CKAP5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CKAP5
Entrez Gene ID	533980
Full Name	cytoskeleton associated protein 5
General protein information	Preferred Names cytoskeleton associated protein 5 Names cytoskeleton-associated protein 5
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010812835.2	XP_010811137.1	cytoskeleton-associated protein 5 isoform X1
XM_010812835.3	XP_010811137.1	cytoskeleton-associated protein 5 isoform X1
XM_005216463.3	XP_005216520.1	cytoskeleton-associated protein 5 isoform X2
NM_001191363.2	NP_001178292.1	cytoskeleton-associated protein 5
XM_005216463.4	XP_005216520.1	cytoskeleton-associated protein 5 isoform X2
NM_001191363.1	NP_001178292.1	cytoskeleton-associated protein 5

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011162	XP_309487.4
Canis lupus familiaris (dog)	CKAP5	XP_005631330.1
Rattus norvegicus (Norway rat)	Ckap5	XP_006234666.1
Xenopus tropicalis (tropical clawed frog)	ckap5	NP_001120559.1
Oryza sativa (rice)	Os01g0816400	NP_001044618.1
Homo sapiens (human)	CKAP5	NP_001008938.1
Pan troglodytes (chimpanzee)	CKAP5	XP_001165890.1
Arabidopsis thaliana (thale cress)	MOR1	NP_565811.2
Macaca mulatta (Rhesus monkey)	CKAP5	XP_002808118.1
Bos taurus (cattle)	CKAP5	NP_001178292.1
Mus musculus (house mouse)	Ckap5	NP_001159461.1
Gallus gallus (chicken)	CKAP5	NP_001186575.1
Danio rerio (zebrafish)	ckap5	NP_001032756.2
Drosophila melanogaster (fruit fly)	msps	NP_001163627.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CKAP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CKAP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222832	NM_001191363.2 Latest version!	Bos taurus cytoskeleton associated protein 5 (CKAP5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa40633	NM_001191363.1 Latest version!	Bos taurus cytoskeleton associated protein 5 (CKAP5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa222832	XM_010812835.3 Latest version!	Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa222833	XM_005216463.4 Latest version!	Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa26751	XM_005216463.3 Latest version!	Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa40633	XM_010812835.2 Latest version!	Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CKAP5 ( NM_001191363.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178292.1 Bos taurus cytoskeleton associated protein 5 (CKAP5), mRNA.

Price & Availability↑

CloneID	OBa222832
Clone ID Related Accession (Same CDS sequence)	XM_010812835.3 , NM_001191363.2
Accession Version	NM_001191363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527523200000
Organism	Bos taurus(cattle)
Product	cytoskeleton-associated protein 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On May 30, 2018 this sequence version replaced NM_001191363.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571258.42.1, SRR5571259.40211.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGAGACG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGCAGG ATGACCGACC TCCGTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCCACC GTGGCCTCCT CCACAGACAT GCTGCACAGC 
AAACTCTCTC AGCTCCGCGA GTCGCGGGAG CAGCACCAGC ACTCAGACCT GGACTCCAAC 
CAGACCCGCT CTTCAGGAGC CACGACCACC TCCTCCTCCA CAGCTAACAT TGACGACTTG 
AAAAAGAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178292.1
CDS	93..6191
Translation	MGDDSEWMKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGMEICLMYIEIEKGEAVQEELLKGLDNKNPKIVVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINPVQLKELEEEWVKLPTGAPRPARFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALEAVEVLVKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELVHGKKAGLAADKKDFKPVPGRAAASGAAGDKDTKDASAPKPGPLKKAPAAKPGGPPKKGKPAAQGGTGGTGTKSKKAVETKEMVEPELSIEVCEEKASAVLPATCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQIVLDGLVDKIGDVKCGNNAKEAMTAIAEACVLPWTAEQVMSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTSAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQNPPAPTRGISKHSTSGTDEGEDGDEPDDGGSDAVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAAIINEAKFIQPNIGELPTALKGRLNDSNKILVQQTLTILQQLAVAMGPNIKQHVKNLGIPVITVLGDSKNNVRSAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPQLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKANMPAKPTAPAKATSRPVGGSAPAKFQPASAPAEDSVSGTLDSKPDPKKAKALGVSSKTKTAQGKKVPSKTSLKEDDDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWVTLRFFDTNTSVLMKTLEYLKLLFTLLSDEEYHLTENEASSFIPYLILKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAIHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGTLSEKDMSMLEERIKRSAKRPSAAPVKQVEEKPQRTQSISSNTNMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDSGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVVRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRLPTSAGISPQMEVTCVPTATSTASSLGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTRSSGATTTSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	NM_001191363.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cytoskeleton associated protein 5 (CKAP5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191363.2

