MED24 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED24
Entrez Gene ID	38914
Full Name	Mediator complex subunit 24
Synonyms	CG7999,Dmel\CG7999,Med24,Trap100,dTRAP100,l(3)psg5,med24,psg5
General protein information	Preferred Names Mediator complex subunit 24 Names CG7999 gene product from transcript CG7999-RA CG7999-PA MED24-PA mediator mediator complex subunit 24
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66B11-66B12\|3-34 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_139928.5	NP_648185.1	mediator complex subunit 24

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MED24	XP_005220831.1
Anopheles gambiae (African malaria mosquito)	MED24_ANOGA	XP_315936.4
Macaca mulatta (Rhesus monkey)	MED24	XP_001087996.2
Mus musculus (house mouse)	Med24	NP_035999.2
Homo sapiens (human)	MED24	NP_055630.2
Pan troglodytes (chimpanzee)	MED24	XP_001171125.2
Canis lupus familiaris (dog)	MED24	XP_863448.2
Danio rerio (zebrafish)	med24	NP_001034151.1
Drosophila melanogaster (fruit fly)	MED24	NP_648185.1
Rattus norvegicus (Norway rat)	Med24	NP_001029251.1
Gallus gallus (chicken)	MED24	NP_001026534.1
Xenopus tropicalis (tropical clawed frog)	LOC100491557	XP_004911860.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20703	NM_139928.5 Latest version!	Drosophila melanogaster mediator complex subunit 24 (MED24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED24 ( NM_139928.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648185.1 Drosophila melanogaster mediator complex subunit 24 (MED24), mRNA.

