UPF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	UPF1
Entrez Gene ID	476664
Full Name	UPF1, RNA helicase and ATPase
General protein information	Preferred Names UPF1, RNA helicase and ATPase Names regulator of nonsense transcripts 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_533868.5	XP_533868.2	regulator of nonsense transcripts 1 isoform X4
XM_022406838.1	XP_022262546.1	regulator of nonsense transcripts 1 isoform X3
XM_022406837.1	XP_022262545.1	regulator of nonsense transcripts 1 isoform X1
XM_533868.4	XP_533868.2	regulator of nonsense transcripts 1 isoform X2
XM_005632680.2	XP_005632737.1	regulator of nonsense transcripts 1 isoform X1
XM_005632680.3	XP_005632737.1	regulator of nonsense transcripts 1 isoform X2

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ABR022C	NP_982968.4
Pan troglodytes (chimpanzee)	UPF1	XP_512972.4
Mus musculus (house mouse)	Upf1	NP_001116301.1
Macaca mulatta (Rhesus monkey)	LOC720288	XP_002808229.1
Canis lupus familiaris (dog)	UPF1	XP_005632737.1
Bos taurus (cattle)	UPF1	XP_005208537.1
Saccharomyces cerevisiae (baker's yeast)	NAM7	NP_013797.1
Schizosaccharomyces pombe (fission yeast)	upf1	NP_593080.1
Magnaporthe oryzae (rice blast fungus)	MGG_00976	XP_003717973.1
Oryza sativa (rice)	Os07g0495900	NP_001059698.1
Gallus gallus (chicken)	UPF1	XP_418237.3
Danio rerio (zebrafish)	upf1	NP_998639.1
Drosophila melanogaster (fruit fly)	Upf1	NP_572767.1
Kluyveromyces lactis	KLLA0B06435g	XP_451821.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001133	XP_322028.4
Homo sapiens (human)	UPF1	NP_002902.2
Caenorhabditis elegans (roundworm)	smg-2	NP_490829.1
Neurospora crassa	NCU04242	XP_961233.1
Rattus norvegicus (Norway rat)	Upf1	XP_003751614.1
Arabidopsis thaliana (thale cress)	LBA1	NP_199512.2
Xenopus tropicalis (tropical clawed frog)	upf1	XP_002938872.2

Gene Ontology

The following UPF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UPF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe21853	XM_005632680.3 Latest version!	Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe21854	XM_533868.5 Latest version!	Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe295500	XM_022406837.1 Latest version!	Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe295501	XM_022406838.1 Latest version!	Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe21854	XM_533868.4	Canis lupus familiaris UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe21853	XM_005632680.2	Canis lupus familiaris UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UPF1 ( XM_005632680.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632737.1 Canis lupus familiaris UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe21853
Clone ID Related Accession (Same CDS sequence)	XM_005632680.2 , XM_005632680.3
Accession Version	XM_005632680.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005632680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG GTAATGAGGA TTTAGTCATA 
ATTTGGTTAA GAGACATGCG GCTCATGCAG GGGGATGAGA TATGCCTGAG GTACAAAGGG 
GATCTGGCAC CCCTCTGGAA AGGGATCGGC CACGTCATCA AGGTCCCTGA TAATTATGGC 
GATGAGATCG CCATTGAGCT TCGGAGCAGT GTGGGTGCAC CTGTGGAGGT GACTCATAAC 
TTCCAGGTGG ATTTCGTATG GAAGTCAACC TCCTTTGATA GGATGCAGAG TGCGTTGAAA 
ACGTTCGCCG TGGATGAGAC CTCTGTGTCG GGCTACATCT ACCACAAACT GCTCGGGCAT 
GAGGTGGAGG ATGTGATCAT TAAGTGCCAG CTGCCAAAAC GCTTTACGGC ACAGGGGCTC 
CCCGACCTCA ACCATTCTCA GGTTTATGCT GTGAAGACTG TGCTGCAAAG ACCGCTGAGC 
CTGATCCAGG GACCACCAGG CACAGGGAAG ACAGTGACCT CTGCCACCAT CGTCTATCAC 
CTTGCTCGGC AGGGCAATGG GCCTGTGCTC GTCTGTGCTC CGAGTAACAT AGCTGTGGAT 
CAGCTGACTG AGAAGATCCA CCAGACTGGG CTGAAAGTTG TACGGCTTTG TGCCAAGAGC 
CGTGAGGCCA TCGACTCCCC AGTGTCATTC TTGGCCCTGC ACAACCAGAT CAGGAACATG 
GACAGCATGC CAGAGCTACA GAAGCTGCAG CAGCTGAAGG ATGAGACGGG GGAGCTGTCA 
TCTGCTGATG AGAAGCGGTA CCGGGCTCTG AAGCGTACCG CTGAGAGGGA GCTGCTCATG 
AATGCAGATG TCATCTGCTG CACATGTGTG GGTGCAGGGG ACCCAAGGCT TGCCAAGATG 
CAGTTCCGCT CCATTTTAAT TGACGAGAGC ACCCAGGCCA CTGAGCCAGA ATGCATGGTC 
CCTGTGGTCC TTGGTGCCAA GCAGCTCATC CTCGTGGGTG ACCACTGCCA GCTGGGCCCT 
GTGGTGATGT GCAAGAAAGC AGCCAAGGCT GGACTATCTC AGTCGCTCTT TGAGCGCCTG 
GTGGTGCTGG GCATCCGTCC CATCCGCCTC CAGGTCCAGT ATCGGATGCA CCCTGCGCTC 
AGTGCCTTCC CATCCAACAT ATTCTACGAG GGCTCTCTTC AGAATGGCGT CACTGCAGCT 
GATCGAGTGA AGAAGGGATT TGACTTCCAG TGGCCCCAAC CTGATAAGCC GATGTTCTTC 
TACGTGACCC AGGGCCAAGA GGAGATTGCT AGCTCAGGCA CCTCCTACCT GAACAGGACG 
GAAGCTGCAA ATGTGGAGAA GATCACCACG AAGCTGCTAA AGGCGGGTGC CAAGCCTGAC 
CAGATTGGCA TCATCACACC TTACGAGGGC CAGCGCTCCT ACCTGGTGCA GTACATGCAG 
TTCAGCGGCT CTCTGCACAC CAAGCTCTAC CAGGAGGTAG AGATTGCCAG CGTGGACGCA 
TTCCAGGGGC GAGAGAAGGA CTTCATCATT CTTTCCTGCG TGCGAGCCAA CGAGCACCAA 
GGCATTGGAT TTTTAAACGA CCCCAGGCGG CTTAATGTGG CCCTGACCAG AGCAAGGTAT 
GGGGTTATTA TTGTGGGCAA CCCAAAGGCC CTGTCCAAGC AGCCCCTCTG GAACCACCTG 
CTGAACTATT ACAAGGAGCA AAAGGTGCTG GTGGAGGGGC CCCTGAACAA CCTGCGGGAG 
AGCCTTATGC AGTTTAGCAA GCCCAGGAAG CTGGTCAATA CCATCAACCC GGGAGCCCGC 
