capn2l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	capn2l
Entrez Gene ID	445091
Full Name	zgc:92480
General protein information	Preferred Names zgc:92480 Names uncharacterized protein LOC445091
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003485.1	NP_001003485.1	uncharacterized protein LOC445091
NM_001003485.2	NP_001003485.1	calpain-2 catalytic subunit

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CAPN2	XP_001098172.1
Mus musculus (house mouse)	Capn2	NP_033924.2
Danio rerio (zebrafish)	zgc:92480	NP_001003485.1
Rattus norvegicus (Norway rat)	Capn2	NP_058812.1
Xenopus tropicalis (tropical clawed frog)	capn2.2	NP_001005446.1
Homo sapiens (human)	CAPN2	NP_001739.2
Pan troglodytes (chimpanzee)	CAPN2	XP_003308813.1
Danio rerio (zebrafish)	capn2l	NP_001017807.1
Canis lupus familiaris (dog)	CAPN2	XP_005622713.1
Bos taurus (cattle)	CAPN2	NP_001096556.1
Gallus gallus (chicken)	CAPN2	NP_990411.1

Gene Ontology
Bibliography

Related articles in PubMed

Vascular defects of
Cho HJ, Lee JG, Kim JH, Kim SY, Huh YH, Kim HJ, Lee KS, Yu K, Lee JS
Disease models & mechanisms12(5)(2019 05)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
Tao T, Shi H, Guan Y, Huang D, Chen Y, Lane DP, Chen J, Peng J
Cell research23(5)620-34(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following capn2l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the capn2l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31394	NM_001003485.2 Latest version!	Danio rerio calpain 2, (m/II) large subunit, like (capn2l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa31394	NM_001003485.1	Danio rerio zgc:92480 (zgc:92480), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

capn2l ( NM_001003485.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003485.1 Danio rerio zgc:92480 (zgc:92480), mRNA.

