piwil1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	piwil1
Entrez Gene ID	368200
Full Name	piwi-like RNA-mediated gene silencing 1
Synonyms	ziwi
General protein information	Preferred Names piwi-like RNA-mediated gene silencing 1 Names piwi-like protein 1 piwi-like 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_183338.1	NP_899181.1	piwi-like protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIWIL1	NP_004755.2
Pan troglodytes (chimpanzee)	PIWIL1	XP_001137913.1
Xenopus tropicalis (tropical clawed frog)	LOC100496286	XP_002942570.2
Canis lupus familiaris (dog)	PIWIL1	XP_534638.2
Mus musculus (house mouse)	Piwil1	NP_067286.1
Rattus norvegicus (Norway rat)	Piwil1	NP_001102323.1
Danio rerio (zebrafish)	piwil1	NP_899181.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011204	XP_564296.2
Caenorhabditis elegans (roundworm)	prg-1	NP_492121.1
Caenorhabditis elegans (roundworm)	prg-2	NP_500994.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009509	XP_310187.3
Bos taurus (cattle)	PIWIL1	XP_002694505.2
Gallus gallus (chicken)	PIWIL1	NP_001092322.1
Drosophila melanogaster (fruit fly)	aub	NP_476734.1
Xenopus tropicalis (tropical clawed frog)	piwil1	XP_002940227.2

Gene Ontology
Bibliography

Related articles in PubMed

Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline.
Ye D, Zhu L, Zhang Q, Xiong F, Wang H, Wang X, He M, Zhu Z, Sun Y
Marine biotechnology (New York, N.Y.)21(2)217-228(2019 Apr)

Germline Stem Cells Drive Ovary Regeneration in Zebrafish.
Cao Z, Mao X, Luo L
Cell reports26(7)1709-1717.e3(2019 02)

Functional equivalence of germ plasm organizers.
Krishnakumar P, Riemer S, Perera R, Lingner T, Goloborodko A, Khalifa H, Bontems F, Kaufholz F, El-Brolosy MA, Dosch R
PLoS genetics14(11)e1007696(2018 11)

The protective effects of selenium-enriched spirulina on the reproductive system of male zebrafish (Danio rerio) exposed to beta-cypermethrin.
Zhang Y , Zhou Y , Tang Q , Hu F , Feng L , Shen J , Huang B
Food & function9(11)5791-5804(2018 Nov)

A draft genome of the striped catfish, Pangasianodon hypophthalmus, for comparative analysis of genes relevant to development and a resource for aquaculture improvement.
Kim OTP, Nguyen PT, Shoguchi E, Hisata K, Vo TTB, Inoue J, Shinzato C, Le BTN, Nishitsuji K, Kanda M, Nguyen VH, Nong HV, Satoh N
BMC genomics19(1)733(2018 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following piwil1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the piwil1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02953	NM_183338.1 Latest version!	Danio rerio piwi-like RNA-mediated gene silencing 1 (piwil1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

piwil1 ( NM_183338.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_899181.1 Danio rerio piwi-like RNA-mediated gene silencing 1 (piwil1), mRNA.

Price & Availability↑

CloneID	ODa02953
Clone ID Related Accession (Same CDS sequence)	NM_183338.1
Accession Version	NM_183338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Danio rerio(zebrafish)
Product	piwi-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF336369.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF336369.1 [ECO:0000332] ##Evidence-Data-END##

ATGACAGGAC GAGCAAGAGC AAGATCGAGG GGCAGAGGTC GTGGTCAGGA GCCTGCTGCT 
CCTGGCGCAC AACCACCTGT ATCTCAAGAG GCTGCAAAGC CTGTTGTGTC CACTCCGTCT 
GAAGGACAAC TGGTGGGCAG AGGGAGACAG AAACCTGCAC CTGGAGCCAT GTCTGAAGAA 
GCGATGCTCC AGATTTCTGC TGGCTTTCAG CAGGTGAAGA TCGGGGAGAG AGGTGGACGC 
AGACGGGATT TCCACGATTC TGGTGTCCAT ACTCGTCAAC TTATGGAACA CGTGAAAGAA 
TCCAAAACAG GTGTTTCTGG GACTGCCATT GAGCTGAGGG CCAATTTTAT GCGTCTGCTT 
TCACGTCCTA TGTGGGCTTT GTATCAGTAT CATGTGGACT ACAAGCCACC AATGGAGTCC 
AGACGACTGC GTTCGGCTTT GCTTTTCCAG CACGAGGAGA CTCTGGGGAA AGCACACACC 
TTTGATGGAG CCATTCTCTT TCTCCCCAAC AAACTGCGCA ACGCTGAGAC AGTCCTGTGC 
AGTGAGACCA GAAATGGAGA GAAGGTTGAG ATCACTGTGA CACTGACCAA TGAGCTGCCG 
CCGTCCTCTC CTGTATGCCT GCAGTTTTAC AACATCCTCT TCAGGAGAAT CCTAAGAATA 
CTAAACATGC AGCAGATCGG ACGTCATTAC TACAATCCTG ATGACCCATT CAACATCCCA 
CAGCACAGGC TGACAATTTG GCCTGGCTTC ATGACCACCA TCCTACAGTA CGAGTCCAGC 
ATCATGCTGT GCTCTGATGT GAGCCACAAG GTTCTGCGCA GTGAGACGGT TCTTGACTTT 
ATGTACAGTC TGAGACAGCA GTGTGGAGAC CAGCGCTTCC CTGAAGCCTG CACGAAGGAG 
CTTGTGGGCC TGATCATTCT GACCAAGTAC AACAACAAGA CATACAGGAT TGATGACATT 
GCCTGGGATC ATACTCCCAA CAACACATTC AAGAAAGGAG ATACAGAAAT TTCATTCAAG 
AACTACTTCA AATCGCAATA TGGTCTTGAC ATAACAGATG GCAACCAGGT TCTTCTCGTT 
AGCCATGTGA AGAGACTTGG CCCCTCAGGA CGCCCTCCAC CGGGACCAGC TATGCTTGTC 
CCCGAGTTCT GCTACCTCAC TGGTCTAACC GATAAAATGC GTGCTGATTT TAACATCATG 
AAGGATCTTG CTTCACATAC ACGGCTAAGT CCTGAACAGA GAGAGGGCAG AATTAACCGC 
TTAATCTCCA ACATCAACAG AAATGGAGAC GTGCAGAATG AACTGACTAC TTGGGGTCTG 
AGCTTTGAGA ACAAGCTTCT TAGTCTTAAT GGGAGAGTTT TGCCCTCTGA GAGGATCATA 
CAGGGAGGCA GAGCATTTGA GTATAACCCA TGGACAGCAG ACTGGTCTAA AGAGATGAGA 
GGTCTCCCTC TGATTAGCTG CATGTCTCTG GACAACTGGC TCATGTTCTA CACGCGCAGA 
AATGCAGATG TTGCCCAATC TCTGCTTCAG ACTCTCAACA AAGTGTCTGG ACCCATGGGG 
ATCCGCATGC AGAGGGCTGT CATGATTGAG TATGAAGATC GTCAGGAATC TCTCCTTAGA 
GCTTTGCAAC AAAACGTAGC ACGTGAAACT CAGATGGTGG TGGTGATCTT GCCTACCAAT 
CGAAAAGACA AGTACGACTG TGTAAAGAAG TACCTGTGTG TGGACTGCCC TACTCCTAGT 
CAGTGTGTGG TGTCTCGCAC CATCAGCAAA CCTCAAGCAC TTATGACTGT GGCCACCAAG 
ATTGCTTTGC AGATGAACTG CAAAATGGGA GGAGAGCTGT GGAGTGTAGA AATTCCACTC 
CGGCAGCTCA TGATTGTAGG TATAGACTGC TACCATGATA CCGCTGCTGG AAAAAGGTCC 
ATCGGCGCAA TGGTGGCCAG TCTAAATCAG GGCATGTCAA GGTGGTTCTC CAAGTGTGTC 
TTGCAAAACC GTGGACAGGA GATCATCGAT GCACTGAAGG GGTCATTGCA GGGTGCTCTG 
AAGGCCTATC TGAAATACAA CAATTCTCTG CCCTCGCGCA TCATTGTTTA CCGAGATGGA 
GTTGGAGATG GCATGCTGCA GAGCGTTGTG GACTATGAAG TTCCTCAGAT CATGCAGTCT 
ATCAAGACCA TGGGGCAAGA TTATGAGCCC AAGCTCTCAG TAGTGGTGGT GAAAAAGCGC 
ATCAGCTCCC GATTCTTTGC TCGGATTGAT GGCAAAATTG CCAATCCCCC TCCTGGGACA 
GTCATTGACA CTGAAGTCAC TCGTCCAGAG TGGTATGATT TCTTCATTGT AAGCCAGGCT 
GTGCGCTTTG GGTGTGTCGC ACCTACTCAC TACAATGTGG TGTTTGACAA CAGCGGCCTT 
AAACCAGATC ACATGCAGAG ACTCACCTAC AAACTTTGCC ACATGTACTA CAACTGGCAG 
GGGATTGTGC GAGTGCCAGC TCCATGCCAG TATGCCCACA AATTGGCCTT CCTGGTTGGC 
CAGAGCATTC ACAAGGAGCC TAACATGAAT CTGGATGACT TCCTGTATTA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_899181.1
CDS	64..2640
Translation	MTGRARARSRGRGRGQEPAAPGAQPPVSQEAAKPVVSTPSEGQLVGRGRQKPAPGAMSEEAMLQISAGFQQVKIGERGGRRRDFHDSGVHTRQLMEHVKESKTGVSGTAIELRANFMRLLSRPMWALYQYHVDYKPPMESRRLRSALLFQHEETLGKAHTFDGAILFLPNKLRNAETVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDDPFNIPQHRLTIWPGFMTTILQYESSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQRFPEACTKELVGLIILTKYNNKTYRIDDIAWDHTPNNTFKKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVKRLGPSGRPPPGPAMLVPEFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQNELTTWGLSFENKLLSLNGRVLPSERIIQGGRAFEYNPWTADWSKEMRGLPLISCMSLDNWLMFYTRRNADVAQSLLQTLNKVSGPMGIRMQRAVMIEYEDRQESLLRALQQNVARETQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDNSGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHKEPNMNLDDFLYYL

Target ORF information:

RefSeq Version	NM_183338.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio piwi-like RNA-mediated gene silencing 1 (piwil1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183338.1

ORF Insert Sequence:

ATGACAGGAC GAGCAAGAGC AAGATCGAGG GGCAGAGGTC GTGGTCAGGA GCCTGCTGCT 
CCTGGCGCAC AACCACCTGT ATCTCAAGAG GCTGCAAAGC CTGTTGTGTC CACTCCGTCT 
GAAGGACAAC TGGTGGGCAG AGGGAGACAG AAACCTGCAC CTGGAGCCAT GTCTGAAGAA 
GCGATGCTCC AGATTTCTGC TGGCTTTCAG CAGGTGAAGA TCGGGGAGAG AGGTGGACGC 
AGACGGGATT TCCACGATTC TGGTGTCCAT ACTCGTCAAC TTATGGAACA CGTGAAAGAA 
TCCAAAACAG GTGTTTCTGG GACTGCCATT GAGCTGAGGG CCAATTTTAT GCGTCTGCTT 
TCACGTCCTA TGTGGGCTTT GTATCAGTAT CATGTGGACT ACAAGCCACC AATGGAGTCC 
AGACGACTGC GTTCGGCTTT GCTTTTCCAG CACGAGGAGA CTCTGGGGAA AGCACACACC 
TTTGATGGAG CCATTCTCTT TCTCCCCAAC AAACTGCGCA ACGCTGAGAC AGTCCTGTGC 
AGTGAGACCA GAAATGGAGA GAAGGTTGAG ATCACTGTGA CACTGACCAA TGAGCTGCCG 
CCGTCCTCTC CTGTATGCCT GCAGTTTTAC AACATCCTCT TCAGGAGAAT CCTAAGAATA 
CTAAACATGC AGCAGATCGG ACGTCATTAC TACAATCCTG ATGACCCATT CAACATCCCA 
CAGCACAGGC TGACAATTTG GCCTGGCTTC ATGACCACCA TCCTACAGTA CGAGTCCAGC 
ATCATGCTGT GCTCTGATGT GAGCCACAAG GTTCTGCGCA GTGAGACGGT TCTTGACTTT 
ATGTACAGTC TGAGACAGCA GTGTGGAGAC CAGCGCTTCC CTGAAGCCTG CACGAAGGAG 
CTTGTGGGCC TGATCATTCT GACCAAGTAC AACAACAAGA CATACAGGAT TGATGACATT 
GCCTGGGATC ATACTCCCAA CAACACATTC AAGAAAGGAG ATACAGAAAT TTCATTCAAG 
AACTACTTCA AATCGCAATA TGGTCTTGAC ATAACAGATG GCAACCAGGT TCTTCTCGTT 
AGCCATGTGA AGAGACTTGG CCCCTCAGGA CGCCCTCCAC CGGGACCAGC TATGCTTGTC 
CCCGAGTTCT GCTACCTCAC TGGTCTAACC GATAAAATGC GTGCTGATTT TAACATCATG 
AAGGATCTTG CTTCACATAC ACGGCTAAGT CCTGAACAGA GAGAGGGCAG AATTAACCGC 
TTAATCTCCA ACATCAACAG AAATGGAGAC GTGCAGAATG AACTGACTAC TTGGGGTCTG 
AGCTTTGAGA ACAAGCTTCT TAGTCTTAAT GGGAGAGTTT TGCCCTCTGA GAGGATCATA 
CAGGGAGGCA GAGCATTTGA GTATAACCCA TGGACAGCAG ACTGGTCTAA AGAGATGAGA 
GGTCTCCCTC TGATTAGCTG CATGTCTCTG GACAACTGGC TCATGTTCTA CACGCGCAGA 
AATGCAGATG TTGCCCAATC TCTGCTTCAG ACTCTCAACA AAGTGTCTGG ACCCATGGGG 
ATCCGCATGC AGAGGGCTGT CATGATTGAG TATGAAGATC GTCAGGAATC TCTCCTTAGA 
GCTTTGCAAC AAAACGTAGC ACGTGAAACT CAGATGGTGG TGGTGATCTT GCCTACCAAT 
CGAAAAGACA AGTACGACTG TGTAAAGAAG TACCTGTGTG TGGACTGCCC TACTCCTAGT 