TTCATGACGA CAGCCATGTA TGATGCCCGG GAGGCTATTA TCCCAGGGTC AGTCTATGAT 
CGGAGCAGCC AGGGCCGGCC CTCAAACATG TACTTCCAGA CCCACGACCA GATCGGCATG 
ATTAGTGCAG GCCCCAGCCA CGTGGCTGCC ATGAACATTC CCATCCCCTT CAACCTGGTC 
ATGCCCCCCA TGCCACCACC AGGATATTTT GGACAAGCAA ATGGGCCAGC CGCAGGCCGG 
GGTACCCCAA AAGGCAAGAC TGGTCGTGGG GGGCGCCAGA AGAACCGCTT TGGGCTCCCT 
GGACCCAGCC AGACGAATCT CCCCAACAGC CAGGCCAGCC AGGATGTGGC ATCGCAGCCC 
TTCTCCCAGG GCGCCCTGAC ACAGGGCTAC ATCTCCATGA GCCAGCCCTC CCAGATGAGC 
CAGCCTGGCC TCTCCCAGCC CGAGTTGTCC CAGGACAGTT ACCTTGGCGA TGAGTTTAAG 
TCACAGATTG ATGTGGCACT GTCGCAAGAC TCCACGTACC AAGGGGAGCG GGCATACCAG 
CACGGCGGAG TGACAGGGCT GTCCCAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632737.1
CDS	275..3667
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPVGAGAGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_005632680.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632680.2

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG GTAATGAGGA TTTAGTCATA 
ATTTGGTTAA GAGACATGCG GCTCATGCAG GGGGATGAGA TATGCCTGAG GTACAAAGGG 
GATCTGGCAC CCCTCTGGAA AGGGATCGGC CACGTCATCA AGGTCCCTGA TAATTATGGC 
GATGAGATCG CCATTGAGCT TCGGAGCAGT GTGGGTGCAC CTGTGGAGGT GACTCATAAC 
TTCCAGGTGG ATTTCGTATG GAAGTCAACC TCCTTTGATA GGATGCAGAG TGCGTTGAAA 
ACGTTCGCCG TGGATGAGAC CTCTGTGTCG GGCTACATCT ACCACAAACT GCTCGGGCAT 
GAGGTGGAGG ATGTGATCAT TAAGTGCCAG CTGCCAAAAC GCTTTACGGC ACAGGGGCTC 
CCCGACCTCA ACCATTCTCA GGTTTATGCT GTGAAGACTG TGCTGCAAAG ACCGCTGAGC 
CTGATCCAGG GACCACCAGG CACAGGGAAG ACAGTGACCT CTGCCACCAT CGTCTATCAC 
CTTGCTCGGC AGGGCAATGG GCCTGTGCTC GTCTGTGCTC CGAGTAACAT AGCTGTGGAT 
CAGCTGACTG AGAAGATCCA CCAGACTGGG CTGAAAGTTG TACGGCTTTG TGCCAAGAGC 
CGTGAGGCCA TCGACTCCCC AGTGTCATTC TTGGCCCTGC ACAACCAGAT CAGGAACATG 
GACAGCATGC CAGAGCTACA GAAGCTGCAG CAGCTGAAGG ATGAGACGGG GGAGCTGTCA 
TCTGCTGATG AGAAGCGGTA CCGGGCTCTG AAGCGTACCG CTGAGAGGGA GCTGCTCATG 
AATGCAGATG TCATCTGCTG CACATGTGTG GGTGCAGGGG ACCCAAGGCT TGCCAAGATG 
CAGTTCCGCT CCATTTTAAT TGACGAGAGC ACCCAGGCCA CTGAGCCAGA ATGCATGGTC 
CCTGTGGTCC TTGGTGCCAA GCAGCTCATC CTCGTGGGTG ACCACTGCCA GCTGGGCCCT 
GTGGTGATGT GCAAGAAAGC AGCCAAGGCT GGACTATCTC AGTCGCTCTT TGAGCGCCTG 
GTGGTGCTGG GCATCCGTCC CATCCGCCTC CAGGTCCAGT ATCGGATGCA CCCTGCGCTC 
AGTGCCTTCC CATCCAACAT ATTCTACGAG GGCTCTCTTC AGAATGGCGT CACTGCAGCT 
GATCGAGTGA AGAAGGGATT TGACTTCCAG TGGCCCCAAC CTGATAAGCC GATGTTCTTC 
TACGTGACCC AGGGCCAAGA GGAGATTGCT AGCTCAGGCA CCTCCTACCT GAACAGGACG 
GAAGCTGCAA ATGTGGAGAA GATCACCACG AAGCTGCTAA AGGCGGGTGC CAAGCCTGAC 
CAGATTGGCA TCATCACACC TTACGAGGGC CAGCGCTCCT ACCTGGTGCA GTACATGCAG 
TTCAGCGGCT CTCTGCACAC CAAGCTCTAC CAGGAGGTAG AGATTGCCAG CGTGGACGCA 
TTCCAGGGGC GAGAGAAGGA CTTCATCATT CTTTCCTGCG TGCGAGCCAA CGAGCACCAA 
GGCATTGGAT TTTTAAACGA CCCCAGGCGG CTTAATGTGG CCCTGACCAG AGCAAGGTAT 
GGGGTTATTA TTGTGGGCAA CCCAAAGGCC CTGTCCAAGC AGCCCCTCTG GAACCACCTG 
CTGAACTATT ACAAGGAGCA AAAGGTGCTG GTGGAGGGGC CCCTGAACAA CCTGCGGGAG 
AGCCTTATGC AGTTTAGCAA GCCCAGGAAG CTGGTCAATA CCATCAACCC GGGAGCCCGC 
TTCATGACGA CAGCCATGTA TGATGCCCGG GAGGCTATTA TCCCAGGGTC AGTCTATGAT 
CGGAGCAGCC AGGGCCGGCC CTCAAACATG TACTTCCAGA CCCACGACCA GATCGGCATG 
ATTAGTGCAG GCCCCAGCCA CGTGGCTGCC ATGAACATTC CCATCCCCTT CAACCTGGTC 
ATGCCCCCCA TGCCACCACC AGGATATTTT GGACAAGCAA ATGGGCCAGC CGCAGGCCGG 
GGTACCCCAA AAGGCAAGAC TGGTCGTGGG GGGCGCCAGA AGAACCGCTT TGGGCTCCCT 
GGACCCAGCC AGACGAATCT CCCCAACAGC CAGGCCAGCC AGGATGTGGC ATCGCAGCCC 
TTCTCCCAGG GCGCCCTGAC ACAGGGCTAC ATCTCCATGA GCCAGCCCTC CCAGATGAGC 
CAGCCTGGCC TCTCCCAGCC CGAGTTGTCC CAGGACAGTT ACCTTGGCGA TGAGTTTAAG 
TCACAGATTG ATGTGGCACT GTCGCAAGAC TCCACGTACC AAGGGGAGCG GGCATACCAG 
CACGGCGGAG TGACAGGGCT GTCCCAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_005632680.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632737.1 Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe21853
Clone ID Related Accession (Same CDS sequence)	XM_005632680.2 , XM_005632680.3
Accession Version	XM_005632680.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	regulator of nonsense transcripts 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005632680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG GTAATGAGGA TTTAGTCATA 
ATTTGGTTAA GAGACATGCG GCTCATGCAG GGGGATGAGA TATGCCTGAG GTACAAAGGG 
GATCTGGCAC CCCTCTGGAA AGGGATCGGC CACGTCATCA AGGTCCCTGA TAATTATGGC 
GATGAGATCG CCATTGAGCT TCGGAGCAGT GTGGGTGCAC CTGTGGAGGT GACTCATAAC 
TTCCAGGTGG ATTTCGTATG GAAGTCAACC TCCTTTGATA GGATGCAGAG TGCGTTGAAA 
ACGTTCGCCG TGGATGAGAC CTCTGTGTCG GGCTACATCT ACCACAAACT GCTCGGGCAT 
GAGGTGGAGG ATGTGATCAT TAAGTGCCAG CTGCCAAAAC GCTTTACGGC ACAGGGGCTC 
CCCGACCTCA ACCATTCTCA GGTTTATGCT GTGAAGACTG TGCTGCAAAG ACCGCTGAGC 
CTGATCCAGG GACCACCAGG CACAGGGAAG ACAGTGACCT CTGCCACCAT CGTCTATCAC 
CTTGCTCGGC AGGGCAATGG GCCTGTGCTC GTCTGTGCTC CGAGTAACAT AGCTGTGGAT 
CAGCTGACTG AGAAGATCCA CCAGACTGGG CTGAAAGTTG TACGGCTTTG TGCCAAGAGC 
CGTGAGGCCA TCGACTCCCC AGTGTCATTC TTGGCCCTGC ACAACCAGAT CAGGAACATG 
GACAGCATGC CAGAGCTACA GAAGCTGCAG CAGCTGAAGG ATGAGACGGG GGAGCTGTCA 
