gsk3ba cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gsk3ba
Entrez Gene ID	30654
Full Name	glycogen synthase kinase 3 beta
Synonyms	GSK-3[b],GSK3,fk80d11,wu:fb68h05,wu:fk80d11
General protein information	Preferred Names glycogen synthase kinase 3 beta Names glycogen synthase kinase-3 beta fb68h05
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009304494.2	XP_009302769.1	glycogen synthase kinase-3 beta isoform X1
XM_009304494.3	XP_009302769.1	glycogen synthase kinase-3 beta isoform X1
NM_131381.1	NP_571456.1	glycogen synthase kinase-3 beta

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GSK3B	NP_001094780.1
Mus musculus (house mouse)	Gsk3b	NP_062801.1
Rattus norvegicus (Norway rat)	Gsk3b	NP_114469.1
Caenorhabditis elegans (roundworm)	gsk-3	NP_493243.1
Xenopus tropicalis (tropical clawed frog)	gsk3b	XP_002941025.2
Canis lupus familiaris (dog)	GSK3B	XP_535751.2
Danio rerio (zebrafish)	gsk3b	NP_571456.1
Pan troglodytes (chimpanzee)	GSK3B	XP_003339094.1
Homo sapiens (human)	GSK3B	NP_002084.2
Arabidopsis thaliana (thale cress)	SK13	NP_196968.2
Oryza sativa (rice)	Os05g0134000	NP_001054564.1
Gallus gallus (chicken)	GSK3B	XP_416557.4

Gene Ontology
Bibliography

Related articles in PubMed

Met-enkephalin inhibits ROS production through Wnt/β-catenin signaling in the ZF4 cells of zebrafish.
Liu Z, Liu Y, Gu Y, Gao L, Li A, Liu D, Kang C, Pang Q, Wang X, Han Q, Yu H
Fish & shellfish immunology88432-440(2019 May)

The Glycogen Synthase Kinase-3β Inhibitor LSN 2105786 Promotes Zebrafish Fin Regeneration.
Sarmah S, Curtis C, Mahin J, Farrell M, Engler TA, Sanchez-Felix MV, Sato M, Ma YL, Chu S, Marrs JA
Biomedicines7(2)(2019 Apr)

GSK-3β at the Crossroads in Regulating Protein Synthesis and Lipid Deposition in Zebrafish.
Gu Y, Gao L, Han Q, Li A, Yu H, Liu D, Pang Q
Cells8(3)(2019 02)

Transgenic Expression of A Venous Malformation Related Mutation,
Du Z, Ma HL, Zhang ZY, Zheng JW, Wang YA
International journal of medical sciences15(4)385-394(2018)

Investigation of the Lipid-Lowering Effect of Vitamin C Through GSK-3β/β-Catenin Signaling in Zebrafish.
Liu D, Yu H, Pang Q, Zhang X
Frontiers in physiology91023(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gsk3ba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gsk3ba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37197	NM_131381.1 Latest version!	Danio rerio glycogen synthase kinase 3 beta, genome duplicate a (gsk3ba), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa39292	XM_009304494.3 Latest version!	Danio rerio glycogen synthase kinase 3 beta (gsk3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
ODa39292	XM_009304494.2	Danio rerio glycogen synthase kinase 3 beta (gsk3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gsk3ba ( NM_131381.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571456.1 Danio rerio glycogen synthase kinase 3 beta, genome duplicate a (gsk3ba), mRNA.

