cdk2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdk2
Entrez Gene ID	406715
Full Name	cyclin-dependent kinase 2
Synonyms	wu:fa10c02,zgc:56598,zgc:77684
General protein information	Preferred Names cyclin-dependent kinase 2 Names cyclin-dependent kinase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213406.1	NP_998571.1	cyclin-dependent kinase 2

Pathways from BioSystems

Homologies

Neurospora crassa	NCU09778	XP_960117.1
Pan troglodytes (chimpanzee)	CDK2	XP_003952210.1
Oryza sativa (rice)	Os03g0118400	NP_001048772.1
Saccharomyces cerevisiae (baker's yeast)	CDC28	NP_009718.3
Schizosaccharomyces pombe (fission yeast)	cdc2	NP_595629.1
Eremothecium gossypii	AGOS_ADR058C	NP_984154.1
Kluyveromyces lactis	KLLA0B09790g	XP_451964.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004579	XP_562342.4
Bos taurus (cattle)	CDK2	NP_001014934.1
Mus musculus (house mouse)	Cdk2	NP_904326.1
Rattus norvegicus (Norway rat)	Cdk2	NP_955795.1
Drosophila melanogaster (fruit fly)	cdc2c	NP_732544.1
Arabidopsis thaliana (thale cress)	CDC2	NP_566911.1
Xenopus tropicalis (tropical clawed frog)	cdk2	NP_001008136.1
Magnaporthe oryzae (rice blast fungus)	MGG_01362	XP_003714294.1
Homo sapiens (human)	CDK2	NP_001789.2
Macaca mulatta (Rhesus monkey)	CDK2	XP_001113345.1
Canis lupus familiaris (dog)	CDK2	XP_005625536.1
Gallus gallus (chicken)	CDK2	NP_001186786.1
Oryza sativa (rice)	Os02g0123100	NP_001045731.1
Danio rerio (zebrafish)	cdk2	NP_998571.1

Gene Ontology
Bibliography

Related articles in PubMed

Reversion of tumor hepatocytes to normal hepatocytes during liver tumor regression in an oncogene-expressing transgenic zebrafish model.
Li Y, Agrawal I, Gong Z
Disease models & mechanisms12(10)(2019 10)

A Novel AURKA Mutant-Induced Early-Onset Severe Hepatocarcinogenesis Greater than Wild-Type via Activating Different Pathways in Zebrafish.
Su ZL, Su CW, Huang YL, Yang WY, Sampurna BP, Ouchi T, Lee KL, Wu CS, Wang HD, Yuh CH
Cancers11(7)(2019 Jul)

Ribose-5-phosphate isomerase A overexpression promotes liver cancer development in transgenic zebrafish via activation of ERK and β-catenin pathways.
Chou YT, Chen LY, Tsai SL, Tu HC, Lu JW, Ciou SC, Wang HD, Yuh CH
Carcinogenesis40(3)461-473(2019 05)

Identification of Novel Anti-Liver Cancer Small Molecules with Better Therapeutic Index than Sorafenib via Zebrafish Drug Screening Platform.
Lin HS, Huang YL, Wang YS, Hsiao E, Hsu TA, Shiao HY, Jiaang WT, Sampurna BP, Lin KH, Wu MS, Lai GM, Yuh CH
Cancers11(6)(2019 May)

Up-regulation of golgi α-mannosidase IA and down-regulation of golgi α-mannosidase IC activates unfolded protein response during hepatocarcinogenesis.
Tu HC, Hsiao YC, Yang WY, Tsai SL, Lin HK, Liao CY, Lu JW, Chou YT, Wang HD, Yuh CH
Hepatology communications1(3)230-247(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cdk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01944	NM_213406.1 Latest version!	Danio rerio cyclin-dependent kinase 2 (cdk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdk2 ( NM_213406.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998571.1 Danio rerio cyclin-dependent kinase 2 (cdk2), mRNA.

Price & Availability↑

CloneID	ODa01944
Clone ID Related Accession (Same CDS sequence)	NM_213406.1
Accession Version	NM_213406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	cyclin-dependent kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC049499.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049499.1, BC062836.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476760 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCTT TTCAGAAAGT GGAGAAGATC GGAGAGGGAA CATACGGAGT GGTTTATAAA 
GCCAAAAATA AAGTTACAGG AGAAACTGTC GCGCTGAAGA AAATCCGATT AGATACGGAA 
ACTGAAGGTG TTCCCAGCAC TGCCATTCGT GAGATCTCTC TGCTAAAAGA GCTCAATCAC 
CCTAACATAG TCAAGTTGCG TGATGTGATA CACACTGAAA ATAAACTTTA CTTGGTGTTT 
GAGTTTCTTC ACCAAGACCT GAAGAGGTTT ATGGACTCGA CGTCTGTCAG TGGCATATCC 
TTGCCACTCG TGAAGAGTTA CCTATTCCAG CTGCTCCAGG GATTGGCCTT CTGTCACTCC 
CATCGGGTTC TTCACAGAGA TCTTAAACCC CAGAATCTCC TCATCAACGC TCAGGGCGAG 
ATCAAACTGG CTGACTTTGG TTTGGCCAGA GCGTTCGGTG TACCTGTGCG GACTTATACA 
CACGAGGTTG TAACTTTGTG GTACAGAGCT CCAGAGATTC TCCTGGGATG TAAATATTAT 
TCTACAGCTG TTGACATCTG GAGTTTGGGC TGCATCTTTG CTGAAATGAT TACTCGGAGG 
GCACTGTTTC CTGGAGACTC TGAAATAGAT CAGCTCTTCC GGATATTTCG AACACTGGGC 
ACTCCTGATG AGTCTATATG GCCTGGAGTT ACATCAATGC CAGACTACAA ACCCTCCTTT 
CCCAAATGGG CTCGACAGGA CCTGTCTAAA GTGGTGCCCC CCCTTGATGA AGATGGCAGA 
GACCTTCTTG GGCAAATGTT GACCTATGAT CCAAACAAGA GGATCTCGGC AAAGAACGCT 
CTTGTTCATC GATTTTTCCG CGACGTCACC ATGCCAGTGC CTCCTTTACG CCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998571.1
CDS	164..1060
Translation	MESFQKVEKIGEGTYGVVYKAKNKVTGETVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLRDVIHTENKLYLVFEFLHQDLKRFMDSTSVSGISLPLVKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINAQGEIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMITRRALFPGDSEIDQLFRIFRTLGTPDESIWPGVTSMPDYKPSFPKWARQDLSKVVPPLDEDGRDLLGQMLTYDPNKRISAKNALVHRFFRDVTMPVPPLRL

Target ORF information:

RefSeq Version	NM_213406.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cyclin-dependent kinase 2 (cdk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213406.1

ORF Insert Sequence:

ATGGAGTCTT TTCAGAAAGT GGAGAAGATC GGAGAGGGAA CATACGGAGT GGTTTATAAA 
GCCAAAAATA AAGTTACAGG AGAAACTGTC GCGCTGAAGA AAATCCGATT AGATACGGAA 
ACTGAAGGTG TTCCCAGCAC TGCCATTCGT GAGATCTCTC TGCTAAAAGA GCTCAATCAC 
CCTAACATAG TCAAGTTGCG TGATGTGATA CACACTGAAA ATAAACTTTA CTTGGTGTTT 
GAGTTTCTTC ACCAAGACCT GAAGAGGTTT ATGGACTCGA CGTCTGTCAG TGGCATATCC 
TTGCCACTCG TGAAGAGTTA CCTATTCCAG CTGCTCCAGG GATTGGCCTT CTGTCACTCC 
CATCGGGTTC TTCACAGAGA TCTTAAACCC CAGAATCTCC TCATCAACGC TCAGGGCGAG 
ATCAAACTGG CTGACTTTGG TTTGGCCAGA GCGTTCGGTG TACCTGTGCG GACTTATACA 
CACGAGGTTG TAACTTTGTG GTACAGAGCT CCAGAGATTC TCCTGGGATG TAAATATTAT 
TCTACAGCTG TTGACATCTG GAGTTTGGGC TGCATCTTTG CTGAAATGAT TACTCGGAGG 
GCACTGTTTC CTGGAGACTC TGAAATAGAT CAGCTCTTCC GGATATTTCG AACACTGGGC 
ACTCCTGATG AGTCTATATG GCCTGGAGTT ACATCAATGC CAGACTACAA ACCCTCCTTT 
CCCAAATGGG CTCGACAGGA CCTGTCTAAA GTGGTGCCCC CCCTTGATGA AGATGGCAGA 
GACCTTCTTG GGCAAATGTT GACCTATGAT CCAAACAAGA GGATCTCGGC AAAGAACGCT 
CTTGTTCATC GATTTTTCCG CGACGTCACC ATGCCAGTGC CTCCTTTACG CCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reversion of tumor hepatocytes to normal hepatocytes during liver tumor regression in an oncogene-expressing transgenic zebrafish model.
Disease models & mechanisms12(10)(2019 10)
Li Y,Agrawal I,Gong Z

A Novel AURKA Mutant-Induced Early-Onset Severe Hepatocarcinogenesis Greater than Wild-Type via Activating Different Pathways in Zebrafish.
Cancers11(7)(2019 Jul)
Su ZL,Su CW,Huang YL,Yang WY,Sampurna BP,Ouchi T,Lee KL,Wu CS,Wang HD,Yuh CH

Ribose-5-phosphate isomerase A overexpression promotes liver cancer development in transgenic zebrafish via activation of ERK and β-catenin pathways.
Carcinogenesis40(3)461-473(2019 05)
Chou YT,Chen LY,Tsai SL,Tu HC,Lu JW,Ciou SC,Wang HD,Yuh CH

Identification of Novel Anti-Liver Cancer Small Molecules with Better Therapeutic Index than Sorafenib via Zebrafish Drug Screening Platform.
Cancers11(6)(2019 May)
Lin HS,Huang YL,Wang YS,Hsiao E,Hsu TA,Shiao HY,Jiaang WT,Sampurna BP,Lin KH,Wu MS,Lai GM,Yuh CH

Up-regulation of golgi α-mannosidase IA and down-regulation of golgi α-mannosidase IC activates unfolded protein response during hepatocarcinogenesis.
Hepatology communications1(3)230-247(2017 05)
Tu HC,Hsiao YC,Yang WY,Tsai SL,Lin HK,Liao CY,Lu JW,Chou YT,Wang HD,Yuh CH