TCTGCTGATG AGAAGCGGTA CCGGGCTCTG AAGCGTACCG CTGAGAGGGA GCTGCTCATG 
AATGCAGATG TCATCTGCTG CACATGTGTG GGTGCAGGGG ACCCAAGGCT TGCCAAGATG 
CAGTTCCGCT CCATTTTAAT TGACGAGAGC ACCCAGGCCA CTGAGCCAGA ATGCATGGTC 
CCTGTGGTCC TTGGTGCCAA GCAGCTCATC CTCGTGGGTG ACCACTGCCA GCTGGGCCCT 
GTGGTGATGT GCAAGAAAGC AGCCAAGGCT GGACTATCTC AGTCGCTCTT TGAGCGCCTG 
GTGGTGCTGG GCATCCGTCC CATCCGCCTC CAGGTCCAGT ATCGGATGCA CCCTGCGCTC 
AGTGCCTTCC CATCCAACAT ATTCTACGAG GGCTCTCTTC AGAATGGCGT CACTGCAGCT 
GATCGAGTGA AGAAGGGATT TGACTTCCAG TGGCCCCAAC CTGATAAGCC GATGTTCTTC 
TACGTGACCC AGGGCCAAGA GGAGATTGCT AGCTCAGGCA CCTCCTACCT GAACAGGACG 
GAAGCTGCAA ATGTGGAGAA GATCACCACG AAGCTGCTAA AGGCGGGTGC CAAGCCTGAC 
CAGATTGGCA TCATCACACC TTACGAGGGC CAGCGCTCCT ACCTGGTGCA GTACATGCAG 
TTCAGCGGCT CTCTGCACAC CAAGCTCTAC CAGGAGGTAG AGATTGCCAG CGTGGACGCA 
TTCCAGGGGC GAGAGAAGGA CTTCATCATT CTTTCCTGCG TGCGAGCCAA CGAGCACCAA 
GGCATTGGAT TTTTAAACGA CCCCAGGCGG CTTAATGTGG CCCTGACCAG AGCAAGGTAT 
GGGGTTATTA TTGTGGGCAA CCCAAAGGCC CTGTCCAAGC AGCCCCTCTG GAACCACCTG 
CTGAACTATT ACAAGGAGCA AAAGGTGCTG GTGGAGGGGC CCCTGAACAA CCTGCGGGAG 
AGCCTTATGC AGTTTAGCAA GCCCAGGAAG CTGGTCAATA CCATCAACCC GGGAGCCCGC 
TTCATGACGA CAGCCATGTA TGATGCCCGG GAGGCTATTA TCCCAGGGTC AGTCTATGAT 
CGGAGCAGCC AGGGCCGGCC CTCAAACATG TACTTCCAGA CCCACGACCA GATCGGCATG 
ATTAGTGCAG GCCCCAGCCA CGTGGCTGCC ATGAACATTC CCATCCCCTT CAACCTGGTC 
ATGCCCCCCA TGCCACCACC AGGATATTTT GGACAAGCAA ATGGGCCAGC CGCAGGCCGG 
GGTACCCCAA AAGGCAAGAC TGGTCGTGGG GGGCGCCAGA AGAACCGCTT TGGGCTCCCT 
GGACCCAGCC AGACGAATCT CCCCAACAGC CAGGCCAGCC AGGATGTGGC ATCGCAGCCC 
TTCTCCCAGG GCGCCCTGAC ACAGGGCTAC ATCTCCATGA GCCAGCCCTC CCAGATGAGC 
CAGCCTGGCC TCTCCCAGCC CGAGTTGTCC CAGGACAGTT ACCTTGGCGA TGAGTTTAAG 
TCACAGATTG ATGTGGCACT GTCGCAAGAC TCCACGTACC AAGGGGAGCG GGCATACCAG 
CACGGCGGAG TGACAGGGCT GTCCCAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632737.1
CDS	212..3604
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPVGAGAGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_005632680.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632680.3

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG GTAATGAGGA TTTAGTCATA 
ATTTGGTTAA GAGACATGCG GCTCATGCAG GGGGATGAGA TATGCCTGAG GTACAAAGGG 
GATCTGGCAC CCCTCTGGAA AGGGATCGGC CACGTCATCA AGGTCCCTGA TAATTATGGC 
GATGAGATCG CCATTGAGCT TCGGAGCAGT GTGGGTGCAC CTGTGGAGGT GACTCATAAC 
TTCCAGGTGG ATTTCGTATG GAAGTCAACC TCCTTTGATA GGATGCAGAG TGCGTTGAAA 
ACGTTCGCCG TGGATGAGAC CTCTGTGTCG GGCTACATCT ACCACAAACT GCTCGGGCAT 
GAGGTGGAGG ATGTGATCAT TAAGTGCCAG CTGCCAAAAC GCTTTACGGC ACAGGGGCTC 
CCCGACCTCA ACCATTCTCA GGTTTATGCT GTGAAGACTG TGCTGCAAAG ACCGCTGAGC 
CTGATCCAGG GACCACCAGG CACAGGGAAG ACAGTGACCT CTGCCACCAT CGTCTATCAC 
CTTGCTCGGC AGGGCAATGG GCCTGTGCTC GTCTGTGCTC CGAGTAACAT AGCTGTGGAT 
CAGCTGACTG AGAAGATCCA CCAGACTGGG CTGAAAGTTG TACGGCTTTG TGCCAAGAGC 
CGTGAGGCCA TCGACTCCCC AGTGTCATTC TTGGCCCTGC ACAACCAGAT CAGGAACATG 
GACAGCATGC CAGAGCTACA GAAGCTGCAG CAGCTGAAGG ATGAGACGGG GGAGCTGTCA 
TCTGCTGATG AGAAGCGGTA CCGGGCTCTG AAGCGTACCG CTGAGAGGGA GCTGCTCATG 
AATGCAGATG TCATCTGCTG CACATGTGTG GGTGCAGGGG ACCCAAGGCT TGCCAAGATG 
CAGTTCCGCT CCATTTTAAT TGACGAGAGC ACCCAGGCCA CTGAGCCAGA ATGCATGGTC 
CCTGTGGTCC TTGGTGCCAA GCAGCTCATC CTCGTGGGTG ACCACTGCCA GCTGGGCCCT 
GTGGTGATGT GCAAGAAAGC AGCCAAGGCT GGACTATCTC AGTCGCTCTT TGAGCGCCTG 
GTGGTGCTGG GCATCCGTCC CATCCGCCTC CAGGTCCAGT ATCGGATGCA CCCTGCGCTC 
AGTGCCTTCC CATCCAACAT ATTCTACGAG GGCTCTCTTC AGAATGGCGT CACTGCAGCT 
GATCGAGTGA AGAAGGGATT TGACTTCCAG TGGCCCCAAC CTGATAAGCC GATGTTCTTC 
TACGTGACCC AGGGCCAAGA GGAGATTGCT AGCTCAGGCA CCTCCTACCT GAACAGGACG 
GAAGCTGCAA ATGTGGAGAA GATCACCACG AAGCTGCTAA AGGCGGGTGC CAAGCCTGAC 
CAGATTGGCA TCATCACACC TTACGAGGGC CAGCGCTCCT ACCTGGTGCA GTACATGCAG 
TTCAGCGGCT CTCTGCACAC CAAGCTCTAC CAGGAGGTAG AGATTGCCAG CGTGGACGCA 
TTCCAGGGGC GAGAGAAGGA CTTCATCATT CTTTCCTGCG TGCGAGCCAA CGAGCACCAA 
GGCATTGGAT TTTTAAACGA CCCCAGGCGG CTTAATGTGG CCCTGACCAG AGCAAGGTAT 
GGGGTTATTA TTGTGGGCAA CCCAAAGGCC CTGTCCAAGC AGCCCCTCTG GAACCACCTG 
CTGAACTATT ACAAGGAGCA AAAGGTGCTG GTGGAGGGGC CCCTGAACAA CCTGCGGGAG 
AGCCTTATGC AGTTTAGCAA GCCCAGGAAG CTGGTCAATA CCATCAACCC GGGAGCCCGC 
TTCATGACGA CAGCCATGTA TGATGCCCGG GAGGCTATTA TCCCAGGGTC AGTCTATGAT 
CGGAGCAGCC AGGGCCGGCC CTCAAACATG TACTTCCAGA CCCACGACCA GATCGGCATG 
ATTAGTGCAG GCCCCAGCCA CGTGGCTGCC ATGAACATTC CCATCCCCTT CAACCTGGTC 
ATGCCCCCCA TGCCACCACC AGGATATTTT GGACAAGCAA ATGGGCCAGC CGCAGGCCGG 
GGTACCCCAA AAGGCAAGAC TGGTCGTGGG GGGCGCCAGA AGAACCGCTT TGGGCTCCCT 
GGACCCAGCC AGACGAATCT CCCCAACAGC CAGGCCAGCC AGGATGTGGC ATCGCAGCCC 
TTCTCCCAGG GCGCCCTGAC ACAGGGCTAC ATCTCCATGA GCCAGCCCTC CCAGATGAGC 
CAGCCTGGCC TCTCCCAGCC CGAGTTGTCC CAGGACAGTT ACCTTGGCGA TGAGTTTAAG 
TCACAGATTG ATGTGGCACT GTCGCAAGAC TCCACGTACC AAGGGGAGCG GGCATACCAG 
CACGGCGGAG TGACAGGGCT GTCCCAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_533868.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533868.2 Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X4, mRNA.

