med24 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	med24
Entrez Gene ID	323621
Full Name	mediator complex subunit 24
Synonyms	fc05c04,thrap4,trap100,wu:fc05c04
General protein information	Preferred Names mediator complex subunit 24 Names mediator of RNA polymerase II transcription subunit 24 100 kDa thyroid hormone receptor associated protein crsp100 crsp4 lessen lsn protein lessen thyroid hormone receptor associated protein 4 thyroid hormone receptor-associated protein 4 homolog trap100 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039062.1	NP_001034151.1	mediator of RNA polymerase II transcription subunit 24
XM_009306486.2	XP_009304761.1	mediator of RNA polymerase II transcription subunit 24 isoform X1
XM_009306486.3	XP_009304761.1	mediator of RNA polymerase II transcription subunit 24 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MED24	XP_005220831.1
Anopheles gambiae (African malaria mosquito)	MED24_ANOGA	XP_315936.4
Macaca mulatta (Rhesus monkey)	MED24	XP_001087996.2
Mus musculus (house mouse)	Med24	NP_035999.2
Homo sapiens (human)	MED24	NP_055630.2
Pan troglodytes (chimpanzee)	MED24	XP_001171125.2
Canis lupus familiaris (dog)	MED24	XP_863448.2
Danio rerio (zebrafish)	med24	NP_001034151.1
Drosophila melanogaster (fruit fly)	MED24	NP_648185.1
Rattus norvegicus (Norway rat)	Med24	NP_001029251.1
Gallus gallus (chicken)	MED24	NP_001026534.1
Xenopus tropicalis (tropical clawed frog)	LOC100491557	XP_004911860.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

The zebrafish mutant lessen: an experimental model for congenital enteric neuropathies.
Uyttebroek L, Shepherd IT, Vanden Berghe P, Hubens G, Timmermans JP, Van Nassauw L
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society28(3)345-57(2016 Mar)

In vivo visualization of the development of the enteric nervous system using a Tg(-8.3bphox2b:Kaede) transgenic zebrafish.
Harrison C, Wabbersen T, Shepherd IT
Genesis (New York, N.Y. : 2000)52(12)985-90(2014 Dec)

Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration.
Liongue C, Hall CJ, O'Connell BA, Crosier P, Ward AC
Blood113(11)2535-46(2009 Mar)

Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development.
Dürr K, Holzschuh J, Filippi A, Ettl AK, Ryu S, Shepherd IT, Driever W
Genetics174(2)693-705(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following med24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38571	NM_001039062.1 Latest version!	Danio rerio mediator complex subunit 24 (med24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa39777	XM_009306486.3 Latest version!	Danio rerio mediator complex subunit 24 (med24), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa39777	XM_009306486.2	Danio rerio mediator complex subunit 24 (med24), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med24 ( NM_001039062.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001034151.1 Danio rerio mediator complex subunit 24 (med24), mRNA.

