lum cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lum
Entrez Gene ID	415149
Full Name	lumican
Synonyms	zgc:86661
General protein information	Preferred Names lumican Names lumican zlum
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002059.1	NP_001002059.1	lumican precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	lum	NP_001011006.1
Homo sapiens (human)	LUM	NP_002336.1
Macaca mulatta (Rhesus monkey)	LUM	XP_001102667.1
Bos taurus (cattle)	LUM	NP_776359.1
Danio rerio (zebrafish)	lum	NP_001002059.1
Mus musculus (house mouse)	Lum	NP_032550.2
Pan troglodytes (chimpanzee)	LUM	XP_509260.3
Canis lupus familiaris (dog)	LUM	XP_539716.1
Rattus norvegicus (Norway rat)	Lum	NP_112312.1
Gallus gallus (chicken)	LUM	NP_001263286.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Partial depletion of yolk during zebrafish embryogenesis changes the dynamics of methionine cycle and metabolic genes.
Huang Y, Linsen SE
BMC genomics16427(2015 Jun)

Global protein expression profiling of zebrafish organs based on in vivo incorporation of stable isotopes.
Nolte H, Konzer A, Ruhs A, Jungblut B, Braun T, Krüger M
Journal of proteome research13(4)2162-74(2014 Apr)

Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung.
Zheng W, Wang Z, Collins JE, Andrews RM, Stemple D, Gong Z
PloS one6(8)e24019(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lum gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lum cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31547	NM_001002059.1 Latest version!	Danio rerio lumican (lum), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lum ( NM_001002059.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002059.1 Danio rerio lumican (lum), mRNA.

Price & Availability↑

CloneID	ODa31547
Clone ID Related Accession (Same CDS sequence)	NM_001002059.1
Accession Version	NM_001002059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	lumican precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071347.1. ##Evidence-Data-START## Transcript exon combination :: BC071347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476733, SAMEA4476738 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGCTC TGGGATCCAT CCTGTTGGCT GGACTTCTAA GTCTGAGTTT AGCTCAGTAT 
GATTACTATG ATGAGTACTA CATACCCTCT GCACCACTGG AGGGCGTTTC ATCACCTTCT 
TGTGCCCAGG AGTGCGAATG TCCCATCAAC TTTCCCACTG CCATGTACTG CAATGAGCGC 
AACTTGAAGT TCATCCCCAT CGTGCCGACC GGAATCAAAT ACCTCTACTT ACAGAACAAC 
TTCATTGAGG AGATCAAGGC TGGAGTGTTT GACAATGCCA CCGACCTTCG CTGGCTCGTC 
CTCGACAACA ACAACATCAC CAGTGACAAG ATCCAGGCTG GTACTATCGA TAAACTAGGG 
AGCTTGGAGA AGCTCCTCTT CAGTCATAAC AAGCTGACTA AACCCCCTGG TTCTCTTTCG 
AAGTCTCTGG ATGAGTTGAA GTTGATCGGT AACAAGTTAA CCTCATTCCC TGCTAACACT 
TTGGCTGGGA TGGAAAACCT GACTACCGTC CACTTGTCCA AGAACAAGCT TACCACCGAG 
AGCCTCACAG GAGCCTTCAA GGGCCTCAAG TCCCTCATTC TGCTGGACGT GAGCGAGAAC 
AAGCTCAAGA AACTTCCCTC GGGAGTTCCA GCTTCGCTTT TGATGCTCTA CGCTGACAAT 
AATGACATCG ACAGCATCCC TAACGGCTAC CTGGCAAAGC TGCCACTCCT ACAGTACCTG 
CGCATCTCCC ACAACAAACT TGTAGACTCA GGGGTCCCTG CAGGAGTGTT CAATGTGTCC 
TCTCTTCTTG AGCTTGACCT GTCTTTCAAC AAGCTGAAGA CCATCCCGGA GATCAACGAA 
TCACTAGAAC ACCTCTACCT GCAAGTCAAT GAGATAAACA AGTTTGAGCT GACGAATATC 
TGCAGGTTTA GCTCACCGGT CAACTACTCT AGACTGAGGA CCCTGCGCTT GGATGGAAAC 
AACATCACAC ACAGCAGTAT GCCAGATGAC ACAGCTAACT GCCTACGCCA GGCCTCAGAG 
ATCATCTTTG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002059.1
CDS	59..1093
Translation	MFALGSILLAGLLSLSLAQYDYYDEYYIPSAPLEGVSSPSCAQECECPINFPTAMYCNERNLKFIPIVPTGIKYLYLQNNFIEEIKAGVFDNATDLRWLVLDNNNITSDKIQAGTIDKLGSLEKLLFSHNKLTKPPGSLSKSLDELKLIGNKLTSFPANTLAGMENLTTVHLSKNKLTTESLTGAFKGLKSLILLDVSENKLKKLPSGVPASLLMLYADNNDIDSIPNGYLAKLPLLQYLRISHNKLVDSGVPAGVFNVSSLLELDLSFNKLKTIPEINESLEHLYLQVNEINKFELTNICRFSSPVNYSRLRTLRLDGNNITHSSMPDDTANCLRQASEIIFE

