upf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	upf1
Entrez Gene ID	406783
Full Name	upf1 regulator of nonsense transcripts homolog (yeast)
Synonyms	rent1,wu:fi40f07,wu:fj48a01,zgc:55472
General protein information	Preferred Names upf1 regulator of nonsense transcripts homolog (yeast) Names regulator of nonsense transcripts 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163640.4	XP_005163697.1	regulator of nonsense transcripts 1 isoform X1
XM_005163640.3	XP_005163697.1	regulator of nonsense transcripts 1 isoform X1
NM_213474.1	NP_998639.1	regulator of nonsense transcripts 1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ABR022C	NP_982968.4
Pan troglodytes (chimpanzee)	UPF1	XP_512972.4
Mus musculus (house mouse)	Upf1	NP_001116301.1
Macaca mulatta (Rhesus monkey)	LOC720288	XP_002808229.1
Canis lupus familiaris (dog)	UPF1	XP_005632737.1
Bos taurus (cattle)	UPF1	XP_005208537.1
Saccharomyces cerevisiae (baker's yeast)	NAM7	NP_013797.1
Schizosaccharomyces pombe (fission yeast)	upf1	NP_593080.1
Magnaporthe oryzae (rice blast fungus)	MGG_00976	XP_003717973.1
Oryza sativa (rice)	Os07g0495900	NP_001059698.1
Gallus gallus (chicken)	UPF1	XP_418237.3
Danio rerio (zebrafish)	upf1	NP_998639.1
Drosophila melanogaster (fruit fly)	Upf1	NP_572767.1
Kluyveromyces lactis	KLLA0B06435g	XP_451821.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001133	XP_322028.4
Homo sapiens (human)	UPF1	NP_002902.2
Caenorhabditis elegans (roundworm)	smg-2	NP_490829.1
Neurospora crassa	NCU04242	XP_961233.1
Rattus norvegicus (Norway rat)	Upf1	XP_003751614.1
Arabidopsis thaliana (thale cress)	LBA1	NP_199512.2
Xenopus tropicalis (tropical clawed frog)	upf1	XP_002938872.2

Gene Ontology
Bibliography

Related articles in PubMed

PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components.
Ma Z, Zhu P, Shi H, Guo L, Zhang Q, Chen Y, Chen S, Zhang Z, Peng J, Chen J
Nature568(7751)259-263(2019 04)

Genetic compensation triggered by mutant mRNA degradation.
El-Brolosy MA, Kontarakis Z, Rossi A, Kuenne C, Günther S, Fukuda N, Kikhi K, Boezio GLM, Takacs CM, Lai SL, Fukuda R, Gerri C, Giraldez AJ, Stainier DYR
Nature568(7751)193-197(2019 04)

Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation.
Gallagher TL, Tietz KT, Morrow ZT, McCammon JM, Goldrich ML, Derr NL, Amacher SL
Developmental biology429(1)225-239(2017 09)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following upf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the upf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02355	NM_213474.1 Latest version!	Danio rerio UPF1 RNA helicase and ATPase (upf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa07605	XM_005163640.4 Latest version!	Danio rerio upf1 regulator of nonsense transcripts homolog (yeast) (upf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa07605	XM_005163640.3	Danio rerio upf1 regulator of nonsense transcripts homolog (yeast) (upf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

upf1 ( NM_213474.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998639.1 Danio rerio UPF1 RNA helicase and ATPase (upf1), mRNA.

