCATGA AGGTTTTCCC TAAAGAGAAG 
CTGAAGCAAC TGAAGAGTGA CGTTCCTCAT CGGACACTGA AGACACTGCT GCACACGCTC 
TGCAGACTCA CAGGCGCCAA GATATTAGAC CACCTGTCCA TGATCGAGAA CCGCAATGAG 
TCAGAGTTGG AAGCGCATCT GAGGCGAGTG GTCAAACATT CGGCAAACCT CTCAGGCCTG 
AAGTCGGACA AGGGCACGGA GAAGGGAGCA CTCCGATCGG ATGACAAGAT GATCAAGGCG 
AAAGTGAGTG ACATCCTGTC TGAGATCTTC AAAAAGATCG GCTCCAAGGA AAACACAAAA 
GAGGGTTTGA CGGAGTTGTA CGAGTACAAG CAGAAGTATT CAGATGCAGA CCTGGAGCCC 
TTCCTAAGGA ACACATCTCA GTTCTTTCAG AGCTATGTGG AGCGAGGCCT GCGCATGATC 
GAGTCTGAGC GTGAGGGAAA GGGCCGCATC CAGACCTCTA CAGTGATCGC GCAGCACAGC 
ACGGAGTCAT TTGTGCCGAG CTCCAGCTCT GTGCCCGTCA GCAGTAATGG AGAAGATCTA 
AACGCTGCCG CTTACTACGA GAGACTGAAG ATCTTACGAC AGCGGCGCGG GCTGGAGAAC 
TCAGCGCCTG AAGATGAGCG TCCACCCCTC AGCTCCCTGA GACCGTCAGT CGCCTCCTCC 
ACAGACATGC TTCACAGCAA ACTATCCCAG CTGAAGGAGT CCCGCGAGCA CTTCCAGCAG 
GAGCAGTCGC ATTCGCATAG CCCCACGCGC TCCTCCTCAC CCGCCTCCAA CCTGGACGAC 
CTCAAGAAGA GGCTGGAGAG GATCAAGAGC AACCGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174304.1
CDS	162..6263
Translation	MGDDSEWMKLPIDQKCEHKVWKARLSGYEEALKLFQKLDEKSPEWSKYLGLIKKFVTESNAVAQLKGLEAALAFIENAHVAGKTVGEVVSGVVNKVFNQPKARAKELGTEICLMYIEIEKAEVVQDELIKGLDNKNPKIVVACIEALRKALCEFGSKIITLKPVVKVLPKLFESREKAVRDEAKLLAVEIYRWIRDALRAPLQNINSVQLKELEEEWVKVPATAPKQIRFLRSQQDLKAKFEQQQAAGGDEADGDDEEVAEAVQVDAYELLEAVEILSKLPKDFYEKIEAKKWQERKEALEAVEALTKNPKLESGDYGDLVRALKKVIGKDANVMLVSMAAKCLAGLATGLRKKFGTYAGLVVPTILEKFKEKKPQVVQALQEAIDAVFLTTTLQNISEDVLAVMDNKNPSIKQQASLFLARSFRHCTPSTLPKSVLKPFCAAFLKQVNDSAPEVRDAAFEALGTAMKVIGEKAVNPFLTDVDKLKLDKIKECADKVELIGKKGGGGGEKKERPAAKASPPVEAPAKTSGPPKKSAPAKAAGPPKKGKPAAAPSAKSKKAPETKEIIETELSPEVCEEKAAAVLPASCMQLLDSANWKERLASMEEFQKAVEQMDKSEMPCQALVKMLAKKPGWKETNFQVMQMKLSIVGLVAQKGQFSKTSALVVLDGLVDKIGDVKCGGNAKEALTAIGEACSLPWTAEQVVSIAFAQKNPKNQAETLNWLANAMKEFGFAGINVKGFINNVKTALGATNPAVRTSAIALLGVMYLYMGAPLRMFFEDEKPALLSQIDAEFVKMQGQSPPAPTRGAKKAGAEEDGDVADEDEADGGAGDIMDMLPRTDISDKITSEMVSKISDKNWKIRKEGLDEVAAVISEAKFIQANIGELPMALKGRLNDSNKLLVQQTLNILQQIAIAMGPSLKQHVKNLGIPVVTVLGDSKPNVRATAMATLNAWVEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLSEKLPTLRTVPADLMLCVPHLYTCLEDRSGDVRKKAQDALPTFMMHLGFDKMTKATGKLKPASKDQVVGMLDKARAVMPAKPAAPAKAAASKPASSAPAAKTASAPARNHSPVDDYSEPEPKPDTKKAKPAGPAAKKSESMELKVKGEKDNAKQNRLSRGPPSSEKVVAGKKPPVKAGAKDEEDKSGPIFILVPNGKEQRIKEEKSLKILKWNFITPRDEYVEQLKTQMSTCLAKWLQDELFHFDFQHHVKAIGAMIEHMEAESEAVIGCLDLVLKWFTLRFFDTNTSVIMKTLEFLKMLFTMLSRKNYQLNDYEASSFIPYLILKVGESKDVVRKDVRAILAMLCKVYAASKVFPYLMDGTKSKNSKQRSECLEELGCLIENFGMNVCQPTPAKALKEIAVHIGDRDTTVRNAALNTVVAAYNACGDQVFKLIGNLSEKEMSMLEERIKRSAKKTPAPSTKQERPQREHPTNPNATFLRKPAQEEVPNKLNQARQQNAHLEQSAPSIPKEFQLDLDVFENNHTCASDIPDLVQHKLDEVLEPVMIPERKIHSVSPHFDDIHNSTASTINFVISQVASGDINTSIQALAQIDEVLRQADKAEAMSGHIDQFLIATFMQLRLIYNTHMADDRLDKKEIFKLYSCIIGNMLSLFSMESLAREASMGVLKDLMHGLITLMLDSRVEDIEDGQQLIRSVNLLMVRVLEKSDQTNILSALLVLLQDSLISTAGSPMFSELVMKCLWRMIRFLPQTINSINLDRILLDVHNFMKVFPKEKLKQLKSDVPHRTLKTLLHTLCRLTGAKILDHLSMIENRNESELEAHLRRVVKHSANLSGLKSDKGTEKGALRSDDKMIKAKVSDILSEIFKKIGSKENTKEGLTELYEYKQKYSDADLEPFLRNTSQFFQSYVERGLRMIESEREGKGRIQTSTVIAQHSTESFVPSSSSVPVSSNGEDLNAAAYYERLKILRQRRGLENSAPEDERPPLSSLRPSVASSTDMLHSKLSQLKESREHFQQEQSHSHSPTRSSSPASNLDDLKKRLERIKSNRQ

Target ORF information:

RefSeq Version	XM_005174247.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174247.4

ORF Insert Sequence:

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGA GCGAAAGCAT GGAGCTCAAG GTGAAAGGAG AAAAAGATAA TGCTAAGCAA 
AACAGGCTCT CAAGAGGGCC TCCTAGCAGT GAAAAGGTTG TTGCAGGAAA GAAACCTCCT 
GTTAAGGCTG GCGCAAAAGA TGAGGAGGAT AAGTCTGGTC CTATTTTCAT CCTCGTTCCC 
AATGGCAAAG AGCAGAGAAT CAAAGAAGAG AAGTCATTAA AGATTCTGAA GTGGAACTTC 
ATTACTCCTC GTGACGAGTA CGTAGAGCAG CTCAAAACTC AAATGTCAAC ATGTCTGGCC 
AAGTGGCTAC AAGACGAGCT CTTCCACTTC GACTTTCAGC ATCACGTAAA GGCTATCGGA 
GCCATGATTG AGCACATGGA GGCCGAGTCT GAGGCTGTGA TTGGCTGTCT GGACCTGGTG 
TTGAAGTGGT TTACTCTGCG CTTCTTTGAC ACCAACACTA GTGTGATCAT GAAAACCCTG 
GAGTTCCTCA AGATGCTCTT CACCATGCTG AGCAGGAAAA ACTACCAGCT CAATGACTAC 
GAGGCCTCGT CTTTCATTCC TTACCTGATC CTTAAGGTTG GAGAGTCTAA AGATGTTGTG 
CGCAAAGATG TCCGTGCTAT TTTGGCGATG CTGTGTAAAG TCTACGCAGC GAGCAAAGTA 
TTCCCTTACC TTATGGATGG GACCAAATCA AAGAACTCAA AGCAAAGATC TGAATGTCTC 
GAGGAGCTTG GCTGCCTGAT TGAGAACTTC GGTATGAATG TATGCCAACC GACTCCGGCC 
AAGGCTCTGA AAGAAATCGC TGTCCATATT GGAGACCGTG ACACTACAGT CCGCAATGCT 
GCCCTTAATA CTGTCGTAGC GGCCTATAAT GCCTGTGGAG ACCAAGTCTT CAAACTCATC 
GGCAATCTCT CCGAGAAAGA AATGAGCATG CTTGAAGAGA GAATCAAACG TTCGGCCAAG 
AAGACTCCAG CACCCTCAAC CAAACAGGAG CGGCCCCAGA GGGAGCATCC CACAAATCCC 
AATGCAACCT TTCTACGCAA GCCTGCTCAA GAGGAAGTGC CCAACAAGCT TAATCAAGCT 
CGGCAGCAGA ACGCTCACTT GGAGCAGTCG GCTCCATCCA TCCCGAAAGA GTTTCAGCTG 
GATCTGGATG TCTTCGAGAA CAACCACACG TGTGCCAGCG ACATCCCTGA CCTGGTGCAG 
CACAAACTGG ATGAAGTTCT GGAGCCGGTC ATGATTCCTG AGCGCAAAAT TCATTCGGTC 
TCTCCTCATT TTGATGACAT TCACAATAGC ACTGCCTCCA CTATCAACTT TGTCATCTCC 
CAGGTGGCTA GTGGAGACAT TAACACCAGT ATCCAGGCTT TGGCACAGAT TGATGAGGTT 
TTGAGGCAGG CAGACAAAGC AGAAGCCATG TCTGGACACA TCGACCAGTT CCTCATCGCC 
ACCTTCATGC AGCTGCGGCT TATTTACAAC ACACACATGG CTGACGATCG GCTGGACAAG 
AAGGAAATCT TCAAACTCTA CAGTTGCATC ATCGGAAACA TGCTATCTCT GTTCTCGATG 
GAGAGTCTGG CACGGGAGGC CTCGATGGGC GTCCTGAAGG ACCTGATGCA CGGTTTGATC 
ACACTAATGT TGGACTCCAG AGTGGAGGAC ATAGAGGACG GACAGCAGCT CATTCGCTCT 
GTCAACCTGC TCATGGTTCG AGTGCTAGAG AAATCTGATC AGACCAACAT TTTGAGTGCT 
TTGCTAGTGC TGCTGCAGGA CAGTCTTATC AGCACCGCCG GCTCACCCAT GTTCTCTGAA 
CTTGTCATGA AGTGTCTATG GAGAATGATC CGTTTCCTAC CCCAGACCAT CAACAGTATT 
AATCTGGATC GTATCCTGCT GGACGTGCAC AATTTCATGA AGGTTTTCCC TAAAGAGAAG 
CTGAAGCAAC TGAAGAGTGA CGTTCCTCAT CGGACACTGA AGACACTGCT GCACACGCTC 
TGCAGACTCA CAGGCGCCAA GATATTAGAC CACCTGTCCA TGATCGAGAA CCGCAATGAG 
TCAGAGTTGG AAGCGCATCT GAGGCGAGTG GTCAAACATT CGGCAAACCT CTCAGGCCTG 
AAGTCGGACA AGGGCACGGA GAAGGGAGCA CTCCGATCGG ATGACAAGAT GATCAAGGCG 
AAAGTGAGTG ACATCCTGTC TGAGATCTTC AAAAAGATCG GCTCCAAGGA AAACACAAAA 
GAGGGTTTGA CGGAGTTGTA CGAGTACAAG CAGAAGTATT CAGATGCAGA CCTGGAGCCC 
TTCCTAAGGA ACACATCTCA GTTCTTTCAG AGCTATGTGG AGCGAGGCCT GCGCATGATC 
GAGTCTGAGC GTGAGGGAAA GGGCCGCATC CAGACCTCTA CAGTGATCGC GCAGCACAGC 
ACGGAGTCAT TTGTGCCGAG CTCCAGCTCT GTGCCCGTCA GCAGTAATGG AGAAGATCTA 
AACGCTGCCG CTTACTACGA GAGACTGAAG ATCTTACGAC AGCGGCGCGG GCTGGAGAAC 
TCAGCGCCTG AAGATGAGCG TCCACCCCTC AGCTCCCTGA GACCGTCAGT CGCCTCCTCC 
ACAGACATGC TTCACAGCAA ACTATCCCAG CTGAAGGAGT CCCGCGAGCA CTTCCAGCAG 
GAGCAGTCGC ATTCGCATAG CCCCACGCGC TCCTCCTCAC CCGCCTCCAA CCTGGACGAC 
CTCAAGAAGA GGCTGGAGAG GATCAAGAGC AACCGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckap5 ( XM_009297901.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296176.1 Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X1, mRNA.

