psmd12 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	psmd12
Entrez Gene ID	321898
Full Name	proteasome 26S subunit, non-ATPase 12
Synonyms	wu:fb38a06,wu:fc34f08,wu:fc49f02
General protein information	Preferred Names proteasome 26S subunit, non-ATPase 12 Names 26S proteasome non-ATPase regulatory subunit 12 fc34f08 proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201578.1	NP_963872.1	26S proteasome non-ATPase regulatory subunit 12

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_02608	XP_003721188.1
Macaca mulatta (Rhesus monkey)	PSMD12	XP_001116817.1
Bos taurus (cattle)	PSMD12	NP_001039893.1
Oryza sativa (rice)	Os03g0851300	NP_001051914.1
Gallus gallus (chicken)	LOC100859465	XP_003642387.1
Homo sapiens (human)	PSMD12	NP_002807.1
Mus musculus (house mouse)	Psmd12	NP_080170.1
Gallus gallus (chicken)	PSMD12	NP_001025877.1
Danio rerio (zebrafish)	psmd12	NP_963872.1
Arabidopsis thaliana (thale cress)	RPN5B	NP_001032142.1
Schizosaccharomyces pombe (fission yeast)	rpn502	NP_594776.1
Kluyveromyces lactis	KLLA0C16313g	XP_452929.1
Neurospora crassa	NCU02650	XP_965423.1
Schizosaccharomyces pombe (fission yeast)	rpn501	NP_593278.1
Drosophila melanogaster (fruit fly)	Rpn5	NP_649588.1
Arabidopsis thaliana (thale cress)	EMB2107	NP_974758.1
Pan troglodytes (chimpanzee)	PSMD12	XP_511639.2
Canis lupus familiaris (dog)	PSMD12	XP_537584.2
Rattus norvegicus (Norway rat)	Psmd12	NP_001005875.1
Eremothecium gossypii	AGOS_ADR073W	NP_984169.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005535	XP_556176.3
Caenorhabditis elegans (roundworm)	rpn-5	NP_494835.1
Saccharomyces cerevisiae (baker's yeast)	RPN5	NP_010134.1

Gene Ontology
Bibliography

Related articles in PubMed

A combined FSTRA-shotgun proteomics approach to identify molecular changes in zebrafish upon chemical exposure.
Ayobahan SU, Eilebrecht E, Kotthoff M, Baumann L, Eilebrecht S, Teigeler M, Hollert H, Kalkhof S, Schäfers C
Scientific reports9(1)6599(2019 Apr)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

De Novo Disruption of the Proteasome Regulatory Subunit PSMD12 Causes a Syndromic Neurodevelopmental Disorder.
Küry S, Besnard T, Ebstein F, Khan TN, Gambin T, Douglas J, Bacino CA, Craigen WJ, Sanders SJ, Lehmann A, Latypova X, Khan K, Pacault M, Sacharow S, Glaser K, Bieth E, Perrin-Sabourin L, Jacquemont ML, Cho MT, Roeder E, Denommé-Pichon AS, Monaghan KG, Yuan B, Xia F, Simon S, Bonneau D, Parent P, Gilbert-Dussardier B, Odent S, Toutain A, Pasquier L, Barbouth D, Shaw CA, Patel A, Smith JL, Bi W, Schmitt S, Deb W, Nizon M, Mercier S, Vincent M, Rooryck C, Malan V, Briceño I, Gómez A, Nugent KM, Gibson JB, Cogné B, Lupski JR, Stessman HAF, Eichler EE, Retterer K, Yang Y, Redon R, Katsanis N, Rosenfeld JA, Kloetzel PM, Golzio C, Bézieau S, Stankiewicz P, Isidor B
American journal of human genetics100(2)352-363(2017 Feb)

Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish.
Dai Y, Prithiviraj N, Gan J, Zhang XA, Yan J
Scientific reports638693(2016 12)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following psmd12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the psmd12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34153	NM_201578.1 Latest version!	Danio rerio proteasome 26S subunit, non-ATPase 12 (psmd12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

psmd12 ( NM_201578.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_963872.1 Danio rerio proteasome 26S subunit, non-ATPase 12 (psmd12), mRNA.