Price & Availability↑

CloneID	ODa02355
Clone ID Related Accession (Same CDS sequence)	NM_213474.1
Accession Version	NM_213474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Danio rerio(zebrafish)
Product	regulator of nonsense transcripts 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045353.1. ##Evidence-Data-START## Transcript exon combination :: BC045353.1, FM986817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGCTGC TCGGGGCCGA CACGCAGGGC TCCGAGTTCG AGTTTACCGA TTTTACGCTG 
CCCAGCCAGA CCCAGACGCA AGGCCAGACA CAGAGTCAGC TCGACAACCA GGTGAACGGG 
CCTGATGGAG TTCTGCCCAA TGGAGAAGAT GCAGTGGGGA AAACCAGCCA ACTTCTGGCC 
GAGCTGAACT TTGAGGAGGA CGAGGAAGAC ACTTACTACA CTAAAGTCCT GCCGGTGCAC 
GCCTGCAGCT ACTGTGGAAT CCATGATCCC GCATGTGTGG TTTACTGCAA CACCAGCAAG 
AAGTGGTTTT GCAACGGCCG CGGCAACACC TCTGGCAGTC ATATTGTGAA CCACCTGGTG 
AGGGCCAAAT GTAAGGAGGT GACGCTGCAT AAGGACGGGC CGCTGGGAGA GACTGTTCTG 
GAGTGCTACA ACTGTGGCTG CCGTAACGTC TTCCTGCTCG GCTTCATCCC GGCGAAAGCA 
GACTCTGTGG TGGTGCTTCT GTGCAGGCAG CCGTGTGCCA GTCAGAGCAG TCTGAAGGAC 
ATTAACTGGG ACAGCTCTCA ATGGCAGCCC CTCATCCAGG ACCGATGCTT TCTCTCCTGG 
CTGGTGAAGA TCCCGTCTGA GCAGGAGCAG CTGAGGGCCC GGCAGATCAC GGCGCAGCAG 
ATTAACAAAC TGGAGGAGCT CTGGAAGGAA AACCCAACAG CGACGCTGGA AGATTTGGAG 
AAGCCTGGAG TAGATGAGGA GCCGCAGCAC GTTCTGCTGC GATATGAAGA CGCCTATCAG 
TACCAGAACA TTTTTGGTCC ACTGGTTAAA CTAGAGGCCG ACTATGACAA GAAGCTCAAA 
GAGTCCCAAA CTCAAGACAA TATAACAGTG AGGTGGGATT TGGGACTGAA TAAAAAGCGG 
ATAGCTTATT TCACTCTGCC CAAGACGGAT TCAGGTGACA TGCGGCTGAT GCAAGGAGAC 
GAGATCTGTC TGCGCTATAA AGGAGACATG GCTCCGCTCT GGAAAGGCAT CGGACATGTC 
ATCAAAGTCC CGGACAATTA TGGAGATGAA ATTGCCATCG AGCTTCGTAG CAGTGCTGGT 
GCACCTGTGG AGGTGCCGCA TAACTTCCAG GTGGACTTTG TGTGGAAGTC GACATCTTTT 
GATCGAATGC AGAGCGCCCT GAAGACGTTT GCTGTGGATG AGACCTCTGT GTCAGGCTAC 
ATCTACCACA AGCTGCTGGG TCACGAGGTG GAGGACGTGA TCATCAAATG CCAGCTACCT 
AAACGCTTCA CTGCGCAGGG CCTGCCAGAC CTCAACCACT CGCAGGTTTA TGCCGTGAAG 
ACGGTACTCC AGCGGCCCCT CAGTCTGATC CAGGGGCCTC CAGGAACTGG AAAGACGGTC 
ACTTCTGCCA CCATCGTCTA TCATCTAGCG AGACAGGGCA ATGGTCCAGT GCTGGTTTGT 
GCGCCGAGTA ACATCGCTGT GGACCAGCTA ACAGAGAAGA TCCATCAGAC GGGACTGAAG 
GTGGTTCGTC TGTGTGCTAA GAGTCGTGAG GCCATCGATT CACCTGTGTC TTTCCTGGCT 
CTGCACAATC AGATCCGCAA CATGGACAGC ATGCCAGAGT TGCAGAAGCT TCAGCAGCTG 
AAGGATGAAA CTGGCGAGCT TTCGTCCTCT GATGAGAAAC GTTACCGCGC TCTCAAACGA 
ACTGCAGAGA GAGAGCTGCT CATGAATGCA GATGTGATCT GCTGCACATG TGTGGGTGCG 
GGTGACCCTC GTCTGGCTAA AATGCAGTTC CGCTCCATTC TCATCGATGA AAGCACACAG 
GCCACGGAGC CCGAGTGCAT GGTGCCAGTG GTGCTCGGGG CCAAACAGCT GATTCTGGTG 
GGTGACCACT GTCAGCTGGG CCCTGTGGTC ATGTGTAAGA AAGCAGCGAA AGCAGGCCTT 
TCTCAGTCTC TGTTCGAGAG GCTGGTGGTG CTGGGCATCA GACCCATCCG TCTGCAGGTG 
CAGTATCGCA TGCACCCGGC GCTCAGTGCT TTCCCATCAA ACATCTTCTA TGAGGGATCT 
CTGCAGAACG GGGTCACTGC CGCTGATCGC CTTAAGAAAG GCTTTGACTT CCAGTGGCCT 
CAGCCTGATA AGCCCATGTT TTTCTACGTG ACCCAGGGCC AGGAGGAGAT CGCCAGCTCC 
GGCACCTCAT ATCTCAACAG GACTGAGGCT GCTAATGTGG AGAAGATCAC CACTCGTCTG 
CTGAAGGCTG GAGCCAAACC TGACCAGATT GGCATCATCA CGCCGTATGA GGGCCAGCGC 
TCTTACCTGG TGCAGTACAT GCAGTTCAGC GGCTCATTGC ACACCAAACT CTACCAGGAG 
GTGGAGATCG CCAGTGTGGA TGCATTTCAA GGCAGAGAGA AGGACTTCAT CATCCTGTCC 
TGTGTTAGAG CTAATGAACA CCAGGGCATC GGCTTCCTGA ACGACCCGCG CCGTCTCAAC 
GTGGCACTCA CCAGAGCCAG ATATGGGGTG ATCATTGTGG GCAACCCTAA AGCTCTGTCC 
AAACAGCCCC TGTGGAACCA CCTGCTGAAC TACTACAAGG AGCAGAAGGT GCTGGTGGAG 
GGTCCTCTCA ACAACCTGAG GGAGAGTCTC ATGCAGTTCA GCAAACCACG CAAACTGGTC 
AACACCATCA ACCCTGGAGC CCGCTTCATG AGTACCGCCA TGTATGATGC AAGGGAGGCC 
ATGATTCCTG GATCTGTGTA TGATCGCAGC AGCACTGGAC GTCCATCTAA TATGTACTTC 
CAGACTCATG ATCAGGTGGG CATGATCGGG ACGGGGCCGA ATCCGATGGG CTCTTTGAAC 
ATCCCCATCC CATTCAATCT GGTCATGCCG CCGATGCCTC CGCCTGGGTA CCTGGGACAG 
GTGAACGGAC CTGCTGCAGG TCGTGGTGCT CCTAAAGGTA AGACCGGGGG TCGTGGAGGT 
CGACAGAGGA ACCGTGGCAC TGGGAATCAT GGCAGCGGGC AGCCGAACAT GCCCAACAGT 
CAGGCCAGCC AGGACCTGGT GTCTCAGCCC TTCTCTCAGG GTCCGCTGAC CCAGGGCTAT 
ATCACCATGA GCCAGCCTTC ACAGATGAGC CAGCCGGGCC TGTCCCAGCC GGAGCTCTCA 
CAGGACAGCT ACCTGGGTGA TGAGTTCAAG TCTCAGATGG ATGTTGCGCT GTCCCAGGAC 
TCCACCTACC AGGGCGAACG GGCATATCAA CACGGAGGAG TGACTGGACT CTCACAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998639.1
CDS	162..3464
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTQGQTQSQLDNQVNGPDGVLPNGEDAVGKTSQLLAELNFEEDEEDTYYTKVLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPTATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGDMRLMQGDEICLRYKGDMAPLWKGIGHVIKVPDNYGDEIAIELRSSAGAPVEVPHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSSDEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRLKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMSTAMYDAREAMIPGSVYDRSSTGRPSNMYFQTHDQVGMIGTGPNPMGSLNIPIPFNLVMPPMPPPGYLGQVNGPAAGRGAPKGKTGGRGGRQRNRGTGNHGSGQPNMPNSQASQDLVSQPFSQGPLTQGYITMSQPSQMSQPGLSQPELSQDSYLGDEFKSQMDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	NM_213474.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio UPF1 RNA helicase and ATPase (upf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213474.1

ORF Insert Sequence:

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGCTGC TCGGGGCCGA CACGCAGGGC TCCGAGTTCG AGTTTACCGA TTTTACGCTG 
CCCAGCCAGA CCCAGACGCA AGGCCAGACA CAGAGTCAGC TCGACAACCA GGTGAACGGG 
CCTGATGGAG TTCTGCCCAA TGGAGAAGAT GCAGTGGGGA AAACCAGCCA ACTTCTGGCC 
GAGCTGAACT TTGAGGAGGA CGAGGAAGAC ACTTACTACA CTAAAGTCCT GCCGGTGCAC 
GCCTGCAGCT ACTGTGGAAT CCATGATCCC GCATGTGTGG TTTACTGCAA CACCAGCAAG 
AAGTGGTTTT GCAACGGCCG CGGCAACACC TCTGGCAGTC ATATTGTGAA CCACCTGGTG 
AGGGCCAAAT GTAAGGAGGT GACGCTGCAT AAGGACGGGC CGCTGGGAGA GACTGTTCTG 
GAGTGCTACA ACTGTGGCTG CCGTAACGTC TTCCTGCTCG GCTTCATCCC GGCGAAAGCA 
GACTCTGTGG TGGTGCTTCT GTGCAGGCAG CCGTGTGCCA GTCAGAGCAG TCTGAAGGAC 
ATTAACTGGG ACAGCTCTCA ATGGCAGCCC CTCATCCAGG ACCGATGCTT TCTCTCCTGG 
CTGGTGAAGA TCCCGTCTGA GCAGGAGCAG CTGAGGGCCC GGCAGATCAC GGCGCAGCAG 
ATTAACAAAC TGGAGGAGCT CTGGAAGGAA AACCCAACAG CGACGCTGGA AGATTTGGAG 
AAGCCTGGAG TAGATGAGGA GCCGCAGCAC GTTCTGCTGC GATATGAAGA CGCCTATCAG 
TACCAGAACA TTTTTGGTCC ACTGGTTAAA CTAGAGGCCG ACTATGACAA GAAGCTCAAA 
GAGTCCCAAA CTCAAGACAA TATAACAGTG AGGTGGGATT TGGGACTGAA TAAAAAGCGG 
ATAGCTTATT TCACTCTGCC CAAGACGGAT TCAGGTGACA TGCGGCTGAT GCAAGGAGAC 
GAGATCTGTC TGCGCTATAA AGGAGACATG GCTCCGCTCT GGAAAGGCAT CGGACATGTC 
ATCAAAGTCC CGGACAATTA TGGAGATGAA ATTGCCATCG AGCTTCGTAG CAGTGCTGGT 
GCACCTGTGG AGGTGCCGCA TAACTTCCAG GTGGACTTTG TGTGGAAGTC GACATCTTTT 
GATCGAATGC AGAGCGCCCT GAAGACGTTT GCTGTGGATG AGACCTCTGT GTCAGGCTAC 
ATCTACCACA AGCTGCTGGG TCACGAGGTG GAGGACGTGA TCATCAAATG CCAGCTACCT 
AAACGCTTCA CTGCGCAGGG CCTGCCAGAC CTCAACCACT CGCAGGTTTA TGCCGTGAAG 
ACGGTACTCC AGCGGCCCCT CAGTCTGATC CAGGGGCCTC CAGGAACTGG AAAGACGGTC 
ACTTCTGCCA CCATCGTCTA TCATCTAGCG AGACAGGGCA ATGGTCCAGT GCTGGTTTGT 
GCGCCGAGTA ACATCGCTGT GGACCAGCTA ACAGAGAAGA TCCATCAGAC GGGACTGAAG 
GTGGTTCGTC TGTGTGCTAA GAGTCGTGAG GCCATCGATT CACCTGTGTC TTTCCTGGCT 
CTGCACAATC AGATCCGCAA CATGGACAGC ATGCCAGAGT TGCAGAAGCT TCAGCAGCTG 
AAGGATGAAA CTGGCGAGCT TTCGTCCTCT GATGAGAAAC GTTACCGCGC TCTCAAACGA 
ACTGCAGAGA GAGAGCTGCT CATGAATGCA GATGTGATCT GCTGCACATG TGTGGGTGCG 
GGTGACCCTC GTCTGGCTAA AATGCAGTTC CGCTCCATTC TCATCGATGA AAGCACACAG 
GCCACGGAGC CCGAGTGCAT GGTGCCAGTG GTGCTCGGGG CCAAACAGCT GATTCTGGTG 
GGTGACCACT GTCAGCTGGG CCCTGTGGTC ATGTGTAAGA AAGCAGCGAA AGCAGGCCTT 
TCTCAGTCTC TGTTCGAGAG GCTGGTGGTG CTGGGCATCA GACCCATCCG TCTGCAGGTG 
CAGTATCGCA TGCACCCGGC GCTCAGTGCT TTCCCATCAA ACATCTTCTA TGAGGGATCT 
CTGCAGAACG GGGTCACTGC CGCTGATCGC CTTAAGAAAG GCTTTGACTT CCAGTGGCCT 
CAGCCTGATA AGCCCATGTT TTTCTACGTG ACCCAGGGCC AGGAGGAGAT CGCCAGCTCC 
GGCACCTCAT ATCTCAACAG GACTGAGGCT GCTAATGTGG AGAAGATCAC CACTCGTCTG 
CTGAAGGCTG GAGCCAAACC TGACCAGATT GGCATCATCA CGCCGTATGA GGGCCAGCGC 
TCTTACCTGG TGCAGTACAT GCAGTTCAGC GGCTCATTGC ACACCAAACT CTACCAGGAG 
GTGGAGATCG CCAGTGTGGA TGCATTTCAA GGCAGAGAGA AGGACTTCAT CATCCTGTCC 
TGTGTTAGAG CTAATGAACA CCAGGGCATC GGCTTCCTGA ACGACCCGCG CCGTCTCAAC 
GTGGCACTCA CCAGAGCCAG ATATGGGGTG ATCATTGTGG GCAACCCTAA AGCTCTGTCC 