Price & Availability↑

CloneID	ODa38571
Clone ID Related Accession (Same CDS sequence)	NM_001039062.1
Accession Version	NM_001039062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529942400000
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 24
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ300265.1. ##Evidence-Data-START## Transcript exon combination :: DQ300265.1, BC068408.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGTGG TGAATCTGAA ACAGGCCATC CTACAGGCGT GGAAGGAACG TTGGAGTGAT 
TATCAGTGGG CCATTAACAT TAAGAAGAAC TGTCCCAAAG GAGCTACATG GGATTATCTC 
AATCTAGCAG AAGCTCTTCT GGAGCAGGCT ATGATTGGCC CATCTCCCAA CCCCCTGATC 
CTGTCCTATC TGAAGTATGC CATCAGTTCA CAGATGGTGT CGTATTCTAG TGTGTTAACG 
GCAATCAGCA AGTTTGATGA TTTTTCCCGG GAGCTATGTG TCAAATCATT GCTTGAGATT 
ATGGACATGT TCTCCAACCG ACTAAGCTGT CATGGAAAGG CAGAGGAGTG TATTGGGCTC 
TGCCGCGCCA TGCTGTGTAC CGTGGTTTGG CTCCTGCAGG GCTGTGCATG GTACTGTGAG 
AGACTGCGGG ACCCCAATGA TTTTTCTGCA TTAGAAAGCA GTCTGAGGGC CTGCCTGGAG 
CGCATGGCCA ATCTCTTGCA CAGCACAAAG AACAGAGCCC TGGTACACAT CGCACGCCTG 
GAGGAGCAAG CATCCTGGAC CAATGTAGAG CAAGCTGTGC TCAAGCTGAC AGAAAATCTC 
AGTGCTGTGA CCAATCCAAC TCTGAAGGAA AGTCTTGAGG AGTGTGTTTC ATTGGTCAAA 
AGTATTCCTC AGATGTTGTG TCTTCAGTGT GATCCACCAG TCCACACCAC ATTTCCTTCA 
GTCCATGCCT TCATCATGCT GGAGGGAACC ATGAACCTCA CTGGAGAGAC GCAGCCATTA 
GTGGAGCAGC TAATGATGAT CAAGAGGATG CAGCGGATTC CAGCCCCACT GTTTGTGCTG 
GGGATCTGGA AGGCCTGTTT CACTGGACTT ATCGAGTCTC CAGAGGGAAA CGAGGAGCTC 
AAATGGACGG CCTTCACATT CCTAAAGATT CCTCAGGTTT TACTAAGACT AAAAAAGTAC 
CCACAAGGAG AGAAGACGCA GGATTTTACG GAGGATGTGA ATGTGGCTTT TGAATATCTT 
CTGAATCTCA CTCCTCTGCT GGATAAAGCC GATCAGAGAT GCAACTGTGA CTGCTTGAGT 
CTTTTACTGC AGGAGTGTAA TAAACTGGGG CTGCTCTCTG AAGCAAACAC CGCCACTCTT 
ACTGCAAAAC GGAGTGAGGA CCGAGAACAT GCACCTAGAC TGAAGACAGC AGAAAATGCC 
AACATTCAGC CCAATCCAGG CCTCATCCTG AGAGCCGAAC CAACCGTCAC CAACATCCTT 
AAGACCGTGG ATGCGGATCA TTCTAAGTCT CCCGAGGGAT TGCTGGGAGT TTTGGGTCAC 
ATGCTCTCAG GAAAGAGTCT TGATTTGCTA CTGGCCGCTG CTGCAGCCAC TGGAAAACTC 
AAGTCTTTCG CCCGCAAATT CATCAAGCTG AACGAGTTTC CAAAGCACAT TAGTGGAGAA 
GGATCGAAGT CTGCATCAGT ACGAGCTCTG CTATTCGACA TTTCCTTCCT CATGCTGTGT 
CATGTCGTAC AGACGTATGG ATCAGAGGTG ATTCTTTCAG ACCCCAGCCC ATCAAGCGAG 
ACCCCTTTCT TTGAGACATG GCTTCAGACG TGCATGCCAG AGGAAGGGAA GACCCTGAAT 
CCAGACCACC CTTGCTTTAG ACCTGAGTCT GGGAAGGTGG AGAGCCTGGT GGCCCTGCTT 
AATTCTTCAT CTGAGATGAA GCTTGTTCAG ATGAAATGGC ATGAAATATG TCTTAGTACT 
CCTGCAGCTA TACTTGAGGT CCTGAATGCC TGGGAAAATG GAGTCTTGTC AGTGGAAGCT 
GTTCAGAAGA TCACAGATAA TATAAAAGGA AAGGTGTGCA GTATGGCCAT CTGTGCAGTG 
GCATGGCTGG TGGCTCACGT TCGGATGCTG GGATTGGATG AGAGAGAAAA ACCTCAGACT 
ATGATTCGTC AGCTCATGAC TCCTATGTCA GGGGAAAACA CTTTGCAGTT TTACCATGAG 
CGTGTGGTGA TCATGAGCTC TATCTTGGAG CACATGTGTG CTGATGTGTT TCAACAGACA 
GGACTGTCTC TGCGACCAGC AGTCGAGGGT CAGGAGCCTA TTCCCTACCG GAACCTTCTC 
CCAGCTCGCC TGCCCATCCG ACAGGCTCTG CAGTCCCAGT TCAGGAAGGT TCTGGAAAAG 
GGCTGGGTGG ACTCGCATGC GTTTCATCTC TTTGAGAACC TGCTACACAT GGGAGGGGTG 
TTCTGGTTTA CTAACAACCT GGTAAAGGAA CTCCTAAAGG AGACGCGTAA AGAGTGGGCG 
TCTCGTGTGG TGGAGTTGCT TTATAGTATT TTCTGCCTGG ACACACAGCA AATCACCCTC 
ACCCTACTGG GTCATATTTT GCCACGTCTC CTCACTGACC CTGCGCACTG GCACAGCCTG 
GCAGACCCAC CCGGCAGAGC CCTTGCAAAG TTATCAGTGT GGTGTGCTCT CAGTTCTTAC 
TCGTCTCATC ATAAGGGTCA AGCATCAGCA CGACAACGCA AAAGACACCG TGAGGATATA 
GAGGACTACA CCAGCCTGTT CCCTTTGGAT GACACACAGC CATCTAAACT GATGCGTTTG 
CTGAGCTCCA ATGAGGACGA GAATTCTATG CTGTCCAGTC CAGGTGACCG TTCCATAAGC 
AGTTCACTGT CTGCCTCTCA GCTCCACACC GTCAACATGA GAGATCCTCT CAACCGAGTG 
CTGGCAAATC TCTTCCTGTT GATTTCCTCT CAGTTGGGGT CTAAGACAGC AGGCCCACAC 
ACGCAGTTTG TTCAGAGCTT TGTTGAGGAG TGTGTGGACT GTTCAGAGCA AGGCAGCCGC 
AGCAGCATCC TGCAGTTTAT GCCCTTTACC ATGATTTCCG AGCTGGTGAA GCTTCAGTCA 
CTGGCTAAAC CCAAAACTGT GCTGTCCATC ACAGACCTGA GCCTTCCTCT GGGCCGGAGA 
ATGGCGGCTA AAGCCATCGC TGCTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034151.1
CDS	1..2970
Translation	MKVVNLKQAILQAWKERWSDYQWAINIKKNCPKGATWDYLNLAEALLEQAMIGPSPNPLILSYLKYAISSQMVSYSSVLTAISKFDDFSRELCVKSLLEIMDMFSNRLSCHGKAEECIGLCRAMLCTVVWLLQGCAWYCERLRDPNDFSALESSLRACLERMANLLHSTKNRALVHIARLEEQASWTNVEQAVLKLTENLSAVTNPTLKESLEECVSLVKSIPQMLCLQCDPPVHTTFPSVHAFIMLEGTMNLTGETQPLVEQLMMIKRMQRIPAPLFVLGIWKACFTGLIESPEGNEELKWTAFTFLKIPQVLLRLKKYPQGEKTQDFTEDVNVAFEYLLNLTPLLDKADQRCNCDCLSLLLQECNKLGLLSEANTATLTAKRSEDREHAPRLKTAENANIQPNPGLILRAEPTVTNILKTVDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFIKLNEFPKHISGEGSKSASVRALLFDISFLMLCHVVQTYGSEVILSDPSPSSETPFFETWLQTCMPEEGKTLNPDHPCFRPESGKVESLVALLNSSSEMKLVQMKWHEICLSTPAAILEVLNAWENGVLSVEAVQKITDNIKGKVCSMAICAVAWLVAHVRMLGLDEREKPQTMIRQLMTPMSGENTLQFYHERVVIMSSILEHMCADVFQQTGLSLRPAVEGQEPIPYRNLLPARLPIRQALQSQFRKVLEKGWVDSHAFHLFENLLHMGGVFWFTNNLVKELLKETRKEWASRVVELLYSIFCLDTQQITLTLLGHILPRLLTDPAHWHSLADPPGRALAKLSVWCALSSYSSHHKGQASARQRKRHREDIEDYTSLFPLDDTQPSKLMRLLSSNEDENSMLSSPGDRSISSSLSASQLHTVNMRDPLNRVLANLFLLISSQLGSKTAGPHTQFVQSFVEECVDCSEQGSRSSILQFMPFTMISELVKLQSLAKPKTVLSITDLSLPLGRRMAAKAIAAL

Target ORF information:

RefSeq Version	NM_001039062.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 24 (med24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039062.1

ORF Insert Sequence:

ATGAAGGTGG TGAATCTGAA ACAGGCCATC CTACAGGCGT GGAAGGAACG TTGGAGTGAT 
TATCAGTGGG CCATTAACAT TAAGAAGAAC TGTCCCAAAG GAGCTACATG GGATTATCTC 
AATCTAGCAG AAGCTCTTCT GGAGCAGGCT ATGATTGGCC CATCTCCCAA CCCCCTGATC 
CTGTCCTATC TGAAGTATGC CATCAGTTCA CAGATGGTGT CGTATTCTAG TGTGTTAACG 
GCAATCAGCA AGTTTGATGA TTTTTCCCGG GAGCTATGTG TCAAATCATT GCTTGAGATT 
ATGGACATGT TCTCCAACCG ACTAAGCTGT CATGGAAAGG CAGAGGAGTG TATTGGGCTC 
TGCCGCGCCA TGCTGTGTAC CGTGGTTTGG CTCCTGCAGG GCTGTGCATG GTACTGTGAG 
AGACTGCGGG ACCCCAATGA TTTTTCTGCA TTAGAAAGCA GTCTGAGGGC CTGCCTGGAG 
CGCATGGCCA ATCTCTTGCA CAGCACAAAG AACAGAGCCC TGGTACACAT CGCACGCCTG 
GAGGAGCAAG CATCCTGGAC CAATGTAGAG CAAGCTGTGC TCAAGCTGAC AGAAAATCTC 
AGTGCTGTGA CCAATCCAAC TCTGAAGGAA AGTCTTGAGG AGTGTGTTTC ATTGGTCAAA 
AGTATTCCTC AGATGTTGTG TCTTCAGTGT GATCCACCAG TCCACACCAC ATTTCCTTCA 
GTCCATGCCT TCATCATGCT GGAGGGAACC ATGAACCTCA CTGGAGAGAC GCAGCCATTA 
GTGGAGCAGC TAATGATGAT CAAGAGGATG CAGCGGATTC CAGCCCCACT GTTTGTGCTG 
GGGATCTGGA AGGCCTGTTT CACTGGACTT ATCGAGTCTC CAGAGGGAAA CGAGGAGCTC 
AAATGGACGG CCTTCACATT CCTAAAGATT CCTCAGGTTT TACTAAGACT AAAAAAGTAC 
CCACAAGGAG AGAAGACGCA GGATTTTACG GAGGATGTGA ATGTGGCTTT TGAATATCTT 
CTGAATCTCA CTCCTCTGCT GGATAAAGCC GATCAGAGAT GCAACTGTGA CTGCTTGAGT 
CTTTTACTGC AGGAGTGTAA TAAACTGGGG CTGCTCTCTG AAGCAAACAC CGCCACTCTT 
ACTGCAAAAC GGAGTGAGGA CCGAGAACAT GCACCTAGAC TGAAGACAGC AGAAAATGCC 
AACATTCAGC CCAATCCAGG CCTCATCCTG AGAGCCGAAC CAACCGTCAC CAACATCCTT 
AAGACCGTGG ATGCGGATCA TTCTAAGTCT CCCGAGGGAT TGCTGGGAGT TTTGGGTCAC 
ATGCTCTCAG GAAAGAGTCT TGATTTGCTA CTGGCCGCTG CTGCAGCCAC TGGAAAACTC 
AAGTCTTTCG CCCGCAAATT CATCAAGCTG AACGAGTTTC CAAAGCACAT TAGTGGAGAA 
GGATCGAAGT CTGCATCAGT ACGAGCTCTG CTATTCGACA TTTCCTTCCT CATGCTGTGT 
CATGTCGTAC AGACGTATGG ATCAGAGGTG ATTCTTTCAG ACCCCAGCCC ATCAAGCGAG 
ACCCCTTTCT TTGAGACATG GCTTCAGACG TGCATGCCAG AGGAAGGGAA GACCCTGAAT 
CCAGACCACC CTTGCTTTAG ACCTGAGTCT GGGAAGGTGG AGAGCCTGGT GGCCCTGCTT 
AATTCTTCAT CTGAGATGAA GCTTGTTCAG ATGAAATGGC ATGAAATATG TCTTAGTACT 
CCTGCAGCTA TACTTGAGGT CCTGAATGCC TGGGAAAATG GAGTCTTGTC AGTGGAAGCT 
GTTCAGAAGA TCACAGATAA TATAAAAGGA AAGGTGTGCA GTATGGCCAT CTGTGCAGTG 
GCATGGCTGG TGGCTCACGT TCGGATGCTG GGATTGGATG AGAGAGAAAA ACCTCAGACT 
ATGATTCGTC AGCTCATGAC TCCTATGTCA GGGGAAAACA CTTTGCAGTT TTACCATGAG 
CGTGTGGTGA TCATGAGCTC TATCTTGGAG CACATGTGTG CTGATGTGTT TCAACAGACA 
GGACTGTCTC TGCGACCAGC AGTCGAGGGT CAGGAGCCTA TTCCCTACCG GAACCTTCTC 
CCAGCTCGCC TGCCCATCCG ACAGGCTCTG CAGTCCCAGT TCAGGAAGGT TCTGGAAAAG 
GGCTGGGTGG ACTCGCATGC GTTTCATCTC TTTGAGAACC TGCTACACAT GGGAGGGGTG 
TTCTGGTTTA CTAACAACCT GGTAAAGGAA CTCCTAAAGG AGACGCGTAA AGAGTGGGCG 
TCTCGTGTGG TGGAGTTGCT TTATAGTATT TTCTGCCTGG ACACACAGCA AATCACCCTC 
ACCCTACTGG GTCATATTTT GCCACGTCTC CTCACTGACC CTGCGCACTG GCACAGCCTG 
GCAGACCCAC CCGGCAGAGC CCTTGCAAAG TTATCAGTGT GGTGTGCTCT CAGTTCTTAC 
TCGTCTCATC ATAAGGGTCA AGCATCAGCA CGACAACGCA AAAGACACCG TGAGGATATA 
GAGGACTACA CCAGCCTGTT CCCTTTGGAT GACACACAGC CATCTAAACT GATGCGTTTG 
CTGAGCTCCA ATGAGGACGA GAATTCTATG CTGTCCAGTC CAGGTGACCG TTCCATAAGC 
AGTTCACTGT CTGCCTCTCA GCTCCACACC GTCAACATGA GAGATCCTCT CAACCGAGTG 
CTGGCAAATC TCTTCCTGTT GATTTCCTCT CAGTTGGGGT CTAAGACAGC AGGCCCACAC 
ACGCAGTTTG TTCAGAGCTT TGTTGAGGAG TGTGTGGACT GTTCAGAGCA AGGCAGCCGC 
AGCAGCATCC TGCAGTTTAT GCCCTTTACC ATGATTTCCG AGCTGGTGAA GCTTCAGTCA 
CTGGCTAAAC CCAAAACTGT GCTGTCCATC ACAGACCTGA GCCTTCCTCT GGGCCGGAGA 
ATGGCGGCTA AAGCCATCGC TGCTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med24 ( XM_009306486.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304761.1 Danio rerio mediator complex subunit 24 (med24), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39777
Clone ID Related Accession (Same CDS sequence)	XM_009306486.2 , XM_009306486.3
Accession Version	XM_009306486.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 24 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGG TGAATCTGAA ACAGGCCATC CTACAGGCGT GGAAGGAACG TTGGAGTGAT 
TATCAGTGGG CCATTAACAT TAAGAAGAAC TGTCCCAAAG GAGCTACATG GGATTATCTC 
AATCTAGCAG AAGCTCTTCT GGAGCAGGCT ATGATTGGCC CATCTCCCAA CCCCCTGATC 
CTGTCCTATC TGAAGTATGC CATCAGTTCA CAGATGGTGT CGTATTCTAG TGTGTTAACG 
GCAATCAGCA AGTTTGATGA TTTTTCACGG GAGCTATGTG TCAAATCATT GCTTGAGATT 