Price & Availability↑

CloneID	ODa34153
Clone ID Related Accession (Same CDS sequence)	NM_201578.1
Accession Version	NM_201578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Danio rerio(zebrafish)
Product	26S proteasome non-ATPase regulatory subunit 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC042325.1. ##Evidence-Data-START## Transcript exon combination :: BC042325.1, BC165732.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505376, SAMEA4476757 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGATG ACAGAGCTGA ACGATCAGAT GGACGGATAG TGAAGATGGA GGTTGACTAC 
AGTGCCACTG TAGACGAGCG CCTTCCCGAG TGTGAGAAAA TGGCCAAGGA TGGCAGACTA 
CAGGAGGCAG TCGAGAGTTT GCTGTCACTA GAGAAGCAAA CAAGAACTGC TTCGGATATG 
GTGTCTACCT CCAGGATCTT GGTGGCAATA GTTCAGTTAT GCTATGAAGC CAAAGACTGG 
GATGCTCTGA ACGAAAACAT CATGCTGCTA TCTAAGAGAA GAAGCCAGCT GAAGCAGGCT 
GTTGCAAAGA TGGTACAGGA ATGTTACAAG TATGTTGATG CGATGACTGA CTTGTCAATC 
AAACTACGTC TCATCGACAC ACTTCGCACA GTCACTGCTG GAAAGATATA TGTGGAGATT 
GAACGTGCCA GACTGACTAA AACACTGGCC CATATCAAAG AGCAAAGTGG AGATGTGAAA 
GAAGCCGCAG CCATTCTGCA GGAGTTGCAG GTGGAGACAT ACGGATCAAT GGAGAAGAAA 
GAGAAGGCTG AGTTCATTCT GGAGCAGATG AGGCTGTGTA TTGCTGTCAA GGACTACATA 
CGCACACAGA TCATTAGCAA AAAAATCAAC ACGAAGTTCT TCCAGGAGGA AGGCACTGAG 
GAATTGAAAT TGAAGTATTA TAATCTGATG ATTCAAGTTG ATCTGCATGA GGGCTCCTAC 
CTGTCCATAT GTAAACACTA CAGAGCTATA TATGACACTC CTTGTATTCT GGAAGACTCG 
TCCAAATGGC AGCAGGCTCT GAAGAGTGTT GTGCTCTATG TGATTTTGGC GCCGTATGAC 
AATGAACAGT CTGATTTGGT TCATAGAATC AGCACTGACA AGAAACTGGA GGAAATACCC 
AAATACAGGG ATCTCCTTAA ACAGTTCACC ACCATGGAGC TGATGCGCTG GTCATCTGTA 
GTGGAGGATT ATGGCAAGGA ACTGAGGGAG GGTTCCATGG GAACTCCAGA CACTGATGTC 
TTCACCTGCA CAGAAGAGGG GGAGAAGAGA TGGAAAGATC TGAAAAATAG AGTGGTAGAA 
CATAACATCA GAATTATGGC AAAGTATTAC ACAAGCATCA CCATGGGGAG AATGGCAGCA 
CTGCTCGACC TCTCTGTTGA TGAATCAGAG GAGTTCTTAT CAAACCTGGT TGTCAACAAG 
ACCATCTATG CGAAAGTGGA CCGCCTCGCT GGCATCATAA ATTTCCAGCG GCCGAAAGAC 
CCTAACGATC TTCTGAATGA CTGGTCTCAA AAATTGAACT CTCTCATGTC TCTGGTCAAC 
AAAACCACTC ATCTCATTGC CAAAGAAGAG ATGATCCACA ACCTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963872.1
CDS	90..1460
Translation	MSDDRAERSDGRIVKMEVDYSATVDERLPECEKMAKDGRLQEAVESLLSLEKQTRTASDMVSTSRILVAIVQLCYEAKDWDALNENIMLLSKRRSQLKQAVAKMVQECYKYVDAMTDLSIKLRLIDTLRTVTAGKIYVEIERARLTKTLAHIKEQSGDVKEAAAILQELQVETYGSMEKKEKAEFILEQMRLCIAVKDYIRTQIISKKINTKFFQEEGTEELKLKYYNLMIQVDLHEGSYLSICKHYRAIYDTPCILEDSSKWQQALKSVVLYVILAPYDNEQSDLVHRISTDKKLEEIPKYRDLLKQFTTMELMRWSSVVEDYGKELREGSMGTPDTDVFTCTEEGEKRWKDLKNRVVEHNIRIMAKYYTSITMGRMAALLDLSVDESEEFLSNLVVNKTIYAKVDRLAGIINFQRPKDPNDLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ

Target ORF information:

RefSeq Version	NM_201578.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proteasome 26S subunit, non-ATPase 12 (psmd12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201578.1

ORF Insert Sequence:

ATGTCTGATG ACAGAGCTGA ACGATCAGAT GGACGGATAG TGAAGATGGA GGTTGACTAC 
AGTGCCACTG TAGACGAGCG CCTTCCCGAG TGTGAGAAAA TGGCCAAGGA TGGCAGACTA 
CAGGAGGCAG TCGAGAGTTT GCTGTCACTA GAGAAGCAAA CAAGAACTGC TTCGGATATG 
GTGTCTACCT CCAGGATCTT GGTGGCAATA GTTCAGTTAT GCTATGAAGC CAAAGACTGG 
GATGCTCTGA ACGAAAACAT CATGCTGCTA TCTAAGAGAA GAAGCCAGCT GAAGCAGGCT 
GTTGCAAAGA TGGTACAGGA ATGTTACAAG TATGTTGATG CGATGACTGA CTTGTCAATC 
AAACTACGTC TCATCGACAC ACTTCGCACA GTCACTGCTG GAAAGATATA TGTGGAGATT 
GAACGTGCCA GACTGACTAA AACACTGGCC CATATCAAAG AGCAAAGTGG AGATGTGAAA 
GAAGCCGCAG CCATTCTGCA GGAGTTGCAG GTGGAGACAT ACGGATCAAT GGAGAAGAAA 
GAGAAGGCTG AGTTCATTCT GGAGCAGATG AGGCTGTGTA TTGCTGTCAA GGACTACATA 
CGCACACAGA TCATTAGCAA AAAAATCAAC ACGAAGTTCT TCCAGGAGGA AGGCACTGAG 
GAATTGAAAT TGAAGTATTA TAATCTGATG ATTCAAGTTG ATCTGCATGA GGGCTCCTAC 
CTGTCCATAT GTAAACACTA CAGAGCTATA TATGACACTC CTTGTATTCT GGAAGACTCG 
TCCAAATGGC AGCAGGCTCT GAAGAGTGTT GTGCTCTATG TGATTTTGGC GCCGTATGAC 
AATGAACAGT CTGATTTGGT TCATAGAATC AGCACTGACA AGAAACTGGA GGAAATACCC 
AAATACAGGG ATCTCCTTAA ACAGTTCACC ACCATGGAGC TGATGCGCTG GTCATCTGTA 
GTGGAGGATT ATGGCAAGGA ACTGAGGGAG GGTTCCATGG GAACTCCAGA CACTGATGTC 
TTCACCTGCA CAGAAGAGGG GGAGAAGAGA TGGAAAGATC TGAAAAATAG AGTGGTAGAA 
CATAACATCA GAATTATGGC AAAGTATTAC ACAAGCATCA CCATGGGGAG AATGGCAGCA 
CTGCTCGACC TCTCTGTTGA TGAATCAGAG GAGTTCTTAT CAAACCTGGT TGTCAACAAG 
ACCATCTATG CGAAAGTGGA CCGCCTCGCT GGCATCATAA ATTTCCAGCG GCCGAAAGAC 
CCTAACGATC TTCTGAATGA CTGGTCTCAA AAATTGAACT CTCTCATGTC TCTGGTCAAC 
AAAACCACTC ATCTCATTGC CAAAGAAGAG ATGATCCACA ACCTCCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A combined FSTRA-shotgun proteomics approach to identify molecular changes in zebrafish upon chemical exposure.
Scientific reports9(1)6599(2019 Apr)
Ayobahan SU,Eilebrecht E,Kotthoff M,Baumann L,Eilebrecht S,Teigeler M,Hollert H,Kalkhof S,Schäfers C

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

De Novo Disruption of the Proteasome Regulatory Subunit PSMD12 Causes a Syndromic Neurodevelopmental Disorder.
American journal of human genetics100(2)352-363(2017 Feb)
Küry S,Besnard T,Ebstein F,Khan TN,Gambin T,Douglas J,Bacino CA,Craigen WJ,Sanders SJ,Lehmann A,Latypova X,Khan K,Pacault M,Sacharow S,Glaser K,Bieth E,Perrin-Sabourin L,Jacquemont ML,Cho MT,Roeder E,Denommé-Pichon AS,Monaghan KG,Yuan B,Xia F,Simon S,Bonneau D,Parent P,Gilbert-Dussardier B,Odent S,Toutain A,Pasquier L,Barbouth D,Shaw CA,Patel A,Smith JL,Bi W,Schmitt S,Deb W,Nizon M,Mercier S,Vincent M,Rooryck C,Malan V,Briceño I,Gómez A,Nugent KM,Gibson JB,Cogné B,Lupski JR,Stessman HAF,Eichler EE,Retterer K,Yang Y,Redon R,Katsanis N,Rosenfeld JA,Kloetzel PM,Golzio C,Bézieau S,Stankiewicz P,Isidor B

Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish.
Scientific reports638693(2016 12)
Dai Y,Prithiviraj N,Gan J,Zhang XA,Yan J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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psmd12 cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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