Capn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Capn2
Entrez Gene ID	12334
Full Name	calpain 2
Synonyms	AI326419,Capa-2,Capa2,m-calpain,m-calpin
General protein information	Preferred Names calpain 2 Names calpain-2 catalytic subunit 80 kDa M-calpain subunit CALP80 CANP 2 calcium-activated neutral proteinase 2 calpain M-type calpain-2 large subunit cysteine proteinase m-calpain 80 kDa large subunit m-calpain large subunit millimolar-calpain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H5

mRNA and Protein(s)

mRNA	Protein	Name
NM_009794.4	NP_033924.2	calpain-2 catalytic subunit
NM_009794.3	NP_033924.2	calpain-2 catalytic subunit

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CAPN2	XP_001098172.1
Mus musculus (house mouse)	Capn2	NP_033924.2
Danio rerio (zebrafish)	zgc:92480	NP_001003485.1
Rattus norvegicus (Norway rat)	Capn2	NP_058812.1
Xenopus tropicalis (tropical clawed frog)	capn2.2	NP_001005446.1
Homo sapiens (human)	CAPN2	NP_001739.2
Pan troglodytes (chimpanzee)	CAPN2	XP_003308813.1
Danio rerio (zebrafish)	capn2l	NP_001017807.1
Canis lupus familiaris (dog)	CAPN2	XP_005622713.1
Bos taurus (cattle)	CAPN2	NP_001096556.1
Gallus gallus (chicken)	CAPN2	NP_990411.1

Gene Ontology
Bibliography

Related articles in PubMed

Isoform-specific hyperactivation of calpain-2 occurs presymptomatically at the synapse in Alzheimer's disease mice and correlates with memory deficits in human subjects.
Ahmad F, Das D, Kommaddi RP, Diwakar L, Gowaikar R, Rupanagudi KV, Bennett DA, Ravindranath V
Scientific reports8(1)13119(2018 09)

Early CALP2 expression and microglial activation are potential inducers of spinal IL-6 up-regulation and bilateral pain following motor nerve injury.
Chen SX, Wang SK, Yao PW, Liao GJ, Na XD, Li YY, Zeng WA, Liu XG, Zang Y
Journal of neurochemistry145(2)154-169(2018 04)

Protection against TBI-Induced Neuronal Death with Post-Treatment with a Selective Calpain-2 Inhibitor in Mice.
Wang Y, Liu Y, Lopez D, Lee M, Dayal S, Hurtado A, Bi X, Baudry M
Journal of neurotrauma35(1)105-117(2018 01)

Hippocampal calpain is required for the consolidation and reconsolidation but not extinction of contextual fear memory.
Nagayoshi T, Isoda K, Mamiya N, Kida S
Molecular brain10(1)61(2017 Dec)

The tyrosine phosphatase PTPN13/FAP-1 links calpain-2, TBI and tau tyrosine phosphorylation.
Wang Y, Hall RA, Lee M, Kamgar-Parsi A, Bi X, Baudry M
Scientific reports7(1)11771(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Capn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18228	NM_009794.4 Latest version!	Mus musculus calpain 2 (Capn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$391.30 $559.00
View Old Accession Versions >>
OMu18228	NM_009794.3	Mus musculus calpain 2 (Capn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Capn2 ( NM_009794.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033924.2 Mus musculus calpain 2 (Capn2), mRNA.