ATGGACATGT TCTCCAACCG ACTAAGCTGT CATGGAAAGG CAGAGGAGTG TATTGGGCTC 
TGCCGCGCCA TGCTGTGTAC CGTGGTTTGG CTCCTGCAGG GCTGTGCATG GTACTGTGAG 
AGACTGCGGG ACCCCAATGA TTTTTCTGCA TTAGAAAGCA GTCTGAGGGC CTGCCTGGAG 
CGCATGGCCA ATCTCTTGCA CAGCACAAAG AACAGAGCCT TGGTACACAT CGCACGCCTG 
GAGGAGCAAG CATCCTGGAC CAATGTAGAG CAAGCTGTGC TCAAGCTGAC AGAAAATCTC 
AGTGCTGTGA CCAATCCAAC TCTGAAGGAA AGTCTTGAGG AGTGTGTTTC ATTGGTCAAA 
AGTATTCCTC AGATGTTGTG TCTTCAGTGT GATCCACCAG TCCACACCAC ATTTCCTTCA 
GTCCATGCCT TCATCATGCT GGAGGGAACC ATGAACCTCA CTGGAGAGAC GCAGCCATTA 
GTGGAGCAGC TAATGATGAT CAAGAGGATG CAGCGGATTC CAGCTCCACT GTTTGTGCTG 
GAGATCTGGA AGGCCTGTTT CACTGGACTT ATCGAGTCCC CAGAGGGAAA CGAGGAGCTC 
AAATGGACTG CCTTCACATT CCTAAAGATT CCTCAAGTTT TACTAAGACT AAAAAAGTAC 
CCACAAGGAG AGAAGACGCA GGATTTTACG GAGGATGTGA ATGTGGCTTT TGAATATCTT 
CTGAATCTCA CTCCTTTGCT GGATAAAGCT GATCAGAGAT GCAACTGTGA CTGCTTGAGT 
CTTTTACTGC AGGAGTGTAA TAAACTGGGG CTGCTCTCTG AAGCAAACAC CGCCACTCTT 
ACTGCAAAAC GGAGTGAGGA CCGAGAACAT GCACCTAGAC TGAAGACAGC AGAAAATGCC 
AACATTCAGC CCAATCCAGG CCTCATCTTG AGAGCCGAAC CAACTGTCAC CAACATCCTT 
AAGACCGTGG ATGCGGATCA TTCTAAGTCT CCCGAGGGAT TGCTGGGAGT TTTGGGTCAC 
ATGCTCTCAG GAAAGAGTCT TGATTTGCTA CTGGCCGCTG CTGCAGCCAC TGGAAAACTC 
AAGTCTTTCG CCCGCAAATT CATCAAGCTG AACGAGTTTC CAAAGCACAT TAGTGGAGAA 
GGATCGAAGT CTGCATCAGT ACGAGCTCTG CTATTCGACA TTTCCTTCCT CATGCTGTGT 
CATGTCGTAC AGACGTATGG ATCAGAGGTG ATTCTTTCAG ACCCCAGCCC ATCAAGCGAG 
ACCCCTTTCT TTGAGACATG GCTTCAGACG TGCATGCCAG AGGAAGGGAA GACCCTGAAT 
CCAGACCACC CTTGCTTTAG ACCTGAGTCT GGGAAGGTGG AGAGCCTGGT GGCCCTGCTT 
AATTCTTCAT CTGAGATGAA GCTTGTTCAG ATGAAATGGC ATGAAATATG TCTTAGTACT 
CCTGCAGCTA TACTTGAGGT CCTGAATGCC TGGGAAAATG GAGTTTTGTC AGTGGAGGCT 
GTTCAGAAGA TCACAGATAA TATAAAAGGA AAGGTGTGCA GTATGGCCAT CTGTGCAGTG 
GCATGGCTGG TGGCTCACGT TCGGATGCTG GGATTGGATG AGAGAGAAAA ACCTCAGACT 
ATGATTCGTC AGCTCATGAC TCCTATGTCA GGGGAAAACA CTTTGCAGTT TTACCATGAG 
CGTGTGGTGA TCATGAGCTC TATCTTGGAG CACATGTGTG CTGATGTGTT TCAACAAACA 
GGACTGTCTC TGCGACCAGC AGTCGAGGGT CAGGAGCCAA TTCCCTACCG GAACCTTCTC 
CCAGCTCGCC TGCCCATCCG ACAGGCTCTG CAGTCCCAGT TCAGAAAGGT TCTGGAAAAG 
GGCTGGGTGG ACTCGCATGC GTTTCATCTC TTTGAGAACC TGCTACACAT GGGAGGGGTG 
TTCTGGTTTA CTAACAACCT GGTAAAGGAA CTCTTAAAGG AGACGCGTAA AGAGTGGGCG 
TCTCGTGTGG TGGAGTTGCT TTATAGTATT TTCTGCCTGG ACACACAGCA AATCACCCTC 
ACTCTACTGG GTCATATTTT GCCACGTCTC CTCACTGACC CTGCACACTG GCACAGCCTG 
GCAGACCCAC CCGGCAGAGC CCTTGCAAAG TTATCAGTGT GGTGTGCTCT CAGTTCTTAC 
TCGTCTCATC ATAAGGGTCA AGCATCAGCA CGACAACGCA AAAGACACCG TGAGGATATA 
GAGGACTACA CCAGCCTGTT CCCTTTGGAT GACACACAGC CATCTAAACT GATGCGTTTG 
CTGAGCTCCA ATGAGGACGA GAATTCTATG CTGTCCAGTC CAGGTGACCG TTCCATAAGC 
AGTTCACTGT CTGCCTCTCA GCTCCACACC GTCAACATGA GAGATCCTCT CAACCGAGTG 
CTGGCAAATC TCTTCCTGTT GATTTCCTCT CAGTTGGGGT CTAAGACAGC AGGCCCACAC 
ACGCAGTTTG TTCAGAGCTT TGTTGAGGAG TGTGTGGACT GTTCAGAGCA AGGCAGCCGC 
AGCAGCATCC TGCAGTTTAT GCCCTTTACC ATGATTTCCG AGCTGGTGAA GCTTCAGTCA 
CTGGCTAAAC CTAAAACTGT GCTGTCCATC ACAGACCTGA GCCTTCCTCT GGGCCGGAGA 
ATGGCGGCTA AAGCCATCGC TGCTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304761.1
CDS	113..3082
Translation	MKVVNLKQAILQAWKERWSDYQWAINIKKNCPKGATWDYLNLAEALLEQAMIGPSPNPLILSYLKYAISSQMVSYSSVLTAISKFDDFSRELCVKSLLEIMDMFSNRLSCHGKAEECIGLCRAMLCTVVWLLQGCAWYCERLRDPNDFSALESSLRACLERMANLLHSTKNRALVHIARLEEQASWTNVEQAVLKLTENLSAVTNPTLKESLEECVSLVKSIPQMLCLQCDPPVHTTFPSVHAFIMLEGTMNLTGETQPLVEQLMMIKRMQRIPAPLFVLEIWKACFTGLIESPEGNEELKWTAFTFLKIPQVLLRLKKYPQGEKTQDFTEDVNVAFEYLLNLTPLLDKADQRCNCDCLSLLLQECNKLGLLSEANTATLTAKRSEDREHAPRLKTAENANIQPNPGLILRAEPTVTNILKTVDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFIKLNEFPKHISGEGSKSASVRALLFDISFLMLCHVVQTYGSEVILSDPSPSSETPFFETWLQTCMPEEGKTLNPDHPCFRPESGKVESLVALLNSSSEMKLVQMKWHEICLSTPAAILEVLNAWENGVLSVEAVQKITDNIKGKVCSMAICAVAWLVAHVRMLGLDEREKPQTMIRQLMTPMSGENTLQFYHERVVIMSSILEHMCADVFQQTGLSLRPAVEGQEPIPYRNLLPARLPIRQALQSQFRKVLEKGWVDSHAFHLFENLLHMGGVFWFTNNLVKELLKETRKEWASRVVELLYSIFCLDTQQITLTLLGHILPRLLTDPAHWHSLADPPGRALAKLSVWCALSSYSSHHKGQASARQRKRHREDIEDYTSLFPLDDTQPSKLMRLLSSNEDENSMLSSPGDRSISSSLSASQLHTVNMRDPLNRVLANLFLLISSQLGSKTAGPHTQFVQSFVEECVDCSEQGSRSSILQFMPFTMISELVKLQSLAKPKTVLSITDLSLPLGRRMAAKAIAAL