ORF Insert Sequence:

ATGGGAGACG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGCAGG ATGACCGACC TCCGTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCCACC GTGGCCTCCT CCACAGACAT GCTGCACAGC 
AAACTCTCTC AGCTCCGCGA GTCGCGGGAG CAGCACCAGC ACTCAGACCT GGACTCCAAC 
CAGACCCGCT CTTCAGGAGC CACGACCACC TCCTCCTCCA CAGCTAACAT TGACGACTTG 
AAAAAGAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKAP5 ( NM_001191363.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178292.1 Bos taurus cytoskeleton associated protein 5 (CKAP5), mRNA.

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CloneID	OBa40633
Clone ID Related Accession (Same CDS sequence)	XM_010812835.2 , NM_001191363.1
Accession Version	NM_001191363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Bos taurus(cattle)
Product	cytoskeleton-associated protein 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02041587.1, DAAA02041586.1 and DAAA02041585.1. On Jul 17, 2010 this sequence version replaced XM_001790245.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGGGAGATG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGCAGG ATGACCGACC TCCGTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCCACC GTGGCCTCCT CCACAGACAT GCTGCACAGC 
AAACTCTCTC AGCTCCGCGA GTCGCGGGAG CAGCACCAGC ACTCAGACCT GGACTCCAAC 
CAGACCCGCT CTTCAGGAGC CACGACCACC TCCTCCTCCA CAGCTAACAT TGACGACTTG 
AAAAAGAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178292.1
CDS	93..6191
Translation	MGDDSEWMKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGMEICLMYIEIEKGEAVQEELLKGLDNKNPKIVVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINPVQLKELEEEWVKLPTGAPRPARFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALEAVEVLVKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELVHGKKAGLAADKKDFKPVPGRAAASGAAGDKDTKDASAPKPGPLKKAPAAKPGGPPKKGKPAAQGGTGGTGTKSKKAVETKEMVEPELSIEVCEEKASAVLPATCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQIVLDGLVDKIGDVKCGNNAKEAMTAIAEACVLPWTAEQVMSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTSAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQNPPAPTRGISKHSTSGTDEGEDGDEPDDGGSDAVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAAIINEAKFIQPNIGELPTALKGRLNDSNKILVQQTLTILQQLAVAMGPNIKQHVKNLGIPVITVLGDSKNNVRSAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPQLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKANMPAKPTAPAKATSRPVGGSAPAKFQPASAPAEDSVSGTLDSKPDPKKAKALGVSSKTKTAQGKKVPSKTSLKEDDDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWVTLRFFDTNTSVLMKTLEYLKLLFTLLSDEEYHLTENEASSFIPYLILKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAIHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGTLSEKDMSMLEERIKRSAKRPSAAPVKQVEEKPQRTQSISSNTNMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDSGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVVRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRLPTSAGISPQMEVTCVPTATSTASSLGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTRSSGATTTSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	NM_001191363.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cytoskeleton associated protein 5 (CKAP5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191363.1

ORF Insert Sequence:

ATGGGAGATG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGCAGG ATGACCGACC TCCGTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCCACC GTGGCCTCCT CCACAGACAT GCTGCACAGC 
AAACTCTCTC AGCTCCGCGA GTCGCGGGAG CAGCACCAGC ACTCAGACCT GGACTCCAAC 
CAGACCCGCT CTTCAGGAGC CACGACCACC TCCTCCTCCA CAGCTAACAT TGACGACTTG 
AAAAAGAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKAP5 ( XM_010812835.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811137.1 Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X1, mRNA.