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CloneID	OCe21854
Clone ID Related Accession (Same CDS sequence)	XM_533868.5 , XM_533868.4
Accession Version	XM_533868.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	regulator of nonsense transcripts 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_533868.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG ACATGCGGCT CATGCAGGGG 
GATGAGATAT GCCTGAGGTA CAAAGGGGAT CTGGCACCCC TCTGGAAAGG GATCGGCCAC 
GTCATCAAGG TCCCTGATAA TTATGGCGAT GAGATCGCCA TTGAGCTTCG GAGCAGTGTG 
GGTGCACCTG TGGAGGTGAC TCATAACTTC CAGGTGGATT TCGTATGGAA GTCAACCTCC 
TTTGATAGGA TGCAGAGTGC GTTGAAAACG TTCGCCGTGG ATGAGACCTC TGTGTCGGGC 
TACATCTACC ACAAACTGCT CGGGCATGAG GTGGAGGATG TGATCATTAA GTGCCAGCTG 
CCAAAACGCT TTACGGCACA GGGGCTCCCC GACCTCAACC ATTCTCAGGT TTATGCTGTG 
AAGACTGTGC TGCAAAGACC GCTGAGCCTG ATCCAGGGAC CACCAGGCAC AGGGAAGACA 
GTGACCTCTG CCACCATCGT CTATCACCTT GCTCGGCAGG GCAATGGGCC TGTGCTCGTC 
TGTGCTCCGA GTAACATAGC TGTGGATCAG CTGACTGAGA AGATCCACCA GACTGGGCTG 
AAAGTTGTAC GGCTTTGTGC CAAGAGCCGT GAGGCCATCG ACTCCCCAGT GTCATTCTTG 
GCCCTGCACA ACCAGATCAG GAACATGGAC AGCATGCCAG AGCTACAGAA GCTGCAGCAG 
CTGAAGGATG AGACGGGGGA GCTGTCATCT GCTGATGAGA AGCGGTACCG GGCTCTGAAG 
CGTACCGCTG AGAGGGAGCT GCTCATGAAT GCAGATGTCA TCTGCTGCAC ATGTGTGGGT 
GCAGGGGACC CAAGGCTTGC CAAGATGCAG TTCCGCTCCA TTTTAATTGA CGAGAGCACC 
CAGGCCACTG AGCCAGAATG CATGGTCCCT GTGGTCCTTG GTGCCAAGCA GCTCATCCTC 
GTGGGTGACC ACTGCCAGCT GGGCCCTGTG GTGATGTGCA AGAAAGCAGC CAAGGCTGGA 
CTATCTCAGT CGCTCTTTGA GCGCCTGGTG GTGCTGGGCA TCCGTCCCAT CCGCCTCCAG 
GTCCAGTATC GGATGCACCC TGCGCTCAGT GCCTTCCCAT CCAACATATT CTACGAGGGC 
TCTCTTCAGA ATGGCGTCAC TGCAGCTGAT CGAGTGAAGA AGGGATTTGA CTTCCAGTGG 
CCCCAACCTG ATAAGCCGAT GTTCTTCTAC GTGACCCAGG GCCAAGAGGA GATTGCTAGC 
TCAGGCACCT CCTACCTGAA CAGGACGGAA GCTGCAAATG TGGAGAAGAT CACCACGAAG 
CTGCTAAAGG CGGGTGCCAA GCCTGACCAG ATTGGCATCA TCACACCTTA CGAGGGCCAG 
CGCTCCTACC TGGTGCAGTA CATGCAGTTC AGCGGCTCTC TGCACACCAA GCTCTACCAG 
GAGGTAGAGA TTGCCAGCGT GGACGCATTC CAGGGGCGAG AGAAGGACTT CATCATTCTT 
TCCTGCGTGC GAGCCAACGA GCACCAAGGC ATTGGATTTT TAAACGACCC CAGGCGGCTT 
AATGTGGCCC TGACCAGAGC AAGGTATGGG GTTATTATTG TGGGCAACCC AAAGGCCCTG 
TCCAAGCAGC CCCTCTGGAA CCACCTGCTG AACTATTACA AGGAGCAAAA GGTGCTGGTG 
GAGGGGCCCC TGAACAACCT GCGGGAGAGC CTTATGCAGT TTAGCAAGCC CAGGAAGCTG 
GTCAATACCA TCAACCCGGG AGCCCGCTTC ATGACGACAG CCATGTATGA TGCCCGGGAG 
GCTATTATCC CAGGGTCAGT CTATGATCGG AGCAGCCAGG GCCGGCCCTC AAACATGTAC 
TTCCAGACCC ACGACCAGAT CGGCATGATT AGTGCAGGCC CCAGCCACGT GGCTGCCATG 
AACATTCCCA TCCCCTTCAA CCTGGTCATG CCCCCCATGC CACCACCAGG ATATTTTGGA 
CAAGCAAATG GGCCAGCCGC AGGCCGGGGT ACCCCAAAAG GCAAGACTGG TCGTGGGGGG 
CGCCAGAAGA ACCGCTTTGG GCTCCCTGGA CCCAGCCAGA CGAATCTCCC CAACAGCCAG 
GCCAGCCAGG ATGTGGCATC GCAGCCCTTC TCCCAGGGCG CCCTGACACA GGGCTACATC 
TCCATGAGCC AGCCCTCCCA GATGAGCCAG CCTGGCCTCT CCCAGCCCGA GTTGTCCCAG 
GACAGTTACC TTGGCGATGA GTTTAAGTCA CAGATTGATG TGGCACTGTC GCAAGACTCC 
ACGTACCAAG GGGAGCGGGC ATACCAGCAC GGCGGAGTGA CAGGGCTGTC CCAGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533868.2
CDS	210..3569
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPVGAGAGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_533868.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533868.5

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG ACATGCGGCT CATGCAGGGG 
GATGAGATAT GCCTGAGGTA CAAAGGGGAT CTGGCACCCC TCTGGAAAGG GATCGGCCAC 
GTCATCAAGG TCCCTGATAA TTATGGCGAT GAGATCGCCA TTGAGCTTCG GAGCAGTGTG 
GGTGCACCTG TGGAGGTGAC TCATAACTTC CAGGTGGATT TCGTATGGAA GTCAACCTCC 
TTTGATAGGA TGCAGAGTGC GTTGAAAACG TTCGCCGTGG ATGAGACCTC TGTGTCGGGC 
TACATCTACC ACAAACTGCT CGGGCATGAG GTGGAGGATG TGATCATTAA GTGCCAGCTG 
CCAAAACGCT TTACGGCACA GGGGCTCCCC GACCTCAACC ATTCTCAGGT TTATGCTGTG 
AAGACTGTGC TGCAAAGACC GCTGAGCCTG ATCCAGGGAC CACCAGGCAC AGGGAAGACA 
GTGACCTCTG CCACCATCGT CTATCACCTT GCTCGGCAGG GCAATGGGCC TGTGCTCGTC 
TGTGCTCCGA GTAACATAGC TGTGGATCAG CTGACTGAGA AGATCCACCA GACTGGGCTG 
AAAGTTGTAC GGCTTTGTGC CAAGAGCCGT GAGGCCATCG ACTCCCCAGT GTCATTCTTG 
GCCCTGCACA ACCAGATCAG GAACATGGAC AGCATGCCAG AGCTACAGAA GCTGCAGCAG 
CTGAAGGATG AGACGGGGGA GCTGTCATCT GCTGATGAGA AGCGGTACCG GGCTCTGAAG 
CGTACCGCTG AGAGGGAGCT GCTCATGAAT GCAGATGTCA TCTGCTGCAC ATGTGTGGGT 
GCAGGGGACC CAAGGCTTGC CAAGATGCAG TTCCGCTCCA TTTTAATTGA CGAGAGCACC 
CAGGCCACTG AGCCAGAATG CATGGTCCCT GTGGTCCTTG GTGCCAAGCA GCTCATCCTC 
GTGGGTGACC ACTGCCAGCT GGGCCCTGTG GTGATGTGCA AGAAAGCAGC CAAGGCTGGA 
CTATCTCAGT CGCTCTTTGA GCGCCTGGTG GTGCTGGGCA TCCGTCCCAT CCGCCTCCAG 
GTCCAGTATC GGATGCACCC TGCGCTCAGT GCCTTCCCAT CCAACATATT CTACGAGGGC 
TCTCTTCAGA ATGGCGTCAC TGCAGCTGAT CGAGTGAAGA AGGGATTTGA CTTCCAGTGG 
CCCCAACCTG ATAAGCCGAT GTTCTTCTAC GTGACCCAGG GCCAAGAGGA GATTGCTAGC 
TCAGGCACCT CCTACCTGAA CAGGACGGAA GCTGCAAATG TGGAGAAGAT CACCACGAAG 
CTGCTAAAGG CGGGTGCCAA GCCTGACCAG ATTGGCATCA TCACACCTTA CGAGGGCCAG 
CGCTCCTACC TGGTGCAGTA CATGCAGTTC AGCGGCTCTC TGCACACCAA GCTCTACCAG 
GAGGTAGAGA TTGCCAGCGT GGACGCATTC CAGGGGCGAG AGAAGGACTT CATCATTCTT 
TCCTGCGTGC GAGCCAACGA GCACCAAGGC ATTGGATTTT TAAACGACCC CAGGCGGCTT 
AATGTGGCCC TGACCAGAGC AAGGTATGGG GTTATTATTG TGGGCAACCC AAAGGCCCTG 
TCCAAGCAGC CCCTCTGGAA CCACCTGCTG AACTATTACA AGGAGCAAAA GGTGCTGGTG 
GAGGGGCCCC TGAACAACCT GCGGGAGAGC CTTATGCAGT TTAGCAAGCC CAGGAAGCTG 
GTCAATACCA TCAACCCGGG AGCCCGCTTC ATGACGACAG CCATGTATGA TGCCCGGGAG 
GCTATTATCC CAGGGTCAGT CTATGATCGG AGCAGCCAGG GCCGGCCCTC AAACATGTAC 
TTCCAGACCC ACGACCAGAT CGGCATGATT AGTGCAGGCC CCAGCCACGT GGCTGCCATG 
AACATTCCCA TCCCCTTCAA CCTGGTCATG CCCCCCATGC CACCACCAGG ATATTTTGGA 
CAAGCAAATG GGCCAGCCGC AGGCCGGGGT ACCCCAAAAG GCAAGACTGG TCGTGGGGGG 
CGCCAGAAGA ACCGCTTTGG GCTCCCTGGA CCCAGCCAGA CGAATCTCCC CAACAGCCAG 
GCCAGCCAGG ATGTGGCATC GCAGCCCTTC TCCCAGGGCG CCCTGACACA GGGCTACATC 
TCCATGAGCC AGCCCTCCCA GATGAGCCAG CCTGGCCTCT CCCAGCCCGA GTTGTCCCAG 
GACAGTTACC TTGGCGATGA GTTTAAGTCA CAGATTGATG TGGCACTGTC GCAAGACTCC 
ACGTACCAAG GGGAGCGGGC ATACCAGCAC GGCGGAGTGA CAGGGCTGTC CCAGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_533868.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533868.2 Canis lupus familiaris UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.