Target ORF information:

RefSeq Version	XM_009306486.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 24 (med24), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306486.2

ORF Insert Sequence:

ATGAAGGTGG TGAATCTGAA ACAGGCCATC CTACAGGCGT GGAAGGAACG TTGGAGTGAT 
TATCAGTGGG CCATTAACAT TAAGAAGAAC TGTCCCAAAG GAGCTACATG GGATTATCTC 
AATCTAGCAG AAGCTCTTCT GGAGCAGGCT ATGATTGGCC CATCTCCCAA CCCCCTGATC 
CTGTCCTATC TGAAGTATGC CATCAGTTCA CAGATGGTGT CGTATTCTAG TGTGTTAACG 
GCAATCAGCA AGTTTGATGA TTTTTCACGG GAGCTATGTG TCAAATCATT GCTTGAGATT 
ATGGACATGT TCTCCAACCG ACTAAGCTGT CATGGAAAGG CAGAGGAGTG TATTGGGCTC 
TGCCGCGCCA TGCTGTGTAC CGTGGTTTGG CTCCTGCAGG GCTGTGCATG GTACTGTGAG 
AGACTGCGGG ACCCCAATGA TTTTTCTGCA TTAGAAAGCA GTCTGAGGGC CTGCCTGGAG 
CGCATGGCCA ATCTCTTGCA CAGCACAAAG AACAGAGCCT TGGTACACAT CGCACGCCTG 
GAGGAGCAAG CATCCTGGAC CAATGTAGAG CAAGCTGTGC TCAAGCTGAC AGAAAATCTC 
AGTGCTGTGA CCAATCCAAC TCTGAAGGAA AGTCTTGAGG AGTGTGTTTC ATTGGTCAAA 
AGTATTCCTC AGATGTTGTG TCTTCAGTGT GATCCACCAG TCCACACCAC ATTTCCTTCA 
GTCCATGCCT TCATCATGCT GGAGGGAACC ATGAACCTCA CTGGAGAGAC GCAGCCATTA 
GTGGAGCAGC TAATGATGAT CAAGAGGATG CAGCGGATTC CAGCTCCACT GTTTGTGCTG 
GAGATCTGGA AGGCCTGTTT CACTGGACTT ATCGAGTCCC CAGAGGGAAA CGAGGAGCTC 
AAATGGACTG CCTTCACATT CCTAAAGATT CCTCAAGTTT TACTAAGACT AAAAAAGTAC 
CCACAAGGAG AGAAGACGCA GGATTTTACG GAGGATGTGA ATGTGGCTTT TGAATATCTT 
CTGAATCTCA CTCCTTTGCT GGATAAAGCT GATCAGAGAT GCAACTGTGA CTGCTTGAGT 
CTTTTACTGC AGGAGTGTAA TAAACTGGGG CTGCTCTCTG AAGCAAACAC CGCCACTCTT 
ACTGCAAAAC GGAGTGAGGA CCGAGAACAT GCACCTAGAC TGAAGACAGC AGAAAATGCC 
AACATTCAGC CCAATCCAGG CCTCATCTTG AGAGCCGAAC CAACTGTCAC CAACATCCTT 
AAGACCGTGG ATGCGGATCA TTCTAAGTCT CCCGAGGGAT TGCTGGGAGT TTTGGGTCAC 
ATGCTCTCAG GAAAGAGTCT TGATTTGCTA CTGGCCGCTG CTGCAGCCAC TGGAAAACTC 
AAGTCTTTCG CCCGCAAATT CATCAAGCTG AACGAGTTTC CAAAGCACAT TAGTGGAGAA 
GGATCGAAGT CTGCATCAGT ACGAGCTCTG CTATTCGACA TTTCCTTCCT CATGCTGTGT 
CATGTCGTAC AGACGTATGG ATCAGAGGTG ATTCTTTCAG ACCCCAGCCC ATCAAGCGAG 
ACCCCTTTCT TTGAGACATG GCTTCAGACG TGCATGCCAG AGGAAGGGAA GACCCTGAAT 
CCAGACCACC CTTGCTTTAG ACCTGAGTCT GGGAAGGTGG AGAGCCTGGT GGCCCTGCTT 
AATTCTTCAT CTGAGATGAA GCTTGTTCAG ATGAAATGGC ATGAAATATG TCTTAGTACT 
CCTGCAGCTA TACTTGAGGT CCTGAATGCC TGGGAAAATG GAGTTTTGTC AGTGGAGGCT 
GTTCAGAAGA TCACAGATAA TATAAAAGGA AAGGTGTGCA GTATGGCCAT CTGTGCAGTG 
GCATGGCTGG TGGCTCACGT TCGGATGCTG GGATTGGATG AGAGAGAAAA ACCTCAGACT 
ATGATTCGTC AGCTCATGAC TCCTATGTCA GGGGAAAACA CTTTGCAGTT TTACCATGAG 
CGTGTGGTGA TCATGAGCTC TATCTTGGAG CACATGTGTG CTGATGTGTT TCAACAAACA 
GGACTGTCTC TGCGACCAGC AGTCGAGGGT CAGGAGCCAA TTCCCTACCG GAACCTTCTC 
CCAGCTCGCC TGCCCATCCG ACAGGCTCTG CAGTCCCAGT TCAGAAAGGT TCTGGAAAAG 
GGCTGGGTGG ACTCGCATGC GTTTCATCTC TTTGAGAACC TGCTACACAT GGGAGGGGTG 
TTCTGGTTTA CTAACAACCT GGTAAAGGAA CTCTTAAAGG AGACGCGTAA AGAGTGGGCG 
TCTCGTGTGG TGGAGTTGCT TTATAGTATT TTCTGCCTGG ACACACAGCA AATCACCCTC 