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CloneID	ODa41390
Clone ID Related Accession (Same CDS sequence)	XM_009297901.2
Accession Version	XM_009297901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cytoskeleton-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGA GCGAAAGCAT GGAGCTCAAG GTGAAAGGAG AAAAAGATAA TGCTAAGCAA 
AACAGGCTCT CAAGAGGGCC TCCTAGCAGT GAAAAGGTTG TTGCAGGAAA GAAACCTCCT 
GTTAAGGCTG GCGCAAAAGA TGAGGAGGAT AAGTCTGGTC CTATTTTCAT CCTCGTTCCC 
AATGGCAAAG AGCAGAGAAT CAAAGAAGAG AAGTCATTAA AGATTCTGAA GTGGAACTTC 
ATTACTCCTC GTGACGAGTA CGTAGAGCAG CTCAAAACTC AAATGTCAAC ATGTCTGGCC 
AAGTGGCTAC AAGACGAGCT CTTCCACTTC GACTTTCAGC ATCACGTAAA GGCTATCGGA 
GCCATGATTG AGCACATGGA GGCCGAGTCT GAGGCTGTGA TTGGCTGTCT GGACCTGGTG 
TTGAAGTGGT TTACTCTGCG CTTCTTTGAC ACCAACACTA GTGTGATCAT GAAAACCCTG 
GAGTTCCTCA AGATGCTCTT CACCATGCTG AGCAGGAAAA ACTACCAGCT CAATGACTAC 
GAGGCCTCGT CTTTCATTCC TTACCTGATC CTTAAGGTTG GAGAGTCTAA AGATGTTGTG 
CGCAAAGATG TCCGTGCTAT TTTGGCGATG CTGTGTAAAG TCTACGCAGC GAGCAAAGTA 
TTCCCTTACC TTATGGATGG GACCAAATCA AAGAACTCAA AGCAAAGATC TGAATGTCTC 
GAGGAGCTTG GCTGCCTGAT TGAGAACTTC GGTATGAATG TATGCCAACC GACTCCGGCC 
AAGGCTCTGA AAGAAATCGC TGTCCATATT GGAGACCGTG ACACTACAGT CCGCAATGCT 
GCCCTTAATA CTGTCGTAGC GGCCTATAAT GCCTGTGGAG ACCAAGTCTT CAAACTCATC 
GGCAATCTCT CCGAGAAAGA AATGAGCATG CTTGAAGAGA GAATCAAACG TTCGGCCAAG 
AAGACTCCAG CACCCTCAAC CAAACAGGAG CGGCCCCAGA GGGAGCATCC CACAAATCCC 
AATGCAACCT TTCTACGCAA GCCTGCTCAA GAGGAAGTGC CCAACAAGCT TAAAATAATG 
TATCGCACTT ACAGGATTCA AGCTCGGCAG CAGAACGCTC ACTTGGAGCA GTCGGCTCCA 
TCCATCCCGA AAGAGTTTCA GCTGGATCTG GATGTCTTCG AGAACAACCA CACGTGTGCC 
AGCGACATCC CTGACCTGGT GCAGCACAAA CTGGATGAAG TTCTGGAGCC GGTCATGATT 
CCTGAGCGCA AAATTCATTC GGTCTCTCCT CATTTTGATG ACATTCACAA TAGCACTGCC 
TCCACTATCA ACTTTGTCAT CTCCCAGGTG GCTAGTGGAG ACATTAACAC CAGTATCCAG 
GCTTTGGCAC AGATTGATGA GGTTTTGAGG CAGGCAGACA AAGCAGAAGC CATGTCTGGA 
CACATCGACC AGTTCCTCAT CGCCACCTTC ATGCAGCTGC GGCTTATTTA CAACACACAC 
ATGGCTGACG ATCGGCTGGA CAAGAAGGAA ATCTTCAAAC TCTACAGTTG CATCATCGGA 
AACATGCTAT CTCTGTTCTC GATGGAGAGT CTGGCACGGG AGGCCTCGAT GGGCGTCCTG 
AAGGACCTGA TGCACGGTTT GATCACACTA ATGTTGGACT CCAGAGTGGA GGACATAGAG 
GACGGACAGC AGCTCATTCG CTCTGTCAAC CTGCTCATGG TTCGAGTGCT AGAGAAATCT 
GATCAGACCA ACATTTTGAG TGCTTTGCTA GTGCTGCTGC AGGACAGTCT TATCAGCACC 
GCCGGCTCAC CCATGTTCTC TGAACTTGTC ATGAAGTGTC TATGGAGAAT GATCCGTTTC 
CTACCCCAGA CCATCAACAG TATTAATCTG GATCGTATCC TGCTGGACGT GCACAATTTC 
ATGAAGGTTT TCCCTAAAGA GAAGCTGAAG CAACTGAAGA GTGACGTTCC TCATCGGACA 
CTGAAGACAC TGCTGCACAC GCTCTGCAGA CTCACAGGCG CCAAGATATT AGACCACCTG 
TCCATGATCG AGAACCGCAA TGAGTCAGAG TTGGAAGCGC ATCTGAGGCG AGTGGTCAAA 
CATTCGGCAA ACCTCTCAGG CCTGAAGTCG GACAAGGGCA CGGAGAAGGG AGCACTCCGA 
TCGGATGACA AGATGATCAA GGCGAAAGTG AGTGACATCC TGTCTGAGAT CTTCAAAAAG 
ATCGGCTCCA AGGAAAACAC AAAAGAGGGT TTGACGGAGT TGTACGAGTA CAAGCAGAAG 
TATTCAGATG CAGACCTGGA GCCCTTCCTA AGGAACACAT CTCAGTTCTT TCAGAGCTAT 
GTGGAGCGAG GCCTGCGCAT GATCGAGTCT GAGCGTGAGG GAAAGGGCCG CATCCAGACC 
TCTACAGTGA TCGCGCAGCA CAGCACGGAG TCATTTGTGC CGAGCTCCAG CTCTGTGCCC 
GTCAGCAGTA ATGGAGAAGA TCTAAACGCT GCCGCTTACT ACGAGAGACT GAAGATCTTA 
CGACAGCGGC GCGGGCTGGA GAACTCAGCG CCTGAAGATG AGCGTCCACC CCTCAGCTCC 
CTGAGACCGT CAGTCGCCTC CTCCACAGAC ATGCTTCACA GCAAACTATC CCAGCTGAAG 
GAGTCCCGCG AGCACTTCCA GCAGGAGCAG TCGCATTCGC ATAGCCCCAC GCGCTCCTCC 
TCACCCGCCT CCAACCTGGA CGACCTCAAG AAGAGGCTGG AGAGGATCAA GAGCAACCGC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296176.1
CDS	185..6310
Translation	MGDDSEWMKLPIDQKCEHKVWKARLSGYEEALKLFQKLDEKSPEWSKYLGLIKKFVTESNAVAQLKGLEAALAFIENAHVAGKTVGEVVSGVVNKVFNQPKARAKELGTEICLMYIEIEKAEVVQDELIKGLDNKNPKIVVACIEALRKALCEFGSKIITLKPVVKVLPKLFESREKAVRDEAKLLAVEIYRWIRDALRAPLQNINSVQLKELEEEWVKVPATAPKQIRFLRSQQDLKAKFEQQQAAGGDEADGDDEEVAEAVQVDAYELLEAVEILSKLPKDFYEKIEAKKWQERKEALEAVEALTKNPKLESGDYGDLVRALKKVIGKDANVMLVSMAAKCLAGLATGLRKKFGTYAGLVVPTILEKFKEKKPQVVQALQEAIDAVFLTTTLQNISEDVLAVMDNKNPSIKQQASLFLARSFRHCTPSTLPKSVLKPFCAAFLKQVNDSAPEVRDAAFEALGTAMKVIGEKAVNPFLTDVDKLKLDKIKECADKVELIGKKGGGGGEKKERPAAKASPPVEAPAKTSGPPKKSAPAKAAGPPKKGKPAAAPSAKSKKAPETKEIIETELSPEVCEEKAAAVLPASCMQLLDSANWKERLASMEEFQKAVEQMDKSEMPCQALVKMLAKKPGWKETNFQVMQMKLSIVGLVAQKGQFSKTSALVVLDGLVDKIGDVKCGGNAKEALTAIGEACSLPWTAEQVVSIAFAQKNPKNQAETLNWLANAMKEFGFAGINVKGFINNVKTALGATNPAVRTSAIALLGVMYLYMGAPLRMFFEDEKPALLSQIDAEFVKMQGQSPPAPTRGAKKAGAEEDGDVADEDEADGGAGDIMDMLPRTDISDKITSEMVSKISDKNWKIRKEGLDEVAAVISEAKFIQANIGELPMALKGRLNDSNKLLVQQTLNILQQIAIAMGPSLKQHVKNLGIPVVTVLGDSKPNVRATAMATLNAWVEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLSEKLPTLRTVPADLMLCVPHLYTCLEDRSGDVRKKAQDALPTFMMHLGFDKMTKATGKLKPASKDQVVGMLDKARAVMPAKPAAPAKAAASKPASSAPAAKTASAPARNHSPVDDYSEPEPKPDTKKAKPAGPAAKKSESMELKVKGEKDNAKQNRLSRGPPSSEKVVAGKKPPVKAGAKDEEDKSGPIFILVPNGKEQRIKEEKSLKILKWNFITPRDEYVEQLKTQMSTCLAKWLQDELFHFDFQHHVKAIGAMIEHMEAESEAVIGCLDLVLKWFTLRFFDTNTSVIMKTLEFLKMLFTMLSRKNYQLNDYEASSFIPYLILKVGESKDVVRKDVRAILAMLCKVYAASKVFPYLMDGTKSKNSKQRSECLEELGCLIENFGMNVCQPTPAKALKEIAVHIGDRDTTVRNAALNTVVAAYNACGDQVFKLIGNLSEKEMSMLEERIKRSAKKTPAPSTKQERPQREHPTNPNATFLRKPAQEEVPNKLKIMYRTYRIQARQQNAHLEQSAPSIPKEFQLDLDVFENNHTCASDIPDLVQHKLDEVLEPVMIPERKIHSVSPHFDDIHNSTASTINFVISQVASGDINTSIQALAQIDEVLRQADKAEAMSGHIDQFLIATFMQLRLIYNTHMADDRLDKKEIFKLYSCIIGNMLSLFSMESLAREASMGVLKDLMHGLITLMLDSRVEDIEDGQQLIRSVNLLMVRVLEKSDQTNILSALLVLLQDSLISTAGSPMFSELVMKCLWRMIRFLPQTINSINLDRILLDVHNFMKVFPKEKLKQLKSDVPHRTLKTLLHTLCRLTGAKILDHLSMIENRNESELEAHLRRVVKHSANLSGLKSDKGTEKGALRSDDKMIKAKVSDILSEIFKKIGSKENTKEGLTELYEYKQKYSDADLEPFLRNTSQFFQSYVERGLRMIESEREGKGRIQTSTVIAQHSTESFVPSSSSVPVSSNGEDLNAAAYYERLKILRQRRGLENSAPEDERPPLSSLRPSVASSTDMLHSKLSQLKESREHFQQEQSHSHSPTRSSSPASNLDDLKKRLERIKSNRQ

Target ORF information:

RefSeq Version	XM_009297901.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297901.2

ORF Insert Sequence:

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGA GCGAAAGCAT GGAGCTCAAG GTGAAAGGAG AAAAAGATAA TGCTAAGCAA 
AACAGGCTCT CAAGAGGGCC TCCTAGCAGT GAAAAGGTTG TTGCAGGAAA GAAACCTCCT 
GTTAAGGCTG GCGCAAAAGA TGAGGAGGAT AAGTCTGGTC CTATTTTCAT CCTCGTTCCC 
AATGGCAAAG AGCAGAGAAT CAAAGAAGAG AAGTCATTAA AGATTCTGAA GTGGAACTTC 
ATTACTCCTC GTGACGAGTA CGTAGAGCAG CTCAAAACTC AAATGTCAAC ATGTCTGGCC 
AAGTGGCTAC AAGACGAGCT CTTCCACTTC GACTTTCAGC ATCACGTAAA GGCTATCGGA 
GCCATGATTG AGCACATGGA GGCCGAGTCT GAGGCTGTGA TTGGCTGTCT GGACCTGGTG 
TTGAAGTGGT TTACTCTGCG CTTCTTTGAC ACCAACACTA GTGTGATCAT GAAAACCCTG 
GAGTTCCTCA AGATGCTCTT CACCATGCTG AGCAGGAAAA ACTACCAGCT CAATGACTAC 
GAGGCCTCGT CTTTCATTCC TTACCTGATC CTTAAGGTTG GAGAGTCTAA AGATGTTGTG 
CGCAAAGATG TCCGTGCTAT TTTGGCGATG CTGTGTAAAG TCTACGCAGC GAGCAAAGTA 
TTCCCTTACC TTATGGATGG GACCAAATCA AAGAACTCAA AGCAAAGATC TGAATGTCTC 
GAGGAGCTTG GCTGCCTGAT TGAGAACTTC GGTATGAATG TATGCCAACC GACTCCGGCC 
AAGGCTCTGA AAGAAATCGC TGTCCATATT GGAGACCGTG ACACTACAGT CCGCAATGCT 
GCCCTTAATA CTGTCGTAGC GGCCTATAAT GCCTGTGGAG ACCAAGTCTT CAAACTCATC 
GGCAATCTCT CCGAGAAAGA AATGAGCATG CTTGAAGAGA GAATCAAACG TTCGGCCAAG 
AAGACTCCAG CACCCTCAAC CAAACAGGAG CGGCCCCAGA GGGAGCATCC CACAAATCCC 
AATGCAACCT TTCTACGCAA GCCTGCTCAA GAGGAAGTGC CCAACAAGCT TAAAATAATG 
TATCGCACTT ACAGGATTCA AGCTCGGCAG CAGAACGCTC ACTTGGAGCA GTCGGCTCCA 
TCCATCCCGA AAGAGTTTCA GCTGGATCTG GATGTCTTCG AGAACAACCA CACGTGTGCC 
AGCGACATCC CTGACCTGGT GCAGCACAAA CTGGATGAAG TTCTGGAGCC GGTCATGATT 
CCTGAGCGCA AAATTCATTC GGTCTCTCCT CATTTTGATG ACATTCACAA TAGCACTGCC 
TCCACTATCA ACTTTGTCAT CTCCCAGGTG GCTAGTGGAG ACATTAACAC CAGTATCCAG 
GCTTTGGCAC AGATTGATGA GGTTTTGAGG CAGGCAGACA AAGCAGAAGC CATGTCTGGA 
CACATCGACC AGTTCCTCAT CGCCACCTTC ATGCAGCTGC GGCTTATTTA CAACACACAC 
ATGGCTGACG ATCGGCTGGA CAAGAAGGAA ATCTTCAAAC TCTACAGTTG CATCATCGGA 
AACATGCTAT CTCTGTTCTC GATGGAGAGT CTGGCACGGG AGGCCTCGAT GGGCGTCCTG 
AAGGACCTGA TGCACGGTTT GATCACACTA ATGTTGGACT CCAGAGTGGA GGACATAGAG 
GACGGACAGC AGCTCATTCG CTCTGTCAAC CTGCTCATGG TTCGAGTGCT AGAGAAATCT 
GATCAGACCA ACATTTTGAG TGCTTTGCTA GTGCTGCTGC AGGACAGTCT TATCAGCACC 
GCCGGCTCAC CCATGTTCTC TGAACTTGTC ATGAAGTGTC TATGGAGAAT GATCCGTTTC 
CTACCCCAGA CCATCAACAG TATTAATCTG GATCGTATCC TGCTGGACGT GCACAATTTC 
ATGAAGGTTT TCCCTAAAGA GAAGCTGAAG CAACTGAAGA GTGACGTTCC TCATCGGACA 
CTGAAGACAC TGCTGCACAC GCTCTGCAGA CTCACAGGCG CCAAGATATT AGACCACCTG 
TCCATGATCG AGAACCGCAA TGAGTCAGAG TTGGAAGCGC ATCTGAGGCG AGTGGTCAAA 
CATTCGGCAA ACCTCTCAGG CCTGAAGTCG GACAAGGGCA CGGAGAAGGG AGCACTCCGA 
TCGGATGACA AGATGATCAA GGCGAAAGTG AGTGACATCC TGTCTGAGAT CTTCAAAAAG 
ATCGGCTCCA AGGAAAACAC AAAAGAGGGT TTGACGGAGT TGTACGAGTA CAAGCAGAAG 
TATTCAGATG CAGACCTGGA GCCCTTCCTA AGGAACACAT CTCAGTTCTT TCAGAGCTAT 
GTGGAGCGAG GCCTGCGCAT GATCGAGTCT GAGCGTGAGG GAAAGGGCCG CATCCAGACC 
TCTACAGTGA TCGCGCAGCA CAGCACGGAG TCATTTGTGC CGAGCTCCAG CTCTGTGCCC 
GTCAGCAGTA ATGGAGAAGA TCTAAACGCT GCCGCTTACT ACGAGAGACT GAAGATCTTA 
CGACAGCGGC GCGGGCTGGA GAACTCAGCG CCTGAAGATG AGCGTCCACC CCTCAGCTCC 
CTGAGACCGT CAGTCGCCTC CTCCACAGAC ATGCTTCACA GCAAACTATC CCAGCTGAAG 
GAGTCCCGCG AGCACTTCCA GCAGGAGCAG TCGCATTCGC ATAGCCCCAC GCGCTCCTCC 
TCACCCGCCT CCAACCTGGA CGACCTCAAG AAGAGGCTGG AGAGGATCAA GAGCAACCGC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckap5 ( XM_009297902.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296177.1 Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X3, mRNA.