Price & Availability↑

CloneID	ODa37197
Clone ID Related Accession (Same CDS sequence)	NM_131381.1
Accession Version	NM_131381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Danio rerio(zebrafish)
Product	glycogen synthase kinase-3 beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ223502.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ223502.1, AB032265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGGTC GGCCCAGAAC CACCTCCTTC GCAGAAAGCT GCAAGCCAGT GCCGCAGCCG 
TCGGCATTCG GCAGCATGAA AGTCAGCAGA GATAAAGATG GCAGTAAAGT GACCACAGTG 
GTGGCGACTC CTGGACAGGG ACCTGACCGG CCGCAGGAGG TCAGCTACAC TGACACCAAG 
GTCATCGGTA ACGGCTCTTT TGGCGTGGTC TATCAGGCCA AACTCTGCGA CTCAGGAGAA 
CTGGTGGCCA TTAAGAAGGT CCTTCAGGAC AAGCGATTTA AGAACCGTGA GCTTCAGATC 
ATGAGGAAAT TAGACCACTG CAACATTGTT CGTCTGCGTT ACTTCTTCTA CTCCAGTGGA 
GATAAGAAAG ATGAGGTGTA TCTGAATTTG GTTCTGGATT ATGTTCCTGA AACCGTGTAC 
AGAGTGGCAA GACACTACAG CCGCGCTAAA CAGACTCTTC CCATGGTTTA TGTGAAGTTA 
TACATGTACC AGCTCTTCAG GAGTCTGGCC TACATCCATT CCTTTGGGAT CTGCCATCGG 
GACATAAAAC CGCAAAATCT CTTGCTGGAC CCAGACACAG CCGTGCTCAA ACTCTGCGAC 
TTTGGCAGTG CAAAGCAGTT AGTTCGTGGA GAGCCCAATG TGTCCTACAT CTGCTCACGG 
TACTACAGAG CTCCCGAACT CATCTTTGGA GCCACCGACT ACACCTCCAG CATAGATGTG 
TGGTCTGCTG GCTGTGTGCT GGCTGAACTG CTGCTCGGAC AGCCCATTTT CCCAGGTGAC 
AGCGGCGTGG ATCAGCTGGT GGAGATCATC AAGGTGTTGG GAACTCCCAC ACGAGAGCAG 
ATTCGGGAAA TGAACCCAAA CTACACCGAG TTCAAATTCC CGCAGATTAA GGCCCATCCA 
TGGACTAAGG TGTTTCGGCC ACGCACTCCC CCTGAAGCCA TTGCCTTGTG CTCCCGGCTG 
CTGGAATACA CACCGACCGC TCGACTGACT CCTCTCGAGG CCTGCGCTCA TTCCTTCTTC 
GATGAACTGC GGGAACCAAA TGTCAAACTT CCAAATGGAC GCGAGAAGCC TTCCTTATTC 
AACTTCACAA CTCAAGAGCT GTCCAGTAAC CCTACCCTGG CCTCCATCTT AATCCCCGCT 
CATGCACGTA ACCAGGCAGG AGCTTCAACT CCCACCAACC CCTCTGCCAC TTCAGATGCC 
AACAGTGGAG ATCGCAGCCA GACGACAACT GCAGCCTCCG CCTCCGCCTC TAACACCTCA 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571456.1
CDS	702..1967
Translation	MSGRPRTTSFAESCKPVPQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGDKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPMVYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELREPNVKLPNGREKPSLFNFTTQELSSNPTLASILIPAHARNQAGASTPTNPSATSDANSGDRSQTTTAASASASNTST

Target ORF information:

RefSeq Version	NM_131381.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycogen synthase kinase 3 beta, genome duplicate a (gsk3ba), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131381.1

ORF Insert Sequence:

ATGTCCGGTC GGCCCAGAAC CACCTCCTTC GCAGAAAGCT GCAAGCCAGT GCCGCAGCCG 
TCGGCATTCG GCAGCATGAA AGTCAGCAGA GATAAAGATG GCAGTAAAGT GACCACAGTG 
GTGGCGACTC CTGGACAGGG ACCTGACCGG CCGCAGGAGG TCAGCTACAC TGACACCAAG 
GTCATCGGTA ACGGCTCTTT TGGCGTGGTC TATCAGGCCA AACTCTGCGA CTCAGGAGAA 
CTGGTGGCCA TTAAGAAGGT CCTTCAGGAC AAGCGATTTA AGAACCGTGA GCTTCAGATC 
ATGAGGAAAT TAGACCACTG CAACATTGTT CGTCTGCGTT ACTTCTTCTA CTCCAGTGGA 
GATAAGAAAG ATGAGGTGTA TCTGAATTTG GTTCTGGATT ATGTTCCTGA AACCGTGTAC 
AGAGTGGCAA GACACTACAG CCGCGCTAAA CAGACTCTTC CCATGGTTTA TGTGAAGTTA 
TACATGTACC AGCTCTTCAG GAGTCTGGCC TACATCCATT CCTTTGGGAT CTGCCATCGG 
GACATAAAAC CGCAAAATCT CTTGCTGGAC CCAGACACAG CCGTGCTCAA ACTCTGCGAC 
TTTGGCAGTG CAAAGCAGTT AGTTCGTGGA GAGCCCAATG TGTCCTACAT CTGCTCACGG 
TACTACAGAG CTCCCGAACT CATCTTTGGA GCCACCGACT ACACCTCCAG CATAGATGTG 
TGGTCTGCTG GCTGTGTGCT GGCTGAACTG CTGCTCGGAC AGCCCATTTT CCCAGGTGAC 
AGCGGCGTGG ATCAGCTGGT GGAGATCATC AAGGTGTTGG GAACTCCCAC ACGAGAGCAG 
ATTCGGGAAA TGAACCCAAA CTACACCGAG TTCAAATTCC CGCAGATTAA GGCCCATCCA 
TGGACTAAGG TGTTTCGGCC ACGCACTCCC CCTGAAGCCA TTGCCTTGTG CTCCCGGCTG 
CTGGAATACA CACCGACCGC TCGACTGACT CCTCTCGAGG CCTGCGCTCA TTCCTTCTTC 
GATGAACTGC GGGAACCAAA TGTCAAACTT CCAAATGGAC GCGAGAAGCC TTCCTTATTC 
AACTTCACAA CTCAAGAGCT GTCCAGTAAC CCTACCCTGG CCTCCATCTT AATCCCCGCT 
CATGCACGTA ACCAGGCAGG AGCTTCAACT CCCACCAACC CCTCTGCCAC TTCAGATGCC 
AACAGTGGAG ATCGCAGCCA GACGACAACT GCAGCCTCCG CCTCCGCCTC TAACACCTCA 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsk3ba ( XM_009304494.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302769.1 Danio rerio glycogen synthase kinase 3 beta (gsk3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39292
Clone ID Related Accession (Same CDS sequence)	XM_009304494.2 , XM_009304494.3
Accession Version	XM_009304494.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	glycogen synthase kinase-3 beta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGTC GGCCCAGAAC CACCTCCTTC GCAGAAAGCT GCAAGCCAGT GCCGCAGCCG 
TCGGCATTCG GCAGCATGAA AGTCAGCAGA GATAAAGATG GCAGTAAAGT GACCACAGTG 
GTGGCGACTC CTGGACAGGG ACCTGACCGG CCGCAGGAGG TCAGCTACAC TGACACCAAG 
GTCATCGGTA ACGGCTCTTT TGGCGTGGTC TATCAGGCCA AACTCTGTGA CTCAGGAGAA 
CTGGTGGCCA TTAAGAAGGT TCTTCAGGAC AAGCGATTTA AGAACCGTGA GCTTCAGATC 
ATGAGGAAAT TAGACCACTG CAACATTGTT CGTCTGCGTT ACTTCTTCTA CTCCAGTGGA 
GATAAGAAAG ATGAGGTGTA TCTGAATTTG GTTCTGGATT ATGTTCCTGA AACCGTGTAC 
AGAGTGGCAA GACACTACAG CCGCGCTAAA CAGACTCTTC CCATGGTTTA TGTGAAGTTG 
TACATGTACC AGCTCTTCAG GAGTCTGGCC TACATCCATT CCTTTGGGAT CTGCCATCGG 
GACATAAAAC CGCAAAATCT CTTGCTGGAC CCAGACACAG CCGTGCTCAA ACTCTGTGAC 
TTTGGCAGCG CTAAGCAGTT AGTACGTGGA GAGCCCAATG TGTCCTACAT CTGCTCACGG 
TACTACAGAG CTCCCGAACT CATCTTTGGA GCCACCGACT ACACCTCCAG CATAGATGTG 
TGGTCTGCTG GCTGTGTGCT GGCTGAACTG CTGCTCGGAC AGCCCATTTT CCCAGGTGAC 
AGCGGCGTGG ATCAGCTGGT GGAGATCATC AAGGTGTTGG GAACTCCCAC ACGAGAGCAG 
ATTCGGGAAA TGAACCCAAA CTACACCGAG TTCAAATTCC CGCAGATTAA GGCCCATCCA 
TGGACTAAGG TGAGTAAACA GGTGTTTCGG CCACGCACTC CCCCTGAAGC CATTGCCTTG 
TGCTCCCGGC TGCTGGAATA CACACCGACC GCTCGACTGA CTCCTCTCGA GGCCTGCGCT 
CATTCCTTCT TCGATGAACT GCGGGAACCA AATGTCAAAC TTCCAAATGG ACGCGAGAAG 
CCTTCCTTAT TCAACTTCAC AACTCAAGAG CTGTCCAGTA ACCCTACCCT GGCCTCCATC 
TTAATCCCCG CTCATGCACG TAACCAGGCA GGAGCTTCAA CTCCCACCAA CCCCTCTGCC 
ACTTCAGATG CCAACAGTGG AGATCGCAGC CAGACGACAA CTGCAGCCTC CGCCTCCGCC 
TCTAACACCT CAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302769.1
CDS	852..2129
Translation	MSGRPRTTSFAESCKPVPQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGDKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPMVYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVSKQVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELREPNVKLPNGREKPSLFNFTTQELSSNPTLASILIPAHARNQAGASTPTNPSATSDANSGDRSQTTTAASASASNTST