Price & Availability↑

CloneID	OMu18228
Clone ID Related Accession (Same CDS sequence)	NM_009794.4 , NM_009794.3
Accession Version	NM_009794.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509465600000
Organism	Mus musculus(house mouse)
Product	calpain-2 catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054726.1. On Oct 6, 2007 this sequence version replaced NM_009794.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054726.1, AK154855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCA TCGCGATAAA GCTGGCCAAG GACCGCGAAG CGGCCGAGGG GCTGGGGTCG 
CATGAGAGAG CCATCAAGTA CCTCAACCAG GACTACGAGA CGCTGCGGAA CGAGTGCCTG 
GAGGCCGGGG CGCTCTTCCA GGATCCTTCC TTCCCCGCCC TGCCGTCATC CTTGGGCTAT 
AAGGAGTTGG GGCCCTACTC CAGCAAAACT CGGGGCATCG AATGGAAGCG GCCCACGGAG 
ATCTGCGCTG ACCCCCAGTT CATTATTGGA GGAGCCACCC GCACAGACAT CTGCCAAGGA 
GCCCTGGGGG ACTGCTGGCT GCTGGCTGCC ATCGCCTCCC TCACCTTGAA TGAGGAAATC 
CTGGCTCGGG TTGTGCCTCC AGATCAGAGC TTCCAGGAAA ACTATGCAGG CATCTTCCAC 
TTCCAGTTCT GGCAATATGG CGAGTGGGTG GAGGTGGTGG TGGACGACAG GCTGCCCACT 
AAGGACGGGG AGCTGCTCTT TGTGCATTCA GCTGAAGGGA GTGAGTTCTG GAGTGCCCTT 
CTGGAGAAAG CCTATGCCAA GATCAATGGG TGCTATGAAG CACTCTCAGG GGGTGCCACC 
ACGGAAGGCT TTGAAGACTT CACAGGAGGC ATTGCTGAGT GGTATGAGCT GAGGAAGCCT 
CCTCCCAACT TGTTCAAGAT CATCCAGAAG GCTTTGGAGA AAGGTTCTCT GCTTGGCTGC 
TCTATCGATA TCACCAGCGC TGCAGATTCT GAGGCCGTTA CGTACCAGAA GTTGGTGAAA 
GGACATGCGT ACTCTGTCAC CGGAGCCGAG GAGGTTGAAA GTTCAGGAAG CCTGCAGAAA 
TTGATCCGCA TCAGGAATCC CTGGGGACAA GTAGAGTGGA CCGGGAAGTG GAATGACAAT 
TGCCCCAGCT GGAACACGGT TGACCCAGAA GTAAGGGCAA ATCTAACCGA ACGGCAGGAG 
GACGGAGAAT TCTGGATGTC CTTCAGTGAC TTCCTGAGAC ACTACTCTCG CCTGGAGATC 
TGCAACCTGA CCCCGGACAC CCTCACCTGT GACTCCTATA AGAAGTGGAA ACTCACCAAG 
ATGGATGGGA ACTGGAGGCG AGGCTCCACC GCAGGGGGCT GCAGGAATTA CCCAAATACC 
TTCTGGATGA ACCCTCAGTA CCTAATTAAG CTGGAGGAAG AAGACGAAGA TGAGGAGGAT 
GGGGAGAGAG GCTGTACCTT CCTGGTGGGT CTCATCCAGA AGCACCGGCG ACGGCAGAGG 
AAGATGGGCG AGGACATGCA CACCATTGGC TTCGGCATCT ATGAGGTTCC AGAGGAGCTA 
ACAGGGCAGA CCAACATCCA CCTCGGCAAA AACTTTTTCC TCACAACCCG AGCCAGGGAG 
CGGTCAGATA CCTTCATTAA CCTCCGCGAG GTCCTCAACC GCTTCAAGCT GCCCCCGGGA 
GAGTATGTCC TCGTTCCCTC CACCTTCGAA CCCCACAAGG ATGGCGATTT CTGCATCCGA 
GTCTTCTCGG AGAAGAAGGC TGACTACCAA GCTGTTGATG ACGAGATCGA GGCCAACATT 
GAAGAGATTG ACGCCAATGA GGAGGACATT GACGATGGAT TCCGAAGGCT CTTTGTTCAG 
CTGGCTGGAG AGGATGCAGA GATCTCTGCC TTTGAGCTGC AGACCATCTT AAGAAGAGTT 
CTAGCCAAAC GCCAAGACAT CAAGTCAGAC GGCTTCAGCA TCGAGACCTG TAAGATCATG 
GTGGACATGC TGGATGAAGA TGGGAGTGGC AAGCTTGGCC TGAAGGAGTT CTACATCCTC 
TGGACGAAGA TTCAGAAATA CCAAAAAATC TACCGGGAAA TCGATGTGGA CAGGTCTGGA 
ACCATGAATT CCTACGAGAT GCGGAAAGCA CTGGAAGAAG CAGGTTTCAA GCTGCCCTGT 
CAACTTCATC AAGTCATCGT TGCCCGGTTT GCAGACGACG AGCTAATCAT CGACTTTGAC 
AATTTTGTGC GGTGTTTGGT CCGTCTGGAA ACGCTATTCA AGATATTCAA GCAGCTAGAC 
CCTGAGAACA CTGGAACGAT ACAGCTCAAC CTTGCTTCGT GGCTGAGTTT TTCAGTACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033924.2
CDS	130..2232
Translation	MAGIAIKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDPSFPALPSSLGYKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPPDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELRKPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSGSLQKLIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRHYSRLEICNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGERGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELTGQTNIHLGKNFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFEPHKDGDFCIRVFSEKKADYQAVDDEIEANIEEIDANEEDIDDGFRRLFVQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIQLNLASWLSFSVL

Target ORF information:

RefSeq Version	NM_009794.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpain 2 (Capn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009794.3

ORF Insert Sequence:

ATGGCGGGCA TCGCGATAAA GCTGGCCAAG GACCGCGAAG CGGCCGAGGG GCTGGGGTCG 
CATGAGAGAG CCATCAAGTA CCTCAACCAG GACTACGAGA CGCTGCGGAA CGAGTGCCTG 
GAGGCCGGGG CGCTCTTCCA GGATCCTTCC TTCCCCGCCC TGCCGTCATC CTTGGGCTAT 
AAGGAGTTGG GGCCCTACTC CAGCAAAACT CGGGGCATCG AATGGAAGCG GCCCACGGAG 
ATCTGCGCTG ACCCCCAGTT CATTATTGGA GGAGCCACCC GCACAGACAT CTGCCAAGGA 
GCCCTGGGGG ACTGCTGGCT GCTGGCTGCC ATCGCCTCCC TCACCTTGAA TGAGGAAATC 
CTGGCTCGGG TTGTGCCTCC AGATCAGAGC TTCCAGGAAA ACTATGCAGG CATCTTCCAC 
TTCCAGTTCT GGCAATATGG CGAGTGGGTG GAGGTGGTGG TGGACGACAG GCTGCCCACT 
AAGGACGGGG AGCTGCTCTT TGTGCATTCA GCTGAAGGGA GTGAGTTCTG GAGTGCCCTT 
CTGGAGAAAG CCTATGCCAA GATCAATGGG TGCTATGAAG CACTCTCAGG GGGTGCCACC 
ACGGAAGGCT TTGAAGACTT CACAGGAGGC ATTGCTGAGT GGTATGAGCT GAGGAAGCCT 
CCTCCCAACT TGTTCAAGAT CATCCAGAAG GCTTTGGAGA AAGGTTCTCT GCTTGGCTGC 
TCTATCGATA TCACCAGCGC TGCAGATTCT GAGGCCGTTA CGTACCAGAA GTTGGTGAAA 
GGACATGCGT ACTCTGTCAC CGGAGCCGAG GAGGTTGAAA GTTCAGGAAG CCTGCAGAAA 
TTGATCCGCA TCAGGAATCC CTGGGGACAA GTAGAGTGGA CCGGGAAGTG GAATGACAAT 
TGCCCCAGCT GGAACACGGT TGACCCAGAA GTAAGGGCAA ATCTAACCGA ACGGCAGGAG 
GACGGAGAAT TCTGGATGTC CTTCAGTGAC TTCCTGAGAC ACTACTCTCG CCTGGAGATC 
TGCAACCTGA CCCCGGACAC CCTCACCTGT GACTCCTATA AGAAGTGGAA ACTCACCAAG 
ATGGATGGGA ACTGGAGGCG AGGCTCCACC GCAGGGGGCT GCAGGAATTA CCCAAATACC 
TTCTGGATGA ACCCTCAGTA CCTAATTAAG CTGGAGGAAG AAGACGAAGA TGAGGAGGAT 
GGGGAGAGAG GCTGTACCTT CCTGGTGGGT CTCATCCAGA AGCACCGGCG ACGGCAGAGG 
AAGATGGGCG AGGACATGCA CACCATTGGC TTCGGCATCT ATGAGGTTCC AGAGGAGCTA 
ACAGGGCAGA CCAACATCCA CCTCGGCAAA AACTTTTTCC TCACAACCCG AGCCAGGGAG 
CGGTCAGATA CCTTCATTAA CCTCCGCGAG GTCCTCAACC GCTTCAAGCT GCCCCCGGGA 
GAGTATGTCC TCGTTCCCTC CACCTTCGAA CCCCACAAGG ATGGCGATTT CTGCATCCGA 
GTCTTCTCGG AGAAGAAGGC TGACTACCAA GCTGTTGATG ACGAGATCGA GGCCAACATT 
GAAGAGATTG ACGCCAATGA GGAGGACATT GACGATGGAT TCCGAAGGCT CTTTGTTCAG 
CTGGCTGGAG AGGATGCAGA GATCTCTGCC TTTGAGCTGC AGACCATCTT AAGAAGAGTT 
CTAGCCAAAC GCCAAGACAT CAAGTCAGAC GGCTTCAGCA TCGAGACCTG TAAGATCATG 
GTGGACATGC TGGATGAAGA TGGGAGTGGC AAGCTTGGCC TGAAGGAGTT CTACATCCTC 
TGGACGAAGA TTCAGAAATA CCAAAAAATC TACCGGGAAA TCGATGTGGA CAGGTCTGGA 
ACCATGAATT CCTACGAGAT GCGGAAAGCA CTGGAAGAAG CAGGTTTCAA GCTGCCCTGT 
CAACTTCATC AAGTCATCGT TGCCCGGTTT GCAGACGACG AGCTAATCAT CGACTTTGAC 
AATTTTGTGC GGTGTTTGGT CCGTCTGGAA ACGCTATTCA AGATATTCAA GCAGCTAGAC 
CCTGAGAACA CTGGAACGAT ACAGCTCAAC CTTGCTTCGT GGCTGAGTTT TTCAGTACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn2 ( NM_009794.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033924.2 Mus musculus calpain 2 (Capn2), mRNA.