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CloneID	OBa222832
Clone ID Related Accession (Same CDS sequence)	XM_010812835.3 , NM_001191363.2
Accession Version	XM_010812835.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	cytoskeleton-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGCAGG ATGACCGACC TCCGTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCCACC GTGGCCTCCT CCACAGACAT GCTGCACAGC 
AAACTCTCTC AGCTCCGCGA GTCGCGGGAG CAGCACCAGC ACTCAGACCT GGACTCCAAC 
CAGACCCGCT CTTCAGGAGC CACGACCACC TCCTCCTCCA CAGCTAACAT TGACGACTTG 
AAAAAGAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811137.1
CDS	144..6242
Translation	MGDDSEWMKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGMEICLMYIEIEKGEAVQEELLKGLDNKNPKIVVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINPVQLKELEEEWVKLPTGAPRPARFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALEAVEVLVKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELVHGKKAGLAADKKDFKPVPGRAAASGAAGDKDTKDASAPKPGPLKKAPAAKPGGPPKKGKPAAQGGTGGTGTKSKKAVETKEMVEPELSIEVCEEKASAVLPATCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQIVLDGLVDKIGDVKCGNNAKEAMTAIAEACVLPWTAEQVMSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTSAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQNPPAPTRGISKHSTSGTDEGEDGDEPDDGGSDAVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAAIINEAKFIQPNIGELPTALKGRLNDSNKILVQQTLTILQQLAVAMGPNIKQHVKNLGIPVITVLGDSKNNVRSAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPQLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKANMPAKPTAPAKATSRPVGGSAPAKFQPASAPAEDSVSGTLDSKPDPKKAKALGVSSKTKTAQGKKVPSKTSLKEDDDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWVTLRFFDTNTSVLMKTLEYLKLLFTLLSDEEYHLTENEASSFIPYLILKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAIHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGTLSEKDMSMLEERIKRSAKRPSAAPVKQVEEKPQRTQSISSNTNMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDSGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVVRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRLPTSAGISPQMEVTCVPTATSTASSLGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTRSSGATTTSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	XM_010812835.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812835.3

ORF Insert Sequence:

ATGGGAGACG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGCAGG ATGACCGACC TCCGTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCCACC GTGGCCTCCT CCACAGACAT GCTGCACAGC 
AAACTCTCTC AGCTCCGCGA GTCGCGGGAG CAGCACCAGC ACTCAGACCT GGACTCCAAC 
CAGACCCGCT CTTCAGGAGC CACGACCACC TCCTCCTCCA CAGCTAACAT TGACGACTTG 
AAAAAGAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKAP5 ( XM_005216463.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216520.1 Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X2, mRNA.

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CloneID	OBa222833
Clone ID Related Accession (Same CDS sequence)	XM_005216463.4
Accession Version	XM_005216463.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	cytoskeleton-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216463.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGGATG ACCGACCTCC GTTGACCTCT 
TTGCTCTCCA AACCAGCAGT TCCCACCGTG GCCTCCTCCA CAGACATGCT GCACAGCAAA 
CTCTCTCAGC TCCGCGAGTC GCGGGAGCAG CACCAGCACT CAGACCTGGA CTCCAACCAG 
ACCCGCTCTT CAGGAGCCAC GACCACCTCC TCCTCCACAG CTAACATTGA CGACTTGAAA 
AAGAGACTGG AGAGAATAAA GAGCAGTCGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216520.1
CDS	135..6230
Translation	MGDDSEWMKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGMEICLMYIEIEKGEAVQEELLKGLDNKNPKIVVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINPVQLKELEEEWVKLPTGAPRPARFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALEAVEVLVKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELVHGKKAGLAADKKDFKPVPGRAAASGAAGDKDTKDASAPKPGPLKKAPAAKPGGPPKKGKPAAQGGTGGTGTKSKKAVETKEMVEPELSIEVCEEKASAVLPATCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQIVLDGLVDKIGDVKCGNNAKEAMTAIAEACVLPWTAEQVMSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTSAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQNPPAPTRGISKHSTSGTDEGEDGDEPDDGGSDAVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAAIINEAKFIQPNIGELPTALKGRLNDSNKILVQQTLTILQQLAVAMGPNIKQHVKNLGIPVITVLGDSKNNVRSAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPQLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKANMPAKPTAPAKATSRPVGGSAPAKFQPASAPAEDSVSGTLDSKPDPKKAKALGVSSKTKTAQGKKVPSKTSLKEDDDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWVTLRFFDTNTSVLMKTLEYLKLLFTLLSDEEYHLTENEASSFIPYLILKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAIHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGTLSEKDMSMLEERIKRSAKRPSAAPVKQVEEKPQRTQSISSNTNMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDSGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVVRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRLPTSAGISPQMEVTCVPTATSTASSLGNTNGEEVGPSVYLERLKILRQRCGLDNTKDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTRSSGATTTSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	XM_005216463.4
Organism	Bos taurus(cattle)
Definition	Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216463.4