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CloneID	OCe21854
Clone ID Related Accession (Same CDS sequence)	XM_533868.5 , XM_533868.4
Accession Version	XM_533868.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	regulator of nonsense transcripts 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533868.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG ACATGCGGCT CATGCAGGGG 
GATGAGATAT GCCTGAGGTA CAAAGGGGAT CTGGCACCCC TCTGGAAAGG GATCGGCCAC 
GTCATCAAGG TCCCTGATAA TTATGGCGAT GAGATCGCCA TTGAGCTTCG GAGCAGTGTG 
GGTGCACCTG TGGAGGTGAC TCATAACTTC CAGGTGGATT TCGTATGGAA GTCAACCTCC 
TTTGATAGGA TGCAGAGTGC GTTGAAAACG TTCGCCGTGG ATGAGACCTC TGTGTCGGGC 
TACATCTACC ACAAACTGCT CGGGCATGAG GTGGAGGATG TGATCATTAA GTGCCAGCTG 
CCAAAACGCT TTACGGCACA GGGGCTCCCC GACCTCAACC ATTCTCAGGT TTATGCTGTG 
AAGACTGTGC TGCAAAGACC GCTGAGCCTG ATCCAGGGAC CACCAGGCAC AGGGAAGACA 
GTGACCTCTG CCACCATCGT CTATCACCTT GCTCGGCAGG GCAATGGGCC TGTGCTCGTC 
TGTGCTCCGA GTAACATAGC TGTGGATCAG CTGACTGAGA AGATCCACCA GACTGGGCTG 
AAAGTTGTAC GGCTTTGTGC CAAGAGCCGT GAGGCCATCG ACTCCCCAGT GTCATTCTTG 
GCCCTGCACA ACCAGATCAG GAACATGGAC AGCATGCCAG AGCTACAGAA GCTGCAGCAG 
CTGAAGGATG AGACGGGGGA GCTGTCATCT GCTGATGAGA AGCGGTACCG GGCTCTGAAG 
CGTACCGCTG AGAGGGAGCT GCTCATGAAT GCAGATGTCA TCTGCTGCAC ATGTGTGGGT 
GCAGGGGACC CAAGGCTTGC CAAGATGCAG TTCCGCTCCA TTTTAATTGA CGAGAGCACC 
CAGGCCACTG AGCCAGAATG CATGGTCCCT GTGGTCCTTG GTGCCAAGCA GCTCATCCTC 
GTGGGTGACC ACTGCCAGCT GGGCCCTGTG GTGATGTGCA AGAAAGCAGC CAAGGCTGGA 
CTATCTCAGT CGCTCTTTGA GCGCCTGGTG GTGCTGGGCA TCCGTCCCAT CCGCCTCCAG 
GTCCAGTATC GGATGCACCC TGCGCTCAGT GCCTTCCCAT CCAACATATT CTACGAGGGC 
TCTCTTCAGA ATGGCGTCAC TGCAGCTGAT CGAGTGAAGA AGGGATTTGA CTTCCAGTGG 
CCCCAACCTG ATAAGCCGAT GTTCTTCTAC GTGACCCAGG GCCAAGAGGA GATTGCTAGC 
TCAGGCACCT CCTACCTGAA CAGGACGGAA GCTGCAAATG TGGAGAAGAT CACCACGAAG 
CTGCTAAAGG CGGGTGCCAA GCCTGACCAG ATTGGCATCA TCACACCTTA CGAGGGCCAG 
CGCTCCTACC TGGTGCAGTA CATGCAGTTC AGCGGCTCTC TGCACACCAA GCTCTACCAG 
GAGGTAGAGA TTGCCAGCGT GGACGCATTC CAGGGGCGAG AGAAGGACTT CATCATTCTT 
TCCTGCGTGC GAGCCAACGA GCACCAAGGC ATTGGATTTT TAAACGACCC CAGGCGGCTT 
AATGTGGCCC TGACCAGAGC AAGGTATGGG GTTATTATTG TGGGCAACCC AAAGGCCCTG 
TCCAAGCAGC CCCTCTGGAA CCACCTGCTG AACTATTACA AGGAGCAAAA GGTGCTGGTG 
GAGGGGCCCC TGAACAACCT GCGGGAGAGC CTTATGCAGT TTAGCAAGCC CAGGAAGCTG 
GTCAATACCA TCAACCCGGG AGCCCGCTTC ATGACGACAG CCATGTATGA TGCCCGGGAG 
GCTATTATCC CAGGGTCAGT CTATGATCGG AGCAGCCAGG GCCGGCCCTC AAACATGTAC 
TTCCAGACCC ACGACCAGAT CGGCATGATT AGTGCAGGCC CCAGCCACGT GGCTGCCATG 
AACATTCCCA TCCCCTTCAA CCTGGTCATG CCCCCCATGC CACCACCAGG ATATTTTGGA 
CAAGCAAATG GGCCAGCCGC AGGCCGGGGT ACCCCAAAAG GCAAGACTGG TCGTGGGGGG 
CGCCAGAAGA ACCGCTTTGG GCTCCCTGGA CCCAGCCAGA CGAATCTCCC CAACAGCCAG 
GCCAGCCAGG ATGTGGCATC GCAGCCCTTC TCCCAGGGCG CCCTGACACA GGGCTACATC 
TCCATGAGCC AGCCCTCCCA GATGAGCCAG CCTGGCCTCT CCCAGCCCGA GTTGTCCCAG 
GACAGTTACC TTGGCGATGA GTTTAAGTCA CAGATTGATG TGGCACTGTC GCAAGACTCC 
ACGTACCAAG GGGAGCGGGC ATACCAGCAC GGCGGAGTGA CAGGGCTGTC CCAGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533868.2
CDS	274..3633
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPVGAGAGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_533868.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533868.4

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG ACATGCGGCT CATGCAGGGG 
GATGAGATAT GCCTGAGGTA CAAAGGGGAT CTGGCACCCC TCTGGAAAGG GATCGGCCAC 
GTCATCAAGG TCCCTGATAA TTATGGCGAT GAGATCGCCA TTGAGCTTCG GAGCAGTGTG 
GGTGCACCTG TGGAGGTGAC TCATAACTTC CAGGTGGATT TCGTATGGAA GTCAACCTCC 
TTTGATAGGA TGCAGAGTGC GTTGAAAACG TTCGCCGTGG ATGAGACCTC TGTGTCGGGC 
TACATCTACC ACAAACTGCT CGGGCATGAG GTGGAGGATG TGATCATTAA GTGCCAGCTG 
CCAAAACGCT TTACGGCACA GGGGCTCCCC GACCTCAACC ATTCTCAGGT TTATGCTGTG 
AAGACTGTGC TGCAAAGACC GCTGAGCCTG ATCCAGGGAC CACCAGGCAC AGGGAAGACA 
GTGACCTCTG CCACCATCGT CTATCACCTT GCTCGGCAGG GCAATGGGCC TGTGCTCGTC 
TGTGCTCCGA GTAACATAGC TGTGGATCAG CTGACTGAGA AGATCCACCA GACTGGGCTG 
AAAGTTGTAC GGCTTTGTGC CAAGAGCCGT GAGGCCATCG ACTCCCCAGT GTCATTCTTG 
GCCCTGCACA ACCAGATCAG GAACATGGAC AGCATGCCAG AGCTACAGAA GCTGCAGCAG 
CTGAAGGATG AGACGGGGGA GCTGTCATCT GCTGATGAGA AGCGGTACCG GGCTCTGAAG 
CGTACCGCTG AGAGGGAGCT GCTCATGAAT GCAGATGTCA TCTGCTGCAC ATGTGTGGGT 
GCAGGGGACC CAAGGCTTGC CAAGATGCAG TTCCGCTCCA TTTTAATTGA CGAGAGCACC 
CAGGCCACTG AGCCAGAATG CATGGTCCCT GTGGTCCTTG GTGCCAAGCA GCTCATCCTC 
GTGGGTGACC ACTGCCAGCT GGGCCCTGTG GTGATGTGCA AGAAAGCAGC CAAGGCTGGA 
CTATCTCAGT CGCTCTTTGA GCGCCTGGTG GTGCTGGGCA TCCGTCCCAT CCGCCTCCAG 
GTCCAGTATC GGATGCACCC TGCGCTCAGT GCCTTCCCAT CCAACATATT CTACGAGGGC 
TCTCTTCAGA ATGGCGTCAC TGCAGCTGAT CGAGTGAAGA AGGGATTTGA CTTCCAGTGG 
CCCCAACCTG ATAAGCCGAT GTTCTTCTAC GTGACCCAGG GCCAAGAGGA GATTGCTAGC 
TCAGGCACCT CCTACCTGAA CAGGACGGAA GCTGCAAATG TGGAGAAGAT CACCACGAAG 
CTGCTAAAGG CGGGTGCCAA GCCTGACCAG ATTGGCATCA TCACACCTTA CGAGGGCCAG 
CGCTCCTACC TGGTGCAGTA CATGCAGTTC AGCGGCTCTC TGCACACCAA GCTCTACCAG 
GAGGTAGAGA TTGCCAGCGT GGACGCATTC CAGGGGCGAG AGAAGGACTT CATCATTCTT 
TCCTGCGTGC GAGCCAACGA GCACCAAGGC ATTGGATTTT TAAACGACCC CAGGCGGCTT 
AATGTGGCCC TGACCAGAGC AAGGTATGGG GTTATTATTG TGGGCAACCC AAAGGCCCTG 
TCCAAGCAGC CCCTCTGGAA CCACCTGCTG AACTATTACA AGGAGCAAAA GGTGCTGGTG 
GAGGGGCCCC TGAACAACCT GCGGGAGAGC CTTATGCAGT TTAGCAAGCC CAGGAAGCTG 
GTCAATACCA TCAACCCGGG AGCCCGCTTC ATGACGACAG CCATGTATGA TGCCCGGGAG 
GCTATTATCC CAGGGTCAGT CTATGATCGG AGCAGCCAGG GCCGGCCCTC AAACATGTAC 
TTCCAGACCC ACGACCAGAT CGGCATGATT AGTGCAGGCC CCAGCCACGT GGCTGCCATG 
AACATTCCCA TCCCCTTCAA CCTGGTCATG CCCCCCATGC CACCACCAGG ATATTTTGGA 
CAAGCAAATG GGCCAGCCGC AGGCCGGGGT ACCCCAAAAG GCAAGACTGG TCGTGGGGGG 
CGCCAGAAGA ACCGCTTTGG GCTCCCTGGA CCCAGCCAGA CGAATCTCCC CAACAGCCAG 
GCCAGCCAGG ATGTGGCATC GCAGCCCTTC TCCCAGGGCG CCCTGACACA GGGCTACATC 
TCCATGAGCC AGCCCTCCCA GATGAGCCAG CCTGGCCTCT CCCAGCCCGA GTTGTCCCAG 
GACAGTTACC TTGGCGATGA GTTTAAGTCA CAGATTGATG TGGCACTGTC GCAAGACTCC 
ACGTACCAAG GGGAGCGGGC ATACCAGCAC GGCGGAGTGA CAGGGCTGTC CCAGTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_022406837.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262545.1 Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X1, mRNA.