ACTCTACTGG GTCATATTTT GCCACGTCTC CTCACTGACC CTGCACACTG GCACAGCCTG 
GCAGACCCAC CCGGCAGAGC CCTTGCAAAG TTATCAGTGT GGTGTGCTCT CAGTTCTTAC 
TCGTCTCATC ATAAGGGTCA AGCATCAGCA CGACAACGCA AAAGACACCG TGAGGATATA 
GAGGACTACA CCAGCCTGTT CCCTTTGGAT GACACACAGC CATCTAAACT GATGCGTTTG 
CTGAGCTCCA ATGAGGACGA GAATTCTATG CTGTCCAGTC CAGGTGACCG TTCCATAAGC 
AGTTCACTGT CTGCCTCTCA GCTCCACACC GTCAACATGA GAGATCCTCT CAACCGAGTG 
CTGGCAAATC TCTTCCTGTT GATTTCCTCT CAGTTGGGGT CTAAGACAGC AGGCCCACAC 
ACGCAGTTTG TTCAGAGCTT TGTTGAGGAG TGTGTGGACT GTTCAGAGCA AGGCAGCCGC 
AGCAGCATCC TGCAGTTTAT GCCCTTTACC ATGATTTCCG AGCTGGTGAA GCTTCAGTCA 
CTGGCTAAAC CTAAAACTGT GCTGTCCATC ACAGACCTGA GCCTTCCTCT GGGCCGGAGA 
ATGGCGGCTA AAGCCATCGC TGCTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med24 ( XM_009306486.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304761.1 Danio rerio mediator complex subunit 24 (med24), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39777
Clone ID Related Accession (Same CDS sequence)	XM_009306486.2 , XM_009306486.3
Accession Version	XM_009306486.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 24 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306486.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGG TGAATCTGAA ACAGGCCATC CTACAGGCGT GGAAGGAACG TTGGAGTGAT 
TATCAGTGGG CCATTAACAT TAAGAAGAAC TGTCCCAAAG GAGCTACATG GGATTATCTC 
AATCTAGCAG AAGCTCTTCT GGAGCAGGCT ATGATTGGCC CATCTCCCAA CCCCCTGATC 
CTGTCCTATC TGAAGTATGC CATCAGTTCA CAGATGGTGT CGTATTCTAG TGTGTTAACG 
GCAATCAGCA AGTTTGATGA TTTTTCACGG GAGCTATGTG TCAAATCATT GCTTGAGATT 
ATGGACATGT TCTCCAACCG ACTAAGCTGT CATGGAAAGG CAGAGGAGTG TATTGGGCTC 
TGCCGCGCCA TGCTGTGTAC CGTGGTTTGG CTCCTGCAGG GCTGTGCATG GTACTGTGAG 
AGACTGCGGG ACCCCAATGA TTTTTCTGCA TTAGAAAGCA GTCTGAGGGC CTGCCTGGAG 
CGCATGGCCA ATCTCTTGCA CAGCACAAAG AACAGAGCCT TGGTACACAT CGCACGCCTG 
GAGGAGCAAG CATCCTGGAC CAATGTAGAG CAAGCTGTGC TCAAGCTGAC AGAAAATCTC 
AGTGCTGTGA CCAATCCAAC TCTGAAGGAA AGTCTTGAGG AGTGTGTTTC ATTGGTCAAA 
AGTATTCCTC AGATGTTGTG TCTTCAGTGT GATCCACCAG TCCACACCAC ATTTCCTTCA 
GTCCATGCCT TCATCATGCT GGAGGGAACC ATGAACCTCA CTGGAGAGAC GCAGCCATTA 
GTGGAGCAGC TAATGATGAT CAAGAGGATG CAGCGGATTC CAGCTCCACT GTTTGTGCTG 
GAGATCTGGA AGGCCTGTTT CACTGGACTT ATCGAGTCCC CAGAGGGAAA CGAGGAGCTC 
AAATGGACTG CCTTCACATT CCTAAAGATT CCTCAAGTTT TACTAAGACT AAAAAAGTAC 
CCACAAGGAG AGAAGACGCA GGATTTTACG GAGGATGTGA ATGTGGCTTT TGAATATCTT 
CTGAATCTCA CTCCTTTGCT GGATAAAGCT GATCAGAGAT GCAACTGTGA CTGCTTGAGT 
CTTTTACTGC AGGAGTGTAA TAAACTGGGG CTGCTCTCTG AAGCAAACAC CGCCACTCTT 
ACTGCAAAAC GGAGTGAGGA CCGAGAACAT GCACCTAGAC TGAAGACAGC AGAAAATGCC 
AACATTCAGC CCAATCCAGG CCTCATCTTG AGAGCCGAAC CAACTGTCAC CAACATCCTT 
AAGACCGTGG ATGCGGATCA TTCTAAGTCT CCCGAGGGAT TGCTGGGAGT TTTGGGTCAC 
ATGCTCTCAG GAAAGAGTCT TGATTTGCTA CTGGCCGCTG CTGCAGCCAC TGGAAAACTC 
AAGTCTTTCG CCCGCAAATT CATCAAGCTG AACGAGTTTC CAAAGCACAT TAGTGGAGAA 