ORF Insert Sequence:

ATGGGAGACG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGGATG ACCGACCTCC GTTGACCTCT 
TTGCTCTCCA AACCAGCAGT TCCCACCGTG GCCTCCTCCA CAGACATGCT GCACAGCAAA 
CTCTCTCAGC TCCGCGAGTC GCGGGAGCAG CACCAGCACT CAGACCTGGA CTCCAACCAG 
ACCCGCTCTT CAGGAGCCAC GACCACCTCC TCCTCCACAG CTAACATTGA CGACTTGAAA 
AAGAGACTGG AGAGAATAAA GAGCAGTCGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKAP5 ( XM_005216463.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216520.1 Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X2, mRNA.

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CloneID	OBa26751
Clone ID Related Accession (Same CDS sequence)	XM_005216463.3
Accession Version	XM_005216463.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	cytoskeleton-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216463.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGGATG ACCGACCTCC GTTGACCTCT 
TTGCTCTCCA AACCAGCAGT TCCCACCGTG GCCTCCTCCA CAGACATGCT GCACAGCAAA 
CTCTCTCAGC TCCGCGAGTC GCGGGAGCAG CACCAGCACT CAGACCTGGA CTCCAACCAG 
ACCCGCTCTT CAGGAGCCAC GACCACCTCC TCCTCCACAG CTAACATTGA CGACTTGAAA 
AAGAGACTGG AGAGAATAAA GAGCAGTCGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216520.1
CDS	117..6212
Translation	MGDDSEWMKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGMEICLMYIEIEKGEAVQEELLKGLDNKNPKIVVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINPVQLKELEEEWVKLPTGAPRPARFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALEAVEVLVKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELVHGKKAGLAADKKDFKPVPGRAAASGAAGDKDTKDASAPKPGPLKKAPAAKPGGPPKKGKPAAQGGTGGTGTKSKKAVETKEMVEPELSIEVCEEKASAVLPATCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQIVLDGLVDKIGDVKCGNNAKEAMTAIAEACVLPWTAEQVMSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTSAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQNPPAPTRGISKHSTSGTDEGEDGDEPDDGGSDAVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAAIINEAKFIQPNIGELPTALKGRLNDSNKILVQQTLTILQQLAVAMGPNIKQHVKNLGIPVITVLGDSKNNVRSAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPQLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKANMPAKPTAPAKATSRPVGGSAPAKFQPASAPAEDSVSGTLDSKPDPKKAKALGVSSKTKTAQGKKVPSKTSLKEDDDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWVTLRFFDTNTSVLMKTLEYLKLLFTLLSDEEYHLTENEASSFIPYLILKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAIHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGTLSEKDMSMLEERIKRSAKRPSAAPVKQVEEKPQRTQSISSNTNMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDSGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVVRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRLPTSAGISPQMEVTCVPTATSTASSLGNTNGEEVGPSVYLERLKILRQRCGLDNTKDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTRSSGATTTSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	XM_005216463.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216463.3

ORF Insert Sequence:

ATGGGAGATG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGGATG ACCGACCTCC GTTGACCTCT 
TTGCTCTCCA AACCAGCAGT TCCCACCGTG GCCTCCTCCA CAGACATGCT GCACAGCAAA 
CTCTCTCAGC TCCGCGAGTC GCGGGAGCAG CACCAGCACT CAGACCTGGA CTCCAACCAG 
ACCCGCTCTT CAGGAGCCAC GACCACCTCC TCCTCCACAG CTAACATTGA CGACTTGAAA 
AAGAGACTGG AGAGAATAAA GAGCAGTCGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKAP5 ( XM_010812835.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811137.1 Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X1, mRNA.

