epha2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	epha2a
Entrez Gene ID	30689
Full Name	eph receptor A2 a
Synonyms	epha2,id:ibd5152,rtk6
General protein information	Preferred Names eph receptor A2 a Names ephrin type-A receptor 2 eph-like receptor tyrosine kinase 6
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131415.1	NP_571490.1	ephrin type-A receptor 2 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	epha2a	NP_571490.1
Danio rerio (zebrafish)	LOC568718	XP_005162266.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Comparative transcriptomic profiling of hydrogen peroxide signaling networks in zebrafish and human keratinocytes: Implications toward conservation, migration and wound healing.
Lisse TS, King BL, Rieger S
Scientific reports620328(2016 Feb)

Involvement of EphA2-mediated tyrosine phosphorylation of Shp2 in Shp2-regulated activation of extracellular signal-regulated kinase.
Miura K, Wakayama Y, Tanino M, Orba Y, Sawa H, Hatakeyama M, Tanaka S, Sabe H, Mochizuki N
Oncogene32(45)5292-301(2013 Nov)

Tardbpl splicing rescues motor neuron and axonal development in a mutant tardbp zebrafish.
Hewamadduma CA, Grierson AJ, Ma TP, Pan L, Moens CB, Ingham PW, Ramesh T, Shaw PJ
Human molecular genetics22(12)2376-86(2013 Jun)

Characterisation of five novel zebrafish Eph-related receptor tyrosine kinases suggests roles in patterning the neural plate.
Cooke JE, Xu Q, Wilson SW, Holder N
Development genes and evolution206(8)515-531(1997 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following epha2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the epha2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01024	NM_131415.1 Latest version!	Danio rerio eph receptor A2 a (epha2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

epha2a ( NM_131415.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571490.1 Danio rerio eph receptor A2 a (epha2a), mRNA.

Price & Availability↑

CloneID	ODa01024
Clone ID Related Accession (Same CDS sequence)	NM_131415.1
Accession Version	NM_131415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	ephrin type-A receptor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC065990.1. On Jul 6, 2005 this sequence version replaced XM_694966.1. ##Evidence-Data-START## Transcript exon combination :: BC065990.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATTACC GACTCACTCG CGTTTCTTTC TTATTTTTAT ATTTATTTGC TAATGTATTT 
ACTGGGATCC AGTCGAAAGA ACATGTACTG CTTGACATGA AGGCTGCCGG GAGCGAATTA 
GGTTGGTTAA CCTGGCCAAA AGAAGAGGGG TGGGAGGTTG TGCAGACGGT GGTGAACGGC 
TCTCTTCTTT ACACCTACAG CGTGTGCAAT GTTGCCAGTG ACACTGAACA AGACAACTGG 
CTTCGTACAA CCTTCATACA GCGTAGACCC GACGTCTCCC GCATGTCTGT AGAGCTGAGC 
TTCATCGTCC GCGACTGCAG CACGTTTGGA GGCTCGTCAC CCTCCTGTCG CGAGACTTTT 
GGCCTCTTCC TGGGAGAAAC CGACTCAGAT GTTGGCACAA ATTTCCGCAA GAGCCAGTTT 
CGAAAGGTAG CCACCGTAGC ACCAGACGAA GTGACAGCTG ATCGTGGAAG AGGCCGAGCT 
GATCTGCGGG TGAATGTGGA AAAGCGGAGC ATCGGGACGC TCTCCAGAAG GGGCTTCTAC 
CTTGCGTTCC AGGATTTAGG AGGCTGTGTG GCTCTCCTGG GGCTGAGGGT GTTCTACACC 
ACCTGTCCGG CCACCATGAG GAGCCTGGCC GCCTTCCCTG AGCAGGTGGC ATTGGGAGCG 
GAGGTGGAGG GCACCTGTGT GAAGGACGCA GTGGTGCTTG GAGAAGCAGC TCCTCGGATG 
TACTGCACCG CTGAGGGAGA CTGGGTCGTT CCTGTGGGCC AGTGCGTTTG CCGAGCGGGA 
TACCAGGCTG TTGGAGATTC TTGCAAAGCT TGTGAACCGG GTTTCTACAA GAGCACTGAT 
TCCAGCCAGC CTTGTGAAGT GTGTCCAGAA AACACCCAAC GTTCGGGACG GGGAGCACTG 
TTGTGCCCCT GCATGGAGGG TTTCTACCGG GCTCCCACAG ACCCAATCTC CGCTCCTTGC 
TCTTCTCTTC CTAGCCCACC CCGAGACCTG GTTTACACTC CTCTCCTGAC AGCAGGAAGT 
TTGCAGCTGC TTTGGCGCCC ACCGGCAGAC ACTGGTGGTC GGAGTGACGT CACATACAGT 
GTGGCATGCG AGCGCTGTGA GGGTGGTCTG TGTACGCTGT GTGGCGGAAG GGTGCGATTC 
GAGCCTGCGC AGACAGCCTT GAGAGCCCCC GAGGTCGTGG TTAGCGAGCT AGAGCCTCAT 
GTAAACTACA CTTTTACAGT CGAGGCCCAA AATGGCGTCT CCCAGTTCAG TCGAAAGAGA 
GCCAAGGCTA GCATCACAAC AGTGCTTCAC TTCACAGATG GCCCGAGGGT GCTGTACTTG 
AGAGTTGAAG ATCGGACCAC CAGCAGTCTG ACTTTGTCCT GGGCTGTGGA CCATCATGTG 
CAGAATCAAC CCTCCCCTCG CTACGAGCTC ATGTACCGCA AGAAGGAGGA TCCTGGAGAG 
CTGGATGTCA CCACCTACAC CGTCCTGGTT CTGGAGAAGA ACTCGGTGCC GATTAATGAC 
CTCTTACCAG GAACAAAGTA CGTGTTTCGT GTTCACACCC TGACAGCAGA AGGCCATCCC 
AGCAGCCACA GCGCGGAGCT TGAATTTGAG ACGCTGCCTC TCGCGGAATC TCGGACACAG 
AACAGTTCCA TGGTTGTCAT GGGAGCCATC GCCGGGGGCG GGGTCATGTT GCTGATTGTG 
GTGGTTATTC TGCTTCTGCA CAAAAGGAGG CTGAATTCCC ACGTCAGACG GAGGGTTGAT 
GGAGATTACT TTTCATGTCC AGAAAAACTC CTTCCGTTGA AGACCTACAT CGATCCCCAT 
ACATATGAAG ACCCCTGTGC GGCCATCCTC AAGTTTGCCA GTGAGATTCA TCCTGGTCAC 
ATCACCAAGC AGAAAGTCAT TGGTGCAGGC GAGTTTGGGG AGGTGTTTCG TGGCTCTCTG 
AAGATGCCGG GCCGCAGTGA GGTCGCAGTA GCCATAAAGA CACTGAAGCC AGGCTATACG 
GAAAAGCAGA GGCAAGACTT CCTCAGCGAG GCCAGCATCA TGGGCCAGTT TTCCCACAAG 
AACATTATCC GTCTGGAAGG TGTCGTCACC AAATTCAAAG ATGCCATGAT TATCACCGAG 
TACATGGAGA ATGGAGCTCT GGACCAGTAT TTAAGGGATC ACGATGGGGA TTTTTCCTCA 
TACCAGCTTG TGGGGATGTT GAACGGCATT GCAGCAGGAA TGAAGTATCT CTCAGACATG 
AACTACGTCC ATCGGGACCT CGCAGCTCGC AATGTGCTGG TCAACAGCAA CTTGGAGTGT 
AAAGTGTCCG ATTTCGGCTT GTCTCGAGTG CTGGAGGACT TTCCTGAGGG AACATACACC 
ACTACTGGCG GAAAGATTCC TATTCGCTGG ACTGCACCCG AGGCAATAGC TTACAGGAAG 
TTCACTTCTG CTAGTGATGT GTGGAGCTTC GGCATAGTCA TGTGGGAAGT GATGTCATTT 
GGAGAAAGAC CCTACTGGGA CATGAGCAAC CAAGAGGTAA TGAAATCCAT AAACGACGGC 
TACAGGTTGC CAGCACCGAT GGGCTGCCCA TCAGCAGTTA ATCAGCTGAT GCTGCAGTGT 
TGGATGCAGG ACCGCTCCAC TCGACCCCGC TTTGTTGATA TCGTCAACCT CCTGGAGAAG 
CTGCTGCGCA ACCCAGAGTC ACTTACATCC ATCGCCAGCA TCGACCCACG TGTCTCCATT 
CGTCTACCCA GCACTAGTGG TTGTGATGGC GCCCCCTTCA GGTCAGTGGA CGAATGGCTG 
GAATCCATCA AAATGGCCCA GTACAGAGAA ACCTTCGCCC TCGCTGGCAT TAAATCTATC 
GACCAGGTGC TGCGCTTGAA AATTGAGGAC ATCGGGAATA TTGGAGTTCG GCTGGCAGGT 
CATCAGAAGA GAATAGCATA CAGCATTTTG GGCCTGCAAG ACCCAGTGGG GGCCTTGGAT 
TTGTTTGCAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571490.1
CDS	33..2987
Translation	MDYRLTRVSFLFLYLFANVFTGIQSKEHVLLDMKAAGSELGWLTWPKEEGWEVVQTVVNGSLLYTYSVCNVASDTEQDNWLRTTFIQRRPDVSRMSVELSFIVRDCSTFGGSSPSCRETFGLFLGETDSDVGTNFRKSQFRKVATVAPDEVTADRGRGRADLRVNVEKRSIGTLSRRGFYLAFQDLGGCVALLGLRVFYTTCPATMRSLAAFPEQVALGAEVEGTCVKDAVVLGEAAPRMYCTAEGDWVVPVGQCVCRAGYQAVGDSCKACEPGFYKSTDSSQPCEVCPENTQRSGRGALLCPCMEGFYRAPTDPISAPCSSLPSPPRDLVYTPLLTAGSLQLLWRPPADTGGRSDVTYSVACERCEGGLCTLCGGRVRFEPAQTALRAPEVVVSELEPHVNYTFTVEAQNGVSQFSRKRAKASITTVLHFTDGPRVLYLRVEDRTTSSLTLSWAVDHHVQNQPSPRYELMYRKKEDPGELDVTTYTVLVLEKNSVPINDLLPGTKYVFRVHTLTAEGHPSSHSAELEFETLPLAESRTQNSSMVVMGAIAGGGVMLLIVVVILLLHKRRLNSHVRRRVDGDYFSCPEKLLPLKTYIDPHTYEDPCAAILKFASEIHPGHITKQKVIGAGEFGEVFRGSLKMPGRSEVAVAIKTLKPGYTEKQRQDFLSEASIMGQFSHKNIIRLEGVVTKFKDAMIITEYMENGALDQYLRDHDGDFSSYQLVGMLNGIAAGMKYLSDMNYVHRDLAARNVLVNSNLECKVSDFGLSRVLEDFPEGTYTTTGGKIPIRWTAPEAIAYRKFTSASDVWSFGIVMWEVMSFGERPYWDMSNQEVMKSINDGYRLPAPMGCPSAVNQLMLQCWMQDRSTRPRFVDIVNLLEKLLRNPESLTSIASIDPRVSIRLPSTSGCDGAPFRSVDEWLESIKMAQYRETFALAGIKSIDQVLRLKIEDIGNIGVRLAGHQKRIAYSILGLQDPVGALDLFAM

Target ORF information:

RefSeq Version	NM_131415.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eph receptor A2 a (epha2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131415.1

ORF Insert Sequence:

ATGGATTACC GACTCACTCG CGTTTCTTTC TTATTTTTAT ATTTATTTGC TAATGTATTT 
ACTGGGATCC AGTCGAAAGA ACATGTACTG CTTGACATGA AGGCTGCCGG GAGCGAATTA 
GGTTGGTTAA CCTGGCCAAA AGAAGAGGGG TGGGAGGTTG TGCAGACGGT GGTGAACGGC 
TCTCTTCTTT ACACCTACAG CGTGTGCAAT GTTGCCAGTG ACACTGAACA AGACAACTGG 
CTTCGTACAA CCTTCATACA GCGTAGACCC GACGTCTCCC GCATGTCTGT AGAGCTGAGC 
TTCATCGTCC GCGACTGCAG CACGTTTGGA GGCTCGTCAC CCTCCTGTCG CGAGACTTTT 
GGCCTCTTCC TGGGAGAAAC CGACTCAGAT GTTGGCACAA ATTTCCGCAA GAGCCAGTTT 
CGAAAGGTAG CCACCGTAGC ACCAGACGAA GTGACAGCTG ATCGTGGAAG AGGCCGAGCT 
GATCTGCGGG TGAATGTGGA AAAGCGGAGC ATCGGGACGC TCTCCAGAAG GGGCTTCTAC 
CTTGCGTTCC AGGATTTAGG AGGCTGTGTG GCTCTCCTGG GGCTGAGGGT GTTCTACACC 
ACCTGTCCGG CCACCATGAG GAGCCTGGCC GCCTTCCCTG AGCAGGTGGC ATTGGGAGCG 
GAGGTGGAGG GCACCTGTGT GAAGGACGCA GTGGTGCTTG GAGAAGCAGC TCCTCGGATG 
TACTGCACCG CTGAGGGAGA CTGGGTCGTT CCTGTGGGCC AGTGCGTTTG CCGAGCGGGA 
TACCAGGCTG TTGGAGATTC TTGCAAAGCT TGTGAACCGG GTTTCTACAA GAGCACTGAT 
TCCAGCCAGC CTTGTGAAGT GTGTCCAGAA AACACCCAAC GTTCGGGACG GGGAGCACTG 
TTGTGCCCCT GCATGGAGGG TTTCTACCGG GCTCCCACAG ACCCAATCTC CGCTCCTTGC 
TCTTCTCTTC CTAGCCCACC CCGAGACCTG GTTTACACTC CTCTCCTGAC AGCAGGAAGT 
TTGCAGCTGC TTTGGCGCCC ACCGGCAGAC ACTGGTGGTC GGAGTGACGT CACATACAGT 
GTGGCATGCG AGCGCTGTGA GGGTGGTCTG TGTACGCTGT GTGGCGGAAG GGTGCGATTC 
GAGCCTGCGC AGACAGCCTT GAGAGCCCCC GAGGTCGTGG TTAGCGAGCT AGAGCCTCAT 
GTAAACTACA CTTTTACAGT CGAGGCCCAA AATGGCGTCT CCCAGTTCAG TCGAAAGAGA 
GCCAAGGCTA GCATCACAAC AGTGCTTCAC TTCACAGATG GCCCGAGGGT GCTGTACTTG 
AGAGTTGAAG ATCGGACCAC CAGCAGTCTG ACTTTGTCCT GGGCTGTGGA CCATCATGTG 
CAGAATCAAC CCTCCCCTCG CTACGAGCTC ATGTACCGCA AGAAGGAGGA TCCTGGAGAG 
CTGGATGTCA CCACCTACAC CGTCCTGGTT CTGGAGAAGA ACTCGGTGCC GATTAATGAC 
CTCTTACCAG GAACAAAGTA CGTGTTTCGT GTTCACACCC TGACAGCAGA AGGCCATCCC 
AGCAGCCACA GCGCGGAGCT TGAATTTGAG ACGCTGCCTC TCGCGGAATC TCGGACACAG 
AACAGTTCCA TGGTTGTCAT GGGAGCCATC GCCGGGGGCG GGGTCATGTT GCTGATTGTG 
GTGGTTATTC TGCTTCTGCA CAAAAGGAGG CTGAATTCCC ACGTCAGACG GAGGGTTGAT 
GGAGATTACT TTTCATGTCC AGAAAAACTC CTTCCGTTGA AGACCTACAT CGATCCCCAT 
ACATATGAAG ACCCCTGTGC GGCCATCCTC AAGTTTGCCA GTGAGATTCA TCCTGGTCAC 
ATCACCAAGC AGAAAGTCAT TGGTGCAGGC GAGTTTGGGG AGGTGTTTCG TGGCTCTCTG 
AAGATGCCGG GCCGCAGTGA GGTCGCAGTA GCCATAAAGA CACTGAAGCC AGGCTATACG 
GAAAAGCAGA GGCAAGACTT CCTCAGCGAG GCCAGCATCA TGGGCCAGTT TTCCCACAAG 
AACATTATCC GTCTGGAAGG TGTCGTCACC AAATTCAAAG ATGCCATGAT TATCACCGAG 
TACATGGAGA ATGGAGCTCT GGACCAGTAT TTAAGGGATC ACGATGGGGA TTTTTCCTCA 
TACCAGCTTG TGGGGATGTT GAACGGCATT GCAGCAGGAA TGAAGTATCT CTCAGACATG 
AACTACGTCC ATCGGGACCT CGCAGCTCGC AATGTGCTGG TCAACAGCAA CTTGGAGTGT 
AAAGTGTCCG ATTTCGGCTT GTCTCGAGTG CTGGAGGACT TTCCTGAGGG AACATACACC 
ACTACTGGCG GAAAGATTCC TATTCGCTGG ACTGCACCCG AGGCAATAGC TTACAGGAAG 
TTCACTTCTG CTAGTGATGT GTGGAGCTTC GGCATAGTCA TGTGGGAAGT GATGTCATTT 
GGAGAAAGAC CCTACTGGGA CATGAGCAAC CAAGAGGTAA TGAAATCCAT AAACGACGGC 
TACAGGTTGC CAGCACCGAT GGGCTGCCCA TCAGCAGTTA ATCAGCTGAT GCTGCAGTGT 
TGGATGCAGG ACCGCTCCAC TCGACCCCGC TTTGTTGATA TCGTCAACCT CCTGGAGAAG 
CTGCTGCGCA ACCCAGAGTC ACTTACATCC ATCGCCAGCA TCGACCCACG TGTCTCCATT 
CGTCTACCCA GCACTAGTGG TTGTGATGGC GCCCCCTTCA GGTCAGTGGA CGAATGGCTG 
GAATCCATCA AAATGGCCCA GTACAGAGAA ACCTTCGCCC TCGCTGGCAT TAAATCTATC 
GACCAGGTGC TGCGCTTGAA AATTGAGGAC ATCGGGAATA TTGGAGTTCG GCTGGCAGGT 
CATCAGAAGA GAATAGCATA CAGCATTTTG GGCCTGCAAG ACCCAGTGGG GGCCTTGGAT 
TTGTTTGCAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Comparative transcriptomic profiling of hydrogen peroxide signaling networks in zebrafish and human keratinocytes: Implications toward conservation, migration and wound healing.
Scientific reports620328(2016 Feb)
Lisse TS,King BL,Rieger S

Involvement of EphA2-mediated tyrosine phosphorylation of Shp2 in Shp2-regulated activation of extracellular signal-regulated kinase.
Oncogene32(45)5292-301(2013 Nov)
Miura K,Wakayama Y,Tanino M,Orba Y,Sawa H,Hatakeyama M,Tanaka S,Sabe H,Mochizuki N

Tardbpl splicing rescues motor neuron and axonal development in a mutant tardbp zebrafish.
Human molecular genetics22(12)2376-86(2013 Jun)
Hewamadduma CA,Grierson AJ,Ma TP,Pan L,Moens CB,Ingham PW,Ramesh T,Shaw PJ

Characterisation of five novel zebrafish Eph-related receptor tyrosine kinases suggests roles in patterning the neural plate.
Development genes and evolution206(8)515-531(1997 May)
Cooke JE,Xu Q,Wilson SW,Holder N