Price & Availability↑

CloneID	OMu18228
Clone ID Related Accession (Same CDS sequence)	NM_009794.4 , NM_009794.3
Accession Version	NM_009794.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Mus musculus(house mouse)
Product	calpain-2 catalytic subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131742.2, BC054726.1 and BG091141.1. On Feb 13, 2018 this sequence version replaced NM_009794.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.125795.1, D38117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGGGCA TCGCGATAAA GCTGGCCAAG GACCGCGAAG CGGCCGAGGG GCTGGGGTCG 
CATGAGAGAG CCATCAAGTA CCTCAACCAG GACTACGAGA CGCTGCGGAA CGAGTGCCTG 
GAGGCCGGGG CGCTCTTCCA GGATCCTTCC TTCCCCGCCC TGCCGTCATC CTTGGGCTAT 
AAGGAGTTGG GGCCCTACTC CAGCAAAACT CGGGGCATCG AATGGAAGCG GCCCACGGAG 
ATCTGCGCTG ACCCCCAGTT CATTATTGGA GGAGCCACCC GCACAGACAT CTGCCAAGGA 
GCCCTGGGGG ACTGCTGGCT GCTGGCTGCC ATCGCCTCCC TCACCTTGAA TGAGGAAATC 
CTGGCTCGGG TTGTGCCTCC AGATCAGAGC TTCCAGGAAA ACTATGCAGG CATCTTCCAC 
TTCCAGTTCT GGCAATATGG CGAGTGGGTG GAGGTGGTGG TGGACGACAG GCTGCCCACT 
AAGGACGGGG AGCTGCTCTT TGTGCATTCA GCTGAAGGGA GTGAGTTCTG GAGTGCCCTT 
CTGGAGAAAG CCTATGCCAA GATCAATGGG TGCTATGAAG CACTCTCAGG GGGTGCCACC 
ACGGAAGGCT TTGAAGACTT CACAGGAGGC ATTGCTGAGT GGTATGAGCT GAGGAAGCCT 
CCTCCCAACT TGTTCAAGAT CATCCAGAAG GCTTTGGAGA AAGGTTCTCT GCTTGGCTGC 
TCTATCGATA TCACCAGCGC TGCAGATTCT GAGGCCGTTA CGTACCAGAA GTTGGTGAAA 
GGACATGCGT ACTCTGTCAC CGGAGCCGAG GAGGTTGAAA GTTCAGGAAG CCTGCAGAAA 
TTGATCCGCA TCAGGAATCC CTGGGGACAA GTAGAGTGGA CCGGGAAGTG GAATGACAAT 
TGCCCCAGCT GGAACACGGT TGACCCAGAA GTAAGGGCAA ATCTAACCGA ACGGCAGGAG 
GACGGAGAAT TCTGGATGTC CTTCAGTGAC TTCCTGAGAC ACTACTCTCG CCTGGAGATC 
TGCAACCTGA CCCCGGACAC CCTCACCTGT GACTCCTATA AGAAGTGGAA ACTCACCAAG 
ATGGATGGGA ACTGGAGGCG AGGCTCCACC GCAGGGGGCT GCAGGAATTA CCCAAATACC 
TTCTGGATGA ACCCTCAGTA CCTAATTAAG CTGGAGGAAG AAGACGAAGA TGAGGAGGAT 