AAACAGCCCC TGTGGAACCA CCTGCTGAAC TACTACAAGG AGCAGAAGGT GCTGGTGGAG 
GGTCCTCTCA ACAACCTGAG GGAGAGTCTC ATGCAGTTCA GCAAACCACG CAAACTGGTC 
AACACCATCA ACCCTGGAGC CCGCTTCATG AGTACCGCCA TGTATGATGC AAGGGAGGCC 
ATGATTCCTG GATCTGTGTA TGATCGCAGC AGCACTGGAC GTCCATCTAA TATGTACTTC 
CAGACTCATG ATCAGGTGGG CATGATCGGG ACGGGGCCGA ATCCGATGGG CTCTTTGAAC 
ATCCCCATCC CATTCAATCT GGTCATGCCG CCGATGCCTC CGCCTGGGTA CCTGGGACAG 
GTGAACGGAC CTGCTGCAGG TCGTGGTGCT CCTAAAGGTA AGACCGGGGG TCGTGGAGGT 
CGACAGAGGA ACCGTGGCAC TGGGAATCAT GGCAGCGGGC AGCCGAACAT GCCCAACAGT 
CAGGCCAGCC AGGACCTGGT GTCTCAGCCC TTCTCTCAGG GTCCGCTGAC CCAGGGCTAT 
ATCACCATGA GCCAGCCTTC ACAGATGAGC CAGCCGGGCC TGTCCCAGCC GGAGCTCTCA 
CAGGACAGCT ACCTGGGTGA TGAGTTCAAG TCTCAGATGG ATGTTGCGCT GTCCCAGGAC 
TCCACCTACC AGGGCGAACG GGCATATCAA CACGGAGGAG TGACTGGACT CTCACAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upf1 ( XM_005163640.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163697.1 Danio rerio upf1 regulator of nonsense transcripts homolog (yeast) (upf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07605
Clone ID Related Accession (Same CDS sequence)	XM_005163640.4 , XM_005163640.3
Accession Version	XM_005163640.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163640.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGCTGC TCGGGGCCGA CACGCAGGGC TCCGAGTTCG AGTTTACCGA TTTTACGCTG 
CCCAGCCAGA CCCAGACGCA AGGCCAGACA CAGAGTCAGC TCGACAACCA GGTGAACGGG 
CCTGATGGAG TTCTGCCCAA TGGAGAAGAT GCAGTGGGGA AAACCAGCCA ACTTCTGGCC 
GAGCTGAACT TTGAGGAGGA CGAGGAAGAC ACTTACTACA CTAAAGACCT GCCGGTGCAC 
GCCTGCAGCT ACTGTGGAAT CCATGATCCC GCATGTGTGG TTTACTGCAA CACCAGCAAG 
AAGTGGTTTT GCAACGGCCG CGGCAACACC TCTGGCAGTC ATATTGTGAA CCACCTGGTG 
AGGGCCAAAT GTAAGGAGGT GACGCTGCAT AAGGACGGGC CGCTGGGAGA GACTGTTCTG 
GAGTGCTACA ACTGTGGCTG CCGTAACGTC TTCCTGCTCG GCTTCATCCC TGCGAAAGCA 
GACTCTGTGG TGGTGCTTCT GTGCAGGCAG CCGTGTGCCA GTCAGAGCAG TCTGAAGGAC 
ATTAACTGGG ACAGCTCTCA ATGGCAGCCC CTCATCCAGG ACCGATGCTT TCTCTCCTGG 
CTGGTGAAGA TCCCGTCTGA GCAGGAGCAG CTGAGGGCCC GGCAGATCAC GGCGCAGCAG 
ATTAACAAAC TGGAGGAGCT CTGGAAGGAA AACCCAACAG CGACGCTGGA AGATCTGGAG 
AAGCCTGGAG TAGATGAGGA GCCGCAGCAC GTTCTGCTAC GATATGAAGA CGCCTATCAG 
TACCAGAACA TTTTTGGTCC ACTGGTTAAA CTAGAGGCCG ACTATGACAA GAAGCTCAAA 
GAGTCCCAAA CTCAAGACAA TATAACAGTG AGGTGGGATT TGGGACTGAA TAAAAAGCGG 
ATAGCTTATT TCACTCTGCC CAAGACGGAT TCAGACATGC GGCTGATGCA AGGAGACGAG 
ATCTGTCTGC GCTATAAAGG AGACATGGCT CCGCTCTGGA AAGGCATCGG ACATGTCATC 
AAAGTCCCGG ACAATTATGG AGATGAAATT GCCATCGAGC TGCGCAGCAG TGCTGGTGCA 
CCTGTGGAGG TGCCACATAA CTTCCAGGTG GACTTTGTGT GGAAGTCGAC ATCTTTTGAT 
CGAATGCAGA GCGCCCTGAA GACGTTTGCT GTGGATGAGA CCTCTGTGTC AGGCTACATC 
TACCACAAGC TGCTGGGTCA CGAGGTGGAG GACGTGATCA TCAAATGCCA GCTACCTAAA 
CGCTTCACTG CGCAGGGCCT GCCAGACCTC AACCACTCGC AGGTTTATGC CGTGAAGACG 
GTACTCCAGC GGCCCCTCAG TCTGATCCAG GGGCCTCCAG GAACTGGAAA GACGGTCACT 
TCTGCCACCA TCGTCTATCA TCTAGCGAGA CAGGGCAATG GTCCAGTGCT GGTTTGTGCG 