Target ORF information:

RefSeq Version	XM_009304494.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glycogen synthase kinase 3 beta (gsk3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304494.2

ORF Insert Sequence:

ATGTCCGGTC GGCCCAGAAC CACCTCCTTC GCAGAAAGCT GCAAGCCAGT GCCGCAGCCG 
TCGGCATTCG GCAGCATGAA AGTCAGCAGA GATAAAGATG GCAGTAAAGT GACCACAGTG 
GTGGCGACTC CTGGACAGGG ACCTGACCGG CCGCAGGAGG TCAGCTACAC TGACACCAAG 
GTCATCGGTA ACGGCTCTTT TGGCGTGGTC TATCAGGCCA AACTCTGTGA CTCAGGAGAA 
CTGGTGGCCA TTAAGAAGGT TCTTCAGGAC AAGCGATTTA AGAACCGTGA GCTTCAGATC 
ATGAGGAAAT TAGACCACTG CAACATTGTT CGTCTGCGTT ACTTCTTCTA CTCCAGTGGA 
GATAAGAAAG ATGAGGTGTA TCTGAATTTG GTTCTGGATT ATGTTCCTGA AACCGTGTAC 
AGAGTGGCAA GACACTACAG CCGCGCTAAA CAGACTCTTC CCATGGTTTA TGTGAAGTTG 
TACATGTACC AGCTCTTCAG GAGTCTGGCC TACATCCATT CCTTTGGGAT CTGCCATCGG 
GACATAAAAC CGCAAAATCT CTTGCTGGAC CCAGACACAG CCGTGCTCAA ACTCTGTGAC 
TTTGGCAGCG CTAAGCAGTT AGTACGTGGA GAGCCCAATG TGTCCTACAT CTGCTCACGG 
TACTACAGAG CTCCCGAACT CATCTTTGGA GCCACCGACT ACACCTCCAG CATAGATGTG 
TGGTCTGCTG GCTGTGTGCT GGCTGAACTG CTGCTCGGAC AGCCCATTTT CCCAGGTGAC 
AGCGGCGTGG ATCAGCTGGT GGAGATCATC AAGGTGTTGG GAACTCCCAC ACGAGAGCAG 
ATTCGGGAAA TGAACCCAAA CTACACCGAG TTCAAATTCC CGCAGATTAA GGCCCATCCA 
TGGACTAAGG TGAGTAAACA GGTGTTTCGG CCACGCACTC CCCCTGAAGC CATTGCCTTG 
TGCTCCCGGC TGCTGGAATA CACACCGACC GCTCGACTGA CTCCTCTCGA GGCCTGCGCT 
CATTCCTTCT TCGATGAACT GCGGGAACCA AATGTCAAAC TTCCAAATGG ACGCGAGAAG 
CCTTCCTTAT TCAACTTCAC AACTCAAGAG CTGTCCAGTA ACCCTACCCT GGCCTCCATC 
TTAATCCCCG CTCATGCACG TAACCAGGCA GGAGCTTCAA CTCCCACCAA CCCCTCTGCC 
ACTTCAGATG CCAACAGTGG AGATCGCAGC CAGACGACAA CTGCAGCCTC CGCCTCCGCC 
TCTAACACCT CAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsk3ba ( XM_009304494.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302769.1 Danio rerio glycogen synthase kinase 3 beta (gsk3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39292
Clone ID Related Accession (Same CDS sequence)	XM_009304494.2 , XM_009304494.3