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CloneID	ODa41391
Clone ID Related Accession (Same CDS sequence)	XM_009297902.2 , XM_009297902.3
Accession Version	XM_009297902.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cytoskeleton-associated protein 5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297902.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGG TTGTTGCAGG AAAGAAACCT CCTGTTAAGG CTGGCGCAAA AGATGAGGAG 
GATAAGTCTG GTCCTATTTT CATCCTCGTT CCCAATGGCA AAGAGCAGAG AATCAAAGAA 
GAGAAGTCAT TAAAGATTCT GAAGTGGAAC TTCATTACTC CTCGTGACGA GTACGTAGAG 
CAGCTCAAAA CTCAAATGTC AACATGTCTG GCCAAGTGGC TACAAGACGA GCTCTTCCAC 
TTCGACTTTC AGCATCACGT AAAGGCTATC GGAGCCATGA TTGAGCACAT GGAGGCCGAG 
TCTGAGGCTG TGATTGGCTG TCTGGACCTG GTGTTGAAGT GGTTTACTCT GCGCTTCTTT 
GACACCAACA CTAGTGTGAT CATGAAAACC CTGGAGTTCC TCAAGATGCT CTTCACCATG 
CTGAGCAGGA AAAACTACCA GCTCAATGAC TACGAGGCCT CGTCTTTCAT TCCTTACCTG 
ATCCTTAAGG TTGGAGAGTC TAAAGATGTT GTGCGCAAAG ATGTCCGTGC TATTTTGGCG 
ATGCTGTGTA AAGTCTACGC AGCGAGCAAA GTATTCCCTT ACCTTATGGA TGGGACCAAA 
TCAAAGAACT CAAAGCAAAG ATCTGAATGT CTCGAGGAGC TTGGCTGCCT GATTGAGAAC 
TTCGGTATGA ATGTATGCCA ACCGACTCCG GCCAAGGCTC TGAAAGAAAT CGCTGTCCAT 
ATTGGAGACC GTGACACTAC AGTCCGCAAT GCTGCCCTTA ATACTGTCGT AGCGGCCTAT 
AATGCCTGTG GAGACCAAGT CTTCAAACTC ATCGGCAATC TCTCCGAGAA AGAAATGAGC 
ATGCTTGAAG AGAGAATCAA ACGTTCGGCC AAGAAGACTC CAGCACCCTC AACCAAACAG 
GAGCGGCCCC AGAGGGAGCA TCCCACAAAT CCCAATGCAA CCTTTCTACG CAAGCCTGCT 
CAAGAGGAAG TGCCCAACAA GCTTAAAATA ATGTATCGCA CTTACAGGAT TCAAGCTCGG 
CAGCAGAACG CTCACTTGGA GCAGTCGGCT CCATCCATCC CGAAAGAGTT TCAGCTGGAT 
CTGGATGTCT TCGAGAACAA CCACACGTGT GCCAGCGACA TCCCTGACCT GGTGCAGCAC 
AAACTGGATG AAGTTCTGGA GCCGGTCATG ATTCCTGAGC GCAAAATTCA TTCGGTCTCT 
CCTCATTTTG ATGACATTCA CAATAGCACT GCCTCCACTA TCAACTTTGT CATCTCCCAG 
GTGGCTAGTG GAGACATTAA CACCAGTATC CAGGCTTTGG CACAGATTGA TGAGGTTTTG 
AGGCAGGCAG ACAAAGCAGA AGCCATGTCT GGACACATCG ACCAGTTCCT CATCGCCACC 
TTCATGCAGC TGCGGCTTAT TTACAACACA CACATGGCTG ACGATCGGCT GGACAAGAAG 
GAAATCTTCA AACTCTACAG TTGCATCATC GGAAACATGC TATCTCTGTT CTCGATGGAG 
AGTCTGGCAC GGGAGGCCTC GATGGGCGTC CTGAAGGACC TGATGCACGG TTTGATCACA 
CTAATGTTGG ACTCCAGAGT GGAGGACATA GAGGACGGAC AGCAGCTCAT TCGCTCTGTC 
AACCTGCTCA TGGTTCGAGT GCTAGAGAAA TCTGATCAGA CCAACATTTT GAGTGCTTTG 
CTAGTGCTGC TGCAGGACAG TCTTATCAGC ACCGCCGGCT CACCCATGTT CTCTGAACTT 
GTCATGAAGT GTCTATGGAG AATGATCCGT TTCCTACCCC AGACCATCAA CAGTATTAAT 
CTGGATCGTA TCCTGCTGGA CGTGCACAAT TTCATGAAGG TTTTCCCTAA AGAGAAGCTG 
AAGCAACTGA AGAGTGACGT TCCTCATCGG ACACTGAAGA CACTGCTGCA CACGCTCTGC 
AGACTCACAG GCGCCAAGAT ATTAGACCAC CTGTCCATGA TCGAGAACCG CAATGAGTCA 
GAGTTGGAAG CGCATCTGAG GCGAGTGGTC AAACATTCGG CAAACCTCTC AGGCCTGAAG 
TCGGACAAGG GCACGGAGAA GGGAGCACTC CGATCGGATG ACAAGATGAT CAAGGCGAAA 
GTGAGTGACA TCCTGTCTGA GATCTTCAAA AAGATCGGCT CCAAGGAAAA CACAAAAGAG 
GGTTTGACGG AGTTGTACGA GTACAAGCAG AAGTATTCAG ATGCAGACCT GGAGCCCTTC 
CTAAGGAACA CATCTCAGTT CTTTCAGAGC TATGTGGAGC GAGGCCTGCG CATGATCGAG 
TCTGAGCGTG AGGGAAAGGG CCGCATCCAG ACCTCTACAG TGATCGCGCA GCACAGCACG 
GAGTCATTTG TGCCGAGCTC CAGCTCTGTG CCCGTCAGCA GTAATGGAGA AGATCTAAAC 
GCTGCCGCTT ACTACGAGAG ACTGAAGATC TTACGACAGC GGCGCGGGCT GGAGAACTCA 
GCGCCTGAAG ATGAGCGTCC ACCCCTCAGC TCCCTGAGAC CGTCAGTCGC CTCCTCCACA 
GACATGCTTC ACAGCAAACT ATCCCAGCTG AAGGAGTCCC GCGAGCACTT CCAGCAGGAG 
CAGTCGCATT CGCATAGCCC CACGCGCTCC TCCTCACCCG CCTCCAACCT GGACGACCTC 
AAGAAGAGGC TGGAGAGGAT CAAGAGCAAC CGCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296177.1
CDS	130..6168
Translation	MGDDSEWMKLPIDQKCEHKVWKARLSGYEEALKLFQKLDEKSPEWSKYLGLIKKFVTESNAVAQLKGLEAALAFIENAHVAGKTVGEVVSGVVNKVFNQPKARAKELGTEICLMYIEIEKAEVVQDELIKGLDNKNPKIVVACIEALRKALCEFGSKIITLKPVVKVLPKLFESREKAVRDEAKLLAVEIYRWIRDALRAPLQNINSVQLKELEEEWVKVPATAPKQIRFLRSQQDLKAKFEQQQAAGGDEADGDDEEVAEAVQVDAYELLEAVEILSKLPKDFYEKIEAKKWQERKEALEAVEALTKNPKLESGDYGDLVRALKKVIGKDANVMLVSMAAKCLAGLATGLRKKFGTYAGLVVPTILEKFKEKKPQVVQALQEAIDAVFLTTTLQNISEDVLAVMDNKNPSIKQQASLFLARSFRHCTPSTLPKSVLKPFCAAFLKQVNDSAPEVRDAAFEALGTAMKVIGEKAVNPFLTDVDKLKLDKIKECADKVELIGKKGGGGGEKKERPAAKASPPVEAPAKTSGPPKKSAPAKAAGPPKKGKPAAAPSAKSKKAPETKEIIETELSPEVCEEKAAAVLPASCMQLLDSANWKERLASMEEFQKAVEQMDKSEMPCQALVKMLAKKPGWKETNFQVMQMKLSIVGLVAQKGQFSKTSALVVLDGLVDKIGDVKCGGNAKEALTAIGEACSLPWTAEQVVSIAFAQKNPKNQAETLNWLANAMKEFGFAGINVKGFINNVKTALGATNPAVRTSAIALLGVMYLYMGAPLRMFFEDEKPALLSQIDAEFVKMQGQSPPAPTRGAKKAGAEEDGDVADEDEADGGAGDIMDMLPRTDISDKITSEMVSKISDKNWKIRKEGLDEVAAVISEAKFIQANIGELPMALKGRLNDSNKLLVQQTLNILQQIAIAMGPSLKQHVKNLGIPVVTVLGDSKPNVRATAMATLNAWVEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLSEKLPTLRTVPADLMLCVPHLYTCLEDRSGDVRKKAQDALPTFMMHLGFDKMTKATGKLKPASKDQVVGMLDKARAVMPAKPAAPAKAAASKPASSAPAAKTASAPARNHSPVDDYSEPEPKPDTKKAKPAGPAAKKVVAGKKPPVKAGAKDEEDKSGPIFILVPNGKEQRIKEEKSLKILKWNFITPRDEYVEQLKTQMSTCLAKWLQDELFHFDFQHHVKAIGAMIEHMEAESEAVIGCLDLVLKWFTLRFFDTNTSVIMKTLEFLKMLFTMLSRKNYQLNDYEASSFIPYLILKVGESKDVVRKDVRAILAMLCKVYAASKVFPYLMDGTKSKNSKQRSECLEELGCLIENFGMNVCQPTPAKALKEIAVHIGDRDTTVRNAALNTVVAAYNACGDQVFKLIGNLSEKEMSMLEERIKRSAKKTPAPSTKQERPQREHPTNPNATFLRKPAQEEVPNKLKIMYRTYRIQARQQNAHLEQSAPSIPKEFQLDLDVFENNHTCASDIPDLVQHKLDEVLEPVMIPERKIHSVSPHFDDIHNSTASTINFVISQVASGDINTSIQALAQIDEVLRQADKAEAMSGHIDQFLIATFMQLRLIYNTHMADDRLDKKEIFKLYSCIIGNMLSLFSMESLAREASMGVLKDLMHGLITLMLDSRVEDIEDGQQLIRSVNLLMVRVLEKSDQTNILSALLVLLQDSLISTAGSPMFSELVMKCLWRMIRFLPQTINSINLDRILLDVHNFMKVFPKEKLKQLKSDVPHRTLKTLLHTLCRLTGAKILDHLSMIENRNESELEAHLRRVVKHSANLSGLKSDKGTEKGALRSDDKMIKAKVSDILSEIFKKIGSKENTKEGLTELYEYKQKYSDADLEPFLRNTSQFFQSYVERGLRMIESEREGKGRIQTSTVIAQHSTESFVPSSSSVPVSSNGEDLNAAAYYERLKILRQRRGLENSAPEDERPPLSSLRPSVASSTDMLHSKLSQLKESREHFQQEQSHSHSPTRSSSPASNLDDLKKRLERIKSNRQ