Price & Availability↑

CloneID	OFa20703
Clone ID Related Accession (Same CDS sequence)	NM_139928.5
Accession Version	NM_139928.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139928.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGAGA ACAACAAGAT CCTCCAACTC ATCTATGTGG CGTGGCGCGA GCGCTGGACG 
GACTCCCAGT GGGGCATCAA CATAAAAAAG GTGCTGCCGA GAGGAGTCAG TGGAGATGTA 
TACAATCTGG CTGACTGTCT GATGCAGCAG GCACTCATTG GTTCCACGGC TAATCCGTTG 
GTTCTTAACT ACCTGAAGCA TTCACTGTGC GCCCATCTCG TTTCTCACGC TGCTGTTATT 
CGGTGCATTG CCAAATATGA CAAATTAGAG CGCGTTTATT GCATTACAGC GCTCCTTGAA 
TTCCTGGCCA GCATCGTGGA TGGCGTTACC TGCCGAATAA AATCTGAGGA AGCGGTTTTA 
CCGAGTTCCG TCGTCCACTT GGTGTACTGG CTGCTTCAAA TCTTCGCCAG AACTGTGCAG 
CACTATGAAC TGTATGGTGA GATTAGTGCT GAGCAATCAT ACATGCTGGA CCAGACCTGC 
GTGGTGATAG ATCGGCTTAG CCAGCAGCAG TTCCTGCTGT CTATGCTGTA CGTGGGCTGT 
CACGAAGAGC TCGAAATATG TGGACGGATT CGGGATAAGT ACGCTACCAT CAAGGGATCG 
TTGACGAACT CAAACTTTAC GCTAAATGCA CCACAAGTGG AGCAGCAATT GCAGCAGTTG 
GCCTTTATAG AAGCCAAGCA TCTTGAAATG CAACCACTTA ATCCGCCGTC AACACTCGAG 
AAGATCTCGT GCTGCGTGCA ACCCTTACTG GCGGTGGAAG TGCTTCTCAA TCCGTGCAAG 
GACACCAGCT ACTACGTGGC TGAGTTGCAG ATGCTGCAAC GATTAAAGAA ATACTCCAAT 
ACACGGCTCT TCTACGAAAT CATCCGTGCT GGATTCCTCA CGCTGAGCAA TGTTGCGGAT 
ACAAGTCCTG ACACTATGTG GGGTGCCTTT ATGTTCTTTA AAATGCCGCA CATCATTAAG 
CAATTGCACG CCCTGCAACG GATTCCCGGG GAGCAACCAC CACCGGCCGA TTACATACCC 
GAGTTGGTGG AGGCTTTGGA ATTGCTCATC GAGGACAATC TGCTGCTAGA CTTCATGGAT 
ACTAAGTGCT CATGCAATAT GATCGAGTTC CTTTTGAATG ACTGGACCAA GCAACAACTA 
GTCAATGATG TCCACGTAAA GAAGTTCGCC AGTCAAAGGG AAGCCGCCTC TCAATTACTC 
AAAAAATGCG ACAATGGCCA ACAGACACCC TCCAATATAA ACTTCATCAA GCGCGCAGAG 
GTTCCGCTTT CCGGAGTACT AAAAACGCTG TGCACCAACA AGGTCCAGGA CATGGTTAAT 
GTCCTTTGCC AAGTGCCGGT GGGCAACAGC TTCGAGTTGA TTCTTTCAGT GGCTACCGTC 
GAGGGACGCC TCAAGACATT CGTATCGCGT CTGATCCAGT GCAATGAGAA CTCCAAGCCG 
GTGCCGGGCG ACCTGGGCAA GCTATGCGTC ATCCGGTCCA CTCTGTTTGA TGTTTCCTTC 
CTAATGTTGA CAAGCATTGT GCAGACCTAT GGATCCGATG TGGTCTTATC TGAGCGTGGT 
GACTCATTTT TTGAAAAATG GGTGCGTGAG TGCATGGTGG AAAGGAATAA GCTTAAGAAT 
CCGCGTCAAA TTCTTGCCCT CTGTGATGAC AGTATTGTCG ACGAGCTTTT GCTTAGCTTC 
AGTAAACCAG AGGCATCACA ACTTAAGCCA AACAACTTAA GTTGGCAGGA GACTTGCCTT 
AATCTGCCCG GAGTGCTTCA TCATGTGCTA ATAGCTTGGG AGCAGGAAAC GCTATCCTCG 
GCGGACGTTA AAAGTATTCT GGACAATATT AAGAGAAGAC TTTTCAGTTT TTCCGTTTGC 