Price & Availability↑

CloneID	ODa31394
Clone ID Related Accession (Same CDS sequence)	NM_001003485.1 , NM_001003485.2
Accession Version	NM_001003485.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1501862400000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC445091
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC076557.1. On Jul 6, 2005 this sequence version replaced XM_683499.1. ##Evidence-Data-START## Transcript exon combination :: BC076557.1, BC154289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGGAA TCGCGATTAA ACTCCAACAC AAGCGGGCAC AAGCGGCGGG CATCGGTACC 
AACGCTCAAG CGGTCAAATT CCTTAACCAG GACTATGAAG CCCTCAAACG AGAGTGTCTG 
GAGTCCGGAC GTCTGTTTCA CGATGACATG TTTGAAGCTA ATGTGTCTGC CCTCGGGTTT 
AAGGAACTAG GACCAAACTC GTCTAAAGTG CGCGGGGTTG AGTGGCTGAG ACCCAAACAA 
CTGACGTCCA ACCCAACATT CATTAGTGGA GGAGCGACCA GAACAGACAT CTGCCAGGGC 
GCTTTGGGTG ACTGTTGGCT ATTAGCAGCC ATCGCTTCTC TTACCCTGAA CCAGGACGTG 
TTTGCTCGTG TTGTGCCAGC TGGTCAGAGT TTTGATGGCG ACTATGCTGG AATATTTCAT 
TTTCAGTTTT GGCAGTTTGG TGAATGGGTG GATGTAGTCA TCGATGACCG GCTGCCGGCT 
AGAAACGGAG AGCTGCTGTT TGTCCACTCG GCCGAGGGCT CTGAGTTCTG GAGTGCTCTT 
TTGGAGAAAG CCTATGCAAA GTTGAACGGC TGCTATGAAG CTCTTTCTGG AGGCACCACC 
ACTGAAGGTT TTGAGGATTT CACTGGAGGC ATTGCCGAGG TCCACGAACT GGCCAAAGCA 
GGTCCAAACC TCTTCAAAAC CATCCAGAAG GCTCTTAGCT GGGGCTCGTT GCTGGGCTGC 
TCCATAGATA TCACAAGCTC TTCTGACTCA GAGGCCATTA CTAGTCAGAA ACTGGTGAAA 
GGACACGCCT ACTCGGTTAC AGGAGCTGAA GAGGTGGAGT ACAGGGGTGA CTTAACTAAA 
CTGATTCGAA TCAGGAACCC CTGGGGGCAG GTCGAGTGGA CGGGACCCTG GAGTGATGGG 
TCATCAGAAT GGCGTCAGAT CAGTGACAAC GACAGAGAAA GACTGAGCTC CAAAGCTGAA 
GATGGAGAAT TCTGGATGTC TTTCTCTGAC TTCATGCGTC ATTACTCACG GGTGGAGATC 
TGCAATCTGA CGCCCGATGC GCTCACAGAT GAAAGTGTTA ATAAATGGGC TCTGTCCAAG 
TTTGATGGCA ACTGGAGGAA TGGATCAACT GCTGGAGGCT GCAGGAATTA CCCCAACTCG 
TTCTGGATGA ATCCTCAGTT TCTGATTAAA CTGGAGGAGC AGGATGACGA CCCGACTGAT 
AATGAAGCAG GATGCAGCTT TGTTGTGGGA CTGATCCAGA AGAACCGCCG CAAAATGAGG 
AAGGTCGGAG AGGACATGAA CACCATCGGC TTTGCAATCT ATGAGGTTCC TGACCAGTTT 
GTGGGACAGC GGAACGTCCA TCTGGATCGT AACTTTTTCG TGCGACATGC ATCAGCTGCT 
CGGTCCGAGA CCTTCATCAA CCTGCGGGAG GTTTGCTCTC GCTTCTGTCT GCCCCCCGGC 
GAGTACCTCA TCGTGCCCTC TACTTTTGAG CCCAACAAAG ATGGTGACTT CTGTGTCCGC 
GTTTTCTCAG AAAAACAAGC AGAGTTCCAA GAGCTTGATG ATCCTGTCGA GTCCAAGGTT 
GCGGAGATTG AAATAACAGA GGGTGATATT GATAGCCGCT TCAAGAAACT GTTTGGACAG 
CTTGCTGGAG CTGACTGTGA GATCTCTGCA TTTGAGCTGC AAAAAATCCT GAACAATGTG 
ATAGCTAAAC GCAAAGAGAT TAAAACTGAT GGATTTAGTT TGGAGACTTG CCGCAACATG 
GTGAACCTAC TGGATAAAGA CGGCACTGGA AAACTAGGCC TGCTTGAATT TAAGATTCTG 
TGGACTAAAA TTGAGCTGTT CGTGGACGTC TACAGTAAAA ATGACAAAGA CCAGTCTGGC 
ACAATGAGCT CTATGGAGAT GAGAGAAGCC GTAGAGAAAG CAGGGTTTTC TCTGAATAAT 
GCTCTACACC AGATCCTTGT TGCACGATAC AGCGAATCCA ACCTCACCAT CGACTTTGAC 
AACTTTGTGG CCTGTCTTAT ACGCCTCGAA TGCATGTTCA AGGCATTTAA GGTGCTGGAC 
AAGGACAAGA ATGGCACCGT CGAGCTGAAC ATGATTGAGT GGCTGAATGT TTCCATGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003485.1
CDS	16..2118
Translation	MSGIAIKLQHKRAQAAGIGTNAQAVKFLNQDYEALKRECLESGRLFHDDMFEANVSALGFKELGPNSSKVRGVEWLRPKQLTSNPTFISGGATRTDICQGALGDCWLLAAIASLTLNQDVFARVVPAGQSFDGDYAGIFHFQFWQFGEWVDVVIDDRLPARNGELLFVHSAEGSEFWSALLEKAYAKLNGCYEALSGGTTTEGFEDFTGGIAEVHELAKAGPNLFKTIQKALSWGSLLGCSIDITSSSDSEAITSQKLVKGHAYSVTGAEEVEYRGDLTKLIRIRNPWGQVEWTGPWSDGSSEWRQISDNDRERLSSKAEDGEFWMSFSDFMRHYSRVEICNLTPDALTDESVNKWALSKFDGNWRNGSTAGGCRNYPNSFWMNPQFLIKLEEQDDDPTDNEAGCSFVVGLIQKNRRKMRKVGEDMNTIGFAIYEVPDQFVGQRNVHLDRNFFVRHASAARSETFINLREVCSRFCLPPGEYLIVPSTFEPNKDGDFCVRVFSEKQAEFQELDDPVESKVAEIEITEGDIDSRFKKLFGQLAGADCEISAFELQKILNNVIAKRKEIKTDGFSLETCRNMVNLLDKDGTGKLGLLEFKILWTKIELFVDVYSKNDKDQSGTMSSMEMREAVEKAGFSLNNALHQILVARYSESNLTIDFDNFVACLIRLECMFKAFKVLDKDKNGTVELNMIEWLNVSML