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CloneID	OBa40633
Clone ID Related Accession (Same CDS sequence)	XM_010812835.2 , NM_001191363.1
Accession Version	XM_010812835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	cytoskeleton-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGCAGG ATGACCGACC TCCGTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCCACC GTGGCCTCCT CCACAGACAT GCTGCACAGC 
AAACTCTCTC AGCTCCGCGA GTCGCGGGAG CAGCACCAGC ACTCAGACCT GGACTCCAAC 
CAGACCCGCT CTTCAGGAGC CACGACCACC TCCTCCTCCA CAGCTAACAT TGACGACTTG 
AAAAAGAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811137.1
CDS	126..6224
Translation	MGDDSEWMKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGMEICLMYIEIEKGEAVQEELLKGLDNKNPKIVVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINPVQLKELEEEWVKLPTGAPRPARFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALEAVEVLVKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELVHGKKAGLAADKKDFKPVPGRAAASGAAGDKDTKDASAPKPGPLKKAPAAKPGGPPKKGKPAAQGGTGGTGTKSKKAVETKEMVEPELSIEVCEEKASAVLPATCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQIVLDGLVDKIGDVKCGNNAKEAMTAIAEACVLPWTAEQVMSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTSAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQNPPAPTRGISKHSTSGTDEGEDGDEPDDGGSDAVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAAIINEAKFIQPNIGELPTALKGRLNDSNKILVQQTLTILQQLAVAMGPNIKQHVKNLGIPVITVLGDSKNNVRSAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPQLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKANMPAKPTAPAKATSRPVGGSAPAKFQPASAPAEDSVSGTLDSKPDPKKAKALGVSSKTKTAQGKKVPSKTSLKEDDDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWVTLRFFDTNTSVLMKTLEYLKLLFTLLSDEEYHLTENEASSFIPYLILKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAIHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGTLSEKDMSMLEERIKRSAKRPSAAPVKQVEEKPQRTQSISSNTNMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDSGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVVRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRLPTSAGISPQMEVTCVPTATSTASSLGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTRSSGATTTSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	XM_010812835.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cytoskeleton associated protein 5 (CKAP5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812835.2

ORF Insert Sequence:

ATGGGAGATG ACAGTGAGTG GATGAAACTG CCGGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GATTAAGTGG GTATGAAGAA GCCCTGAAGA TCTTCCAGAA AATAAAAGAT 
GAAAAGAGCC CAGAATGGTC CAAATTTTTA GGATTGATCA AAAAGTTTGT GACGGATTCC 
AATGCAGTGG TTCAGTTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACGGG AGAGGTTGTG TCAGGTGTTG TAAGTAAAGT ATTCAACCAA 
CCCAAAGCCA AAGCCAAGGA GCTAGGCATG GAGATTTGTC TAATGTACAT AGAGATTGAG 
AAAGGAGAAG CTGTACAAGA GGAGCTGCTG AAAGGCCTGG ACAATAAGAA TCCCAAGATC 
GTTGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTCGGTTC CAAAATCATC 
TTACTGAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AATCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAATTAAT TGCTGTAGAG ATTTATAGAT GGATTCGAGA TGCTCTGAGA 
CCCCCGTTAC AAAACATAAA CCCTGTCCAG TTGAAAGAAC TAGAAGAAGA GTGGGTCAAG 
CTGCCAACAG GTGCTCCTAG ACCAGCTAGG TTTCTGCGTT CCCAGCAAGA ACTAGAAGCT 
AAATTGGAAC AGCAACAGTC TGCTGGTGGA GATGCTGAGG GAGGTGGTGA CGATGGTGAT 
GAGGTACCAC AGATAGATGC TTATGAGCTT TTGGAAGCAG TAGAAATTCT TTCCAAGCTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAAAGAAA AGAAGCCCTG 
GAAGCTGTGG AAGTACTAGT GAAAAACCCC AAACTGGAAG CAGGCGATTA TGCAGATTTA 
GTAAAAGCTT TGAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT AGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGAAAGA AATTTGGACA ATATGCAGGA 
CACGTTGTAC CAACCATTTT GGAGAAATTC AAAGAGAAGA AGCCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AACAGACATC TCTATTTATT 
GCAAGAAGCT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTACT AAAGCCCTTT 
TGTGCTGCTC TACTGAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCTGCATTT 
GAAGCTTTAG GTACTGCTTT GAAGGTGGTT GGTGAGAAAG CAGTAAACCC ATTCTTAGCT 
GATGTAGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAAGT AGAGCTGGTA 
CATGGTAAGA AAGCTGGACT TGCAGCTGAC AAGAAGGATT TCAAGCCTGT GCCCGGAAGG 
GCGGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACGCCTCAGC ACCCAAACCA 
GGACCTTTAA AAAAGGCGCC TGCTGCGAAG CCTGGCGGGC CACCAAAGAA GGGAAAACCG 
GCTGCTCAAG GAGGCACAGG AGGCACTGGG ACCAAGAGCA AGAAAGCAGT AGAGACTAAA 
GAAATGGTGG AGCCTGAGCT CTCGATCGAA GTATGTGAAG AAAAAGCTTC AGCTGTCCTT 
CCTGCTACCT GTATACAGCT TCTCGACAGC AGTAACTGGA AGGAAAGGCT GGCCTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAACTAATG GACCGAACTG AAATGCCTTG CCAGGCATTA 
GTGAGGATGC TGGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCACA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC TTCAGCACAG 
ATCGTCTTGG ATGGACTTGT AGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCTAAA 
GAAGCTATGA CAGCAATAGC TGAAGCGTGT GTGTTGCCGT GGACAGCTGA ACAGGTTATG 
TCAATGGCGT TCTCACAGAA GAATCCCAAG AATCAATCAG AAACTCTGAA CTGGCTATCA 
AATGCAATAA AAGAATTTGG TTTTTCTGGG CTGAATGTGA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACGT CTGCCATCAC TCTGCTTGGT 
GTGATGTATC TGTACGTTGG TCCTTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAGAACCC ACCGGCTCCA 
ACCAGAGGGA TTTCTAAACA CAGCACAAGT GGGACAGACG AAGGGGAAGA TGGAGATGAA 
CCAGATGACG GGGGCAGTGA TGCTGTGGAT CTTTTGCCAA GGACAGAAAT CAGTGATAAA 
ATCACTTCAG AGTTGGTGTC TAAGATTGGT GACAAGAATT GGAAGATCAG GAAAGAAGGC 
CTTGATGAAG TAGCAGCTAT TATCAATGAA GCAAAATTCA TCCAGCCAAA CATAGGTGAA 
CTTCCAACAG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT GCAGCAGACG 
TTGACTATCC TCCAGCAGCT GGCAGTAGCC ATGGGCCCCA ACATCAAGCA GCATGTGAAG 
AACTTAGGAA TCCCTGTCAT CACAGTGCTT GGAGACAGCA AGAATAATGT TCGATCTGCT 
GCCCTAGCAA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAGGGG 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC GACTCTCCGT TCCACCCCCA CAGACCTTAT CCTTTGTGTT 
CCCCAGCTCT ATTCCTGCCT TGAGGACCGC AATGGAGACG TGCGAAAGAA GGCCCAGGAC 