Target ORF information:

RefSeq Version	XM_009297902.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297902.2

ORF Insert Sequence:

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGG TTGTTGCAGG AAAGAAACCT CCTGTTAAGG CTGGCGCAAA AGATGAGGAG 
GATAAGTCTG GTCCTATTTT CATCCTCGTT CCCAATGGCA AAGAGCAGAG AATCAAAGAA 
GAGAAGTCAT TAAAGATTCT GAAGTGGAAC TTCATTACTC CTCGTGACGA GTACGTAGAG 
CAGCTCAAAA CTCAAATGTC AACATGTCTG GCCAAGTGGC TACAAGACGA GCTCTTCCAC 
TTCGACTTTC AGCATCACGT AAAGGCTATC GGAGCCATGA TTGAGCACAT GGAGGCCGAG 
TCTGAGGCTG TGATTGGCTG TCTGGACCTG GTGTTGAAGT GGTTTACTCT GCGCTTCTTT 
GACACCAACA CTAGTGTGAT CATGAAAACC CTGGAGTTCC TCAAGATGCT CTTCACCATG 
CTGAGCAGGA AAAACTACCA GCTCAATGAC TACGAGGCCT CGTCTTTCAT TCCTTACCTG 
ATCCTTAAGG TTGGAGAGTC TAAAGATGTT GTGCGCAAAG ATGTCCGTGC TATTTTGGCG 
ATGCTGTGTA AAGTCTACGC AGCGAGCAAA GTATTCCCTT ACCTTATGGA TGGGACCAAA 
TCAAAGAACT CAAAGCAAAG ATCTGAATGT CTCGAGGAGC TTGGCTGCCT GATTGAGAAC 
TTCGGTATGA ATGTATGCCA ACCGACTCCG GCCAAGGCTC TGAAAGAAAT CGCTGTCCAT 
ATTGGAGACC GTGACACTAC AGTCCGCAAT GCTGCCCTTA ATACTGTCGT AGCGGCCTAT 
AATGCCTGTG GAGACCAAGT CTTCAAACTC ATCGGCAATC TCTCCGAGAA AGAAATGAGC 
ATGCTTGAAG AGAGAATCAA ACGTTCGGCC AAGAAGACTC CAGCACCCTC AACCAAACAG 
GAGCGGCCCC AGAGGGAGCA TCCCACAAAT CCCAATGCAA CCTTTCTACG CAAGCCTGCT 
CAAGAGGAAG TGCCCAACAA GCTTAAAATA ATGTATCGCA CTTACAGGAT TCAAGCTCGG 
CAGCAGAACG CTCACTTGGA GCAGTCGGCT CCATCCATCC CGAAAGAGTT TCAGCTGGAT 
CTGGATGTCT TCGAGAACAA CCACACGTGT GCCAGCGACA TCCCTGACCT GGTGCAGCAC 
AAACTGGATG AAGTTCTGGA GCCGGTCATG ATTCCTGAGC GCAAAATTCA TTCGGTCTCT 
CCTCATTTTG ATGACATTCA CAATAGCACT GCCTCCACTA TCAACTTTGT CATCTCCCAG 
GTGGCTAGTG GAGACATTAA CACCAGTATC CAGGCTTTGG CACAGATTGA TGAGGTTTTG 
AGGCAGGCAG ACAAAGCAGA AGCCATGTCT GGACACATCG ACCAGTTCCT CATCGCCACC 
TTCATGCAGC TGCGGCTTAT TTACAACACA CACATGGCTG ACGATCGGCT GGACAAGAAG 
GAAATCTTCA AACTCTACAG TTGCATCATC GGAAACATGC TATCTCTGTT CTCGATGGAG 
AGTCTGGCAC GGGAGGCCTC GATGGGCGTC CTGAAGGACC TGATGCACGG TTTGATCACA 
CTAATGTTGG ACTCCAGAGT GGAGGACATA GAGGACGGAC AGCAGCTCAT TCGCTCTGTC 
AACCTGCTCA TGGTTCGAGT GCTAGAGAAA TCTGATCAGA CCAACATTTT GAGTGCTTTG 
CTAGTGCTGC TGCAGGACAG TCTTATCAGC ACCGCCGGCT CACCCATGTT CTCTGAACTT 
GTCATGAAGT GTCTATGGAG AATGATCCGT TTCCTACCCC AGACCATCAA CAGTATTAAT 
CTGGATCGTA TCCTGCTGGA CGTGCACAAT TTCATGAAGG TTTTCCCTAA AGAGAAGCTG 
AAGCAACTGA AGAGTGACGT TCCTCATCGG ACACTGAAGA CACTGCTGCA CACGCTCTGC 
AGACTCACAG GCGCCAAGAT ATTAGACCAC CTGTCCATGA TCGAGAACCG CAATGAGTCA 
GAGTTGGAAG CGCATCTGAG GCGAGTGGTC AAACATTCGG CAAACCTCTC AGGCCTGAAG 
TCGGACAAGG GCACGGAGAA GGGAGCACTC CGATCGGATG ACAAGATGAT CAAGGCGAAA 
GTGAGTGACA TCCTGTCTGA GATCTTCAAA AAGATCGGCT CCAAGGAAAA CACAAAAGAG 
GGTTTGACGG AGTTGTACGA GTACAAGCAG AAGTATTCAG ATGCAGACCT GGAGCCCTTC 
CTAAGGAACA CATCTCAGTT CTTTCAGAGC TATGTGGAGC GAGGCCTGCG CATGATCGAG 
TCTGAGCGTG AGGGAAAGGG CCGCATCCAG ACCTCTACAG TGATCGCGCA GCACAGCACG 
GAGTCATTTG TGCCGAGCTC CAGCTCTGTG CCCGTCAGCA GTAATGGAGA AGATCTAAAC 
GCTGCCGCTT ACTACGAGAG ACTGAAGATC TTACGACAGC GGCGCGGGCT GGAGAACTCA 
GCGCCTGAAG ATGAGCGTCC ACCCCTCAGC TCCCTGAGAC CGTCAGTCGC CTCCTCCACA 
GACATGCTTC ACAGCAAACT ATCCCAGCTG AAGGAGTCCC GCGAGCACTT CCAGCAGGAG 
CAGTCGCATT CGCATAGCCC CACGCGCTCC TCCTCACCCG CCTCCAACCT GGACGACCTC 
AAGAAGAGGC TGGAGAGGAT CAAGAGCAAC CGCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ckap5 ( XM_009297902.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296177.1 Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X3, mRNA.