Target ORF information:

RefSeq Version	NM_001003485.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:92480 (zgc:92480), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003485.1

ORF Insert Sequence:

ATGTCAGGAA TCGCGATTAA ACTCCAACAC AAGCGGGCAC AAGCGGCGGG CATCGGTACC 
AACGCTCAAG CGGTCAAATT CCTTAACCAG GACTATGAAG CCCTCAAACG AGAGTGTCTG 
GAGTCCGGAC GTCTGTTTCA CGATGACATG TTTGAAGCTA ATGTGTCTGC CCTCGGGTTT 
AAGGAACTAG GACCAAACTC GTCTAAAGTG CGCGGGGTTG AGTGGCTGAG ACCCAAACAA 
CTGACGTCCA ACCCAACATT CATTAGTGGA GGAGCGACCA GAACAGACAT CTGCCAGGGC 
GCTTTGGGTG ACTGTTGGCT ATTAGCAGCC ATCGCTTCTC TTACCCTGAA CCAGGACGTG 
TTTGCTCGTG TTGTGCCAGC TGGTCAGAGT TTTGATGGCG ACTATGCTGG AATATTTCAT 
TTTCAGTTTT GGCAGTTTGG TGAATGGGTG GATGTAGTCA TCGATGACCG GCTGCCGGCT 
AGAAACGGAG AGCTGCTGTT TGTCCACTCG GCCGAGGGCT CTGAGTTCTG GAGTGCTCTT 
TTGGAGAAAG CCTATGCAAA GTTGAACGGC TGCTATGAAG CTCTTTCTGG AGGCACCACC 
ACTGAAGGTT TTGAGGATTT CACTGGAGGC ATTGCCGAGG TCCACGAACT GGCCAAAGCA 
GGTCCAAACC TCTTCAAAAC CATCCAGAAG GCTCTTAGCT GGGGCTCGTT GCTGGGCTGC 
TCCATAGATA TCACAAGCTC TTCTGACTCA GAGGCCATTA CTAGTCAGAA ACTGGTGAAA 
GGACACGCCT ACTCGGTTAC AGGAGCTGAA GAGGTGGAGT ACAGGGGTGA CTTAACTAAA 
CTGATTCGAA TCAGGAACCC CTGGGGGCAG GTCGAGTGGA CGGGACCCTG GAGTGATGGG 
TCATCAGAAT GGCGTCAGAT CAGTGACAAC GACAGAGAAA GACTGAGCTC CAAAGCTGAA 
GATGGAGAAT TCTGGATGTC TTTCTCTGAC TTCATGCGTC ATTACTCACG GGTGGAGATC 
TGCAATCTGA CGCCCGATGC GCTCACAGAT GAAAGTGTTA ATAAATGGGC TCTGTCCAAG 
TTTGATGGCA ACTGGAGGAA TGGATCAACT GCTGGAGGCT GCAGGAATTA CCCCAACTCG 
TTCTGGATGA ATCCTCAGTT TCTGATTAAA CTGGAGGAGC AGGATGACGA CCCGACTGAT 
AATGAAGCAG GATGCAGCTT TGTTGTGGGA CTGATCCAGA AGAACCGCCG CAAAATGAGG 
AAGGTCGGAG AGGACATGAA CACCATCGGC TTTGCAATCT ATGAGGTTCC TGACCAGTTT 
GTGGGACAGC GGAACGTCCA TCTGGATCGT AACTTTTTCG TGCGACATGC ATCAGCTGCT 
CGGTCCGAGA CCTTCATCAA CCTGCGGGAG GTTTGCTCTC GCTTCTGTCT GCCCCCCGGC 
GAGTACCTCA TCGTGCCCTC TACTTTTGAG CCCAACAAAG ATGGTGACTT CTGTGTCCGC 
GTTTTCTCAG AAAAACAAGC AGAGTTCCAA GAGCTTGATG ATCCTGTCGA GTCCAAGGTT 
GCGGAGATTG AAATAACAGA GGGTGATATT GATAGCCGCT TCAAGAAACT GTTTGGACAG 
CTTGCTGGAG CTGACTGTGA GATCTCTGCA TTTGAGCTGC AAAAAATCCT GAACAATGTG 
ATAGCTAAAC GCAAAGAGAT TAAAACTGAT GGATTTAGTT TGGAGACTTG CCGCAACATG 
GTGAACCTAC TGGATAAAGA CGGCACTGGA AAACTAGGCC TGCTTGAATT TAAGATTCTG 
TGGACTAAAA TTGAGCTGTT CGTGGACGTC TACAGTAAAA ATGACAAAGA CCAGTCTGGC 
ACAATGAGCT CTATGGAGAT GAGAGAAGCC GTAGAGAAAG CAGGGTTTTC TCTGAATAAT 
GCTCTACACC AGATCCTTGT TGCACGATAC AGCGAATCCA ACCTCACCAT CGACTTTGAC 
AACTTTGTGG CCTGTCTTAT ACGCCTCGAA TGCATGTTCA AGGCATTTAA GGTGCTGGAC 
AAGGACAAGA ATGGCACCGT CGAGCTGAAC ATGATTGAGT GGCTGAATGT TTCCATGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn2l ( NM_001003485.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003485.1 Danio rerio calpain 2, (m/II) large subunit, like (capn2l), mRNA.