GCCTTGCCGT TCTTCATGAT GCACTTAGGA TATGAGAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAACCAA CTTCTAAAGA TCAGGTGTTG GCCATGTTAG AGAAGGCCAA AGCTAACATG 
CCAGCCAAGC CCACTGCACC TGCTAAAGCA ACATCCAGAC CAGTGGGAGG GTCAGCTCCA 
GCCAAATTCC AGCCTGCCTC AGCACCTGCT GAAGATTCTG TTTCTGGCAC TCTAGACTCC 
AAGCCTGATC CGAAAAAGGC CAAGGCTCTA GGAGTTTCCT CTAAAACAAA GACTGCACAA 
GGAAAGAAAG TTCCGAGCAA AACCAGCTTA AAGGAGGATG ACGACAAATC CGGTCCTATT 
TTTATTGTTG TTCCAAATGG AAAGGAGCAA AGGATGAAAG ATGAGAAAGG ACTAAAGGTA 
CTAAAGTGGA ATTTTACTAC TCCACGGGAT GAATACATCG AGCAGTTAAA AACTCAGATG 
TCTAGCTGTG TAGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAGGCCC TTGCTGTCAT GGTTGATCAC TTGGAGAGCG AAAAAGAGGG TGTCATTGGT 
TGCCTGGATC TTATCTTAAA GTGGGTTACG TTGCGGTTTT TTGACACCAA TACAAGCGTC 
CTCATGAAAA CCCTAGAATA TTTAAAATTG CTCTTCACCC TGCTAAGTGA TGAGGAATAT 
CATCTTACTG AGAACGAAGC ATCTTCCTTC ATCCCCTATC TCATCCTCAA GGTCGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTCCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCCAGCA AGATGTTCCC TTTCATCATG GAAGGAACCA AATCCAAAAA CTCCAAGCAG 
AGAGCAGAGT GCCTGGAAGA ACTGGGGTGT CTGGTCGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAG ATAGCGATTC ACATAGGAGA CCGTGACAAT 
GCTGTGCGCA ATGCTGCGCT CAACACCATC GTCACGGTGT ACAATGTACA CGGGGATCAA 
GTGTTCAAGT TGATTGGAAC CCTTTCTGAA AAAGATATGA GCATGCTTGA GGAGAGGATT 
AAGCGGTCAG CAAAAAGGCC TTCGGCTGCA CCAGTAAAAC AGGTGGAAGA GAAACCTCAG 
CGCACACAGA GCATAAGCTC CAACACCAAC ATGCTGCGCA AGGGACCAGC TGAGGACATG 
TCCTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTT 
CGCCGAGAAT TCCAGCTGGA CCTAGATGAG ATTGAGAATG ACAGTGGTAC AGTGCGGTGT 
GAAATGCCAG AGCTTGTTCA GCACAAACTG GATGACATTT TTGAACCAGT CCTTATTCCT 
GAGCCCAAGA TCCGGGCTGT TTCTCCACAC TTTGATGATA TGCACAGTAA TACAGCATCC 
ACAATCAATT TTATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG CATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCTGGCCAT 
ATTGATCAGT TCCTCATAGC TACGTTCATG CAGCTGAGAC TCATCTACAA CACGCACATG 
GCAGATGAGA AGCTGGAGAA GGATGAAATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAAAGCCTC GCCCGGGAGG CCTCCACTGG AGTTTTGAAA 
GACCTGATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GAATTGAAGA TCTGGAGGAA 
GGGCAGCAGG TGGTCCGCTC TGTGAACCTC TTGGTGGTGA AAGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTTCTTGTT TTGCTCCAAG ACAGTCTCCT AGCAACAGCC 
AGTTCTCCCA AGTTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGGATGGT TCGACTACTG 
CCTGACACCA TCAACAGCAT TAATCTGGAC AGAATTCTTC TGGATATACA CATTTTCATG 
AAGGTCTTCC CCAAAGAAAA ACTCAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAAACGCTGC TGCACACTCT ATGCAAACTG AAAGGGCCCA AGATCCTGGA CCACCTGACT 
ATGATTGACA ACAAGAACGA GTCAGAGTTG GAGGCCCACC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACCGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATT 
GATGAAAAAT CATCAAAGGC CAAAGTGAAC GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AGAATACTAA AGAGGGCCTA GCAGAGTTAT ATGAATACAA GAAGAAATAT 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AACTCCTCAC AGTTCTTCCA GAGCTATGTT 
GAGAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGCCGCCT TCCCACCTCG 
GCAGGCATCT CCCCTCAGAT GGAGGTCACG TGCGTGCCCA CCGCCACAAG CACAGCGTCC 
TCCTTAGGCA ACACAAACGG AGAAGAGGTG GGGCCGTCCG TCTACCTGGA GAGGCTGAAA 
ATCCTCCGGC AGCGATGCGG TCTGGACAAT ACAAAGCAGG ATGACCGACC TCCGTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCCACC GTGGCCTCCT CCACAGACAT GCTGCACAGC 
AAACTCTCTC AGCTCCGCGA GTCGCGGGAG CAGCACCAGC ACTCAGACCT GGACTCCAAC 
CAGACCCGCT CTTCAGGAGC CACGACCACC TCCTCCTCCA CAGCTAACAT TGACGACTTG 
AAAAAGAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CKAP5 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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