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CloneID	ODa41391
Clone ID Related Accession (Same CDS sequence)	XM_009297902.2 , XM_009297902.3
Accession Version	XM_009297902.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cytoskeleton-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGG TTGTTGCAGG AAAGAAACCT CCTGTTAAGG CTGGCGCAAA AGATGAGGAG 
GATAAGTCTG GTCCTATTTT CATCCTCGTT CCCAATGGCA AAGAGCAGAG AATCAAAGAA 
GAGAAGTCAT TAAAGATTCT GAAGTGGAAC TTCATTACTC CTCGTGACGA GTACGTAGAG 
CAGCTCAAAA CTCAAATGTC AACATGTCTG GCCAAGTGGC TACAAGACGA GCTCTTCCAC 
TTCGACTTTC AGCATCACGT AAAGGCTATC GGAGCCATGA TTGAGCACAT GGAGGCCGAG 
TCTGAGGCTG TGATTGGCTG TCTGGACCTG GTGTTGAAGT GGTTTACTCT GCGCTTCTTT 
GACACCAACA CTAGTGTGAT CATGAAAACC CTGGAGTTCC TCAAGATGCT CTTCACCATG 
CTGAGCAGGA AAAACTACCA GCTCAATGAC TACGAGGCCT CGTCTTTCAT TCCTTACCTG 
ATCCTTAAGG TTGGAGAGTC TAAAGATGTT GTGCGCAAAG ATGTCCGTGC TATTTTGGCG 
ATGCTGTGTA AAGTCTACGC AGCGAGCAAA GTATTCCCTT ACCTTATGGA TGGGACCAAA 
TCAAAGAACT CAAAGCAAAG ATCTGAATGT CTCGAGGAGC TTGGCTGCCT GATTGAGAAC 
TTCGGTATGA ATGTATGCCA ACCGACTCCG GCCAAGGCTC TGAAAGAAAT CGCTGTCCAT 
ATTGGAGACC GTGACACTAC AGTCCGCAAT GCTGCCCTTA ATACTGTCGT AGCGGCCTAT 
AATGCCTGTG GAGACCAAGT CTTCAAACTC ATCGGCAATC TCTCCGAGAA AGAAATGAGC 
ATGCTTGAAG AGAGAATCAA ACGTTCGGCC AAGAAGACTC CAGCACCCTC AACCAAACAG 
GAGCGGCCCC AGAGGGAGCA TCCCACAAAT CCCAATGCAA CCTTTCTACG CAAGCCTGCT 
CAAGAGGAAG TGCCCAACAA GCTTAAAATA ATGTATCGCA CTTACAGGAT TCAAGCTCGG 
CAGCAGAACG CTCACTTGGA GCAGTCGGCT CCATCCATCC CGAAAGAGTT TCAGCTGGAT 
CTGGATGTCT TCGAGAACAA CCACACGTGT GCCAGCGACA TCCCTGACCT GGTGCAGCAC 
AAACTGGATG AAGTTCTGGA GCCGGTCATG ATTCCTGAGC GCAAAATTCA TTCGGTCTCT 
CCTCATTTTG ATGACATTCA CAATAGCACT GCCTCCACTA TCAACTTTGT CATCTCCCAG 
GTGGCTAGTG GAGACATTAA CACCAGTATC CAGGCTTTGG CACAGATTGA TGAGGTTTTG 
AGGCAGGCAG ACAAAGCAGA AGCCATGTCT GGACACATCG ACCAGTTCCT CATCGCCACC 
TTCATGCAGC TGCGGCTTAT TTACAACACA CACATGGCTG ACGATCGGCT GGACAAGAAG 
GAAATCTTCA AACTCTACAG TTGCATCATC GGAAACATGC TATCTCTGTT CTCGATGGAG 
AGTCTGGCAC GGGAGGCCTC GATGGGCGTC CTGAAGGACC TGATGCACGG TTTGATCACA 
CTAATGTTGG ACTCCAGAGT GGAGGACATA GAGGACGGAC AGCAGCTCAT TCGCTCTGTC 
AACCTGCTCA TGGTTCGAGT GCTAGAGAAA TCTGATCAGA CCAACATTTT GAGTGCTTTG 
CTAGTGCTGC TGCAGGACAG TCTTATCAGC ACCGCCGGCT CACCCATGTT CTCTGAACTT 
GTCATGAAGT GTCTATGGAG AATGATCCGT TTCCTACCCC AGACCATCAA CAGTATTAAT 
CTGGATCGTA TCCTGCTGGA CGTGCACAAT TTCATGAAGG TTTTCCCTAA AGAGAAGCTG 
AAGCAACTGA AGAGTGACGT TCCTCATCGG ACACTGAAGA CACTGCTGCA CACGCTCTGC 
AGACTCACAG GCGCCAAGAT ATTAGACCAC CTGTCCATGA TCGAGAACCG CAATGAGTCA 
GAGTTGGAAG CGCATCTGAG GCGAGTGGTC AAACATTCGG CAAACCTCTC AGGCCTGAAG 
TCGGACAAGG GCACGGAGAA GGGAGCACTC CGATCGGATG ACAAGATGAT CAAGGCGAAA 
GTGAGTGACA TCCTGTCTGA GATCTTCAAA AAGATCGGCT CCAAGGAAAA CACAAAAGAG 
GGTTTGACGG AGTTGTACGA GTACAAGCAG AAGTATTCAG ATGCAGACCT GGAGCCCTTC 
CTAAGGAACA CATCTCAGTT CTTTCAGAGC TATGTGGAGC GAGGCCTGCG CATGATCGAG 
TCTGAGCGTG AGGGAAAGGG CCGCATCCAG ACCTCTACAG TGATCGCGCA GCACAGCACG 
GAGTCATTTG TGCCGAGCTC CAGCTCTGTG CCCGTCAGCA GTAATGGAGA AGATCTAAAC 
GCTGCCGCTT ACTACGAGAG ACTGAAGATC TTACGACAGC GGCGCGGGCT GGAGAACTCA 
GCGCCTGAAG ATGAGCGTCC ACCCCTCAGC TCCCTGAGAC CGTCAGTCGC CTCCTCCACA 
GACATGCTTC ACAGCAAACT ATCCCAGCTG AAGGAGTCCC GCGAGCACTT CCAGCAGGAG 
CAGTCGCATT CGCATAGCCC CACGCGCTCC TCCTCACCCG CCTCCAACCT GGACGACCTC 
AAGAAGAGGC TGGAGAGGAT CAAGAGCAAC CGCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296177.1
CDS	162..6200
Translation	MGDDSEWMKLPIDQKCEHKVWKARLSGYEEALKLFQKLDEKSPEWSKYLGLIKKFVTESNAVAQLKGLEAALAFIENAHVAGKTVGEVVSGVVNKVFNQPKARAKELGTEICLMYIEIEKAEVVQDELIKGLDNKNPKIVVACIEALRKALCEFGSKIITLKPVVKVLPKLFESREKAVRDEAKLLAVEIYRWIRDALRAPLQNINSVQLKELEEEWVKVPATAPKQIRFLRSQQDLKAKFEQQQAAGGDEADGDDEEVAEAVQVDAYELLEAVEILSKLPKDFYEKIEAKKWQERKEALEAVEALTKNPKLESGDYGDLVRALKKVIGKDANVMLVSMAAKCLAGLATGLRKKFGTYAGLVVPTILEKFKEKKPQVVQALQEAIDAVFLTTTLQNISEDVLAVMDNKNPSIKQQASLFLARSFRHCTPSTLPKSVLKPFCAAFLKQVNDSAPEVRDAAFEALGTAMKVIGEKAVNPFLTDVDKLKLDKIKECADKVELIGKKGGGGGEKKERPAAKASPPVEAPAKTSGPPKKSAPAKAAGPPKKGKPAAAPSAKSKKAPETKEIIETELSPEVCEEKAAAVLPASCMQLLDSANWKERLASMEEFQKAVEQMDKSEMPCQALVKMLAKKPGWKETNFQVMQMKLSIVGLVAQKGQFSKTSALVVLDGLVDKIGDVKCGGNAKEALTAIGEACSLPWTAEQVVSIAFAQKNPKNQAETLNWLANAMKEFGFAGINVKGFINNVKTALGATNPAVRTSAIALLGVMYLYMGAPLRMFFEDEKPALLSQIDAEFVKMQGQSPPAPTRGAKKAGAEEDGDVADEDEADGGAGDIMDMLPRTDISDKITSEMVSKISDKNWKIRKEGLDEVAAVISEAKFIQANIGELPMALKGRLNDSNKLLVQQTLNILQQIAIAMGPSLKQHVKNLGIPVVTVLGDSKPNVRATAMATLNAWVEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLSEKLPTLRTVPADLMLCVPHLYTCLEDRSGDVRKKAQDALPTFMMHLGFDKMTKATGKLKPASKDQVVGMLDKARAVMPAKPAAPAKAAASKPASSAPAAKTASAPARNHSPVDDYSEPEPKPDTKKAKPAGPAAKKVVAGKKPPVKAGAKDEEDKSGPIFILVPNGKEQRIKEEKSLKILKWNFITPRDEYVEQLKTQMSTCLAKWLQDELFHFDFQHHVKAIGAMIEHMEAESEAVIGCLDLVLKWFTLRFFDTNTSVIMKTLEFLKMLFTMLSRKNYQLNDYEASSFIPYLILKVGESKDVVRKDVRAILAMLCKVYAASKVFPYLMDGTKSKNSKQRSECLEELGCLIENFGMNVCQPTPAKALKEIAVHIGDRDTTVRNAALNTVVAAYNACGDQVFKLIGNLSEKEMSMLEERIKRSAKKTPAPSTKQERPQREHPTNPNATFLRKPAQEEVPNKLKIMYRTYRIQARQQNAHLEQSAPSIPKEFQLDLDVFENNHTCASDIPDLVQHKLDEVLEPVMIPERKIHSVSPHFDDIHNSTASTINFVISQVASGDINTSIQALAQIDEVLRQADKAEAMSGHIDQFLIATFMQLRLIYNTHMADDRLDKKEIFKLYSCIIGNMLSLFSMESLAREASMGVLKDLMHGLITLMLDSRVEDIEDGQQLIRSVNLLMVRVLEKSDQTNILSALLVLLQDSLISTAGSPMFSELVMKCLWRMIRFLPQTINSINLDRILLDVHNFMKVFPKEKLKQLKSDVPHRTLKTLLHTLCRLTGAKILDHLSMIENRNESELEAHLRRVVKHSANLSGLKSDKGTEKGALRSDDKMIKAKVSDILSEIFKKIGSKENTKEGLTELYEYKQKYSDADLEPFLRNTSQFFQSYVERGLRMIESEREGKGRIQTSTVIAQHSTESFVPSSSSVPVSSNGEDLNAAAYYERLKILRQRRGLENSAPEDERPPLSSLRPSVASSTDMLHSKLSQLKESREHFQQEQSHSHSPTRSSSPASNLDDLKKRLERIKSNRQ