Price & Availability↑

CloneID	ODa31394
Clone ID Related Accession (Same CDS sequence)	NM_001003485.1 , NM_001003485.2
Accession Version	NM_001003485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574438400000
Organism	Danio rerio(zebrafish)
Product	calpain-2 catalytic subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR759791.15. On or before Sep 13, 2018 this sequence version replaced NM_001017807.1, NM_001003485.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC093295.1, GDQH01017530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGGAA TCGCGATTAA ACTCCAACAC AAGCGGGCAC AAGCGGCGGG CATCGGTACC 
AACGCTCAAG CGGTCAAATT CCTTAACCAG GACTATGAAG CCCTCAAACG AGAGTGTCTG 
GAGTCCGGAC GTCTGTTTCA CGATGACATG TTTGAAGCTA ATGTGTCTGC CCTCGGGTTT 
AAGGAACTAG GACCAAACTC GTCTAAAGTG CGCGGGGTTG AGTGGCTGAG ACCCAAACAA 
CTGACGTCCA ACCCAACATT CATTAGTGGA GGAGCGACCA GAACAGACAT CTGCCAGGGC 
GCTTTGGGTG ACTGTTGGCT ATTAGCAGCC ATCGCTTCTC TTACCCTGAA CCAGGACGTG 
TTTGCTCGTG TTGTGCCAGC TGGTCAGAGT TTTGATGGCG ACTATGCTGG AATATTTCAT 
TTTCAGTTTT GGCAGTTTGG TGAATGGGTG GATGTAGTCA TCGATGACCG GCTGCCGGCT 
AGAAACGGAG AGCTGCTGTT TGTCCACTCG GCCGAGGGCT CTGAGTTCTG GAGTGCTCTT 
TTGGAGAAAG CCTATGCAAA GTTGAACGGC TGCTATGAAG CTCTTTCTGG AGGCACCACC 
ACTGAAGGTT TTGAGGATTT CACTGGAGGC ATTGCCGAGG TCCACGAACT GGCCAAAGCA 
GGTCCAAACC TCTTCAAAAC CATCCAGAAG GCTCTTAGCT GGGGCTCGTT GCTGGGCTGC 
TCCATAGATA TCACAAGCTC TTCTGACTCA GAGGCCATTA CTAGTCAGAA ACTGGTGAAA 
GGACACGCCT ACTCGGTTAC AGGAGCTGAA GAGGTGGAGT ACAGGGGTGA CTTAACTAAA 
CTGATTCGAA TCAGGAACCC CTGGGGGCAG GTCGAGTGGA CGGGACCCTG GAGTGATGGG 
TCATCAGAAT GGCGTCAGAT CAGTGACAAC GACAGAGAAA GACTGAGCTC CAAAGCTGAA 
GATGGAGAAT TCTGGATGTC TTTCTCTGAC TTCATGCGTC ATTACTCACG GGTGGAGATC 
TGCAATCTGA CGCCCGATGC GCTCACAGAT GAAAGTGTTA ATAAATGGGC TCTGTCCAAG 
TTTGATGGCA ACTGGAGGAA TGGATCAACT GCTGGAGGCT GCAGGAATTA CCCCAACTCG 
