cpb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cpb1
Entrez Gene ID	322412
Full Name	carboxypeptidase B1 (tissue)
Synonyms	wu:fb60d07,wu:fb69b08
General protein information	Preferred Names carboxypeptidase B1 (tissue) Names carboxypeptidase B zte141
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328425.1	NP_001315354.1	carboxypeptidase B isoform 1 precursor
NM_001110021.2	NP_001103491.2	carboxypeptidase B isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CPB1	NP_001098820.1
Rattus norvegicus (Norway rat)	Cpb1	NP_036665.1
Pan troglodytes (chimpanzee)	CPB1	XP_516808.2
Macaca mulatta (Rhesus monkey)	CPB1	XP_001110220.1
Danio rerio (zebrafish)	cpb1	NP_001103491.1
Xenopus tropicalis (tropical clawed frog)	LOC100494117	XP_002937383.2
Mus musculus (house mouse)	Cpb1	NP_083982.1
Gallus gallus (chicken)	CPB1	NP_001264374.1
Homo sapiens (human)	CPB1	NP_001862.2
Canis lupus familiaris (dog)	CPB1	NP_001003005.1
Drosophila melanogaster (fruit fly)	CG4017	NP_609309.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential embryotoxicity of the organic pollutants in rural and urban air particles.
Mesquita SR, van Drooge BL, Oliveira E, Grimalt JO, Barata C, Vieira N, Guimarães L, Piña B
Environmental pollution (Barking, Essex : 1987)206535-42(2015 Nov)

Proteomic analysis of arsenic-exposed zebrafish (Danio rerio) identifies altered expression in proteins involved in fibrosis and lipid uptake in a gender-specific manner.
Carlson P, Smalley DM, Van Beneden RJ
Toxicological sciences : an official journal of the Society of Toxicology134(1)83-91(2013 Jul)

Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
Zheng W, Xu H, Lam SH, Luo H, Karuturi RK, Gong Z
PloS one8(1)e53562(2013)

An osteosarcoma zebrafish model implicates Mmp-19 and Ets-1 as well as reduced host immune response in angiogenesis and migration.
Mohseny AB, Xiao W, Carvalho R, Spaink HP, Hogendoorn PC, Cleton-Jansen AM
The Journal of pathology227(2)245-53(2012 Jun)

Carboxypeptidase A6 in zebrafish development and implications for VIth cranial nerve pathfinding.
Lyons PJ, Ma LH, Baker R, Fricker LD
PloS one5(9)e12967(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cpb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cpb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39714	NM_001328425.1 Latest version!	Danio rerio carboxypeptidase B1 (tissue) (cpb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa60581	NM_001110021.2 Latest version!	Danio rerio carboxypeptidase B1 (tissue) (cpb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cpb1 ( NM_001328425.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315354.1 Danio rerio carboxypeptidase B1 (tissue) (cpb1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa39714
Clone ID Related Accession (Same CDS sequence)	NM_001328425.1
Accession Version	NM_001328425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1499184000000
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase B isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL935306.6, BC067637.1 and EE688842.1. On Jun 16, 2016 this sequence version replaced XM_005162487.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: GDQH01000111.1, GFIL01007012.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476736, SAMEA4476754 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTAT TTTTGCTCTT GGGATTGGTG GCAGTGGCTC TCTGTGAGCC AATCACATTT 
GTGGGTGATA AGGTCCTCCA GCTGAAACCC ATAAATGATG AACATGTGAC CATCATCAGG 
GAACTGGGCG AGAAAATCAA GCTGGATTTC TGGAAGCCTC GCAGTGCGGA TCTTGTGAGC 
ATCGACATGA CTGTTGATAT CCACGTTCCT GCAGCTCAGC TCGCCATGGT CTCCACCATC 
CTACAGCAGA GCGACATGGA AGTCAAGGTG ATGATTGATA ATCTTCAGGA GGCTGTGAAG 
GGTCAGATGG ACAACAGGAG TCCAACAAAG GGTCACGACT ACACAAAGTA CAACAGCTGG 
GCAACGATCA ATGACTGGGC AATCTCCATC AGCTCTGCCA ACCCTGACCT GATCAGCAGG 
CAGTCGATCG GTAACACTTA TGAGGGAAGA ACCATGCACC TTCTGAAGAT TGGAAAGAAT 
ACAGGGTCTA ATAAGCCAGC AGTCTTCATG GACTGTGGTT TTCATGCCAG AGAATGGATC 
ACCCATGCTT TCTGCCAGTG GTTTGTTAAT GAGGCTGTGT CCACATATGG CAGTGATCCT 
GACATGACCA ACCTTCTGGA CAGAATGGAC TTCTTTGTTC TGCCTGTCTT CAACATTGAT 
GGCTATGAAT ACACCTGGAA CAGAGACAGA ATGTGGAGAA AGACACGATC CAAGAACAGC 
GGCAGCAGTT GTATCGGTAC TGACCCCAAC AGAAACTTCA ATGCTGGATG GTGCACCGTT 
GGAGCTTCCA GCAACCCTTG CAGTGACACC TATTGTGGAA GCAGTCCAGA ATCTGAGATT 
GAGTCCAAGA ATTTGGCCAA CTTCATCCGC ACCAACAAGT CCGTCATCAA GGCCTACCTG 
ACAGTCCACT CGTATTCCCA GCTGCTGCTC TTCCCCTATT CCTACAAATA TGACCTGGCT 
GCTCACCACT CAGAGCTGAT GAGTGTTTCT CAGGGAGCTA TTGCAGCTCT GCGCAGCCTG 
TATGGAACCA AATACACTAG CGGCCCTGGT GCTGCGACTA TCTACCCTGC TGCTGGTGGC 
TCTGATGACT GGGCTTATGA TCTGGGTGTG AAATACTCCT ACACCTTTGA GCTGCGTGAT 
GAAGGCCGTT ACGGTTTCCT GCTGCCCGAG TCCCAGATCA AGCCTACTTG TGAGGAGACC 
ATGCTGGCTG TCAAATACAT CGCCAACCAC GTCCTCAACA ACCTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315354.1
CDS	59..1309
Translation	MKLFLLLGLVAVALCEPITFVGDKVLQLKPINDEHVTIIRELGEKIKLDFWKPRSADLVSIDMTVDIHVPAAQLAMVSTILQQSDMEVKVMIDNLQEAVKGQMDNRSPTKGHDYTKYNSWATINDWAISISSANPDLISRQSIGNTYEGRTMHLLKIGKNTGSNKPAVFMDCGFHAREWITHAFCQWFVNEAVSTYGSDPDMTNLLDRMDFFVLPVFNIDGYEYTWNRDRMWRKTRSKNSGSSCIGTDPNRNFNAGWCTVGASSNPCSDTYCGSSPESEIESKNLANFIRTNKSVIKAYLTVHSYSQLLLFPYSYKYDLAAHHSELMSVSQGAIAALRSLYGTKYTSGPGAATIYPAAGGSDDWAYDLGVKYSYTFELRDEGRYGFLLPESQIKPTCEETMLAVKYIANHVLNNLY

Target ORF information:

RefSeq Version	NM_001328425.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase B1 (tissue) (cpb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328425.1

ORF Insert Sequence:

ATGAAGTTAT TTTTGCTCTT GGGATTGGTG GCAGTGGCTC TCTGTGAGCC AATCACATTT 
GTGGGTGATA AGGTCCTCCA GCTGAAACCC ATAAATGATG AACATGTGAC CATCATCAGG 
GAACTGGGCG AGAAAATCAA GCTGGATTTC TGGAAGCCTC GCAGTGCGGA TCTTGTGAGC 
ATCGACATGA CTGTTGATAT CCACGTTCCT GCAGCTCAGC TCGCCATGGT CTCCACCATC 
CTACAGCAGA GCGACATGGA AGTCAAGGTG ATGATTGATA ATCTTCAGGA GGCTGTGAAG 
GGTCAGATGG ACAACAGGAG TCCAACAAAG GGTCACGACT ACACAAAGTA CAACAGCTGG 
GCAACGATCA ATGACTGGGC AATCTCCATC AGCTCTGCCA ACCCTGACCT GATCAGCAGG 
CAGTCGATCG GTAACACTTA TGAGGGAAGA ACCATGCACC TTCTGAAGAT TGGAAAGAAT 
ACAGGGTCTA ATAAGCCAGC AGTCTTCATG GACTGTGGTT TTCATGCCAG AGAATGGATC 
ACCCATGCTT TCTGCCAGTG GTTTGTTAAT GAGGCTGTGT CCACATATGG CAGTGATCCT 
GACATGACCA ACCTTCTGGA CAGAATGGAC TTCTTTGTTC TGCCTGTCTT CAACATTGAT 
GGCTATGAAT ACACCTGGAA CAGAGACAGA ATGTGGAGAA AGACACGATC CAAGAACAGC 
GGCAGCAGTT GTATCGGTAC TGACCCCAAC AGAAACTTCA ATGCTGGATG GTGCACCGTT 
GGAGCTTCCA GCAACCCTTG CAGTGACACC TATTGTGGAA GCAGTCCAGA ATCTGAGATT 
GAGTCCAAGA ATTTGGCCAA CTTCATCCGC ACCAACAAGT CCGTCATCAA GGCCTACCTG 
ACAGTCCACT CGTATTCCCA GCTGCTGCTC TTCCCCTATT CCTACAAATA TGACCTGGCT 
GCTCACCACT CAGAGCTGAT GAGTGTTTCT CAGGGAGCTA TTGCAGCTCT GCGCAGCCTG 
TATGGAACCA AATACACTAG CGGCCCTGGT GCTGCGACTA TCTACCCTGC TGCTGGTGGC 
TCTGATGACT GGGCTTATGA TCTGGGTGTG AAATACTCCT ACACCTTTGA GCTGCGTGAT 
GAAGGCCGTT ACGGTTTCCT GCTGCCCGAG TCCCAGATCA AGCCTACTTG TGAGGAGACC 
ATGCTGGCTG TCAAATACAT CGCCAACCAC GTCCTCAACA ACCTGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpb1 ( NM_001110021.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103491.2 Danio rerio carboxypeptidase B1 (tissue) (cpb1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa60581
Clone ID Related Accession (Same CDS sequence)	NM_001110021.2
Accession Version	NM_001110021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504627200000
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL935306.6. On Jun 16, 2016 this sequence version replaced NM_001110021.1. Transcript Variant: This variant (2) uses an alternate splice site in its 5' UTR, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC154043.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505392, SAMEA4476728 [ECO:0000350] ##Evidence-Data-END##

ATGCACCTTC TGAAGATTGG AAAGAATACA GGGTCTAATA AGCCAGCAGT CTTCATGGAC 
TGTGGTTTTC ATGCCAGAGA ATGGATCACC CATGCTTTCT GCCAGTGGTT TGTTAATGAG 
GCTGTGTCCA CATATGGCAG TGATCCTGAC ATGACCAACC TTCTGGACAG AATGGACTTC 
TTTGTTCTGC CTGTCTTCAA CATTGATGGC TATGAATACA CCTGGAACAG AGACAGAATG 
TGGAGAAAGA CACGATCCAA GAACAGCGGC AGCAGTTGTA TCGGTACTGA CCCCAACAGA 
AACTTCAATG CTGGATGGTG CACCGTTGGA GCTTCCAGCA ACCCTTGCAG TGACACCTAT 
TGTGGAAGCA GTCCAGAATC TGAGATTGAG TCCAAGAATT TGGCCAACTT CATCCGCACC 
AACAAGTCCG TCATCAAGGC CTACCTGACA GTCCACTCGT ATTCCCAGCT GCTGCTCTTC 
CCCTATTCCT ACAAATATGA CCTGGCTGCT CACCACTCAG AGCTGATGAG TGTTTCTCAG 
GGAGCTATTG CAGCTCTGCG CAGCCTGTAT GGAACCAAAT ACACTAGCGG CCCTGGTGCT 
GCGACTATCT ACCCTGCTGC TGGTGGCTCT GATGACTGGG CTTATGATCT GGGTGTGAAA 
TACTCCTACA CCTTTGAGCT GCGTGATGAA GGCCGTTACG GTTTCCTGCT GCCCGAGTCC 
CAGATCAAGC CTACTTGTGA GGAGACCATG CTGGCTGTCA AATACATCGC CAACCACGTC 
CTCAACAACC TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103491.2
CDS	447..1244
Translation	MHLLKIGKNTGSNKPAVFMDCGFHAREWITHAFCQWFVNEAVSTYGSDPDMTNLLDRMDFFVLPVFNIDGYEYTWNRDRMWRKTRSKNSGSSCIGTDPNRNFNAGWCTVGASSNPCSDTYCGSSPESEIESKNLANFIRTNKSVIKAYLTVHSYSQLLLFPYSYKYDLAAHHSELMSVSQGAIAALRSLYGTKYTSGPGAATIYPAAGGSDDWAYDLGVKYSYTFELRDEGRYGFLLPESQIKPTCEETMLAVKYIANHVLNNLY

Target ORF information:

RefSeq Version	NM_001110021.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase B1 (tissue) (cpb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110021.2

ORF Insert Sequence:

ATGCACCTTC TGAAGATTGG AAAGAATACA GGGTCTAATA AGCCAGCAGT CTTCATGGAC 
TGTGGTTTTC ATGCCAGAGA ATGGATCACC CATGCTTTCT GCCAGTGGTT TGTTAATGAG 
GCTGTGTCCA CATATGGCAG TGATCCTGAC ATGACCAACC TTCTGGACAG AATGGACTTC 
TTTGTTCTGC CTGTCTTCAA CATTGATGGC TATGAATACA CCTGGAACAG AGACAGAATG 
TGGAGAAAGA CACGATCCAA GAACAGCGGC AGCAGTTGTA TCGGTACTGA CCCCAACAGA 
AACTTCAATG CTGGATGGTG CACCGTTGGA GCTTCCAGCA ACCCTTGCAG TGACACCTAT 
TGTGGAAGCA GTCCAGAATC TGAGATTGAG TCCAAGAATT TGGCCAACTT CATCCGCACC 
AACAAGTCCG TCATCAAGGC CTACCTGACA GTCCACTCGT ATTCCCAGCT GCTGCTCTTC 
CCCTATTCCT ACAAATATGA CCTGGCTGCT CACCACTCAG AGCTGATGAG TGTTTCTCAG 
GGAGCTATTG CAGCTCTGCG CAGCCTGTAT GGAACCAAAT ACACTAGCGG CCCTGGTGCT 
GCGACTATCT ACCCTGCTGC TGGTGGCTCT GATGACTGGG CTTATGATCT GGGTGTGAAA 
TACTCCTACA CCTTTGAGCT GCGTGATGAA GGCCGTTACG GTTTCCTGCT GCCCGAGTCC 
CAGATCAAGC CTACTTGTGA GGAGACCATG CTGGCTGTCA AATACATCGC CAACCACGTC 
CTCAACAACC TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential embryotoxicity of the organic pollutants in rural and urban air particles.
Environmental pollution (Barking, Essex : 1987)206535-42(2015 Nov)
Mesquita SR,van Drooge BL,Oliveira E,Grimalt JO,Barata C,Vieira N,Guimarães L,Piña B

Proteomic analysis of arsenic-exposed zebrafish (Danio rerio) identifies altered expression in proteins involved in fibrosis and lipid uptake in a gender-specific manner.
Toxicological sciences : an official journal of the Society of Toxicology134(1)83-91(2013 Jul)
Carlson P,Smalley DM,Van Beneden RJ

Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.
PloS one8(1)e53562(2013)
Zheng W,Xu H,Lam SH,Luo H,Karuturi RK,Gong Z

An osteosarcoma zebrafish model implicates Mmp-19 and Ets-1 as well as reduced host immune response in angiogenesis and migration.
The Journal of pathology227(2)245-53(2012 Jun)
Mohseny AB,Xiao W,Carvalho R,Spaink HP,Hogendoorn PC,Cleton-Jansen AM

Carboxypeptidase A6 in zebrafish development and implications for VIth cranial nerve pathfinding.
PloS one5(9)e12967(2010 Sep)
Lyons PJ,Ma LH,Baker R,Fricker LD