MIXL1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIXL1
Entrez Gene ID	30115
Full Name	Mix paired-like homeobox
Synonyms	bon,bon/mixer,mix,mix-like
General protein information	Preferred Names Mix paired-like homeobox Names homeobox protein MIXL1 bonnie and clyde mixer
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130940.3	NP_571015.3	homeobox protein MIXL1
XM_005170170.3	XP_005170227.1	homeobox protein MIXL1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100330956	XP_002664678.1
Danio rerio (zebrafish)	bon	NP_571015.2

Gene Ontology
Bibliography

Related articles in PubMed

CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium.
Mönnich M, Borgeskov L, Breslin L, Jakobsen L, Rogowski M, Doganli C, Schrøder JM, Mogensen JB, Blinkenkjær L, Harder LM, Lundberg E, Geimer S, Christensen ST, Andersen JS, Larsen LA, Pedersen LB
Cell reports22(10)2584-2592(2018 03)

Identification of transcripts potentially involved in neural tube closure using RNA sequencing.
Kindt LM, Coughlin AR, Perosino TR, Ersfeld HN, Hampton M, Liang JO
Genesis (New York, N.Y. : 2000)56(3)e23096(2018 03)

Maternal Nanog is required for zebrafish embryo architecture and for cell viability during gastrulation.
Veil M, Schaechtle MA, Gao M, Kirner V, Buryanova L, Grethen R, Onichtchouk D
Development (Cambridge, England)145(1)(2018 01)

Long-Range Signaling Activation and Local Inhibition Separate the Mesoderm and Endoderm Lineages.
van Boxtel AL, Economou AD, Heliot C, Hill CS
Developmental cell44(2)179-191.e5(2018 01)

Opposite effects of Activin type 2 receptor ligands on cardiomyocyte proliferation during development and repair.
Dogra D, Ahuja S, Kim HT, Rasouli SJ, Stainier DYR, Reischauer S
Nature communications8(1)1902(2017 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MIXL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIXL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa25166	NM_130940.3 Latest version!	Danio rerio Mix paired-like homeobox (mixl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa25166	XM_005170170.3 Latest version!	Danio rerio Mix paired-like homeobox (mixl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIXL1 ( NM_130940.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571015.3 Danio rerio Mix paired-like homeobox (mixl1), mRNA.