TTCTGGATGA ATCCTCAGTT TCTGATTAAA CTGGAGGAGC AGGATGACGA CCCGACTGAT 
AATGAAGCAG GATGCAGCTT TGTTGTGGGA CTGATCCAGA AGAACCGCCG CAAAATGAGG 
AAGGTCGGAG AGGACATGAA CACCATCGGC TTTGCAATCT ATGAGGTTCC TGACCAGTTT 
GTGGGACAGC GGAACGTCCA TCTGGATCGT AACTTTTTCG TGCGACATGC ATCAGCTGCT 
CGGTCCGAGA CCTTCATCAA CCTGCGGGAG GTTTGCTCTC GCTTCTGTCT GCCCCCCGGC 
GAGTACCTCA TCGTGCCCTC TACTTTTGAG CCCAACAAAG ATGGTGACTT CTGTGTCCGC 
GTTTTCTCAG AAAAACAAGC AGAGTTCCAA GAGCTTGATG ATCCTGTCGA GTCCAAGGTT 
GCGGAGATTG AAATAACAGA GGGTGATATT GATAGCCGCT TCAAGAAACT GTTTGGACAG 
CTTGCTGGAG CTGACTGTGA GATCTCTGCA TTTGAGCTGC AAAAAATCCT GAACAATGTG 
ATAGCTAAAC GCAAAGAGAT TAAAACTGAT GGATTTAGTT TGGAGACTTG CCGCAACATG 
GTGAACCTAC TGGATAAAGA CGGCACTGGA AAACTAGGCC TGCTTGAATT TAAGATTCTG 
TGGACTAAAA TTGAGCTGTT CGTGGACGTC TACAGTAAAA ATGACAAAGA CCAGTCTGGC 
ACAATGAGCT CTATGGAGAT GAGAGAAGCC GTAGAGAAAG CAGGGTTTTC TCTGAATAAT 
GCTCTACACC AGATCCTTGT TGCACGATAC AGCGAATCCA ACCTCACCAT CGACTTTGAC 
AACTTTGTGG CCTGTCTTAT ACGCCTCGAA TGCATGTTCA AGGCATTTAA GGTGCTGGAC 
AAGGACAAGA ATGGCACCGT CGAGCTGAAC ATGATTGAGT GGCTGAATGT TTCCATGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003485.1
CDS	35..2137
Translation	MSGIAIKLQHKRAQAAGIGTNAQAVKFLNQDYEALKRECLESGRLFHDDMFEANVSALGFKELGPNSSKVRGVEWLRPKQLTSNPTFISGGATRTDICQGALGDCWLLAAIASLTLNQDVFARVVPAGQSFDGDYAGIFHFQFWQFGEWVDVVIDDRLPARNGELLFVHSAEGSEFWSALLEKAYAKLNGCYEALSGGTTTEGFEDFTGGIAEVHELAKAGPNLFKTIQKALSWGSLLGCSIDITSSSDSEAITSQKLVKGHAYSVTGAEEVEYRGDLTKLIRIRNPWGQVEWTGPWSDGSSEWRQISDNDRERLSSKAEDGEFWMSFSDFMRHYSRVEICNLTPDALTDESVNKWALSKFDGNWRNGSTAGGCRNYPNSFWMNPQFLIKLEEQDDDPTDNEAGCSFVVGLIQKNRRKMRKVGEDMNTIGFAIYEVPDQFVGQRNVHLDRNFFVRHASAARSETFINLREVCSRFCLPPGEYLIVPSTFEPNKDGDFCVRVFSEKQAEFQELDDPVESKVAEIEITEGDIDSRFKKLFGQLAGADCEISAFELQKILNNVIAKRKEIKTDGFSLETCRNMVNLLDKDGTGKLGLLEFKILWTKIELFVDVYSKNDKDQSGTMSSMEMREAVEKAGFSLNNALHQILVARYSESNLTIDFDNFVACLIRLECMFKAFKVLDKDKNGTVELNMIEWLNVSML