Target ORF information:

RefSeq Version	XM_009297902.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytoskeleton associated protein 5 (ckap5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297902.3

ORF Insert Sequence:

ATGGGGGATG ACAGTGAATG GATGAAGTTA CCCATCGACC AGAAATGTGA ACACAAGGTT 
TGGAAAGCCA GATTAAGTGG ATACGAGGAA GCATTGAAGC TTTTTCAAAA GCTAGACGAG 
AAGAGCCCAG AATGGAGCAA ATACCTTGGA CTTATCAAGA AATTTGTAAC AGAATCAAAT 
GCCGTAGCCC AACTCAAAGG ATTGGAGGCA GCACTTGCAT TTATTGAGAA TGCACACGTG 
GCTGGCAAGA CAGTTGGTGA AGTAGTTTCT GGAGTAGTCA ACAAAGTGTT CAACCAGCCC 
AAAGCTCGAG CCAAGGAGCT GGGGACTGAA ATCTGCCTCA TGTACATAGA GATCGAGAAA 
GCAGAAGTGG TGCAGGATGA GCTGATTAAA GGACTGGATA ACAAGAACCC CAAGATTGTC 
GTCGCTTGCA TTGAAGCACT AAGGAAAGCA CTTTGTGAAT TTGGATCAAA GATCATAACA 
CTTAAGCCTG TCGTGAAAGT GTTGCCAAAA CTATTTGAGT CTCGAGAAAA GGCAGTTCGA 
GACGAAGCCA AATTACTGGC AGTGGAGATT TATAGGTGGA TCCGCGATGC TTTGCGAGCG 
CCTCTCCAGA ATATCAACTC TGTACAGTTG AAAGAGTTGG AGGAAGAATG GGTGAAAGTG 
CCAGCTACAG CTCCTAAACA GATTAGATTC TTGCGCTCTC AGCAGGACCT GAAGGCCAAG 
TTTGAGCAGC AACAGGCTGC AGGAGGAGAT GAAGCAGACG GAGACGATGA AGAAGTGGCT 
GAAGCTGTTC AGGTTGATGC TTACGAGCTC CTGGAAGCTG TTGAGATCCT TTCAAAGCTT 
CCCAAGGATT TCTATGAAAA AATTGAAGCA AAGAAATGGC AGGAGAGGAA GGAAGCGCTG 
GAAGCAGTGG AGGCTTTAAC TAAAAACCCA AAATTAGAGA GTGGAGACTA TGGGGACCTG 
GTCCGAGCAC TAAAAAAGGT TATTGGGAAA GATGCCAATG TGATGCTAGT GTCCATGGCA 
GCCAAATGCT TGGCTGGATT GGCAACAGGA CTGAGGAAGA AGTTTGGGAC ATATGCAGGT 
CTTGTGGTGC CAACCATCTT GGAGAAGTTT AAGGAGAAGA AACCTCAAGT TGTTCAGGCC 
TTACAGGAGG CCATCGATGC AGTCTTCCTT ACTACAACCT TGCAAAACAT CAGTGAGGAT 
GTTCTGGCAG TGATGGACAA CAAGAATCCT TCCATCAAGC AACAGGCCTC ACTGTTTCTC 
GCCAGAAGTT TTCGTCACTG TACCCCAAGC ACATTGCCAA AAAGCGTTCT GAAGCCCTTC 
TGTGCTGCAT TCCTCAAGCA AGTAAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCTTTT 
GAGGCTTTGG GAACGGCCAT GAAGGTGATA GGGGAGAAAG CAGTCAACCC ATTCTTGACT 
GATGTTGACA AACTCAAGTT GGACAAGATA AAAGAGTGTG CTGATAAAGT GGAGCTCATT 
GGAAAGAAAG GAGGCGGCGG AGGAGAGAAG AAAGAGAGAC CTGCTGCAAA AGCATCTCCA 
CCTGTTGAAG CACCTGCAAA AACCTCAGGG CCTCCTAAAA AATCAGCTCC AGCTAAGGCT 
GCAGGACCTC CCAAAAAGGG CAAACCTGCT GCGGCCCCGA GTGCAAAGTC CAAGAAAGCT 
CCAGAAACTA AGGAAATAAT CGAGACCGAG TTATCTCCTG AAGTGTGTGA GGAGAAAGCA 
GCCGCTGTTC TCCCAGCCTC CTGTATGCAG CTGCTGGACA GCGCCAACTG GAAGGAGAGA 
CTTGCCAGCA TGGAGGAGTT TCAAAAGGCC GTGGAACAGA TGGACAAATC TGAAATGCCC 
TGCCAGGCTT TAGTCAAGAT GCTGGCGAAG AAACCTGGGT GGAAAGAGAC CAACTTCCAG 
GTGATGCAAA TGAAGCTCAG CATCGTGGGG CTAGTTGCAC AAAAGGGCCA GTTCTCAAAA 
ACGTCTGCAC TGGTGGTTCT GGACGGCCTG GTTGATAAGA TTGGAGATGT GAAGTGTGGA 
GGTAACGCTA AAGAGGCTCT CACTGCAATC GGAGAGGCCT GTTCTCTGCC ATGGACTGCT 
GAGCAGGTTG TCTCGATAGC CTTCGCTCAG AAGAATCCTA AAAACCAAGC AGAGACGTTG 
AACTGGTTGG CCAATGCCAT GAAGGAGTTT GGATTTGCAG GAATAAATGT CAAGGGATTC 
ATCAACAACG TCAAAACTGC TCTGGGTGCC ACAAATCCTG CTGTGAGGAC GTCTGCAATC 
GCTCTTTTGG GAGTGATGTA TCTGTATATG GGAGCTCCTC TGCGCATGTT CTTTGAAGAT 
GAAAAACCAG CCCTCCTTTC ACAAATAGAT GCTGAGTTTG TGAAGATGCA GGGTCAGTCT 
CCTCCGGCCC CCACTCGTGG AGCCAAAAAA GCAGGAGCAG AGGAGGATGG CGATGTGGCT 
GATGAAGACG AGGCAGACGG TGGAGCTGGA GACATCATGG ACATGCTGCC CAGAACTGAC 
ATCAGTGATA AAATCACATC AGAAATGGTG TCAAAGATCA GCGACAAGAA CTGGAAGATC 
AGGAAGGAGG GTCTGGATGA AGTGGCCGCT GTCATCTCTG AGGCCAAATT CATTCAGGCA 
AACATTGGCG AGCTGCCCAT GGCACTGAAG GGCCGTCTCA ATGACTCCAA CAAACTACTG 
GTCCAGCAGA CCCTCAATAT CCTGCAGCAG ATTGCCATTG CCATGGGCCC GTCTTTAAAG 
CAGCACGTCA AAAACCTGGG AATTCCTGTC GTTACTGTTC TTGGTGATAG TAAGCCTAAT 
GTTCGTGCTA CTGCCATGGC GACGCTTAAT GCATGGGTGG AGCAAACCGG AATGAAGGAG 
TGGCTTGAGG GAGAGGACCT ATCAGAGGAG CTCAAGAAGG AAAACCCTTT CCTTAGACAG 
GAGCTGCTGG GCTGGCTGTC CGAGAAGCTG CCCACCCTGC GTACGGTGCC TGCTGACCTC 
ATGCTGTGTG TGCCTCATCT GTACACCTGC CTGGAGGACC GAAGTGGAGA TGTGCGCAAG 
AAAGCCCAGG ATGCTCTTCC CACCTTCATG ATGCACCTGG GCTTTGATAA GATGACCAAG 
GCTACTGGAA AACTGAAGCC GGCCTCTAAG GACCAGGTGG TTGGCATGCT GGACAAGGCC 
AGGGCTGTGA TGCCAGCCAA GCCTGCAGCT CCTGCCAAAG CTGCAGCATC TAAACCAGCC 
TCCAGTGCCC CAGCTGCCAA AACAGCATCA GCTCCAGCCA GGAATCATAG TCCCGTTGAT 
GACTACAGTG AACCTGAACC CAAACCCGAC ACTAAGAAAG CTAAACCAGC AGGTCCTGCG 
GCTAAAAAGG TTGTTGCAGG AAAGAAACCT CCTGTTAAGG CTGGCGCAAA AGATGAGGAG 
GATAAGTCTG GTCCTATTTT CATCCTCGTT CCCAATGGCA AAGAGCAGAG AATCAAAGAA 
GAGAAGTCAT TAAAGATTCT GAAGTGGAAC TTCATTACTC CTCGTGACGA GTACGTAGAG 
CAGCTCAAAA CTCAAATGTC AACATGTCTG GCCAAGTGGC TACAAGACGA GCTCTTCCAC 
TTCGACTTTC AGCATCACGT AAAGGCTATC GGAGCCATGA TTGAGCACAT GGAGGCCGAG 
TCTGAGGCTG TGATTGGCTG TCTGGACCTG GTGTTGAAGT GGTTTACTCT GCGCTTCTTT 
GACACCAACA CTAGTGTGAT CATGAAAACC CTGGAGTTCC TCAAGATGCT CTTCACCATG 
CTGAGCAGGA AAAACTACCA GCTCAATGAC TACGAGGCCT CGTCTTTCAT TCCTTACCTG 
ATCCTTAAGG TTGGAGAGTC TAAAGATGTT GTGCGCAAAG ATGTCCGTGC TATTTTGGCG 
ATGCTGTGTA AAGTCTACGC AGCGAGCAAA GTATTCCCTT ACCTTATGGA TGGGACCAAA 
TCAAAGAACT CAAAGCAAAG ATCTGAATGT CTCGAGGAGC TTGGCTGCCT GATTGAGAAC 
TTCGGTATGA ATGTATGCCA ACCGACTCCG GCCAAGGCTC TGAAAGAAAT CGCTGTCCAT 
ATTGGAGACC GTGACACTAC AGTCCGCAAT GCTGCCCTTA ATACTGTCGT AGCGGCCTAT 
AATGCCTGTG GAGACCAAGT CTTCAAACTC ATCGGCAATC TCTCCGAGAA AGAAATGAGC 
ATGCTTGAAG AGAGAATCAA ACGTTCGGCC AAGAAGACTC CAGCACCCTC AACCAAACAG 
GAGCGGCCCC AGAGGGAGCA TCCCACAAAT CCCAATGCAA CCTTTCTACG CAAGCCTGCT 
CAAGAGGAAG TGCCCAACAA GCTTAAAATA ATGTATCGCA CTTACAGGAT TCAAGCTCGG 
CAGCAGAACG CTCACTTGGA GCAGTCGGCT CCATCCATCC CGAAAGAGTT TCAGCTGGAT 
CTGGATGTCT TCGAGAACAA CCACACGTGT GCCAGCGACA TCCCTGACCT GGTGCAGCAC 
AAACTGGATG AAGTTCTGGA GCCGGTCATG ATTCCTGAGC GCAAAATTCA TTCGGTCTCT 
CCTCATTTTG ATGACATTCA CAATAGCACT GCCTCCACTA TCAACTTTGT CATCTCCCAG 
GTGGCTAGTG GAGACATTAA CACCAGTATC CAGGCTTTGG CACAGATTGA TGAGGTTTTG 
AGGCAGGCAG ACAAAGCAGA AGCCATGTCT GGACACATCG ACCAGTTCCT CATCGCCACC 
TTCATGCAGC TGCGGCTTAT TTACAACACA CACATGGCTG ACGATCGGCT GGACAAGAAG 
GAAATCTTCA AACTCTACAG TTGCATCATC GGAAACATGC TATCTCTGTT CTCGATGGAG 
AGTCTGGCAC GGGAGGCCTC GATGGGCGTC CTGAAGGACC TGATGCACGG TTTGATCACA 
CTAATGTTGG ACTCCAGAGT GGAGGACATA GAGGACGGAC AGCAGCTCAT TCGCTCTGTC 
AACCTGCTCA TGGTTCGAGT GCTAGAGAAA TCTGATCAGA CCAACATTTT GAGTGCTTTG 
CTAGTGCTGC TGCAGGACAG TCTTATCAGC ACCGCCGGCT CACCCATGTT CTCTGAACTT 
GTCATGAAGT GTCTATGGAG AATGATCCGT TTCCTACCCC AGACCATCAA CAGTATTAAT 
CTGGATCGTA TCCTGCTGGA CGTGCACAAT TTCATGAAGG TTTTCCCTAA AGAGAAGCTG 
AAGCAACTGA AGAGTGACGT TCCTCATCGG ACACTGAAGA CACTGCTGCA CACGCTCTGC 
AGACTCACAG GCGCCAAGAT ATTAGACCAC CTGTCCATGA TCGAGAACCG CAATGAGTCA 
GAGTTGGAAG CGCATCTGAG GCGAGTGGTC AAACATTCGG CAAACCTCTC AGGCCTGAAG 
TCGGACAAGG GCACGGAGAA GGGAGCACTC CGATCGGATG ACAAGATGAT CAAGGCGAAA 
GTGAGTGACA TCCTGTCTGA GATCTTCAAA AAGATCGGCT CCAAGGAAAA CACAAAAGAG 
GGTTTGACGG AGTTGTACGA GTACAAGCAG AAGTATTCAG ATGCAGACCT GGAGCCCTTC 
CTAAGGAACA CATCTCAGTT CTTTCAGAGC TATGTGGAGC GAGGCCTGCG CATGATCGAG 
TCTGAGCGTG AGGGAAAGGG CCGCATCCAG ACCTCTACAG TGATCGCGCA GCACAGCACG 
GAGTCATTTG TGCCGAGCTC CAGCTCTGTG CCCGTCAGCA GTAATGGAGA AGATCTAAAC 
GCTGCCGCTT ACTACGAGAG ACTGAAGATC TTACGACAGC GGCGCGGGCT GGAGAACTCA 
GCGCCTGAAG ATGAGCGTCC ACCCCTCAGC TCCCTGAGAC CGTCAGTCGC CTCCTCCACA 
GACATGCTTC ACAGCAAACT ATCCCAGCTG AAGGAGTCCC GCGAGCACTT CCAGCAGGAG 
CAGTCGCATT CGCATAGCCC CACGCGCTCC TCCTCACCCG CCTCCAACCT GGACGACCTC 
AAGAAGAGGC TGGAGAGGAT CAAGAGCAAC CGCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of zebrafish magnetoreceptor and cryptochrome homologs.
Science China. Life sciences59(12)1324-1331(2016 Dec)
Zhou Z,Peng X,Chen J,Wu X,Wang Y,Hong Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Molecular evolution and functional divergence of zebrafish (Danio rerio) cryptochrome genes.
Scientific reports58113(2015 Jan)
Liu C,Hu J,Qu C,Wang L,Huang G,Niu P,Zhong Z,Hong F,Wang G,Postlethwait JH,Wang H

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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ckap5 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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