CCGAGTAACA TCGCTGTGGA CCAGCTAACA GAGAAGATCC ATCAGACGGG ACTGAAGGTG 
GTTCGTCTGT GTGCTAAGAG TCGTGAGGCC ATCGATTCAC CTGTGTCTTT CCTGGCTCTG 
CACAATCAGA TCCGCAACAT GGACAGCATG CCAGAGTTGC AGAAGCTTCA GCAGCTGAAG 
GATGAAACTG GCGAGCTTTC GTCCTCTGAT GAGAAACGTT ACCGTGCTCT CAAACGAACT 
GCAGAGAGAG AGCTGCTCAT GAATGCAGAT GTGATCTGCT GCACATGTGT GGGTGCGGGT 
GACCCTCGTC TGGCTAAAAT GCAGTTCCGC TCCATTCTCA TCGATGAAAG CACACAGGCC 
ACGGAGCCCG AGTGCATGGT GCCAGTGGTG CTCGGGGCTA AACAGCTGAT TCTGGTCGGT 
GACCACTGTC AGCTGGGCCC TGTGGTCATG TGTAAGAAAG CAGCGAAAGC AGGCCTTTCT 
CAGTCTCTGT TCGAGAGGCT GGTGGTGCTG GGCATCAGAC CCATCCGTCT GCAGGTGCAG 
TACCGCATGC ACCCGGCGCT CAGTGCTTTC CCATCAAACA TCTTCTATGA GGGATCTCTG 
CAGAACGGGG TCACTGCCGC TGATCGCCTT AAGAAAGGCT TTGACTTCCA GTGGCCCCAG 
CCTGATAAGC CCATGTTTTT CTACGTGACC CAGGGCCAGG AGGAGATCGC CAGCTCCGGC 
ACCTCATATC TCAACAGGAC TGAGGCTGCT AATGTGGAGA AGATCACCAC TCGTCTGCTG 
AAGGCCGGAG CCAAACCTGA CCAGATTGGC ATCATCACGC CGTATGAGGG CCAGCGCTCT 
TACCTGGTGC AGTACATGCA GTTCAGCGGC TCATTGCACA CCAAACTCTA CCAGGAGGTG 
GAGATCGCCA GTGTGGATGC ATTTCAAGGC AGAGAGAAGG ACTTCATCAT CCTGTCCTGT 
GTCAGAGCTA ATGAACACCA GGGCATCGGC TTCCTGAACG ACCCGCGCCG TCTCAATGTG 
GCACTCACCA GAGCCAGATA TGGGGTGATC ATTGTGGGCA ACCCTAAAGC TCTGTCCAAA 
CAGCCCCTGT GGAACCACCT GCTGAACTAC TACAAGGAGC AGAAGGTGCT GGTGGAGGGT 
CCTCTCAACA ACCTGAGGGA GAGTCTCATG CAGTTCAGCA AACCACGCAA ACTGGTCAAC 
ACCATCAACC CTGGAGCCCG CTTCATGAGT ACCGCCATGT ATGATGCAAG GGAGGCCATG 
ATTCCTGGAT CTGTGTATGA TCGCAGCAGC ACTGGACGTC CATCTAATAT GTACTTCCAG 
ACTCATGATC AGGTGGGCAT GATCGGGACG GGCCCAAATC CGATGGGCTC TTTGAACATC 
CCCATCCCAT TCAATCTGGT CATGCCGCCG ATGCCTCCGC CTGGGTATCT GGGACAGGTG 
AACGGACCTG CTGCAGGTCG TGGTGCTCCT AAAGGTAAGA CCGGGGGTCG TGGAGGTCGA 
CAGAGGAACC GTGGCACTGG GAATCATGGC AGCGGGCAGC CGAACATGCC CAACAGTCAG 
GCCAGCCAGG ACCTGGTGTC TCAGCCCTTC TCTCAGGGTC CGCTGACCCA GGGCTATATC 
ACCATGAGCC AGCCTTCACA GATGAGCCAG CCGGGCCTGT CCCAGCCGGA GCTCTCACAG 
GACAGCTACC TGGGTGATGA GTTCAAGTCT CAGATGGATG TTGCGCTGTC CCAGGACTCC 
ACCTACCAGG GCGAACGGGC ATATCAACAC GGAGGAGTGA CTGGACTCTC ACAGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163697.1
CDS	209..3508
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTQGQTQSQLDNQVNGPDGVLPNGEDAVGKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPTATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDMAPLWKGIGHVIKVPDNYGDEIAIELRSSAGAPVEVPHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSSDEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRLKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMSTAMYDAREAMIPGSVYDRSSTGRPSNMYFQTHDQVGMIGTGPNPMGSLNIPIPFNLVMPPMPPPGYLGQVNGPAAGRGAPKGKTGGRGGRQRNRGTGNHGSGQPNMPNSQASQDLVSQPFSQGPLTQGYITMSQPSQMSQPGLSQPELSQDSYLGDEFKSQMDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_005163640.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio upf1 regulator of nonsense transcripts homolog (yeast) (upf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163640.4