Target ORF information:

RefSeq Version	NM_001003485.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 2, (m/II) large subunit, like (capn2l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003485.2

ORF Insert Sequence:

ATGTCAGGAA TCGCGATTAA ACTCCAACAC AAGCGGGCAC AAGCGGCGGG CATCGGTACC 
AACGCTCAAG CGGTCAAATT CCTTAACCAG GACTATGAAG CCCTCAAACG AGAGTGTCTG 
GAGTCCGGAC GTCTGTTTCA CGATGACATG TTTGAAGCTA ATGTGTCTGC CCTCGGGTTT 
AAGGAACTAG GACCAAACTC GTCTAAAGTG CGCGGGGTTG AGTGGCTGAG ACCCAAACAA 
CTGACGTCCA ACCCAACATT CATTAGTGGA GGAGCGACCA GAACAGACAT CTGCCAGGGC 
GCTTTGGGTG ACTGTTGGCT ATTAGCAGCC ATCGCTTCTC TTACCCTGAA CCAGGACGTG 
TTTGCTCGTG TTGTGCCAGC TGGTCAGAGT TTTGATGGCG ACTATGCTGG AATATTTCAT 
TTTCAGTTTT GGCAGTTTGG TGAATGGGTG GATGTAGTCA TCGATGACCG GCTGCCGGCT 
AGAAACGGAG AGCTGCTGTT TGTCCACTCG GCCGAGGGCT CTGAGTTCTG GAGTGCTCTT 
TTGGAGAAAG CCTATGCAAA GTTGAACGGC TGCTATGAAG CTCTTTCTGG AGGCACCACC 
ACTGAAGGTT TTGAGGATTT CACTGGAGGC ATTGCCGAGG TCCACGAACT GGCCAAAGCA 
GGTCCAAACC TCTTCAAAAC CATCCAGAAG GCTCTTAGCT GGGGCTCGTT GCTGGGCTGC 
TCCATAGATA TCACAAGCTC TTCTGACTCA GAGGCCATTA CTAGTCAGAA ACTGGTGAAA 
GGACACGCCT ACTCGGTTAC AGGAGCTGAA GAGGTGGAGT ACAGGGGTGA CTTAACTAAA 
CTGATTCGAA TCAGGAACCC CTGGGGGCAG GTCGAGTGGA CGGGACCCTG GAGTGATGGG 
TCATCAGAAT GGCGTCAGAT CAGTGACAAC GACAGAGAAA GACTGAGCTC CAAAGCTGAA 
GATGGAGAAT TCTGGATGTC TTTCTCTGAC TTCATGCGTC ATTACTCACG GGTGGAGATC 
TGCAATCTGA CGCCCGATGC GCTCACAGAT GAAAGTGTTA ATAAATGGGC TCTGTCCAAG 
TTTGATGGCA ACTGGAGGAA TGGATCAACT GCTGGAGGCT GCAGGAATTA CCCCAACTCG 
TTCTGGATGA ATCCTCAGTT TCTGATTAAA CTGGAGGAGC AGGATGACGA CCCGACTGAT 
AATGAAGCAG GATGCAGCTT TGTTGTGGGA CTGATCCAGA AGAACCGCCG CAAAATGAGG 
AAGGTCGGAG AGGACATGAA CACCATCGGC TTTGCAATCT ATGAGGTTCC TGACCAGTTT 
GTGGGACAGC GGAACGTCCA TCTGGATCGT AACTTTTTCG TGCGACATGC ATCAGCTGCT 
CGGTCCGAGA CCTTCATCAA CCTGCGGGAG GTTTGCTCTC GCTTCTGTCT GCCCCCCGGC 
GAGTACCTCA TCGTGCCCTC TACTTTTGAG CCCAACAAAG ATGGTGACTT CTGTGTCCGC 
GTTTTCTCAG AAAAACAAGC AGAGTTCCAA GAGCTTGATG ATCCTGTCGA GTCCAAGGTT 
GCGGAGATTG AAATAACAGA GGGTGATATT GATAGCCGCT TCAAGAAACT GTTTGGACAG 
CTTGCTGGAG CTGACTGTGA GATCTCTGCA TTTGAGCTGC AAAAAATCCT GAACAATGTG 
ATAGCTAAAC GCAAAGAGAT TAAAACTGAT GGATTTAGTT TGGAGACTTG CCGCAACATG 
GTGAACCTAC TGGATAAAGA CGGCACTGGA AAACTAGGCC TGCTTGAATT TAAGATTCTG 
TGGACTAAAA TTGAGCTGTT CGTGGACGTC TACAGTAAAA ATGACAAAGA CCAGTCTGGC 
ACAATGAGCT CTATGGAGAT GAGAGAAGCC GTAGAGAAAG CAGGGTTTTC TCTGAATAAT 
GCTCTACACC AGATCCTTGT TGCACGATAC AGCGAATCCA ACCTCACCAT CGACTTTGAC 
AACTTTGTGG CCTGTCTTAT ACGCCTCGAA TGCATGTTCA AGGCATTTAA GGTGCTGGAC 
AAGGACAAGA ATGGCACCGT CGAGCTGAAC ATGATTGAGT GGCTGAATGT TTCCATGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vascular defects of
Disease models & mechanisms12(5)(2019 05)
Cho HJ,Lee JG,Kim JH,Kim SY,Huh YH,Kim HJ,Lee KS,Yu K,Lee JS

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
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