RRM1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RRM1
Entrez Gene ID	30740
Full Name	ribonucleotide reductase M1 polypeptide
Synonyms	CHUNP6866,cb396,cb838,r1,sb:cb548,wu:fb39b07,wu:fi14b02,wu:fk95f07
General protein information	Preferred Names ribonucleotide reductase M1 polypeptide Names ribonucleoside-diphosphate reductase large subunit ribonucleoside-diphosphate reductase subunit M1 ribonucleotide reductase large subunit ribonucleotide reductase protein r1 class I
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131455.2	NP_571530.2	ribonucleoside-diphosphate reductase large subunit
XM_017352747.1	XP_017208236.1	ribonucleoside-diphosphate reductase large subunit isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RRM1	XP_534027.2
Mus musculus (house mouse)	Rrm1	NP_033129.2
Gallus gallus (chicken)	RRM1	NP_001026008.1
Saccharomyces cerevisiae (baker's yeast)	RNR3	NP_012198.3
Eremothecium gossypii	AGOS_ADL057W	NP_984039.2
Schizosaccharomyces pombe (fission yeast)	cdc22	NP_594491.1
Pan troglodytes (chimpanzee)	RRM1	XP_001160029.1
Bos taurus (cattle)	RRM1	NP_001106767.1
Rattus norvegicus (Norway rat)	Rrm1	NP_001013254.1
Caenorhabditis elegans (roundworm)	rnr-1	NP_499039.1
Saccharomyces cerevisiae (baker's yeast)	RNR1	NP_010993.1
Oryza sativa (rice)	Os06g0168600	NP_001056926.1
Magnaporthe oryzae (rice blast fungus)	MGG_07000	XP_003709756.1
Danio rerio (zebrafish)	rrm1	NP_571530.1
Neurospora crassa	NCU03539	XP_956818.1
Xenopus tropicalis (tropical clawed frog)	rrm1	NP_001120566.1
Kluyveromyces lactis	KLLA0C07887g	XP_452551.1
Homo sapiens (human)	RRM1	NP_001024.1
Macaca mulatta (Rhesus monkey)	RRM1	XP_001113010.1
Drosophila melanogaster (fruit fly)	RnrL	NP_477027.1
Arabidopsis thaliana (thale cress)	RNR1	NP_179770.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010198	XP_554103.3

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The role of the DNA damage response in zebrafish and cellular models of Diamond Blackfan anemia.
Danilova N, Bibikova E, Covey TM, Nathanson D, Dimitrova E, Konto Y, Lindgren A, Glader B, Radu CG, Sakamoto KM, Lin S
Disease models & mechanisms7(7)895-905(2014 Jul)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuzé A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyriéras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

Regulation of E2F1 by the von Hippel-Lindau tumour suppressor protein predicts survival in renal cell cancer patients.
Mans DA, Vermaat JS, Weijts BG, van Rooijen E, van Reeuwijk J, Boldt K, Daenen LG, van der Groep P, Rowland BD, Jans JJ, Roepman R, Voest EE, van Diest PJ, Verhaar MC, de Bruin A, Giles RH
The Journal of pathology231(1)117-29(2013 Sep)

GLUT10 is required for the development of the cardiovascular system and the notochord and connects mitochondrial function to TGFβ signaling.
Willaert A, Khatri S, Callewaert BL, Coucke PJ, Crosby SD, Lee JG, Davis EC, Shiva S, Tsang M, De Paepe A, Urban Z
Human molecular genetics21(6)1248-59(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RRM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RRM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00741	NM_131455.2 Latest version!	Danio rerio ribonucleotide reductase M1 polypeptide (rrm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa56758	XM_017352747.1 Latest version!	Danio rerio ribonucleotide reductase M1 polypeptide (rrm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RRM1 ( NM_131455.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571530.2 Danio rerio ribonucleotide reductase M1 polypeptide (rrm1), mRNA.

Price & Availability↑

CloneID	ODa00741
Clone ID Related Accession (Same CDS sequence)	NM_131455.2
Accession Version	NM_131455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	ribonucleoside-diphosphate reductase large subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX511168.5, EH588550.1, EB847840.1, CT631855.2 and CT676109.2. On Dec 27, 2013 this sequence version replaced NM_131455.1. ##Evidence-Data-START## Transcript exon combination :: U57964.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCACGTGA TCAAGAGAGA TGGGCGCCAG GAGCGTGTAA TGTTTGACAA AATCACATCA 
CGCATCCAGA AACTTTGCTA TGGACTCAAC TCTGACTTTG TTGATCCAAC TCAGATCACC 
ATGAAGGTGA TTCAGGGCCT TTACAGCGGC GTTACTACAG TAGAGCTGGA CACACTGGCT 
GCTGAGATCG CTGCCACCCT CACCACCAAA CACCCTGACT ATGCCATCCT AGCAGCCCGC 
ATCGCCGTGT CTAACCTGCA TAAAGAGACT AAGAAGGTTT TCAGTGAGGT TATGGAGGAT 
CTCTACAACT ACGTCAATCC CCTGAACAGC CGCCACTCGC CTATGATCTC CAAGGAGACG 
CTTGATATTG TTTTGGCCAA CAAAGATCGC CTGAACTCAG CCATCATTTA TGACAGGGAT 
TTCTCTTACA ACTTCTTTGG CTTCAAGACA TTGGAGCGCT CATATCTGCT GAAGATCAAT 
GGAAAGGTTG CCGAGCGGCC GCAGCATATG CTGATGAGGG TGTCCGTAGG CATTCATAAG 
GAAGACATTG CGGCTGCTAT CGAAACCTAC AACCTGCTGT CTGAGAAGTG GTTCACTCAT 
GCTTCTCCTA CTCTGTTTAA TGCGGGCACC AACCGGCCAC AGCTCTCCAG TTGCTTCCTG 
CTTGCTATGA AAGATGACAG CATTGAGGGC ATCTATGACA CCCTGAAGCA GTGTGCGCTC 
ATCTCCAAGT CTGCCGGTGG CATCGGGGTG GCAGTGAGCT GCATTAGAGC CACTGGCAGC 
TACATTGCAG GAACAAATGG TAACTCAAAT GGCCTGGTTC CTATGCTCCG TGTCTACAAC 
AACACCGCAC GCTATGTGGA CCAAGGGGGA AATAAGAGAC CAGGAGCTTT TGCCATGTAC 
CTGGAGCCTT GGCATTTTGA CATTTTCGAC TTCCTAGAGC TTAAAAAGAA CACTGGTAAA 
GAGGAGCAGA GAGCCAGAGA TCTGTTTTAC GCCCTCTGGA TTCCTGATCT CTTCATGAAG 
CGAGTGGAGA CCAATGGGGA TTGGTCTCTG ATGTGCCCAA ATGACTGTCC CGGCCTGGAT 
GAGTGCTGGG GAGAGGAGTT TGAGAAGCTC TATGCAAAAT ATGAGCAGGA GGGCAGAGCG 
AAGCGTGTGG TTAAGGCTCA GCAGCTTTGG TATGCCATCA TCGAGTCTCA GACTGAGACC 
GGCACACCAT ACATGCTTTA CAAAGACGCC TGCAACCGCA AGAGCAACCA GCAGAACCTG 
GGCACCATCA AATGCAGCAA TTTGTGCACA GAGATTGTTG AGTACACCAG TGCCGATGAG 
GTGGCAGTTT GCAACCTGGC CTCCATTGCA CTTAACATGT ACGTCACCTC AGAGCGAACA 
TTCGACTTCC AGAAGTTGGC CTCTGTTACG AAGGTCATTG TGAAGAACCT GAACAAGATC 
ATTGATATCA ACTACTACCC AGTTAAAGAG GCTGAGAACT CCAACAAGCG CCACCGGCCC 
ATCGGGATCG GCGTTCAGGG TCTGGCCGAT GCCTTCATCC TCATGCGTTT CCCATTTGAG 
AGTGCGGAGG CTCAACTCCT CAACACGCAG ATCTTTGAGA CCATCTATTA CGCTGCTCTG 
GAGTCCAGCT GTGAGCTCGC TGCTGAATAC GGCCCGTATC AAACCTACGC CGGCTGTCCT 
GTCAGCAAGG GTATCTTGCA GTATGATATG TGGGAAAAGA CACCTACAGA CTTGTGGGAC 
TGGGCAGCGC TTAAGGAGAA GATTGCAAAG CATGGGGTGC GTAACAGTCT GCTGCTGGCC 
CCAATGCCCA CTGCCTCCAC AGCTCAGATT CTGGGCAACA ACGAATCCAT TGAGCCGTAC 
ACCAGCAACA TCTACACCCG CAGGGTCCTG TCTGGAGAGT TTCAGATCGT GAACCCGCAT 
CTTCTCAAAG ACCTCACAGA ACGAGGACTC TGGAACGAGG AAATGAAAAA CCAGATCATC 
GCTCAGAACG GATCCATCCA GACCATTCCC GCAATCCCTG ATGACCTGAA GGAGCTGTAT 
AAGACAGTGT GGGAGATTTC TCAGAAGACC ATTTTAAAGA TGGCTGCAGA TCGAGGAGCC 
TACATTGACC AGAGCCAGTC GCTCAACATC CACATCGCTG AGCCAAACTA CGGCAAACTC 
ACCAGCATGC ACTTCTACGG CTGGAAGCAG GGTCTTAAAA CAGGCATGTA CTACCTGAGA 
ACTAAACCGG CTGCAAACCC CATCCAGTTC ACCCTCAACA AAGAGAAGCT GAAGGAGACA 
CAGAAAACGA CCAGCAGTGA AGACGAGGAA ACCAAAGAGC GTAATAAAGC TGCGATGGTG 
TGTTCACTGG AGAACCGTGA CGAGTGTCTG ATGTGCGGAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571530.2
CDS	169..2553
Translation	MHVIKRDGRQERVMFDKITSRIQKLCYGLNSDFVDPTQITMKVIQGLYSGVTTVELDTLAAEIAATLTTKHPDYAILAARIAVSNLHKETKKVFSEVMEDLYNYVNPLNSRHSPMISKETLDIVLANKDRLNSAIIYDRDFSYNFFGFKTLERSYLLKINGKVAERPQHMLMRVSVGIHKEDIAAAIETYNLLSEKWFTHASPTLFNAGTNRPQLSSCFLLAMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPMLRVYNNTARYVDQGGNKRPGAFAMYLEPWHFDIFDFLELKKNTGKEEQRARDLFYALWIPDLFMKRVETNGDWSLMCPNDCPGLDECWGEEFEKLYAKYEQEGRAKRVVKAQQLWYAIIESQTETGTPYMLYKDACNRKSNQQNLGTIKCSNLCTEIVEYTSADEVAVCNLASIALNMYVTSERTFDFQKLASVTKVIVKNLNKIIDINYYPVKEAENSNKRHRPIGIGVQGLADAFILMRFPFESAEAQLLNTQIFETIYYAALESSCELAAEYGPYQTYAGCPVSKGILQYDMWEKTPTDLWDWAALKEKIAKHGVRNSLLLAPMPTASTAQILGNNESIEPYTSNIYTRRVLSGEFQIVNPHLLKDLTERGLWNEEMKNQIIAQNGSIQTIPAIPDDLKELYKTVWEISQKTILKMAADRGAYIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTKPAANPIQFTLNKEKLKETQKTTSSEDEETKERNKAAMVCSLENRDECLMCGS

Target ORF information:

RefSeq Version	NM_131455.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ribonucleotide reductase M1 polypeptide (rrm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131455.2

ORF Insert Sequence:

ATGCACGTGA TCAAGAGAGA TGGGCGCCAG GAGCGTGTAA TGTTTGACAA AATCACATCA 
CGCATCCAGA AACTTTGCTA TGGACTCAAC TCTGACTTTG TTGATCCAAC TCAGATCACC 
ATGAAGGTGA TTCAGGGCCT TTACAGCGGC GTTACTACAG TAGAGCTGGA CACACTGGCT 
GCTGAGATCG CTGCCACCCT CACCACCAAA CACCCTGACT ATGCCATCCT AGCAGCCCGC 
ATCGCCGTGT CTAACCTGCA TAAAGAGACT AAGAAGGTTT TCAGTGAGGT TATGGAGGAT 
CTCTACAACT ACGTCAATCC CCTGAACAGC CGCCACTCGC CTATGATCTC CAAGGAGACG 
CTTGATATTG TTTTGGCCAA CAAAGATCGC CTGAACTCAG CCATCATTTA TGACAGGGAT 
TTCTCTTACA ACTTCTTTGG CTTCAAGACA TTGGAGCGCT CATATCTGCT GAAGATCAAT 
GGAAAGGTTG CCGAGCGGCC GCAGCATATG CTGATGAGGG TGTCCGTAGG CATTCATAAG 
GAAGACATTG CGGCTGCTAT CGAAACCTAC AACCTGCTGT CTGAGAAGTG GTTCACTCAT 
GCTTCTCCTA CTCTGTTTAA TGCGGGCACC AACCGGCCAC AGCTCTCCAG TTGCTTCCTG 
CTTGCTATGA AAGATGACAG CATTGAGGGC ATCTATGACA CCCTGAAGCA GTGTGCGCTC 
ATCTCCAAGT CTGCCGGTGG CATCGGGGTG GCAGTGAGCT GCATTAGAGC CACTGGCAGC 
TACATTGCAG GAACAAATGG TAACTCAAAT GGCCTGGTTC CTATGCTCCG TGTCTACAAC 
AACACCGCAC GCTATGTGGA CCAAGGGGGA AATAAGAGAC CAGGAGCTTT TGCCATGTAC 
CTGGAGCCTT GGCATTTTGA CATTTTCGAC TTCCTAGAGC TTAAAAAGAA CACTGGTAAA 
GAGGAGCAGA GAGCCAGAGA TCTGTTTTAC GCCCTCTGGA TTCCTGATCT CTTCATGAAG 
CGAGTGGAGA CCAATGGGGA TTGGTCTCTG ATGTGCCCAA ATGACTGTCC CGGCCTGGAT 
GAGTGCTGGG GAGAGGAGTT TGAGAAGCTC TATGCAAAAT ATGAGCAGGA GGGCAGAGCG 
AAGCGTGTGG TTAAGGCTCA GCAGCTTTGG TATGCCATCA TCGAGTCTCA GACTGAGACC 
GGCACACCAT ACATGCTTTA CAAAGACGCC TGCAACCGCA AGAGCAACCA GCAGAACCTG 
GGCACCATCA AATGCAGCAA TTTGTGCACA GAGATTGTTG AGTACACCAG TGCCGATGAG 
GTGGCAGTTT GCAACCTGGC CTCCATTGCA CTTAACATGT ACGTCACCTC AGAGCGAACA 
TTCGACTTCC AGAAGTTGGC CTCTGTTACG AAGGTCATTG TGAAGAACCT GAACAAGATC 
ATTGATATCA ACTACTACCC AGTTAAAGAG GCTGAGAACT CCAACAAGCG CCACCGGCCC 
ATCGGGATCG GCGTTCAGGG TCTGGCCGAT GCCTTCATCC TCATGCGTTT CCCATTTGAG 
AGTGCGGAGG CTCAACTCCT CAACACGCAG ATCTTTGAGA CCATCTATTA CGCTGCTCTG 
GAGTCCAGCT GTGAGCTCGC TGCTGAATAC GGCCCGTATC AAACCTACGC CGGCTGTCCT 
GTCAGCAAGG GTATCTTGCA GTATGATATG TGGGAAAAGA CACCTACAGA CTTGTGGGAC 
TGGGCAGCGC TTAAGGAGAA GATTGCAAAG CATGGGGTGC GTAACAGTCT GCTGCTGGCC 
CCAATGCCCA CTGCCTCCAC AGCTCAGATT CTGGGCAACA ACGAATCCAT TGAGCCGTAC 
ACCAGCAACA TCTACACCCG CAGGGTCCTG TCTGGAGAGT TTCAGATCGT GAACCCGCAT 
CTTCTCAAAG ACCTCACAGA ACGAGGACTC TGGAACGAGG AAATGAAAAA CCAGATCATC 
GCTCAGAACG GATCCATCCA GACCATTCCC GCAATCCCTG ATGACCTGAA GGAGCTGTAT 
AAGACAGTGT GGGAGATTTC TCAGAAGACC ATTTTAAAGA TGGCTGCAGA TCGAGGAGCC 
TACATTGACC AGAGCCAGTC GCTCAACATC CACATCGCTG AGCCAAACTA CGGCAAACTC 
ACCAGCATGC ACTTCTACGG CTGGAAGCAG GGTCTTAAAA CAGGCATGTA CTACCTGAGA 
ACTAAACCGG CTGCAAACCC CATCCAGTTC ACCCTCAACA AAGAGAAGCT GAAGGAGACA 
CAGAAAACGA CCAGCAGTGA AGACGAGGAA ACCAAAGAGC GTAATAAAGC TGCGATGGTG 
TGTTCACTGG AGAACCGTGA CGAGTGTCTG ATGTGCGGAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRM1 ( XM_017352747.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208236.1 Danio rerio ribonucleotide reductase M1 polypeptide (rrm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56758
Clone ID Related Accession (Same CDS sequence)	XM_017352747.1
Accession Version	XM_017352747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ribonucleoside-diphosphate reductase large subunit isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTATTT TTTTTTTTTT CACAGATGGG CGCCAGGAGC GTGTAATGTT TGACAAAATC 
ACATCACGCA TCCAGAAACT TTGCTATGGA CTCAACTCTG ACTTTGTTGA TCCAACTCAG 
ATCACCATGA AGGTGATTCA GGGCCTTTAC AGCGGCGTTA CTACAGTAGA GCTGGACACA 
CTGGCTGCTG AGATCGCTGC CACCCTCACC ACCAAACACC CTGACTATGC CATCCTAGCA 
GCCCGCATCG CCGTGTCTAA CCTGCATAAA GAGACTAAGA AGGTTTTCAG TGAGGTTATG 
GAGGATCTCT ACAACTACGT CAATCCCCTG AACAGCCGCC ACTCGCCTAT GATCTCCAAG 
GAGACGCTTG ATATTGTTTT GGCCAACAAA GATCGCCTGA ACTCAGCCAT CATTTATGAC 
AGGGATTTCT CTTACAACTT CTTTGGCTTC AAGACATTGG AGCGCTCATA TCTGCTGAAG 
ATCAATGGAA AGGTTGCCGA GCGGCCGCAG CATATGCTGA TGAGGGTGTC CGTAGGCATT 
CATAAGGAAG ACATTGCGGC TGCTATCGAA ACCTACAACC TGCTGTCTGA GAAGTGGTTC 
ACTCATGCTT CTCCTACTCT GTTTAATGCG GGCACCAACC GGCCACAGCT CTCCAGTTGC 
TTCCTGCTTG CTATGAAAGA TGACAGCATT GAGGGCATCT ATGACACCCT GAAGCAGTGT 
GCGCTCATCT CCAAGTCTGC CGGTGGCATC GGGGTGGCAG TGAGCTGCAT TAGAGCCACT 
GGCAGCTACA TTGCAGGAAC AAATGGTAAC TCAAATGGCC TGGTTCCTAT GCTCCGTGTC 
TACAACAACA CCGCACGCTA TGTGGACCAA GGGGGAAATA AGAGACCAGG AGCTTTTGCC 
ATGTACCTGG AGCCTTGGCA TTTTGACATT TTCGACTTCC TAGAGCTTAA AAAGAACACT 
GGTAAAGAGG AGCAGAGAGC CAGAGATCTG TTTTACGCCC TCTGGATTCC TGATCTCTTC 
ATGAAGCGAG TGGAGACCAA TGGGGATTGG TCTCTGATGT GCCCAAATGA CTGTCCCGGC 
CTGGATGAGT GCTGGGGAGA GGAGTTTGAG AAGCTCTATG CAAAATATGA GCAGGAGGGC 
AGAGCGAAGC GTGTGGTTAA GGCTCAGCAG CTTTGGTATG CCATCATCGA GTCTCAGACT 
GAGACCGGCA CACCATACAT GCTTTACAAA GACGCCTGCA ACCGCAAGAG CAACCAGCAG 
AACCTGGGCA CCATCAAATG CAGCAATTTG TGCACAGAGA TTGTTGAGTA CACCAGTGCC 
GATGAGGTGG CAGTTTGCAA CCTGGCCTCC ATTGCACTTA ACATGTACGT CACCTCAGAG 
CGAACATTCG ACTTCCAGAA GTTGGCCTCT GTTACGAAGG TCATTGTGAA GAACCTGAAC 
AAGATCATTG ATATCAACTA CTACCCAGTT AAAGAGGCTG AGAACTCCAA CAAGCGCCAC 
CGGCCCATCG GGATCGGCGT TCAGGGTCTG GCCGATGCCT TCATCCTCAT GCGTTTCCCA 
TTTGAGAGTG CGGAGGCTCA ACTCCTCAAC ACGCAGATCT TTGAGACCAT CTATTACGCT 
GCTCTGGAGT CCAGCTGTGA GCTCGCTGCT GAATACGGCC CGTATCAAAC CTACGCCGGC 
TGTCCTGTCA GCAAGGGTAT CTTGCAGTAT GATATGTGGG AAAAGACACC TACAGACTTG 
TGGGACTGGG CAGCGCTTAA GGAGAAGATT GCAAAGCATG GGGTGCGTAA CAGTCTGCTG 
CTGGCCCCAA TGCCCACTGC CTCCACAGCT CAGATTCTGG GCAACAACGA ATCCATTGAG 
CCGTACACCA GCAACATCTA CACCCGCAGG GTCCTGTCTG GAGAGTTTCA GATCGTGAAC 
CCGCATCTTC TCAAAGACCT CACAGAACGA GGACTCTGGA ACGAGGAAAT GAAAAACCAG 
ATCATCGCTC AGAACGGATC CATCCAGACC ATTCCCGCAA TCCCTGATGA CCTGAAGGAG 
CTGTATAAGA CAGTGTGGGA GATTTCTCAG AAGACCATTT TAAAGATGGC TGCAGATCGA 
GGAGCCTACA TTGACCAGAG CCAGTCGCTC AACATCCACA TCGCTGAGCC AAACTACGGC 
AAACTCACCA GCATGCACTT CTACGGCTGG AAGCAGGGTC TTAAAACAGG CATGTACTAC 
CTGAGAACTA AACCGGCTGC AAACCCCATC CAGTTCACCC TCAACAAAGA GAAGCTGAAG 
GAGACACAGA AAACGACCAG CAGTGAAGAC GAGGAAACCA AAGAGCGTAA TAAAGCTGCG 
ATGGTGTGTT CACTGGAGAA CCGTGACGAG TGTCTGATGT GCGGATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208236.1
CDS	443..2833
Translation	MTIFFFFTDGRQERVMFDKITSRIQKLCYGLNSDFVDPTQITMKVIQGLYSGVTTVELDTLAAEIAATLTTKHPDYAILAARIAVSNLHKETKKVFSEVMEDLYNYVNPLNSRHSPMISKETLDIVLANKDRLNSAIIYDRDFSYNFFGFKTLERSYLLKINGKVAERPQHMLMRVSVGIHKEDIAAAIETYNLLSEKWFTHASPTLFNAGTNRPQLSSCFLLAMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPMLRVYNNTARYVDQGGNKRPGAFAMYLEPWHFDIFDFLELKKNTGKEEQRARDLFYALWIPDLFMKRVETNGDWSLMCPNDCPGLDECWGEEFEKLYAKYEQEGRAKRVVKAQQLWYAIIESQTETGTPYMLYKDACNRKSNQQNLGTIKCSNLCTEIVEYTSADEVAVCNLASIALNMYVTSERTFDFQKLASVTKVIVKNLNKIIDINYYPVKEAENSNKRHRPIGIGVQGLADAFILMRFPFESAEAQLLNTQIFETIYYAALESSCELAAEYGPYQTYAGCPVSKGILQYDMWEKTPTDLWDWAALKEKIAKHGVRNSLLLAPMPTASTAQILGNNESIEPYTSNIYTRRVLSGEFQIVNPHLLKDLTERGLWNEEMKNQIIAQNGSIQTIPAIPDDLKELYKTVWEISQKTILKMAADRGAYIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTKPAANPIQFTLNKEKLKETQKTTSSEDEETKERNKAAMVCSLENRDECLMCGS

Target ORF information:

RefSeq Version	XM_017352747.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ribonucleotide reductase M1 polypeptide (rrm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352747.1

ORF Insert Sequence:

ATGACTATTT TTTTTTTTTT CACAGATGGG CGCCAGGAGC GTGTAATGTT TGACAAAATC 
ACATCACGCA TCCAGAAACT TTGCTATGGA CTCAACTCTG ACTTTGTTGA TCCAACTCAG 
ATCACCATGA AGGTGATTCA GGGCCTTTAC AGCGGCGTTA CTACAGTAGA GCTGGACACA 
CTGGCTGCTG AGATCGCTGC CACCCTCACC ACCAAACACC CTGACTATGC CATCCTAGCA 
GCCCGCATCG CCGTGTCTAA CCTGCATAAA GAGACTAAGA AGGTTTTCAG TGAGGTTATG 
GAGGATCTCT ACAACTACGT CAATCCCCTG AACAGCCGCC ACTCGCCTAT GATCTCCAAG 
GAGACGCTTG ATATTGTTTT GGCCAACAAA GATCGCCTGA ACTCAGCCAT CATTTATGAC 
AGGGATTTCT CTTACAACTT CTTTGGCTTC AAGACATTGG AGCGCTCATA TCTGCTGAAG 
ATCAATGGAA AGGTTGCCGA GCGGCCGCAG CATATGCTGA TGAGGGTGTC CGTAGGCATT 
CATAAGGAAG ACATTGCGGC TGCTATCGAA ACCTACAACC TGCTGTCTGA GAAGTGGTTC 
ACTCATGCTT CTCCTACTCT GTTTAATGCG GGCACCAACC GGCCACAGCT CTCCAGTTGC 
TTCCTGCTTG CTATGAAAGA TGACAGCATT GAGGGCATCT ATGACACCCT GAAGCAGTGT 
GCGCTCATCT CCAAGTCTGC CGGTGGCATC GGGGTGGCAG TGAGCTGCAT TAGAGCCACT 
GGCAGCTACA TTGCAGGAAC AAATGGTAAC TCAAATGGCC TGGTTCCTAT GCTCCGTGTC 
TACAACAACA CCGCACGCTA TGTGGACCAA GGGGGAAATA AGAGACCAGG AGCTTTTGCC 
ATGTACCTGG AGCCTTGGCA TTTTGACATT TTCGACTTCC TAGAGCTTAA AAAGAACACT 
GGTAAAGAGG AGCAGAGAGC CAGAGATCTG TTTTACGCCC TCTGGATTCC TGATCTCTTC 
ATGAAGCGAG TGGAGACCAA TGGGGATTGG TCTCTGATGT GCCCAAATGA CTGTCCCGGC 
CTGGATGAGT GCTGGGGAGA GGAGTTTGAG AAGCTCTATG CAAAATATGA GCAGGAGGGC 
AGAGCGAAGC GTGTGGTTAA GGCTCAGCAG CTTTGGTATG CCATCATCGA GTCTCAGACT 
GAGACCGGCA CACCATACAT GCTTTACAAA GACGCCTGCA ACCGCAAGAG CAACCAGCAG 
AACCTGGGCA CCATCAAATG CAGCAATTTG TGCACAGAGA TTGTTGAGTA CACCAGTGCC 
GATGAGGTGG CAGTTTGCAA CCTGGCCTCC ATTGCACTTA ACATGTACGT CACCTCAGAG 
CGAACATTCG ACTTCCAGAA GTTGGCCTCT GTTACGAAGG TCATTGTGAA GAACCTGAAC 
AAGATCATTG ATATCAACTA CTACCCAGTT AAAGAGGCTG AGAACTCCAA CAAGCGCCAC 
CGGCCCATCG GGATCGGCGT TCAGGGTCTG GCCGATGCCT TCATCCTCAT GCGTTTCCCA 
TTTGAGAGTG CGGAGGCTCA ACTCCTCAAC ACGCAGATCT TTGAGACCAT CTATTACGCT 
GCTCTGGAGT CCAGCTGTGA GCTCGCTGCT GAATACGGCC CGTATCAAAC CTACGCCGGC 
TGTCCTGTCA GCAAGGGTAT CTTGCAGTAT GATATGTGGG AAAAGACACC TACAGACTTG 
TGGGACTGGG CAGCGCTTAA GGAGAAGATT GCAAAGCATG GGGTGCGTAA CAGTCTGCTG 
CTGGCCCCAA TGCCCACTGC CTCCACAGCT CAGATTCTGG GCAACAACGA ATCCATTGAG 
CCGTACACCA GCAACATCTA CACCCGCAGG GTCCTGTCTG GAGAGTTTCA GATCGTGAAC 
CCGCATCTTC TCAAAGACCT CACAGAACGA GGACTCTGGA ACGAGGAAAT GAAAAACCAG 
ATCATCGCTC AGAACGGATC CATCCAGACC ATTCCCGCAA TCCCTGATGA CCTGAAGGAG 
CTGTATAAGA CAGTGTGGGA GATTTCTCAG AAGACCATTT TAAAGATGGC TGCAGATCGA 
GGAGCCTACA TTGACCAGAG CCAGTCGCTC AACATCCACA TCGCTGAGCC AAACTACGGC 
AAACTCACCA GCATGCACTT CTACGGCTGG AAGCAGGGTC TTAAAACAGG CATGTACTAC 
CTGAGAACTA AACCGGCTGC AAACCCCATC CAGTTCACCC TCAACAAAGA GAAGCTGAAG 
GAGACACAGA AAACGACCAG CAGTGAAGAC GAGGAAACCA AAGAGCGTAA TAAAGCTGCG 
ATGGTGTGTT CACTGGAGAA CCGTGACGAG TGTCTGATGT GCGGATCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The role of the DNA damage response in zebrafish and cellular models of Diamond Blackfan anemia.
Disease models & mechanisms7(7)895-905(2014 Jul)
Danilova N,Bibikova E,Covey TM,Nathanson D,Dimitrova E,Konto Y,Lindgren A,Glader B,Radu CG,Sakamoto KM,Lin S

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuzé A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyriéras N,Jamen F,Joly JS

Regulation of E2F1 by the von Hippel-Lindau tumour suppressor protein predicts survival in renal cell cancer patients.
The Journal of pathology231(1)117-29(2013 Sep)
Mans DA,Vermaat JS,Weijts BG,van Rooijen E,van Reeuwijk J,Boldt K,Daenen LG,van der Groep P,Rowland BD,Jans JJ,Roepman R,Voest EE,van Diest PJ,Verhaar MC,de Bruin A,Giles RH

GLUT10 is required for the development of the cardiovascular system and the notochord and connects mitochondrial function to TGFβ signaling.
Human molecular genetics21(6)1248-59(2012 Mar)
Willaert A,Khatri S,Callewaert BL,Coucke PJ,Crosby SD,Lee JG,Davis EC,Shiva S,Tsang M,De Paepe A,Urban Z

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RRM1 cDNA ORF clone, Danio rerio(Zebrafish)

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