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CloneID	OCe295500
Clone ID Related Accession (Same CDS sequence)	XM_022406837.1
Accession Version	XM_022406837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG GTAATGAGGA TTTAGTCATA 
ATTTGGTTAA GAGACATGCG GCTCATGCAG GGGGATGAGA TATGCCTGAG GTACAAAGGG 
GATCTGGCAC CCCTCTGGAA AGGGATCGGC CACGTCATCA AGGTCCCTGA TAATTATGGC 
GATGAGATCG CCATTGAGCT TCGGAGCAGT GTGGGTGCAC CTGTGGAGGT GACTCATAAC 
TTCCAGGTGG ATTTCGTATG GAAGTCAACC TCCTTTGATA GGATGCAGAG TGCGTTGAAA 
ACGTTCGCCG TGGATGAGAC CTCTGTGTCG GGCTACATCT ACCACAAACT GCTCGGGCAT 
GAGGTGGAGG ATGTGATCAT TAAGTGCCAG CTGCCAAAAC GCTTTACGGC ACAGGGGCTC 
CCCGACCTCA ACCATTCTCA GGTTTATGCT GTGAAGACTG TGCTGCAAAG ACCGCTGAGC 
CTGATCCAGG GACCACCAGG CACAGGGAAG ACAGTGACCT CTGCCACCAT CGTCTATCAC 
CTTGCTCGGC AGGGCAATGG GCCTGTGCTC GTCTGTGCTC CGAGTAACAT AGCTGTGGAT 
CAGCTGACTG AGAAGATCCA CCAGACTGGG CTGAAAGTTG TACGGCTTTG TGCCAAGAGC 
CGTGAGGCCA TCGACTCCCC AGTGTCATTC TTGGCCCTGC ACAACCAGAT CAGGAACATG 
GACAGCATGC CAGAGCTACA GAAGCTGCAG CAGCTGAAGG ATGAGACGGG GGAGCTGTCA 
TCTGCTGATG AGAAGCGGTA CCGGGCTCTG AAGCGTACCG CTGAGAGGGA GCTGCTCATG 
AATGCAGATG TCATCTGCTG CACATGTGTG GGTGCAGGGG ACCCAAGGCT TGCCAAGATG 
CAGTTCCGCT CCATTTTAAT TGACGAGAGC ACCCAGGCCA CTGAGCCAGA ATGCATGGTC 
CCTGTGGTCC TTGGTGCCAA GCAGCTCATC CTCGTGGGTG ACCACTGCCA GCTGGGCCCT 
GTGGTGATGT GCAAGAAAGC AGCCAAGGCT GGACTATCTC AGTCGCTCTT TGAGCGCCTG 
GTGGTGCTGG GCATCCGTCC CATCCGCCTC CAGGTCCAGT ATCGGATGCA CCCTGCGCTC 
AGTGCCTTCC CATCCAACAT ATTCTACGAG GGCTCTCTTC AGAATGGCGT CACTGCAGCT 
GATCGAGTGA AGAAGGGATT TGACTTCCAG TGGCCCCAAC CTGATAAGCC GATGTTCTTC 
TACGTGACCC AGGGCCAAGA GGAGATTGCT AGCTCAGGCA CCTCCTACCT GAACAGGACG 
GAAGCTGCAA ATGTGGAGAA GATCACCACG AAGCTGCTAA AGGCGGGTGC CAAGCCTGAC 
CAGATTGGCA TCATCACACC TTACGAGGGC CAGCGCTCCT ACCTGGTGCA GTACATGCAG 
TTCAGCGGCT CTCTGCACAC CAAGCTCTAC CAGGAGGTAG AGATTGCCAG CGTGGACGCA 
TTCCAGGGGC GAGAGAAGGA CTTCATCATT CTTTCCTGCG TGCGAGCCAA CGAGCACCAA 
GGCATTGGAT TTTTAAACGA CCCCAGGCGG CTTAATGTGG CCCTGACCAG AGCAAGGTAT 
GGGGTTATTA TTGTGGGCAA CCCAAAGGCC CTGTCCAAGC AGCCCCTCTG GAACCACCTG 
CTGAACTATT ACAAGGAGCA AAAGGTGCTG GTGGAGGGGC CCCTGAACAA CCTGCGGGAG 
AGCCTTATGC AGTTTAGCAA GCCCAGGAAG CTGGTCAATA CCATCAACCC GGGAGCCCGC 
TTCATGACGA CAGCCATGTA TGATGCCCGG GAGGCTATTA TCCCAGGGTC AGTCTATGAT 
CGGAGCAGCC AGGGCCGGCC CTCAAACATG TACTTCCAGA CCCACGACCA GATCGGCATG 
ATTAGTGCAG GCCCCAGCCA CGTGGCTGCC ATGAACATTC CCATCCCCTT CAACCTGGTC 
ATGCCCCCCA TGCCACCACC AGGATATTTT GGACAAGCAA ATGGGCCAGC CGCAGGAACC 
CTGGACTTCT GTCTTTCAGG CCGGGGTACC CCAAAAGGCA AGACTGGTCG TGGGGGGCGC 
CAGAAGAACC GCTTTGGGCT CCCTGGACCC AGCCAGACGA ATCTCCCCAA CAGCCAGGCC 
AGCCAGGATG TGGCATCGCA GCCCTTCTCC CAGGGCGCCC TGACACAGGG CTACATCTCC 
ATGAGCCAGC CCTCCCAGAT GAGCCAGCCT GGCCTCTCCC AGCCCGAGTT GTCCCAGGAC 
AGTTACCTTG GCGATGAGTT TAAGTCACAG ATTGATGTGG CACTGTCGCA AGACTCCACG 
TACCAAGGGG AGCGGGCATA CCAGCACGGC GGAGTGACAG GGCTGTCCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262545.1
CDS	214..3630
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPVGAGAGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGTLDFCLSGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_022406837.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406837.1

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG GTAATGAGGA TTTAGTCATA 
ATTTGGTTAA GAGACATGCG GCTCATGCAG GGGGATGAGA TATGCCTGAG GTACAAAGGG 
GATCTGGCAC CCCTCTGGAA AGGGATCGGC CACGTCATCA AGGTCCCTGA TAATTATGGC 
GATGAGATCG CCATTGAGCT TCGGAGCAGT GTGGGTGCAC CTGTGGAGGT GACTCATAAC 
TTCCAGGTGG ATTTCGTATG GAAGTCAACC TCCTTTGATA GGATGCAGAG TGCGTTGAAA 
ACGTTCGCCG TGGATGAGAC CTCTGTGTCG GGCTACATCT ACCACAAACT GCTCGGGCAT 
GAGGTGGAGG ATGTGATCAT TAAGTGCCAG CTGCCAAAAC GCTTTACGGC ACAGGGGCTC 
CCCGACCTCA ACCATTCTCA GGTTTATGCT GTGAAGACTG TGCTGCAAAG ACCGCTGAGC 
CTGATCCAGG GACCACCAGG CACAGGGAAG ACAGTGACCT CTGCCACCAT CGTCTATCAC 
CTTGCTCGGC AGGGCAATGG GCCTGTGCTC GTCTGTGCTC CGAGTAACAT AGCTGTGGAT 
CAGCTGACTG AGAAGATCCA CCAGACTGGG CTGAAAGTTG TACGGCTTTG TGCCAAGAGC 
CGTGAGGCCA TCGACTCCCC AGTGTCATTC TTGGCCCTGC ACAACCAGAT CAGGAACATG 
GACAGCATGC CAGAGCTACA GAAGCTGCAG CAGCTGAAGG ATGAGACGGG GGAGCTGTCA 
TCTGCTGATG AGAAGCGGTA CCGGGCTCTG AAGCGTACCG CTGAGAGGGA GCTGCTCATG 
AATGCAGATG TCATCTGCTG CACATGTGTG GGTGCAGGGG ACCCAAGGCT TGCCAAGATG 
CAGTTCCGCT CCATTTTAAT TGACGAGAGC ACCCAGGCCA CTGAGCCAGA ATGCATGGTC 
CCTGTGGTCC TTGGTGCCAA GCAGCTCATC CTCGTGGGTG ACCACTGCCA GCTGGGCCCT 
GTGGTGATGT GCAAGAAAGC AGCCAAGGCT GGACTATCTC AGTCGCTCTT TGAGCGCCTG 
GTGGTGCTGG GCATCCGTCC CATCCGCCTC CAGGTCCAGT ATCGGATGCA CCCTGCGCTC 