Accession Version	XM_009304494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	glycogen synthase kinase-3 beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304494.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGTC GGCCCAGAAC CACCTCCTTC GCAGAAAGCT GCAAGCCAGT GCCGCAGCCG 
TCGGCATTCG GCAGCATGAA AGTCAGCAGA GATAAAGATG GCAGTAAAGT GACCACAGTG 
GTGGCGACTC CTGGACAGGG ACCTGACCGG CCGCAGGAGG TCAGCTACAC TGACACCAAG 
GTCATCGGTA ACGGCTCTTT TGGCGTGGTC TATCAGGCCA AACTCTGTGA CTCAGGAGAA 
CTGGTGGCCA TTAAGAAGGT TCTTCAGGAC AAGCGATTTA AGAACCGTGA GCTTCAGATC 
ATGAGGAAAT TAGACCACTG CAACATTGTT CGTCTGCGTT ACTTCTTCTA CTCCAGTGGA 
GATAAGAAAG ATGAGGTGTA TCTGAATTTG GTTCTGGATT ATGTTCCTGA AACCGTGTAC 
AGAGTGGCAA GACACTACAG CCGCGCTAAA CAGACTCTTC CCATGGTTTA TGTGAAGTTG 
TACATGTACC AGCTCTTCAG GAGTCTGGCC TACATCCATT CCTTTGGGAT CTGCCATCGG 
GACATAAAAC CGCAAAATCT CTTGCTGGAC CCAGACACAG CCGTGCTCAA ACTCTGTGAC 
TTTGGCAGCG CTAAGCAGTT AGTACGTGGA GAGCCCAATG TGTCCTACAT CTGCTCACGG 
TACTACAGAG CTCCCGAACT CATCTTTGGA GCCACCGACT ACACCTCCAG CATAGATGTG 
TGGTCTGCTG GCTGTGTGCT GGCTGAACTG CTGCTCGGAC AGCCCATTTT CCCAGGTGAC 
AGCGGCGTGG ATCAGCTGGT GGAGATCATC AAGGTGTTGG GAACTCCCAC ACGAGAGCAG 
ATTCGGGAAA TGAACCCAAA CTACACCGAG TTCAAATTCC CGCAGATTAA GGCCCATCCA 
TGGACTAAGG TGAGTAAACA GGTGTTTCGG CCACGCACTC CCCCTGAAGC CATTGCCTTG 
TGCTCCCGGC TGCTGGAATA CACACCGACC GCTCGACTGA CTCCTCTCGA GGCCTGCGCT 
CATTCCTTCT TCGATGAACT GCGGGAACCA AATGTCAAAC TTCCAAATGG ACGCGAGAAG 
CCTTCCTTAT TCAACTTCAC AACTCAAGAG CTGTCCAGTA ACCCTACCCT GGCCTCCATC 
TTAATCCCCG CTCATGCACG TAACCAGGCA GGAGCTTCAA CTCCCACCAA CCCCTCTGCC 
ACTTCAGATG CCAACAGTGG AGATCGCAGC CAGACGACAA CTGCAGCCTC CGCCTCCGCC 
TCTAACACCT CAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302769.1
CDS	782..2059
Translation	MSGRPRTTSFAESCKPVPQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGDKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPMVYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVSKQVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELREPNVKLPNGREKPSLFNFTTQELSSNPTLASILIPAHARNQAGASTPTNPSATSDANSGDRSQTTTAASASASNTST

Target ORF information:

RefSeq Version	XM_009304494.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycogen synthase kinase 3 beta (gsk3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304494.3

ORF Insert Sequence:

ATGTCCGGTC GGCCCAGAAC CACCTCCTTC GCAGAAAGCT GCAAGCCAGT GCCGCAGCCG 
TCGGCATTCG GCAGCATGAA AGTCAGCAGA GATAAAGATG GCAGTAAAGT GACCACAGTG 
GTGGCGACTC CTGGACAGGG ACCTGACCGG CCGCAGGAGG TCAGCTACAC TGACACCAAG 
GTCATCGGTA ACGGCTCTTT TGGCGTGGTC TATCAGGCCA AACTCTGTGA CTCAGGAGAA 
CTGGTGGCCA TTAAGAAGGT TCTTCAGGAC AAGCGATTTA AGAACCGTGA GCTTCAGATC 
ATGAGGAAAT TAGACCACTG CAACATTGTT CGTCTGCGTT ACTTCTTCTA CTCCAGTGGA 
GATAAGAAAG ATGAGGTGTA TCTGAATTTG GTTCTGGATT ATGTTCCTGA AACCGTGTAC 
AGAGTGGCAA GACACTACAG CCGCGCTAAA CAGACTCTTC CCATGGTTTA TGTGAAGTTG 
TACATGTACC AGCTCTTCAG GAGTCTGGCC TACATCCATT CCTTTGGGAT CTGCCATCGG 
GACATAAAAC CGCAAAATCT CTTGCTGGAC CCAGACACAG CCGTGCTCAA ACTCTGTGAC 
TTTGGCAGCG CTAAGCAGTT AGTACGTGGA GAGCCCAATG TGTCCTACAT CTGCTCACGG 
TACTACAGAG CTCCCGAACT CATCTTTGGA GCCACCGACT ACACCTCCAG CATAGATGTG 
TGGTCTGCTG GCTGTGTGCT GGCTGAACTG CTGCTCGGAC AGCCCATTTT CCCAGGTGAC 
AGCGGCGTGG ATCAGCTGGT GGAGATCATC AAGGTGTTGG GAACTCCCAC ACGAGAGCAG 
ATTCGGGAAA TGAACCCAAA CTACACCGAG TTCAAATTCC CGCAGATTAA GGCCCATCCA 
TGGACTAAGG TGAGTAAACA GGTGTTTCGG CCACGCACTC CCCCTGAAGC CATTGCCTTG 
TGCTCCCGGC TGCTGGAATA CACACCGACC GCTCGACTGA CTCCTCTCGA GGCCTGCGCT 
CATTCCTTCT TCGATGAACT GCGGGAACCA AATGTCAAAC TTCCAAATGG ACGCGAGAAG 
CCTTCCTTAT TCAACTTCAC AACTCAAGAG CTGTCCAGTA ACCCTACCCT GGCCTCCATC 
TTAATCCCCG CTCATGCACG TAACCAGGCA GGAGCTTCAA CTCCCACCAA CCCCTCTGCC 
ACTTCAGATG CCAACAGTGG AGATCGCAGC CAGACGACAA CTGCAGCCTC CGCCTCCGCC 
TCTAACACCT CAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Met-enkephalin inhibits ROS production through Wnt/β-catenin signaling in the ZF4 cells of zebrafish.
Fish & shellfish immunology88432-440(2019 May)
Liu Z,Liu Y,Gu Y,Gao L,Li A,Liu D,Kang C,Pang Q,Wang X,Han Q,Yu H

The Glycogen Synthase Kinase-3β Inhibitor LSN 2105786 Promotes Zebrafish Fin Regeneration.
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