GGATCGAAGT CTGCATCAGT ACGAGCTCTG CTATTCGACA TTTCCTTCCT CATGCTGTGT 
CATGTCGTAC AGACGTATGG ATCAGAGGTG ATTCTTTCAG ACCCCAGCCC ATCAAGCGAG 
ACCCCTTTCT TTGAGACATG GCTTCAGACG TGCATGCCAG AGGAAGGGAA GACCCTGAAT 
CCAGACCACC CTTGCTTTAG ACCTGAGTCT GGGAAGGTGG AGAGCCTGGT GGCCCTGCTT 
AATTCTTCAT CTGAGATGAA GCTTGTTCAG ATGAAATGGC ATGAAATATG TCTTAGTACT 
CCTGCAGCTA TACTTGAGGT CCTGAATGCC TGGGAAAATG GAGTTTTGTC AGTGGAGGCT 
GTTCAGAAGA TCACAGATAA TATAAAAGGA AAGGTGTGCA GTATGGCCAT CTGTGCAGTG 
GCATGGCTGG TGGCTCACGT TCGGATGCTG GGATTGGATG AGAGAGAAAA ACCTCAGACT 
ATGATTCGTC AGCTCATGAC TCCTATGTCA GGGGAAAACA CTTTGCAGTT TTACCATGAG 
CGTGTGGTGA TCATGAGCTC TATCTTGGAG CACATGTGTG CTGATGTGTT TCAACAAACA 
GGACTGTCTC TGCGACCAGC AGTCGAGGGT CAGGAGCCAA TTCCCTACCG GAACCTTCTC 
CCAGCTCGCC TGCCCATCCG ACAGGCTCTG CAGTCCCAGT TCAGAAAGGT TCTGGAAAAG 
GGCTGGGTGG ACTCGCATGC GTTTCATCTC TTTGAGAACC TGCTACACAT GGGAGGGGTG 
TTCTGGTTTA CTAACAACCT GGTAAAGGAA CTCTTAAAGG AGACGCGTAA AGAGTGGGCG 
TCTCGTGTGG TGGAGTTGCT TTATAGTATT TTCTGCCTGG ACACACAGCA AATCACCCTC 
ACTCTACTGG GTCATATTTT GCCACGTCTC CTCACTGACC CTGCACACTG GCACAGCCTG 
GCAGACCCAC CCGGCAGAGC CCTTGCAAAG TTATCAGTGT GGTGTGCTCT CAGTTCTTAC 
TCGTCTCATC ATAAGGGTCA AGCATCAGCA CGACAACGCA AAAGACACCG TGAGGATATA 
GAGGACTACA CCAGCCTGTT CCCTTTGGAT GACACACAGC CATCTAAACT GATGCGTTTG 
CTGAGCTCCA ATGAGGACGA GAATTCTATG CTGTCCAGTC CAGGTGACCG TTCCATAAGC 
AGTTCACTGT CTGCCTCTCA GCTCCACACC GTCAACATGA GAGATCCTCT CAACCGAGTG 
CTGGCAAATC TCTTCCTGTT GATTTCCTCT CAGTTGGGGT CTAAGACAGC AGGCCCACAC 
ACGCAGTTTG TTCAGAGCTT TGTTGAGGAG TGTGTGGACT GTTCAGAGCA AGGCAGCCGC 
AGCAGCATCC TGCAGTTTAT GCCCTTTACC ATGATTTCCG AGCTGGTGAA GCTTCAGTCA 
CTGGCTAAAC CTAAAACTGT GCTGTCCATC ACAGACCTGA GCCTTCCTCT GGGCCGGAGA 
ATGGCGGCTA AAGCCATCGC TGCTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304761.1
CDS	110..3079
Translation	MKVVNLKQAILQAWKERWSDYQWAINIKKNCPKGATWDYLNLAEALLEQAMIGPSPNPLILSYLKYAISSQMVSYSSVLTAISKFDDFSRELCVKSLLEIMDMFSNRLSCHGKAEECIGLCRAMLCTVVWLLQGCAWYCERLRDPNDFSALESSLRACLERMANLLHSTKNRALVHIARLEEQASWTNVEQAVLKLTENLSAVTNPTLKESLEECVSLVKSIPQMLCLQCDPPVHTTFPSVHAFIMLEGTMNLTGETQPLVEQLMMIKRMQRIPAPLFVLEIWKACFTGLIESPEGNEELKWTAFTFLKIPQVLLRLKKYPQGEKTQDFTEDVNVAFEYLLNLTPLLDKADQRCNCDCLSLLLQECNKLGLLSEANTATLTAKRSEDREHAPRLKTAENANIQPNPGLILRAEPTVTNILKTVDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFIKLNEFPKHISGEGSKSASVRALLFDISFLMLCHVVQTYGSEVILSDPSPSSETPFFETWLQTCMPEEGKTLNPDHPCFRPESGKVESLVALLNSSSEMKLVQMKWHEICLSTPAAILEVLNAWENGVLSVEAVQKITDNIKGKVCSMAICAVAWLVAHVRMLGLDEREKPQTMIRQLMTPMSGENTLQFYHERVVIMSSILEHMCADVFQQTGLSLRPAVEGQEPIPYRNLLPARLPIRQALQSQFRKVLEKGWVDSHAFHLFENLLHMGGVFWFTNNLVKELLKETRKEWASRVVELLYSIFCLDTQQITLTLLGHILPRLLTDPAHWHSLADPPGRALAKLSVWCALSSYSSHHKGQASARQRKRHREDIEDYTSLFPLDDTQPSKLMRLLSSNEDENSMLSSPGDRSISSSLSASQLHTVNMRDPLNRVLANLFLLISSQLGSKTAGPHTQFVQSFVEECVDCSEQGSRSSILQFMPFTMISELVKLQSLAKPKTVLSITDLSLPLGRRMAAKAIAAL