CAGTGTGTGG TGTCTCGCAC CATCAGCAAA CCTCAAGCAC TTATGACTGT GGCCACCAAG 
ATTGCTTTGC AGATGAACTG CAAAATGGGA GGAGAGCTGT GGAGTGTAGA AATTCCACTC 
CGGCAGCTCA TGATTGTAGG TATAGACTGC TACCATGATA CCGCTGCTGG AAAAAGGTCC 
ATCGGCGCAA TGGTGGCCAG TCTAAATCAG GGCATGTCAA GGTGGTTCTC CAAGTGTGTC 
TTGCAAAACC GTGGACAGGA GATCATCGAT GCACTGAAGG GGTCATTGCA GGGTGCTCTG 
AAGGCCTATC TGAAATACAA CAATTCTCTG CCCTCGCGCA TCATTGTTTA CCGAGATGGA 
GTTGGAGATG GCATGCTGCA GAGCGTTGTG GACTATGAAG TTCCTCAGAT CATGCAGTCT 
ATCAAGACCA TGGGGCAAGA TTATGAGCCC AAGCTCTCAG TAGTGGTGGT GAAAAAGCGC 
ATCAGCTCCC GATTCTTTGC TCGGATTGAT GGCAAAATTG CCAATCCCCC TCCTGGGACA 
GTCATTGACA CTGAAGTCAC TCGTCCAGAG TGGTATGATT TCTTCATTGT AAGCCAGGCT 
GTGCGCTTTG GGTGTGTCGC ACCTACTCAC TACAATGTGG TGTTTGACAA CAGCGGCCTT 
AAACCAGATC ACATGCAGAG ACTCACCTAC AAACTTTGCC ACATGTACTA CAACTGGCAG 
GGGATTGTGC GAGTGCCAGC TCCATGCCAG TATGCCCACA AATTGGCCTT CCTGGTTGGC 
CAGAGCATTC ACAAGGAGCC TAACATGAAT CTGGATGACT TCCTGTATTA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline.
Marine biotechnology (New York, N.Y.)21(2)217-228(2019 Apr)
Ye D,Zhu L,Zhang Q,Xiong F,Wang H,Wang X,He M,Zhu Z,Sun Y

Germline Stem Cells Drive Ovary Regeneration in Zebrafish.
Cell reports26(7)1709-1717.e3(2019 02)
Cao Z,Mao X,Luo L

Functional equivalence of germ plasm organizers.
PLoS genetics14(11)e1007696(2018 11)
Krishnakumar P,Riemer S,Perera R,Lingner T,Goloborodko A,Khalifa H,Bontems F,Kaufholz F,El-Brolosy MA,Dosch R

The protective effects of selenium-enriched spirulina on the reproductive system of male zebrafish (Danio rerio) exposed to beta-cypermethrin.
Food & function9(11)5791-5804(2018 Nov)
Zhang Y ,Zhou Y ,Tang Q ,Hu F ,Feng L ,Shen J ,Huang B

A draft genome of the striped catfish, Pangasianodon hypophthalmus, for comparative analysis of genes relevant to development and a resource for aquaculture improvement.
BMC genomics19(1)733(2018 Oct)
Kim OTP,Nguyen PT,Shoguchi E,Hisata K,Vo TTB,Inoue J,Shinzato C,Le BTN,Nishitsuji K,Kanda M,Nguyen VH,Nong HV,Satoh N

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piwil1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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