ORF Insert Sequence:

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGCTGC TCGGGGCCGA CACGCAGGGC TCCGAGTTCG AGTTTACCGA TTTTACGCTG 
CCCAGCCAGA CCCAGACGCA AGGCCAGACA CAGAGTCAGC TCGACAACCA GGTGAACGGG 
CCTGATGGAG TTCTGCCCAA TGGAGAAGAT GCAGTGGGGA AAACCAGCCA ACTTCTGGCC 
GAGCTGAACT TTGAGGAGGA CGAGGAAGAC ACTTACTACA CTAAAGACCT GCCGGTGCAC 
GCCTGCAGCT ACTGTGGAAT CCATGATCCC GCATGTGTGG TTTACTGCAA CACCAGCAAG 
AAGTGGTTTT GCAACGGCCG CGGCAACACC TCTGGCAGTC ATATTGTGAA CCACCTGGTG 
AGGGCCAAAT GTAAGGAGGT GACGCTGCAT AAGGACGGGC CGCTGGGAGA GACTGTTCTG 
GAGTGCTACA ACTGTGGCTG CCGTAACGTC TTCCTGCTCG GCTTCATCCC TGCGAAAGCA 
GACTCTGTGG TGGTGCTTCT GTGCAGGCAG CCGTGTGCCA GTCAGAGCAG TCTGAAGGAC 
ATTAACTGGG ACAGCTCTCA ATGGCAGCCC CTCATCCAGG ACCGATGCTT TCTCTCCTGG 
CTGGTGAAGA TCCCGTCTGA GCAGGAGCAG CTGAGGGCCC GGCAGATCAC GGCGCAGCAG 
ATTAACAAAC TGGAGGAGCT CTGGAAGGAA AACCCAACAG CGACGCTGGA AGATCTGGAG 
AAGCCTGGAG TAGATGAGGA GCCGCAGCAC GTTCTGCTAC GATATGAAGA CGCCTATCAG 
TACCAGAACA TTTTTGGTCC ACTGGTTAAA CTAGAGGCCG ACTATGACAA GAAGCTCAAA 
GAGTCCCAAA CTCAAGACAA TATAACAGTG AGGTGGGATT TGGGACTGAA TAAAAAGCGG 
ATAGCTTATT TCACTCTGCC CAAGACGGAT TCAGACATGC GGCTGATGCA AGGAGACGAG 
ATCTGTCTGC GCTATAAAGG AGACATGGCT CCGCTCTGGA AAGGCATCGG ACATGTCATC 
AAAGTCCCGG ACAATTATGG AGATGAAATT GCCATCGAGC TGCGCAGCAG TGCTGGTGCA 
CCTGTGGAGG TGCCACATAA CTTCCAGGTG GACTTTGTGT GGAAGTCGAC ATCTTTTGAT 
CGAATGCAGA GCGCCCTGAA GACGTTTGCT GTGGATGAGA CCTCTGTGTC AGGCTACATC 
TACCACAAGC TGCTGGGTCA CGAGGTGGAG GACGTGATCA TCAAATGCCA GCTACCTAAA 
CGCTTCACTG CGCAGGGCCT GCCAGACCTC AACCACTCGC AGGTTTATGC CGTGAAGACG 
GTACTCCAGC GGCCCCTCAG TCTGATCCAG GGGCCTCCAG GAACTGGAAA GACGGTCACT 
TCTGCCACCA TCGTCTATCA TCTAGCGAGA CAGGGCAATG GTCCAGTGCT GGTTTGTGCG 
CCGAGTAACA TCGCTGTGGA CCAGCTAACA GAGAAGATCC ATCAGACGGG ACTGAAGGTG 
GTTCGTCTGT GTGCTAAGAG TCGTGAGGCC ATCGATTCAC CTGTGTCTTT CCTGGCTCTG 
CACAATCAGA TCCGCAACAT GGACAGCATG CCAGAGTTGC AGAAGCTTCA GCAGCTGAAG 
GATGAAACTG GCGAGCTTTC GTCCTCTGAT GAGAAACGTT ACCGTGCTCT CAAACGAACT 
GCAGAGAGAG AGCTGCTCAT GAATGCAGAT GTGATCTGCT GCACATGTGT GGGTGCGGGT 
GACCCTCGTC TGGCTAAAAT GCAGTTCCGC TCCATTCTCA TCGATGAAAG CACACAGGCC 
ACGGAGCCCG AGTGCATGGT GCCAGTGGTG CTCGGGGCTA AACAGCTGAT TCTGGTCGGT 
GACCACTGTC AGCTGGGCCC TGTGGTCATG TGTAAGAAAG CAGCGAAAGC AGGCCTTTCT 
CAGTCTCTGT TCGAGAGGCT GGTGGTGCTG GGCATCAGAC CCATCCGTCT GCAGGTGCAG 
TACCGCATGC ACCCGGCGCT CAGTGCTTTC CCATCAAACA TCTTCTATGA GGGATCTCTG 
CAGAACGGGG TCACTGCCGC TGATCGCCTT AAGAAAGGCT TTGACTTCCA GTGGCCCCAG 
CCTGATAAGC CCATGTTTTT CTACGTGACC CAGGGCCAGG AGGAGATCGC CAGCTCCGGC 
ACCTCATATC TCAACAGGAC TGAGGCTGCT AATGTGGAGA AGATCACCAC TCGTCTGCTG 
AAGGCCGGAG CCAAACCTGA CCAGATTGGC ATCATCACGC CGTATGAGGG CCAGCGCTCT 
TACCTGGTGC AGTACATGCA GTTCAGCGGC TCATTGCACA CCAAACTCTA CCAGGAGGTG 
GAGATCGCCA GTGTGGATGC ATTTCAAGGC AGAGAGAAGG ACTTCATCAT CCTGTCCTGT 
GTCAGAGCTA ATGAACACCA GGGCATCGGC TTCCTGAACG ACCCGCGCCG TCTCAATGTG 
GCACTCACCA GAGCCAGATA TGGGGTGATC ATTGTGGGCA ACCCTAAAGC TCTGTCCAAA 
CAGCCCCTGT GGAACCACCT GCTGAACTAC TACAAGGAGC AGAAGGTGCT GGTGGAGGGT 
CCTCTCAACA ACCTGAGGGA GAGTCTCATG CAGTTCAGCA AACCACGCAA ACTGGTCAAC 
ACCATCAACC CTGGAGCCCG CTTCATGAGT ACCGCCATGT ATGATGCAAG GGAGGCCATG 
ATTCCTGGAT CTGTGTATGA TCGCAGCAGC ACTGGACGTC CATCTAATAT GTACTTCCAG 
ACTCATGATC AGGTGGGCAT GATCGGGACG GGCCCAAATC CGATGGGCTC TTTGAACATC 
CCCATCCCAT TCAATCTGGT CATGCCGCCG ATGCCTCCGC CTGGGTATCT GGGACAGGTG 
AACGGACCTG CTGCAGGTCG TGGTGCTCCT AAAGGTAAGA CCGGGGGTCG TGGAGGTCGA 
CAGAGGAACC GTGGCACTGG GAATCATGGC AGCGGGCAGC CGAACATGCC CAACAGTCAG 
GCCAGCCAGG ACCTGGTGTC TCAGCCCTTC TCTCAGGGTC CGCTGACCCA GGGCTATATC 
ACCATGAGCC AGCCTTCACA GATGAGCCAG CCGGGCCTGT CCCAGCCGGA GCTCTCACAG 
GACAGCTACC TGGGTGATGA GTTCAAGTCT CAGATGGATG TTGCGCTGTC CCAGGACTCC 
ACCTACCAGG GCGAACGGGC ATATCAACAC GGAGGAGTGA CTGGACTCTC ACAGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upf1 ( XM_005163640.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163697.1 Danio rerio upf1 regulator of nonsense transcripts homolog (yeast) (upf1), transcript variant X1, mRNA.

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CloneID	ODa07605
Clone ID Related Accession (Same CDS sequence)	XM_005163640.4 , XM_005163640.3
Accession Version	XM_005163640.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163640.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGCTGC TCGGGGCCGA CACGCAGGGC TCCGAGTTCG AGTTTACCGA TTTTACGCTG 
CCCAGCCAGA CCCAGACGCA AGGCCAGACA CAGAGTCAGC TCGACAACCA GGTGAACGGG 
CCTGATGGAG TTCTGCCCAA TGGAGAAGAT GCAGTGGGGA AAACCAGCCA ACTTCTGGCC 
GAGCTGAACT TTGAGGAGGA CGAGGAAGAC ACTTACTACA CTAAAGACCT GCCGGTGCAC 
GCCTGCAGCT ACTGTGGAAT CCATGATCCC GCATGTGTGG TTTACTGCAA CACCAGCAAG 
AAGTGGTTTT GCAACGGCCG CGGCAACACC TCTGGCAGTC ATATTGTGAA CCACCTGGTG 
AGGGCCAAAT GTAAGGAGGT GACGCTGCAT AAGGACGGGC CGCTGGGAGA GACTGTTCTG 
GAGTGCTACA ACTGTGGCTG CCGTAACGTC TTCCTGCTCG GCTTCATCCC TGCGAAAGCA 
GACTCTGTGG TGGTGCTTCT GTGCAGGCAG CCGTGTGCCA GTCAGAGCAG TCTGAAGGAC 
ATTAACTGGG ACAGCTCTCA ATGGCAGCCC CTCATCCAGG ACCGATGCTT TCTCTCCTGG 
CTGGTGAAGA TCCCGTCTGA GCAGGAGCAG CTGAGGGCCC GGCAGATCAC GGCGCAGCAG 
ATTAACAAAC TGGAGGAGCT CTGGAAGGAA AACCCAACAG CGACGCTGGA AGATCTGGAG 
AAGCCTGGAG TAGATGAGGA GCCGCAGCAC GTTCTGCTAC GATATGAAGA CGCCTATCAG 
TACCAGAACA TTTTTGGTCC ACTGGTTAAA CTAGAGGCCG ACTATGACAA GAAGCTCAAA 
GAGTCCCAAA CTCAAGACAA TATAACAGTG AGGTGGGATT TGGGACTGAA TAAAAAGCGG 
ATAGCTTATT TCACTCTGCC CAAGACGGAT TCAGACATGC GGCTGATGCA AGGAGACGAG 
ATCTGTCTGC GCTATAAAGG AGACATGGCT CCGCTCTGGA AAGGCATCGG ACATGTCATC 
AAAGTCCCGG ACAATTATGG AGATGAAATT GCCATCGAGC TGCGCAGCAG TGCTGGTGCA 
CCTGTGGAGG TGCCACATAA CTTCCAGGTG GACTTTGTGT GGAAGTCGAC ATCTTTTGAT 
CGAATGCAGA GCGCCCTGAA GACGTTTGCT GTGGATGAGA CCTCTGTGTC AGGCTACATC 
TACCACAAGC TGCTGGGTCA CGAGGTGGAG GACGTGATCA TCAAATGCCA GCTACCTAAA 
CGCTTCACTG CGCAGGGCCT GCCAGACCTC AACCACTCGC AGGTTTATGC CGTGAAGACG 
GTACTCCAGC GGCCCCTCAG TCTGATCCAG GGGCCTCCAG GAACTGGAAA GACGGTCACT 
TCTGCCACCA TCGTCTATCA TCTAGCGAGA CAGGGCAATG GTCCAGTGCT GGTTTGTGCG 
CCGAGTAACA TCGCTGTGGA CCAGCTAACA GAGAAGATCC ATCAGACGGG ACTGAAGGTG 
GTTCGTCTGT GTGCTAAGAG TCGTGAGGCC ATCGATTCAC CTGTGTCTTT CCTGGCTCTG 
CACAATCAGA TCCGCAACAT GGACAGCATG CCAGAGTTGC AGAAGCTTCA GCAGCTGAAG 
GATGAAACTG GCGAGCTTTC GTCCTCTGAT GAGAAACGTT ACCGTGCTCT CAAACGAACT 
GCAGAGAGAG AGCTGCTCAT GAATGCAGAT GTGATCTGCT GCACATGTGT GGGTGCGGGT 
GACCCTCGTC TGGCTAAAAT GCAGTTCCGC TCCATTCTCA TCGATGAAAG CACACAGGCC 
ACGGAGCCCG AGTGCATGGT GCCAGTGGTG CTCGGGGCTA AACAGCTGAT TCTGGTCGGT 
GACCACTGTC AGCTGGGCCC TGTGGTCATG TGTAAGAAAG CAGCGAAAGC AGGCCTTTCT 
CAGTCTCTGT TCGAGAGGCT GGTGGTGCTG GGCATCAGAC CCATCCGTCT GCAGGTGCAG 
TACCGCATGC ACCCGGCGCT CAGTGCTTTC CCATCAAACA TCTTCTATGA GGGATCTCTG 
CAGAACGGGG TCACTGCCGC TGATCGCCTT AAGAAAGGCT TTGACTTCCA GTGGCCCCAG 
CCTGATAAGC CCATGTTTTT CTACGTGACC CAGGGCCAGG AGGAGATCGC CAGCTCCGGC 
ACCTCATATC TCAACAGGAC TGAGGCTGCT AATGTGGAGA AGATCACCAC TCGTCTGCTG 
AAGGCCGGAG CCAAACCTGA CCAGATTGGC ATCATCACGC CGTATGAGGG CCAGCGCTCT 
TACCTGGTGC AGTACATGCA GTTCAGCGGC TCATTGCACA CCAAACTCTA CCAGGAGGTG 
GAGATCGCCA GTGTGGATGC ATTTCAAGGC AGAGAGAAGG ACTTCATCAT CCTGTCCTGT 
GTCAGAGCTA ATGAACACCA GGGCATCGGC TTCCTGAACG ACCCGCGCCG TCTCAATGTG 
GCACTCACCA GAGCCAGATA TGGGGTGATC ATTGTGGGCA ACCCTAAAGC TCTGTCCAAA 
CAGCCCCTGT GGAACCACCT GCTGAACTAC TACAAGGAGC AGAAGGTGCT GGTGGAGGGT 
CCTCTCAACA ACCTGAGGGA GAGTCTCATG CAGTTCAGCA AACCACGCAA ACTGGTCAAC 
ACCATCAACC CTGGAGCCCG CTTCATGAGT ACCGCCATGT ATGATGCAAG GGAGGCCATG 
ATTCCTGGAT CTGTGTATGA TCGCAGCAGC ACTGGACGTC CATCTAATAT GTACTTCCAG 
ACTCATGATC AGGTGGGCAT GATCGGGACG GGCCCAAATC CGATGGGCTC TTTGAACATC 
CCCATCCCAT TCAATCTGGT CATGCCGCCG ATGCCTCCGC CTGGGTATCT GGGACAGGTG 
AACGGACCTG CTGCAGGTCG TGGTGCTCCT AAAGGTAAGA CCGGGGGTCG TGGAGGTCGA 
CAGAGGAACC GTGGCACTGG GAATCATGGC AGCGGGCAGC CGAACATGCC CAACAGTCAG 
GCCAGCCAGG ACCTGGTGTC TCAGCCCTTC TCTCAGGGTC CGCTGACCCA GGGCTATATC 
ACCATGAGCC AGCCTTCACA GATGAGCCAG CCGGGCCTGT CCCAGCCGGA GCTCTCACAG 
GACAGCTACC TGGGTGATGA GTTCAAGTCT CAGATGGATG TTGCGCTGTC CCAGGACTCC 
ACCTACCAGG GCGAACGGGC ATATCAACAC GGAGGAGTGA CTGGACTCTC ACAGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163697.1
CDS	264..3563
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTQGQTQSQLDNQVNGPDGVLPNGEDAVGKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPTATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDMAPLWKGIGHVIKVPDNYGDEIAIELRSSAGAPVEVPHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSSDEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRLKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMSTAMYDAREAMIPGSVYDRSSTGRPSNMYFQTHDQVGMIGTGPNPMGSLNIPIPFNLVMPPMPPPGYLGQVNGPAAGRGAPKGKTGGRGGRQRNRGTGNHGSGQPNMPNSQASQDLVSQPFSQGPLTQGYITMSQPSQMSQPGLSQPELSQDSYLGDEFKSQMDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_005163640.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio upf1 regulator of nonsense transcripts homolog (yeast) (upf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163640.3