Target ORF information:

RefSeq Version	XM_009306486.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 24 (med24), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306486.3

ORF Insert Sequence:

ATGAAGGTGG TGAATCTGAA ACAGGCCATC CTACAGGCGT GGAAGGAACG TTGGAGTGAT 
TATCAGTGGG CCATTAACAT TAAGAAGAAC TGTCCCAAAG GAGCTACATG GGATTATCTC 
AATCTAGCAG AAGCTCTTCT GGAGCAGGCT ATGATTGGCC CATCTCCCAA CCCCCTGATC 
CTGTCCTATC TGAAGTATGC CATCAGTTCA CAGATGGTGT CGTATTCTAG TGTGTTAACG 
GCAATCAGCA AGTTTGATGA TTTTTCACGG GAGCTATGTG TCAAATCATT GCTTGAGATT 
ATGGACATGT TCTCCAACCG ACTAAGCTGT CATGGAAAGG CAGAGGAGTG TATTGGGCTC 
TGCCGCGCCA TGCTGTGTAC CGTGGTTTGG CTCCTGCAGG GCTGTGCATG GTACTGTGAG 
AGACTGCGGG ACCCCAATGA TTTTTCTGCA TTAGAAAGCA GTCTGAGGGC CTGCCTGGAG 
CGCATGGCCA ATCTCTTGCA CAGCACAAAG AACAGAGCCT TGGTACACAT CGCACGCCTG 
GAGGAGCAAG CATCCTGGAC CAATGTAGAG CAAGCTGTGC TCAAGCTGAC AGAAAATCTC 
AGTGCTGTGA CCAATCCAAC TCTGAAGGAA AGTCTTGAGG AGTGTGTTTC ATTGGTCAAA 
AGTATTCCTC AGATGTTGTG TCTTCAGTGT GATCCACCAG TCCACACCAC ATTTCCTTCA 
GTCCATGCCT TCATCATGCT GGAGGGAACC ATGAACCTCA CTGGAGAGAC GCAGCCATTA 
GTGGAGCAGC TAATGATGAT CAAGAGGATG CAGCGGATTC CAGCTCCACT GTTTGTGCTG 
GAGATCTGGA AGGCCTGTTT CACTGGACTT ATCGAGTCCC CAGAGGGAAA CGAGGAGCTC 
AAATGGACTG CCTTCACATT CCTAAAGATT CCTCAAGTTT TACTAAGACT AAAAAAGTAC 
CCACAAGGAG AGAAGACGCA GGATTTTACG GAGGATGTGA ATGTGGCTTT TGAATATCTT 
CTGAATCTCA CTCCTTTGCT GGATAAAGCT GATCAGAGAT GCAACTGTGA CTGCTTGAGT 
CTTTTACTGC AGGAGTGTAA TAAACTGGGG CTGCTCTCTG AAGCAAACAC CGCCACTCTT 
ACTGCAAAAC GGAGTGAGGA CCGAGAACAT GCACCTAGAC TGAAGACAGC AGAAAATGCC 
AACATTCAGC CCAATCCAGG CCTCATCTTG AGAGCCGAAC CAACTGTCAC CAACATCCTT 
AAGACCGTGG ATGCGGATCA TTCTAAGTCT CCCGAGGGAT TGCTGGGAGT TTTGGGTCAC 
ATGCTCTCAG GAAAGAGTCT TGATTTGCTA CTGGCCGCTG CTGCAGCCAC TGGAAAACTC 
AAGTCTTTCG CCCGCAAATT CATCAAGCTG AACGAGTTTC CAAAGCACAT TAGTGGAGAA 
GGATCGAAGT CTGCATCAGT ACGAGCTCTG CTATTCGACA TTTCCTTCCT CATGCTGTGT 
CATGTCGTAC AGACGTATGG ATCAGAGGTG ATTCTTTCAG ACCCCAGCCC ATCAAGCGAG 
ACCCCTTTCT TTGAGACATG GCTTCAGACG TGCATGCCAG AGGAAGGGAA GACCCTGAAT 
CCAGACCACC CTTGCTTTAG ACCTGAGTCT GGGAAGGTGG AGAGCCTGGT GGCCCTGCTT 
AATTCTTCAT CTGAGATGAA GCTTGTTCAG ATGAAATGGC ATGAAATATG TCTTAGTACT 
CCTGCAGCTA TACTTGAGGT CCTGAATGCC TGGGAAAATG GAGTTTTGTC AGTGGAGGCT 
GTTCAGAAGA TCACAGATAA TATAAAAGGA AAGGTGTGCA GTATGGCCAT CTGTGCAGTG 
GCATGGCTGG TGGCTCACGT TCGGATGCTG GGATTGGATG AGAGAGAAAA ACCTCAGACT 
ATGATTCGTC AGCTCATGAC TCCTATGTCA GGGGAAAACA CTTTGCAGTT TTACCATGAG 
CGTGTGGTGA TCATGAGCTC TATCTTGGAG CACATGTGTG CTGATGTGTT TCAACAAACA 
GGACTGTCTC TGCGACCAGC AGTCGAGGGT CAGGAGCCAA TTCCCTACCG GAACCTTCTC 
CCAGCTCGCC TGCCCATCCG ACAGGCTCTG CAGTCCCAGT TCAGAAAGGT TCTGGAAAAG 
GGCTGGGTGG ACTCGCATGC GTTTCATCTC TTTGAGAACC TGCTACACAT GGGAGGGGTG 
TTCTGGTTTA CTAACAACCT GGTAAAGGAA CTCTTAAAGG AGACGCGTAA AGAGTGGGCG 
TCTCGTGTGG TGGAGTTGCT TTATAGTATT TTCTGCCTGG ACACACAGCA AATCACCCTC 
ACTCTACTGG GTCATATTTT GCCACGTCTC CTCACTGACC CTGCACACTG GCACAGCCTG 
GCAGACCCAC CCGGCAGAGC CCTTGCAAAG TTATCAGTGT GGTGTGCTCT CAGTTCTTAC 
TCGTCTCATC ATAAGGGTCA AGCATCAGCA CGACAACGCA AAAGACACCG TGAGGATATA 
GAGGACTACA CCAGCCTGTT CCCTTTGGAT GACACACAGC CATCTAAACT GATGCGTTTG 
CTGAGCTCCA ATGAGGACGA GAATTCTATG CTGTCCAGTC CAGGTGACCG TTCCATAAGC 
AGTTCACTGT CTGCCTCTCA GCTCCACACC GTCAACATGA GAGATCCTCT CAACCGAGTG 
CTGGCAAATC TCTTCCTGTT GATTTCCTCT CAGTTGGGGT CTAAGACAGC AGGCCCACAC 
ACGCAGTTTG TTCAGAGCTT TGTTGAGGAG TGTGTGGACT GTTCAGAGCA AGGCAGCCGC 
AGCAGCATCC TGCAGTTTAT GCCCTTTACC ATGATTTCCG AGCTGGTGAA GCTTCAGTCA 
CTGGCTAAAC CTAAAACTGT GCTGTCCATC ACAGACCTGA GCCTTCCTCT GGGCCGGAGA 
ATGGCGGCTA AAGCCATCGC TGCTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

The zebrafish mutant lessen: an experimental model for congenital enteric neuropathies.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society28(3)345-57(2016 Mar)
Uyttebroek L,Shepherd IT,Vanden Berghe P,Hubens G,Timmermans JP,Van Nassauw L

In vivo visualization of the development of the enteric nervous system using a Tg(-8.3bphox2b:Kaede) transgenic zebrafish.
Genesis (New York, N.Y. : 2000)52(12)985-90(2014 Dec)
Harrison C,Wabbersen T,Shepherd IT

Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration.
Blood113(11)2535-46(2009 Mar)
Liongue C,Hall CJ,O'Connell BA,Crosier P,Ward AC

Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development.
Genetics174(2)693-705(2006 Oct)
Dürr K,Holzschuh J,Filippi A,Ettl AK,Ryu S,Shepherd IT,Driever W

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

med24 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM