dspa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dspa
Entrez Gene ID	324023
Full Name	desmoplakin a
Synonyms	im:6911953,sb:cb570,wu:fc17a08,wu:fk73d01
General protein information	Preferred Names desmoplakin a Names desmoplakin a desmoplakin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001919866.7	XP_001919901.3	desmoplakin isoform X1
XM_001919866.6	XP_001919901.3	desmoplakin isoform X1
XM_005171104.3	XP_005171161.1	desmoplakin isoform X2
XM_005171104.4	XP_005171161.1	desmoplakin isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DSP	XP_518227.2
Canis lupus familiaris (dog)	DSP	XP_545329.3
Homo sapiens (human)	DSP	NP_004406.2
Bos taurus (cattle)	DSP	NP_001179297.1
Rattus norvegicus (Norway rat)	Dsp	XP_225259.4
Gallus gallus (chicken)	DSP	XP_418957.3
Macaca mulatta (Rhesus monkey)	DSP	XP_001085012.1
Mus musculus (house mouse)	Dsp	NP_076331.2
Danio rerio (zebrafish)	dspa	XP_001919901.3

Gene Ontology

The following dspa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dspa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa06595	XM_001919866.7 Latest version!	Danio rerio desmoplakin a (dspa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa06596	XM_005171104.4 Latest version!	Danio rerio desmoplakin a (dspa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODa06595	XM_001919866.6	Danio rerio desmoplakin a (dspa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa06596	XM_005171104.3	Danio rerio desmoplakin a (dspa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dspa ( XM_001919866.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001919901.3 Danio rerio desmoplakin a (dspa), transcript variant X1, mRNA.

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CloneID	ODa06595
Clone ID Related Accession (Same CDS sequence)	XM_001919866.7 , XM_001919866.6
Accession Version	XM_001919866.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	desmoplakin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919866.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGT CCGGTTCTCA GACCAGGCTC CACCAGATCA GCCGCAGGTC CAGTTCGCGG 
CCAGACCTCA CCGCCGTCGG CTACAGTTTA CCGAGGAACG ACGGAGCTCA GTTTTTCACG 
AGTGGAAACG GATACCAGTC CGACTATAAT GACGGCTACA TGCAGACCTA CACGCAGACT 
TTCTCCAAAA ACTCTCAGGG TGGAGGAGCT GGAGGAGCCC AGAGCATGTC TTCCATGGCG 
GTTCAGAAGC GAGCTCAGAT GTTGTACAGT GACTGCATGG GATTCCTGCA GCGAGCGCAG 
GGCCTCCTGG AGACTATGGG ACCTGCTTCT GAGGTGGACA AGAACATGAT GGCAGCGCAG 
GACGCCATGG ATCAGCTGCG GAAATGTGCG GTGGACATGA GGAACGCCGG TCTGCCTAAT 
GACATGATCC TCCGCAGTTT GGAGGACTGT CATAGCTTTT ATGCAGACAT TCGCAACTCC 
ATCACGGGCA CCACCATCAG GAGGACTGGC GGCACCGTCA GCGGCACCAT CGGTGGCACC 
ATCAGTGGCA CCATTGGTGG CACTGTCGGC GGCTGGGACG ATCCCAGCAA GAGCTTTCAG 
GACGCACTGT CATGGATCAA CCAGAAGAAG AGACTCATTG AAACGTCAGG CTTCGGTGAA 
AATTCAGAAG CCATTAGCCA ACAAATCCTG AATCACAACA AGTTCCACAG TTCAATCCAG 
AGAAGCCAGG AGGTGGACAG AGCCCGCGAC GATCTGATGC AGAGCAGGGA AAAGGCTGGA 
CTACATGCTC TGGACCAAGA GTGGGACAGT TTACAGAAAA TGTCCCATGC ACGTATGGAT 
CATCTGCGAG ACCTACAGAG CATCATCGAG GAGATTTCCA GAGCCATCAT GTGGGTCAAC 
GACAGAGAGG AGGAGGAGCT GGTGTTTGAC TGGGGCGATA AAAACATTGA CAACTACATC 
CCTCAAAAAC AAGAGAGCTA CTCGAAACTG ATGAGTGATC TGGAGGAGAA GGAGAAAGAG 
CTGAATAAGC TAAAGGCAAA GGTGGACATG CTGCTGAAGA ACCAGCACCC GGCATCTGAT 
AAGATAGAGG CTTACATGGA CACACTGCAA ACCCAGTGGA GCTGGTTACT GCAGATCACC 
AAGTGCATCC ATGTTCACCT GAAGGAGAAC GCAGCCTACA GCCAGTTCTT CAAGGAGGCC 
AATGAGACGT ACAGCAGACT GCAGAAAGAC CATGAAAACA TCCGGAAGAA GTTCGGCTGC 
GACAAAACCA CACCTCTGGA GAACCTCAAC GACATGCTGA AAAACCTGGA GAAAGAGAAG 
GAGAAGGTCA TCGAGAATAA GCGTCAGGTT CAGACGCTGG TCAGCAAGTC CAAGAACATC 
GTGTGTCTGA AACCTCGTAA TCCAGAGGAG AAGAGCAGCA GTCCGGTCAT AGTGCAAGCT 
CTGTGTGACT TCAAACAGGA TCAAAAAGGC ATTATGAAGG GCAATGAAGG TATCCTGAAA 
GACAACTCTC AGCGCAGTAA GTGGCACGTG ACTGGACCTG GAGGACTGGA AATGCTCATC 
CCGTCTGTGT GTCTGATCAT CCCACCGCCA AACCCTCTGA GCATCAGCCT GGCAAACAAG 
AACGAGCAGT ATTATGAAGC CATTATGTCC ATATGGAGTC AGCTGTACAT CAACATCAAG 
AGTCTGATAT CCTGGCAGTA CTGTCTGAGA GACATCCAGC ACATCAACTC CCTCACTCTC 
TCTATGCTGA CGCAAATGCG TCCTGAGGAG TATCGCCAAA CCATCAAGAA CCTGGAGATC 
CACTACCAGG AGTTTCTGCG CAACAGTCTT GGCTCTGAGA TGTTCGGTGA TGACGACAAG 
AGAAAGATGG AGCTTCAGTA TGCCGGAGCT CAGTCCCACT ACGACCAGCT GGTGATCCAA 
CTGCCCAACT ACAGGGAGAA TGGAGTGATC AGAGAGGTGG TGATGGTGGA GACAGATTCC 
AAGCTGAAAT CTGAGGTGTC GAGTGGGAAG ACTGCGACCG GGGTGTCGAG TGGGAAGACT 
GCGACAGGAG TGTCGAGTGG GAAGACTTCG TCTTCTGTGT CGGTTTCGGG TCTTAATGTG 
AGTCTGCTGT CGGACCTCAG CGCTCTGAGA CGGAGACTAG AGACAGCAGA ATCCGGGCTC 
ACCAAACATC TGCACGTTCC GCTGAAGGAG AACAGCGTTC AGGAGTGCTC ACAGAGACTG 
GCGCAGCTAC AGGCTGTTCA TCGTGATCTG GACTCCATCA GGGACGAGTA TCTGCATCTG 
AGGGAGAAGA TTATGAGAGA GCTGGAGGGC AGCAGTGACC CTGAGCAGAG CCGATACCTG 
CGCGCAGAAC TGGACCTGAT CAACCAGAAA CTGGGCAGCC TGCAGGGCTT CTCCAGCGCA 
TACATCCAGA GGCTCGGTGC TCTACAAGCT CTGCTCCAGC ACCTCCTGCA GGCCGAGGAC 
ATCATTAAAG TCCACGAGGC TCGTCTGACC GAGAAAGAAA CCTCTTCTCT TGACCTGAAC 
GAAGTGGAAA AGTACTGCAT GACTCTCAAG ACTATGAAAG CAGAGCTGGA GCAGAAGAAG 
GGTGTTTTGA AGGCGATGGA GACGGAGCTG TCTAAAGCTG TGCACTGGAA CAGTCAGATT 
GACCAGTCTT TCCATCAGTG TGATGTTGAC CTGTCCAGAT ACACTGAGCT GGTCGGACAG 
ATGACTGACC GCTGGCGCAG GATTGTGACT CAGATTGATA GCAGGACATG GGACCTGGAG 
AAGCAAGAGA AACAGCTGAA TCACTACCAG CAGACCAGCT CCACGATAAA CAGATGGATT 
CAGGACACAC GTCAGCGCCA AGACACCCTG CAGATTACCA AATTCAACAG CGTGGACAAT 
TTAATGGACC ACCTCAACCA GCAAAAAGCG CTTTATTCTG AGATCAAGGG GAAGAAAGAA 
AAGGTGGATG CTGTGGTCAA AGACTCAGAC ACATGTGCTG CCTCCATAAA GGACTACGAG 
CTCCAGTTGG CGTCTTATAG TGCAGGTCTG GAGACTCTGC TCAACATTCC CATCAAAAAG 
ACTATGCTGC AGTCTCCAGC CACTGTTCTC CGGGAGGAGG CAACAGATTT GCAGTCTCGC 
TACATTGAGC TCCTGACACG ATCAAGTGAC TACTACAAAT TCCTTGGGGA AATGCTGAAA 
AACATGGAGG AGTTAAAGAT GCGGAACACC AAAATTGAGC TTTTGGAGGA GGAACTGAGA 
CGCCTCAAGG ACAACCTCAA AGATCAAAAC CAGAAGAACA AATCACTGGA GGACAGTTTG 
ACTCGCTTCC GCCTGGAGCT GACCCAGTCC AAAGAGCAGC TCATATCTAT GGAGGAGGTG 
AAGAGAACCC AGGCCAGGCA GTGCAACACT GCTCAGGAAA GCCTGGATAG TACTCAGAAC 
CAGCTGAAGA GTTTACAAGA TGAGATGTCC CGCCTTACAT TCCTCATCGA GGAGGAAAAG 
CGCAAGCGAA GGCTGGCCGA GGAGCGTTAC ACCAACCAGC AGGAGGAGTA TGAGCTGGCC 
ATGCGCAAGA GACAGAAAGA ACTGGAGGAG CTCACTTTGT CCAAAAGTCA ATTTGAAAGA 
GCCATCAAAG AGAAAGAGCG GGAGATTGAA CGGCTGAAAT TACAGCTGCA GGATGAGGCC 
TCCCGACGCT CGGCAGCAGA ACTGGAGACC TCAAAGGTAA GAACCCAGTT GAACCAGGAC 
ATAAGTAGCC TAAAGCAAAC ATATGAATCT GAGATCCACG TCACCAAGAC CACAGTGCTC 
AAGTCGGCAC AGCAGAAGGA TGAGGAATCG GCATCTCTGA GACTTCAGGT GGACAGGTTA 
ACCTCTGAGA AGAGAGATCT GGAAGAGGAG CTAAGGAAAC TGCAGCTTTC TTTCTCCCAG 
ACGGAAGCGG CACGACGGAA AGCAGAAGAG GACGCGCATC AGAAGAGATC TATGGCTGCA 
GATGAAAGCA GATCCAAGAA GGAACTGGAG TCACAGATTC AGATCATAAT TCGTCAAAGG 
GAAGACATTG AAACTAGACA CAAGATGGAC CTGGCTGATG CAAACAGAGT TGCTCAGGAA 
AAGACCCGTG AGATCACATT GCTGACGCAA AATTTGCAAG ACGAGACCAG GCGGAGGAAA 
GCAATGGAGG TTGAGAATCA AAGCCTCAGA CAGTCTCAGG CTGAAATACT AGCCAAGCAG 
ACTTCAACCA CAGAGGTCAT CAACAAATTA AAGGTCTCTG AGCAAGAGGT TCTCATTATA 
AAGAGAGAGT TGGATGTACA GAAAAGTGAT AAGATGAAGT TGGAGCAGAA TGCCACCAGG 
TTGCAGAGTC GCATAAATGA ACTGCAGTCC AAGGTGAACG AGTTGCAGGC TGAACTGGAG 
AAAGAGAGAA GGAATAATCA GGATGAGCTC ACGAGGAGGA AACGCATGGA AACTGAGTTG 
GATAGGGTAA ATCAGACGTG CCGTGAATAC ACCACTACCA TCAGCACTCT GCGGGTCCAA 
CAAGAGAAAG AAAGTTCTTC TGGACGGAGG TACGAGCAGG AGCTTCGCAG TGCAAAAGAC 
GAGTTGGAGA GAAGCCTGAA AGAGTATAAA ATCACAGTAG AGAATCTGAC CAAAGTCAAT 
GCAGAACTGA AGGCTTTGCA ACAGCAGCTT GTACGGGAGC AGGGCCTGGT CCGAGAGGCT 
AATCAGCGAA ATGATTCCCT CTACAAGACT ATTGAGGAGA AGAGTCGACT ACTAAATGAG 
AGCACTAGCG AGGTTGAAAA GTTGCAGACC CTCACCCAGA ATCTTACCAA AGAGCGCCTG 
CGACTTGAGG AGGAACTAAG GAATGTGCGG CTCGAGCGGG ATGATGCTAA AAGAAGTCTG 
GGCACCATTG AGAGTGAAAG TGCTTCACGA TTGTCTGCCA TTCAGTTTCA GTTACAAACC 
AGCAATAACA GAGCAGTGGA ACTGCAGGAG CTAATCAATG AACTGACAAA AGAGAGGGAA 
AGTTTAAGGG TGGAAATCGC CCAAATCCAA AAGCAGTTCT CAGAGACATC CATGATGATC 
CAGCGATCTG ATTCAAACTA CAAAGACATT GTGCAAGAGA GAGATAGTCT CTTAATCAAA 
CTGAAGCTGC TGCAACAGGA CAAAGATAAG CAGCAGCGAT ATGAAGAGGA GCTCAGGCGC 
ATCAAACTTA CCTTGGAATC AGAGACCAAA CAGAAGCAAC GTCTCCAGGA TGAAATCGAT 
AAAATCACCA AGGATTTCAA GTACTGGAAA AGCCAGTACG AGTTAAAAGA GGGGCAGATC 
AGACAGAGCG AGATGGATAG AGACAGAGTA GAGAGAGACC GAGCCTCCTT ACAGAGTGAG 
ATCCAAAGAT TGACCGCTGA GCTGAGAAGT GTTGAGGAAC GCTACAGAGG TCGTCTTCAG 
AGCTCAGACA AGGAAATCTC GGAGCTAATG CGTAAGAAGG AGTCTTTGGA AATTGAGCTG 
AGGAGATTGC AACAGCGTCC AGCTGCAACC TGGAAGCAAA CCCAGACAGA TGAGATCTCT 
AAGCCAGTTG TTGAGCAGAA ATTGACAGTG CAAGGTCTTC GTGGTGAGGT CTCACTTACA 
GAGCTTGTCG AGTCCGACTT GCTCGATCAA ACTGATTTGG ACAAAATAAA CCGAGGACAG 
CTAACCAGTA AAGATATCGA GCACAAGCTC AAATCATACC TTGGTGGCTC TGATTGTATC 
GCTGGCATCT ATGATGAGGC CAAGGATAGA GTCATGCCTT TTTACCAGGC GATGAAAGAT 
GGTCTACTCC GACGAGGAAC CACACTGGAG CTCCTGGAGG CCCAGGCTGC CTCTGGTTTC 
ATAATTGACC CAGTTAACAA TGTCTGTATG ACCGTTGAAG AAGCTTGGAA GAGAGGTCTT 
GTAGGAAAGG AGTTCAAAGA CAAACTCCTG TCTGCTGAGA AGGCTGTGAC GGGATACAAA 
GATCCTGCAA CAGGAAAGAT CATCTCACTC TTCCAAGCTA TTGAGAAAGA GATCATTGAG 
AAAGGCCATG GAATCAGACT GCTGGAAGCT CAGATAGCCA GCGGTGGGAT TATTGATCCC 
AAAGGTAGCC ATCGAATTGA TGTGGAAGTA GCTTACAGGA AGGGTTACTT TGACCGTGAG 
ATGAATGAGA TTTTGTCCTA TGAAGGGGAT GACACCAAAG GTTTCTTTGA TCCCAACACC 
CATGAAAACC TCACATATCT AGAGTTGAAA AAGAGGTGCA TTAAAGATCC CAAGACTGGG 
CTCATGCTTT TGCCTTTAAA TGACAAGCAG AAACCAAAGC AGACGACAAC CCAGAAGAAC 
ACACTACGCA AAAGAAGAGT AGTGATCGTA GACCCTGACA CTGGTAAAGA GATGACTGTA 
CGAGAAGCGT ACCATCGAGA GTTAATCGAC TATGACACCT TTCTGGAACT TTCCGAACAA 
GAGTGCGAGT GGGAGGAGAT CACGATTGAA ACATCTGATG GAAACAAGCG TTTACTTATT 
GTCGATCGCA AAACTGGAAT CCAGTATGAC ATTCAGGAGT CCCTACAGAG GGGAATTATC 
AATAAACAAA CTTTGGAAAA GTACCGTGCA GGGACTATGA CGCTCACCGA GTTTGCAGCT 
CTGATCACTA GTAAAAGCAA CAGCTCTGAG CTTGCCATCT TCTCCAGCAG TCCTGAGGAT 
GTAGCCACCT GCAGCAGTCC CACACAGCCA TCATCCCCAA CCGTCCGCAA ACGATTTGCA 
AGTGTTTCCA TAACTCTATC CCCTCCATCT GATATTTTTG ACGACCAGAG CCCTGTGGGA 
GCAATATTCG ATACGGAGAC CCTTGAGAAG ATAACCATCC CCGAGGCTCA GCGACGTGGA 
ATAGTGGACA ACATCACCGC TCAGCGTCTT CTTGAAGCGC AGGTTTGCTC CGGAGGAATT 
ATCAATCCCG CTACAGGCCA GAGACTATCT CTGAAAGACG CCGTACAGCA AAGCCTCATC 
GATGAAGACA TGAGCGTCAA GCTAAAACCA GCGCAGAAGG CTTATGACGG TTTTGAGGAT 
GTGAAGACCA AGAGGAAACT TTCTGCTGCC GAAGCCATGA AGGAAAAATG GTTGCCCTAC 
GAAGCCGGCC AGAGGTTTCT AGAGTTCCAG TATCTCACAG GTGGACTCAT AGAGCCAGGA 
ACTGGACGCC GTGTCAGCAT TGAGGAGGCC ATCCGGAAAG GGTGGCTTGA TGGCAAAGGT 
GCACAAAAGC TGCAAGATAC CAGGAACTAC ATTAAGAACC TTACCTGTCC CAAAACCAAG 
CTTAAAATCT CATACAAAGA GGCGATGGAT AACTGCATGG TGGAGGAGAA CAATGGCATG 
AAGATGCTCC AGGCGACCTC CATGTCTACC AAAGGCATAA GCAGCCCCTA TAATGTGTCC 
TCTGGGCCAA GCTCTCGCTC TGGATCCAGA GCCGGATCCC GAACAGGCTC AAGAAGTGGA 
TCGCGCAGAG GCAGTGTCGA CTACAGCTCT TCATCTGTTT CTTACACCTT CTTCTCCTCT 
GCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919901.3
CDS	136..7644
Translation	MSLSGSQTRLHQISRRSSSRPDLTAVGYSLPRNDGAQFFTSGNGYQSDYNDGYMQTYTQTFSKNSQGGGAGGAQSMSSMAVQKRAQMLYSDCMGFLQRAQGLLETMGPASEVDKNMMAAQDAMDQLRKCAVDMRNAGLPNDMILRSLEDCHSFYADIRNSITGTTIRRTGGTVSGTIGGTISGTIGGTVGGWDDPSKSFQDALSWINQKKRLIETSGFGENSEAISQQILNHNKFHSSIQRSQEVDRARDDLMQSREKAGLHALDQEWDSLQKMSHARMDHLRDLQSIIEEISRAIMWVNDREEEELVFDWGDKNIDNYIPQKQESYSKLMSDLEEKEKELNKLKAKVDMLLKNQHPASDKIEAYMDTLQTQWSWLLQITKCIHVHLKENAAYSQFFKEANETYSRLQKDHENIRKKFGCDKTTPLENLNDMLKNLEKEKEKVIENKRQVQTLVSKSKNIVCLKPRNPEEKSSSPVIVQALCDFKQDQKGIMKGNEGILKDNSQRSKWHVTGPGGLEMLIPSVCLIIPPPNPLSISLANKNEQYYEAIMSIWSQLYINIKSLISWQYCLRDIQHINSLTLSMLTQMRPEEYRQTIKNLEIHYQEFLRNSLGSEMFGDDDKRKMELQYAGAQSHYDQLVIQLPNYRENGVIREVVMVETDSKLKSEVSSGKTATGVSSGKTATGVSSGKTSSSVSVSGLNVSLLSDLSALRRRLETAESGLTKHLHVPLKENSVQECSQRLAQLQAVHRDLDSIRDEYLHLREKIMRELEGSSDPEQSRYLRAELDLINQKLGSLQGFSSAYIQRLGALQALLQHLLQAEDIIKVHEARLTEKETSSLDLNEVEKYCMTLKTMKAELEQKKGVLKAMETELSKAVHWNSQIDQSFHQCDVDLSRYTELVGQMTDRWRRIVTQIDSRTWDLEKQEKQLNHYQQTSSTINRWIQDTRQRQDTLQITKFNSVDNLMDHLNQQKALYSEIKGKKEKVDAVVKDSDTCAASIKDYELQLASYSAGLETLLNIPIKKTMLQSPATVLREEATDLQSRYIELLTRSSDYYKFLGEMLKNMEELKMRNTKIELLEEELRRLKDNLKDQNQKNKSLEDSLTRFRLELTQSKEQLISMEEVKRTQARQCNTAQESLDSTQNQLKSLQDEMSRLTFLIEEEKRKRRLAEERYTNQQEEYELAMRKRQKELEELTLSKSQFERAIKEKEREIERLKLQLQDEASRRSAAELETSKVRTQLNQDISSLKQTYESEIHVTKTTVLKSAQQKDEESASLRLQVDRLTSEKRDLEEELRKLQLSFSQTEAARRKAEEDAHQKRSMAADESRSKKELESQIQIIIRQREDIETRHKMDLADANRVAQEKTREITLLTQNLQDETRRRKAMEVENQSLRQSQAEILAKQTSTTEVINKLKVSEQEVLIIKRELDVQKSDKMKLEQNATRLQSRINELQSKVNELQAELEKERRNNQDELTRRKRMETELDRVNQTCREYTTTISTLRVQQEKESSSGRRYEQELRSAKDELERSLKEYKITVENLTKVNAELKALQQQLVREQGLVREANQRNDSLYKTIEEKSRLLNESTSEVEKLQTLTQNLTKERLRLEEELRNVRLERDDAKRSLGTIESESASRLSAIQFQLQTSNNRAVELQELINELTKERESLRVEIAQIQKQFSETSMMIQRSDSNYKDIVQERDSLLIKLKLLQQDKDKQQRYEEELRRIKLTLESETKQKQRLQDEIDKITKDFKYWKSQYELKEGQIRQSEMDRDRVERDRASLQSEIQRLTAELRSVEERYRGRLQSSDKEISELMRKKESLEIELRRLQQRPAATWKQTQTDEISKPVVEQKLTVQGLRGEVSLTELVESDLLDQTDLDKINRGQLTSKDIEHKLKSYLGGSDCIAGIYDEAKDRVMPFYQAMKDGLLRRGTTLELLEAQAASGFIIDPVNNVCMTVEEAWKRGLVGKEFKDKLLSAEKAVTGYKDPATGKIISLFQAIEKEIIEKGHGIRLLEAQIASGGIIDPKGSHRIDVEVAYRKGYFDREMNEILSYEGDDTKGFFDPNTHENLTYLELKKRCIKDPKTGLMLLPLNDKQKPKQTTTQKNTLRKRRVVIVDPDTGKEMTVREAYHRELIDYDTFLELSEQECEWEEITIETSDGNKRLLIVDRKTGIQYDIQESLQRGIINKQTLEKYRAGTMTLTEFAALITSKSNSSELAIFSSSPEDVATCSSPTQPSSPTVRKRFASVSITLSPPSDIFDDQSPVGAIFDTETLEKITIPEAQRRGIVDNITAQRLLEAQVCSGGIINPATGQRLSLKDAVQQSLIDEDMSVKLKPAQKAYDGFEDVKTKRKLSAAEAMKEKWLPYEAGQRFLEFQYLTGGLIEPGTGRRVSIEEAIRKGWLDGKGAQKLQDTRNYIKNLTCPKTKLKISYKEAMDNCMVEENNGMKMLQATSMSTKGISSPYNVSSGPSSRSGSRAGSRTGSRSGSRRGSVDYSSSSVSYTFFSSAS

Target ORF information:

RefSeq Version	XM_001919866.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio desmoplakin a (dspa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919866.7

ORF Insert Sequence:

ATGAGTTTGT CCGGTTCTCA GACCAGGCTC CACCAGATCA GCCGCAGGTC CAGTTCGCGG 
CCAGACCTCA CCGCCGTCGG CTACAGTTTA CCGAGGAACG ACGGAGCTCA GTTTTTCACG 
AGTGGAAACG GATACCAGTC CGACTATAAT GACGGCTACA TGCAGACCTA CACGCAGACT 
TTCTCCAAAA ACTCTCAGGG TGGAGGAGCT GGAGGAGCCC AGAGCATGTC TTCCATGGCG 
GTTCAGAAGC GAGCTCAGAT GTTGTACAGT GACTGCATGG GATTCCTGCA GCGAGCGCAG 
GGCCTCCTGG AGACTATGGG ACCTGCTTCT GAGGTGGACA AGAACATGAT GGCAGCGCAG 
GACGCCATGG ATCAGCTGCG GAAATGTGCG GTGGACATGA GGAACGCCGG TCTGCCTAAT 
GACATGATCC TCCGCAGTTT GGAGGACTGT CATAGCTTTT ATGCAGACAT TCGCAACTCC 
ATCACGGGCA CCACCATCAG GAGGACTGGC GGCACCGTCA GCGGCACCAT CGGTGGCACC 
ATCAGTGGCA CCATTGGTGG CACTGTCGGC GGCTGGGACG ATCCCAGCAA GAGCTTTCAG 
GACGCACTGT CATGGATCAA CCAGAAGAAG AGACTCATTG AAACGTCAGG CTTCGGTGAA 
AATTCAGAAG CCATTAGCCA ACAAATCCTG AATCACAACA AGTTCCACAG TTCAATCCAG 
AGAAGCCAGG AGGTGGACAG AGCCCGCGAC GATCTGATGC AGAGCAGGGA AAAGGCTGGA 
CTACATGCTC TGGACCAAGA GTGGGACAGT TTACAGAAAA TGTCCCATGC ACGTATGGAT 
CATCTGCGAG ACCTACAGAG CATCATCGAG GAGATTTCCA GAGCCATCAT GTGGGTCAAC 
GACAGAGAGG AGGAGGAGCT GGTGTTTGAC TGGGGCGATA AAAACATTGA CAACTACATC 
CCTCAAAAAC AAGAGAGCTA CTCGAAACTG ATGAGTGATC TGGAGGAGAA GGAGAAAGAG 
CTGAATAAGC TAAAGGCAAA GGTGGACATG CTGCTGAAGA ACCAGCACCC GGCATCTGAT 
AAGATAGAGG CTTACATGGA CACACTGCAA ACCCAGTGGA GCTGGTTACT GCAGATCACC 
AAGTGCATCC ATGTTCACCT GAAGGAGAAC GCAGCCTACA GCCAGTTCTT CAAGGAGGCC 
AATGAGACGT ACAGCAGACT GCAGAAAGAC CATGAAAACA TCCGGAAGAA GTTCGGCTGC 
GACAAAACCA CACCTCTGGA GAACCTCAAC GACATGCTGA AAAACCTGGA GAAAGAGAAG 
GAGAAGGTCA TCGAGAATAA GCGTCAGGTT CAGACGCTGG TCAGCAAGTC CAAGAACATC 
GTGTGTCTGA AACCTCGTAA TCCAGAGGAG AAGAGCAGCA GTCCGGTCAT AGTGCAAGCT 
CTGTGTGACT TCAAACAGGA TCAAAAAGGC ATTATGAAGG GCAATGAAGG TATCCTGAAA 
GACAACTCTC AGCGCAGTAA GTGGCACGTG ACTGGACCTG GAGGACTGGA AATGCTCATC 
CCGTCTGTGT GTCTGATCAT CCCACCGCCA AACCCTCTGA GCATCAGCCT GGCAAACAAG 
AACGAGCAGT ATTATGAAGC CATTATGTCC ATATGGAGTC AGCTGTACAT CAACATCAAG 
AGTCTGATAT CCTGGCAGTA CTGTCTGAGA GACATCCAGC ACATCAACTC CCTCACTCTC 
TCTATGCTGA CGCAAATGCG TCCTGAGGAG TATCGCCAAA CCATCAAGAA CCTGGAGATC 
CACTACCAGG AGTTTCTGCG CAACAGTCTT GGCTCTGAGA TGTTCGGTGA TGACGACAAG 
AGAAAGATGG AGCTTCAGTA TGCCGGAGCT CAGTCCCACT ACGACCAGCT GGTGATCCAA 
CTGCCCAACT ACAGGGAGAA TGGAGTGATC AGAGAGGTGG TGATGGTGGA GACAGATTCC 
AAGCTGAAAT CTGAGGTGTC GAGTGGGAAG ACTGCGACCG GGGTGTCGAG TGGGAAGACT 
GCGACAGGAG TGTCGAGTGG GAAGACTTCG TCTTCTGTGT CGGTTTCGGG TCTTAATGTG 
AGTCTGCTGT CGGACCTCAG CGCTCTGAGA CGGAGACTAG AGACAGCAGA ATCCGGGCTC 
ACCAAACATC TGCACGTTCC GCTGAAGGAG AACAGCGTTC AGGAGTGCTC ACAGAGACTG 
GCGCAGCTAC AGGCTGTTCA TCGTGATCTG GACTCCATCA GGGACGAGTA TCTGCATCTG 
AGGGAGAAGA TTATGAGAGA GCTGGAGGGC AGCAGTGACC CTGAGCAGAG CCGATACCTG 
CGCGCAGAAC TGGACCTGAT CAACCAGAAA CTGGGCAGCC TGCAGGGCTT CTCCAGCGCA 
TACATCCAGA GGCTCGGTGC TCTACAAGCT CTGCTCCAGC ACCTCCTGCA GGCCGAGGAC 
ATCATTAAAG TCCACGAGGC TCGTCTGACC GAGAAAGAAA CCTCTTCTCT TGACCTGAAC 
GAAGTGGAAA AGTACTGCAT GACTCTCAAG ACTATGAAAG CAGAGCTGGA GCAGAAGAAG 
GGTGTTTTGA AGGCGATGGA GACGGAGCTG TCTAAAGCTG TGCACTGGAA CAGTCAGATT 
GACCAGTCTT TCCATCAGTG TGATGTTGAC CTGTCCAGAT ACACTGAGCT GGTCGGACAG 
ATGACTGACC GCTGGCGCAG GATTGTGACT CAGATTGATA GCAGGACATG GGACCTGGAG 
AAGCAAGAGA AACAGCTGAA TCACTACCAG CAGACCAGCT CCACGATAAA CAGATGGATT 
CAGGACACAC GTCAGCGCCA AGACACCCTG CAGATTACCA AATTCAACAG CGTGGACAAT 
TTAATGGACC ACCTCAACCA GCAAAAAGCG CTTTATTCTG AGATCAAGGG GAAGAAAGAA 
AAGGTGGATG CTGTGGTCAA AGACTCAGAC ACATGTGCTG CCTCCATAAA GGACTACGAG 
CTCCAGTTGG CGTCTTATAG TGCAGGTCTG GAGACTCTGC TCAACATTCC CATCAAAAAG 
ACTATGCTGC AGTCTCCAGC CACTGTTCTC CGGGAGGAGG CAACAGATTT GCAGTCTCGC 
TACATTGAGC TCCTGACACG ATCAAGTGAC TACTACAAAT TCCTTGGGGA AATGCTGAAA 
AACATGGAGG AGTTAAAGAT GCGGAACACC AAAATTGAGC TTTTGGAGGA GGAACTGAGA 
CGCCTCAAGG ACAACCTCAA AGATCAAAAC CAGAAGAACA AATCACTGGA GGACAGTTTG 
ACTCGCTTCC GCCTGGAGCT GACCCAGTCC AAAGAGCAGC TCATATCTAT GGAGGAGGTG 
AAGAGAACCC AGGCCAGGCA GTGCAACACT GCTCAGGAAA GCCTGGATAG TACTCAGAAC 
CAGCTGAAGA GTTTACAAGA TGAGATGTCC CGCCTTACAT TCCTCATCGA GGAGGAAAAG 
CGCAAGCGAA GGCTGGCCGA GGAGCGTTAC ACCAACCAGC AGGAGGAGTA TGAGCTGGCC 
ATGCGCAAGA GACAGAAAGA ACTGGAGGAG CTCACTTTGT CCAAAAGTCA ATTTGAAAGA 
GCCATCAAAG AGAAAGAGCG GGAGATTGAA CGGCTGAAAT TACAGCTGCA GGATGAGGCC 
TCCCGACGCT CGGCAGCAGA ACTGGAGACC TCAAAGGTAA GAACCCAGTT GAACCAGGAC 
ATAAGTAGCC TAAAGCAAAC ATATGAATCT GAGATCCACG TCACCAAGAC CACAGTGCTC 
AAGTCGGCAC AGCAGAAGGA TGAGGAATCG GCATCTCTGA GACTTCAGGT GGACAGGTTA 
ACCTCTGAGA AGAGAGATCT GGAAGAGGAG CTAAGGAAAC TGCAGCTTTC TTTCTCCCAG 
ACGGAAGCGG CACGACGGAA AGCAGAAGAG GACGCGCATC AGAAGAGATC TATGGCTGCA 
GATGAAAGCA GATCCAAGAA GGAACTGGAG TCACAGATTC AGATCATAAT TCGTCAAAGG 
GAAGACATTG AAACTAGACA CAAGATGGAC CTGGCTGATG CAAACAGAGT TGCTCAGGAA 
AAGACCCGTG AGATCACATT GCTGACGCAA AATTTGCAAG ACGAGACCAG GCGGAGGAAA 
GCAATGGAGG TTGAGAATCA AAGCCTCAGA CAGTCTCAGG CTGAAATACT AGCCAAGCAG 
ACTTCAACCA CAGAGGTCAT CAACAAATTA AAGGTCTCTG AGCAAGAGGT TCTCATTATA 
AAGAGAGAGT TGGATGTACA GAAAAGTGAT AAGATGAAGT TGGAGCAGAA TGCCACCAGG 
TTGCAGAGTC GCATAAATGA ACTGCAGTCC AAGGTGAACG AGTTGCAGGC TGAACTGGAG 
AAAGAGAGAA GGAATAATCA GGATGAGCTC ACGAGGAGGA AACGCATGGA AACTGAGTTG 
GATAGGGTAA ATCAGACGTG CCGTGAATAC ACCACTACCA TCAGCACTCT GCGGGTCCAA 
CAAGAGAAAG AAAGTTCTTC TGGACGGAGG TACGAGCAGG AGCTTCGCAG TGCAAAAGAC 
GAGTTGGAGA GAAGCCTGAA AGAGTATAAA ATCACAGTAG AGAATCTGAC CAAAGTCAAT 
GCAGAACTGA AGGCTTTGCA ACAGCAGCTT GTACGGGAGC AGGGCCTGGT CCGAGAGGCT 
AATCAGCGAA ATGATTCCCT CTACAAGACT ATTGAGGAGA AGAGTCGACT ACTAAATGAG 
AGCACTAGCG AGGTTGAAAA GTTGCAGACC CTCACCCAGA ATCTTACCAA AGAGCGCCTG 
CGACTTGAGG AGGAACTAAG GAATGTGCGG CTCGAGCGGG ATGATGCTAA AAGAAGTCTG 
GGCACCATTG AGAGTGAAAG TGCTTCACGA TTGTCTGCCA TTCAGTTTCA GTTACAAACC 
AGCAATAACA GAGCAGTGGA ACTGCAGGAG CTAATCAATG AACTGACAAA AGAGAGGGAA 
AGTTTAAGGG TGGAAATCGC CCAAATCCAA AAGCAGTTCT CAGAGACATC CATGATGATC 
CAGCGATCTG ATTCAAACTA CAAAGACATT GTGCAAGAGA GAGATAGTCT CTTAATCAAA 
CTGAAGCTGC TGCAACAGGA CAAAGATAAG CAGCAGCGAT ATGAAGAGGA GCTCAGGCGC 
ATCAAACTTA CCTTGGAATC AGAGACCAAA CAGAAGCAAC GTCTCCAGGA TGAAATCGAT 
AAAATCACCA AGGATTTCAA GTACTGGAAA AGCCAGTACG AGTTAAAAGA GGGGCAGATC 
AGACAGAGCG AGATGGATAG AGACAGAGTA GAGAGAGACC GAGCCTCCTT ACAGAGTGAG 
ATCCAAAGAT TGACCGCTGA GCTGAGAAGT GTTGAGGAAC GCTACAGAGG TCGTCTTCAG 
AGCTCAGACA AGGAAATCTC GGAGCTAATG CGTAAGAAGG AGTCTTTGGA AATTGAGCTG 
AGGAGATTGC AACAGCGTCC AGCTGCAACC TGGAAGCAAA CCCAGACAGA TGAGATCTCT 
AAGCCAGTTG TTGAGCAGAA ATTGACAGTG CAAGGTCTTC GTGGTGAGGT CTCACTTACA 
GAGCTTGTCG AGTCCGACTT GCTCGATCAA ACTGATTTGG ACAAAATAAA CCGAGGACAG 
CTAACCAGTA AAGATATCGA GCACAAGCTC AAATCATACC TTGGTGGCTC TGATTGTATC 
GCTGGCATCT ATGATGAGGC CAAGGATAGA GTCATGCCTT TTTACCAGGC GATGAAAGAT 
GGTCTACTCC GACGAGGAAC CACACTGGAG CTCCTGGAGG CCCAGGCTGC CTCTGGTTTC 
ATAATTGACC CAGTTAACAA TGTCTGTATG ACCGTTGAAG AAGCTTGGAA GAGAGGTCTT 
GTAGGAAAGG AGTTCAAAGA CAAACTCCTG TCTGCTGAGA AGGCTGTGAC GGGATACAAA 
GATCCTGCAA CAGGAAAGAT CATCTCACTC TTCCAAGCTA TTGAGAAAGA GATCATTGAG 
AAAGGCCATG GAATCAGACT GCTGGAAGCT CAGATAGCCA GCGGTGGGAT TATTGATCCC 
AAAGGTAGCC ATCGAATTGA TGTGGAAGTA GCTTACAGGA AGGGTTACTT TGACCGTGAG 
ATGAATGAGA TTTTGTCCTA TGAAGGGGAT GACACCAAAG GTTTCTTTGA TCCCAACACC 
CATGAAAACC TCACATATCT AGAGTTGAAA AAGAGGTGCA TTAAAGATCC CAAGACTGGG 
CTCATGCTTT TGCCTTTAAA TGACAAGCAG AAACCAAAGC AGACGACAAC CCAGAAGAAC 
ACACTACGCA AAAGAAGAGT AGTGATCGTA GACCCTGACA CTGGTAAAGA GATGACTGTA 
CGAGAAGCGT ACCATCGAGA GTTAATCGAC TATGACACCT TTCTGGAACT TTCCGAACAA 
GAGTGCGAGT GGGAGGAGAT CACGATTGAA ACATCTGATG GAAACAAGCG TTTACTTATT 
GTCGATCGCA AAACTGGAAT CCAGTATGAC ATTCAGGAGT CCCTACAGAG GGGAATTATC 
AATAAACAAA CTTTGGAAAA GTACCGTGCA GGGACTATGA CGCTCACCGA GTTTGCAGCT 
CTGATCACTA GTAAAAGCAA CAGCTCTGAG CTTGCCATCT TCTCCAGCAG TCCTGAGGAT 
GTAGCCACCT GCAGCAGTCC CACACAGCCA TCATCCCCAA CCGTCCGCAA ACGATTTGCA 
AGTGTTTCCA TAACTCTATC CCCTCCATCT GATATTTTTG ACGACCAGAG CCCTGTGGGA 
GCAATATTCG ATACGGAGAC CCTTGAGAAG ATAACCATCC CCGAGGCTCA GCGACGTGGA 
ATAGTGGACA ACATCACCGC TCAGCGTCTT CTTGAAGCGC AGGTTTGCTC CGGAGGAATT 
ATCAATCCCG CTACAGGCCA GAGACTATCT CTGAAAGACG CCGTACAGCA AAGCCTCATC 
GATGAAGACA TGAGCGTCAA GCTAAAACCA GCGCAGAAGG CTTATGACGG TTTTGAGGAT 
GTGAAGACCA AGAGGAAACT TTCTGCTGCC GAAGCCATGA AGGAAAAATG GTTGCCCTAC 
GAAGCCGGCC AGAGGTTTCT AGAGTTCCAG TATCTCACAG GTGGACTCAT AGAGCCAGGA 
ACTGGACGCC GTGTCAGCAT TGAGGAGGCC ATCCGGAAAG GGTGGCTTGA TGGCAAAGGT 
GCACAAAAGC TGCAAGATAC CAGGAACTAC ATTAAGAACC TTACCTGTCC CAAAACCAAG 
CTTAAAATCT CATACAAAGA GGCGATGGAT AACTGCATGG TGGAGGAGAA CAATGGCATG 
AAGATGCTCC AGGCGACCTC CATGTCTACC AAAGGCATAA GCAGCCCCTA TAATGTGTCC 
TCTGGGCCAA GCTCTCGCTC TGGATCCAGA GCCGGATCCC GAACAGGCTC AAGAAGTGGA 
TCGCGCAGAG GCAGTGTCGA CTACAGCTCT TCATCTGTTT CTTACACCTT CTTCTCCTCT 
GCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dspa ( XM_001919866.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001919901.3 Danio rerio desmoplakin a (dspa), transcript variant X1, mRNA.

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CloneID	ODa06595
Clone ID Related Accession (Same CDS sequence)	XM_001919866.7 , XM_001919866.6
Accession Version	XM_001919866.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	desmoplakin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919866.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGT CCGGTTCTCA GACCAGGCTC CACCAGATCA GCCGCAGGTC CAGTTCGCGG 
CCAGACCTCA CCGCCGTCGG CTACAGTTTA CCGAGGAACG ACGGAGCTCA GTTTTTCACG 
AGTGGAAACG GATACCAGTC CGACTATAAT GACGGCTACA TGCAGACCTA CACGCAGACT 
TTCTCCAAAA ACTCTCAGGG TGGAGGAGCT GGAGGAGCCC AGAGCATGTC TTCCATGGCG 
GTTCAGAAGC GAGCTCAGAT GTTGTACAGT GACTGCATGG GATTCCTGCA GCGAGCGCAG 
GGCCTCCTGG AGACTATGGG ACCTGCTTCT GAGGTGGACA AGAACATGAT GGCAGCGCAG 
GACGCCATGG ATCAGCTGCG GAAATGTGCG GTGGACATGA GGAACGCCGG TCTGCCTAAT 
GACATGATCC TCCGCAGTTT GGAGGACTGT CATAGCTTTT ATGCAGACAT TCGCAACTCC 
ATCACGGGCA CCACCATCAG GAGGACTGGC GGCACCGTCA GCGGCACCAT CGGTGGCACC 
ATCAGTGGCA CCATTGGTGG CACTGTCGGC GGCTGGGACG ATCCCAGCAA GAGCTTTCAG 
GACGCACTGT CATGGATCAA CCAGAAGAAG AGACTCATTG AAACGTCAGG CTTCGGTGAA 
AATTCAGAAG CCATTAGCCA ACAAATCCTG AATCACAACA AGTTCCACAG TTCAATCCAG 
AGAAGCCAGG AGGTGGACAG AGCCCGCGAC GATCTGATGC AGAGCAGGGA AAAGGCTGGA 
CTACATGCTC TGGACCAAGA GTGGGACAGT TTACAGAAAA TGTCCCATGC ACGTATGGAT 
CATCTGCGAG ACCTACAGAG CATCATCGAG GAGATTTCCA GAGCCATCAT GTGGGTCAAC 
GACAGAGAGG AGGAGGAGCT GGTGTTTGAC TGGGGCGATA AAAACATTGA CAACTACATC 
CCTCAAAAAC AAGAGAGCTA CTCGAAACTG ATGAGTGATC TGGAGGAGAA GGAGAAAGAG 
CTGAATAAGC TAAAGGCAAA GGTGGACATG CTGCTGAAGA ACCAGCACCC GGCATCTGAT 
AAGATAGAGG CTTACATGGA CACACTGCAA ACCCAGTGGA GCTGGTTACT GCAGATCACC 
AAGTGCATCC ATGTTCACCT GAAGGAGAAC GCAGCCTACA GCCAGTTCTT CAAGGAGGCC 
AATGAGACGT ACAGCAGACT GCAGAAAGAC CATGAAAACA TCCGGAAGAA GTTCGGCTGC 
GACAAAACCA CACCTCTGGA GAACCTCAAC GACATGCTGA AAAACCTGGA GAAAGAGAAG 
GAGAAGGTCA TCGAGAATAA GCGTCAGGTT CAGACGCTGG TCAGCAAGTC CAAGAACATC 
GTGTGTCTGA AACCTCGTAA TCCAGAGGAG AAGAGCAGCA GTCCGGTCAT AGTGCAAGCT 
CTGTGTGACT TCAAACAGGA TCAAAAAGGC ATTATGAAGG GCAATGAAGG TATCCTGAAA 
GACAACTCTC AGCGCAGTAA GTGGCACGTG ACTGGACCTG GAGGACTGGA AATGCTCATC 
CCGTCTGTGT GTCTGATCAT CCCACCGCCA AACCCTCTGA GCATCAGCCT GGCAAACAAG 
AACGAGCAGT ATTATGAAGC CATTATGTCC ATATGGAGTC AGCTGTACAT CAACATCAAG 
AGTCTGATAT CCTGGCAGTA CTGTCTGAGA GACATCCAGC ACATCAACTC CCTCACTCTC 
TCTATGCTGA CGCAAATGCG TCCTGAGGAG TATCGCCAAA CCATCAAGAA CCTGGAGATC 
CACTACCAGG AGTTTCTGCG CAACAGTCTT GGCTCTGAGA TGTTCGGTGA TGACGACAAG 
AGAAAGATGG AGCTTCAGTA TGCCGGAGCT CAGTCCCACT ACGACCAGCT GGTGATCCAA 
CTGCCCAACT ACAGGGAGAA TGGAGTGATC AGAGAGGTGG TGATGGTGGA GACAGATTCC 
AAGCTGAAAT CTGAGGTGTC GAGTGGGAAG ACTGCGACCG GGGTGTCGAG TGGGAAGACT 
GCGACAGGAG TGTCGAGTGG GAAGACTTCG TCTTCTGTGT CGGTTTCGGG TCTTAATGTG 
AGTCTGCTGT CGGACCTCAG CGCTCTGAGA CGGAGACTAG AGACAGCAGA ATCCGGGCTC 
ACCAAACATC TGCACGTTCC GCTGAAGGAG AACAGCGTTC AGGAGTGCTC ACAGAGACTG 
GCGCAGCTAC AGGCTGTTCA TCGTGATCTG GACTCCATCA GGGACGAGTA TCTGCATCTG 
AGGGAGAAGA TTATGAGAGA GCTGGAGGGC AGCAGTGACC CTGAGCAGAG CCGATACCTG 
CGCGCAGAAC TGGACCTGAT CAACCAGAAA CTGGGCAGCC TGCAGGGCTT CTCCAGCGCA 
TACATCCAGA GGCTCGGTGC TCTACAAGCT CTGCTCCAGC ACCTCCTGCA GGCCGAGGAC 
ATCATTAAAG TCCACGAGGC TCGTCTGACC GAGAAAGAAA CCTCTTCTCT TGACCTGAAC 
GAAGTGGAAA AGTACTGCAT GACTCTCAAG ACTATGAAAG CAGAGCTGGA GCAGAAGAAG 
GGTGTTTTGA AGGCGATGGA GACGGAGCTG TCTAAAGCTG TGCACTGGAA CAGTCAGATT 
GACCAGTCTT TCCATCAGTG TGATGTTGAC CTGTCCAGAT ACACTGAGCT GGTCGGACAG 
ATGACTGACC GCTGGCGCAG GATTGTGACT CAGATTGATA GCAGGACATG GGACCTGGAG 
AAGCAAGAGA AACAGCTGAA TCACTACCAG CAGACCAGCT CCACGATAAA CAGATGGATT 
CAGGACACAC GTCAGCGCCA AGACACCCTG CAGATTACCA AATTCAACAG CGTGGACAAT 
TTAATGGACC ACCTCAACCA GCAAAAAGCG CTTTATTCTG AGATCAAGGG GAAGAAAGAA 
AAGGTGGATG CTGTGGTCAA AGACTCAGAC ACATGTGCTG CCTCCATAAA GGACTACGAG 
CTCCAGTTGG CGTCTTATAG TGCAGGTCTG GAGACTCTGC TCAACATTCC CATCAAAAAG 
ACTATGCTGC AGTCTCCAGC CACTGTTCTC CGGGAGGAGG CAACAGATTT GCAGTCTCGC 
TACATTGAGC TCCTGACACG ATCAAGTGAC TACTACAAAT TCCTTGGGGA AATGCTGAAA 
AACATGGAGG AGTTAAAGAT GCGGAACACC AAAATTGAGC TTTTGGAGGA GGAACTGAGA 
CGCCTCAAGG ACAACCTCAA AGATCAAAAC CAGAAGAACA AATCACTGGA GGACAGTTTG 
ACTCGCTTCC GCCTGGAGCT GACCCAGTCC AAAGAGCAGC TCATATCTAT GGAGGAGGTG 
AAGAGAACCC AGGCCAGGCA GTGCAACACT GCTCAGGAAA GCCTGGATAG TACTCAGAAC 
CAGCTGAAGA GTTTACAAGA TGAGATGTCC CGCCTTACAT TCCTCATCGA GGAGGAAAAG 
CGCAAGCGAA GGCTGGCCGA GGAGCGTTAC ACCAACCAGC AGGAGGAGTA TGAGCTGGCC 
ATGCGCAAGA GACAGAAAGA ACTGGAGGAG CTCACTTTGT CCAAAAGTCA ATTTGAAAGA 
GCCATCAAAG AGAAAGAGCG GGAGATTGAA CGGCTGAAAT TACAGCTGCA GGATGAGGCC 
TCCCGACGCT CGGCAGCAGA ACTGGAGACC TCAAAGGTAA GAACCCAGTT GAACCAGGAC 
ATAAGTAGCC TAAAGCAAAC ATATGAATCT GAGATCCACG TCACCAAGAC CACAGTGCTC 
AAGTCGGCAC AGCAGAAGGA TGAGGAATCG GCATCTCTGA GACTTCAGGT GGACAGGTTA 
ACCTCTGAGA AGAGAGATCT GGAAGAGGAG CTAAGGAAAC TGCAGCTTTC TTTCTCCCAG 
ACGGAAGCGG CACGACGGAA AGCAGAAGAG GACGCGCATC AGAAGAGATC TATGGCTGCA 
GATGAAAGCA GATCCAAGAA GGAACTGGAG TCACAGATTC AGATCATAAT TCGTCAAAGG 
GAAGACATTG AAACTAGACA CAAGATGGAC CTGGCTGATG CAAACAGAGT TGCTCAGGAA 
AAGACCCGTG AGATCACATT GCTGACGCAA AATTTGCAAG ACGAGACCAG GCGGAGGAAA 
GCAATGGAGG TTGAGAATCA AAGCCTCAGA CAGTCTCAGG CTGAAATACT AGCCAAGCAG 
ACTTCAACCA CAGAGGTCAT CAACAAATTA AAGGTCTCTG AGCAAGAGGT TCTCATTATA 
AAGAGAGAGT TGGATGTACA GAAAAGTGAT AAGATGAAGT TGGAGCAGAA TGCCACCAGG 
TTGCAGAGTC GCATAAATGA ACTGCAGTCC AAGGTGAACG AGTTGCAGGC TGAACTGGAG 
AAAGAGAGAA GGAATAATCA GGATGAGCTC ACGAGGAGGA AACGCATGGA AACTGAGTTG 
GATAGGGTAA ATCAGACGTG CCGTGAATAC ACCACTACCA TCAGCACTCT GCGGGTCCAA 
CAAGAGAAAG AAAGTTCTTC TGGACGGAGG TACGAGCAGG AGCTTCGCAG TGCAAAAGAC 
GAGTTGGAGA GAAGCCTGAA AGAGTATAAA ATCACAGTAG AGAATCTGAC CAAAGTCAAT 
GCAGAACTGA AGGCTTTGCA ACAGCAGCTT GTACGGGAGC AGGGCCTGGT CCGAGAGGCT 
AATCAGCGAA ATGATTCCCT CTACAAGACT ATTGAGGAGA AGAGTCGACT ACTAAATGAG 
AGCACTAGCG AGGTTGAAAA GTTGCAGACC CTCACCCAGA ATCTTACCAA AGAGCGCCTG 
CGACTTGAGG AGGAACTAAG GAATGTGCGG CTCGAGCGGG ATGATGCTAA AAGAAGTCTG 
GGCACCATTG AGAGTGAAAG TGCTTCACGA TTGTCTGCCA TTCAGTTTCA GTTACAAACC 
AGCAATAACA GAGCAGTGGA ACTGCAGGAG CTAATCAATG AACTGACAAA AGAGAGGGAA 
AGTTTAAGGG TGGAAATCGC CCAAATCCAA AAGCAGTTCT CAGAGACATC CATGATGATC 
CAGCGATCTG ATTCAAACTA CAAAGACATT GTGCAAGAGA GAGATAGTCT CTTAATCAAA 
CTGAAGCTGC TGCAACAGGA CAAAGATAAG CAGCAGCGAT ATGAAGAGGA GCTCAGGCGC 
ATCAAACTTA CCTTGGAATC AGAGACCAAA CAGAAGCAAC GTCTCCAGGA TGAAATCGAT 
AAAATCACCA AGGATTTCAA GTACTGGAAA AGCCAGTACG AGTTAAAAGA GGGGCAGATC 
AGACAGAGCG AGATGGATAG AGACAGAGTA GAGAGAGACC GAGCCTCCTT ACAGAGTGAG 
ATCCAAAGAT TGACCGCTGA GCTGAGAAGT GTTGAGGAAC GCTACAGAGG TCGTCTTCAG 
AGCTCAGACA AGGAAATCTC GGAGCTAATG CGTAAGAAGG AGTCTTTGGA AATTGAGCTG 
AGGAGATTGC AACAGCGTCC AGCTGCAACC TGGAAGCAAA CCCAGACAGA TGAGATCTCT 
AAGCCAGTTG TTGAGCAGAA ATTGACAGTG CAAGGTCTTC GTGGTGAGGT CTCACTTACA 
GAGCTTGTCG AGTCCGACTT GCTCGATCAA ACTGATTTGG ACAAAATAAA CCGAGGACAG 
CTAACCAGTA AAGATATCGA GCACAAGCTC AAATCATACC TTGGTGGCTC TGATTGTATC 
GCTGGCATCT ATGATGAGGC CAAGGATAGA GTCATGCCTT TTTACCAGGC GATGAAAGAT 
GGTCTACTCC GACGAGGAAC CACACTGGAG CTCCTGGAGG CCCAGGCTGC CTCTGGTTTC 
ATAATTGACC CAGTTAACAA TGTCTGTATG ACCGTTGAAG AAGCTTGGAA GAGAGGTCTT 
GTAGGAAAGG AGTTCAAAGA CAAACTCCTG TCTGCTGAGA AGGCTGTGAC GGGATACAAA 
GATCCTGCAA CAGGAAAGAT CATCTCACTC TTCCAAGCTA TTGAGAAAGA GATCATTGAG 
AAAGGCCATG GAATCAGACT GCTGGAAGCT CAGATAGCCA GCGGTGGGAT TATTGATCCC 
AAAGGTAGCC ATCGAATTGA TGTGGAAGTA GCTTACAGGA AGGGTTACTT TGACCGTGAG 
ATGAATGAGA TTTTGTCCTA TGAAGGGGAT GACACCAAAG GTTTCTTTGA TCCCAACACC 
CATGAAAACC TCACATATCT AGAGTTGAAA AAGAGGTGCA TTAAAGATCC CAAGACTGGG 
CTCATGCTTT TGCCTTTAAA TGACAAGCAG AAACCAAAGC AGACGACAAC CCAGAAGAAC 
ACACTACGCA AAAGAAGAGT AGTGATCGTA GACCCTGACA CTGGTAAAGA GATGACTGTA 
CGAGAAGCGT ACCATCGAGA GTTAATCGAC TATGACACCT TTCTGGAACT TTCCGAACAA 
GAGTGCGAGT GGGAGGAGAT CACGATTGAA ACATCTGATG GAAACAAGCG TTTACTTATT 
GTCGATCGCA AAACTGGAAT CCAGTATGAC ATTCAGGAGT CCCTACAGAG GGGAATTATC 
AATAAACAAA CTTTGGAAAA GTACCGTGCA GGGACTATGA CGCTCACCGA GTTTGCAGCT 
CTGATCACTA GTAAAAGCAA CAGCTCTGAG CTTGCCATCT TCTCCAGCAG TCCTGAGGAT 
GTAGCCACCT GCAGCAGTCC CACACAGCCA TCATCCCCAA CCGTCCGCAA ACGATTTGCA 
AGTGTTTCCA TAACTCTATC CCCTCCATCT GATATTTTTG ACGACCAGAG CCCTGTGGGA 
GCAATATTCG ATACGGAGAC CCTTGAGAAG ATAACCATCC CCGAGGCTCA GCGACGTGGA 
ATAGTGGACA ACATCACCGC TCAGCGTCTT CTTGAAGCGC AGGTTTGCTC CGGAGGAATT 
ATCAATCCCG CTACAGGCCA GAGACTATCT CTGAAAGACG CCGTACAGCA AAGCCTCATC 
GATGAAGACA TGAGCGTCAA GCTAAAACCA GCGCAGAAGG CTTATGACGG TTTTGAGGAT 
GTGAAGACCA AGAGGAAACT TTCTGCTGCC GAAGCCATGA AGGAAAAATG GTTGCCCTAC 
GAAGCCGGCC AGAGGTTTCT AGAGTTCCAG TATCTCACAG GTGGACTCAT AGAGCCAGGA 
ACTGGACGCC GTGTCAGCAT TGAGGAGGCC ATCCGGAAAG GGTGGCTTGA TGGCAAAGGT 
GCACAAAAGC TGCAAGATAC CAGGAACTAC ATTAAGAACC TTACCTGTCC CAAAACCAAG 
CTTAAAATCT CATACAAAGA GGCGATGGAT AACTGCATGG TGGAGGAGAA CAATGGCATG 
AAGATGCTCC AGGCGACCTC CATGTCTACC AAAGGCATAA GCAGCCCCTA TAATGTGTCC 
TCTGGGCCAA GCTCTCGCTC TGGATCCAGA GCCGGATCCC GAACAGGCTC AAGAAGTGGA 
TCGCGCAGAG GCAGTGTCGA CTACAGCTCT TCATCTGTTT CTTACACCTT CTTCTCCTCT 
GCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919901.3
CDS	133..7641
Translation	MSLSGSQTRLHQISRRSSSRPDLTAVGYSLPRNDGAQFFTSGNGYQSDYNDGYMQTYTQTFSKNSQGGGAGGAQSMSSMAVQKRAQMLYSDCMGFLQRAQGLLETMGPASEVDKNMMAAQDAMDQLRKCAVDMRNAGLPNDMILRSLEDCHSFYADIRNSITGTTIRRTGGTVSGTIGGTISGTIGGTVGGWDDPSKSFQDALSWINQKKRLIETSGFGENSEAISQQILNHNKFHSSIQRSQEVDRARDDLMQSREKAGLHALDQEWDSLQKMSHARMDHLRDLQSIIEEISRAIMWVNDREEEELVFDWGDKNIDNYIPQKQESYSKLMSDLEEKEKELNKLKAKVDMLLKNQHPASDKIEAYMDTLQTQWSWLLQITKCIHVHLKENAAYSQFFKEANETYSRLQKDHENIRKKFGCDKTTPLENLNDMLKNLEKEKEKVIENKRQVQTLVSKSKNIVCLKPRNPEEKSSSPVIVQALCDFKQDQKGIMKGNEGILKDNSQRSKWHVTGPGGLEMLIPSVCLIIPPPNPLSISLANKNEQYYEAIMSIWSQLYINIKSLISWQYCLRDIQHINSLTLSMLTQMRPEEYRQTIKNLEIHYQEFLRNSLGSEMFGDDDKRKMELQYAGAQSHYDQLVIQLPNYRENGVIREVVMVETDSKLKSEVSSGKTATGVSSGKTATGVSSGKTSSSVSVSGLNVSLLSDLSALRRRLETAESGLTKHLHVPLKENSVQECSQRLAQLQAVHRDLDSIRDEYLHLREKIMRELEGSSDPEQSRYLRAELDLINQKLGSLQGFSSAYIQRLGALQALLQHLLQAEDIIKVHEARLTEKETSSLDLNEVEKYCMTLKTMKAELEQKKGVLKAMETELSKAVHWNSQIDQSFHQCDVDLSRYTELVGQMTDRWRRIVTQIDSRTWDLEKQEKQLNHYQQTSSTINRWIQDTRQRQDTLQITKFNSVDNLMDHLNQQKALYSEIKGKKEKVDAVVKDSDTCAASIKDYELQLASYSAGLETLLNIPIKKTMLQSPATVLREEATDLQSRYIELLTRSSDYYKFLGEMLKNMEELKMRNTKIELLEEELRRLKDNLKDQNQKNKSLEDSLTRFRLELTQSKEQLISMEEVKRTQARQCNTAQESLDSTQNQLKSLQDEMSRLTFLIEEEKRKRRLAEERYTNQQEEYELAMRKRQKELEELTLSKSQFERAIKEKEREIERLKLQLQDEASRRSAAELETSKVRTQLNQDISSLKQTYESEIHVTKTTVLKSAQQKDEESASLRLQVDRLTSEKRDLEEELRKLQLSFSQTEAARRKAEEDAHQKRSMAADESRSKKELESQIQIIIRQREDIETRHKMDLADANRVAQEKTREITLLTQNLQDETRRRKAMEVENQSLRQSQAEILAKQTSTTEVINKLKVSEQEVLIIKRELDVQKSDKMKLEQNATRLQSRINELQSKVNELQAELEKERRNNQDELTRRKRMETELDRVNQTCREYTTTISTLRVQQEKESSSGRRYEQELRSAKDELERSLKEYKITVENLTKVNAELKALQQQLVREQGLVREANQRNDSLYKTIEEKSRLLNESTSEVEKLQTLTQNLTKERLRLEEELRNVRLERDDAKRSLGTIESESASRLSAIQFQLQTSNNRAVELQELINELTKERESLRVEIAQIQKQFSETSMMIQRSDSNYKDIVQERDSLLIKLKLLQQDKDKQQRYEEELRRIKLTLESETKQKQRLQDEIDKITKDFKYWKSQYELKEGQIRQSEMDRDRVERDRASLQSEIQRLTAELRSVEERYRGRLQSSDKEISELMRKKESLEIELRRLQQRPAATWKQTQTDEISKPVVEQKLTVQGLRGEVSLTELVESDLLDQTDLDKINRGQLTSKDIEHKLKSYLGGSDCIAGIYDEAKDRVMPFYQAMKDGLLRRGTTLELLEAQAASGFIIDPVNNVCMTVEEAWKRGLVGKEFKDKLLSAEKAVTGYKDPATGKIISLFQAIEKEIIEKGHGIRLLEAQIASGGIIDPKGSHRIDVEVAYRKGYFDREMNEILSYEGDDTKGFFDPNTHENLTYLELKKRCIKDPKTGLMLLPLNDKQKPKQTTTQKNTLRKRRVVIVDPDTGKEMTVREAYHRELIDYDTFLELSEQECEWEEITIETSDGNKRLLIVDRKTGIQYDIQESLQRGIINKQTLEKYRAGTMTLTEFAALITSKSNSSELAIFSSSPEDVATCSSPTQPSSPTVRKRFASVSITLSPPSDIFDDQSPVGAIFDTETLEKITIPEAQRRGIVDNITAQRLLEAQVCSGGIINPATGQRLSLKDAVQQSLIDEDMSVKLKPAQKAYDGFEDVKTKRKLSAAEAMKEKWLPYEAGQRFLEFQYLTGGLIEPGTGRRVSIEEAIRKGWLDGKGAQKLQDTRNYIKNLTCPKTKLKISYKEAMDNCMVEENNGMKMLQATSMSTKGISSPYNVSSGPSSRSGSRAGSRTGSRSGSRRGSVDYSSSSVSYTFFSSAS

Target ORF information:

RefSeq Version	XM_001919866.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio desmoplakin a (dspa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919866.6

ORF Insert Sequence:

ATGAGTTTGT CCGGTTCTCA GACCAGGCTC CACCAGATCA GCCGCAGGTC CAGTTCGCGG 
CCAGACCTCA CCGCCGTCGG CTACAGTTTA CCGAGGAACG ACGGAGCTCA GTTTTTCACG 
AGTGGAAACG GATACCAGTC CGACTATAAT GACGGCTACA TGCAGACCTA CACGCAGACT 
TTCTCCAAAA ACTCTCAGGG TGGAGGAGCT GGAGGAGCCC AGAGCATGTC TTCCATGGCG 
GTTCAGAAGC GAGCTCAGAT GTTGTACAGT GACTGCATGG GATTCCTGCA GCGAGCGCAG 
GGCCTCCTGG AGACTATGGG ACCTGCTTCT GAGGTGGACA AGAACATGAT GGCAGCGCAG 
GACGCCATGG ATCAGCTGCG GAAATGTGCG GTGGACATGA GGAACGCCGG TCTGCCTAAT 
GACATGATCC TCCGCAGTTT GGAGGACTGT CATAGCTTTT ATGCAGACAT TCGCAACTCC 
ATCACGGGCA CCACCATCAG GAGGACTGGC GGCACCGTCA GCGGCACCAT CGGTGGCACC 
ATCAGTGGCA CCATTGGTGG CACTGTCGGC GGCTGGGACG ATCCCAGCAA GAGCTTTCAG 
GACGCACTGT CATGGATCAA CCAGAAGAAG AGACTCATTG AAACGTCAGG CTTCGGTGAA 
AATTCAGAAG CCATTAGCCA ACAAATCCTG AATCACAACA AGTTCCACAG TTCAATCCAG 
AGAAGCCAGG AGGTGGACAG AGCCCGCGAC GATCTGATGC AGAGCAGGGA AAAGGCTGGA 
CTACATGCTC TGGACCAAGA GTGGGACAGT TTACAGAAAA TGTCCCATGC ACGTATGGAT 
CATCTGCGAG ACCTACAGAG CATCATCGAG GAGATTTCCA GAGCCATCAT GTGGGTCAAC 
GACAGAGAGG AGGAGGAGCT GGTGTTTGAC TGGGGCGATA AAAACATTGA CAACTACATC 
CCTCAAAAAC AAGAGAGCTA CTCGAAACTG ATGAGTGATC TGGAGGAGAA GGAGAAAGAG 
CTGAATAAGC TAAAGGCAAA GGTGGACATG CTGCTGAAGA ACCAGCACCC GGCATCTGAT 
AAGATAGAGG CTTACATGGA CACACTGCAA ACCCAGTGGA GCTGGTTACT GCAGATCACC 
AAGTGCATCC ATGTTCACCT GAAGGAGAAC GCAGCCTACA GCCAGTTCTT CAAGGAGGCC 
AATGAGACGT ACAGCAGACT GCAGAAAGAC CATGAAAACA TCCGGAAGAA GTTCGGCTGC 
GACAAAACCA CACCTCTGGA GAACCTCAAC GACATGCTGA AAAACCTGGA GAAAGAGAAG 
GAGAAGGTCA TCGAGAATAA GCGTCAGGTT CAGACGCTGG TCAGCAAGTC CAAGAACATC 
GTGTGTCTGA AACCTCGTAA TCCAGAGGAG AAGAGCAGCA GTCCGGTCAT AGTGCAAGCT 
CTGTGTGACT TCAAACAGGA TCAAAAAGGC ATTATGAAGG GCAATGAAGG TATCCTGAAA 
GACAACTCTC AGCGCAGTAA GTGGCACGTG ACTGGACCTG GAGGACTGGA AATGCTCATC 
CCGTCTGTGT GTCTGATCAT CCCACCGCCA AACCCTCTGA GCATCAGCCT GGCAAACAAG 
AACGAGCAGT ATTATGAAGC CATTATGTCC ATATGGAGTC AGCTGTACAT CAACATCAAG 
AGTCTGATAT CCTGGCAGTA CTGTCTGAGA GACATCCAGC ACATCAACTC CCTCACTCTC 
TCTATGCTGA CGCAAATGCG TCCTGAGGAG TATCGCCAAA CCATCAAGAA CCTGGAGATC 
CACTACCAGG AGTTTCTGCG CAACAGTCTT GGCTCTGAGA TGTTCGGTGA TGACGACAAG 
AGAAAGATGG AGCTTCAGTA TGCCGGAGCT CAGTCCCACT ACGACCAGCT GGTGATCCAA 
CTGCCCAACT ACAGGGAGAA TGGAGTGATC AGAGAGGTGG TGATGGTGGA GACAGATTCC 
AAGCTGAAAT CTGAGGTGTC GAGTGGGAAG ACTGCGACCG GGGTGTCGAG TGGGAAGACT 
GCGACAGGAG TGTCGAGTGG GAAGACTTCG TCTTCTGTGT CGGTTTCGGG TCTTAATGTG 
AGTCTGCTGT CGGACCTCAG CGCTCTGAGA CGGAGACTAG AGACAGCAGA ATCCGGGCTC 
ACCAAACATC TGCACGTTCC GCTGAAGGAG AACAGCGTTC AGGAGTGCTC ACAGAGACTG 
GCGCAGCTAC AGGCTGTTCA TCGTGATCTG GACTCCATCA GGGACGAGTA TCTGCATCTG 
AGGGAGAAGA TTATGAGAGA GCTGGAGGGC AGCAGTGACC CTGAGCAGAG CCGATACCTG 
CGCGCAGAAC TGGACCTGAT CAACCAGAAA CTGGGCAGCC TGCAGGGCTT CTCCAGCGCA 
TACATCCAGA GGCTCGGTGC TCTACAAGCT CTGCTCCAGC ACCTCCTGCA GGCCGAGGAC 
ATCATTAAAG TCCACGAGGC TCGTCTGACC GAGAAAGAAA CCTCTTCTCT TGACCTGAAC 
GAAGTGGAAA AGTACTGCAT GACTCTCAAG ACTATGAAAG CAGAGCTGGA GCAGAAGAAG 
GGTGTTTTGA AGGCGATGGA GACGGAGCTG TCTAAAGCTG TGCACTGGAA CAGTCAGATT 
GACCAGTCTT TCCATCAGTG TGATGTTGAC CTGTCCAGAT ACACTGAGCT GGTCGGACAG 
ATGACTGACC GCTGGCGCAG GATTGTGACT CAGATTGATA GCAGGACATG GGACCTGGAG 
AAGCAAGAGA AACAGCTGAA TCACTACCAG CAGACCAGCT CCACGATAAA CAGATGGATT 
CAGGACACAC GTCAGCGCCA AGACACCCTG CAGATTACCA AATTCAACAG CGTGGACAAT 
TTAATGGACC ACCTCAACCA GCAAAAAGCG CTTTATTCTG AGATCAAGGG GAAGAAAGAA 
AAGGTGGATG CTGTGGTCAA AGACTCAGAC ACATGTGCTG CCTCCATAAA GGACTACGAG 
CTCCAGTTGG CGTCTTATAG TGCAGGTCTG GAGACTCTGC TCAACATTCC CATCAAAAAG 
ACTATGCTGC AGTCTCCAGC CACTGTTCTC CGGGAGGAGG CAACAGATTT GCAGTCTCGC 
TACATTGAGC TCCTGACACG ATCAAGTGAC TACTACAAAT TCCTTGGGGA AATGCTGAAA 
AACATGGAGG AGTTAAAGAT GCGGAACACC AAAATTGAGC TTTTGGAGGA GGAACTGAGA 
CGCCTCAAGG ACAACCTCAA AGATCAAAAC CAGAAGAACA AATCACTGGA GGACAGTTTG 
ACTCGCTTCC GCCTGGAGCT GACCCAGTCC AAAGAGCAGC TCATATCTAT GGAGGAGGTG 
AAGAGAACCC AGGCCAGGCA GTGCAACACT GCTCAGGAAA GCCTGGATAG TACTCAGAAC 
CAGCTGAAGA GTTTACAAGA TGAGATGTCC CGCCTTACAT TCCTCATCGA GGAGGAAAAG 
CGCAAGCGAA GGCTGGCCGA GGAGCGTTAC ACCAACCAGC AGGAGGAGTA TGAGCTGGCC 
ATGCGCAAGA GACAGAAAGA ACTGGAGGAG CTCACTTTGT CCAAAAGTCA ATTTGAAAGA 
GCCATCAAAG AGAAAGAGCG GGAGATTGAA CGGCTGAAAT TACAGCTGCA GGATGAGGCC 
TCCCGACGCT CGGCAGCAGA ACTGGAGACC TCAAAGGTAA GAACCCAGTT GAACCAGGAC 
ATAAGTAGCC TAAAGCAAAC ATATGAATCT GAGATCCACG TCACCAAGAC CACAGTGCTC 
AAGTCGGCAC AGCAGAAGGA TGAGGAATCG GCATCTCTGA GACTTCAGGT GGACAGGTTA 
ACCTCTGAGA AGAGAGATCT GGAAGAGGAG CTAAGGAAAC TGCAGCTTTC TTTCTCCCAG 
ACGGAAGCGG CACGACGGAA AGCAGAAGAG GACGCGCATC AGAAGAGATC TATGGCTGCA 
GATGAAAGCA GATCCAAGAA GGAACTGGAG TCACAGATTC AGATCATAAT TCGTCAAAGG 
GAAGACATTG AAACTAGACA CAAGATGGAC CTGGCTGATG CAAACAGAGT TGCTCAGGAA 
AAGACCCGTG AGATCACATT GCTGACGCAA AATTTGCAAG ACGAGACCAG GCGGAGGAAA 
GCAATGGAGG TTGAGAATCA AAGCCTCAGA CAGTCTCAGG CTGAAATACT AGCCAAGCAG 
ACTTCAACCA CAGAGGTCAT CAACAAATTA AAGGTCTCTG AGCAAGAGGT TCTCATTATA 
AAGAGAGAGT TGGATGTACA GAAAAGTGAT AAGATGAAGT TGGAGCAGAA TGCCACCAGG 
TTGCAGAGTC GCATAAATGA ACTGCAGTCC AAGGTGAACG AGTTGCAGGC TGAACTGGAG 
AAAGAGAGAA GGAATAATCA GGATGAGCTC ACGAGGAGGA AACGCATGGA AACTGAGTTG 
GATAGGGTAA ATCAGACGTG CCGTGAATAC ACCACTACCA TCAGCACTCT GCGGGTCCAA 
CAAGAGAAAG AAAGTTCTTC TGGACGGAGG TACGAGCAGG AGCTTCGCAG TGCAAAAGAC 
GAGTTGGAGA GAAGCCTGAA AGAGTATAAA ATCACAGTAG AGAATCTGAC CAAAGTCAAT 
GCAGAACTGA AGGCTTTGCA ACAGCAGCTT GTACGGGAGC AGGGCCTGGT CCGAGAGGCT 
AATCAGCGAA ATGATTCCCT CTACAAGACT ATTGAGGAGA AGAGTCGACT ACTAAATGAG 
AGCACTAGCG AGGTTGAAAA GTTGCAGACC CTCACCCAGA ATCTTACCAA AGAGCGCCTG 
CGACTTGAGG AGGAACTAAG GAATGTGCGG CTCGAGCGGG ATGATGCTAA AAGAAGTCTG 
GGCACCATTG AGAGTGAAAG TGCTTCACGA TTGTCTGCCA TTCAGTTTCA GTTACAAACC 
AGCAATAACA GAGCAGTGGA ACTGCAGGAG CTAATCAATG AACTGACAAA AGAGAGGGAA 
AGTTTAAGGG TGGAAATCGC CCAAATCCAA AAGCAGTTCT CAGAGACATC CATGATGATC 
CAGCGATCTG ATTCAAACTA CAAAGACATT GTGCAAGAGA GAGATAGTCT CTTAATCAAA 
CTGAAGCTGC TGCAACAGGA CAAAGATAAG CAGCAGCGAT ATGAAGAGGA GCTCAGGCGC 
ATCAAACTTA CCTTGGAATC AGAGACCAAA CAGAAGCAAC GTCTCCAGGA TGAAATCGAT 
AAAATCACCA AGGATTTCAA GTACTGGAAA AGCCAGTACG AGTTAAAAGA GGGGCAGATC 
AGACAGAGCG AGATGGATAG AGACAGAGTA GAGAGAGACC GAGCCTCCTT ACAGAGTGAG 
ATCCAAAGAT TGACCGCTGA GCTGAGAAGT GTTGAGGAAC GCTACAGAGG TCGTCTTCAG 
AGCTCAGACA AGGAAATCTC GGAGCTAATG CGTAAGAAGG AGTCTTTGGA AATTGAGCTG 
AGGAGATTGC AACAGCGTCC AGCTGCAACC TGGAAGCAAA CCCAGACAGA TGAGATCTCT 
AAGCCAGTTG TTGAGCAGAA ATTGACAGTG CAAGGTCTTC GTGGTGAGGT CTCACTTACA 
GAGCTTGTCG AGTCCGACTT GCTCGATCAA ACTGATTTGG ACAAAATAAA CCGAGGACAG 
CTAACCAGTA AAGATATCGA GCACAAGCTC AAATCATACC TTGGTGGCTC TGATTGTATC 
GCTGGCATCT ATGATGAGGC CAAGGATAGA GTCATGCCTT TTTACCAGGC GATGAAAGAT 
GGTCTACTCC GACGAGGAAC CACACTGGAG CTCCTGGAGG CCCAGGCTGC CTCTGGTTTC 
ATAATTGACC CAGTTAACAA TGTCTGTATG ACCGTTGAAG AAGCTTGGAA GAGAGGTCTT 
GTAGGAAAGG AGTTCAAAGA CAAACTCCTG TCTGCTGAGA AGGCTGTGAC GGGATACAAA 
GATCCTGCAA CAGGAAAGAT CATCTCACTC TTCCAAGCTA TTGAGAAAGA GATCATTGAG 
AAAGGCCATG GAATCAGACT GCTGGAAGCT CAGATAGCCA GCGGTGGGAT TATTGATCCC 
AAAGGTAGCC ATCGAATTGA TGTGGAAGTA GCTTACAGGA AGGGTTACTT TGACCGTGAG 
ATGAATGAGA TTTTGTCCTA TGAAGGGGAT GACACCAAAG GTTTCTTTGA TCCCAACACC 
CATGAAAACC TCACATATCT AGAGTTGAAA AAGAGGTGCA TTAAAGATCC CAAGACTGGG 
CTCATGCTTT TGCCTTTAAA TGACAAGCAG AAACCAAAGC AGACGACAAC CCAGAAGAAC 
ACACTACGCA AAAGAAGAGT AGTGATCGTA GACCCTGACA CTGGTAAAGA GATGACTGTA 
CGAGAAGCGT ACCATCGAGA GTTAATCGAC TATGACACCT TTCTGGAACT TTCCGAACAA 
GAGTGCGAGT GGGAGGAGAT CACGATTGAA ACATCTGATG GAAACAAGCG TTTACTTATT 
GTCGATCGCA AAACTGGAAT CCAGTATGAC ATTCAGGAGT CCCTACAGAG GGGAATTATC 
AATAAACAAA CTTTGGAAAA GTACCGTGCA GGGACTATGA CGCTCACCGA GTTTGCAGCT 
CTGATCACTA GTAAAAGCAA CAGCTCTGAG CTTGCCATCT TCTCCAGCAG TCCTGAGGAT 
GTAGCCACCT GCAGCAGTCC CACACAGCCA TCATCCCCAA CCGTCCGCAA ACGATTTGCA 
AGTGTTTCCA TAACTCTATC CCCTCCATCT GATATTTTTG ACGACCAGAG CCCTGTGGGA 
GCAATATTCG ATACGGAGAC CCTTGAGAAG ATAACCATCC CCGAGGCTCA GCGACGTGGA 
ATAGTGGACA ACATCACCGC TCAGCGTCTT CTTGAAGCGC AGGTTTGCTC CGGAGGAATT 
ATCAATCCCG CTACAGGCCA GAGACTATCT CTGAAAGACG CCGTACAGCA AAGCCTCATC 
GATGAAGACA TGAGCGTCAA GCTAAAACCA GCGCAGAAGG CTTATGACGG TTTTGAGGAT 
GTGAAGACCA AGAGGAAACT TTCTGCTGCC GAAGCCATGA AGGAAAAATG GTTGCCCTAC 
GAAGCCGGCC AGAGGTTTCT AGAGTTCCAG TATCTCACAG GTGGACTCAT AGAGCCAGGA 
ACTGGACGCC GTGTCAGCAT TGAGGAGGCC ATCCGGAAAG GGTGGCTTGA TGGCAAAGGT 
GCACAAAAGC TGCAAGATAC CAGGAACTAC ATTAAGAACC TTACCTGTCC CAAAACCAAG 
CTTAAAATCT CATACAAAGA GGCGATGGAT AACTGCATGG TGGAGGAGAA CAATGGCATG 
AAGATGCTCC AGGCGACCTC CATGTCTACC AAAGGCATAA GCAGCCCCTA TAATGTGTCC 
TCTGGGCCAA GCTCTCGCTC TGGATCCAGA GCCGGATCCC GAACAGGCTC AAGAAGTGGA 
TCGCGCAGAG GCAGTGTCGA CTACAGCTCT TCATCTGTTT CTTACACCTT CTTCTCCTCT 
GCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dspa ( XM_005171104.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171161.1 Danio rerio desmoplakin a (dspa), transcript variant X2, mRNA.

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CloneID	ODa06596
Clone ID Related Accession (Same CDS sequence)	XM_005171104.3 , XM_005171104.4
Accession Version	XM_005171104.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	desmoplakin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGT CCGGTTCTCA GACCAGGCTC CACCAGATCA GCCGCAGGTC CAGTTCGCGG 
CCAGACCTCA CCGCCGTCGG CTACAGTTTA CCGAGGAACG ACGGAGCTCA GTTTTTCACG 
AGTGGAAACG GATACCAGTC CGACTATAAT GACGGCTACA TGCAGACCTA CACGCAGACT 
TTCTCCAAAA ACTCTCAGGG TGGAGGAGCT GGAGGAGCCC AGAGCATGTC TTCCATGGCG 
GTTCAGAAGC GAGCTCAGAT GTTGTACAGT GACTGCATGG GATTCCTGCA GCGAGCGCAG 
GGCCTCCTGG AGACTATGGG ACCTGCTTCT GAGGTGGACA AGAACATGAT GGCAGCGCAG 
GACGCCATGG ATCAGCTGCG GAAATGTGCG GTGGACATGA GGAACGCCGG TCTGCCTAAT 
GACATGATCC TCCGCAGTTT GGAGGACTGT CATAGCTTTT ATGCAGACAT TCGCAACTCC 
ATCACGGGCA CCACCATCAG GAGGACTGGC GGCACCGTCA GCGGCACCAT CGGTGGCACC 
ATCAGTGGCA CCATTGGTGG CACTGTCGGC GGCTGGGACG ATCCCAGCAA GAGCTTTCAG 
GACGCACTGT CATGGATCAA CCAGAAGAAG AGACTCATTG AAACGTCAGG CTTCGGTGAA 
AATTCAGAAG CCATTAGCCA ACAAATCCTG AATCACAACA AGTTCCACAG TTCAATCCAG 
AGAAGCCAGG AGGTGGACAG AGCCCGCGAC GATCTGATGC AGAGCAGGGA AAAGGCTGGA 
CTACATGCTC TGGACCAAGA GTGGGACAGT TTACAGAAAA TGTCCCATGC ACGTATGGAT 
CATCTGCGAG ACCTACAGAG CATCATCGAG GAGATTTCCA GAGCCATCAT GTGGGTCAAC 
GACAGAGAGG AGGAGGAGCT GGTGTTTGAC TGGGGCGATA AAAACATTGA CAACTACATC 
CCTCAAAAAC AAGAGAGCTA CTCGAAACTG ATGAGTGATC TGGAGGAGAA GGAGAAAGAG 
CTGAATAAGC TAAAGGCAAA GGTGGACATG CTGCTGAAGA ACCAGCACCC GGCATCTGAT 
AAGATAGAGG CTTACATGGA CACACTGCAA ACCCAGTGGA GCTGGTTACT GCAGATCACC 
AAGTGCATCC ATGTTCACCT GAAGGAGAAC GCAGCCTACA GCCAGTTCTT CAAGGAGGCC 
AATGAGACGT ACAGCAGACT GCAGAAAGAC CATGAAAACA TCCGGAAGAA GTTCGGCTGC 
GACAAAACCA CACCTCTGGA GAACCTCAAC GACATGCTGA AAAACCTGGA GAAAGAGAAG 
GAGAAGGTCA TCGAGAATAA GCGTCAGGTT CAGACGCTGG TCAGCAAGTC CAAGAACATC 
GTGTGTCTGA AACCTCGTAA TCCAGAGGAG AAGAGCAGCA GTCCGGTCAT AGTGCAAGCT 
CTGTGTGACT TCAAACAGGA TCAAAAAGGC ATTATGAAGG GCAATGAAGG TATCCTGAAA 
GACAACTCTC AGCGCAGTAA GTGGCACGTG ACTGGACCTG GAGGACTGGA AATGCTCATC 
CCGTCTGTGT GTCTGATCAT CCCACCGCCA AACCCTCTGA GCATCAGCCT GGCAAACAAG 
AACGAGCAGT ATTATGAAGC CATTATGTCC ATATGGAGTC AGCTGTACAT CAACATCAAG 
AGTCTGATAT CCTGGCAGTA CTGTCTGAGA GACATCCAGC ACATCAACTC CCTCACTCTC 
TCTATGCTGA CGCAAATGCG TCCTGAGGAG TATCGCCAAA CCATCAAGAA CCTGGAGATC 
CACTACCAGG AGTTTCTGCG CAACAGTCTT GGCTCTGAGA TGTTCGGTGA TGACGACAAG 
AGAAAGATGG AGCTTCAGTA TGCCGGAGCT CAGTCCCACT ACGACCAGCT GGTGATCCAA 
CTGCCCAACT ACAGGGAGAA TGGAGTGATC AGAGAGGTGG TGATGGTGGA GACAGATTCC 
AAGCTGAAAT CTGAGGTGTC GAGTGGGAAG ACTGCGACCG GGGTGTCGAG TGGGAAGACT 
GCGACAGGAG TGTCGAGTGG GAAGACTTCG TCTTCTGTGT CGGTTTCGGG TCTTAATGTG 
AGTCTGCTGT CGGACCTCAG CGCTCTGAGA CGGAGACTAG AGACAGCAGA ATCCGGGCTC 
ACCAAACATC TGCACGTTCC GCTGAAGGAG AACAGCGTTC AGGAGTGCTC ACAGAGACTG 
GCGCAGCTAC AGGCTGTTCA TCGTGATCTG GACTCCATCA GGGACGAGTA TCTGCATCTG 
AGGGAGAAGA TTATGAGAGA GCTGGAGGGC AGCAGTGACC CTGAGCAGAG CCGATACCTG 
CGCGCAGAAC TGGACCTGAT CAACCAGAAA CTGGGCAGCC TGCAGGGCTT CTCCAGCGCA 
TACATCCAGA GGCTCGGTGC TCTACAAGCT CTGCTCCAGC ACCTCCTGCA GGCCGAGGAC 
ATCATTAAAG TCCACGAGGC TCGTCTGACC GAGAAAGAAA CCTCTTCTCT TGACCTGAAC 
GAAGTGGAAA AGTACTGCAT GACTCTCAAG ACTATGAAAG CAGAGCTGGA GCAGAAGAAG 
GGTGTTTTGA AGGCGATGGA GACGGAGCTG TCTAAAGCTG TGCACTGGAA CAGTCAGATT 
GACCAGTCTT TCCATCAGTG TGATGTTGAC CTGTCCAGAT ACACTGAGCT GGTCGGACAG 
ATGACTGACC GCTGGCGCAG GATTGTGACT CAGATTGATA GCAGGACATG GGACCTGGAG 
AAGCAAGAGA AACAGCTGAA TCACTACCAG CAGACCAGCT CCACGATAAA CAGATGGATT 
CAGGACACAC GTCAGCGCCA AGACACCCTG CAGATTACCA AATTCAACAG CGTGGACAAT 
TTAATGGACC ACCTCAACCA GCAAAAAGCG CTTTATTCTG AGATCAAGGG GAAGAAAGAA 
AAGGTGGATG CTGTGGTCAA AGACTCAGAC ACATGTGCTG CCTCCATAAA GGACTACGAG 
CTCCAGTTGG CGTCTTATAG TGCAGGTCTG GAGACTCTGC TCAACATTCC CATCAAAAAG 
ACTATGCTGC AGTCTCCAGC CACTGTTCTC CGGGAGGAGG CAACAGATTT GCAGTCTCGC 
TACATTGAGC TCCTGACACG ATCAAGTGAC TACTACAAAT TCCTTGGGGA AATGCTGAAA 
AACATGGAGG AGTTAAAGAT GCGGAACACC AAAATTGAGC TTTTGGAGGA GGAACTGAGA 
CGCCTCAAGG ACAACCTCAA AGATCAAAAC CAGAAGAACA AATCACTGGA GGACAGTTTG 
ACTCGCTTCC GCCTGGAGCT GACCCAGTCC AAAGAGCAGC TCATATCTAT GGAGGAGGTG 
AAGAGAACCC AGGCCAGGCA GTGCAACACT GCTCAGGAAA GCCTGGATAG TACTCAGAAC 
CAGCTGAAGA GTTTACAAGA TGAGATGTCC CGCCTTACAT TCCTCATCGA GGAGGAAAAG 
CGCAAGCGAA GGCTGGCCGA GGAGCGTTAC ACCAACCAGC AGGAGGAGTA TGAGCTGGCC 
ATGCGCAAGA GACAGAAAGA ACTGGAGGAG CTCACTTTGT CCAAAAGTCA ATTTGAAAGA 
GCCATCAAAG AGAAAGAGCG GGAGATTGAA CGGCTGAAAT TACAGCTGCA GGATGAGGCC 
TCCCGACGCT CGGCAGCAGA ACTGGAGACC TCAAAGACAT CCATGATGAT CCAGCGATCT 
GATTCAAACT ACAAAGACAT TGTGCAAGAG AGAGATAGTC TCTTAATCAA ACTGAAGCTG 
CTGCAACAGG ACAAAGATAA GCAGCAGCGA TATGAAGAGG AGCTCAGGCG CATCAAACTT 
ACCTTGGAAT CAGAGACCAA ACAGAAGCAA CGTCTCCAGG ATGAAATCGA TAAAATCACC 
AAGGATTTCA AGTACTGGAA AAGCCAGTAC GAGTTAAAAG AGGGGCAGAT CAGACAGAGC 
GAGATGGATA GAGACAGAGT AGAGAGAGAC CGAGCCTCCT TACAGAGTGA GATCCAAAGA 
TTGACCGCTG AGCTGAGAAG TGTTGAGGAA CGCTACAGAG GTCGTCTTCA GAGCTCAGAC 
AAGGAAATCT CGGAGCTAAT GCGTAAGAAG GAGTCTTTGG AAATTGAGCT GAGGAGATTG 
CAACAGCGTC CAGCTGCAAC CTGGAAGCAA ACCCAGACAG ATGAGATCTC TAAGCCAGTT 
GTTGAGCAGA AATTGACAGT GCAAGGTCTT CGTGGTGAGG TCTCACTTAC AGAGCTTGTC 
GAGTCCGACT TGCTCGATCA AACTGATTTG GACAAAATAA ACCGAGGACA GCTAACCAGT 
AAAGATATCG AGCACAAGCT CAAATCATAC CTTGGTGGCT CTGATTGTAT CGCTGGCATC 
TATGATGAGG CCAAGGATAG AGTCATGCCT TTTTACCAGG CGATGAAAGA TGGTCTACTC 
CGACGAGGAA CCACACTGGA GCTCCTGGAG GCCCAGGCTG CCTCTGGTTT CATAATTGAC 
CCAGTTAACA ATGTCTGTAT GACCGTTGAA GAAGCTTGGA AGAGAGGTCT TGTAGGAAAG 
GAGTTCAAAG ACAAACTCCT GTCTGCTGAG AAGGCTGTGA CGGGATACAA AGATCCTGCA 
ACAGGAAAGA TCATCTCACT CTTCCAAGCT ATTGAGAAAG AGATCATTGA GAAAGGCCAT 
GGAATCAGAC TGCTGGAAGC TCAGATAGCC AGCGGTGGGA TTATTGATCC CAAAGGTAGC 
CATCGAATTG ATGTGGAAGT AGCTTACAGG AAGGGTTACT TTGACCGTGA GATGAATGAG 
ATTTTGTCCT ATGAAGGGGA TGACACCAAA GGTTTCTTTG ATCCCAACAC CCATGAAAAC 
CTCACATATC TAGAGTTGAA AAAGAGGTGC ATTAAAGATC CCAAGACTGG GCTCATGCTT 
TTGCCTTTAA ATGACAAGCA GAAACCAAAG CAGACGACAA CCCAGAAGAA CACACTACGC 
AAAAGAAGAG TAGTGATCGT AGACCCTGAC ACTGGTAAAG AGATGACTGT ACGAGAAGCG 
TACCATCGAG AGTTAATCGA CTATGACACC TTTCTGGAAC TTTCCGAACA AGAGTGCGAG 
TGGGAGGAGA TCACGATTGA AACATCTGAT GGAAACAAGC GTTTACTTAT TGTCGATCGC 
AAAACTGGAA TCCAGTATGA CATTCAGGAG TCCCTACAGA GGGGAATTAT CAATAAACAA 
ACTTTGGAAA AGTACCGTGC AGGGACTATG ACGCTCACCG AGTTTGCAGC TCTGATCACT 
AGTAAAAGCA ACAGCTCTGA GCTTGCCATC TTCTCCAGCA GTCCTGAGGA TGTAGCCACC 
TGCAGCAGTC CCACACAGCC ATCATCCCCA ACCGTCCGCA AACGATTTGC AAGTGTTTCC 
ATAACTCTAT CCCCTCCATC TGATATTTTT GACGACCAGA GCCCTGTGGG AGCAATATTC 
GATACGGAGA CCCTTGAGAA GATAACCATC CCCGAGGCTC AGCGACGTGG AATAGTGGAC 
AACATCACCG CTCAGCGTCT TCTTGAAGCG CAGGTTTGCT CCGGAGGAAT TATCAATCCC 
GCTACAGGCC AGAGACTATC TCTGAAAGAC GCCGTACAGC AAAGCCTCAT CGATGAAGAC 
ATGAGCGTCA AGCTAAAACC AGCGCAGAAG GCTTATGACG GTTTTGAGGA TGTGAAGACC 
AAGAGGAAAC TTTCTGCTGC CGAAGCCATG AAGGAAAAAT GGTTGCCCTA CGAAGCCGGC 
CAGAGGTTTC TAGAGTTCCA GTATCTCACA GGTGGACTCA TAGAGCCAGG AACTGGACGC 
CGTGTCAGCA TTGAGGAGGC CATCCGGAAA GGGTGGCTTG ATGGCAAAGG TGCACAAAAG 
CTGCAAGATA CCAGGAACTA CATTAAGAAC CTTACCTGTC CCAAAACCAA GCTTAAAATC 
TCATACAAAG AGGCGATGGA TAACTGCATG GTGGAGGAGA ACAATGGCAT GAAGATGCTC 
CAGGCGACCT CCATGTCTAC CAAAGGCATA AGCAGCCCCT ATAATGTGTC CTCTGGGCCA 
AGCTCTCGCT CTGGATCCAG AGCCGGATCC CGAACAGGCT CAAGAAGTGG ATCGCGCAGA 
GGCAGTGTCG ACTACAGCTC TTCATCTGTT TCTTACACCT TCTTCTCCTC TGCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171161.1
CDS	133..6312
Translation	MSLSGSQTRLHQISRRSSSRPDLTAVGYSLPRNDGAQFFTSGNGYQSDYNDGYMQTYTQTFSKNSQGGGAGGAQSMSSMAVQKRAQMLYSDCMGFLQRAQGLLETMGPASEVDKNMMAAQDAMDQLRKCAVDMRNAGLPNDMILRSLEDCHSFYADIRNSITGTTIRRTGGTVSGTIGGTISGTIGGTVGGWDDPSKSFQDALSWINQKKRLIETSGFGENSEAISQQILNHNKFHSSIQRSQEVDRARDDLMQSREKAGLHALDQEWDSLQKMSHARMDHLRDLQSIIEEISRAIMWVNDREEEELVFDWGDKNIDNYIPQKQESYSKLMSDLEEKEKELNKLKAKVDMLLKNQHPASDKIEAYMDTLQTQWSWLLQITKCIHVHLKENAAYSQFFKEANETYSRLQKDHENIRKKFGCDKTTPLENLNDMLKNLEKEKEKVIENKRQVQTLVSKSKNIVCLKPRNPEEKSSSPVIVQALCDFKQDQKGIMKGNEGILKDNSQRSKWHVTGPGGLEMLIPSVCLIIPPPNPLSISLANKNEQYYEAIMSIWSQLYINIKSLISWQYCLRDIQHINSLTLSMLTQMRPEEYRQTIKNLEIHYQEFLRNSLGSEMFGDDDKRKMELQYAGAQSHYDQLVIQLPNYRENGVIREVVMVETDSKLKSEVSSGKTATGVSSGKTATGVSSGKTSSSVSVSGLNVSLLSDLSALRRRLETAESGLTKHLHVPLKENSVQECSQRLAQLQAVHRDLDSIRDEYLHLREKIMRELEGSSDPEQSRYLRAELDLINQKLGSLQGFSSAYIQRLGALQALLQHLLQAEDIIKVHEARLTEKETSSLDLNEVEKYCMTLKTMKAELEQKKGVLKAMETELSKAVHWNSQIDQSFHQCDVDLSRYTELVGQMTDRWRRIVTQIDSRTWDLEKQEKQLNHYQQTSSTINRWIQDTRQRQDTLQITKFNSVDNLMDHLNQQKALYSEIKGKKEKVDAVVKDSDTCAASIKDYELQLASYSAGLETLLNIPIKKTMLQSPATVLREEATDLQSRYIELLTRSSDYYKFLGEMLKNMEELKMRNTKIELLEEELRRLKDNLKDQNQKNKSLEDSLTRFRLELTQSKEQLISMEEVKRTQARQCNTAQESLDSTQNQLKSLQDEMSRLTFLIEEEKRKRRLAEERYTNQQEEYELAMRKRQKELEELTLSKSQFERAIKEKEREIERLKLQLQDEASRRSAAELETSKTSMMIQRSDSNYKDIVQERDSLLIKLKLLQQDKDKQQRYEEELRRIKLTLESETKQKQRLQDEIDKITKDFKYWKSQYELKEGQIRQSEMDRDRVERDRASLQSEIQRLTAELRSVEERYRGRLQSSDKEISELMRKKESLEIELRRLQQRPAATWKQTQTDEISKPVVEQKLTVQGLRGEVSLTELVESDLLDQTDLDKINRGQLTSKDIEHKLKSYLGGSDCIAGIYDEAKDRVMPFYQAMKDGLLRRGTTLELLEAQAASGFIIDPVNNVCMTVEEAWKRGLVGKEFKDKLLSAEKAVTGYKDPATGKIISLFQAIEKEIIEKGHGIRLLEAQIASGGIIDPKGSHRIDVEVAYRKGYFDREMNEILSYEGDDTKGFFDPNTHENLTYLELKKRCIKDPKTGLMLLPLNDKQKPKQTTTQKNTLRKRRVVIVDPDTGKEMTVREAYHRELIDYDTFLELSEQECEWEEITIETSDGNKRLLIVDRKTGIQYDIQESLQRGIINKQTLEKYRAGTMTLTEFAALITSKSNSSELAIFSSSPEDVATCSSPTQPSSPTVRKRFASVSITLSPPSDIFDDQSPVGAIFDTETLEKITIPEAQRRGIVDNITAQRLLEAQVCSGGIINPATGQRLSLKDAVQQSLIDEDMSVKLKPAQKAYDGFEDVKTKRKLSAAEAMKEKWLPYEAGQRFLEFQYLTGGLIEPGTGRRVSIEEAIRKGWLDGKGAQKLQDTRNYIKNLTCPKTKLKISYKEAMDNCMVEENNGMKMLQATSMSTKGISSPYNVSSGPSSRSGSRAGSRTGSRSGSRRGSVDYSSSSVSYTFFSSAS

Target ORF information:

RefSeq Version	XM_005171104.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio desmoplakin a (dspa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171104.3

ORF Insert Sequence:

ATGAGTTTGT CCGGTTCTCA GACCAGGCTC CACCAGATCA GCCGCAGGTC CAGTTCGCGG 
CCAGACCTCA CCGCCGTCGG CTACAGTTTA CCGAGGAACG ACGGAGCTCA GTTTTTCACG 
AGTGGAAACG GATACCAGTC CGACTATAAT GACGGCTACA TGCAGACCTA CACGCAGACT 
TTCTCCAAAA ACTCTCAGGG TGGAGGAGCT GGAGGAGCCC AGAGCATGTC TTCCATGGCG 
GTTCAGAAGC GAGCTCAGAT GTTGTACAGT GACTGCATGG GATTCCTGCA GCGAGCGCAG 
GGCCTCCTGG AGACTATGGG ACCTGCTTCT GAGGTGGACA AGAACATGAT GGCAGCGCAG 
GACGCCATGG ATCAGCTGCG GAAATGTGCG GTGGACATGA GGAACGCCGG TCTGCCTAAT 
GACATGATCC TCCGCAGTTT GGAGGACTGT CATAGCTTTT ATGCAGACAT TCGCAACTCC 
ATCACGGGCA CCACCATCAG GAGGACTGGC GGCACCGTCA GCGGCACCAT CGGTGGCACC 
ATCAGTGGCA CCATTGGTGG CACTGTCGGC GGCTGGGACG ATCCCAGCAA GAGCTTTCAG 
GACGCACTGT CATGGATCAA CCAGAAGAAG AGACTCATTG AAACGTCAGG CTTCGGTGAA 
AATTCAGAAG CCATTAGCCA ACAAATCCTG AATCACAACA AGTTCCACAG TTCAATCCAG 
AGAAGCCAGG AGGTGGACAG AGCCCGCGAC GATCTGATGC AGAGCAGGGA AAAGGCTGGA 
CTACATGCTC TGGACCAAGA GTGGGACAGT TTACAGAAAA TGTCCCATGC ACGTATGGAT 
CATCTGCGAG ACCTACAGAG CATCATCGAG GAGATTTCCA GAGCCATCAT GTGGGTCAAC 
GACAGAGAGG AGGAGGAGCT GGTGTTTGAC TGGGGCGATA AAAACATTGA CAACTACATC 
CCTCAAAAAC AAGAGAGCTA CTCGAAACTG ATGAGTGATC TGGAGGAGAA GGAGAAAGAG 
CTGAATAAGC TAAAGGCAAA GGTGGACATG CTGCTGAAGA ACCAGCACCC GGCATCTGAT 
AAGATAGAGG CTTACATGGA CACACTGCAA ACCCAGTGGA GCTGGTTACT GCAGATCACC 
AAGTGCATCC ATGTTCACCT GAAGGAGAAC GCAGCCTACA GCCAGTTCTT CAAGGAGGCC 
AATGAGACGT ACAGCAGACT GCAGAAAGAC CATGAAAACA TCCGGAAGAA GTTCGGCTGC 
GACAAAACCA CACCTCTGGA GAACCTCAAC GACATGCTGA AAAACCTGGA GAAAGAGAAG 
GAGAAGGTCA TCGAGAATAA GCGTCAGGTT CAGACGCTGG TCAGCAAGTC CAAGAACATC 
GTGTGTCTGA AACCTCGTAA TCCAGAGGAG AAGAGCAGCA GTCCGGTCAT AGTGCAAGCT 
CTGTGTGACT TCAAACAGGA TCAAAAAGGC ATTATGAAGG GCAATGAAGG TATCCTGAAA 
GACAACTCTC AGCGCAGTAA GTGGCACGTG ACTGGACCTG GAGGACTGGA AATGCTCATC 
CCGTCTGTGT GTCTGATCAT CCCACCGCCA AACCCTCTGA GCATCAGCCT GGCAAACAAG 
AACGAGCAGT ATTATGAAGC CATTATGTCC ATATGGAGTC AGCTGTACAT CAACATCAAG 
AGTCTGATAT CCTGGCAGTA CTGTCTGAGA GACATCCAGC ACATCAACTC CCTCACTCTC 
TCTATGCTGA CGCAAATGCG TCCTGAGGAG TATCGCCAAA CCATCAAGAA CCTGGAGATC 
CACTACCAGG AGTTTCTGCG CAACAGTCTT GGCTCTGAGA TGTTCGGTGA TGACGACAAG 
AGAAAGATGG AGCTTCAGTA TGCCGGAGCT CAGTCCCACT ACGACCAGCT GGTGATCCAA 
CTGCCCAACT ACAGGGAGAA TGGAGTGATC AGAGAGGTGG TGATGGTGGA GACAGATTCC 
AAGCTGAAAT CTGAGGTGTC GAGTGGGAAG ACTGCGACCG GGGTGTCGAG TGGGAAGACT 
GCGACAGGAG TGTCGAGTGG GAAGACTTCG TCTTCTGTGT CGGTTTCGGG TCTTAATGTG 
AGTCTGCTGT CGGACCTCAG CGCTCTGAGA CGGAGACTAG AGACAGCAGA ATCCGGGCTC 
ACCAAACATC TGCACGTTCC GCTGAAGGAG AACAGCGTTC AGGAGTGCTC ACAGAGACTG 
GCGCAGCTAC AGGCTGTTCA TCGTGATCTG GACTCCATCA GGGACGAGTA TCTGCATCTG 
AGGGAGAAGA TTATGAGAGA GCTGGAGGGC AGCAGTGACC CTGAGCAGAG CCGATACCTG 
CGCGCAGAAC TGGACCTGAT CAACCAGAAA CTGGGCAGCC TGCAGGGCTT CTCCAGCGCA 
TACATCCAGA GGCTCGGTGC TCTACAAGCT CTGCTCCAGC ACCTCCTGCA GGCCGAGGAC 
ATCATTAAAG TCCACGAGGC TCGTCTGACC GAGAAAGAAA CCTCTTCTCT TGACCTGAAC 
GAAGTGGAAA AGTACTGCAT GACTCTCAAG ACTATGAAAG CAGAGCTGGA GCAGAAGAAG 
GGTGTTTTGA AGGCGATGGA GACGGAGCTG TCTAAAGCTG TGCACTGGAA CAGTCAGATT 
GACCAGTCTT TCCATCAGTG TGATGTTGAC CTGTCCAGAT ACACTGAGCT GGTCGGACAG 
ATGACTGACC GCTGGCGCAG GATTGTGACT CAGATTGATA GCAGGACATG GGACCTGGAG 
AAGCAAGAGA AACAGCTGAA TCACTACCAG CAGACCAGCT CCACGATAAA CAGATGGATT 
CAGGACACAC GTCAGCGCCA AGACACCCTG CAGATTACCA AATTCAACAG CGTGGACAAT 
TTAATGGACC ACCTCAACCA GCAAAAAGCG CTTTATTCTG AGATCAAGGG GAAGAAAGAA 
AAGGTGGATG CTGTGGTCAA AGACTCAGAC ACATGTGCTG CCTCCATAAA GGACTACGAG 
CTCCAGTTGG CGTCTTATAG TGCAGGTCTG GAGACTCTGC TCAACATTCC CATCAAAAAG 
ACTATGCTGC AGTCTCCAGC CACTGTTCTC CGGGAGGAGG CAACAGATTT GCAGTCTCGC 
TACATTGAGC TCCTGACACG ATCAAGTGAC TACTACAAAT TCCTTGGGGA AATGCTGAAA 
AACATGGAGG AGTTAAAGAT GCGGAACACC AAAATTGAGC TTTTGGAGGA GGAACTGAGA 
CGCCTCAAGG ACAACCTCAA AGATCAAAAC CAGAAGAACA AATCACTGGA GGACAGTTTG 
ACTCGCTTCC GCCTGGAGCT GACCCAGTCC AAAGAGCAGC TCATATCTAT GGAGGAGGTG 
AAGAGAACCC AGGCCAGGCA GTGCAACACT GCTCAGGAAA GCCTGGATAG TACTCAGAAC 
CAGCTGAAGA GTTTACAAGA TGAGATGTCC CGCCTTACAT TCCTCATCGA GGAGGAAAAG 
CGCAAGCGAA GGCTGGCCGA GGAGCGTTAC ACCAACCAGC AGGAGGAGTA TGAGCTGGCC 
ATGCGCAAGA GACAGAAAGA ACTGGAGGAG CTCACTTTGT CCAAAAGTCA ATTTGAAAGA 
GCCATCAAAG AGAAAGAGCG GGAGATTGAA CGGCTGAAAT TACAGCTGCA GGATGAGGCC 
TCCCGACGCT CGGCAGCAGA ACTGGAGACC TCAAAGACAT CCATGATGAT CCAGCGATCT 
GATTCAAACT ACAAAGACAT TGTGCAAGAG AGAGATAGTC TCTTAATCAA ACTGAAGCTG 
CTGCAACAGG ACAAAGATAA GCAGCAGCGA TATGAAGAGG AGCTCAGGCG CATCAAACTT 
ACCTTGGAAT CAGAGACCAA ACAGAAGCAA CGTCTCCAGG ATGAAATCGA TAAAATCACC 
AAGGATTTCA AGTACTGGAA AAGCCAGTAC GAGTTAAAAG AGGGGCAGAT CAGACAGAGC 
GAGATGGATA GAGACAGAGT AGAGAGAGAC CGAGCCTCCT TACAGAGTGA GATCCAAAGA 
TTGACCGCTG AGCTGAGAAG TGTTGAGGAA CGCTACAGAG GTCGTCTTCA GAGCTCAGAC 
AAGGAAATCT CGGAGCTAAT GCGTAAGAAG GAGTCTTTGG AAATTGAGCT GAGGAGATTG 
CAACAGCGTC CAGCTGCAAC CTGGAAGCAA ACCCAGACAG ATGAGATCTC TAAGCCAGTT 
GTTGAGCAGA AATTGACAGT GCAAGGTCTT CGTGGTGAGG TCTCACTTAC AGAGCTTGTC 
GAGTCCGACT TGCTCGATCA AACTGATTTG GACAAAATAA ACCGAGGACA GCTAACCAGT 
AAAGATATCG AGCACAAGCT CAAATCATAC CTTGGTGGCT CTGATTGTAT CGCTGGCATC 
TATGATGAGG CCAAGGATAG AGTCATGCCT TTTTACCAGG CGATGAAAGA TGGTCTACTC 
CGACGAGGAA CCACACTGGA GCTCCTGGAG GCCCAGGCTG CCTCTGGTTT CATAATTGAC 
CCAGTTAACA ATGTCTGTAT GACCGTTGAA GAAGCTTGGA AGAGAGGTCT TGTAGGAAAG 
GAGTTCAAAG ACAAACTCCT GTCTGCTGAG AAGGCTGTGA CGGGATACAA AGATCCTGCA 
ACAGGAAAGA TCATCTCACT CTTCCAAGCT ATTGAGAAAG AGATCATTGA GAAAGGCCAT 
GGAATCAGAC TGCTGGAAGC TCAGATAGCC AGCGGTGGGA TTATTGATCC CAAAGGTAGC 
CATCGAATTG ATGTGGAAGT AGCTTACAGG AAGGGTTACT TTGACCGTGA GATGAATGAG 
ATTTTGTCCT ATGAAGGGGA TGACACCAAA GGTTTCTTTG ATCCCAACAC CCATGAAAAC 
CTCACATATC TAGAGTTGAA AAAGAGGTGC ATTAAAGATC CCAAGACTGG GCTCATGCTT 
TTGCCTTTAA ATGACAAGCA GAAACCAAAG CAGACGACAA CCCAGAAGAA CACACTACGC 
AAAAGAAGAG TAGTGATCGT AGACCCTGAC ACTGGTAAAG AGATGACTGT ACGAGAAGCG 
TACCATCGAG AGTTAATCGA CTATGACACC TTTCTGGAAC TTTCCGAACA AGAGTGCGAG 
TGGGAGGAGA TCACGATTGA AACATCTGAT GGAAACAAGC GTTTACTTAT TGTCGATCGC 
AAAACTGGAA TCCAGTATGA CATTCAGGAG TCCCTACAGA GGGGAATTAT CAATAAACAA 
ACTTTGGAAA AGTACCGTGC AGGGACTATG ACGCTCACCG AGTTTGCAGC TCTGATCACT 
AGTAAAAGCA ACAGCTCTGA GCTTGCCATC TTCTCCAGCA GTCCTGAGGA TGTAGCCACC 
TGCAGCAGTC CCACACAGCC ATCATCCCCA ACCGTCCGCA AACGATTTGC AAGTGTTTCC 
ATAACTCTAT CCCCTCCATC TGATATTTTT GACGACCAGA GCCCTGTGGG AGCAATATTC 
GATACGGAGA CCCTTGAGAA GATAACCATC CCCGAGGCTC AGCGACGTGG AATAGTGGAC 
AACATCACCG CTCAGCGTCT TCTTGAAGCG CAGGTTTGCT CCGGAGGAAT TATCAATCCC 
GCTACAGGCC AGAGACTATC TCTGAAAGAC GCCGTACAGC AAAGCCTCAT CGATGAAGAC 
ATGAGCGTCA AGCTAAAACC AGCGCAGAAG GCTTATGACG GTTTTGAGGA TGTGAAGACC 
AAGAGGAAAC TTTCTGCTGC CGAAGCCATG AAGGAAAAAT GGTTGCCCTA CGAAGCCGGC 
CAGAGGTTTC TAGAGTTCCA GTATCTCACA GGTGGACTCA TAGAGCCAGG AACTGGACGC 
CGTGTCAGCA TTGAGGAGGC CATCCGGAAA GGGTGGCTTG ATGGCAAAGG TGCACAAAAG 
CTGCAAGATA CCAGGAACTA CATTAAGAAC CTTACCTGTC CCAAAACCAA GCTTAAAATC 
TCATACAAAG AGGCGATGGA TAACTGCATG GTGGAGGAGA ACAATGGCAT GAAGATGCTC 
CAGGCGACCT CCATGTCTAC CAAAGGCATA AGCAGCCCCT ATAATGTGTC CTCTGGGCCA 
AGCTCTCGCT CTGGATCCAG AGCCGGATCC CGAACAGGCT CAAGAAGTGG ATCGCGCAGA 
GGCAGTGTCG ACTACAGCTC TTCATCTGTT TCTTACACCT TCTTCTCCTC TGCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dspa ( XM_005171104.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171161.1 Danio rerio desmoplakin a (dspa), transcript variant X2, mRNA.

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CloneID	ODa06596
Clone ID Related Accession (Same CDS sequence)	XM_005171104.3 , XM_005171104.4
Accession Version	XM_005171104.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	desmoplakin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171104.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGT CCGGTTCTCA GACCAGGCTC CACCAGATCA GCCGCAGGTC CAGTTCGCGG 
CCAGACCTCA CCGCCGTCGG CTACAGTTTA CCGAGGAACG ACGGAGCTCA GTTTTTCACG 
AGTGGAAACG GATACCAGTC CGACTATAAT GACGGCTACA TGCAGACCTA CACGCAGACT 
TTCTCCAAAA ACTCTCAGGG TGGAGGAGCT GGAGGAGCCC AGAGCATGTC TTCCATGGCG 
GTTCAGAAGC GAGCTCAGAT GTTGTACAGT GACTGCATGG GATTCCTGCA GCGAGCGCAG 
GGCCTCCTGG AGACTATGGG ACCTGCTTCT GAGGTGGACA AGAACATGAT GGCAGCGCAG 
GACGCCATGG ATCAGCTGCG GAAATGTGCG GTGGACATGA GGAACGCCGG TCTGCCTAAT 
GACATGATCC TCCGCAGTTT GGAGGACTGT CATAGCTTTT ATGCAGACAT TCGCAACTCC 
ATCACGGGCA CCACCATCAG GAGGACTGGC GGCACCGTCA GCGGCACCAT CGGTGGCACC 
ATCAGTGGCA CCATTGGTGG CACTGTCGGC GGCTGGGACG ATCCCAGCAA GAGCTTTCAG 
GACGCACTGT CATGGATCAA CCAGAAGAAG AGACTCATTG AAACGTCAGG CTTCGGTGAA 
AATTCAGAAG CCATTAGCCA ACAAATCCTG AATCACAACA AGTTCCACAG TTCAATCCAG 
AGAAGCCAGG AGGTGGACAG AGCCCGCGAC GATCTGATGC AGAGCAGGGA AAAGGCTGGA 
CTACATGCTC TGGACCAAGA GTGGGACAGT TTACAGAAAA TGTCCCATGC ACGTATGGAT 
CATCTGCGAG ACCTACAGAG CATCATCGAG GAGATTTCCA GAGCCATCAT GTGGGTCAAC 
GACAGAGAGG AGGAGGAGCT GGTGTTTGAC TGGGGCGATA AAAACATTGA CAACTACATC 
CCTCAAAAAC AAGAGAGCTA CTCGAAACTG ATGAGTGATC TGGAGGAGAA GGAGAAAGAG 
CTGAATAAGC TAAAGGCAAA GGTGGACATG CTGCTGAAGA ACCAGCACCC GGCATCTGAT 
AAGATAGAGG CTTACATGGA CACACTGCAA ACCCAGTGGA GCTGGTTACT GCAGATCACC 
AAGTGCATCC ATGTTCACCT GAAGGAGAAC GCAGCCTACA GCCAGTTCTT CAAGGAGGCC 
AATGAGACGT ACAGCAGACT GCAGAAAGAC CATGAAAACA TCCGGAAGAA GTTCGGCTGC 
GACAAAACCA CACCTCTGGA GAACCTCAAC GACATGCTGA AAAACCTGGA GAAAGAGAAG 
GAGAAGGTCA TCGAGAATAA GCGTCAGGTT CAGACGCTGG TCAGCAAGTC CAAGAACATC 
GTGTGTCTGA AACCTCGTAA TCCAGAGGAG AAGAGCAGCA GTCCGGTCAT AGTGCAAGCT 
CTGTGTGACT TCAAACAGGA TCAAAAAGGC ATTATGAAGG GCAATGAAGG TATCCTGAAA 
GACAACTCTC AGCGCAGTAA GTGGCACGTG ACTGGACCTG GAGGACTGGA AATGCTCATC 
CCGTCTGTGT GTCTGATCAT CCCACCGCCA AACCCTCTGA GCATCAGCCT GGCAAACAAG 
AACGAGCAGT ATTATGAAGC CATTATGTCC ATATGGAGTC AGCTGTACAT CAACATCAAG 
AGTCTGATAT CCTGGCAGTA CTGTCTGAGA GACATCCAGC ACATCAACTC CCTCACTCTC 
TCTATGCTGA CGCAAATGCG TCCTGAGGAG TATCGCCAAA CCATCAAGAA CCTGGAGATC 
CACTACCAGG AGTTTCTGCG CAACAGTCTT GGCTCTGAGA TGTTCGGTGA TGACGACAAG 
AGAAAGATGG AGCTTCAGTA TGCCGGAGCT CAGTCCCACT ACGACCAGCT GGTGATCCAA 
CTGCCCAACT ACAGGGAGAA TGGAGTGATC AGAGAGGTGG TGATGGTGGA GACAGATTCC 
AAGCTGAAAT CTGAGGTGTC GAGTGGGAAG ACTGCGACCG GGGTGTCGAG TGGGAAGACT 
GCGACAGGAG TGTCGAGTGG GAAGACTTCG TCTTCTGTGT CGGTTTCGGG TCTTAATGTG 
AGTCTGCTGT CGGACCTCAG CGCTCTGAGA CGGAGACTAG AGACAGCAGA ATCCGGGCTC 
ACCAAACATC TGCACGTTCC GCTGAAGGAG AACAGCGTTC AGGAGTGCTC ACAGAGACTG 
GCGCAGCTAC AGGCTGTTCA TCGTGATCTG GACTCCATCA GGGACGAGTA TCTGCATCTG 
AGGGAGAAGA TTATGAGAGA GCTGGAGGGC AGCAGTGACC CTGAGCAGAG CCGATACCTG 
CGCGCAGAAC TGGACCTGAT CAACCAGAAA CTGGGCAGCC TGCAGGGCTT CTCCAGCGCA 
TACATCCAGA GGCTCGGTGC TCTACAAGCT CTGCTCCAGC ACCTCCTGCA GGCCGAGGAC 
ATCATTAAAG TCCACGAGGC TCGTCTGACC GAGAAAGAAA CCTCTTCTCT TGACCTGAAC 
GAAGTGGAAA AGTACTGCAT GACTCTCAAG ACTATGAAAG CAGAGCTGGA GCAGAAGAAG 
GGTGTTTTGA AGGCGATGGA GACGGAGCTG TCTAAAGCTG TGCACTGGAA CAGTCAGATT 
GACCAGTCTT TCCATCAGTG TGATGTTGAC CTGTCCAGAT ACACTGAGCT GGTCGGACAG 
ATGACTGACC GCTGGCGCAG GATTGTGACT CAGATTGATA GCAGGACATG GGACCTGGAG 
AAGCAAGAGA AACAGCTGAA TCACTACCAG CAGACCAGCT CCACGATAAA CAGATGGATT 
CAGGACACAC GTCAGCGCCA AGACACCCTG CAGATTACCA AATTCAACAG CGTGGACAAT 
TTAATGGACC ACCTCAACCA GCAAAAAGCG CTTTATTCTG AGATCAAGGG GAAGAAAGAA 
AAGGTGGATG CTGTGGTCAA AGACTCAGAC ACATGTGCTG CCTCCATAAA GGACTACGAG 
CTCCAGTTGG CGTCTTATAG TGCAGGTCTG GAGACTCTGC TCAACATTCC CATCAAAAAG 
ACTATGCTGC AGTCTCCAGC CACTGTTCTC CGGGAGGAGG CAACAGATTT GCAGTCTCGC 
TACATTGAGC TCCTGACACG ATCAAGTGAC TACTACAAAT TCCTTGGGGA AATGCTGAAA 
AACATGGAGG AGTTAAAGAT GCGGAACACC AAAATTGAGC TTTTGGAGGA GGAACTGAGA 
CGCCTCAAGG ACAACCTCAA AGATCAAAAC CAGAAGAACA AATCACTGGA GGACAGTTTG 
ACTCGCTTCC GCCTGGAGCT GACCCAGTCC AAAGAGCAGC TCATATCTAT GGAGGAGGTG 
AAGAGAACCC AGGCCAGGCA GTGCAACACT GCTCAGGAAA GCCTGGATAG TACTCAGAAC 
CAGCTGAAGA GTTTACAAGA TGAGATGTCC CGCCTTACAT TCCTCATCGA GGAGGAAAAG 
CGCAAGCGAA GGCTGGCCGA GGAGCGTTAC ACCAACCAGC AGGAGGAGTA TGAGCTGGCC 
ATGCGCAAGA GACAGAAAGA ACTGGAGGAG CTCACTTTGT CCAAAAGTCA ATTTGAAAGA 
GCCATCAAAG AGAAAGAGCG GGAGATTGAA CGGCTGAAAT TACAGCTGCA GGATGAGGCC 
TCCCGACGCT CGGCAGCAGA ACTGGAGACC TCAAAGACAT CCATGATGAT CCAGCGATCT 
GATTCAAACT ACAAAGACAT TGTGCAAGAG AGAGATAGTC TCTTAATCAA ACTGAAGCTG 
CTGCAACAGG ACAAAGATAA GCAGCAGCGA TATGAAGAGG AGCTCAGGCG CATCAAACTT 
ACCTTGGAAT CAGAGACCAA ACAGAAGCAA CGTCTCCAGG ATGAAATCGA TAAAATCACC 
AAGGATTTCA AGTACTGGAA AAGCCAGTAC GAGTTAAAAG AGGGGCAGAT CAGACAGAGC 
GAGATGGATA GAGACAGAGT AGAGAGAGAC CGAGCCTCCT TACAGAGTGA GATCCAAAGA 
TTGACCGCTG AGCTGAGAAG TGTTGAGGAA CGCTACAGAG GTCGTCTTCA GAGCTCAGAC 
AAGGAAATCT CGGAGCTAAT GCGTAAGAAG GAGTCTTTGG AAATTGAGCT GAGGAGATTG 
CAACAGCGTC CAGCTGCAAC CTGGAAGCAA ACCCAGACAG ATGAGATCTC TAAGCCAGTT 
GTTGAGCAGA AATTGACAGT GCAAGGTCTT CGTGGTGAGG TCTCACTTAC AGAGCTTGTC 
GAGTCCGACT TGCTCGATCA AACTGATTTG GACAAAATAA ACCGAGGACA GCTAACCAGT 
AAAGATATCG AGCACAAGCT CAAATCATAC CTTGGTGGCT CTGATTGTAT CGCTGGCATC 
TATGATGAGG CCAAGGATAG AGTCATGCCT TTTTACCAGG CGATGAAAGA TGGTCTACTC 
CGACGAGGAA CCACACTGGA GCTCCTGGAG GCCCAGGCTG CCTCTGGTTT CATAATTGAC 
CCAGTTAACA ATGTCTGTAT GACCGTTGAA GAAGCTTGGA AGAGAGGTCT TGTAGGAAAG 
GAGTTCAAAG ACAAACTCCT GTCTGCTGAG AAGGCTGTGA CGGGATACAA AGATCCTGCA 
ACAGGAAAGA TCATCTCACT CTTCCAAGCT ATTGAGAAAG AGATCATTGA GAAAGGCCAT 
GGAATCAGAC TGCTGGAAGC TCAGATAGCC AGCGGTGGGA TTATTGATCC CAAAGGTAGC 
CATCGAATTG ATGTGGAAGT AGCTTACAGG AAGGGTTACT TTGACCGTGA GATGAATGAG 
ATTTTGTCCT ATGAAGGGGA TGACACCAAA GGTTTCTTTG ATCCCAACAC CCATGAAAAC 
CTCACATATC TAGAGTTGAA AAAGAGGTGC ATTAAAGATC CCAAGACTGG GCTCATGCTT 
TTGCCTTTAA ATGACAAGCA GAAACCAAAG CAGACGACAA CCCAGAAGAA CACACTACGC 
AAAAGAAGAG TAGTGATCGT AGACCCTGAC ACTGGTAAAG AGATGACTGT ACGAGAAGCG 
TACCATCGAG AGTTAATCGA CTATGACACC TTTCTGGAAC TTTCCGAACA AGAGTGCGAG 
TGGGAGGAGA TCACGATTGA AACATCTGAT GGAAACAAGC GTTTACTTAT TGTCGATCGC 
AAAACTGGAA TCCAGTATGA CATTCAGGAG TCCCTACAGA GGGGAATTAT CAATAAACAA 
ACTTTGGAAA AGTACCGTGC AGGGACTATG ACGCTCACCG AGTTTGCAGC TCTGATCACT 
AGTAAAAGCA ACAGCTCTGA GCTTGCCATC TTCTCCAGCA GTCCTGAGGA TGTAGCCACC 
TGCAGCAGTC CCACACAGCC ATCATCCCCA ACCGTCCGCA AACGATTTGC AAGTGTTTCC 
ATAACTCTAT CCCCTCCATC TGATATTTTT GACGACCAGA GCCCTGTGGG AGCAATATTC 
GATACGGAGA CCCTTGAGAA GATAACCATC CCCGAGGCTC AGCGACGTGG AATAGTGGAC 
AACATCACCG CTCAGCGTCT TCTTGAAGCG CAGGTTTGCT CCGGAGGAAT TATCAATCCC 
GCTACAGGCC AGAGACTATC TCTGAAAGAC GCCGTACAGC AAAGCCTCAT CGATGAAGAC 
ATGAGCGTCA AGCTAAAACC AGCGCAGAAG GCTTATGACG GTTTTGAGGA TGTGAAGACC 
AAGAGGAAAC TTTCTGCTGC CGAAGCCATG AAGGAAAAAT GGTTGCCCTA CGAAGCCGGC 
CAGAGGTTTC TAGAGTTCCA GTATCTCACA GGTGGACTCA TAGAGCCAGG AACTGGACGC 
CGTGTCAGCA TTGAGGAGGC CATCCGGAAA GGGTGGCTTG ATGGCAAAGG TGCACAAAAG 
CTGCAAGATA CCAGGAACTA CATTAAGAAC CTTACCTGTC CCAAAACCAA GCTTAAAATC 
TCATACAAAG AGGCGATGGA TAACTGCATG GTGGAGGAGA ACAATGGCAT GAAGATGCTC 
CAGGCGACCT CCATGTCTAC CAAAGGCATA AGCAGCCCCT ATAATGTGTC CTCTGGGCCA 
AGCTCTCGCT CTGGATCCAG AGCCGGATCC CGAACAGGCT CAAGAAGTGG ATCGCGCAGA 
GGCAGTGTCG ACTACAGCTC TTCATCTGTT TCTTACACCT TCTTCTCCTC TGCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171161.1
CDS	115..6294
Translation	MSLSGSQTRLHQISRRSSSRPDLTAVGYSLPRNDGAQFFTSGNGYQSDYNDGYMQTYTQTFSKNSQGGGAGGAQSMSSMAVQKRAQMLYSDCMGFLQRAQGLLETMGPASEVDKNMMAAQDAMDQLRKCAVDMRNAGLPNDMILRSLEDCHSFYADIRNSITGTTIRRTGGTVSGTIGGTISGTIGGTVGGWDDPSKSFQDALSWINQKKRLIETSGFGENSEAISQQILNHNKFHSSIQRSQEVDRARDDLMQSREKAGLHALDQEWDSLQKMSHARMDHLRDLQSIIEEISRAIMWVNDREEEELVFDWGDKNIDNYIPQKQESYSKLMSDLEEKEKELNKLKAKVDMLLKNQHPASDKIEAYMDTLQTQWSWLLQITKCIHVHLKENAAYSQFFKEANETYSRLQKDHENIRKKFGCDKTTPLENLNDMLKNLEKEKEKVIENKRQVQTLVSKSKNIVCLKPRNPEEKSSSPVIVQALCDFKQDQKGIMKGNEGILKDNSQRSKWHVTGPGGLEMLIPSVCLIIPPPNPLSISLANKNEQYYEAIMSIWSQLYINIKSLISWQYCLRDIQHINSLTLSMLTQMRPEEYRQTIKNLEIHYQEFLRNSLGSEMFGDDDKRKMELQYAGAQSHYDQLVIQLPNYRENGVIREVVMVETDSKLKSEVSSGKTATGVSSGKTATGVSSGKTSSSVSVSGLNVSLLSDLSALRRRLETAESGLTKHLHVPLKENSVQECSQRLAQLQAVHRDLDSIRDEYLHLREKIMRELEGSSDPEQSRYLRAELDLINQKLGSLQGFSSAYIQRLGALQALLQHLLQAEDIIKVHEARLTEKETSSLDLNEVEKYCMTLKTMKAELEQKKGVLKAMETELSKAVHWNSQIDQSFHQCDVDLSRYTELVGQMTDRWRRIVTQIDSRTWDLEKQEKQLNHYQQTSSTINRWIQDTRQRQDTLQITKFNSVDNLMDHLNQQKALYSEIKGKKEKVDAVVKDSDTCAASIKDYELQLASYSAGLETLLNIPIKKTMLQSPATVLREEATDLQSRYIELLTRSSDYYKFLGEMLKNMEELKMRNTKIELLEEELRRLKDNLKDQNQKNKSLEDSLTRFRLELTQSKEQLISMEEVKRTQARQCNTAQESLDSTQNQLKSLQDEMSRLTFLIEEEKRKRRLAEERYTNQQEEYELAMRKRQKELEELTLSKSQFERAIKEKEREIERLKLQLQDEASRRSAAELETSKTSMMIQRSDSNYKDIVQERDSLLIKLKLLQQDKDKQQRYEEELRRIKLTLESETKQKQRLQDEIDKITKDFKYWKSQYELKEGQIRQSEMDRDRVERDRASLQSEIQRLTAELRSVEERYRGRLQSSDKEISELMRKKESLEIELRRLQQRPAATWKQTQTDEISKPVVEQKLTVQGLRGEVSLTELVESDLLDQTDLDKINRGQLTSKDIEHKLKSYLGGSDCIAGIYDEAKDRVMPFYQAMKDGLLRRGTTLELLEAQAASGFIIDPVNNVCMTVEEAWKRGLVGKEFKDKLLSAEKAVTGYKDPATGKIISLFQAIEKEIIEKGHGIRLLEAQIASGGIIDPKGSHRIDVEVAYRKGYFDREMNEILSYEGDDTKGFFDPNTHENLTYLELKKRCIKDPKTGLMLLPLNDKQKPKQTTTQKNTLRKRRVVIVDPDTGKEMTVREAYHRELIDYDTFLELSEQECEWEEITIETSDGNKRLLIVDRKTGIQYDIQESLQRGIINKQTLEKYRAGTMTLTEFAALITSKSNSSELAIFSSSPEDVATCSSPTQPSSPTVRKRFASVSITLSPPSDIFDDQSPVGAIFDTETLEKITIPEAQRRGIVDNITAQRLLEAQVCSGGIINPATGQRLSLKDAVQQSLIDEDMSVKLKPAQKAYDGFEDVKTKRKLSAAEAMKEKWLPYEAGQRFLEFQYLTGGLIEPGTGRRVSIEEAIRKGWLDGKGAQKLQDTRNYIKNLTCPKTKLKISYKEAMDNCMVEENNGMKMLQATSMSTKGISSPYNVSSGPSSRSGSRAGSRTGSRSGSRRGSVDYSSSSVSYTFFSSAS

Target ORF information:

RefSeq Version	XM_005171104.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio desmoplakin a (dspa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171104.4

ORF Insert Sequence:

ATGAGTTTGT CCGGTTCTCA GACCAGGCTC CACCAGATCA GCCGCAGGTC CAGTTCGCGG 
CCAGACCTCA CCGCCGTCGG CTACAGTTTA CCGAGGAACG ACGGAGCTCA GTTTTTCACG 
AGTGGAAACG GATACCAGTC CGACTATAAT GACGGCTACA TGCAGACCTA CACGCAGACT 
TTCTCCAAAA ACTCTCAGGG TGGAGGAGCT GGAGGAGCCC AGAGCATGTC TTCCATGGCG 
GTTCAGAAGC GAGCTCAGAT GTTGTACAGT GACTGCATGG GATTCCTGCA GCGAGCGCAG 
GGCCTCCTGG AGACTATGGG ACCTGCTTCT GAGGTGGACA AGAACATGAT GGCAGCGCAG 
GACGCCATGG ATCAGCTGCG GAAATGTGCG GTGGACATGA GGAACGCCGG TCTGCCTAAT 
GACATGATCC TCCGCAGTTT GGAGGACTGT CATAGCTTTT ATGCAGACAT TCGCAACTCC 
ATCACGGGCA CCACCATCAG GAGGACTGGC GGCACCGTCA GCGGCACCAT CGGTGGCACC 
ATCAGTGGCA CCATTGGTGG CACTGTCGGC GGCTGGGACG ATCCCAGCAA GAGCTTTCAG 
GACGCACTGT CATGGATCAA CCAGAAGAAG AGACTCATTG AAACGTCAGG CTTCGGTGAA 
AATTCAGAAG CCATTAGCCA ACAAATCCTG AATCACAACA AGTTCCACAG TTCAATCCAG 
AGAAGCCAGG AGGTGGACAG AGCCCGCGAC GATCTGATGC AGAGCAGGGA AAAGGCTGGA 
CTACATGCTC TGGACCAAGA GTGGGACAGT TTACAGAAAA TGTCCCATGC ACGTATGGAT 
CATCTGCGAG ACCTACAGAG CATCATCGAG GAGATTTCCA GAGCCATCAT GTGGGTCAAC 
GACAGAGAGG AGGAGGAGCT GGTGTTTGAC TGGGGCGATA AAAACATTGA CAACTACATC 
CCTCAAAAAC AAGAGAGCTA CTCGAAACTG ATGAGTGATC TGGAGGAGAA GGAGAAAGAG 
CTGAATAAGC TAAAGGCAAA GGTGGACATG CTGCTGAAGA ACCAGCACCC GGCATCTGAT 
AAGATAGAGG CTTACATGGA CACACTGCAA ACCCAGTGGA GCTGGTTACT GCAGATCACC 
AAGTGCATCC ATGTTCACCT GAAGGAGAAC GCAGCCTACA GCCAGTTCTT CAAGGAGGCC 
AATGAGACGT ACAGCAGACT GCAGAAAGAC CATGAAAACA TCCGGAAGAA GTTCGGCTGC 
GACAAAACCA CACCTCTGGA GAACCTCAAC GACATGCTGA AAAACCTGGA GAAAGAGAAG 
GAGAAGGTCA TCGAGAATAA GCGTCAGGTT CAGACGCTGG TCAGCAAGTC CAAGAACATC 
GTGTGTCTGA AACCTCGTAA TCCAGAGGAG AAGAGCAGCA GTCCGGTCAT AGTGCAAGCT 
CTGTGTGACT TCAAACAGGA TCAAAAAGGC ATTATGAAGG GCAATGAAGG TATCCTGAAA 
GACAACTCTC AGCGCAGTAA GTGGCACGTG ACTGGACCTG GAGGACTGGA AATGCTCATC 
CCGTCTGTGT GTCTGATCAT CCCACCGCCA AACCCTCTGA GCATCAGCCT GGCAAACAAG 
AACGAGCAGT ATTATGAAGC CATTATGTCC ATATGGAGTC AGCTGTACAT CAACATCAAG 
AGTCTGATAT CCTGGCAGTA CTGTCTGAGA GACATCCAGC ACATCAACTC CCTCACTCTC 
TCTATGCTGA CGCAAATGCG TCCTGAGGAG TATCGCCAAA CCATCAAGAA CCTGGAGATC 
CACTACCAGG AGTTTCTGCG CAACAGTCTT GGCTCTGAGA TGTTCGGTGA TGACGACAAG 
AGAAAGATGG AGCTTCAGTA TGCCGGAGCT CAGTCCCACT ACGACCAGCT GGTGATCCAA 
CTGCCCAACT ACAGGGAGAA TGGAGTGATC AGAGAGGTGG TGATGGTGGA GACAGATTCC 
AAGCTGAAAT CTGAGGTGTC GAGTGGGAAG ACTGCGACCG GGGTGTCGAG TGGGAAGACT 
GCGACAGGAG TGTCGAGTGG GAAGACTTCG TCTTCTGTGT CGGTTTCGGG TCTTAATGTG 
AGTCTGCTGT CGGACCTCAG CGCTCTGAGA CGGAGACTAG AGACAGCAGA ATCCGGGCTC 
ACCAAACATC TGCACGTTCC GCTGAAGGAG AACAGCGTTC AGGAGTGCTC ACAGAGACTG 
GCGCAGCTAC AGGCTGTTCA TCGTGATCTG GACTCCATCA GGGACGAGTA TCTGCATCTG 
AGGGAGAAGA TTATGAGAGA GCTGGAGGGC AGCAGTGACC CTGAGCAGAG CCGATACCTG 
CGCGCAGAAC TGGACCTGAT CAACCAGAAA CTGGGCAGCC TGCAGGGCTT CTCCAGCGCA 
TACATCCAGA GGCTCGGTGC TCTACAAGCT CTGCTCCAGC ACCTCCTGCA GGCCGAGGAC 
ATCATTAAAG TCCACGAGGC TCGTCTGACC GAGAAAGAAA CCTCTTCTCT TGACCTGAAC 
GAAGTGGAAA AGTACTGCAT GACTCTCAAG ACTATGAAAG CAGAGCTGGA GCAGAAGAAG 
GGTGTTTTGA AGGCGATGGA GACGGAGCTG TCTAAAGCTG TGCACTGGAA CAGTCAGATT 
GACCAGTCTT TCCATCAGTG TGATGTTGAC CTGTCCAGAT ACACTGAGCT GGTCGGACAG 
ATGACTGACC GCTGGCGCAG GATTGTGACT CAGATTGATA GCAGGACATG GGACCTGGAG 
AAGCAAGAGA AACAGCTGAA TCACTACCAG CAGACCAGCT CCACGATAAA CAGATGGATT 
CAGGACACAC GTCAGCGCCA AGACACCCTG CAGATTACCA AATTCAACAG CGTGGACAAT 
TTAATGGACC ACCTCAACCA GCAAAAAGCG CTTTATTCTG AGATCAAGGG GAAGAAAGAA 
AAGGTGGATG CTGTGGTCAA AGACTCAGAC ACATGTGCTG CCTCCATAAA GGACTACGAG 
CTCCAGTTGG CGTCTTATAG TGCAGGTCTG GAGACTCTGC TCAACATTCC CATCAAAAAG 
ACTATGCTGC AGTCTCCAGC CACTGTTCTC CGGGAGGAGG CAACAGATTT GCAGTCTCGC 
TACATTGAGC TCCTGACACG ATCAAGTGAC TACTACAAAT TCCTTGGGGA AATGCTGAAA 
AACATGGAGG AGTTAAAGAT GCGGAACACC AAAATTGAGC TTTTGGAGGA GGAACTGAGA 
CGCCTCAAGG ACAACCTCAA AGATCAAAAC CAGAAGAACA AATCACTGGA GGACAGTTTG 
ACTCGCTTCC GCCTGGAGCT GACCCAGTCC AAAGAGCAGC TCATATCTAT GGAGGAGGTG 
AAGAGAACCC AGGCCAGGCA GTGCAACACT GCTCAGGAAA GCCTGGATAG TACTCAGAAC 
CAGCTGAAGA GTTTACAAGA TGAGATGTCC CGCCTTACAT TCCTCATCGA GGAGGAAAAG 
CGCAAGCGAA GGCTGGCCGA GGAGCGTTAC ACCAACCAGC AGGAGGAGTA TGAGCTGGCC 
ATGCGCAAGA GACAGAAAGA ACTGGAGGAG CTCACTTTGT CCAAAAGTCA ATTTGAAAGA 
GCCATCAAAG AGAAAGAGCG GGAGATTGAA CGGCTGAAAT TACAGCTGCA GGATGAGGCC 
TCCCGACGCT CGGCAGCAGA ACTGGAGACC TCAAAGACAT CCATGATGAT CCAGCGATCT 
GATTCAAACT ACAAAGACAT TGTGCAAGAG AGAGATAGTC TCTTAATCAA ACTGAAGCTG 
CTGCAACAGG ACAAAGATAA GCAGCAGCGA TATGAAGAGG AGCTCAGGCG CATCAAACTT 
ACCTTGGAAT CAGAGACCAA ACAGAAGCAA CGTCTCCAGG ATGAAATCGA TAAAATCACC 
AAGGATTTCA AGTACTGGAA AAGCCAGTAC GAGTTAAAAG AGGGGCAGAT CAGACAGAGC 
GAGATGGATA GAGACAGAGT AGAGAGAGAC CGAGCCTCCT TACAGAGTGA GATCCAAAGA 
TTGACCGCTG AGCTGAGAAG TGTTGAGGAA CGCTACAGAG GTCGTCTTCA GAGCTCAGAC 
AAGGAAATCT CGGAGCTAAT GCGTAAGAAG GAGTCTTTGG AAATTGAGCT GAGGAGATTG 
CAACAGCGTC CAGCTGCAAC CTGGAAGCAA ACCCAGACAG ATGAGATCTC TAAGCCAGTT 
GTTGAGCAGA AATTGACAGT GCAAGGTCTT CGTGGTGAGG TCTCACTTAC AGAGCTTGTC 
GAGTCCGACT TGCTCGATCA AACTGATTTG GACAAAATAA ACCGAGGACA GCTAACCAGT 
AAAGATATCG AGCACAAGCT CAAATCATAC CTTGGTGGCT CTGATTGTAT CGCTGGCATC 
TATGATGAGG CCAAGGATAG AGTCATGCCT TTTTACCAGG CGATGAAAGA TGGTCTACTC 
CGACGAGGAA CCACACTGGA GCTCCTGGAG GCCCAGGCTG CCTCTGGTTT CATAATTGAC 
CCAGTTAACA ATGTCTGTAT GACCGTTGAA GAAGCTTGGA AGAGAGGTCT TGTAGGAAAG 
GAGTTCAAAG ACAAACTCCT GTCTGCTGAG AAGGCTGTGA CGGGATACAA AGATCCTGCA 
ACAGGAAAGA TCATCTCACT CTTCCAAGCT ATTGAGAAAG AGATCATTGA GAAAGGCCAT 
GGAATCAGAC TGCTGGAAGC TCAGATAGCC AGCGGTGGGA TTATTGATCC CAAAGGTAGC 
CATCGAATTG ATGTGGAAGT AGCTTACAGG AAGGGTTACT TTGACCGTGA GATGAATGAG 
ATTTTGTCCT ATGAAGGGGA TGACACCAAA GGTTTCTTTG ATCCCAACAC CCATGAAAAC 
CTCACATATC TAGAGTTGAA AAAGAGGTGC ATTAAAGATC CCAAGACTGG GCTCATGCTT 
TTGCCTTTAA ATGACAAGCA GAAACCAAAG CAGACGACAA CCCAGAAGAA CACACTACGC 
AAAAGAAGAG TAGTGATCGT AGACCCTGAC ACTGGTAAAG AGATGACTGT ACGAGAAGCG 
TACCATCGAG AGTTAATCGA CTATGACACC TTTCTGGAAC TTTCCGAACA AGAGTGCGAG 
TGGGAGGAGA TCACGATTGA AACATCTGAT GGAAACAAGC GTTTACTTAT TGTCGATCGC 
AAAACTGGAA TCCAGTATGA CATTCAGGAG TCCCTACAGA GGGGAATTAT CAATAAACAA 
ACTTTGGAAA AGTACCGTGC AGGGACTATG ACGCTCACCG AGTTTGCAGC TCTGATCACT 
AGTAAAAGCA ACAGCTCTGA GCTTGCCATC TTCTCCAGCA GTCCTGAGGA TGTAGCCACC 
TGCAGCAGTC CCACACAGCC ATCATCCCCA ACCGTCCGCA AACGATTTGC AAGTGTTTCC 
ATAACTCTAT CCCCTCCATC TGATATTTTT GACGACCAGA GCCCTGTGGG AGCAATATTC 
GATACGGAGA CCCTTGAGAA GATAACCATC CCCGAGGCTC AGCGACGTGG AATAGTGGAC 
AACATCACCG CTCAGCGTCT TCTTGAAGCG CAGGTTTGCT CCGGAGGAAT TATCAATCCC 
GCTACAGGCC AGAGACTATC TCTGAAAGAC GCCGTACAGC AAAGCCTCAT CGATGAAGAC 
ATGAGCGTCA AGCTAAAACC AGCGCAGAAG GCTTATGACG GTTTTGAGGA TGTGAAGACC 
AAGAGGAAAC TTTCTGCTGC CGAAGCCATG AAGGAAAAAT GGTTGCCCTA CGAAGCCGGC 
CAGAGGTTTC TAGAGTTCCA GTATCTCACA GGTGGACTCA TAGAGCCAGG AACTGGACGC 
CGTGTCAGCA TTGAGGAGGC CATCCGGAAA GGGTGGCTTG ATGGCAAAGG TGCACAAAAG 
CTGCAAGATA CCAGGAACTA CATTAAGAAC CTTACCTGTC CCAAAACCAA GCTTAAAATC 
TCATACAAAG AGGCGATGGA TAACTGCATG GTGGAGGAGA ACAATGGCAT GAAGATGCTC 
CAGGCGACCT CCATGTCTAC CAAAGGCATA AGCAGCCCCT ATAATGTGTC CTCTGGGCCA 
AGCTCTCGCT CTGGATCCAG AGCCGGATCC CGAACAGGCT CAAGAAGTGG ATCGCGCAGA 
GGCAGTGTCG ACTACAGCTC TTCATCTGTT TCTTACACCT TCTTCTCCTC TGCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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dspa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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