ORF Insert Sequence:

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGCTGC TCGGGGCCGA CACGCAGGGC TCCGAGTTCG AGTTTACCGA TTTTACGCTG 
CCCAGCCAGA CCCAGACGCA AGGCCAGACA CAGAGTCAGC TCGACAACCA GGTGAACGGG 
CCTGATGGAG TTCTGCCCAA TGGAGAAGAT GCAGTGGGGA AAACCAGCCA ACTTCTGGCC 
GAGCTGAACT TTGAGGAGGA CGAGGAAGAC ACTTACTACA CTAAAGACCT GCCGGTGCAC 
GCCTGCAGCT ACTGTGGAAT CCATGATCCC GCATGTGTGG TTTACTGCAA CACCAGCAAG 
AAGTGGTTTT GCAACGGCCG CGGCAACACC TCTGGCAGTC ATATTGTGAA CCACCTGGTG 
AGGGCCAAAT GTAAGGAGGT GACGCTGCAT AAGGACGGGC CGCTGGGAGA GACTGTTCTG 
GAGTGCTACA ACTGTGGCTG CCGTAACGTC TTCCTGCTCG GCTTCATCCC TGCGAAAGCA 
GACTCTGTGG TGGTGCTTCT GTGCAGGCAG CCGTGTGCCA GTCAGAGCAG TCTGAAGGAC 
ATTAACTGGG ACAGCTCTCA ATGGCAGCCC CTCATCCAGG ACCGATGCTT TCTCTCCTGG 
CTGGTGAAGA TCCCGTCTGA GCAGGAGCAG CTGAGGGCCC GGCAGATCAC GGCGCAGCAG 
ATTAACAAAC TGGAGGAGCT CTGGAAGGAA AACCCAACAG CGACGCTGGA AGATCTGGAG 
AAGCCTGGAG TAGATGAGGA GCCGCAGCAC GTTCTGCTAC GATATGAAGA CGCCTATCAG 
TACCAGAACA TTTTTGGTCC ACTGGTTAAA CTAGAGGCCG ACTATGACAA GAAGCTCAAA 
GAGTCCCAAA CTCAAGACAA TATAACAGTG AGGTGGGATT TGGGACTGAA TAAAAAGCGG 
ATAGCTTATT TCACTCTGCC CAAGACGGAT TCAGACATGC GGCTGATGCA AGGAGACGAG 
ATCTGTCTGC GCTATAAAGG AGACATGGCT CCGCTCTGGA AAGGCATCGG ACATGTCATC 
AAAGTCCCGG ACAATTATGG AGATGAAATT GCCATCGAGC TGCGCAGCAG TGCTGGTGCA 
CCTGTGGAGG TGCCACATAA CTTCCAGGTG GACTTTGTGT GGAAGTCGAC ATCTTTTGAT 
CGAATGCAGA GCGCCCTGAA GACGTTTGCT GTGGATGAGA CCTCTGTGTC AGGCTACATC 
TACCACAAGC TGCTGGGTCA CGAGGTGGAG GACGTGATCA TCAAATGCCA GCTACCTAAA 
CGCTTCACTG CGCAGGGCCT GCCAGACCTC AACCACTCGC AGGTTTATGC CGTGAAGACG 
GTACTCCAGC GGCCCCTCAG TCTGATCCAG GGGCCTCCAG GAACTGGAAA GACGGTCACT 
TCTGCCACCA TCGTCTATCA TCTAGCGAGA CAGGGCAATG GTCCAGTGCT GGTTTGTGCG 
CCGAGTAACA TCGCTGTGGA CCAGCTAACA GAGAAGATCC ATCAGACGGG ACTGAAGGTG 
GTTCGTCTGT GTGCTAAGAG TCGTGAGGCC ATCGATTCAC CTGTGTCTTT CCTGGCTCTG 
CACAATCAGA TCCGCAACAT GGACAGCATG CCAGAGTTGC AGAAGCTTCA GCAGCTGAAG 
GATGAAACTG GCGAGCTTTC GTCCTCTGAT GAGAAACGTT ACCGTGCTCT CAAACGAACT 
GCAGAGAGAG AGCTGCTCAT GAATGCAGAT GTGATCTGCT GCACATGTGT GGGTGCGGGT 
GACCCTCGTC TGGCTAAAAT GCAGTTCCGC TCCATTCTCA TCGATGAAAG CACACAGGCC 
ACGGAGCCCG AGTGCATGGT GCCAGTGGTG CTCGGGGCTA AACAGCTGAT TCTGGTCGGT 
GACCACTGTC AGCTGGGCCC TGTGGTCATG TGTAAGAAAG CAGCGAAAGC AGGCCTTTCT 
CAGTCTCTGT TCGAGAGGCT GGTGGTGCTG GGCATCAGAC CCATCCGTCT GCAGGTGCAG 
TACCGCATGC ACCCGGCGCT CAGTGCTTTC CCATCAAACA TCTTCTATGA GGGATCTCTG 
CAGAACGGGG TCACTGCCGC TGATCGCCTT AAGAAAGGCT TTGACTTCCA GTGGCCCCAG 
CCTGATAAGC CCATGTTTTT CTACGTGACC CAGGGCCAGG AGGAGATCGC CAGCTCCGGC 
ACCTCATATC TCAACAGGAC TGAGGCTGCT AATGTGGAGA AGATCACCAC TCGTCTGCTG 
AAGGCCGGAG CCAAACCTGA CCAGATTGGC ATCATCACGC CGTATGAGGG CCAGCGCTCT 
TACCTGGTGC AGTACATGCA GTTCAGCGGC TCATTGCACA CCAAACTCTA CCAGGAGGTG 
GAGATCGCCA GTGTGGATGC ATTTCAAGGC AGAGAGAAGG ACTTCATCAT CCTGTCCTGT 
GTCAGAGCTA ATGAACACCA GGGCATCGGC TTCCTGAACG ACCCGCGCCG TCTCAATGTG 
GCACTCACCA GAGCCAGATA TGGGGTGATC ATTGTGGGCA ACCCTAAAGC TCTGTCCAAA 
CAGCCCCTGT GGAACCACCT GCTGAACTAC TACAAGGAGC AGAAGGTGCT GGTGGAGGGT 
CCTCTCAACA ACCTGAGGGA GAGTCTCATG CAGTTCAGCA AACCACGCAA ACTGGTCAAC 
ACCATCAACC CTGGAGCCCG CTTCATGAGT ACCGCCATGT ATGATGCAAG GGAGGCCATG 
ATTCCTGGAT CTGTGTATGA TCGCAGCAGC ACTGGACGTC CATCTAATAT GTACTTCCAG 
ACTCATGATC AGGTGGGCAT GATCGGGACG GGCCCAAATC CGATGGGCTC TTTGAACATC 
CCCATCCCAT TCAATCTGGT CATGCCGCCG ATGCCTCCGC CTGGGTATCT GGGACAGGTG 
AACGGACCTG CTGCAGGTCG TGGTGCTCCT AAAGGTAAGA CCGGGGGTCG TGGAGGTCGA 
CAGAGGAACC GTGGCACTGG GAATCATGGC AGCGGGCAGC CGAACATGCC CAACAGTCAG 
GCCAGCCAGG ACCTGGTGTC TCAGCCCTTC TCTCAGGGTC CGCTGACCCA GGGCTATATC 
ACCATGAGCC AGCCTTCACA GATGAGCCAG CCGGGCCTGT CCCAGCCGGA GCTCTCACAG 
GACAGCTACC TGGGTGATGA GTTCAAGTCT CAGATGGATG TTGCGCTGTC CCAGGACTCC 
ACCTACCAGG GCGAACGGGC ATATCAACAC GGAGGAGTGA CTGGACTCTC ACAGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components.
Nature568(7751)259-263(2019 04)
Ma Z,Zhu P,Shi H,Guo L,Zhang Q,Chen Y,Chen S,Zhang Z,Peng J,Chen J

Genetic compensation triggered by mutant mRNA degradation.
Nature568(7751)193-197(2019 04)
El-Brolosy MA,Kontarakis Z,Rossi A,Kuenne C,Günther S,Fukuda N,Kikhi K,Boezio GLM,Takacs CM,Lai SL,Fukuda R,Gerri C,Giraldez AJ,Stainier DYR

Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation.
Developmental biology429(1)225-239(2017 09)
Gallagher TL,Tietz KT,Morrow ZT,McCammon JM,Goldrich ML,Derr NL,Amacher SL

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

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upf1 cDNA ORF clone, Danio rerio(zebrafish)

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