Target ORF information:

RefSeq Version	NM_001002059.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lumican (lum), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002059.1

ORF Insert Sequence:

ATGTTTGCTC TGGGATCCAT CCTGTTGGCT GGACTTCTAA GTCTGAGTTT AGCTCAGTAT 
GATTACTATG ATGAGTACTA CATACCCTCT GCACCACTGG AGGGCGTTTC ATCACCTTCT 
TGTGCCCAGG AGTGCGAATG TCCCATCAAC TTTCCCACTG CCATGTACTG CAATGAGCGC 
AACTTGAAGT TCATCCCCAT CGTGCCGACC GGAATCAAAT ACCTCTACTT ACAGAACAAC 
TTCATTGAGG AGATCAAGGC TGGAGTGTTT GACAATGCCA CCGACCTTCG CTGGCTCGTC 
CTCGACAACA ACAACATCAC CAGTGACAAG ATCCAGGCTG GTACTATCGA TAAACTAGGG 
AGCTTGGAGA AGCTCCTCTT CAGTCATAAC AAGCTGACTA AACCCCCTGG TTCTCTTTCG 
AAGTCTCTGG ATGAGTTGAA GTTGATCGGT AACAAGTTAA CCTCATTCCC TGCTAACACT 
TTGGCTGGGA TGGAAAACCT GACTACCGTC CACTTGTCCA AGAACAAGCT TACCACCGAG 
AGCCTCACAG GAGCCTTCAA GGGCCTCAAG TCCCTCATTC TGCTGGACGT GAGCGAGAAC 
AAGCTCAAGA AACTTCCCTC GGGAGTTCCA GCTTCGCTTT TGATGCTCTA CGCTGACAAT 
AATGACATCG ACAGCATCCC TAACGGCTAC CTGGCAAAGC TGCCACTCCT ACAGTACCTG 
CGCATCTCCC ACAACAAACT TGTAGACTCA GGGGTCCCTG CAGGAGTGTT CAATGTGTCC 
TCTCTTCTTG AGCTTGACCT GTCTTTCAAC AAGCTGAAGA CCATCCCGGA GATCAACGAA 
TCACTAGAAC ACCTCTACCT GCAAGTCAAT GAGATAAACA AGTTTGAGCT GACGAATATC 
TGCAGGTTTA GCTCACCGGT CAACTACTCT AGACTGAGGA CCCTGCGCTT GGATGGAAAC 
AACATCACAC ACAGCAGTAT GCCAGATGAC ACAGCTAACT GCCTACGCCA GGCCTCAGAG 
ATCATCTTTG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Partial depletion of yolk during zebrafish embryogenesis changes the dynamics of methionine cycle and metabolic genes.
BMC genomics16427(2015 Jun)
Huang Y,Linsen SE

Global protein expression profiling of zebrafish organs based on in vivo incorporation of stable isotopes.
Journal of proteome research13(4)2162-74(2014 Apr)
Nolte H,Konzer A,Ruhs A,Jungblut B,Braun T,Krüger M

Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung.
PloS one6(8)e24019(2011)
Zheng W,Wang Z,Collins JE,Andrews RM,Stemple D,Gong Z