AGTGCCTTCC CATCCAACAT ATTCTACGAG GGCTCTCTTC AGAATGGCGT CACTGCAGCT 
GATCGAGTGA AGAAGGGATT TGACTTCCAG TGGCCCCAAC CTGATAAGCC GATGTTCTTC 
TACGTGACCC AGGGCCAAGA GGAGATTGCT AGCTCAGGCA CCTCCTACCT GAACAGGACG 
GAAGCTGCAA ATGTGGAGAA GATCACCACG AAGCTGCTAA AGGCGGGTGC CAAGCCTGAC 
CAGATTGGCA TCATCACACC TTACGAGGGC CAGCGCTCCT ACCTGGTGCA GTACATGCAG 
TTCAGCGGCT CTCTGCACAC CAAGCTCTAC CAGGAGGTAG AGATTGCCAG CGTGGACGCA 
TTCCAGGGGC GAGAGAAGGA CTTCATCATT CTTTCCTGCG TGCGAGCCAA CGAGCACCAA 
GGCATTGGAT TTTTAAACGA CCCCAGGCGG CTTAATGTGG CCCTGACCAG AGCAAGGTAT 
GGGGTTATTA TTGTGGGCAA CCCAAAGGCC CTGTCCAAGC AGCCCCTCTG GAACCACCTG 
CTGAACTATT ACAAGGAGCA AAAGGTGCTG GTGGAGGGGC CCCTGAACAA CCTGCGGGAG 
AGCCTTATGC AGTTTAGCAA GCCCAGGAAG CTGGTCAATA CCATCAACCC GGGAGCCCGC 
TTCATGACGA CAGCCATGTA TGATGCCCGG GAGGCTATTA TCCCAGGGTC AGTCTATGAT 
CGGAGCAGCC AGGGCCGGCC CTCAAACATG TACTTCCAGA CCCACGACCA GATCGGCATG 
ATTAGTGCAG GCCCCAGCCA CGTGGCTGCC ATGAACATTC CCATCCCCTT CAACCTGGTC 
ATGCCCCCCA TGCCACCACC AGGATATTTT GGACAAGCAA ATGGGCCAGC CGCAGGAACC 
CTGGACTTCT GTCTTTCAGG CCGGGGTACC CCAAAAGGCA AGACTGGTCG TGGGGGGCGC 
CAGAAGAACC GCTTTGGGCT CCCTGGACCC AGCCAGACGA ATCTCCCCAA CAGCCAGGCC 
AGCCAGGATG TGGCATCGCA GCCCTTCTCC CAGGGCGCCC TGACACAGGG CTACATCTCC 
ATGAGCCAGC CCTCCCAGAT GAGCCAGCCT GGCCTCTCCC AGCCCGAGTT GTCCCAGGAC 
AGTTACCTTG GCGATGAGTT TAAGTCACAG ATTGATGTGG CACTGTCGCA AGACTCCACG 
TACCAAGGGG AGCGGGCATA CCAGCACGGC GGAGTGACAG GGCTGTCCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_022406838.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022262546.1 Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X3, mRNA.

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CloneID	OCe295501
Clone ID Related Accession (Same CDS sequence)	XM_022406838.1
Accession Version	XM_022406838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	regulator of nonsense transcripts 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG ACATGCGGCT CATGCAGGGG 
GATGAGATAT GCCTGAGGTA CAAAGGGGAT CTGGCACCCC TCTGGAAAGG GATCGGCCAC 
GTCATCAAGG TCCCTGATAA TTATGGCGAT GAGATCGCCA TTGAGCTTCG GAGCAGTGTG 
GGTGCACCTG TGGAGGTGAC TCATAACTTC CAGGTGGATT TCGTATGGAA GTCAACCTCC 
TTTGATAGGA TGCAGAGTGC GTTGAAAACG TTCGCCGTGG ATGAGACCTC TGTGTCGGGC 
TACATCTACC ACAAACTGCT CGGGCATGAG GTGGAGGATG TGATCATTAA GTGCCAGCTG 
CCAAAACGCT TTACGGCACA GGGGCTCCCC GACCTCAACC ATTCTCAGGT TTATGCTGTG 
AAGACTGTGC TGCAAAGACC GCTGAGCCTG ATCCAGGGAC CACCAGGCAC AGGGAAGACA 
GTGACCTCTG CCACCATCGT CTATCACCTT GCTCGGCAGG GCAATGGGCC TGTGCTCGTC 
TGTGCTCCGA GTAACATAGC TGTGGATCAG CTGACTGAGA AGATCCACCA GACTGGGCTG 
AAAGTTGTAC GGCTTTGTGC CAAGAGCCGT GAGGCCATCG ACTCCCCAGT GTCATTCTTG 
GCCCTGCACA ACCAGATCAG GAACATGGAC AGCATGCCAG AGCTACAGAA GCTGCAGCAG 
CTGAAGGATG AGACGGGGGA GCTGTCATCT GCTGATGAGA AGCGGTACCG GGCTCTGAAG 
CGTACCGCTG AGAGGGAGCT GCTCATGAAT GCAGATGTCA TCTGCTGCAC ATGTGTGGGT 
GCAGGGGACC CAAGGCTTGC CAAGATGCAG TTCCGCTCCA TTTTAATTGA CGAGAGCACC 
CAGGCCACTG AGCCAGAATG CATGGTCCCT GTGGTCCTTG GTGCCAAGCA GCTCATCCTC 
GTGGGTGACC ACTGCCAGCT GGGCCCTGTG GTGATGTGCA AGAAAGCAGC CAAGGCTGGA 
CTATCTCAGT CGCTCTTTGA GCGCCTGGTG GTGCTGGGCA TCCGTCCCAT CCGCCTCCAG 
GTCCAGTATC GGATGCACCC TGCGCTCAGT GCCTTCCCAT CCAACATATT CTACGAGGGC 
TCTCTTCAGA ATGGCGTCAC TGCAGCTGAT CGAGTGAAGA AGGGATTTGA CTTCCAGTGG 
CCCCAACCTG ATAAGCCGAT GTTCTTCTAC GTGACCCAGG GCCAAGAGGA GATTGCTAGC 
TCAGGCACCT CCTACCTGAA CAGGACGGAA GCTGCAAATG TGGAGAAGAT CACCACGAAG 
CTGCTAAAGG CGGGTGCCAA GCCTGACCAG ATTGGCATCA TCACACCTTA CGAGGGCCAG 
CGCTCCTACC TGGTGCAGTA CATGCAGTTC AGCGGCTCTC TGCACACCAA GCTCTACCAG 
GAGGTAGAGA TTGCCAGCGT GGACGCATTC CAGGGGCGAG AGAAGGACTT CATCATTCTT 
TCCTGCGTGC GAGCCAACGA GCACCAAGGC ATTGGATTTT TAAACGACCC CAGGCGGCTT 
AATGTGGCCC TGACCAGAGC AAGGTATGGG GTTATTATTG TGGGCAACCC AAAGGCCCTG 
TCCAAGCAGC CCCTCTGGAA CCACCTGCTG AACTATTACA AGGAGCAAAA GGTGCTGGTG 
GAGGGGCCCC TGAACAACCT GCGGGAGAGC CTTATGCAGT TTAGCAAGCC CAGGAAGCTG 
GTCAATACCA TCAACCCGGG AGCCCGCTTC ATGACGACAG CCATGTATGA TGCCCGGGAG 
GCTATTATCC CAGGGTCAGT CTATGATCGG AGCAGCCAGG GCCGGCCCTC AAACATGTAC 
TTCCAGACCC ACGACCAGAT CGGCATGATT AGTGCAGGCC CCAGCCACGT GGCTGCCATG 
AACATTCCCA TCCCCTTCAA CCTGGTCATG CCCCCCATGC CACCACCAGG ATATTTTGGA 
CAAGCAAATG GGCCAGCCGC AGGAACCCTG GACTTCTGTC TTTCAGGCCG GGGTACCCCA 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTCCC TGGACCCAGC 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CATCGCAGCC CTTCTCCCAG 
GGCGCCCTGA CACAGGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCTGGC 
CTCTCCCAGC CCGAGTTGTC CCAGGACAGT TACCTTGGCG ATGAGTTTAA GTCACAGATT 
GATGTGGCAC TGTCGCAAGA CTCCACGTAC CAAGGGGAGC GGGCATACCA GCACGGCGGA 
GTGACAGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022262546.1
CDS	213..3596
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPVGAGAGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGTLDFCLSGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_022406838.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris UPF1, RNA helicase and ATPase (UPF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022406838.1

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGTTCT CAGACGCTCA CCTTCCTGGA CACCGAGGAA 
GCCGAGCTGC TCGGCGCCGA CACACAGGGC TCGGAGTTCG AGTTCACCGA CTTCACCCTC 
CCCAGTCAGA CGCAGACGCC CCCGGGTGGC CCCGGCGGCC CCGGAGGAGG TGGCGCGGGC 
GGCCCGGTCG GCGCTGGCGC GGGCGCTGCG GCCGGACAGC TGGACGCGCA GGTCGGTGGA 
CCAGAGGGCA TTCTGCAGAA TGGGGCCGTG GATGACAGCG TGGCCAAGAC CAGCCAGTTG 
TTGGCTGAGT TGAACTTTGA AGAAGATGAA GAAGATACCT ATTACACAAA GGATCTCCCT 
GTACATGCCT GCAGTTACTG CGGAATACAC GATCCTGCTT GCGTGGTTTA CTGTAACACC 
AGCAAGAAGT GGTTCTGTAA CGGGCGTGGA AATACTTCTG GCAGCCACAT TGTAAATCAC 
CTCGTGAGGG CAAAATGCAA AGAGGTGACT CTGCACAAGG ATGGGCCCCT GGGGGAGACA 
GTCCTGGAAT GTTACAACTG TGGCTGCCGC AATGTCTTCC TCCTTGGCTT CATCCCTGCC 
AAGGCTGACT CAGTAGTGGT GCTGCTGTGC AGGCAGCCCT GCGCCAGTCA GAGCAGCCTG 
AAGGACATCA ACTGGGATAG CTCCCAGTGG CAGCCCCTCA TCCAGGACCG CTGCTTCCTC 
TCTTGGCTGG TCAAGATCCC TTCTGAGCAG GAGCAGCTGC GGGCTCGGCA GATCACCGCC 
CAGCAGATCA ACAAGCTGGA GGAGCTCTGG AAGGAAAATC CTTCTGCCAC TTTGGAGGAC 
CTGGAGAAGC CAGGCGTGGA CGAGGAGCCG CAGCATGTCC TCCTGCGGTA CGAGGATGCC 
TACCAGTACC AGAACATATT CGGCCCTCTG GTCAAGCTGG AGGCCGACTA CGATAAGAAA 
CTGAAAGAGT CCCAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG 
AAGAGAATCG CCTACTTCAC TTTGCCCAAG ACTGACTCTG ACATGCGGCT CATGCAGGGG 
GATGAGATAT GCCTGAGGTA CAAAGGGGAT CTGGCACCCC TCTGGAAAGG GATCGGCCAC 
GTCATCAAGG TCCCTGATAA TTATGGCGAT GAGATCGCCA TTGAGCTTCG GAGCAGTGTG 
GGTGCACCTG TGGAGGTGAC TCATAACTTC CAGGTGGATT TCGTATGGAA GTCAACCTCC 
TTTGATAGGA TGCAGAGTGC GTTGAAAACG TTCGCCGTGG ATGAGACCTC TGTGTCGGGC 
TACATCTACC ACAAACTGCT CGGGCATGAG GTGGAGGATG TGATCATTAA GTGCCAGCTG 
CCAAAACGCT TTACGGCACA GGGGCTCCCC GACCTCAACC ATTCTCAGGT TTATGCTGTG 
AAGACTGTGC TGCAAAGACC GCTGAGCCTG ATCCAGGGAC CACCAGGCAC AGGGAAGACA 
GTGACCTCTG CCACCATCGT CTATCACCTT GCTCGGCAGG GCAATGGGCC TGTGCTCGTC 
TGTGCTCCGA GTAACATAGC TGTGGATCAG CTGACTGAGA AGATCCACCA GACTGGGCTG 
AAAGTTGTAC GGCTTTGTGC CAAGAGCCGT GAGGCCATCG ACTCCCCAGT GTCATTCTTG 
GCCCTGCACA ACCAGATCAG GAACATGGAC AGCATGCCAG AGCTACAGAA GCTGCAGCAG 
CTGAAGGATG AGACGGGGGA GCTGTCATCT GCTGATGAGA AGCGGTACCG GGCTCTGAAG 
CGTACCGCTG AGAGGGAGCT GCTCATGAAT GCAGATGTCA TCTGCTGCAC ATGTGTGGGT 
GCAGGGGACC CAAGGCTTGC CAAGATGCAG TTCCGCTCCA TTTTAATTGA CGAGAGCACC 
CAGGCCACTG AGCCAGAATG CATGGTCCCT GTGGTCCTTG GTGCCAAGCA GCTCATCCTC 
GTGGGTGACC ACTGCCAGCT GGGCCCTGTG GTGATGTGCA AGAAAGCAGC CAAGGCTGGA 
CTATCTCAGT CGCTCTTTGA GCGCCTGGTG GTGCTGGGCA TCCGTCCCAT CCGCCTCCAG 
GTCCAGTATC GGATGCACCC TGCGCTCAGT GCCTTCCCAT CCAACATATT CTACGAGGGC 
TCTCTTCAGA ATGGCGTCAC TGCAGCTGAT CGAGTGAAGA AGGGATTTGA CTTCCAGTGG 
CCCCAACCTG ATAAGCCGAT GTTCTTCTAC GTGACCCAGG GCCAAGAGGA GATTGCTAGC 
TCAGGCACCT CCTACCTGAA CAGGACGGAA GCTGCAAATG TGGAGAAGAT CACCACGAAG 
CTGCTAAAGG CGGGTGCCAA GCCTGACCAG ATTGGCATCA TCACACCTTA CGAGGGCCAG 
CGCTCCTACC TGGTGCAGTA CATGCAGTTC AGCGGCTCTC TGCACACCAA GCTCTACCAG 
GAGGTAGAGA TTGCCAGCGT GGACGCATTC CAGGGGCGAG AGAAGGACTT CATCATTCTT 
TCCTGCGTGC GAGCCAACGA GCACCAAGGC ATTGGATTTT TAAACGACCC CAGGCGGCTT 
AATGTGGCCC TGACCAGAGC AAGGTATGGG GTTATTATTG TGGGCAACCC AAAGGCCCTG 
TCCAAGCAGC CCCTCTGGAA CCACCTGCTG AACTATTACA AGGAGCAAAA GGTGCTGGTG 
GAGGGGCCCC TGAACAACCT GCGGGAGAGC CTTATGCAGT TTAGCAAGCC CAGGAAGCTG 
GTCAATACCA TCAACCCGGG AGCCCGCTTC ATGACGACAG CCATGTATGA TGCCCGGGAG 
GCTATTATCC CAGGGTCAGT CTATGATCGG AGCAGCCAGG GCCGGCCCTC AAACATGTAC 
TTCCAGACCC ACGACCAGAT CGGCATGATT AGTGCAGGCC CCAGCCACGT GGCTGCCATG 
AACATTCCCA TCCCCTTCAA CCTGGTCATG CCCCCCATGC CACCACCAGG ATATTTTGGA 
CAAGCAAATG GGCCAGCCGC AGGAACCCTG GACTTCTGTC TTTCAGGCCG GGGTACCCCA 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTCCC TGGACCCAGC 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CATCGCAGCC CTTCTCCCAG 
GGCGCCCTGA CACAGGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCTGGC 
CTCTCCCAGC CCGAGTTGTC CCAGGACAGT TACCTTGGCG ATGAGTTTAA GTCACAGATT 
GATGTGGCAC TGTCGCAAGA CTCCACGTAC CAAGGGGAGC GGGCATACCA GCACGGCGGA 
GTGACAGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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UPF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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