GGGGAGAGAG GCTGTACCTT CCTGGTGGGT CTCATCCAGA AGCACCGGCG ACGGCAGAGG 
AAGATGGGCG AGGACATGCA CACCATTGGC TTCGGCATCT ATGAGGTTCC AGAGGAGCTA 
ACAGGGCAGA CCAACATCCA CCTCGGCAAA AACTTTTTCC TCACAACCCG AGCCAGGGAG 
CGGTCAGATA CCTTCATTAA CCTCCGCGAG GTCCTCAACC GCTTCAAGCT GCCCCCGGGA 
GAGTATGTCC TCGTTCCCTC CACCTTCGAA CCCCACAAGG ATGGCGATTT CTGCATCCGA 
GTCTTCTCGG AGAAGAAGGC TGACTACCAA GCTGTTGATG ACGAGATCGA GGCCAACATT 
GAAGAGATTG ACGCCAATGA GGAGGACATT GACGATGGAT TCCGAAGGCT CTTTGTTCAG 
CTGGCTGGAG AGGATGCAGA GATCTCTGCC TTTGAGCTGC AGACCATCTT AAGAAGAGTT 
CTAGCCAAAC GCCAAGACAT CAAGTCAGAC GGCTTCAGCA TCGAGACCTG TAAGATCATG 
GTGGACATGC TGGATGAAGA TGGGAGTGGC AAGCTTGGCC TGAAGGAGTT CTACATCCTC 
TGGACGAAGA TTCAGAAATA CCAAAAAATC TACCGGGAAA TCGATGTGGA CAGGTCTGGA 
ACCATGAATT CCTACGAGAT GCGGAAAGCA CTGGAAGAAG CAGGTTTCAA GCTGCCCTGT 
CAACTTCATC AAGTCATCGT TGCCCGGTTT GCAGACGACG AGCTAATCAT CGACTTTGAC 
AATTTTGTGC GGTGTTTGGT CCGTCTGGAA ACGCTATTCA AGATATTCAA GCAGCTAGAC 
CCTGAGAACA CTGGAACGAT ACAGCTCAAC CTTGCTTCGT GGCTGAGTTT TTCAGTACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033924.2
CDS	257..2359
Translation	MAGIAIKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDPSFPALPSSLGYKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPPDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELRKPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSGSLQKLIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRHYSRLEICNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGERGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELTGQTNIHLGKNFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFEPHKDGDFCIRVFSEKKADYQAVDDEIEANIEEIDANEEDIDDGFRRLFVQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIQLNLASWLSFSVL

Target ORF information:

RefSeq Version	NM_009794.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpain 2 (Capn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009794.4

ORF Insert Sequence:

ATGGCGGGCA TCGCGATAAA GCTGGCCAAG GACCGCGAAG CGGCCGAGGG GCTGGGGTCG 
CATGAGAGAG CCATCAAGTA CCTCAACCAG GACTACGAGA CGCTGCGGAA CGAGTGCCTG 
GAGGCCGGGG CGCTCTTCCA GGATCCTTCC TTCCCCGCCC TGCCGTCATC CTTGGGCTAT 
AAGGAGTTGG GGCCCTACTC CAGCAAAACT CGGGGCATCG AATGGAAGCG GCCCACGGAG 
ATCTGCGCTG ACCCCCAGTT CATTATTGGA GGAGCCACCC GCACAGACAT CTGCCAAGGA 
GCCCTGGGGG ACTGCTGGCT GCTGGCTGCC ATCGCCTCCC TCACCTTGAA TGAGGAAATC 
CTGGCTCGGG TTGTGCCTCC AGATCAGAGC TTCCAGGAAA ACTATGCAGG CATCTTCCAC 
TTCCAGTTCT GGCAATATGG CGAGTGGGTG GAGGTGGTGG TGGACGACAG GCTGCCCACT 
AAGGACGGGG AGCTGCTCTT TGTGCATTCA GCTGAAGGGA GTGAGTTCTG GAGTGCCCTT 
CTGGAGAAAG CCTATGCCAA GATCAATGGG TGCTATGAAG CACTCTCAGG GGGTGCCACC 
ACGGAAGGCT TTGAAGACTT CACAGGAGGC ATTGCTGAGT GGTATGAGCT GAGGAAGCCT 
CCTCCCAACT TGTTCAAGAT CATCCAGAAG GCTTTGGAGA AAGGTTCTCT GCTTGGCTGC 
TCTATCGATA TCACCAGCGC TGCAGATTCT GAGGCCGTTA CGTACCAGAA GTTGGTGAAA 
GGACATGCGT ACTCTGTCAC CGGAGCCGAG GAGGTTGAAA GTTCAGGAAG CCTGCAGAAA 
TTGATCCGCA TCAGGAATCC CTGGGGACAA GTAGAGTGGA CCGGGAAGTG GAATGACAAT 
TGCCCCAGCT GGAACACGGT TGACCCAGAA GTAAGGGCAA ATCTAACCGA ACGGCAGGAG 
GACGGAGAAT TCTGGATGTC CTTCAGTGAC TTCCTGAGAC ACTACTCTCG CCTGGAGATC 
TGCAACCTGA CCCCGGACAC CCTCACCTGT GACTCCTATA AGAAGTGGAA ACTCACCAAG 
ATGGATGGGA ACTGGAGGCG AGGCTCCACC GCAGGGGGCT GCAGGAATTA CCCAAATACC 
TTCTGGATGA ACCCTCAGTA CCTAATTAAG CTGGAGGAAG AAGACGAAGA TGAGGAGGAT 
GGGGAGAGAG GCTGTACCTT CCTGGTGGGT CTCATCCAGA AGCACCGGCG ACGGCAGAGG 
AAGATGGGCG AGGACATGCA CACCATTGGC TTCGGCATCT ATGAGGTTCC AGAGGAGCTA 
ACAGGGCAGA CCAACATCCA CCTCGGCAAA AACTTTTTCC TCACAACCCG AGCCAGGGAG 
CGGTCAGATA CCTTCATTAA CCTCCGCGAG GTCCTCAACC GCTTCAAGCT GCCCCCGGGA 
GAGTATGTCC TCGTTCCCTC CACCTTCGAA CCCCACAAGG ATGGCGATTT CTGCATCCGA 
GTCTTCTCGG AGAAGAAGGC TGACTACCAA GCTGTTGATG ACGAGATCGA GGCCAACATT 
GAAGAGATTG ACGCCAATGA GGAGGACATT GACGATGGAT TCCGAAGGCT CTTTGTTCAG 
CTGGCTGGAG AGGATGCAGA GATCTCTGCC TTTGAGCTGC AGACCATCTT AAGAAGAGTT 
CTAGCCAAAC GCCAAGACAT CAAGTCAGAC GGCTTCAGCA TCGAGACCTG TAAGATCATG 
GTGGACATGC TGGATGAAGA TGGGAGTGGC AAGCTTGGCC TGAAGGAGTT CTACATCCTC 
TGGACGAAGA TTCAGAAATA CCAAAAAATC TACCGGGAAA TCGATGTGGA CAGGTCTGGA 
ACCATGAATT CCTACGAGAT GCGGAAAGCA CTGGAAGAAG CAGGTTTCAA GCTGCCCTGT 
CAACTTCATC AAGTCATCGT TGCCCGGTTT GCAGACGACG AGCTAATCAT CGACTTTGAC 
AATTTTGTGC GGTGTTTGGT CCGTCTGGAA ACGCTATTCA AGATATTCAA GCAGCTAGAC 
CCTGAGAACA CTGGAACGAT ACAGCTCAAC CTTGCTTCGT GGCTGAGTTT TTCAGTACTC 
TGA