Price & Availability↑

CloneID	ODa25166
Clone ID Related Accession (Same CDS sequence)	NM_130940.3 , XM_005170170.3
Accession Version	NM_130940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1533312000000
Organism	Danio rerio(zebrafish)
Product	homeobox protein MIXL1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649436.12. On Mar 20, 2015 this sequence version replaced NM_130940.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC097091.1, AF121771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505381 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGTCG TGCACGGAAA CCTCGGGAAT GGACATTTAA AACAATTCCA GCTTTTTCCA 
AACGCGGATA TGGAGAGGAG GACGGGCATG CACACATCAG ATTATGTGCT TCAGGACAGG 
ATGAAGTTCC TGGATGGTGC CTCCAGGGCA GACAACGTCA GTTTGTTTAC ACACCGCAGA 
AAACGCACAA ACTTCACGCA GCAGCAGATC GACGTGCTGG AGAAAGTTTA CCTGGACACC 
AAATACCCTG ATATTTATTT ACGAGAGAAG CTTGAGGCAC TCACCGGCTT GCCAGAATCG 
AGAATTCAGG TCTGGTTCCA GAACCGAAGG GCGAAGTCTC GGCGCCAGGT GGGAATTTCA 
ATCCCAAACA AGACGAGCGG AAACATCCTG ACCCCCAACA ATCTCCTTAT GCACCAGTTT 
ACCACACATC AGAACCACAG CGGCCTGGAG AACTTACAAA GAACCTCAAC ATTTACAGCA 
GACAGCTTCC ATCAGCAGCT CATCAACTCT TCAGAGGAGA AAATCAGCAC CATTAAAGGT 
GACATATACG ACCCAACCAC CATCCCATGC ATGTTCAGCA GAACCCAGGA GAATCCCATC 
AAAACTGATC ACCTGAGTGT GGTGGTGCCG CGCAGTTACA CACAGCAGTA CCCAAAGGAA 
CACGAACAGT TCACACATTC AGCAAACCAC GCTAAATCAA CAATGAAGCA GTTTTTGGTG 
GAGTATGACA ACTTCCCTCC TAACAAGACC ATCGGGCCGG AGATGAAGGT GGTCATTCCT 
CCTCTGCCAT CTCAGAGTAA CTTCATGATG TCTTCATCAT CCCCTAAACA CATCGCCTGT 
TCTGTACAAA ACATGTCGGT GCAGACACAA CCAGAGCTTT TTGCTACTTT TTCTCCAATC 
AGAGCAAGCG AAGCCGTCGA GTTCTCCGAT TCTGACTCTG ATTGGGAAAG AGACGCCATG 
TCTGGTTTTA ATGGCTTCAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571015.3
CDS	37..1020
Translation	MAVVHGNLGNGHLKQFQLFPNADMERRTGMHTSDYVLQDRMKFLDGASRADNVSLFTHRRKRTNFTQQQIDVLEKVYLDTKYPDIYLREKLEALTGLPESRIQVWFQNRRAKSRRQVGISIPNKTSGNILTPNNLLMHQFTTHQNHSGLENLQRTSTFTADSFHQQLINSSEEKISTIKGDIYDPTTIPCMFSRTQENPIKTDHLSVVVPRSYTQQYPKEHEQFTHSANHAKSTMKQFLVEYDNFPPNKTIGPEMKVVIPPLPSQSNFMMSSSSPKHIACSVQNMSVQTQPELFATFSPIRASEAVEFSDSDSDWERDAMSGFNGFI

Target ORF information:

RefSeq Version	NM_130940.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Mix paired-like homeobox (mixl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130940.3

ORF Insert Sequence:

ATGGCAGTCG TGCACGGAAA CCTCGGGAAT GGACATTTAA AACAATTCCA GCTTTTTCCA 
AACGCGGATA TGGAGAGGAG GACGGGCATG CACACATCAG ATTATGTGCT TCAGGACAGG 
ATGAAGTTCC TGGATGGTGC CTCCAGGGCA GACAACGTCA GTTTGTTTAC ACACCGCAGA 
AAACGCACAA ACTTCACGCA GCAGCAGATC GACGTGCTGG AGAAAGTTTA CCTGGACACC 
AAATACCCTG ATATTTATTT ACGAGAGAAG CTTGAGGCAC TCACCGGCTT GCCAGAATCG 
AGAATTCAGG TCTGGTTCCA GAACCGAAGG GCGAAGTCTC GGCGCCAGGT GGGAATTTCA 
ATCCCAAACA AGACGAGCGG AAACATCCTG ACCCCCAACA ATCTCCTTAT GCACCAGTTT 
ACCACACATC AGAACCACAG CGGCCTGGAG AACTTACAAA GAACCTCAAC ATTTACAGCA 
GACAGCTTCC ATCAGCAGCT CATCAACTCT TCAGAGGAGA AAATCAGCAC CATTAAAGGT 
GACATATACG ACCCAACCAC CATCCCATGC ATGTTCAGCA GAACCCAGGA GAATCCCATC 
AAAACTGATC ACCTGAGTGT GGTGGTGCCG CGCAGTTACA CACAGCAGTA CCCAAAGGAA 
CACGAACAGT TCACACATTC AGCAAACCAC GCTAAATCAA CAATGAAGCA GTTTTTGGTG 
GAGTATGACA ACTTCCCTCC TAACAAGACC ATCGGGCCGG AGATGAAGGT GGTCATTCCT 
CCTCTGCCAT CTCAGAGTAA CTTCATGATG TCTTCATCAT CCCCTAAACA CATCGCCTGT 
TCTGTACAAA ACATGTCGGT GCAGACACAA CCAGAGCTTT TTGCTACTTT TTCTCCAATC 
AGAGCAAGCG AAGCCGTCGA GTTCTCCGAT TCTGACTCTG ATTGGGAAAG AGACGCCATG 
TCTGGTTTTA ATGGCTTCAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIXL1 ( XM_005170170.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005170227.1 Danio rerio Mix paired-like homeobox (mixl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25166
Clone ID Related Accession (Same CDS sequence)	NM_130940.3 , XM_005170170.3
Accession Version	XM_005170170.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	homeobox protein MIXL1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170170.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTCG TGCACGGAAA CCTCGGGAAT GGACATTTAA AACAATTCCA GCTTTTTCCA 
AACGCGGATA TGGAGAGGAG GACGGGCATG CACACATCAG ATTATGTGCT TCAGGACAGG 
ATGAAGTTCC TGGATGGTGC CTCCAGGGCA GACAACGTCA GTTTGTTTAC ACACCGCAGA 
AAACGCACAA ACTTCACGCA GCAGCAGATC GACGTGCTGG AGAAAGTTTA CCTGGACACC 
AAATACCCTG ATATTTATTT ACGAGAGAAG CTTGAGGCAC TCACCGGCTT GCCAGAATCG 
AGAATTCAGG TCTGGTTCCA GAACCGAAGG GCGAAGTCTC GGCGCCAGGT GGGAATTTCA 
ATCCCAAACA AGACGAGCGG AAACATCCTG ACCCCCAACA ATCTCCTTAT GCACCAGTTT 
ACCACACATC AGAACCACAG CGGCCTGGAG AACTTACAAA GAACCTCAAC ATTTACAGCA 
GACAGCTTCC ATCAGCAGCT CATCAACTCT TCAGAGGAGA AAATCAGCAC CATTAAAGGT 
GACATATACG ACCCAACCAC CATCCCATGC ATGTTCAGCA GAACCCAGGA GAATCCCATC 
AAAACTGATC ACCTGAGTGT GGTGGTGCCG CGCAGTTACA CACAGCAGTA CCCAAAGGAA 
CACGAACAGT TCACACATTC AGCAAACCAC GCTAAATCAA CAATGAAGCA GTTTTTGGTG 
GAGTATGACA ACTTCCCTCC TAACAAGACC ATCGGGCCGG AGATGAAGGT GGTCATTCCT 
CCTCTGCCAT CTCAGAGTAA CTTCATGATG TCTTCATCAT CCCCTAAACA CATCGCCTGT 
TCTGTACAAA ACATGTCGGT GCAGACACAA CCAGAGCTTT TTGCTACTTT TTCTCCAATC 
AGAGCAAGCG AAGCCGTCGA GTTCTCCGAT TCTGACTCTG ATTGGGAAAG AGACGCCATG 
TCTGGTTTTA ATGGCTTCAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170227.1
CDS	188..1171
Translation	MAVVHGNLGNGHLKQFQLFPNADMERRTGMHTSDYVLQDRMKFLDGASRADNVSLFTHRRKRTNFTQQQIDVLEKVYLDTKYPDIYLREKLEALTGLPESRIQVWFQNRRAKSRRQVGISIPNKTSGNILTPNNLLMHQFTTHQNHSGLENLQRTSTFTADSFHQQLINSSEEKISTIKGDIYDPTTIPCMFSRTQENPIKTDHLSVVVPRSYTQQYPKEHEQFTHSANHAKSTMKQFLVEYDNFPPNKTIGPEMKVVIPPLPSQSNFMMSSSSPKHIACSVQNMSVQTQPELFATFSPIRASEAVEFSDSDSDWERDAMSGFNGFI

Target ORF information:

RefSeq Version	XM_005170170.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Mix paired-like homeobox (mixl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170170.3

ORF Insert Sequence:

ATGGCAGTCG TGCACGGAAA CCTCGGGAAT GGACATTTAA AACAATTCCA GCTTTTTCCA 
AACGCGGATA TGGAGAGGAG GACGGGCATG CACACATCAG ATTATGTGCT TCAGGACAGG 
ATGAAGTTCC TGGATGGTGC CTCCAGGGCA GACAACGTCA GTTTGTTTAC ACACCGCAGA 
AAACGCACAA ACTTCACGCA GCAGCAGATC GACGTGCTGG AGAAAGTTTA CCTGGACACC 
AAATACCCTG ATATTTATTT ACGAGAGAAG CTTGAGGCAC TCACCGGCTT GCCAGAATCG 
AGAATTCAGG TCTGGTTCCA GAACCGAAGG GCGAAGTCTC GGCGCCAGGT GGGAATTTCA 
ATCCCAAACA AGACGAGCGG AAACATCCTG ACCCCCAACA ATCTCCTTAT GCACCAGTTT 
ACCACACATC AGAACCACAG CGGCCTGGAG AACTTACAAA GAACCTCAAC ATTTACAGCA 
GACAGCTTCC ATCAGCAGCT CATCAACTCT TCAGAGGAGA AAATCAGCAC CATTAAAGGT 
GACATATACG ACCCAACCAC CATCCCATGC ATGTTCAGCA GAACCCAGGA GAATCCCATC 
AAAACTGATC ACCTGAGTGT GGTGGTGCCG CGCAGTTACA CACAGCAGTA CCCAAAGGAA 
CACGAACAGT TCACACATTC AGCAAACCAC GCTAAATCAA CAATGAAGCA GTTTTTGGTG 
GAGTATGACA ACTTCCCTCC TAACAAGACC ATCGGGCCGG AGATGAAGGT GGTCATTCCT 
CCTCTGCCAT CTCAGAGTAA CTTCATGATG TCTTCATCAT CCCCTAAACA CATCGCCTGT 
TCTGTACAAA ACATGTCGGT GCAGACACAA CCAGAGCTTT TTGCTACTTT TTCTCCAATC 
AGAGCAAGCG AAGCCGTCGA GTTCTCCGAT TCTGACTCTG ATTGGGAAAG AGACGCCATG 
TCTGGTTTTA ATGGCTTCAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium.
Cell reports22(10)2584-2592(2018 03)
Mönnich M,Borgeskov L,Breslin L,Jakobsen L,Rogowski M,Doganli C,Schrøder JM,Mogensen JB,Blinkenkjær L,Harder LM,Lundberg E,Geimer S,Christensen ST,Andersen JS,Larsen LA,Pedersen LB

Identification of transcripts potentially involved in neural tube closure using RNA sequencing.
Genesis (New York, N.Y. : 2000)56(3)e23096(2018 03)
Kindt LM,Coughlin AR,Perosino TR,Ersfeld HN,Hampton M,Liang JO

Maternal Nanog is required for zebrafish embryo architecture and for cell viability during gastrulation.
Development (Cambridge, England)145(1)(2018 01)
Veil M,Schaechtle MA,Gao M,Kirner V,Buryanova L,Grethen R,Onichtchouk D

Long-Range Signaling Activation and Local Inhibition Separate the Mesoderm and Endoderm Lineages.
Developmental cell44(2)179-191.e5(2018 01)
van Boxtel AL,Economou AD,Heliot C,Hill CS

Opposite effects of Activin type 2 receptor ligands on cardiomyocyte proliferation during development and repair.
Nature communications8(1)1902(2017 12)
Dogra D,Ahuja S,Kim HT,Rasouli SJ,Stainier DYR,Reischauer S

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MIXL1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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