GCCACTAGTT TTCTATGCGC CTACATGTAT TCGGTGAAGG AAACGGAACT TCTGAAACCA 
CTAAACATGA TTCAGCAGTT CCTGGCTCCG TTGACCAGCG AAGAGCTTTC CAGCCAGGAA 
AATGCCAAGG AGCGACTAGC TCTCTCCTAT CAGATTATCA GAAAAATGCA ACACGATGTG 
CATCCTGCCC CAAGTACCAA GTCCCGCCTC ATTTCACATT CCCCGCTAGT CGAGCAGTTT 
CGGGAGGTGT GGCGAACAGT AGTCGATGCT GGTCACCTGC CCGTTCGCGC CGCTCAGTCG 
TTGGAGTCGC TTCTGCTAGC CGGTGGCGCG GCGTGGCTGT CCACGCAGTT GGTGGAGCAA 
CTGCTGGCCT GCAAGTACAC AAGGGATATG TCGCGAACCA TGGACGTTGT GTTTGCCGTA 
ATGCATCTCG ACATTGAGAA GACCACTGAG GCGTTGCTCC AGTACGTGGT GGCTCCACTG 
ATACTGCGAC GACAAGGTGA GGACATTAAT GAGCCCCAAT CCCTGGTACT GGCCCGCCTC 
TGTGTTTACT GTATTATTTC CTGCCTGGAG AGCCGCAAAG GAAATGGCAC TTCCGCTTTG 
ACGGCCATGA AGAAGCGATC GAGATCTCAC GATGACGAGG AACTGGCGGC CAATGCAGCC 
AAAGTGCGCA AGGTGATTGG CGATGGCAGC GACAACTCAA GCGACTTTAC GGACACTACG 
ACCGGAGCTG GCCTGGCAGC ATTATTGGGC TCCACTTCCA CATCTGAACT GCGGACTACG 
CCGCTTACTC TAAGAGAACC ACTGCAGACG AGCGTGCAGC ACATTTTCGC AGTGTTCCTG 
CAGTTTGTCA GTGGAGATGA ACTGTCGCCC AAGGCCGTTT TTGTTTATCA GTTCATTTCG 
CTGCTGGTGG AATGCGGTGG TGAGAGGGTC GCTCCCGTCC TGCGCTTGCT GCCCAACGGC 
CTGGTGCAAC AGCTCCTGAA AGTTTTGGTC ACCGACGACA TAAAAGTGGG TCTCATCAGC 
AGACTATACG ATCTGCGAAT CCAGGCAGGA AGACTGTCCG CTGTGTCTGA TCTTTGCCTG 
TGGCGAAATA TGCAGATGGC CAGGCACAGC ATTCATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648185.1
CDS	120..3101
Translation	MKENNKILQLIYVAWRERWTDSQWGINIKKVLPRGVSGDVYNLADCLMQQALIGSTANPLVLNYLKHSLCAHLVSHAAVIRCIAKYDKLERVYCITALLEFLASIVDGVTCRIKSEEAVLPSSVVHLVYWLLQIFARTVQHYELYGEISAEQSYMLDQTCVVIDRLSQQQFLLSMLYVGCHEELEICGRIRDKYATIKGSLTNSNFTLNAPQVEQQLQQLAFIEAKHLEMQPLNPPSTLEKISCCVQPLLAVEVLLNPCKDTSYYVAELQMLQRLKKYSNTRLFYEIIRAGFLTLSNVADTSPDTMWGAFMFFKMPHIIKQLHALQRIPGEQPPPADYIPELVEALELLIEDNLLLDFMDTKCSCNMIEFLLNDWTKQQLVNDVHVKKFASQREAASQLLKKCDNGQQTPSNINFIKRAEVPLSGVLKTLCTNKVQDMVNVLCQVPVGNSFELILSVATVEGRLKTFVSRLIQCNENSKPVPGDLGKLCVIRSTLFDVSFLMLTSIVQTYGSDVVLSERGDSFFEKWVRECMVERNKLKNPRQILALCDDSIVDELLLSFSKPEASQLKPNNLSWQETCLNLPGVLHHVLIAWEQETLSSADVKSILDNIKRRLFSFSVCATSFLCAYMYSVKETELLKPLNMIQQFLAPLTSEELSSQENAKERLALSYQIIRKMQHDVHPAPSTKSRLISHSPLVEQFREVWRTVVDAGHLPVRAAQSLESLLLAGGAAWLSTQLVEQLLACKYTRDMSRTMDVVFAVMHLDIEKTTEALLQYVVAPLILRRQGEDINEPQSLVLARLCVYCIISCLESRKGNGTSALTAMKKRSRSHDDEELAANAAKVRKVIGDGSDNSSDFTDTTTGAGLAALLGSTSTSELRTTPLTLREPLQTSVQHIFAVFLQFVSGDELSPKAVFVYQFISLLVECGGERVAPVLRLLPNGLVQQLLKVLVTDDIKVGLISRLYDLRIQAGRLSAVSDLCLWRNMQMARHSIHL

Target ORF information:

RefSeq Version	NM_139928.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 24 (MED24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139928.5

ORF Insert Sequence:

ATGAAGGAGA ACAACAAGAT CCTCCAACTC ATCTATGTGG CGTGGCGCGA GCGCTGGACG 
GACTCCCAGT GGGGCATCAA CATAAAAAAG GTGCTGCCGA GAGGAGTCAG TGGAGATGTA 
TACAATCTGG CTGACTGTCT GATGCAGCAG GCACTCATTG GTTCCACGGC TAATCCGTTG 
GTTCTTAACT ACCTGAAGCA TTCACTGTGC GCCCATCTCG TTTCTCACGC TGCTGTTATT 
CGGTGCATTG CCAAATATGA CAAATTAGAG CGCGTTTATT GCATTACAGC GCTCCTTGAA 
TTCCTGGCCA GCATCGTGGA TGGCGTTACC TGCCGAATAA AATCTGAGGA AGCGGTTTTA 
CCGAGTTCCG TCGTCCACTT GGTGTACTGG CTGCTTCAAA TCTTCGCCAG AACTGTGCAG 
CACTATGAAC TGTATGGTGA GATTAGTGCT GAGCAATCAT ACATGCTGGA CCAGACCTGC 
GTGGTGATAG ATCGGCTTAG CCAGCAGCAG TTCCTGCTGT CTATGCTGTA CGTGGGCTGT 
CACGAAGAGC TCGAAATATG TGGACGGATT CGGGATAAGT ACGCTACCAT CAAGGGATCG 
TTGACGAACT CAAACTTTAC GCTAAATGCA CCACAAGTGG AGCAGCAATT GCAGCAGTTG 
GCCTTTATAG AAGCCAAGCA TCTTGAAATG CAACCACTTA ATCCGCCGTC AACACTCGAG 
AAGATCTCGT GCTGCGTGCA ACCCTTACTG GCGGTGGAAG TGCTTCTCAA TCCGTGCAAG 
GACACCAGCT ACTACGTGGC TGAGTTGCAG ATGCTGCAAC GATTAAAGAA ATACTCCAAT 
ACACGGCTCT TCTACGAAAT CATCCGTGCT GGATTCCTCA CGCTGAGCAA TGTTGCGGAT 
ACAAGTCCTG ACACTATGTG GGGTGCCTTT ATGTTCTTTA AAATGCCGCA CATCATTAAG 
CAATTGCACG CCCTGCAACG GATTCCCGGG GAGCAACCAC CACCGGCCGA TTACATACCC 
GAGTTGGTGG AGGCTTTGGA ATTGCTCATC GAGGACAATC TGCTGCTAGA CTTCATGGAT 
ACTAAGTGCT CATGCAATAT GATCGAGTTC CTTTTGAATG ACTGGACCAA GCAACAACTA 
GTCAATGATG TCCACGTAAA GAAGTTCGCC AGTCAAAGGG AAGCCGCCTC TCAATTACTC 
AAAAAATGCG ACAATGGCCA ACAGACACCC TCCAATATAA ACTTCATCAA GCGCGCAGAG 
GTTCCGCTTT CCGGAGTACT AAAAACGCTG TGCACCAACA AGGTCCAGGA CATGGTTAAT 
GTCCTTTGCC AAGTGCCGGT GGGCAACAGC TTCGAGTTGA TTCTTTCAGT GGCTACCGTC 
GAGGGACGCC TCAAGACATT CGTATCGCGT CTGATCCAGT GCAATGAGAA CTCCAAGCCG 
GTGCCGGGCG ACCTGGGCAA GCTATGCGTC ATCCGGTCCA CTCTGTTTGA TGTTTCCTTC 
CTAATGTTGA CAAGCATTGT GCAGACCTAT GGATCCGATG TGGTCTTATC TGAGCGTGGT 
GACTCATTTT TTGAAAAATG GGTGCGTGAG TGCATGGTGG AAAGGAATAA GCTTAAGAAT 
CCGCGTCAAA TTCTTGCCCT CTGTGATGAC AGTATTGTCG ACGAGCTTTT GCTTAGCTTC 
AGTAAACCAG AGGCATCACA ACTTAAGCCA AACAACTTAA GTTGGCAGGA GACTTGCCTT 
AATCTGCCCG GAGTGCTTCA TCATGTGCTA ATAGCTTGGG AGCAGGAAAC GCTATCCTCG 
GCGGACGTTA AAAGTATTCT GGACAATATT AAGAGAAGAC TTTTCAGTTT TTCCGTTTGC 
GCCACTAGTT TTCTATGCGC CTACATGTAT TCGGTGAAGG AAACGGAACT TCTGAAACCA 
CTAAACATGA TTCAGCAGTT CCTGGCTCCG TTGACCAGCG AAGAGCTTTC CAGCCAGGAA 
AATGCCAAGG AGCGACTAGC TCTCTCCTAT CAGATTATCA GAAAAATGCA ACACGATGTG 
CATCCTGCCC CAAGTACCAA GTCCCGCCTC ATTTCACATT CCCCGCTAGT CGAGCAGTTT 
CGGGAGGTGT GGCGAACAGT AGTCGATGCT GGTCACCTGC CCGTTCGCGC CGCTCAGTCG 
TTGGAGTCGC TTCTGCTAGC CGGTGGCGCG GCGTGGCTGT CCACGCAGTT GGTGGAGCAA 
CTGCTGGCCT GCAAGTACAC AAGGGATATG TCGCGAACCA TGGACGTTGT GTTTGCCGTA 
ATGCATCTCG ACATTGAGAA GACCACTGAG GCGTTGCTCC AGTACGTGGT GGCTCCACTG 
ATACTGCGAC GACAAGGTGA GGACATTAAT GAGCCCCAAT CCCTGGTACT GGCCCGCCTC 
TGTGTTTACT GTATTATTTC CTGCCTGGAG AGCCGCAAAG GAAATGGCAC TTCCGCTTTG 
ACGGCCATGA AGAAGCGATC GAGATCTCAC GATGACGAGG AACTGGCGGC CAATGCAGCC 
AAAGTGCGCA AGGTGATTGG CGATGGCAGC GACAACTCAA GCGACTTTAC GGACACTACG 
ACCGGAGCTG GCCTGGCAGC ATTATTGGGC TCCACTTCCA CATCTGAACT GCGGACTACG 
CCGCTTACTC TAAGAGAACC ACTGCAGACG AGCGTGCAGC ACATTTTCGC AGTGTTCCTG 
CAGTTTGTCA GTGGAGATGA ACTGTCGCCC AAGGCCGTTT TTGTTTATCA GTTCATTTCG 
CTGCTGGTGG AATGCGGTGG TGAGAGGGTC GCTCCCGTCC TGCGCTTGCT GCCCAACGGC 
CTGGTGCAAC AGCTCCTGAA AGTTTTGGTC ACCGACGACA TAAAAGTGGG TCTCATCAGC 
AGACTATACG ATCTGCGAAT CCAGGCAGGA AGACTGTCCG CTGTGTCTGA TCTTTGCCTG 
TGGCGAAATA TGCAGATGGC CAGGCACAGC ATTCATCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MED24 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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